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Introduction: Numerous studies demonstrated that NLRP3 has been implicated in the pathogenesis of inflammatory bowel disease (IBD). Mesenchymal stem cells (MSCs) regulated the NLRP3 inflammasome, which has emerged as a novel therapeutic approach for treating IBD. Objectives: The exact role of NLRP3 in regulating MSCs’ function is unclear. Our study aimed to explore how NLRP3 affects the therapeutic effects of MSCs in colitis. Methods: We extracted MSCs from the bone marrow of C57BL/6 mice and Nlrp3 KO mice, and identified them using differentiation assays and flow cytometry. In vitro, Both WT MSCs and Nlrp3 KO MSCs were stimulated with inflammatory factor Lipopolysaccharide (LPS), and only WT MSCs were stimulated with varying concentrations of the NLRP3 inhibitor MCC950, then, quantified IL-10 levels in the supernatant. RNA-seq was performed to examine gene expression patterns and Seahorse was used to assess oxidative phosphorylation (OXPHOS) and glycolysis levels. Western blot was used to evaluate protein expression. In vivo, we treated DSS-induced colitis with either WT or Nlrp3 KO MSCs, monitoring weight, measuring colon length, and further evaluation. We also treated DSS-induced colitis with pretreated MSCs (BAY876, oe-Glut1, or oe-NLRP3), following the same experimental procedures as described above. Results: Our results demonstrate that Nlrp3 deletion did not affect MSC phenotypes, but rather promoted osteogenic differentiation. However, the absence of Nlrp3 reduced IL-10 production in MSCs in the presence of LPS, leading to impaired protection on DSS-induced colitis. Conversely, overexpression of NLRP3 promotes the production of IL-10, enhancing therapeutic effects. Further investigation revealed that Nlrp3 deficiency downregulated Glut1 expression and glycolysis activation in MSCs, resulting in decreased IL-10 production. Notably, overexpressing Glut1 in Nlrp3 KO MSCs restored their therapeutic effect that was previously dampened due to Nlrp3 deletion. Conclusion: Our findings demonstrate that NLRP3 heightens the therapeutic effects of MSC treatment on DSS-induced colitis.

Abstract The open‐circuit magnetic field of the V‐shaped interior PM (IPM) machine is predicted by the analytical method. The method is implemented following the basic principle of the subdomain method. The machine model is divided into five different regions, including rotor slot, magnetic barrier, air gap, stator slot, and stator slot opening. To perform the analytical prediction process in the polar coordinate system, these regions should be transformed into the appropriate subdomains. The last four regions can be transformed into the subdomains with the required shape based on the existing method. The rotor slot cannot be treated as a single subdomain, for the shape of which is irregular. Herein, the PM and vacuum region in the rotor slot is divided into several fan‐shaped subdomains. The division method is described clearly. Then, the boundary conditions alongside the interface between adjacent subdomains are derived based on function transformation. On this basis, the open‐circuit magnetic field of the IPM machine can be predicted. The magnetic field of the 6p/36 s V‐shaped IPM machine is obtained by the proposed method, finite element method simulation, and experiment, respectively. Additionally, the comparison results verify the accuracy and efficiency of the proposed method.

Abstract Aim This study aims to investigate the current status of work engagement among ophthalmic specialized nurses in China and explore the multifaceted factors influencing work engagement from the physical, psychological, and social perspectives. Methods This study adopts a cross-sectional survey design, distributed electronic questionnaires through WeChat software, and 261 valid questionnaires were received from ophthalmic specialized nurses. The survey includes demographic information about ophthalmic specialized nurses, as well as work engagement, sleep quality and social support. A generalized linear model was used to investigate the factors influencing the work engagement of ophthalmic specialized nurses. Results The overall work engagement score among ophthalmic specialized nurses in our study was 143.70 ± 13.66. Multivariate analysis showed that participation in teaching, professional title, sleep quality and perceived social support were the influencing factors of work engagement of ophthalmic specialized nurses. Discussion The results showed that ophthalmic specialized nurses with the title of “senior nurse”, participating in teaching had higher work engagement. According to the Two-Factor Theory, incentive factors can stimulate the work enthusiasm of specialized nurses. The worse the sleep quality of ophthalmic specialized nurses, the lower the work engagement. It is suggested that nursing managers should pay attention to the sleep problems of specialized nurses and reduce work burnout. The nurses who perceived higher levels of social support exhibited higher work engagement. Because social support can mitigate nurses’ work burnout and work-related stress. Conclusion Ophthalmic specialized nurses in China demonstrate a relatively high level of work engagement. Those with the title of senior nurse, involvement in teaching, better sleep quality, and higher perceived social support exhibit higher work engagement.

Abstract PA1895-1897 is a quorum sensing (QS) operon regulated by the anti-activator LuxR homologue QscR in Pseudomonas aeruginosa. We aimed to investigate its impact on bacterial metabolism, and whether it contributes to the delayed QS activation. We performed liquid chromatograph-mass spectrometer-based metabolomics using wildtype PAO1, PA1895-1897-knockout mutant, and mutant with pJN105.PA1895-1897 overexpression vector. The impact of metabolites on QS signaling molecule (3OC12-HSL and C4-HSL) concentrations, pyocyanin production, and QS gene (lasR, lasI, rhlR, and rhlI) expression was examined. Metabolomics analysis found that fatty acid biosynthesis had the highest fold enrichment among all metabolic pathways in PA1895-1897-overexpressed mutants. Among these enriched fatty acids, palmitoleic acid and acetic acid were the predominantly abundant ones that significantly affected by PA1895-1897 operon. When different doses of exogenous palmitoleic acid or acetic acid were added to the cultures of PA1895-1897 knockout mutants, their levels of 3OC12-HSL, C4-HSL, and pyocyanin were decreased in a dose-dependent manner. High doses of palmitoleic acid and acetic acid suppressed the mRNA expression of lasR, rhlR, and rhlI. Inhibition of fatty acid biosynthesis increased the production of 3OC12-HSL, C4-HSL, and pyocyanin in PA1895-1897-overexpressed cultures. Our data suggest that fatty acid synthesis is promoted by PA1895-1897 operon, and contributes the delayed expression of QS phenotypes, furthering the understanding about the regulation of bacterial QS activation.

Abstract Background Early dissemination to distant organs accounts for the dismal prognosis of patients with pancreatic ductal adenocarcinoma (PDAC). Chronic, dysregulated, persistent and unresolved inflammation provides a preferred tumor microenvironment (TME) for tumorigenesis, development, and metastasis. A better understanding of the key regulators that maintain inflammatory TME and the development of predictive biomarkers to identify patients who are most likely to benefit from specific inflammatory-targeted therapies is crucial for advancing personalized cancer treatment. Methods This study identified cell-specific expression of CALB2 in human PDAC through single-cell RNA sequencing analysis and assessed its clinicopathological correlations in tissue microarray using multi-color immunofluorescence. Co-culture systems containing cancer-associated fibroblasts (CAFs) and patient-derived organoids (PDOs) in vitro and in vivo were employed to elucidate the effects of CALB2-activated CAFs on PDAC malignancy. Furthermore, CUT&RUN assays, luciferase reporter assays, RNA sequencing, and gain- or loss-of-function assays were used to unravel the molecular mechanisms of CALB2-mediated inflammatory reprogramming and metastasis. Additionally, immunocompetent KPC organoid allograft models were constructed to evaluate CALB2-induced immunosuppression and PDAC metastasis, as well as the efficacy of inflammation-targeted therapy. Results CALB2 was highly expressed both in CAFs and cancer cells and correlated with an unfavorable prognosis and immunosuppressive TME in PDAC patients. CALB2 collaborated with hypoxia to activate an inflammatory fibroblast phenotype, which promoted PDAC cell migration and PDO growth in vitro and in vivo. In turn, CALB2-activated CAFs upregulated CALB2 expression in cancer cells through IL6-STAT3 signaling-mediated direct transcription. In cancer cells, CALB2 further activated Ca2+-CXCL14 inflammatory axis to facilitate PDAC metastatic outgrowth and immunosuppression. Genetic or pharmaceutical inhibition of CXCL14 significantly suppressed CALB2-mediated metastatic colonization of PDAC cells in vivo and extended mouse survival. Conclusions These findings identify CALB2 as a key regulator of inflammatory reprogramming to promote PDAC metastatic progression. Combination therapy with αCXCL14 monoclonal antibody and gemcitabine emerges as a promising strategy to suppress distant metastasis and improve survival outcomes in PDAC with CALB2 overexpression.

