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This study aimed to investigate the effects of progressive postural control exercise (PPCE) vs core stability exercise (CSE) in patients with chronic low back pain (CLBP).A total of 34 young-adult participants with CLBP were randomly assigned to two groups (the PPCE group and the CSE group). They received instructions for two different exercise training regimens persisting over 8 weeks. Before, after, and at 6 months after the intervention, the participants were evaluated on the basis of pain intensity (VAS), degree of dysfunction (ODI and RMDQ), contractility of transversus abdominis (TrA) and lumbar multifidus (MF), as well as the ability to control static posture.There was no significant difference between the results of the PPCE group and the CSE group. At the 6-month follow-up after the 8-week treatment, the scores of VAS, ODI, and RMDQ in the two groups decreased significantly compared to before (p 0.05). The percentage change in thickness of bilateral TrA and left MF (p 0.05) was elevated and the sway area of center of pressure during static stance tasks with eyes opened (p 0.05) was decreased in both groups.In the short term, PPCE provides positive effects similar to those of core stability exercise in patients with CLBP. The effective mechanism of PPCE might be the consequence of neuromuscular plasticity and adaptation adjustments. PPCE enriches the choices of treatment for CLBP.The trial was registered at www.chictr.org.cn , identifier ChiCTR2100043113.Chronic low back pain (CLBP) is a widespread disorder with highly recurrent prevalence. As of now, the treatment effects are not satisfactory, leading to a search for novel therapies that might work better in patients with CLBP. This study comprehensively explored the effects of progressive postural control exercise, as compared to core stability exercise, on patients with CLBP. The outcomes included pain intensity, disability of daily life, contractility of trunk muscles, and postural control. The results of the study showed that the efficacy of exercises in patients in the experimental group was similar to that of the control group and both exercise treatments improved the pain intensity, the disability, the contractile function of trunk muscle, as well as postural control in patients with CLBP in the short term. The mechanism of the effects of progressive postural control exercise might be the consequence of “neuromuscular plasticity” and adaptation adjustments.

Fresh goji (Lycium barbarum L.) berries were treated with high-concentration (50% and 90%) oxygen shocking for 30 min and then stored at 0 ± 0.5 °C for 30 d. Decay, aerobic plate count, firmness, weight loss, total soluble solid (TSS), and titratable acidity (TA) were evaluated during storage. A total of 90% O2 shocking more effectively reduced decay and maintained the weight loss and firmness of goji berries. Subsequently, changes in fungi communities were analyzed using high-throughput sequencing (HTS) in the 90% O2-shocking and control groups. The results showed that 90% O2 shocking retained the richness and diversity of fungi communities and the microbiome was related to the quality properties of the fruit. Thus, we inferred that high oxygen shocking inhibited the development of natural decay and maintained the satisfying quality of goji berries by affecting the fungi community composition, which reduced the growth of pathogenic fungi and harmful saprotrophic fungi in the genera, such as Filobasidium sp., Alternaria sp., and Cladosporium sp. We provide a new insight into the disease development and quality changes during the storage of postharvest goji berries.

Diabetic retinopathy (DR) is still the fastest growing cause of blindness in working aged adults, and its typical characteristics are endothelial cell dysfunction and pericytes loss. Transcription factor fork head box P1 (FOXP1) is a member of FOX family involved in diabetes progression and is expressed in endothelial cells. The purpose of this study was to investigate the role and mechanism of FOXP1 in DR.The vitreous of DR patients and non-DR patients were collected, and the expression of FOXP1 was detected by real-time polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA). Human umbilical vein endothelial cells (HUVECs) cultured in high glucose simulated DR environment, and the expressions of FOXP1, vascular endothelial growth factor (VEGF), and pigment epithelium derived factor (PEDF) were detected by RT-qPCR and western blot (WB) after transfection of small interfering RNA (siRNA) to knock out FOXP1. At the same time, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MTT), 5-ethynyl-2'-deoxyuridine assay (EDU), flow cytometry, Transwell assay, and tube-forming experiment were performed to determine cell proliferation, migration, and tube-forming ability.We found that FOXP1 was highly expressed in the vitreous of DR patients and HUVECs under high glucose condition. After FOXP1 was decreased, the activation of VEGF expression and inhibition of PEDF expression in HUVECs induced by high glucose were reversed; meanwhile, cell proliferation, migration, and tube formation decreased, and apoptosis was promoted.Generally, FOXP1 is highly expressed in the vitreous of DR patients, and its silence prevented VEGF/PEDF signaling pathway stimulated by high glucose and also reduced the proliferation, migration, and tube formation of endothelial cell, thus improving vascular endothelial dysfunction caused by DR. The results indicate that FOXP1 may be a therapeutic target of DR.

