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Pickering nanoemulsions were prepared using dual-protein natural symbiotic particles and their physicochemical stability was analyzed to broaden their potential application in the food industry. This study employed alkali extraction-isoelectric point precipitation (AE) and salt extraction-dialysis (SE) to enrich soy protein and flaxseed protein from soybean-flaxseed mixed meal for fabricating dual-protein symbiotic natural particles (SFNPs). First, two dual-protein symbiotic particles were characterized using appearance observation, AFM, circular dichroism, and Raman spectroscopy. Then, Pickering nanoemulsions were prepared with them as particle stabilizers and characterized for their physical and chemical stability. Results demonstrated that AE-SFNPs had a higher uniformity of size and better dispersibility. AE-SFNPs also showed a higher proportion (as high as 51.25%) of β-sheet in its secondary structure, endowing it a more tightly ordered and flexible structure compared with SE-SFNPs. Moreover, both AE-SFNPs and SE-SFNPs could be used to generate Pickering nanoemulsion. During 28 days of storage, the particle size of the stabilized emulsion of AE-SFNPs increased only about 14 nm and its ζ-potential was stabilized at about -34 mV. In contrast, the particle size of the stabilized emulsion of SE-SFNPs increased about 12 fold, and its ζ-potential was decreased 1.32 fold. The stability index (TSI, 5.83) of the AE-SFNPs was much smaller than the TSI value (55.13) of the SE-SFNPs when stored until the 28th day. Notably, the Pickering nanoemulsion stabilized by AE-SFNPs exhibited higher physical and chemical stability than that prepared by sodium caseinate (a common emulsifier used in food processing). Therefore, AE-SFNPs can serve as a natural and efficient plant protein-based particle stabilizer to prepare Pickering nanoemulsion. This work provides a new strategy to enhance the industrial application of nanoemulsions.

Tobacco consumption in China remains the primary cause of preventable mortality, with Shanghai being particularly affected by issues related to secondhand smoke exposure. This study explores the role of the public service hotline 12345, a grassroots initiative in Shanghai, in capturing public sentiment and assessing the effectiveness of anti-smoking regulations. Our research aims to accurately and deeply understand the implementation and feedback of smoking control policies: by identifying high-frequency points and prominent issues in smoking control work based on the smoking control work order data received by the health hotline 12320. The results of this study will assist government enforcement agencies in improving smoking monitoring and clarify the direction for improving smoking control measures. Text-mining techniques were employed to analyze a dataset comprising 78011 call sheets, all related to tobacco control and collected from the hotline between 1 January 2015 and 31 December 2019. This methodological approach aims to uncover prevalent themes and sentiments in the public discourse on smoking and its regulation, as reflected in the hotline interactions. Our study identified hotspots and the issues of greatest concern to citizens. Additionally, it provided recommendations to enforcement agencies to enhance their capabilities, optimize the allocation of human resources for smoking control monitoring, reduce enforcement costs and support for anti-smoking campaigns, thereby contributing to more effective tobacco control policies in the region.

Abstract Soft robots spark a revolution in human–machine interaction. However, developing high‐performance soft actuators remains challenging due to trade‐offs among output force, driving distance, control precision, safety, and compliance. Here, addressing the lack of long‐distance, high‐precision flexible linear actuators, an innovative pneumatic flexible linear actuator (PFLA) is introduced, inspired by the smooth and controlled process observed in snakes ingesting sizable food, such as eggs. This PFLA combines a soft tube, emulating the snake's body cavity, with a pneumatically driven piston. Through the joint modulation of moving resistance and driving force by pneumatic pressure, the PFLA exhibits exceptional motion control capabilities, including self‐holding without pressure supply, smooth low‐speed motion (down to 0.004 m s–1), high‐speed motion (up to 5.6 m s–1) with low air pressure demand, and a self‐protection mechanism. Highlighting its adaptability and versatility, the PFLA finds applications in various settings, including a wearable assistive devices, a manipulator capable of precise path tracking and positioning, and rapid transportation in diverse environments for pipeline inspection and firefighting. This PFLA combines biomimetic principles with sophisticated fluidic actuation to achieve long‐distance, flexible, precise, and safe actuation, offering a more adaptive solution for force/motion transmission, particularly in challenging environments.