Abstract We investigated the clinical characteristics of primary aldosteronism (PA) screened from patients with hypertension in China. The participants were hypertensive patients who were suspected of PA and registered in the China Primary Aldosteronism Prospective Study. Plasma aldosterone‐to‐renin ratio (ARR) was used as the screening test. In patients screened positive for PA, that is, an ARR exceeding the thresholds and plasma aldosterone concentration (PAC) > 100 pg/mL, a confirmatory test was performed for diagnosis. Patients with PA underwent a CT scan and adrenal venous sampling for subtyping. Of the 1497 screened patients, 754 (50.4%) had an ARR exceeding the diagnostic threshold and 637 (84.5% of those eligible) were registered. These registered hypertensive patients with suspected PA had a mean (standard deviation) age of 52.6 ± 12.1 years, and included 442 (58.6%) women. In multiple stepwise logistic regression, the significant odds ratios for the presence of diagnosed (n = 490) versus suspected and non‐diagnosed PA (n = 147) were 4.54 (95% CI: 2.78‐7.39) for a history of hypokalemia, 0.79 (95% CI: 0.64‐0.98) for a 0.9 mmol/l higher serum total cholesterol, and 2.25 (95% CI: 1.63‐3.10) for a doubling of PAC in the supine or standing/sitting position. In multiple stepwise logistic regression, the significant odds ratios for the presence of unilateral (n = 135) versus bilateral PA (n = 53) were 3.04 (95% CI: 1.90‐4.87) for a 0.4 mmol/l lower minimum serum potassium concentration and 1.86 (95% CI: 1.20‐2.86) for a 0.3 mmol/l higher serum high‐density lipoprotein cholesterol. PA might be a biochemical continuum in the adrenal hypersecretion of aldosterone as well as hypokalemia.

Abstract Objective To investigate the current situation of innovative behavior and organizational structure authorization of ophthalmic specialty nurses in China, and analyze the influencing factors of innovative behavior. Methods A cross-sectional survey was carried out among 301 ophthalmic specialty nurses in China, representing Representing 82 hospitals in 25 provinces, using a general data questionnaire, Innovative Behavior Scale(IBS), and conditions of work effectiveness questionnaire(CWEQ-II). Results The mean total score for innovative behavior among Chinese ophthalmic specialty nurses was (4.73 ± 1.14), and the mean total score for organizational structure authorization was (61.65 ± 14.69). The logistic regression analysis revealed that organizational structure authorization, hospital grade, and ophthalmic specialty nurses primarily engaged in clinical care and clinical research had statistically significant impacts on the level of innovative behavior (P

Purpose This study compared the efficacy of heat insulation between 5% dextrose and 0.9% saline in radiofrequency ablation (RFA). Accordingly, temperature variations and maximum temperatures were assessed at identical distances and heat field distributions. Methods Cubes of porcine liver tissue, measuring 10 mm across, were selected to precisely align the ablation boundary with the tissue boundary. An 18-gauge electrode with a 7-mm tip was inserted into each cube (10 per group) in a stainless-steel cup containing 40 mL of 5% dextrose or 0.9% saline. Fixed ablation was performed for 3 minutes using continuous mode at 30 W, simulating the typical thermal environment during thyroid RFA. Real-time temperature measurements were recorded by sensors positioned 0, 1, 3, and 5 mm from the cube’s edge. A comparative analysis was conducted to assess the maximum temperature, temperature variation, and duration of temperatures exceeding 42℃. Results In both groups, the temperature curve declined with increasing distance from the edge of the ablated tissue. However, 0.9% saline exhibited higher maximum temperatures at 1, 3, and 5 mm compared to 5% dextrose (1 mm: 44.55°C±5.25°C vs. 34.68°C±3.07°C; 3 mm: 39.64°C±2.53°C vs. 29.22°C±2.21°C; 5 mm: 38.86°C±2.14°C vs. 28.74°C±2.51°C; all P

AIM: To investigate the current situation and influencing factors of self-management ability in dry eye patients in west China. METHODS: A total of 265 patients clinically diagnosed with dry eye received a convenience survey questionnaire at West China Hospital of Sichuan University. All participants completed the rating scale of health self-management skill for adults (AHSMSRS), Huaxi Emotional-Distress Index (HEI), e-health literacy scale (e-HEALS) and Brief Illness Perception Questionnaire (Brief-IPQ). A generalized linear model was employed to establish a multivariate linear model with demographic data, psychological state, e-HEALS, and illness perception as independent variables and health self-management skill score as the dependent variable. RESULTS: The mean score for health self-management skill was 165.58±15.79. Multivariate analysis revealed that advanced age, better illness perception and improved psychological state were associated with better health self-management ability among dry eye patients. Furthermore, the health self-management ability of patients with a disease duration less than 1y was found to be higher compared to those with a disease duration exceeding 1y. CONCLUSION: The health self-management ability of dry eye patients in west China is relatively high. Age, duration of disease, illness perception and psychological state are the influencing factors on the health self-management ability of dry eye patients.

Abstract Background To provide a novel classification for all implants in the maxillary retromolar region to simplify surgical design, reduce surgical risks, and guide clinicians in clinical decision-making. Methods A total of 180 patients with bilateral partial or completely edentulous atrophic posterior maxillae who had received or were scheduled to receive pterygomaxillary implants were included in this study. Cone-beam computed tomography was performed, and the sagittal and coronal images were acquired at 110 kV and 10 mA. The exposure volume was 120 mm in diameter and 80 mm in height. The pterygomaxillary implants were divided into three different types based on the anatomical structures the implants passed through. Results The average age of the 180 patients was 69 (range: 39–89) years; 99 were men and 81 were women. All the patients exhibited 360 pterygomaxillary implant sites. However, during mimic implantation, 14 implant sites were excluded due to severe resorption of the tuberosity, very small pterygoid plates, or variations in the descending palatal artery configuration. Of the 346 pterygomaxillary implant sites, 24.0% (83/346), 40.7% (141/346), 22.0% (76/346), and 13.3% (46/346) were classified as Types I, IIa, IIb, and III, respectively. Conclusions Type II pterygomaxillary implants were the most commonly used in the novel classification. Different types of pterygomaxillary implants should follow specific designs and surgical strategies to achieve optimal outcomes.

This is an article in the field of metallurgical engineering, which proposed a new idea of mixing molten blast furnace slag with steel slag to achieve self-pulverization of steel slag, and systematically studied the influence mechanism of special components of CaF2 and CeO2 in blast furnace slag of Baotou Iron and Steel Company on the self-pulverization of steel slags. Using analytically pure CaO, SiO2, CaF2 and CeO2 as starting materials, pure C2S specimens were prepared with n ( CaO ) : n ( SiO2 ) = 2 : 1, then different mass fractions of CaF2 and CeO2 were added, respectively. The effect of CaF2 and CeO2 on the self-pulverization behavior of C2S was investigated by calcining the specimen at 1 450 °C for 1 h and cooling with the furnace. The results showed when CaF2 was incorporated into C2S and the content of CaF2 was less than 2.5%, it had little effect on the self-pulverization of C2S. However, when the content of CaF2 was more than 2.5%, the specimen had different degrees of agglomeration, which affected the self-pulverization of C2S. Considering that excessive CaF2 would affect the environment, the content of CaF2 should be controlled below 2.5%. When CeO2 incorporated into C2S and the content was 0.25%, C2S could still be completely self-pulverized, but when the CeO2 content was more than 0.5%, C2S no longer self-pulverized.Therefore, the CeO2 content must be less than 0.25 %, and the self-pulverization effect of C2S was relatively good.

