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1. Optimal cut-offs of depression screening tools during the COVID-19 pandemic: a systematic review

Author

Jieru Zhou, Maja R. Radojčić, Claire E. Ashton-James, Hanqiao Yang, Ziyi Chen, Ruijia Wang, Ying Yang, Jinhua Si, Liang Yao, Ge Li, and Lingxiao Chen

Subjects
Depression, Optimal cut-off, Covid-19, Systematic review, Psychiatry, RC435-571
Abstract

Abstract Background Studies have reported an increase in the prevalence of depression during the COVID-19 pandemic. The accuracy of screening tools may change with the prevalence and distribution of a disease in a population or sample: the “Spectrum Effect”. Methods First, we selected commonly used screening tools and developed search strategies for the inclusion of original studies during the pandemic. Second, we searched PsycINFO, EMBASE, and MEDLINE from March 2020 to September 2022 to obtain original studies that investigated the accuracy of depression screening tools during the pandemic. We then searched these databases to identify meta-analyses summarizing the accuracy of these tools conducted before the pandemic and compared the optimal cut-offs for depression screening tools during the pandemic with those before. Result Four original studies evaluating the optimal cut-offs for four screening tools (Beck Depression Inventory [BDI-II], Hospital Anxiety and Depression Scale-Depression [HADS-D], Patient Health Questionnaire-9 [PHQ-9], and Geriatric Depression Scale-4 [GDS-4]) were published during the pandemic. Four meta-analyses summarizing these tools before the pandemic. We found that the optimal cut-off of BDI-II was 14 during the pandemic (23.8% depression prevalence, screening patients with Type 2 diabetes) and 14.5 before the pandemic (17.6% depression prevalence, screening psychiatric, primary care, and healthy populations); HADS-D was 10 during the pandemic (23.8% depression prevalence, screening patients with type 2 diabetes) and 7 before the pandemic (15.0% depression prevalence, screening medically ill patients); PHQ-9 was 11 during the pandemic (14.5% depression prevalence, screening university students) and 8 before the pandemic (10.9% depression prevalence, screening the unrestricted population), and GDS-4 was 1.8 during the pandemic (29.0% depression prevalence, screening adults seen in a memory clinic setting) and 3 before the pandemic (18.5% depression prevalence, screening older adults). Conclusion The optimal cut-off for different screening tools may be sensitive to changes in study populations and reference standards. And potential spectrum effects that should be considered in post-COVID time which aiming to improve diagnostic accuracy.

Published
2023
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2. Exosomes released from PD-L1+ tumor associated macrophages promote peritoneal metastasis of epithelial ovarian cancer by up-regulating T cell lipid metabolism

Author

Jun Ma, Qianqian Cen, Qingzhu Wang, Li Liu, and Jieru Zhou

Subjects
Epithelial ovarian cancer, Tumor associated macrophages, CD8+ T cells, PD-L1, Peritoneal metastasis, Biology (General), QH301-705.5, Biochemistry, QD415-436
Abstract

Epithelial ovarian cancer (EOC) tends to metastasize to the peritoneum, and the prognosis of patients is poor. In the peritoneum of patients with EOC, TAMs (tumor associated macrophages) regulate the imbalance of T cell ratio and promote the progression and metastasis of EOC. However, the mechanism of peritoneal metastasis in EOC patients remains unclear. Here, we confirmed that the percentages of PD-L1+ TAMs in EOC tissues increased significantly, and TAMs-derived PD-L1+ exosomes affected the transcription factor PPARα to up-regulate the expression of CPT1A in CD8+ T cells, promote fatty acid oxidation, and increase reactive oxygen species to cause cell damage. The apoptosis of CD8+ T cells was increased, and the expressions of their exhaustion markers LAG3, TIM-3, and PD-1 were also up-regulated. TAMs affect T cell function through lipid metabolism, leading to peritoneal immune imbalance and promoting peritoneal metastasis of EOC. This study reveals the mechanism by which TAMs in the peritoneal microenvironment regulate T cell lipid metabolism through exosome delivery of PD-L1, and the effect of lipid metabolism on T cell function, reveals the molecular mechanism of tumor immune microenvironment affecting EOC metastasis, and further explores related pathways whether molecular blockade can be used as a means to intervene in disease progression is expected to establish a new strategy for the diagnosis and treatment of EOC.

Published
2023
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3. Stratification of diabetic kidney diseases via data-independent acquisition proteomics–based analysis of human kidney tissue specimens

Author

Qinghua Huang, Xianming Fei, Zhaoxian Zhong, Jieru Zhou, Jianguang Gong, Yuan Chen, Yiwen Li, and Xiaohong Wu

Subjects
diabetic kidney disease, progression, autophagy, tissue proteomics, identification, Diseases of the endocrine glands. Clinical endocrinology, RC648-665
Abstract

AimThe aims of this study were to analyze the proteomic differences in renal tissues from patients with diabetes mellitus (DM) and diabetic kidney disease (DKD) and to select sensitive biomarkers for early identification of DKD progression.MethodsPressure cycling technology–pulse data-independent acquisition mass spectrometry was employed to investigate protein alterations in 36 formalin-fixed paraffin-embedded specimens. Then, bioinformatics analysis was performed to identify important signaling pathways and key molecules. Finally, the target proteins were validated in 60 blood and 30 urine samples.ResultsA total of 52 up- and 311 down-regulated differential proteins were identified as differing among the advanced DKD samples, early DKD samples, and DM controls (adjusted p

Published
2022
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4. A circulating miR-19b-based model in diagnosis of human breast cancer

Author

Qian Zhao, Lei Shen, Jinhui Lü, Heying Xie, Danni Li, Yuanyuan Shang, Liqun Huang, Lingyu Meng, Xuefeng An, Jieru Zhou, Jing Han, and Zuoren Yu

Subjects
breast cancer, circulating miRNA, biomarker, diagnosis model, miR-19, Biology (General), QH301-705.5
Abstract