The objective of this study was to investigate the effect of ultrasonic-assisted marination on the mass transfer kinetics and quality of duck breast meat and thigh meat. Results showed that the increase of ultrasonic power greatly accelerated the transfer of moisture and NaCl, and the highest yield was obtained by ultrasonic power of 450 W. The values of the mass transfer kinetics parameter (k2) for weight changes improved as the ultrasonic power increased. The application of ultrasound treatment enhanced the NaCl effective diffusion coefficients (De) of duck breast and thigh meat from 0.7889−0.9472 × 10−9 m2/s to 1.2661−1.3775 × 10−9 m2/s and the highest De was found with 450 W. The treatment of ultrasound can reduce shear force and water loss of duck samples. According to the analysis of water distribution, ultrasound could decrease the T22 values which indicated a decrease in water mobility. Thus, ultrasonic-assisted marination could be employed as an emerging technology for various meat-curing processes.

Abstract Acceptance of artificial pelleted diets contributes to increasing the cultured areas and output of carnivorous fish. However, the mechanism of acceptance of artificial pelleted diets remains largely unknown. In this study, the easy acceptance of artificial pelleted diets (EAD) group and the not easy acceptance of artificial pelleted diets (NAD) group of Largemouth bass (Micropterus salmoides) were divided based on the ratios of stomach weight/body weight (SB) after 0.5 h feeding, which was bigger than 18% in the EAD group and ranged from 8 to 12% in the NAD group. Through transcriptome and proteome sequencing, a total of 2463 differentially expressed genes (DEGs) and 230 differentially expressed proteins (DEPs) were identified, respectively. Integrated analyses of transcriptome and proteome data revealed that 152 DEPs were matched with the corresponding DEGs (named co-DEGs-DEPs), and 54 co-DEGs-DEPs were enriched in 16 KEGG pathways, including the metabolic pathways, steroid biosynthesis, fatty acid biosynthesis, etc. Furthermore, 3 terpenoid backbone biosynthesis-related genes (Hmgcr, Hmgcs, and Fdps) in metabolic pathways, 10 steroid biosynthesis-related genes (Fdft1, Sqle, Lss, Cyp51a1, Tm7sf2, Nsdhl, Hsd17b7, Dhcr24, Sc5d, and Dhcr7), and 3 fatty acid biosynthesis-related genes (Acaca, Fasn, and Ascl) were all up-regulated in the EAD group, suggesting that the lipid metabolism pathway and steroid biosynthesis pathway play important roles in early food habit domestication in Largemouth bass. In addition, the detection results of randomly selected 15 DEGs and 15 DEPs indicated that both transcriptome and proteome results in the study were reliable. Our study provides useful information for further research on the mechanisms of food habit domestication in fish.

In the past decade, triboelectric nanogenerators (TENGs) have attracted significant attention across various fields due to their compact size, light weight, high output voltage, versatile shapes, and strong compatibility. However, substantial wear at solid–solid contact interfaces presents a major obstacle to the electrical output stability of TENGs. The objective of this study is to investigate the output performances of TENGs lubricated with TiO2-doped oleic acid. The results suggest that the triboelectrical performances of the polyimide (PI) film sliding against a steel ball under 0.1 wt% TiO2-doped oleic acid are significantly improved compared to those under dry conditions; the growth rates are 35.2%, 103.6, and 85.6%, respectively. Moreover, the coefficient of friction dropped from 0.31 to 0.066. The wear and performance enhancement mechanism are also analyzed. This study provides an effective approach to improve both the electrical performances and tribological behaviors.

Abstract Background Brachydactyly type B is an autosomal dominant disorder that is characterized by hypoplasia of the distal phalanges and nails and can be divided into brachydactyly type B1 (BDB1) and brachydactyly type B2 (BDB2). BDB1 is the most severe form of brachydactyly and is caused by truncating variants in the receptor tyrosine kinase–like orphan receptor 2 (ROR2) gene. Case presentation Here, we report a five-generation Chinese family with brachydactyly with or without syndactyly. The proband and her mother underwent digital separation in syndactyly, and the genetic analyses of the proband and her parents were provided. The novel heterozygous frameshift variant c.1320dupG, p.(Arg441Alafs*18) in the ROR2 gene was identified in the affected individuals by whole-exome sequencing and Sanger sequencing. The c.1320dupG variant in ROR2 is predicted to produce a truncated protein that lacks tyrosine kinase and serine/threonine- and proline-rich structures and remarkably alters the tertiary structures of the mutant ROR2 protein. Conclusion The c.1320dupG, p.(Arg441Alafs*18) variant in the ROR2 gene has not been reported in any databases thus far and therefore is novel. Our study extends the gene variant spectrum of brachydactyly and may provide information for the genetic counselling of family members.