Abstract Amino acid metabolism plays a pivotal role in tumor microenvironment, influencing various aspects of cancer progression. The metabolic reprogramming of amino acids in tumor cells is intricately linked to protein synthesis, nucleotide synthesis, modulation of signaling pathways, regulation of tumor cell metabolism, maintenance of oxidative stress homeostasis, and epigenetic modifications. Furthermore, the dysregulation of amino acid metabolism also impacts tumor microenvironment and tumor immunity. Amino acids can act as signaling molecules that modulate immune cell function and immune tolerance within the tumor microenvironment, reshaping the anti-tumor immune response and promoting immune evasion by cancer cells. Moreover, amino acid metabolism can influence the behavior of stromal cells, such as cancer-associated fibroblasts, regulate ECM remodeling and promote angiogenesis, thereby facilitating tumor growth and metastasis. Understanding the intricate interplay between amino acid metabolism and the tumor microenvironment is of crucial significance. Expanding our knowledge of the multifaceted roles of amino acid metabolism in tumor microenvironment holds significant promise for the development of more effective cancer therapies aimed at disrupting the metabolic dependencies of cancer cells and modulating the tumor microenvironment to enhance anti-tumor immune responses and inhibit tumor progression.

Rapid heating is a promising technique for achieving excellent mechanical properties. This study innovatively applies rapid heating to a novel Ti–Mo martensite steel through in-situ observation experiments. By comparing the microstructure evolutions at heating rates of 1 and 30 °C/s, the relationship between microstructure and property evolution was elucidated. The results indicate that the refined austenite grains resulting from rapid heating stem from enhanced grain nucleation, accumulated dislocations, and the increased formation of new carbides under the large superheat. Furthermore, carbide maturation predominates during the slow heating process, while rapid heating accelerates new carbide precipitation with limited carbide maturation. Moreover, during rapid heating, both coherent and non-coherent relationships were observed between the (Ti, Mo, Fe)C particles and the martensite matrix, demonstrating a remarkable pinning effect on grain boundary migration. Nevertheless, during slow heating, large-sized (Ti, Mo)C carbides were formed, presenting a non-coherent relationship with the martensite matrix. Additionally, the primary differences in strength caused by rapid heating compared to slow heating include grain refinement, dislocation, and precipitation strengthenings, with dislocation strengthening playing the dominant role.

To address the issues of inadequate topsoil structure, soil erosion, and low soil fertility in the reclamation of alpine mining areas. Taking Qinghai West Copper Mine as an example, this study investigates the impact of three materials—sheep manure(A), TG modifier(B), and water retaining agent(C)—on soil improvement using a simulated orthogonal design experiment. The experiment aims to identify the optimal mixing ratio that yields the most significant improvement. Orthogonal polar results revealed that sheep manure had a more pronounced effect on soil organic matter, soil bulk density, porosity, and total salt content. Meanwhile, TG modifier demonstrated a stronger impact on pH, and the water retaining agent exhibited a greater influence on water-holding capacity in the field. The combined results of extreme difference analysis and principal component analysis indicated that treatment 1(A 6 g/kg, B 1.85 g/kg, C 0.06 g/kg) represented the optimal combination of physicochemical properties for soil improvement. Redundancy analyses yielded that sheep manure and TG modifier explained the best variability in soil physicochemical properties. Organic matter, soil field water holding capacity, and porosity exhibited significant correlations with environmental materials. Organic matter displayed a positive correlation with sheep manure, while porosity and field water holding capacity were positively associated with water retaining agent and TG modifier. Conversely, pH, total salt content, and soil bulk density demonstrated weaker correlations with environmental materials. Porosity and field water holding capacity were strongly positively correlated and both were negatively correlated with organic matter. Considering the optimal combination of soil organic matter (A3B3C2) and other soil physicochemical properties (A1B1C1), the optimal formula for soil matrix restoration in alpine mining areas was determined using the median algorithm as A2B2C1.5. This corresponds to 12 g/kg of A (sheep manure), 3.7 g/kg of B (TG modifier), and 0.09 g/kg of C (water retaining agent) as the optimal ratios. The engineering application process, primarily centered around activities such as slope trimming, hanging coconut/barbed wire, hanging ecological rods, setting up drainage ditches, screening of guest soil, spraying of alien soil, laying of water-preserving blankets, and maintenance management and monitoring, was summarized. This summary integrates research on experimental formulations with practical engineering applications. The results of the engineering applications of the three environmental materials illustrate that soil physicochemical properties and biomass were also significantly improved. This study provides a reference to address the restoration of soil fertility and structure in high-altitude mining areas of Qinghai-Tibet, which is of great significance for soil and water conservation.

Abstract The deep connection among braids, knots and topological physics has provided valuable insights into studying topological states in various physical systems. However, identifying distinct braid groups and knot topology embedded in non-Hermitian systems is challenging and requires significant efforts. Here, we demonstrate that an unsupervised learning with the representation basis of s u(n) Lie algebra on n-fold extended non-Hermitian bands can fully classify braid group and knot topology therein, without requiring any prior mathematical knowledge or any pre-defined topological invariants. We demonstrate that the approach successfully identifies different topological elements, such as unlink, unknot, Hopf link, Solomon ring, trefoil, and so on, by employing generalized Gell-Mann matrices in non-Hermitian models with n=2 and n=3 energy bands. Moreover, since eigenstate information of non-Hermitian bands is incorporated in addition to eigenvalues, the approach distinguishes the different parity-time symmetry and breaking phases, recognizes the opposite chirality of braids and knots, and identifies out distinct topological phases that were overlooked before. Our study shows significant potential of machine learning in classification of knots, braid groups, and non-Hermitian topological phases.

Abstract Hemangioma of infancy is the most common vascular tumor during infancy and childhood. Despite the proven efficacy of propranolol treatment, certain patients still encounter resistance or face recurrence. The need for frequent daily medication also poses challenges to patient adherence. Bleomycin (BLM) has demonstrated effectiveness against vascular anomalies, yet its use is limited by dose-related complications. Addressing this, this study proposes a novel approach for treating hemangiomas using BLM-loaded hyaluronic acid (HA)-based microneedle (MN) patches. BLM is encapsulated during the synthesis of polylactic acid (PLA) microspheres (MPs). The successful preparation of PLA MPs and MN patches is confirmed through scanning electron microscopy (SEM) images. The HA microneedles dissolve rapidly upon skin insertion, releasing BLM@PLA MPs. These MPs gradually degrade within 28 days, providing a sustained release of BLM. Comprehensive safety assessments, including cell viability, hemolysis ratio, and intradermal reactions in rabbits, validate the safety of MN patches. The BLM@PLA-MNs exhibit an effective inhibitory efficiency against hemangioma formation in a murine hemangioma model. Of significant importance, RNA-seq analysis reveals that BLM@PLA-MNs exert their inhibitory effect on hemangiomas by regulating the P53 pathway. In summary, BLM@PLA-MNs emerge as a promising clinical candidate for the effective treatment of hemangiomas. Graphical Abstract

Abstract Systemic lupus erythematosus (SLE), a chronic autoimmune disease, is marked by impaired immune tolerance and heightened activity in both innate and adaptive immune systems. Hallmarks of SLE include elevated type I interferons and autoantibody production, though the exact causes of SLE remain elusive despite advances in research techniques. Crucial to SLE research is understanding its pathogenesis and developing effective diagnostic and therapeutic methods. Recent studies have highlighted a significant link between mitochondrial abnormalities and SLE's development and progression. Mitochondrial dysfunction plays a key role in SLE pathogenesis through various mechanisms such as mitochondrial DNA mutations, oxidative stress, immune metabolic reprogramming, and cellular death. These factors collectively contribute to the loss of self‐tolerance, increased autoantibody production, and impaired clearance of immune complexes, leading to their deposition. This triggers sustained inflammatory responses and damages multiple organs. This review focuses on the diagnostic and therapeutic approaches related to mitochondrial abnormalities in SLE. Targeting mitochondrial pathways presents a promising strategy for treating SLE, potentially improving prognosis and quality of life for those affected. Future research should further investigate the role of mitochondria in the onset and progression of SLE, providing new avenues for understanding and managing this complex disease.