Patients with chronic non-specific low back pain (CNSLBP) were found with impaired postural control in previous studies. Since the trunk muscle take important efforts on core stability, the study aimed to examine the relationships of postural control during stance tasks and the contractility of trunk muscle in young adults with CNSLBP and without.Healthy individuals (n = 25) and individuals with CNSLBP (n = 30) were included. The thickness of the bilateral transversus abdominis (TrA) and lumbar multifidus (MF) was measured during rest and maximal voluntary contraction, and the change percentages (TrA%, MF%) were calculated. Regarding postural control, COP path length and sway area during the stance tasks were measured thrice in each group.The bilateral TrA% of the CNSLBP group was less than that of the HC group (p 0.05). The bilateral TrA% of the CNSLBP group was less than that of the HC group (p 0.05). The bilateral MF% showed no significantly different(p 0.05) between the two groups. Compared with healthy controls, CNSLBP patients resulted larger path length and sway area of COP during most of static stance tasks. During the EO task in the CNSLBP group, TrA% was found correlate to COP path length (p 0.05); the right MF% was correlated with COP sway area (p 0.05). No significant correlations appeared in the healthy controls (p 0.05).Compared with healthy individuals, impaired postural control during static stance with eyes open in patients with CNSLBP was likely to be related to the poor contraction ability of bilateral transversus abdominis and correlated to the normal contraction ability of right lumbar multifidus.

BackgroundNon-specific chronic low back pain (NSCLBP) is a multifactor disorder with high prevalence rate of people all around the world. Lumbar paraspinal muscle (LPM) plays an important role of spine stabilization. However, the electrical properties of LPM in the patients were unclear. Aim to explore the electrical properties of bilateral LPM between patients with NSCLBP and healthy controls (HC), the study was designed to applied electrical impedance myography (EIM) technique to measure the LPM.MethodsA total of thirty participates (15 in NSCLBP group, 15 in HC group) were instructed to have each side of LPM measured at the rest state and the maximum volunteer contraction (MVC) state. Then measured the EIM parameters of LPM at L4/5 level by a multi-frequency device. Each measurement repeated three times. The EIM parameters at 50 kHz, 100 kHz, 200 kHz current frequencies were enrolled into the statistical analysis.ResultsAt the three current frequencies, parameters in the right side of LPM had not significantly difference from the left (P>0.05, respectively). Resistance (R) of LPM in NSCLBP group was larger than that in HC group (PPP>0.05, respectively). At 50 kHz, relationships between the EIM parameters and geometric parameters of LPM were not significant in NSCLBP group or HC group (P>0.05, respectively).ConclusionThe electrical properties of bilateral LPM in young adults with NSCLBP were different from those of healthy individuals regardless of any current frequency. The ultrasound parameters of LPM, however, showed no significant difference between young patients with NSCLBP and healthy participants. EIM measurement might be more sensitive than rehabilitative ultrasound for NSCLBP in young adults.Trial registrationThe Chinese Clinical trial registration number: ChiCTR2100043113.

It is crucial to address the dietary problems of hypertensive patients. The effect and mechanism of different contents of soybean protein on cooking quality and angiotensin-converting enzyme (ACE) inhibitory action in the extruded rice were firstly investigated. The results showed that the extruded rice with soybean protein possessed the higher taste value (90.32 ± 2.31), hardness (2.65 ± 0.01 g), and good pasting quality (p ≤ 0.05). Meanwhile, the soybean protein notably retarded the starch digestibility; the sample with 6% soybean protein showed the fewest rapidly digestible starch (RDS) content (78.82 ± 0.01 mg g−1) and the most slowly digestible starch (SDS) content (8.97 ± 0.45 mg g−1). Importantly, the ACE inhibition rate improved from 17.09 ± 0.01% to 74.02 ± 0.65% in the 6% soybean protein sample because of the production of peptides. The peptide composition of samples were compared, which showed that the effective ACE-inhibitory peptides usually contain 2~20 amino acids, and Pro, Leu, Ile, Val, Phe, and Ala were the main components. Overall, moderate soybean protein would give a good quality and lower ACE activity in extruded food.

