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Objective The study objective was to determine whether mini-invasive transthoracoscopic atrial fibrillation ablation can delay the progression of atrial fibrillation from paroxysmal to persistent. Methods Patients aged 18 to 80 years with paroxysmal nonvalvular atrial fibrillation and a history of stroke or systemic thromboembolism were consecutively enrolled from September 2014 to June 2019. In the treatment group, patients underwent transthoracoscopic atrial fibrillation ablation plus left atrial appendage excision (atrial fibrillation ablation plus left atrial appendage excision group). Patients unwilling to receive surgical intervention were treated with antiarrhythmic drugs and oral anticoagulants and recruited as a control group (atrial fibrillation plus antiarrhythmic drugs group). The primary end point was the progression of atrial fibrillation from paroxysmal to persistent. Results This study included 49 patients in the atrial fibrillation plus antiarrhythmic drugs group (29 men) and 77 patients in the atrial fibrillation ablation plus left atrial appendage excision group (48 men). In the atrial fibrillation ablation plus left atrial appendage excision group, after a median follow-up of 951 days (interquartile range, 529-1366 days), 8 patients (10.4%) progressed to persistent atrial fibrillation. In the atrial fibrillation plus antiarrhythmic drugs group, after a median follow-up of 835 days (interquartile range, 548-1214 days), 14 patients (28.6%) progressed to persistent atrial fibrillation. The atrial fibrillation ablation plus left atrial appendage excision group had a significantly lower incidence of atrial fibrillation progression than the atrial fibrillation plus antiarrhythmic drugs group during follow-up (3.9 vs 12.3 per 100 person-years, log-rank 8.6, P = .003). Conclusions Patients with paroxysmal nonvalvular atrial fibrillation who chose to undergo transthoracoscopic atrial fibrillation ablation had a lower incidence of progression to persistent atrial fibrillation than patients who chose conservative therapy. This strategy might be especially suitable for patients with paroxysmal nonvalvular atrial fibrillation at high risk of stroke and high risk of bleeding.

One key risk factor of aortic valve stenosis in clinical practice is bicuspid aortic valve (BAV). Increasing evidence indicates that numerous microRNAs (miRs/miRNAs) are involved in BAV calcification via their target genes. miR‑330‑3p was found to be involved in the deterioration of BAV calcification by miR profiling in human calcified BAV and tricuspid aortic valve (TAV) tissues in the present study and the underlying mechanism was investigated. RNA sequencing was performed on four BAV and four TAV tissues from patients with aortic stenosis before these leaflets were examined for the expression levels of miR‑330‑3p and CREB‑binding protein (CREBBP) by reverse transcription‑PCR. The alteration of functional factors associated with calcification was also assessed by Western blotting and immunohistochemistry in human aortic tissue samples. The putative target of miR‑330‑3p was detected by dual‑luciferase assay in 293 cells. Furthermore, the influence of miR‑330‑3p expression on osteogenic progression was explored in cultured porcine valve interstitial cells (VICs). Rescue experiments of CRBBP were performed to confirm the influence of the miR‑330‑3p‑CREBBP pathway in the calcification progress in porcine VICs. RNA sequencing indicated distinct expression of miR‑330‑3p in human BAV tissues compared with TAV, which was then confirmed by PCR. CREBBP expression levels in human BAV and TAV leaflets also demonstrated the opposite alterations. This negative correlation was then confirmed in cultured porcine VICs. Under an osteogenic environment, cellular calcification was promoted in miR‑330‑3p‑overexpressed porcine VICs expressing higher bone morphogenetic protein 2, Runt‑related transcription factor 2, matrix metalloproteinase (MMP)‑2, MMP‑9 and collagen I compared with controls. Rescue experiments further confirmed that miR‑330‑3p played its role via targeting CREBBP in porcine VICs. Collectively, miR‑330‑3p was upregulated in calcified BAV compared with TAV. The upregulation of miR‑330‑3p promotes the calcification progress partially via targeting CREBBP.

Camera-equipped mobile devices are encouraging people to take more photos and the development and growth of social networks is making it increasingly popular to share photos online. When objects appear in overlapping Fields Of View (FOV), this means that they are drawing much attention and thus indicates their popularity. Successfully discovering and locating these objects can be very useful for many applications, such as criminal investigations, event summaries, and crowdsourcing-based Geographical Information Systems (GIS). Existing methods require either prior knowledge of the environment or intentional photographing. In this paper, we propose a seamless approach called “Spotlight”, which performs passive localization using crowdsourced photos. Using a graph-based model, we combine object images across multiple camera views. Within each set of combined object images, a photographing map is built on which object localization is performed using plane geometry. We evaluate the system's localization accuracy using photos taken in various scenarios, with the results showing our approach to be effective for passive object localization and to achieve a high level of accuracy.