Abstract Objective: Breast cancer (BC) is becoming the leading cause of cancer-related death in women all over the word. Identification of diagnostic biomarkers for early detection of BC is one of the most effective ways to reduce the mortality.Methods: Plasma samples from BC patients (n = 120) and normal controls (n = 50) were collected to determine the differentially expressed circulating miRNAs in BC patients. Binary logistic regression was applied to develop miRNA diagnostic models. Receiver operating characteristic (ROC) curves were applied to calculate the area under the curve (AUC). MMTV-PYMT mammary tumor mice were used to validate the expression change of those circulating miRNAs. Plasma samples from patients with other tumor types were collected to determine the specificity of the model in diagnosis of BC.Results: In the screening phase, 5 circulating miRNAs (miR-16, miR-17, miR-19b, miR-27a, and miR-106a) were identified as the most significantly upregulated miRNAs in plasma of BC patients. In consistence, the 5 miRNAs showed upregulation in the circulation of additional 80 BC patients in a tumor stage-dependent manner. Application of a tumor-burden mice model further confirmed upregulation of the 5 miRNAs in circulation. Based on these data, five models with diagnostic potential of BC were developed. Among the 5 miRNAs, miR-19b ranked at the top position with the highest specificity and the biggest contribution. In combination with miR-16 and miR-106a, a miR-19b-based 3-circulating miRNA model was selected as the best for further validation. Taken the samples together, the model showed 92% of sensitivity and 90% of specificity in diagnosis of BC. In addition, three other tumor types including prostate cancer, thyroid cancer and colorectal cancer further verified the specificity of the BC diagnostic model. Conclusion: The current study developed a miR-19b-based 3-miRNA model holding potential for diagnosis of BC using blood samples.

Published
2022
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5. PDE-5-Inhibited BMSCs Alleviate High Glucose-Induced Myocardial Fibrosis and Cardiomyocyte Apoptosis by Activating the cGMP/PKG Pathway

Author

Qinghua Huang, Jiangbo Ma, Hui Wu, Jieru Zhou, Lei Jiang, and Xianming Fei

Subjects
phosphodiesterase-5, bmscs, diabetic cardiomyopathy, cgmp/pkg pathway, myocardial fibrosis, cardiomyocytes apoptosis, Biochemistry, QD415-436, Biology (General), QH301-705.5
Abstract

Background: Phosphodiesterase-5 (PDE-5) inhibitors have been found to play an important cardio-protective role. This study aimed to clarify the inhibitory effects of PDE-5-silenced bone marrow mesenchymal stem cells (BMSCs) on high glucose-induced myocardial fibrosis and cardiomyocyte apoptosis. Methods: Cardiomyocytes and fibroblasts of neonatal rats were treated with high glucose (HG), and co-cultured with PDE-5-overexpressed or -knocked down BMSCs. The viability and apoptosis as well as the levels of cytokines, Cardiac troponin I and Vimentin of cardiomyocytes and fibroblasts were studied. The expressions of PDE-5, cyclic guanosine monophosphate (cGMP) and protein kinase G (PKG), in both cells were evaluated. Results: BMSCs that silenced PDE-5 facilitated the viability of cardiomyocytes, decreased the viability of fibroblasts, and inhibited the apoptosis of cardiomyocytes and fibroblasts. The contents of collagen-I, collagen-III, tissue inhibitor of metalloproteinase (TIMP)-1 and Dermin in fibroblasts were decreased by the PDE-5 inhibitor, but the levels of matrix metalloproteinase (MMP)-1 in fibroblasts and troponin-I in cardiomyocytes were increased by the PDE-5 inhibitor. PDE-5 inhibitor also suppressed the expression of PDE-5 but up-regulated cGMP and PKG expression in cardiomyocytes and fibroblasts. Conclusions: PDE-5-inhibited BMSCs can decrease HG-induced myocardial fibrosis and cardiomyocyte apoptosis by activating the cGMP/PKG pathway, and may play a role in the prevention and treatment of diabetic cardiomyopathy.

Published
2023
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6. The high platelet counts as predictor for early foetal demise

Author

Xiaowen Shao, Dandan Wang, Yue Xu, Ling Guo, Hui Yang, Jieru Zhou, Jiayi Liang, Jie Qian, Jiajing Cheng, Lihua Sun, and Yaozu Xiang

Subjects
Pregnancy, early foetal demise, platelet counts, mean platelet volume, Medicine
Abstract

AbstractObjectives Early fetal demise (absence of cardiac activity in a visible fetus) is a very common event, but there are no reliable biomarkers to predict it. The purpose of the study was to assess the association of platelet parameters with early fetal demise.Methods In this case-control study, we included women with normal deliveries or those ultrasound diagnosed as early fetal demise. For those who were identified with early fetal demise, the platelet parameters were analyzed before the ultrasound diagnosis, which is based on the absence of either an embryo within a gestational sac or cardiac activity in a visible embryo in the 5-10 weeks of gestation. The association between the risk of early fetal demise with the women's mean platelet volume (MPV) and platelet counts was calculated by logistic regression. Duplicate measurements of platelet aggregation were performed with VerifyNow.Results In total, 99 women identified with early fetal demise and 170 women who had an uncomplicated pregnancy with normal delivery from January 2017 and August 2020 were included in the study. We found that platelet counts in the early fetal demise group were significantly higher than healthy pregnancies. In addition, platelet reactivity is higher in the normal delivery group than those in early fetal demise group (p < .05). High levels of platelet counts resulted in an adjusted odds ratio (OR) of 2.075 (95% confidence interval [95% CI], 1.215–3.544; p = .008) for early fetal demise.Conclusions Increased platelet counts in the first trimester may be a predictor for the risk of early fetal demise.