Malignant melanoma (MM) is the most metastatic and aggressive form of skin cancer, and carries a high risk of death. Immune-checkpoint inhibitor therapy and molecular-targeted therapy can prolong the survival of patients with advanced MM significantly. However, the low response rate and inevitable drug resistance prevent further improvements in efficacy, which is closely related to the tumor microenvironment (TME). The TME refers to the tumor stroma, including fibroblasts, keratinocytes, immune cells, soluble molecules, and extracellular matrix (ECM). The dynamic interaction between the TME and tumor cells is very important for the growth, local invasion, and metastatic spread of tumor cells. A patient-derived organoid (PDO) model involves isolation of tumor tissue from patients with MM and culturing it in vitro in a three-dimensional pattern. Compared with traditional cultivation methods, the PDO model preserves the heterogeneity of the tissue structure of MM and demonstrates the interaction between MM cells and the TME. It can reproduce the characteristics of proliferation, migration, and invasion of MM cells, and better simulate the structural function of MM in vivo. This review explores the role of each TME component in development of the PDO model. This review will provide a reference for research on the drug screening and targeted treatment using PDOs, particularly for the immunotherapy of MM.

Controlling the component ratios of plant essential oils is challenging in their heterologous bioproduction. Here, the authors combine metabolic and enzymatic engineering strategies to achieve the production of sandalwood oil with a desirable component ratio in baker’s yeast.

Abstract Persistence of CAR-T cell function is associated with relapse rate after CAR-T therapy, while co-stimulatory agents are highly concerned with the persistence of CAR-T cells. In this study, we designed and constructed a series of BCMA-targeting second-generation CAR constructs containing CD28, 41BB, and OX40 molecules, respectively, to identify the costimulatory domains most favorable for persistence. The results of routine in vitro studies showed that OX40-CAR-T and 41BB-CAR-T had similar antitumor effects and were superior to CD28-CAR-T in terms of proliferation and cytotoxicity. Although difficult to distinguish by conventional functional assays, OX40-CAR-T cells exhibited greater proliferation and enhanced immune memory than 41BB-CAR-T cells with the repeated stimulation assay by BCMA-expressing target cells. In vivo studies further demonstrated that OX40-CAR-T cells had stronger proliferative activity than 41BB-CAR-T cells, which was highly consistent with the in vitro antitumor activity and proliferation results. Our study provides for the first time a scientific basis for designing OX40-CAR-T cell therapy to improve relapse in patients with MM after CAR-T treatment.

Although chimeric antigen receptor (CAR) T cell immunotherapy has shown promising significance in B cell malignancies, success against T cell malignancies remains unsatisfactory because of shared antigenicity between normal and malignant T cells, resulting in fratricide and hindering CAR production for clinical treatment. Here, we report a new strategy of blocking the CD7 antigen on the T cell surface with a recombinant anti-CD7 antibody to obtain a sufficient amount of CD7-targeting CAR-T cells for T cell acute lymphoblastic leukemia (T-ALL) treatment. Feasibility was evaluated systematically, revealing that blocking the CD7 antigen with an antibody effectively blocked CD7-derived fratricide, increased the expansion rate, reduced the proportion of regulatory T (Treg) cells, maintained the stem cell-like characteristics of T cells, and restored the proportion of the CD8+ T cell population. Ultimately, we obtained anti-CD7 CAR-T cells that were specifically and effectively able to kill CD7 antigen-positive target cells, obviating the need for complex T cell modifications. This approach is safer than previous methods and provides a new, simple, and feasible strategy for clinical immunotherapies targeting CD7-positive malignant tumors.