Background/Aim: Music-based therapy plays a role in central nervous system diseases. We aimed to explore the effect of different doses and durations of music therapy on motor function recovery after stroke and the underlying molecular mechanisms.Methods: Adult male Sprague–Dawley rats were subjected to middle cerebral artery occlusion (MCAO) for 1 h, which was followed by reperfusion. In experiment 1, the rats that survived 1 week after MCAO surgery were randomly allocated into four groups (n = 10 per group): MCAO group, 1 h music group (Mozart K.448 music therapy 1 h per day for 2 weeks), 12 h music group (Mozart K.448 music therapy 12 h/day for 2 weeks), and accelerated music group (reversely accelerated music therapy 12 h for 2 weeks, AM group). In experiment 2, the survived rats were randomly divied into three groups: MCAO group, 12 h music group (music therapy 12 h/day for 3 weeks), and 12 h music-R group (music therapy 12 h/day for 2 weeks and rest for 1 week). Three neuroscores were evaluated daily, starting on the first day after surgery until the end of the experiment. The rats were killed 3 weeks after MCAO surgery in experiment 1 or 4 weeks after surgery in experiment 2. Nissl staining of infart core, peri-infarct zone, and motor cortex was performed to assess neuronal survival and regeneration. Western blot and immunofluorescence were used to detect the expression and distribution of brain-derived neurotrophic factor (BDNF) and glial fibrillary acidic protein (GFAP) in ipsilateral hemispheres.Results: In the experiment of different music therapy doses, the motor function in the 12-h music group but not in the 1-h music group and AM group was significantly improved compared with that of the MCAO group. The BDNF protein level of the ipsilateral hemisphere motor cortex in the 12-h music group and the 1-h music group was higher than that of the MCAO group. The neurons and Nissl bodies were more in the 12-h music group than in the MCAO group. Immunofluorescence assay showed that a 12 h music therapy induces BDNF and GFAP accumulation at the damage boundary. In the experiment of different music therapy durations, 3 weeks music therapy (12 h music group) induced more longer cell synapses and more clearer cell-to-cell connections than 2 weeks music intervention (12 h music-R group). Moreover, the GFAP morphology in the 12-h music group was more similar to mature activated astrocytes than that in the 12-h music-R group.Conclusions: Music therapy may improve poststroke motor function and promote neuronal repair in the long term. The mechanism may be through stimulating BDNF and GFAP secretion in the injured motor cortex.

Background: Although anterior cruciate ligament reconstruction (ACLR) can restore the stability and function of the knee joint, patellofemoral joint cartilage damage still progresses. Currently, the clinically important factors that lead to the progression of patellofemoral articular cartilage damage are not fully understood. Purpose: To investigate the factors that affect the progression of patellofemoral articular cartilage damage after ACLR. Study Design: Cohort study; Level of evidence, 2. Methods: Among 160 patients who underwent ACLR between January 2015 and December 2019, the authors evaluated 129 patients for at least 1 year after surgery. Within 1 week before ACLR and at the last follow-up, patients underwent subjective functional assessment and magnetic resonance imaging evaluations of articular cartilage damage (modified Outerbridge assessment). At the last follow-up, the side-to-side difference on KT-2000 arthrometer and bilateral quadriceps muscle strength were measured. Univariate and multivariate logistic regression analyses were performed. Results: The mean follow-up was 24.69 ± 10.74 months. Progression of patellar cartilage damage from preoperatively to final follow-up was seen in 45 patients ( P < .001). Logistic regression analysis revealed that the follow-up period ( P = .047; odds radio (OR) = 0.953) (improvement of patellar cartilage damage with longer follow-up), partial lateral meniscal resection ( P = .004; OR = 6.929), partial medial meniscal resection ( P = .004; OR = 6.032), and quadriceps muscle strength Conclusion: Cartilage damage at the patellofemoral joint, especially the patellar cartilage, still progresses after ACLR. At a mean follow-up of 24.69 months after ACLR, partial meniscal resection and quadriceps femoris muscle strength were found to be the main risk factors for the progression of patellofemoral articular cartilage damage after ACLR.

