Hydrogen plays a significant role in the future energy systems because it is a clean energy career. Biomass gasification can provide a promising way to produce renewable and reliable hydrogen with large quantities. However, the low conversion to hydrogen with low energy efficiency is one of the main challenges for the development of the process. Introduction of catalyst is an effective method to achieve high hydrogen yield and energy efficiency. However, there are several challenges for catalyst development such as coke deposition and sintering for the biomass gasification. In this work, Ni-based catalyst was investigated with meso-porous and metal oxide supports, which are important catalyst supports in the heterogeneous catalysis area. Two stage fixed bed reaction system was used to evaluate the catalyst for the hydrogen production. In Chapter 3, influences of calcium addition to Ni-Ca-Mg-Al were investigated for hydrogen production from biomass gasification. In Chapter 4, effects of magnesium were studied on Ni-Ca-Mg-Al catalyst for hydrogen generation. In Chapter 5, Ni supported on different MCM-41 porous materials (H-[Al] MCM-41, Na-[Si] MCM-41, H-[Si] MCM-41) with different support acidities were studied for their influences on hydrogen generation. In addition, a Ni/SiO2 catalyst was also used to compare to the porous support. The results showed that both type of catalysts can produce hydrogen as high as 21.5 (mmol H2 g-1 sample). The increase of acidity in the Ni/MCM-41 catalyst enhanced the hydrogen production compare with the other Ni/MCM-41 catalyst. The Ni/SiO2 catalyst produced reasonable amount of hydrogen compared with Ni/MCM-41 catalysts modified by Na; however it had much higher coke deposition due to the presence of large NiO particles, which were easily deactivated. The addition of Ca into the Ni-Mg-Al catalyst generated the catalyst with different fraction of small NiO, bulk NiO and NiAl2O4 phase. The results suggested that small NiO particles favored the hydrogen production; however, the fraction of small NiO should be controlled, and it also promoted coke formation on the catalyst. NiAl2O4 spinel phases were positive for coke prohibition during the catalytic biomass gasification; however it showed less catalytic activity for hydrogen production compared with small NiO particles. The enhancement of hydrogen production by Ca addition due to in-situ CO2 adsorption was not clear; further studies are suggested.