Published
2021
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7. MicroRNA Variants and HLA-miRNA Interactions are Novel Rheumatoid Arthritis Susceptibility Factors

Author

Shicheng Guo, Yehua Jin, Jieru Zhou, Qi Zhu, Ting Jiang, Yanqin Bian, Runrun Zhang, Cen Chang, Lingxia Xu, Jie Shen, Xinchun Zheng, Yi Shen, Yingying Qin, Jihong Chen, Xiaorong Tang, Peng Cheng, Qin Ding, Yuanyuan Zhang, Jia Liu, Qingqing Cheng, Mengru Guo, Zhaoyi Liu, Weifang Qiu, Yi Qian, Yang Sun, Yu Shen, Hong Nie, Steven J. Schrodi, and Dongyi He

Subjects
rheumatoid arthritis, miRNA, SNP, susceptibility, predispose, Genetics, QH426-470
Abstract

Genome-wide association studies have identified >100 genetic risk factors for rheumatoid arthritis. However, the reported genetic variants could only explain less than 40% heritability of rheumatoid arthritis. The majority of the heritability is still missing and needs to be identified with more studies with different approaches and populations. In order to identify novel function SNPs to explain missing heritability and reveal novel mechanism pathogenesis of rheumatoid arthritis, 4 HLA SNPs (HLA-DRB1, HLA-DRB9, HLA-DQB1, and TNFAIP3) and 225 common SNPs located in miRNA, which might influence the miRNA target binding or pre-miRNA stability, were genotyped in 1,607 rheumatoid arthritis and 1,580 matched normal individuals. We identified 2 novel SNPs as significantly associated with rheumatoid arthritis including rs1414273 (miR-548ac, OR = 0.84, p = 8.26 × 10−4) and rs2620381 (miR-627, OR = 0.77, p = 2.55 × 10−3). We also identified that rs5997893 (miR-3928) showed significant epistasis effect with rs4947332 (HLA-DRB1, OR = 4.23, p = 0.04) and rs2967897 (miR-5695) with rs7752903 (TNFAIP3, OR = 4.43, p = 0.03). In addition, we found that individuals who carried 8 risk alleles showed 15.38 (95%CI: 4.69–50.49, p < 1.0 × 10−6) times more risk of being affected by RA. Finally, we demonstrated that the targets of the significant miRNAs showed enrichment in immune related genes (p = 2.0 × 10−5) and FDA approved drug target genes (p = 0.014). Overall, 6 novel miRNA SNPs including rs1414273 (miR-548ac, p = 8.26 × 10−4), rs2620381 (miR-627, p = 2.55 × 10−3), rs4285314 (miR-3135b, p = 1.10 × 10−13), rs28477407 (miR-4308, p = 3.44 × 10−5), rs5997893 (miR-3928, p = 5.9 × 10−3) and rs45596840 (miR-4482, p = 6.6 × 10−3) were confirmed to be significantly associated with RA in a Chinese population. Our study suggests that miRNAs might be interesting targets to accelerate understanding of the pathogenesis and drug development for rheumatoid arthritis.

Published
2021
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10. Data from Exosomes Released from Tumor-Associated Macrophages Transfer miRNAs That Induce a Treg/Th17 Cell Imbalance in Epithelial Ovarian Cancer

Author

Xipeng Wang, Yingying Lin, Kai Wang, Husheng Wang, Xinjing Wang, Qinyi Zhu, Ting Zhang, Xiaoli Wu, Xiaoduan Li, and Jieru Zhou

Abstract

The immune microenvironment is crucial for epithelial ovarian cancer (EOC) progression and consists of tumor-associated macrophages (TAM) and T lymphocytes, such as regulatory T cells (Treg) and T helper 17 (Th17) cells. In this study, the Treg/Th17 ratio was significantly higher in EOC in situ and in metastatic peritoneal tissues than in benign ovarian tumors and benign peritoneum. The Treg/Th17 ratio was associated with histologic grade and was an independent prognostic factor for overall survival of EOC patients. On the basis of microarray analysis of exosomes derived from TAMs, we identified miRNAs enriched in the exosomes, including miR-29a-3p and miR-21-5p. When the two miRNA mimics were transfected into CD4+ T cells, they directly suppressed STAT3 and regulated Treg/Th17 cells, inducing an imbalance, and they had a synergistic effect on STAT3 inhibition. Taken together, these results indicate that exosomes mediate the interaction between TAMs and T cells, generating an immune-suppressive microenvironment that facilitates EOC progression and metastasis. These findings suggest that targeting these exosomes or their associated miRNAs might pave the way for the development of novel treatments for EOC.

Published
2023

11. Supplementary Tables from Exosomes Released from Tumor-Associated Macrophages Transfer miRNAs That Induce a Treg/Th17 Cell Imbalance in Epithelial Ovarian Cancer

Author

Xipeng Wang, Yingying Lin, Kai Wang, Husheng Wang, Xinjing Wang, Qinyi Zhu, Ting Zhang, Xiaoli Wu, Xiaoduan Li, and Jieru Zhou

Abstract

2. This file contains supplemental table S1-S5. It shows univariate/multivariable analysis of OS in EOC patients, the absolute counts of Treg/Th17 cells in flow cytometric analysis, and the proportion of cytokines from Tregs/Th17 cells in flow cytometric analysis.

Published
2023

13. Urinary N‐acetyl‐β‐ <scp>d</scp> ‐glucosaminidase‐creatine ratio is a valuable predictor for advanced diabetic kidney disease

Author

Qinghua, Huang, Xianming, Fei, Huifang, Zhan, Jianguang, Gong, Jieru, Zhou, Yulv, Zhang, Xiao, Ye, Yingxiang, Song, Jiangbo, Ma, and Xiaohong, Wu

Subjects
Microbiology (medical), Medical Laboratory Technology, Biochemistry (medical), Clinical Biochemistry, Public Health, Environmental and Occupational Health, Immunology and Allergy, Hematology
Abstract