There are few studies on the genetic changes of meniscus degeneration. We used anterior cruciate ligament resection of Wuzhishan pig to prepare a meniscus degeneration model, and applied gene chip technology to detect differentially expressed genes in degenerative meniscus tissue. Then we applied GO analysis, Pathway analysis, Core gene network analysis and Relevant miRNAs analysis to discover relevant regulatory networks of meniscus degeneration. As a result, we detected 893 differentially expressed genes, mainly involving hormone, apoptosis, inflammation and other mechanisms, and obtained MUC13, Inflammatory mediator regulation of TRP channels, MDFI, mir-335-5p and so on that may play a key role. In summary, we have established a reliable animal model of meniscus degeneration and found that meniscus degeneration involves several possible molecular mechanisms, which will provide molecular targets for further research of the disease in the future.

Synovitis in osteoarthritis (OA) is a very common condition. However, its underlying mechanism is still not well understood. This study aimed to explore the molecular mechanisms of synovitis in OA. The gene expression profile GSE82107 (downloaded from the Gene Expression Omnibus database) included 10 synovial tissues of the OA patients and 7 synovial tissues of healthy people. Subsequently, differentially expressed gene (DEG) analysis, GO (gene ontology) enrichment analysis, pathway analysis, pathway network analysis, and gene signal network analysis were performed using Gene-Cloud of Biotechnology Information (GCBI). A total of 1,941 DEGs consisting of 1,471 upregulated genes and 470 downregulated genes were determined. Genes such as PSMG3, LRP12 MIA-RAB4B, ETHE1, SFXN1, DAZAP1, RABEP2, and C9orf16 were significantly regulated in synovitis of OA. In particular, the MAPK signalling pathway, apoptosis, and pathways in cancer played the most important roles in the pathway network. The relationships between these pathways were also analysed. Genes such as NRAS, SPHK2, FOS, CXCR4, PLD1, GNAI2, and PLA2G4F were strongly implicated in synovitis of OA. In summary, this study indicated that several molecular mechanisms were implicated in the development and progression of synovitis in OA, thus improving our understanding of OA and offering molecular targets for future therapeutic advances.

In this study, rotating electroosmotic flow of two immiscible fluids in a microparallel channel is investigated. The electric double layer (EDL) potential distribution is considered by using linear Poisson–Boltzmann (P-B) equation. Based upon the analytical charge density distribution, an analytical solution of flow velocity can be obtained by solving the modified Navier–Stokes (N-S) equation in the rotating frame. Besides, the equilibrium condition of stress, including shear stress and Maxwell stress, is taken into account as boundary conditions at the interface to analyze the distribution of the two-layer fluids velocity. It is found that, due to the rotational effect of the microchannel, the Coriolis force can generate a (secondary) transverse flow in horizontal direction perpendicular to the mainstream direction and the velocity amplitude of two-layer fluids has a reduced flow along the mainstream direction. In addition, the results indicate that double fluid velocity distribution is strongly influenced by several non-dimensional parameters, such as the dielectric constant ratio e, density ratio ρ, viscosity ratio μ of the two layer fluids, rotating angular velocity ω, interface zeta potential difference Δφ*, interface charge density jump Q, the normalized thickness of two layer Newtonian fluids h1*, h2* and electrokinetic width K1, K2. Smaller μ and larger e, Δφ* lead to larger velocity amplitude for lower fluid (noted as fluid II), but an opposite trend can be found for upper fluid (namely fluid I). The increase of Q or decrease of ρ leads to the increases of velocity amplitude for both fluid I and fluid II. Furthermore, interestingly, we observe that the mainstream speed at the interface does not depend on the interface zeta potential difference Δφ*, the dielectric constant ratio e and electrokinetic width K2 of fluid II when the depths of h1* and h2* are identical.

Space electroosmotic thrusters (EOTs) are theoretically investigated in a soft charged nanochannel with a dense polyelectrolyte layer (PEL), which is considered to be more realistic than a low-density PEL. When the PEL is dense, its permittivity is smaller than the one of the electrolyte solution layer, leading to rearrangement of ions in the channel, which is denoted as the ion partitioning effect. It is noted that fluid viscosity becomes high within the PEL owing to the hydration effect. An analytical solution for electroosmotic velocity through the channel is obtained by utilizing the Debye–Hückel linearization assumption. Based on the fluid motion, thruster performances, including thrust, specific impulse, thrust-to-power ratio, and efficiency, are calculated. The ion partitioning effect leads to enhancement of the thruster velocity, while increase of the dynamic viscosity inside the PEL reduces the flow rate of the fluid. Therefore, these performances are further impacted by the dense soft material, which are discussed in detail. Moreover, changes or improvements of the thruster performances from the dense PEL to the weak PEL are presented and compared, and distributions of various energy items are also provided in this study. There is a good result whereby the increase in electric double layer thickness promotes the development of thruster performances. Ultimately, the simulated EOTs produce thrust of about 0 to 20 μN and achieve thruster efficiency of 90.40%, while maintaining an appropriate thrust–power ratio of about 1.53 mN/W by optimizing all design parameters.

