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27 results on '"Jin, Huiyi"'

15. Electrodeposition of Zn--Ni alloy in choline chloride--malonic acid ionic liquid.

Author

BU Luxia, LIU Hengyue, JIN Huiyi, and YAN Zonglan

Abstract

Zn--Ni alloy coating was electrodeposited potentiostatically in an ionic liquid mainly composed of choline chloride and malonic acid. The effect of electrodeposition potential on the porosity, corrosion resistance, adhesion strength, and surface morphology of Zn--Ni alloy coating was studied. The results showed that the Zn--Ni alloy coating electroplated at -1.2 V was uniform and compact with a low porosity and optimal corrosion resistance. [ABSTRACT FROM AUTHOR]

Published
2021
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18. Effects of a thrombomodulin-derived peptide on monocyte adhesion and intercellular adhesion molecule-1 expression in lipopolysaccharide-induced endothelial cells

Author

Xu, Yan, Xu, Xun, Jin, Huiyi, Yang, Xiaolu, Gu, Qing, and Liu, Kun

Subjects
Inflammation, Lipopolysaccharides, MAP Kinase Signaling System, Thrombomodulin, Endothelial Cells, U937 Cells, Intercellular Adhesion Molecule-1, Monocytes, Peptide Fragments, Cell Adhesion, Human Umbilical Vein Endothelial Cells, Humans, RNA, Messenger, Research Article
Abstract

Purpose It has been documented that GC31, a 31-animo acid peptide from human thrombomodulin, has potent anti-inflammatory properties in endotoxin-induced uveitis and lipopolysaccharide (LPS)-induced RAW264.7 cells, while the role of GC31 in the endothelial cells has not yet been fully understood. Therefore, the aim of this study was to explore the effect of GC31 on intercellular adhesion molecule-1 (ICAM-1) expression in LPS-activated endothelial cells. Methods Human umbilical vein endothelial cells (HUVECs) were incubated with LPS (1 μg/ml) and peptide GC31 or control peptide VP30 simultaneously. ICAM-1 messenger RNA and protein levels were evaluated with real-time PCR and western blot. The adhesion of U937 cells labeled with CM-H2DCFDA to HUVECs was examined with fluorescence microscope. Extracellular signal-regulated kinase-1/2 (ERK1/2) and p38 mitogen-activated protein kinase (MAPK) activation, inhibitor of nuclear factor kappa B alpha (IκBα) degradation, and nuclear factor kappa B (NF-κB) nuclear translocation were detected with western blot. Results Upon LPS stimulation, GC31 suppressed the mRNA and protein expression of ICAM-1 in HUVECs and remarkably reduced monocyte-endothelial cell adhesion in a dose-dependent manner. Furthermore, GC31 significantly inhibited the degradation of IκBα and nuclear translocation of NF-κB and moderately blocked the activation of p38 MAPK and ERK1/2 in activated HUVECs. Conclusions Our results suggested that GC31 suppressed LPS-mediated ICAM-1 expression by inhibiting the activation of NF-κB and partially by attenuating the activity of ERK1/2 and p38 MAPK in vascular endothelium, which may contribute to ameliorating vascular inflammatory diseases, such as uveitis.

Published
2013

20. Elevated plasma and vitreous levels of leucine‐rich‐α2‐glycoprotein are associated with diabetic retinopathy progression.

Author

Chen, Chong, Chen, Xia, Huang, Hengye, Han, Changjing, Qu, Yuan, Jin, Huiyi, Niu, Tian, Zhang, Yuan, Liu, Kun, and Xu, Xun

Subjects
DIABETIC retinopathy, TYPE 2 diabetes, RECEIVER operating characteristic curves, ENZYME-linked immunosorbent assay
Abstract

Purpose: To investigate the association of plasma and vitreous leucine‐rich‐α2‐glycoprotein (LRG1) with diabetic retinopathy (DR) progression. Methods: A total of 86 outpatients and 33 inpatients were recruited. Outpatients with type 2 diabetes mellitus (T2DM) were classified as T2DM without DR (n = 22), nonproliferative DR (NPDR) (n = 20) and proliferative DR (PDR) (n = 22) based on international clinical DR severity scales. A total of 86 plasma and 33 vitreous samples were collected and subjected to enzyme‐linked immunosorbent assay. The diagnostic value of plasma LRG1 was tested using receiver operating characteristic (ROC) curves. Results: Plasma LRG1 in PDR patients (9025 ± 1870 pg/ml) was significantly increased as compared with controls (5975 ± 2022 pg/ml), T2DM without DR (6550 ± 2359 pg/ml) and NPDR patients (6550 ± 2359 pg/ml) (p < 0.0001). Vitreous LRG1 in PDR patients was elevated by approximately 4.3‐fold than that in controls (562.1 ± 273.5 ng/ml versus 130.0 ± 102.8 ng/ml, p = 0.000). The area under the ROC curve value for plasma LRG1 was 0.786 (p < 0.0001). The maximal Youden index was 0.4372 and the optimal cut‐off value of LRG1 was 7357.043 pg/ml with 81.82% sensitivity and 61.90% specificity. Conclusion: Plasma and vitreous LRG1 levels were elevated in patients with PDR. Leucine‐rich‐α2‐glycoprotein (LRG1) might be a potential risk‐warning marker for PDR. [ABSTRACT FROM AUTHOR]

Published
2019
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