Many biomarkers show high diagnostic values for diabetic kidney disease (DKD), but fewer studies focus on the predictive assessment of DKD progression by blood and urinary biomarkers.This study aims to find powerful risk predictors and identifying biomarkers in blood and urine for DKD progression.A total of 117 patients with type 2 DKD including early and advanced stages and their laboratory parameters were statistically assessed. A receiver operating characteristic (ROC) curve analysis was performed to evaluate the significance of discriminating between early and advanced DKD, and the predictive power for advanced DKD was analyzed by regression analysis and trisector grouping.N-acetyl-β-d-glucosaminidase-creatine (NAG/CR) level in advanced DKD was statistically higher than that in early DKD (p 0.05), and there was a higher incidence of advanced DKD (72% vs. 56%) and high odds ratio (OR: 3.917, 95% CI: 1.579-10.011) of NAG/CR with ≥2.79 U/mmol compared with2.79 U/mmol (p 0.05). NAG/CR ratio also showed a higher area under the ROC curve of 0.727 (95% CI: 0.616-0.828, p = 0.010) with a high sensitivity (0.75) and a moderate specificity (0.66) when 1.93 U/mmol was set as the optimal cutoff value. The adjusted-multivariable analysis revealed that NAG/CR had an OR of 1.021 (95% CI: 1.024-1.038) and 2.223 (95% CI: 1.231-4.463) based on a continuous and categorical variable, respectively, for risk of advanced DKD. Moreover, the prevalence of advanced DKD exhibited an increasing tendency by an increment of the trisector of NAG/CR.This study suggests that NAG/CR ratio is an independent predictor for advanced DKD, and it also can be used as a powerful identifying marker between early and advanced DKD.

Published
2022

14. NOL4L, a novel nuclear protein, promotes cell proliferation and metastasis by enhancing the PI3K/AKT pathway in ovarian cancer

Author

Feikai Lin, Xiaoduan Li, Jieru Zhou, and Xipeng Wang

Subjects
0301 basic medicine, Biophysics, Mice, Nude, Biochemistry, Metastasis, 03 medical and health sciences, Phosphatidylinositol 3-Kinases, 0302 clinical medicine, Nude mouse, Neuroblastoma, Cell Line, Tumor, medicine, Animals, Humans, Neoplasm Invasiveness, Molecular Biology, PI3K/AKT/mTOR pathway, Cell Proliferation, Ovarian Neoplasms, biology, Cell growth, Proteins, Cell Biology, biology.organism_classification, medicine.disease, 030104 developmental biology, Tumor progression, 030220 oncology & carcinogenesis, Cancer research, Immunohistochemistry, Female, Ovarian cancer, Proto-Oncogene Proteins c-akt, Signal Transduction
Abstract

Nucleolar protein 4-like (NOL4L) was first identified in acute myeloid leukaemia. Then, it was verified to be involved in cell progression in neuroblastoma. However, the functional role of NOL4L in tumor proliferation and metastasis and the underlying molecular mechanism(s) are not fully understood. Immunohistochemistry (IHC) assays were performed in patient tissues to reveal NOL4L expression profiles. Then, we knocked down NOL4L in two ovarian cancer cell lines (Skov3-ip1 and Hey), and cell-based in-vitro and in-vivo assays were subsequently conducted to gain insight into the underlying mechanism of NOL4L in ovarian cancer. We confirmed that the expression of NOL4L was higher in tumor tissues, especially in peritoneal metastatic tissues. Furthermore, we observed that NOL4L was related to prognosis in ovarian cancer patients. Next, we conducted CCK-8 assays, colony formation assays, migration and invasion experiments and wound healing assays and verified that NOL4L could promote proliferation and metastasis in ovarian cancer cells. In addition, NOL4L promoted tumor progression and metastasis in a nude mouse model. Mechanistically, we demonstrated that NOL4L influenced gene expression in the PI3K/AKT pathway. Overall, our study provides genetic and biochemical evidence that NOL4L is critical for tumor progression and metastasis in ovarian cancer cells. Thus, it could serve as a target for antimetastatic therapy in ovarian cancer.

Published
2021

15. Understanding the Blockchain Ecosystem with Analysis of Decentralized Applications: an Empirical Study

Author

Yong Yan, Cui Weiping, Jieru Zhou, Yixin Sun, Ying Wang, and Lei Huang

Subjects
Blockchain, Process management, Empirical research, Traceability, Computer science, Decentralization, Tamper resistance
Abstract

Blockchain technology is characterized by decentralization, traceability and tamper resistance. A decentralized application (Dapp) refers to a blockchain-based application deployed on Ethereum platform, with the aim to facilitate decentralized smart contracts and to prosper the blockchain ecosystem. In this paper, an in-depth analysis for the current decentralized applications is performed to reveal the structure and development situation of blockchain ecosystem. With a better understanding of the blockchain ecosystem, insights can be obtained for the Dapp developers as well as for the potential blockchain adopters. Furthermore, guidelines and suggestions are provided to inspire the business applications based on blockchain.

Published
2021

16. Exosomes derived from hypoxic epithelial ovarian cancer cells deliver microRNAs to macrophages and elicit a tumor-promoted phenotype

Author

Xipeng Wang, Xiaoduan Li, Xinjing Wang, Yingying Lin, Jieru Zhou, and Xin Chen

Subjects
0301 basic medicine, Cancer Research, Stromal cell, endocrine system diseases, Transplantation, Heterologous, Mice, Nude, Carcinoma, Ovarian Epithelial, Biology, Exosomes, 03 medical and health sciences, 0302 clinical medicine, Cell Movement, Cell Line, Tumor, microRNA, Tumor Microenvironment, medicine, Animals, Humans, Hypoxia, Cell Proliferation, Ovarian Neoplasms, Tumor microenvironment, Microarray analysis techniques, Cell growth, Macrophages, Cancer, medicine.disease, Survival Analysis, Cell Hypoxia, female genital diseases and pregnancy complications, Microvesicles, Gene Expression Regulation, Neoplastic, MicroRNAs, Cell Transformation, Neoplastic, Phenotype, 030104 developmental biology, Oncology, 030220 oncology & carcinogenesis, Cancer cell, Cancer research, Female
Abstract