Abstract The far-from-equilibrium solidification during additive manufacturing often creates large residual stresses that induce solid-state cracking. Here we present a strategy to suppress solid-state cracking in an additively manufactured AlCrFe2Ni2 high-entropy alloy via engineering phase transformation pathway. We investigate the solidification microstructures formed during laser powder-bed fusion and directed energy deposition, encompassing a broad range of cooling rates. At high cooling rates (104−106 K/s), we observe a single-phase BCC/B2 microstructure that is susceptible to solid-state cracking. At low cooling rates (102−104 K/s), FCC phase precipitates out from the BCC/B2 matrix, resulting in enhanced ductility (~10 %) and resistance to solid-state cracking. Site-specific residual stress/strain analysis reveals that the ductile FCC phase can largely accommodate residual stresses, a feature which helps relieve residual strains within the BCC/B2 phase to prevent cracking. Our work underscores the value of exploiting the toolbox of phase transformation pathway engineering for material design during additive manufacturing.

Abstract We study holographic supersymmetric Rényi entropies from a family of hyperbolic black holes in an Einstein-Maxwell-dilaton (EMD) system under the BPS condition. We calculate the thermodynamic quantities of these hyperbolic black holes. We find a remarkably simple formula of the supersymmetric Rényi entropy that unifies (interpolates) 11 cases embeddable to 10 or 11 dimensional supergravity. It reproduces many known results in the literature, and gives new results with distinctive features. We show that the supersymmetric version of the modular entropy and the capacity of entanglement cannot be mapped to thermal quantities, due to the dependence of the temperature and the chemical potential by the BPS condition. We also calculate the entanglement spectrum. We derive the potential of the EMD system from a V = 0 solution and obtain two neutral solutions with scalar hair as a byproduct.

Background Bendamustine–rituximab (BR) therapy stands out as a promising alternative for elderly patients with diffuse large B-cell lymphoma (DLBCL), demonstrating notable efficacy when conventional regimens pose challenges. Despite its clinical success, the intricate mechanisms underlying BR therapy have remained elusive.Methods DLBCL cell lines were used to investigate the mechanism of BR therapy in vitro. RNA-seq and Western blot were used to explore the target pathways of BR therapy. STING was knocked out using Crispr-cas9 and inhibited using H-151 to investigate its role in BR therapy. Bulk RNA-seq and single-cell RNA-seq data from patients were analyzed to investigate the association between STING and pyroptosis pathways, validated using STING downregulated cells. Flow cytometry, transwell experiments and co-culture experiments were performed to investigate the inflammatory phenotype of DLBCL cells after BR treatment and its effect on T-cell recruitment and activation.Results This study elucidates that BR elicits direct tumoricidal effects by promoting apoptosis and inducing cell cycle arrest. The synergistic impact with rituximab is further potentiated by complement addition, demonstrating the pivotal role of in vivo antibody-dependent cellular cytotoxicity. Moreover, our investigation reveals that, through a cGAS–STING-dependent pathway, prolonged exposure to BR induces pyroptosis in DLBCL cells. Activation of the cGAS–STING pathway by BR therapy triggers the release of inflammatory factors and upregulates major histocompatibility complex molecules, shaping an immunologically hot tumor microenvironment.Conclusions This unique dual influence not only directly targets DLBCL cells but also engages the patient’s immune system, paving the way for innovative combination therapies. The study provides comprehensive insights into the multifaceted actions of BR in DLBCL, offering a foundation for refined and personalized treatment strategies in elderly patients.

In order to accurately measure the level of competency frustration and what measures to take to alleviate the negative effects of competency frustration, 35 graduate students were selected to verify the effect of the frontal lobe α asymmetry (FAA) as a judgement of the competence frustration level using EEG experimental method. On this basis, through two stopwatch stopping experiments, 108 fire rescue personnel were selected to conduct the experiments to investigate the intervention effects of developmental feedback and compassion-focused therapy in turn. The results showed that frontal α asymmetry could be used as an EEG indicator for judging competency frustration, and the intervention method of compassion-focused therapy reduced the level of competency frustration of the subjects, while developmental feedback interventions did the opposite. The difference in the effects of the two intervention methods indicates that when intervening in competence frustration, it is easier to reduce the competency frustration by focusing on the subjects themselves than focusing on the completion of the task.

ABSTRACT Pancreatic cancer, predominantly pancreatic ductal adenocarcinoma (PDAC), is one of the most malignant tumors of the digestive system. Emerging evidence suggests the involvement of the microbiome and metabolic substances in the development of PDAC, yet the results remain contradictory. This study aims to identify the alterations and relationships in intratumoral microbiome and metabolites in PDAC. We collected matched tumor and normal adjacent tissue (NAT) samples from 105 PDAC patients and performed a 6-year follow-up. 2bRAD-M sequencing, untargeted liquid chromatography-tandem mass spectrometry, and untargeted gas chromatography-mass spectrometry were performed. Compared with NATs, microbial α-diversity decreased in PDAC tumors. The relative abundance of Staphylococcus aureus, Cutibacterium acnes, and Cutibacterium granulosum was higher in PDAC tumor after adjusting for confounding factors body mass index and M stage, and the presence of Ralstonia pickettii_B was found associated with a worse overall survival. Metabolomic analysis revealed distinctive differences in composition between PDAC and NAT, with 553 discriminative metabolites identified. Differential metabolites were revealed to originate from the microbiota and showed significant interactions with shifted bacterial species through KO (KEGG Orthology) genes. These findings suggest that the PDAC microenvironment harbors unique microbial-derived enzymatic reactions, potentially influencing the occurrence and development of PDAC by modulating the levels of glycerol-3-phosphate, succinate, carbonate, and beta-alanine.IMPORTANCEWe conducted a large sample-size pancreatic adenocarcinoma microbiome study using a novel microbiome sequencing method and two metabolomic assays. Two significant outcomes of our analysis are: (i) commensal opportunistic pathogens Staphylococcus aureus, Cutibacterium acnes, and Cutibacterium granulosum were enriched in pancreatic ductal adenocarcinoma (PDAC) tumors compared with normal adjacent tissues, and (ii) worse overall survival was found related to the presence of Ralstonia pickettii_B. Microbial species affect the tumorigenesis, metastasis, and prognosis of PDAC via unique microbe-enzyme-metabolite interaction. Thus, our study highlights the need for further investigation of the potential associations between pancreatic microbiota-derived omics signatures, which may drive the clinical transformation of microbiome-derived strategies toward therapy-targeted bacteria.

Pancreatic cancer, a highly fatal malignancy, is predicted to rank as the second leading cause of cancer-related death in the next decade. This highlights the urgent need for new insights into personalized diagnosis and treatment. Although molecular subtypes of pancreatic cancer were well established in genomics and transcriptomics, few known molecular classifications are translated to guide clinical strategies and require a paradigm shift. Notably, chronically developing and continuously improving high-throughput technologies and systems serve as an important driving force to further portray the molecular landscape of pancreatic cancer in terms of epigenomics, proteomics, metabonomics, and metagenomics. Therefore, a more comprehensive understanding of molecular classifications at multiple levels using an integrated multi-omics approach holds great promise to exploit more potential therapeutic options. In this review, we recapitulated the molecular spectrum from different omics levels, discussed various subtypes on multi-omics means to move one step forward towards bench-to-beside translation of pancreatic cancer with clinical impact, and proposed some methodological and scientific challenges in store.