Enzymatic browning is one of the major difficulties for the preservation and commercial value of fresh-cut products. To research more healthy and inexpensive anti-browning methods, we investigated the effect of ultrasonic coupling purslane extract on the browning resistance of fresh-cut potato during 8d storage at 4 °C. Firstly, the optimal ultrasonic time (10 min) was obtained. Then, the results showed that the combined application with lower purslane extract concentration (0.02%, w/w) could achieve a better anti-browning effect than the optimal concentration of alone purslane extract (0.05%, w/w). The combined application not only significantly inhibited the key enzyme activities of polyphenol oxidase (PPO) and peroxidase (POD), but also effectively reduced the damage to cell membrane, maintained its integrity and permeability. Meanwhile, it also improved antioxidant capacity during storage. Overall, the ultrasonic cavitation combined with purslane extract would be a promising method for fresh-cut industry.

// Runhua Lin 1, * , Chong Zhang 1, * , Jiaxuan Zheng 1, * , Dongping Tian 1 , Zhijin Lei 1 , Donglin Chen 1 , Zexin Xu 1 , Min Su 1 1 Institute of Clinical Pathology, Guangdong Provincial Key Laboratory of Infectious Disease and Molecular Immunopathology, Shantou University Medical College, Shantou, Guangdong, 515031, PR China * These authors contributed equally to this work Correspondence to: Min Su, e-mail: minsu@stu.edu.cn Keywords: esophageal carcinogenesis, chronic inflammation, oxidative DNA damage, DNA double-strand breaks, genomic instability Received: November 16, 2015 Accepted: March 06, 2016 Published: March 25, 2016 ABSTRACT Chronic inflammation is associated with increased risk of cancer development, whereas the link between chronic inflammation and esophageal carcinogenesis is still obscure heretofore. This study aimed to investigate the relationship between chronic inflammation and DNA damage, as well as the possible role of DNA damage in esophageal carcinogenic process. Endoscopic esophageal biopsies from 109 individuals from Chaoshan littoral, a high-risk region for esophageal squamous cell carcinoma (ESCC), were examined to evaluate the association between chronic inflammation and histological severity, while additional 204 esophageal non-tumor samples from patients with ESCC were collected. Immunohistochemistry was performed to detect the oxidative DNA damage and DNA double-strand breaks (DSBs). Significantly positive correlation was observed between degree of chronic inflammation and esophageal precursor lesions ( r s = 0.37, P < 0.01). Immunohistochemical analysis showed that oxidative DNA damage level was positively correlated with the degree of chronic inflammation ( r s = 0.21, P < 0.05). Moreover, the level of oxidative DNA damage positively correlated with histological severity ( r s = 0.49, P < 0.01). We found that the extent of DSBs was progressively increased with inflammation degree ( P < 0.01) and the progression of precancerous lesions ( P < 0.001). Collectively, these findings provide evidence linking chronic inflammation-associated genomic instability with esophageal carcinogenesis and suggest possibilities for early detection and intervention of esophageal carcinogenesis.

In this study, P. kudriavzevii was isolated and identified as an effective antagonistic yeast, which could significantly inhibit the rotting rate, weight loss, and delay the color change, with no effect on total soluble solids (TSS), titratable acid (TA), or firmness during cherry tomato storage. High-throughput sequencing was used to survey the effect of P. kudriavzevii on fungal community throughout cold storage. The results showed that the biological succession of predominant pathogens was disrupted by P. kudriavzevii. The abundance of Botrytis and Alternaria was higher in the control than upon P. kudriavzevii treatment at 28 d, but some yeast genera such as Naganishia, Wickerhamomyces, and Cutaneotrichosporon at 14 d, Pichia and Sporidiobolus at 21 d, and Cystofilobasidium at 28 d, had relatively higher abundances in P. kudriavzevii treatments than the control. Oddly, as an antagonist agent, P. kudriavzevii was not the dominant population, indicating that altering the course of succession of the fungal community may be an effective mechanism of antagonistic yeast. Furthermore, the total network correlation analysis of fungal community revealed that the community development was more dependent on similarities in function than on taxonomic relationships.