Recently, cancer has been considered to be a complex system that includes the tumor microenvironment (TME). Tumor-associated macrophages (TAMs) are the most common immune-related stromal cells in the TME, and communication between cancer cells and TAMs is crucial for the progression of epithelial ovarian cancer (EOC). In this study, we revealed that exosomes derived from EOC cells remodel macrophages to a tumor-promoted phenotype, namely TAMs. In addition, hypoxic microenvironments have been postulated to facilitate this process in the TME, and hypoxia-inducible factors (HIFs) play an important role in this process. We found that TAMs educated by hypoxic exosomes derived from EOC cells promote tumor proliferation and migration in a feedback loop. Based on microarray analysis of normoxic and hypoxic exosomes, we discovered that a panel of miRNAs was enriched in hypoxic exosomes. And these three highly expressed miRNAs were induced by hypoxia via HIFs. In this study, we revealed that under hypoxic conditions, EOC cell-derived exosomes deliver miRNAs to induce M2 macrophage polarization, which promotes EOC cell proliferation and migration. This study suggests that these exosomes and associated miRNAs might serve as targets for novel treatments or diagnostic biomarkers for EOC.

Published
2018

17. Triptolide decreases rheumatoid arthritis fibroblast-like synoviocyte proliferation, invasion, inflammation and presents a therapeutic effect in collagen-induced arthritis rats via inactivating lncRNA RP11-83J16.1 mediated URI1 and β-catenin signaling

Author

Xuemei Piao, Luan Xue, and Jieru Zhou

Subjects
Male, musculoskeletal diseases, Fibroblast-like synoviocyte, Primary Cell Culture, Immunology, Cell, Arthritis, Inflammation, Arthritis, Rheumatoid, chemistry.chemical_compound, Cell Movement, In vivo, medicine, Animals, Humans, Immunology and Allergy, Cells, Cultured, beta Catenin, Cell Proliferation, Pharmacology, Synovial Membrane, Fibroblasts, Phenanthrenes, Triptolide, medicine.disease, Arthritis, Experimental, Synoviocytes, eye diseases, In vitro, Rats, Repressor Proteins, medicine.anatomical_structure, chemistry, Apoptosis, Cancer research, Epoxy Compounds, RNA, Long Noncoding, Diterpenes, medicine.symptom, Signal Transduction
Abstract

Objective Our previous study observed that long non-coding RNA (lncRNA) RP11-83J16.1 promoted rheumatoid arthritis (RA)-fibroblast-like synoviocyte (RA-FLS) proliferation, invasion and inflammation, which was downregulated by triptolide treatment. Therefore, the present study aimed to further investigate the mechanism and interaction between triptolide and lncRNA RP11-83J16.1 in RA treatment in vitro and in vivo. Methods RA-FLS was isolated and treated by different concentration of triptolide and lncRNA RP11-83J16.1 overexpression plasmid. Furthermore, collagen-induced arthritis (CIA) rat model was constructed followed by triptolide and lncRNA RP11-83J16.1 overexpression plasmid treatment. Results Triptolide inhibited RA-FLS viability and lncRNA RP11-83J16.1 expression in a dose-dependent manner. Afterward, triptolide treatment inhibited RA-FLS proliferation, invasion, levels of inflammatory markers (TNF-α, IL-1β, IL-6, MMP-3, and MMP-9), inactivated lncRNA RP11-83J16.1, URI1 and β-catenin signaling, but promoted apoptosis. However, lncRNA RP11-83J16.1 overexpression weakened the effects of triptolide on regulating RA-FLS cell behaviors, URI1 signaling and β-catenin signaling. In CIA model, triptolide decreased arthritis score, hyperproliferation of synovial cells, inflammation infiltration of synovial tissue, inflammatory markers (TNF-α, IL-1β, IL-6, MMP-3, and MMP-9), inactivated lncRNA RP11-83J16.1, URI1 and β-catenin signaling, but increased cell apoptosis rate of synovial tissue. Nevertheless, lncRNA RP11-83J16.1 curtailed the treatment effect of triptolide in CIA model. Conclusion Triptolide decreases RA-FLS proliferation, invasion, inflammation and presents a therapeutic effect in CIA model via inactivating lncRNA RP11-83J16.1 mediated URI1 and β-catenin signaling.

Published
2021

18. The Treatment of Cesarean Scar Pregnancy with Uterine Artery Embolization and Curettage as Compared to Transvaginal Hysterotomy

Author

Xipeng Wang, Jieru Zhou, Huihui Chen, Weilin Tan, Min Yao, and Husheng Wang

Subjects
Adult, medicine.medical_specialty, medicine.medical_treatment, Cesarean Scar Pregnancy, Menstrual status, Dilatation and Curettage, Menstruation, 03 medical and health sciences, 0302 clinical medicine, Uterine artery embolization, Pregnancy, medicine, Humans, 030212 general & internal medicine, Hysterotomy, Retrospective Studies, 030219 obstetrics & reproductive medicine, Cesarean Section, Obstetrics, business.industry, Obstetrics and Gynecology, Retrospective cohort study, Uterine Artery Embolization, Curettage, Pregnancy, Ectopic, Surgery, Treatment Outcome, Reproductive Medicine, Female, Complication, business
Abstract

To investigate the outcome and menstrual status in patients after treatment of cesarean scar pregnancy (CSP) by transvaginal hysterotomy or uterine artery embolization combined with uterine curettage.A retrospective cohort study. An analysis of CSP patients was performed using records from Shanghai First MaternityInfant Hospital affiliated with Tongji University for the period between July 16, 2014 and January 22, 2016. Twenty-seven patients were treated with transvaginal hysterotomy and in this group, 49 patients received uterine curettage after UAE. The clinical information on these patients and clinical outcomes especially the status of menstruation were reviewed.There was only one complication in transvaginal hysterotomy group, while 3 cases of villus residue occurred in UAE group. Nineteen patients (70.4%) in transvaginal hysterotomy group self-assessed their menstrual volumes, which had no remarkable changes; 6 patients (22.2%) felt that their menstrual volumes had decreased. Thirty-five patients in UAE group (71.4%) reported that their menstrual volumes decreased (P0.05). The range of pictorial blood loss score was 55-82 in transvaginal hysterotomy group and 9-74 in UAE group, and the mean pictorial blood loss score was decreased from 68.4 to 65.8 in transvaginal hysterotomy group (a 3.2±4.4% reduction) and from 66.4 to 38.8 in UAE group (a 41.7±26.4% reduction) (P0.05).Transvaginal hysterotomy appears to be more advantageous than UAE combined with uterine curettage. The menstrual interval and duration changed significantly in UAE group.

