1. Mapping of Two Major QTLs Controlling Flowering Time in Brassica napus Using a High-Density Genetic Map
- Author
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Lei Chen, Weixia Lei, Wangfei He, Yifan Wang, Jie Tian, Jihui Gong, Bing Hao, Xinxin Cheng, Yingjie Shu, and Zhixiong Fan
- Subjects
rapeseed ,flowering time ,quantitative trait locus ,mapping ,Botany ,QK1-989 - Abstract
Research on the flowering habit of rapeseed is important for the selection of varieties adapted to specific ecological environments. Here, quantitative trait loci (QTL) for the days-to-flowering trait were identified using a doubled haploid population of 178 lines derived from a cross between the winter type SGDH284 and the semi-winter type 158A. A linkage map encompassing 3268.01 cM was constructed using 2777 bin markers obtained from next-generation sequencing. The preliminary mapping results revealed 56 QTLs for the days to flowering in the six replicates in the three environments. Twelve consensus QTLs were identified by a QTL meta-analysis, two of which (cqDTF-C02 and cqDTF-C06) were designated as major QTLs. Based on the micro-collinearity of the target regions between B. napus and Arabidopsis, four genes possibly related to flowering time were identified in the cqDTF-C02 interval, and only one gene possibly related to flowering time was identified in the cqDTF-C06 interval. A tightly linked insertion–deletion marker for the cqFT-C02 locus was developed. These findings will aid the breeding of early maturing B. napus varieties.
- Published
- 2022
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