The surface browning usually occurs on fresh-cut potato during storage. The effect of short-time high oxygen pretreatment on anti-browning of fresh-cut potato slices was investigated. The whole potato tubers were firstly immersed in the oxygen concentration of 21%, 60% and 80% for 20 min. Then, the potatoes were peeled, cut and stored at 4 °C for 8 days. The results showed that the short-time 80% oxygen pretreatment possessed significantly anti-browning effect by retarding the increase of polyphenol oxidase (PPO) activity and the accumulation of malondialdehyde (MDA) content, maintaining the cell integrity. Meanwhile, the 80% oxygen treatment could increase the activities of phenylalanine ammonia lyase (PAL) and peroxidase (POD), and the total phenolic content. Importantly, the 80% oxygen treatment could effectively improve the antioxidant capacity. Overall, all results suggest that the short-time high oxygen pretreatment holds great promise on anti-browning of fresh-cut potato.

Background & Aims Esophageal squamous cell carcinoma (ESCC) is the most common subtype of esophageal cancer. Little is known about the genetic changes that occur in esophageal cells during the development of ESCC. We performed next-generation sequence analyses of esophageal nontumor, intraepithelial neoplasia (IEN), and ESCC tissues from the same patients to track genetic changes during tumor development. Methods We performed whole-genome, whole-exome, or targeted sequence analyses of 227 esophageal tissue samples from 70 patients with ESCC undergoing resection at Shantou University Medical College in China from 2012 through 2015 (no patients had received chemotherapy or radiation therapy); we analyzed normal tissues, tissues with simple hyperplasia, dysplastic tissues (IEN), and ESCC tissues collected from different regions of the esophagus at the same time. We also obtained 1191 nontumor esophageal biopsy specimens from the Chaoshan region (a high-risk region for ESCC) of China (a high-risk region for ESCC) and performed immunohistochemical and histologic analyses to detect inflammation. Results IEN and ESCC tissues had similar mutations and copy number alterations, at similar frequencies; these differed from mutations detected in tissues with simple hyperplasia. IEN tissues had mutations associated with apolipoprotein B messenger RNA editing enzyme, catalytic polypeptide-like–mediated mutagenesis (a DNA damage mutational signature). Genetic analyses indicated that most ESCCs were formed from early stage IEN clones. Trunk mutations (mutations shared by >10% of paired IEN and ESCC tissues) were in genes that regulate DNA repair and cell apoptosis, proliferation and adhesion. Mutations in TP53 and CDKN2A and copy number alterations in 11q (contains CCND1 ), 3q (contains SOX2 ), 2q (contains NFE2L2 ), and 9p (contains CDKN2A ) were considered to be trunk variants; these were dominant mutations detected at high frequencies in clones of paired IEN and ESCC samples. In the esophageal biopsy samples from high-risk individuals (residing in the Chaoshan region), 68.9% had an evidence of chronic inflammation; the level of inflammation was correlated with atypical cell structures and markers of DNA damage. Conclusions We analyzed mutations and gene copy number changes in nontumor, IEN, and ESCC samples, collected from 70 patients. IEN and ESCCs each had similar mutations and markers of genomic instability, including apolipoprotein B messenger RNA editing enzyme, catalytic polypeptide-like. Genomic changes observed in precancerous lesions might be used to identify patients at risk for ESCC.

Currently, with the rapid development of many cities, water problems, such as water logging and water quality deterioration, occur inevitably. Thus, sponge city construction and low impact development (LID) utilization have become more important worldwide. However, previous works have failed to address the problem of selecting an optimal LID measure layout scenario by simultaneously considering various evaluation indices without subjective factors. In this study, we applied a new and outstanding statistical classifier, random forest, to aid in addressing this conundrum. It was tested on a case study in LiWan district, Guangzhou city. The following conclusions were drawn. (1) To some extent, LID measures are capable of reducing water discharge and generation of pollutants. (2) Excluding subjective factors, random forest can select an optimal LID measure layout scenario when simultaneously considering multiple indices. This study proposed a novel and effective means to evaluate the hydrologic effects of LID measures when constructing sponge cities and provided a guide for optimizing LID layouts.