Published
2017

19. Cesarean section scar diverticulum evaluation by saline contrast-enhanced magnetic resonance imaging: The relationship between variable parameters and longer menstrual bleeding

Author

Xipeng Wang, Pin Chen, Minghua Sun, Jieru Zhou, Jialiang Zhu, Wenjing Wang, and Min Yao

Subjects
medicine.medical_specialty, 030219 obstetrics & reproductive medicine, medicine.diagnostic_test, business.industry, medicine.medical_treatment, Obstetrics and Gynecology, Magnetic resonance imaging, medicine.disease, Imaging modalities, 03 medical and health sciences, 0302 clinical medicine, Menstrual bleeding, Transvaginal ultrasound, Correlation analysis, Medicine, 030212 general & internal medicine, Radiology, business, Saline, Contrast-enhanced Magnetic Resonance Imaging, Diverticulum
Abstract

Aim This study was conducted to determine a more accurate imaging method for the diagnosis of cesarean scar diverticulum (CSD) and to identify the parameters of CSD strongly associated with prolonged menstrual bleeding. Methods We enrolled 282 women with a history of cesarean section (CS) who presented with prolonged menstrual bleeding between January 2012 and May 2015. Transvaginal ultrasound, general magnetic resonance imaging (MRI) and contrast-enhanced MRI were used to diagnose CSD. Five parameters were compared among the imaging modalities: length, width, depth and thickness of the remaining muscular layer (TRM) of CSD and the depth/TRM ratio. Correlation between the five parameters and days of menstrual bleeding was performed. Finally, multivariate analysis was used to determine the parameters associated with menstrual bleeding longer than 14 days. Results Contrast-enhanced MRI yielded greater length or width or thinner TRM of CSD compared with MRI and transvaginal ultrasound. CSD size did not significantly differ between women who had undergone one and two CSs. Correlation analysis revealed that CSD (P = 0.038) and TRM (P = 0.003) lengths were significantly associated with days of menstrual bleeding. Longer than 14 days of bleeding was defined by cut-off values of 2.15 mm for TRM and 13.85 mm for length. TRM and number of CSs were strongly associated with menstrual bleeding longer than 14 days. Conclusions CE-MRI is a relatively accurate and efficient imaging method for the diagnosis of CSD. A cut-off value of TRM of 2.15 mm is the most important parameter associated with menstrual bleeding longer than 14 days.

Published
2017

20. Role of RP11-83J16.1, a novel long non-coding RNA, in rheumatoid arthritis

Author

Xuemei, Piao, Jieru, Zhou, and Jiandong, Hu

Subjects
Original Article
Abstract

This study aimed to explore the effects of long non-coding RNA (lncRNA) expression on rheumatoid arthritis (RA). LncRNA expression profiles were obtained from the synovial tissues of five RA patients and five age-/gender-matched controls by RNA-Seq. Six candidate lncRNAs were then chosen and their levels in synovial fluid further examined in 25 RA patients and 25 health controls using RT-qPCR. The effects of lncRNA RP11-83J16.1 overexpression and knockdown on RA fibroblast-like synoviocytes (RA-FLS) function, inflammation state, and URI1, FRAT1, and β-catenin levels were assessed. After RNA-Seq, lncRNA expression profiles clearly distinguished RA patients from controls, and 190 upregulated lncRNAs and 131 downregulated lncRNAs were identified, which were mainly enriched in proliferative/immune/inflammatory pathways. Results of RT-qPCR showed that the levels of lncRNAs MTCO2P12, KCNQ5-IT1 and RP11-83J16.1 were increased, whereas lncRNAs LINC00570, RP11-342M1.6, and REXO1L4P were decreased in RA patients compared to controls. Notably, lncRNA RP11-83J16.1 correlated with increased inflammation and disease activity in RA patients. Additionally, lncRNA RP11-83J16.1 promoted cell proliferation, migration, invasion and inflammation, reduced apoptosis, and positively regulates cellular URI1, FRAT1 and β-catenin expression in RA-FLS. Rescue experiments revealed that URI1 overexpression compensated for the regulatory effects of lncRNA RP11-83J16.1 knockdown in RA-FLS. In conclusion, lncRNA RP11-83J16.1, a novel lncRNA identified by RNA-Seq, correlates with increased risk and disease activity of RA, and promotes RA-FLS proliferation, migration, invasion and inflammation by regulating URI1 and downstream β-catenin pathway components.