ObjectiveThis study investigates peer effects on the health behaviors of middle-aged and older adult people in China and examines the moderating role of Internet use.MethodsA total of 16,188 respondents were selected from the China Health and Retirement Longitudinal Study (CHARLS) 2020 data set. Ordinary Least Squares and Quantile Regression were used to validate the peer effect of health behaviors on middle-aged and older adults, and a moderating effects model was used to test the moderating roles of Internet use and Internet proficiency. Finally, the peer effect was analyzed for heterogeneity according to Internet use preferences.ResultsThe peer effect had a positive influence on the individual health behaviors of middle-aged and older adult people in China (β = 0.5341, p

Pancreatic cancer is an extremely malignant tumor. PVT1 and MYC signaling has been considered as a therapeutic target recently. Nonetheless, the prognostic values and critical regulatory networks of PVT1-MYC duet in pancreatic cancer remain unclear. Firstly, we identified PVT1-MYC duet-related genes using public databases. Then we analyzed our Hi-C and ChIP-seq data to confirm PVT1-MYC duet. We performed LASSO regression and multivariate Cox regression analysis to build a prognostic model whose effectiveness and robustness were validated by Cox regression, ROC analysis, calibration curve, and nomogram. Besides, we conducted functional enrichment analyses, mutation profiles analyses and the immune features analyses to compare low- and high-risk group. Functional enrichment analyses revealed that several terms associated with cancer progression were enriched in the high-risk group. Mutation profile analysis showed that high-risk group had higher tumor mutation burden, and immune analysis demonstrated high-risk group had more immunosuppressive tumor microenvironment. Finally, we detected PVT1 expression in pancreatic cancer and paracancer tissues from the PUMCH cohort, which showed that PVT1 was significantly upregulated in pancreatic cancer and associated with invasion, metastasis, and poor prognosis. We further performed transwell and proliferation assays and found that PVT1, CDC6, and COL17A1 could promote migration or proliferation of PDAC cells. This study constructed a prognostic model based on three PVT1-MYC duet-related genes, which had a significant potential in predicting the prognosis and tumor microenvironment of pancreatic cancer. These results suggested that targeting PVT1-MYC duet or its regulatory processes could be a therapeutic option with great interests.

Abstract Topological phononic cavities, such as ring resonators with topological whispering gallery modes (TWGMs), offer a flexible platform for the realization of robust phononic circuits. However, the chiral mechanism governing TWGMs and their selective routing in integrated phononic circuits remain unclear. This work reveals, both experimentally and theoretically, that at a phononic topological interface, the elastic spin texture is intricately linked to, and can be explained through a knowledge of, the phonon eigenmodes inside each unit cell. Furthermore, for paired, counterpropagating TWGMs based on such interfaces in a waveguide resonator, this study demonstrates that the elastic spin exhibits locking at discrete frequencies. Backed up by theory, experiments on kHz TWGMs in thin honeycomb‐lattice aluminum plates bored with clover‐leaf shaped holes show that together with this spin‐texture related angular‐momentum locking mechanism at a single topological interface, there are triplicate parity‐frequency‐space selective wave routing mechanisms. In the future, these mechanisms can be harnessed for the versatile manipulation of elastic‐spin based routing in phononic topological insulators.

The rapid heating treatment with different tempering temperatures of 160, 200 and 320 °C was innovatively applied in one developed martensitic wear-resistant steel. The elaborate characterization on the evolution of microstructure-mechanical properties including the wear performance in different tempering temperatures were compared. Results manifested that the rapid heating treatment yielded a peak performance in the martensitic wear-resistant steel when tempered at 200 °C, by which the tensile strength and elongation were 1762.46 MPa and 13.13%, respectively, accompanied by the highest product of strength and elongation of 23.14 GPa·%. The enhanced work hardening contribution caused by the refined martensitic laths and carbides, as well as the appropriate recovery leaded to the peak performance at 200 °C. However, the mechanical properties both deteriorated by tempering at 160 and 320 °C subjecting to either the stress concentration or the coarsen carbides. Besides, the Baker–Nutting relationship between carbides and the matrix was established and furtherly proved the coarsen carbides resulted in the worst elongation by tempering at 320 °C. In addition, the main strength differences caused by different tempering temperatures were the grain refinement strengthening, dislocation strengthening, and precipitation strengthening, in which the grain refinement and precipitation strengthening played the dominating role. The current findings provide foundation for the industrial application of rapid heating techniques on the high-performance martensitic wear-resistant steels.

We used powder hot extrusion followed by annealing to fabricate a dual-phase AlCoCrFeNi2.1 eutectic high-entropy alloy consisting of both non-recrystallized and recrystallized grains with dense nano-sized precipitates arranged in heterogeneous structures. The resulting alloy exhibits superior tensile properties with a yield strength of ∼1.2 GPa, an ultimate tensile strength of ∼1.5 GPa, and a uniform elongation of ∼18%. Such multiscale microstructure results in exceptional strain hardening of the AlCoCrFeNi2.1 alloy by activating remarkable hetero-deformation induced (HDI) stresses. Our study provides an effective approach to fabricating high-performance structural materials via dual-phase heterogeneous microstructure design.

Abstract Background Pancreatic ductal adenocarcinoma (PDAC) is a lethal malignancy with high probability of recurrence and distant metastasis. Liver metastasis is the predominant metastatic mode developed in most pancreatic cancer cases, which seriously affects the overall survival rate of patients. Abnormally activated endoplasmic reticulum stress and lipid metabolism reprogramming are closely related to tumor growth and metastasis. This study aims to construct a prognostic model based on endoplasmic reticulum stress and lipid metabolism for pancreatic cancer, and further explore its correlation with tumor immunity and the possibility of immunotherapy. Methods Transcriptomic and clinical data are acquired from TCGA, ICGC, and GEO databases. Potential prognostic genes were screened by consistent clustering and WGCNA methods, and the whole cohort was randomly divided into training and testing groups. The prognostic model was constructed by machine learning method in the training cohort and verified in the test, TCGA and ICGC cohorts. The clinical application of this model and its relationship with tumor immunity were analyzed, and the relationship between endoplasmic reticulum stress and intercellular communication was further explored. Results A total of 92 characteristic genes related to endoplasmic reticulum stress, lipid metabolism and liver metastasis were identified in pancreatic cancer. We established and validated a prognostic model for pancreatic cancer with 7 signatures, including ADH1C, APOE, RAP1GAP, NPC1L1, P4HB, SOD2, and TNFSF10. This model is considered to be an independent prognosticator and is a more accurate predictor of overall survival than age, gender, and stage. TIDE score was increased in high-risk group, while the infiltration levels of CD8+ T cells and M1 macrophages were decreased. The number and intensity of intercellular communication were increased in the high ER stress group. Conclusions We constructed and validated a novel prognostic model for pancreatic cancer, which can also be used as an instrumental variable to predict the prognosis and immune microenvironment. In addition, this study revealed the effect of ER stress on cell–cell communication in the tumor microenvironment.

Abstract Background Paired box 1 (PAX1) is a transcription factor and essential for the development of pharyngeal pouches-derived tissues, including thymus. PAX1 mutations are identified in Severe Combined Immunodeficiency (SCID) patients with Otofaciocervical Syndrome Type 2 (OTFCS2). However, despite the critical roles of PAX1 in embryonic development and diseases, detailed insights into its molecular mode of action are critically missing. Methods The repressing roles of PAX1 and SCID associated mutants on Wnt signaling pathway were investigated by luciferase reporter assays, qRT-PCR and in situ hybridization in HEK293FT, HCT116 cells and zebrafish embryos, respectively. Co-immunoprecipitation (co-IP) and western blotting assays were carried out to identify the molecular mechanisms underlying PAX1’s role on Wnt signaling pathway. hESC based endoderm differentiation, flow cytometry, high-throughput sequencing data analysis, and qRT-PCR assays were utilized to determine the roles of PAX1 during endoderm differentiation. Results Here, we show that PAX1 represses canonical Wnt signaling pathway in vertebrate cells. Mechanically, PAX1 competes with SUMO E3 ligase PIASy to bind to TCF7L2, thus perturbing TCF7L2 SUMOylation level, further reducing its transcriptional activity and protein stability. Moreover, we reveal that PAX1 plays dual roles in hESC-derived definitive and foregut/pharyngeal endoderm cells, which give rise to the thymus epithelium, by inhibiting Wnt signaling. Importantly, our data show PAX1 mutations found in SCID patients significantly compromise the suppressing ability of PAX1 on Wnt signaling. Conclusions Our study presents a novel molecular mode of action of PAX1 in regulation of canonical Wnt signaling and endoderm differentiation, thus providing insights for the molecular basis of PAX1 associated SCID, offering better understanding of the behavior of PAX1 in embryogenesis.