Background: A patent false lumen (FL) in patients with thoracic endovascular aortic repair (TEVAR)-treated type B aortic dissection (TBAD) can cause a significant risk for late aortic expansion (LAE). We hypothesize that preoperative features can predict the occurrence of LAE. Methods: Sufficient preoperative and postoperative follow-up clinical and imaging feature data for patients treated with TEVAR in the First Affiliated Hospital of Nanjing Medical University from January 2018 to December 2020 were collected. A univariate analysis and multivariable logistic regression analysis were used to find potential risk factors of LAE. Results: Ninety-six patients were finally included in this study. The mean age was 54.5 ± 11.7 years and 85 (88.5%) were male. LAE occurred in 15 (15.6%) of 96 patients after TEVAR. Two preoperative factors showed strong associations with LAE according to the multivariable logistic regression analysis: preoperative partial thrombosis of the FL (OR = 10.989 [2.295–48.403]; p = 0.002) and the maximum descending aortic diameter (OR = 1.385 [1.100–1.743] per mm increase; p = 0.006). Conclusions: Preoperative partial thrombosis of the FL and an increase in the maximum aortic diameter are strongly associated with late aortic expansion. Additional interventions of the FL may help to improve the prognosis of patients with the high risk of late aortic expansion.

In this paper we develop a new sixth-order finite difference central weighted essentially non-oscillatory (WENO) scheme with Z-type nonlinear weights for nonlinear degenerate parabolic equations. The centered polynomial is introduced for the WENO reconstruction in order to avoid the negative linear weights. We choose the Z-type nonlinear weights based on the $L^2$-norm smoothness indicators, yielding the new WENO scheme with more accurate resolution. It is also confirmed that the proposed central WENO scheme with the devised nonlinear weights achieves sixth order accuracy in smooth regions. One- and two-dimensional numerical examples are presented to demonstrate the improved performance of the proposed central WENO scheme.

With the development of multimedia and computer graphics technologies, virtual reality (VR) is attracting more and more attention from both the academic communities and industrial companies. A head-mounted display (HMD) is the core equipment of VR. It envelops the entire sight of the wearer and reacts to some specific actions, mainly the head movement. Different from common video watching or game playing, VR poses the strict requirement of immersion so interaction methods need to be carefully designed. The hotword-based interaction as a typical hands-free method is very suitable for VR scenarios. However, the traditional hotword detection methods use a microphone to permit audio signal analysis. They not only incur significant recording overheads but are also susceptible to the surrounding noises. Instead of using the audio signals, we propose a motion-based hotword detection method called Alohomora. A multivariate time series (MTS) classification is formulated for processing the sensor data from multiple dimensions and types of motion sensors. We use a word extraction method for extracting and selecting patterns from MTS of motion data. Then, a classification model is trained using those discriminative patterns and finally the hotword can be detected in time. Alohomora is purely based on the motion sensors in HMDs without using any extra components such as microphone. As head tracking is always necessary in VR applications themselves, the overhead of Alohomora is nearly negligible. Finally, through extensive experiments, the final detection accuracy of Alohomora can exceed 90%.

Video streaming takes up an increasing proportion of network traffic nowadays. Dynamic adaptive streaming over HTTP (DASH) becomes the de facto standard of video streaming and it is adopted by Youtube, Netflix, and so on. Despite of the popularity, network traffic during video streaming shows an identifiable pattern which brings threat to user privacy. In this paper, we propose a video identification method using network traffic while streaming. Though there is bitrate adaptation in DASH streaming, we observe that the video bitrate trend remains relatively stable because of the widely used variable bit-rate (VBR) encoding. Accordingly, we design a robust video feature extraction method for eavesdropped video streaming traffic. Meanwhile, we design a VBR-based video fingerprinting method for candidate video set which can be built using downloaded video files. Finally, we propose an efficient partial matching method for computing similarities between video fingerprints and streaming traces to derive video identities. We evaluate our attack method in different scenarios for various video content, segment lengths, and quality levels. The experimental results show that the identification accuracy can reach up to 90% using only three-minute continuous network traffic eavesdropping.

Calcification of the bicuspid aortic valve (BAV) involves differential expression of various RNA genes, which is achieved through complex regulatory networks that are controlled in part by transcription factors and microRNAs. We previously found that miR-195-5p regulates the osteogenic differentiation of valvular interstitial cells (VICs) by targeting the TGF-β pathway. However, the transcriptional regulation of miR-195-5p in calcified BAV patients is not yet clear. In this study, stenotic aortic valve tissues from patients with BAVs and tricuspid aortic valves (TAVs) were collected. Candidate transcription factors of miR-195-5p were predicted by bioinformatics analysis and tested in diseased valves and in male porcine VICs. SP2 gene expression and the corresponding protein levels in BAV were significantly lower than those in TAV, and a low SP2 expression level environment in VICs resulted in remarkable increases in RNA expression levels of RUNX2, BMP2, collagen 1, MMP2, and MMP9 and the corresponding proteins. ChIP assays revealed that SP2 directly bound to the transcription promoter region of miR-195-5p. Cotransfection of SP2 shRNA and a miR-195-5p mimic in porcine VICs demonstrated that SP2 repressed SMAD7 expression via miR-195-5p, while knockdown of SP2 increased the mRNA expression of SMAD7 and the corresponding protein and attenuated Smad 2/3 expression. Immunofluorescence staining of diseased valves confirmed that the functional proteins of osteogenesis differentiation, including RUNX2, BMP2, collagen 1, and osteocalcin, were overexpressed in BAVs. In Conclusion, the transcription factor Sp2 is expressed at low levels in VICs from BAV patients, which has a negative impact on miR-195-5p expression by binding its promoter region and partially promotes calcification through a SMAD-dependent pathway.