1. Secondary bile acids are associated with body lipid accumulation in obese pigs
- Author
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Yaolian Hu, Aimin Wu, Hui Yan, Junning Pu, Junqiu Luo, Ping Zheng, Yuheng Luo, Jie Yu, Jun He, Bing Yu, and Daiwen Chen
- Subjects
Lean or obese pig ,Lipid accumulation ,Bile acid profile ,Gut microbiota ,Animal culture ,SF1-1100 - Abstract
The aim of this study was to investigate the reasons for the differences in lipid accumulation between lean and obese pigs. The bile acids with varying levels within two types of pigs were found and then in vitro experiments were conducted to identify whether these bile acids can directly affect lipid accumulation. Fourteen pigs, including seven lean and seven obese pigs with body weights of approximately 80 kg, were fed the same diet at an amount approximately equivalent to 3% of their respective body weights daily for 42 d. In vitro, 3T3-L1 preadipocytes were cultured in medium with high glucose levels and were differentiated into mature adipocytes using differentiation medium. Then, bile acids were added to mature adipocytes for 4 d. The results showed that there was a difference in body lipids levels and gut microbiota composition between obese and lean pigs (P
- Published
- 2024
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