Published
2019

21. Defect width: the prognostic index for vaginal repair of cesarean section diverticula

Author

Weilin Tan, Xipeng Wang, Jieru Zhou, Min Yao, Xingchen Zhou, and Husheng Wang

Subjects
Adult, Vaginal approach, medicine.medical_specialty, Cohort Studies, Menstruation, 03 medical and health sciences, Gynecologic Surgical Procedures, 0302 clinical medicine, Suture (anatomy), Pregnancy, medicine, Humans, 030212 general & internal medicine, Wound Healing, 030219 obstetrics & reproductive medicine, Cesarean Section, business.industry, Obstetrics and Gynecology, General Medicine, Prognosis, Vaginal repair, Surgery, Diverticulum, Transvaginal ultrasound, Vagina, Female, business, Cohort study
Abstract

To evaluate the clinical parameter associated with cesarean section diverticula anatomic healing via vaginal repair management.Observational cohort study. From Jul 2014 to Dec 2015, 143 women with CSD underwent vaginal repair surgery in Shanghai First Maternity and Infant Hospital, and 137(95.80%) were diagnosed using both transvaginal ultrasound and MRI. A total of 124 patients (86.71%) who were followed-up for more than 6 months after surgery were enrolled in this study. Excision and suture of CSD was performed through the vaginal approach. The defect sizes of the width, length, depth and TRM before or after repair were evaluated.The mean preoperative duration of menstruation was 14.47 ± 3.30 days and the thickness of the remaining muscular layer was 2.65 ± 1.13 mm before surgery. The study revealed that the healing effects of CSD repair stabilized 3 months after surgery. At the median follow-up time (11.28 months), CSD disappeared after surgery in 64.52% of patients (80/124), and 60.0% of patients (48/80) reached ≤7 days of menstruation. Meanwhile, for 35.48% of patients (44/124), CSD persisted at the median follow-up after surgery, and 31.82% (14/44) of these patients reached ≤7 days of menstruation(P 0.05). TRM at a median follow-up time after vaginal repair7.88 mm, 92.11% (70/76) of CSD disappeared. Moreover, when preoperative CSD width ≤18.85 mm indicates that only 18.75% (12/64) of patients will present with CSD after vaginal repair, as determined by MRI (95% CI 0.515-0.737).The defect width of the preoperative CSD was the prognostic index of CSD anatomical repair effect. When the preoperative CSD width18.85 mm, we should pay more attention to the edge of the defect during vaginal repairing.

Published
2016

22. Institutional constraints of product innovation in China: Evidence from international joint ventures

Author

Zhenzhong Ma, Jieru Zhou, Mingyang Yu, Chongyan Gao, and Zhenning Yang

Subjects
Marketing, Government, business.industry, Product innovation, Context (language use), International trade, Investment (macroeconomics), ComputingMilieux_GENERAL, New product development, International joint venture, Product (category theory), business, China, Industrial organization
Abstract

Research interests in product innovation and relevant success factors in an international context are increasing in the past decades. With the data from over 400 international joint ventures in China's manufacturing sector, this study explores China's institutional factors that may significantly affect product innovations in international joint ventures. The results show that while total R&D investment can increase China's international joint ventures' global product sales brought by new product innovations, local professional services and infrastructure including local universities and research institutes in China can also facilitate product innovation and patent applications. In addition, local market for talents and technologies in China has negative impact on international joint ventures' efforts to sell products overseas. This paper concludes with the discussions of the implications for Chinese government and international joint ventures in China's market.

Published
2015

23. Exosomes Released from Tumor-Associated Macrophages Transfer miRNAs That Induce a Treg/Th17 Cell Imbalance in Epithelial Ovarian Cancer

Author

Jieru Zhou, Xiaoli Wu, Xinjing Wang, Xipeng Wang, Xiaoduan Li, Ting Zhang, Yingying Lin, Qinyi Zhu, H. W. Wang, and Kai Wang

Subjects
0301 basic medicine, CD4-Positive T-Lymphocytes, STAT3 Transcription Factor, Cancer Research, endocrine system diseases, Immunology, Cell, chemical and pharmacologic phenomena, Carcinoma, Ovarian Epithelial, Exosomes, T-Lymphocytes, Regulatory, Metastasis, 03 medical and health sciences, Cell Line, Tumor, microRNA, medicine, Tumor Microenvironment, Animals, Humans, STAT3, Ovarian Neoplasms, Tumor microenvironment, biology, Microarray analysis techniques, Macrophages, hemic and immune systems, Transfection, Middle Aged, medicine.disease, female genital diseases and pregnancy complications, Microvesicles, Mice, Inbred C57BL, MicroRNAs, 030104 developmental biology, medicine.anatomical_structure, biology.protein, Cancer research, Th17 Cells, Female
Abstract

The immune microenvironment is crucial for epithelial ovarian cancer (EOC) progression and consists of tumor-associated macrophages (TAM) and T lymphocytes, such as regulatory T cells (Treg) and T helper 17 (Th17) cells. In this study, the Treg/Th17 ratio was significantly higher in EOC in situ and in metastatic peritoneal tissues than in benign ovarian tumors and benign peritoneum. The Treg/Th17 ratio was associated with histologic grade and was an independent prognostic factor for overall survival of EOC patients. On the basis of microarray analysis of exosomes derived from TAMs, we identified miRNAs enriched in the exosomes, including miR-29a-3p and miR-21-5p. When the two miRNA mimics were transfected into CD4+ T cells, they directly suppressed STAT3 and regulated Treg/Th17 cells, inducing an imbalance, and they had a synergistic effect on STAT3 inhibition. Taken together, these results indicate that exosomes mediate the interaction between TAMs and T cells, generating an immune-suppressive microenvironment that facilitates EOC progression and metastasis. These findings suggest that targeting these exosomes or their associated miRNAs might pave the way for the development of novel treatments for EOC.

Published
2017

24. Cesarean section scar diverticulum evaluation by saline contrast-enhanced magnetic resonance imaging: The relationship between variable parameters and longer menstrual bleeding

Author

Min, Yao, Wenjing, Wang, Jieru, Zhou, Minghua, Sun, Jialiang, Zhu, Pin, Chen, and Xipeng, Wang

Subjects
Adult, Cicatrix, Diverticulum, Cesarean Section, Uterus, Humans, Female, Sodium Chloride, Image Enhancement, Magnetic Resonance Imaging, Menorrhagia
Abstract