Abstract The pumped storage power station (PSPS) is an important measure to achieve the strategic goal of “dual carbon”. As one of the preferred types for the upper reservoir dams of PSPSs, the concrete-faced rockfill dam (CFRD) often has a dam foundation on a steep transverse slop and is prone to produce slip deformation along the slope, resulting in poor anti-sliding stability of the dam slope. It is dangerous for the operation safety of PSPSs. Therefore, the slip deformation of CFRDs on dam foundations with large dip angles is investigated. The mechanism for the initiation of slip deformation is revealed. The design measures of physical mechanic and geometric structure are proposed to reduce slip deformation. The results show that the larger sliding forces and smaller anti-sliding forces are the fundamental reasons that CFRDs on dam foundations with large dip angles are prone to produce slip deformation. The larger the dip angle of the dam foundation, the larger the slip deformation of the dam body and face slab, and the smaller the safety factor of the dam slope. When the dip angle of the dam foundation is greater than 15°, the safety factor of the dam slope is less than the minimum value of 1.5 required by codes. The addition of pressure slopes can effectively reduce the slip deformation of the dam body or face slab and significantly improve the anti-sliding stability of the dam slope. When the height or width of the pressure slope platform is greater and the cohesion or internal friction angle of the pressure slope is larger, the slip deformations of the dam body and face slab are smaller, and the safety factor of the dam slope is greater. It is recommended that the height and width of the pressure slope platform be 1/2 times the maximum height of the main dam, and the density (cohesion and internal friction angle) of the pressure slope be equivalent to that of the main dam’s rockfill material. The research results can provide theoretical and technical support for the design and construction of CFRDs for the upper reservoir of PSPSs.

Few-shot fine-grained image classification (FSFGIC) methods refer to the classification of images (e.g., birds, flowers, and airplanes) belonging to different subclasses of the same species by a small number of labeled samples. Through feature representation learning, FSFGIC methods can make better use of limited sample information, learn more discriminative feature representations, greatly improve the classification accuracy and generalization ability, and thus achieve better results in FSFGIC tasks. In this paper, starting from the definition of FSFGIC, a taxonomy of feature representation learning for FSFGIC is proposed. According to this taxonomy, we discuss key issues on FSFGIC (including data augmentation, local and/or global deep feature representation learning, class representation learning, and task-specific feature representation learning). In addition, the existing popular datasets, current challenges and future development trends of feature representation learning on FSFGIC are also described.

Abstract Hyperuricemia (HUA) is a metabolic syndrome caused by abnormal purine metabolism. Although recent studies have noted a relationship between the gut microbiota and gout, whether the microbiota could ameliorate HUA-associated systemic purine metabolism remains unclear. In this study, we constructed a novel model of HUA in geese and investigated the mechanism by which Lactobacillus rhamnosus GG (LGG) could have beneficial effects on HUA. The administration of antibiotics and fecal microbiota transplantation (FMT) experiments were used in this HUA goose model. The effects of LGG and its metabolites on HUA were evaluated in vivo and in vitro. Heterogeneous expression and gene knockout of LGG revealed the mechanism of LGG. Multi-omics analysis revealed that the Lactobacillus genus is associated with changes in purine metabolism in HUA. This study showed that LGG and its metabolites could alleviate HUA through the gut-liver-kidney axis. Whole-genome analysis, heterogeneous expression, and gene knockout of LGG enzymes ABC-type multidrug transport system (ABCT), inosine-uridine nucleoside N-ribohydrolase (iunH), and xanthine permease (pbuX) demonstrated the function of nucleoside degradation in LGG. Multi-omics and a correlation analysis in HUA patients and this goose model revealed that a serum proline deficiency, as well as changes in Collinsella and Lactobacillus, may be associated with the occurrence of HUA. Our findings demonstrated the potential of a goose model of diet-induced HUA, and LGG and proline could be promising therapies for HUA.

The two-spotted spider mite, Tetranychus urticae Koch, is one of the most harmful pests in many agroecosystems worldwide. To effectively manage this pest, there is an urgent need to develop novel bio-active acaricides that support integrated pest management strategies targeting T. urticae. In this study, we explored the acaricidal effects of xenocoumacin 1 (Xcn1) on T. urticae and its predator Neoseiulus californicus using the highly purified compound. Xcn1 was extracted and purified from the cell-free supernatant of the Xenorhabdus nematophila CB6 mutant constructed by the easy promoter activated compound identification (easyPACId) method. When the concentration of Xcn1 exceeded 100 μg mL−1, the survival rate of spider mite adults declined to below 40% and the fecundity was decreased by 80% at six days post-application. At concentrations of 25 and 50 μg mL−1, Xcn1 significantly impeded spider mite development by inhibiting the molt. However, neither concentration had any adverse effects on the survival or reproduction of the predatory mite N. californicus. The results from laboratory and semi-field experiments consistently demonstrated the effectiveness of the antimicrobial metabolite Xcn1 in controlling pest mites at both the molecular and physiological levels. Our study offers a promising possibility that combines the compatible biocontrol agents of Xcn1 and predatory mites for integrated pest mite control.

Abstract Single-cell whole-genome sequencing methods have undergone great improvements over the past decade. However, allele dropout, which means the inability to detect both alleles simultaneously in an individual diploid cell, largely restricts the application of these methods particularly for medical applications. Here, we develop a new single-cell whole-genome sequencing method based on third-generation sequencing (TGS) platform named Refresh-seq (restriction fragment ligation-based genome amplification and TGS). It is based on restriction endonuclease cutting and ligation strategy in which two alleles in an individual cell can be cut into equal fragments and tend to be amplified simultaneously. As a new single-cell long-read genome sequencing method, Refresh-seq features much lower allele dropout rate compared with SMOOTH-seq. Furthermore, we apply Refresh-seq to 688 sperm cells and 272 female haploid cells (secondary polar bodies and parthenogenetic oocytes) from F1 hybrid mice. We acquire high-resolution genetic map of mouse meiosis recombination at low sequencing depth and reveal the sexual dimorphism in meiotic crossovers. We also phase the structure variations (deletions and insertions) in sperm cells and female haploid cells with high precision. Refresh-seq shows great performance in screening aneuploid sperm cells and oocytes due to the low allele dropout rate and has great potential for medical applications such as preimplantation genetic diagnosis.

Abstract Background Triple-negative breast cancer (TNBC) is the most lethal subtype of breast cancer (BC). The circRNA-miRNA‒mRNA axis is a promising biomarker for the early diagnosis and prognosis of BC. However, the critical circRNA mediators involved in TNBC progression and the underlying regulatory mechanism involved remain largely unclear. Methods In this study, we carried out a circRNA microarray analysis of 6 TNBC patients and performed a gene ontology (GO) analysis. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was used to characterize important circRNAs involved in TNBC progression. The interaction between circRNAs and miRNAs was determined by dual luciferase and RNA immunoprecipitation (RIP) assays. Moreover, Transwell, wound healing and Cell Counting Kit-8 (CCK8) assays were performed with altered circRNA or miRNA expression in MDA-MB-231 and BT-549 cells to investigate the roles of these genes in cell invasion, migration and proliferation. Results A total of 78 circRNAs were differentially expressed in TNBC tissues, and the hsa_circ_0045881 level was significantly decreased in TNBC tissues and cells. Lentivirus-mediated hsa_circ_0045881 overexpression in MDA-MB-231 and BT-549 cells significantly reduced cell invasion and migration capacity. Additionally, hsa_circ_0045881 interacted with miR-214-3p in MDA-MB-231 cells. miR-214-3p mimics in MDA-MB-231 and BT-549 cells significantly enhanced cell invasion, migration and proliferation, but the other combinations of inhibitors had opposite effects on cell activity. Conclusions Our data indicated that the circRNA has_circ_0045881 plays key roles in TNBC progression and that hsa_circ_0045881 might act as a sponge for miR-214-3p to modulate its levels in TNBC cells, thereby regulating cell invasion, metastasis and proliferation. hsa_circ_004588 might be a potential prognostic marker and therapeutic target for TNBC.