This study was conducted to determine a more accurate imaging method for the diagnosis of cesarean scar diverticulum (CSD) and to identify the parameters of CSD strongly associated with prolonged menstrual bleeding.We enrolled 282 women with a history of cesarean section (CS) who presented with prolonged menstrual bleeding between January 2012 and May 2015. Transvaginal ultrasound, general magnetic resonance imaging (MRI) and contrast-enhanced MRI were used to diagnose CSD. Five parameters were compared among the imaging modalities: length, width, depth and thickness of the remaining muscular layer (TRM) of CSD and the depth/TRM ratio. Correlation between the five parameters and days of menstrual bleeding was performed. Finally, multivariate analysis was used to determine the parameters associated with menstrual bleeding longer than 14 days.Contrast-enhanced MRI yielded greater length or width or thinner TRM of CSD compared with MRI and transvaginal ultrasound. CSD size did not significantly differ between women who had undergone one and two CSs. Correlation analysis revealed that CSD (P = 0.038) and TRM (P = 0.003) lengths were significantly associated with days of menstrual bleeding. Longer than 14 days of bleeding was defined by cut-off values of 2.15 mm for TRM and 13.85 mm for length. TRM and number of CSs were strongly associated with menstrual bleeding longer than 14 days.CE-MRI is a relatively accurate and efficient imaging method for the diagnosis of CSD. A cut-off value of TRM of 2.15 mm is the most important parameter associated with menstrual bleeding longer than 14 days.

Published
2016

25. Vaginal Repair of Cesarean Section Scar Diverticula that Resulted in Improved Postoperative Menstruation

Author

Weilin Tan, Xipeng Wang, Min Yao, Pin Chen, Husheng Wang, and Jieru Zhou

Subjects
Adult, medicine.medical_specialty, Metrorrhagia, Vaginal Diseases, Scars, Pelvic Pain, Menstruation, 03 medical and health sciences, Cicatrix, 0302 clinical medicine, Suture (anatomy), Uterine Rupture, Pregnancy, medicine, Humans, Retrospective Studies, Ultrasonography, Wound Healing, 030219 obstetrics & reproductive medicine, business.industry, Task force, Cesarean Section, Pelvic pain, Obstetrics and Gynecology, Retrospective cohort study, Vaginal repair, medicine.disease, Surgery, Uterine rupture, Diverticulum, 030220 oncology & carcinogenesis, Vagina, Female, medicine.symptom, business
Abstract

Owing to the increase in cesarean sections (C-sections) worldwide, long-term complications such as postmenstrual spotting, chronic pelvic pain, and C-section scar ectopic pregnancies have created a new medical era of gynecologic disease. A new type of vaginal repair is evaluated to repair C-section diverticulum (CSD) and rebuild the muscular layer to improve symptoms of abnormal uterine bleeding and decrease the risk of uterine rupture.Retrospective cohort study (Canadian Task Force classification II-2).University hospital.A total of 121 patients with CSD diagnoses by transvaginal ultrasound (TVU) presented with postmenstrual spotting between June 2012 and March 2015. All patients had undergone at least 1 C-section delivery and had no history of postmenstrual spotting before undergoing C-section.Vaginal excision and suture of CSD.The mean duration of menstruation was 14.87 ± 3.46 days preoperatively and decreased to 8.22 ± 2.73 days at 1 month after surgery, 8.89 ± 2.67 days at 3 months after surgery, and 9.02 ± 2.47 days at 6 months after surgery (p .01). The length, width, depth, and thickness of the remaining muscular layer (TRM) at 1 month, 3 months, and 6 months assessed by TVU also improved significantly after surgery (p .05). However, postoperative menstruation and imaging data did not differ markedly between 3 months and 6 months, suggesting that follow-up at 3 months represents an adequate endpoint for evaluating the effectiveness of surgery. At 6 months, 80.3% of patients (94 of 117) reached ≤10 days of menstruation. Further study revealed that a TRM at 6 months of ≥8.5 mm measured by TVU (relative risk [RR], 6.418; 95% confidence interval [CI], 1.478-28.443) and an interval between CS and vaginal repair of ≤2.5 years (RR, 12.0; 95% CI, 1.541- 93.454) were good prognostic factors associated with surgery.Vaginal repair of CSD improved the symptoms of postmenstrual spotting and anatomically corrected the scars. An interval between C-section and a surgery of ≤2.5 years was optimal for vaginal repair, and a TRM at 6 months of ≥8.5 mm represented the standard healing of CSD.

Published
2016

26. Predictors of cesarean section in women with premature rupture of membranes

Author

Xipeng Wang, Qide Lin, Jianhua Lin, and Jieru Zhou

Subjects
medicine.medical_specialty, Fetal Membranes, Premature Rupture, Population, Prom, Risk model, Pregnancy, Risk Factors, medicine, Childbirth, Humans, education, Retrospective Studies, education.field_of_study, Obstetrics, business.industry, Cesarean Section, Obstetrics and Gynecology, Retrospective cohort study, General Medicine, medicine.disease, female genital diseases and pregnancy complications, Neonatal outcomes, Multivariate Analysis, Female, business, Premature rupture of membranes
Abstract

Whether occurring at preterm or term premature rupture of membranes (PROM) is associated with a series of adverse maternal and neonatal outcomes. Approximately 8% to 10% of women who experience PROM are delivered by cesarean section (CS). Under certain conditions it is appropriate to perform CS to reduce maternal and neonatal morbidity and mortality. This retrospective study was conducted at Renji Hospital affiliated to Shanghai Second Medical University to determine the significant predictors of CS in women whose pregnancies are complicated by PROM and construct a risk model of CS applicable to these women. (excerpt)

Published
2005

27. Human PBMC scRNA-seq-based aging clocks reveal ribosome to inflammation balance as a single-cell aging hallmark and super longevity.

Author

Hongming Zhu, Jiawei Chen, Kangping Liu, Lei Gao, Haiyan Wu, Liangliang Ma, Jieru Zhou, Zhongmin Liu, and Jing-Dong J. Han

Abstract

The article discuses quantification of aging rate using a blood single-cell RNA sequencing dataset for supercentenarians. It generates an aging clock model to determine the biological age of these individuals and finds that they exhibit specific changes in cell types and molecular hallmarks associated with slow aging and low inflammation. The study validates the role of ribosomal activity in maintaining this balance.

Published
2023
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