High-entropy materials (HEMs) have attracted extensive attention in the field of electrochemical catalysis due to their unique properties. However, the preparation of high-entropy catalysts typically relies on high-temperature, energy-intensive, and time-consuming synthesis methods due to their compositional complexity. In this study, a facile low-temperature electrochemical reconstruction approach is adopted to synthesize Ag-decorated septenary Co-Cu-Fe-Mo-Zn-Ag-Ru high-entropy (oxy)hydroxide electrocatalysts for oxygen evolution reaction (OER). By introducing Ag and Ru elements and implanting Ag nanoparticles to co-regulate the electronic structure of the catalysts, the as-prepared catalyst achieves remarkable OER performance with a low overpotential of 298 mV at 100 mA/cm2 and a small Tafel slope of 30.1 mV/dec in 1 mol/L KOH. This work offers a valuable strategy for developing high-performance high-entropy OER electrocatalysts.

Identifying ecosystem service functions, clarifying the spatiotemporal trade-offs and synergies of terrestrial ecosystem services and their driving mechanisms, and exploring differentiated ecological functional zoning are crucial steps in achieving healthy regional ecosystem management, and are conducive to developing diversified ecological restoration strategies, establishing a robust cross-regional collaborative management mechanism, implementing differentiated ecological management strategies, and contributing to the construction of a Beautiful China. This paper, based on the InVEST model, Pearson correlation, GeoDetector, and Spatiotemporal Geographically Weighted Regression models, constructs a spatial quantification model of the trade-offs and synergies among five key ecosystem service functions—habitat quality, soil retention, water conservation, food supply, and carbon sequestration—of China’s terrestrial ecosystems from 2000 to 2020. It explores the influencing factors of terrestrial ecosystem services in China and their spatiotemporal heterogeneity, thereby investigating the future strategies for ecological functional zoning and management of China’s national land space. The results indicate that: (1) during 2000–2020, China’s food supply and soil conservation have increased. However, the habitat quality, water conservation, and carbon sequestration have decreased. (2) Significant spatial and temporal heterogeneities exist in the key ecosystem services of China’s terrestrial ecosystems. (3) Natural, economic, and social factors all impact China’s terrestrial key ecosystem services. Among them, slope, annual average precipitation, land development intensity, and vegetation coverage are the main influencing factors, and different factors exhibit significant spatial heterogeneity. (4) Significant trade-offs/synergy effects among critical terrestrial ecosystem services exist in China. (5) China’s national territory is divided into four ecological protection functional zones: ecological restoration areas, ecological control areas, resilient development areas, and ecological conservation areas, and explores differentiated zoning optimization control paths.

Abstract Intermittent hypoxia (IH) is a hallmark of obstructive sleep apnea (OSA), which is related to tumorigenesis and progression. We explored the possible mechanisms by which OSA may promote the development of non‐small cell lung cancer (NSCLC). In this study, NSCLC cells with and without miR‐106a‐5p inhibition were exposed to IH or room air (RA), and subsequently, exosomes were extracted and identified. Macrophages were incubated with these exosomes to detect the expression of the STAT3 signaling pathway and M2‐type macrophage markers, as well as the effect of the macrophages on the malignancy of NSCLC cells. A nude mouse tumorigenesis model was constructed to detect the effects of exosomal miR‐106a‐5p on M2 macrophage polarization and NSCLC cell malignancy. Our results showed that IH exosomes promoted the polarization of M2 macrophages, thereby promoting the proliferation, invasion, and metastasis of NSCLC cells. Further, Based on microarray analysis of RA and IH exosomes, we discovered that miR‐106a‐5p, transferred to the macrophages through exosomes, participated in this mechanism by promoting M2 macrophage polarization via down‐regulating PTEN and activating the STAT3 signaling pathway in vitro and in vivo. For patients with NSCLC and OSA, exosomal miR‐106a‐5p levels showed a positive relation to AHI. Exosomal miR‐106a‐5p represents a potential therapeutic target among patients with concomitant cancer and NSCLC.

Adprhl2 (OMIM: 610624) mutation associated stress-induced childhood-onset neurodegeneration with variable ataxia and seizures (CONDSIAS, OMIM: 618170) is a sporadic neurodegenerative disease with poor prognosis. ADPRHL2 encodes ADP-ribosylhydrolase 3 (ARH3), which participates in ADP-ribosylation to remove poly-ADP ribose (PAR). We found a new compound heterozygous mutation in the ADPRHL2 gene c.580C > T (p.Gln194Ter) and c.803-1G > A in a 30-month-old boy, who showed gait instability, abnormal EEG, and developmental delay after respiratory infection. He died of convulsions 4 months after onset. By constructing a mutant plasmid and using Western blot to detect the expression of ARH3 and PAR, it was demonstrated that the ADPRHL2 gene c.580C > T (p.Gln194Ter) and c.803-1G > A is pathogenic according to ACMG guidelines.

The restoring force model (RFM) is the basis of elasto-plastic time-history analysis and forms a critical part of the seismic response analysis of structures. Based on the previous research results of a novel modular steel-concrete composite column, this paper qualitatively processed the test data through dimensionless and regression analyses. A three-fold line skeleton curve model (SCM) suitable for modular steel-concrete composite columns was proposed, and the stiffness degradation and hysteretic rule of the novel modular steel-concrete composite column were presented. Based on these results, a three-fold line RFM considering the stiffness degradation was established. Comparing the calculated results of the resilience model with the test results showed that the yield point moment obtained from the test was 0.84−1.34 times the calculated moment of the RFM, with an average value (AVG) of 1.01 and a coefficient of variation (COV) of 0.02. The peak moment obtained from the test was 0.96−1.01 times the calculated moment of the RFM, with an AVG of 0.99 and a COV of 0.00. Thus, the reliability of the RFM of the novel modular steel-concrete composite column was verified.

Reactive oxygen species (ROS) are one of the potential molecules in response to a hypomagnetic field (HMF), and exposure to an HMF for eight weeks led to an increase in ROS levels in the whole hippocampus area in mice. ROS are mainly derived from the byproducts of mitochondrial metabolism. However, previous in vivo studies mostly focus on the influence of one time point of HMF exposure on the mouse hippocampus and lack comparative studies on the effects of different durations of HMF exposure on the mouse hippocampus. Here, we investigated the effects of different durations of HMF on the number of active mitochondria and ROS levels in mouse hippocampus. Compared with the geomagnetic field (GMF) group, we found that the number of active mitochondria in the hippocampus was significantly reduced during the sixth week of HMF exposure, whereas the number of active mitochondria was significantly reduced and the ROS levels was significantly increased during the eighth week of HMF exposure. The number of active mitochondria gradually decreased and ROS levels gradually increased in both GMF and HMF groups with prolonged exposure time. In addition, the expression level of the PGC-1α gene in the hippocampus, the main regulator of mitochondrial biogenesis, decreased significantly in the eighth week of HMF exposure. These results reveal that the changes in active mitochondria number and ROS levels were dependent on the durations of HMF exposure, and prolonged exposure to HMF exacerbates these changes.

Abstract In recent years, topological photonics inspired by electric topological insulators has promoted extensive research on robust electromagnetic (EM) wave manipulation and new wave‐functional devices. Optical resonators can significantly confine EM waves and are the basic building blocks for constructing diverse topological structures under a tight binding mechanism. As an artificial “magnetic atom,” the split‐ring‐resonator (SRR) is one of the most attractive optical resonators. SRRs provide an excellent and flexible platform for constructing various topological structures with complex coupling distributions, uncovering abundant topological properties, and innovating practical devices. Here, the realization and fundamental EM responses of the SRR are briefly introduced. Compared to conventional EM resonance elements, the coupling between SRRs depends not only on the coupling distance but also on the orientation angle of the slits. The recent achievements in various low‐dimensional photonic topological structures composed of SRRs are summarized. Furthermore, this review explains the underlying physical principles and discusses progress in topological devices with SRRs, including wireless power transfer, sensing, and switching. Finally, this review provides an overview of the future of SRR topological structures and their impact on the development of novel topological systems and devices.

Abstract Neural stem cells (NSCs) transplantation is an attractive and promising treatment strategy for spinal cord injury (SCI). Various pathological processes including the severe inflammatory cascade and difficulty in stable proliferation and differentiation of NSCs limit its application and translation. Here, a novel physico‐chemical bifunctional neural stem cells delivery system containing magnetic nanoparticles (MNPs and methylprednisolone (MP) is designed to repair SCI, the former regulates NSCs differentiation through magnetic mechanical stimulation in the chronic phase, while the latter alleviates inflammatory response in the acute phase. The delivery system releases MP to promote microglial M2 polarization, inhibit M1 polarization, and reduce neuronal apoptosis. Meanwhile, NSCs tend to differentiate into functional neurons with magnetic mechanical stimulation generated by MNPs in the static magnetic field, which is related to the activation of the PI3K/AKT/mTOR pathway. SCI mice achieve better functional recovery after receiving NSCs transplantation via physico‐chemical bifunctional delivery system, which has milder inflammation, higher number of M2 microglia, more functional neurons, and axonal regeneration. Together, this bifunctional NSCs delivery system combined physical mechanical stimulation and chemical drug therapy is demonstrated to be effective, which provides new treatment insights into clinical transformation of SCI repair.

BackgroundExcessive sodium and low potassium intake are involved in the development of hypertension. Growing evidence showed that the sodium-to-potassium ratio (Na/K) was significantly associated with blood pressure (BP). However, studies on the dose-response relationship of spot urinary Na/K ratio with hypertension and BP in the general population are scarce, especially in the Chinese population.Materials and methodsData from the post-intervention survey of the Shandong Ministry of Health Action on Salt and Hypertension (SMASH) project was analyzed. Associations between Na/K molar ratio and hypertension prevalence and between Na/K molar ratio and BP indices were analyzed using multivariable logistic and linear regression, respectively, followed by subgroup analysis and interaction analysis. The restricted cubic spline model was used to explore the dose–response relationship. Informed by existing literature, we adjusted for potential confounding factors, including temperature and renal function, to assess the association and dose–response relationship.ResultsThere was a non-linear positive association between Na/K and hypertension (OR:1.09, 95%CI: 1.08–1.11) and a linear positive association between Na/K and systolic BP, diastolic BP, and mean arterial pressure (β 0.53, 95%CI: 0.45–0.60; β 0.36, 95%CI: 0.31–0.41; and β 0.42, 95%CI: 0.36–0.47, respectively). The association was stronger in individuals with hypertension, female patients, those in the 50–59-year age group, and those who were obese. Environmental temperatures had little impact on associations.ConclusionOur findings provide further evidence that the spot urinary Na/K ratio is a simple, useful, and convenient indicator for monitoring salt reduction and potassium increase, which could be used in clinical and public health practices.

Abstract Background Different neural subtypes are selectively lost in diverse neurodegenerative diseases. Huntington’s disease (HD) is an inherited neurodegenerative disease characterized by motor abnormalities that primarily affect the striatum. The Huntingtin (HTT) mutation involves an expanded CAG repeat, leading to insoluble polyQ, which renders GABA+ medium spiny neurons (MSN) more venerable to cell death. Human pluripotent stem cells (hPSCs) technology allows for the construction of disease-specific models, providing valuable cellular models for studying pathogenesis, drug screening, and high-throughput analysis. Methods In this study, we established a method that allows for rapid and efficient generation of MSNs (> 90%) within 21 days from hPSC-derived neural progenitor cells, by introducing a specific combination of transcription factors. Results We efficiently induced several neural subtypes, in parallel, based on the same cell source, and revealed that, compared to other neural subtypes, MSNs exhibited higher polyQ aggregation propensity and overexpression toxicity, more severe dysfunction in BDNF/TrkB signaling, greater susceptibility to BDNF withdrawal, and more severe disturbances in nucleocytoplasmic transport (NCT). We further found that the nuclear lamina protein LMNB1 was greatly reduced in HD neurons and mislocalized to the cytoplasm and axons. Knockdown of HTT or treatment with KPT335, an orally selective inhibitor of nuclear export (SINE), effectively attenuated the pathological phenotypes and alleviated neuronal death caused by BDNF withdrawal. Conclusions This study thus establishes an effective method for obtaining MSNs and underscores the necessity of using high-purity MSNs to study HD pathogenesis, especially the MSN-selective vulnerability.

Abstract Action recognition has been applied in fields such as smart homes, gaming, traffic management, and security monitoring. Motion recognition is helpful for biomechanical analysis, auxiliary training systems, table tennis robots, motion-sensing games, virtual reality and other fields. In our study, we collected data on table tennis skill motion, created the TTMD6 dataset, and analyzed the characteristics of table tennis paddle trajectories. We propose a motion recognition algorithm to recognize paddle trajectories. Other research has used multijoint data to identify actions, while we use only the paddle trajectory to recognize table tennis skill motions, accelerating the speed of motion recognition. Therefore, it is feasible to use paddle trajectories to recognize table tennis skill motions.

Abstract Background Sarcopenia is a senile syndrome of age-related muscle loss. It is thought to affect the development of chronic kidney disease and has a serious impact on the quality of life of the elder adults. Little is known about the association between sarcopenia and new-onset chronic kidney disease in middle-aged and elder adults. Using nationally representative data from the China Health and Retirement Longitudinal Study (CHARLS), we conducted a longitudinal analysis to investigate the association between sarcopenia status and new-onset chronic kidney disease in middle-aged and elder adults in China. Methods The study population consisted of 3676 participants aged 45 or older selected from 2011 CHARLS database who had no history of chronic kidney disease at the baseline and completed the follow-up in 2015. A multivariate cox regression model was employed to examine the association between sarcopenia and the incidence of new-onset chronic kidney disease. Results Followed up for 4 years, a total of 873 (22.5%) new cases of chronic kidney disease occurred. Among them, participants diagnosed with sarcopenia (HR1.45; 95% CI 1.15–1.83) were more likely to develop new-onset chronic kidney disease than those without sarcopenia. Similarly, patients with sarcopenia were more likely to develop new-onset chronic kidney disease than those with possible sarcopenia (HR 1.27; 95%CI 1.00-1.60). Subgroup analysis revealed that elder adults aged between 60 and 75 years old (HR 1.666; 95%CI 1.20-22.28), with hypertension (HR 1.57; 95%CI 1.02–2.40), people with sarcopenia had a significantly higher risk of developing new-onset chronic kidney disease than those without sarcopenia (all P

Copper-based azide (Cu(N3)2 or CuN3, CA) chips synthesized by in-situ azide reaction and utilized in miniaturized explosive systems has become a hot research topic in recent years. However, the advantages of in-situ synthesis method, including small size and low dosage, bring about difficulties in quantitative analysis and differences in ignition capabilities of CA chips. The aim of present work is to develop a simplified quantitative analysis method for accurate and safe analysis of components in CA chips to evaluate and investigate the corresponding ignition ability. In this work, Cu(N3)2 and CuN3 components in CA chips were separated through dissolution and distillation by utilizing the difference in solubility and corresponding content was obtained by measuring N3− concentration through spectrophotometry. The spectrophotometry method was optimized by studying influencing factors and the recovery rate of different separation methods was studied, ensuring the accuracy and reproducibility of test results. The optimized method is linear in range from 1.0–25.0 mg/L, with a correlation coefficient R2 = 0.9998, which meets the requirements of CA chips with a milligram-level content test. Compared with the existing ICP method, component analysis results of CA chips obtained by spectrophotometry are closer to real component content in samples and have satisfactory accuracy. Moreover, as its application in miniaturized explosive systems, the ignition ability of CA chips with different component contents for direct ink writing CL-20 and the corresponding mechanism was studied. This study provided a basis and idea for the design and performance evaluation of CA chips in miniaturized explosive systems.