Background The largemouth bass (Micropterus salmoides), an economically important freshwater fish species widely farmed in China, is traditionally cultured using a diet of forage fish. However, given the global decline in forage fish fisheries and increasing rates of waterbody pollution and disease outbreaks during traditional culturing, there is a growing trend of replacing forage fish with formulated feed in the largemouth bass breeding industry. The specific molecular mechanisms associated with such dietary transition in this fish are, nevertheless, poorly understood. Methods To identify single nucleotide polymorphisms (SNPs) related to food habit domestication traits and growth traits in largemouth bass fry, we initially genotyped fry using eight candidate SNPs based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, with genetic parameters being determined using Popgen32 and Cervus 3.0. Subsequently, we assessed the associations between food habit domestication traits of largemouth bass fry and these SNPs using the Chi-square test or Fisher’s exact test. Furthermore, we used a general linear model to assess the relationships between the growth traits of largemouth bass fry and these SNPs. The Pearson correlation coefficient between growth traits and the SNPs was also determined using bivariate correlation analysis in IBM SPSS Statistics 22. Finally, the phenotypic variation explained (PVE) by the SNPs was calculated by regression analysis in Microsoft Excel. Results The genotyping results obtained based on PCR-RFLP analysis were consistent with those of direct sequencing. Five SNPs (SNP01, SNP02, SNP04, SNP05, and SNP06) were found to be significantly correlated with the food habit domestication traits of fry (P < 0.05); SNP01 (P = 0.0011) and SNP04 (P = 0.0055) particularly, had showed highly significant associations. With respect to growth traits, we detected significant correlations with the two SNPs (SNP01 and SNP07) (P < 0.05), with SNP01 being significantly correlated with body length, and height (P < 0.05), and SNP07 being significantly correlated with body height only (P < 0.05). Conclusions Our findings indicated that the PCR-RFLP can be used as a low-cost genotyping method to identify SNPs related to food habit domestication and growth traits in largemouth bass, and that these trait-related SNPs might provide a molecular basis for the future breeding of new varieties of largemouth bass.

Background: Functional orthoses are commonly used to treat skeletal Class II malocclusion, but the specific mechanism through which they do this has been a challenging topic in orthodontics. In the present study, we aimed to explore the effect of tensile stress on the osteogenic differentiation of condylar chondrocytes from an exosomal perspective.Methods: We cultured rat condylar chondrocytes under resting and tensile stress conditions and subsequently extracted cellular exosomes from them. We then screened miRNAs that were differentially expressed between the two exosome extracts by high-throughput sequencing and performed bioinformatics analysis and osteogenesis-related target gene prediction using the TargetScan and miRanda softwares. Exosomes cultured under resting and tensile stress conditions were co-cultured with condylar chondrocytes for 24 h to form the Control-Exo and Force-Exo exosome groups, respectively. Quantitative real time PCR(RT-qPCR) and western blotting were then used to determine the mRNA and protein expression levels of Runx2 and Sox9 in condylar chondrocytes.Results: The mRNA and protein expression levels of Runx2 and Sox9 in the Force-Exo group were significantly higher than those in the Control-Exo group (p < 0.05). The differential miRNA expression results were consistent with our sequencing results. Bioinformatics analysis and target gene prediction results showed that the main biological processes and molecular functions involved in differential miRNA expression in exosomes under tensile stress were biological processes and protein binding, respectively. Kyoto Gene and Genome Data Bank (KEGG) pathway enrichment analysis showed significant enrichment of differentially expressed miRNAs in the mTOR signaling pathway. The differentially expressed miRNAs were found to target osteogenesis-related genes.Conclusion: These results suggest that stimulation of rat condylar chondrocytes with tensile stress can alter the expression levels of certain miRNAs in their exosomes and promote their osteogenic differentiation. Exosomes under tensile stress culture conditions thus have potential applications in the treatment of Osteoarthritis (OA).

We revise the taxonomy of the genus Ptychoptera Meigen, 1803 from Zhejiang, East China. One new species from Zhejiang, P. tianmushana sp. nov., is described and illustrated. Morphologically, the new species is most similar to P. emeica Kang, Xue & Zhang, 2019 and P. formosensis Alexander, 1924, but it can be distinguished by the coloration of the abdomen and the details of the male genitalia. In addition, P. bellula Alexander, 1937 is recorded from Zhejiang for the first time. Two known species from Zhejiang, P. longwangshana Yang & Chen, 1998 and P. gutianshana Yang & Chen, 1995, are redescribed and illustrated. A key to Chinese species of Ptychoptera is provided.

Largemouth bass (Micropterus salmoides L.) is generally considered to comprise two subspecies, Florida bass (M. floridanus) and Northern Largemouth bass (M. salmoides), which have biological characteristic differences because of their geographical distribution. In this study, whole-genome re-sequencing was performed among 10 Florida and 10 Northern largemouth bass, respectively. In total, 999,793 SNPs and 227,797 InDels were finally identified, and 507,401 SNPs (50.75%) and 116,213 InDels (51.01%) were successfully mapped to annotated 18,629 genes and 14,060 genes, respectively. KEGG classification indicated that most of these genes were focused on the pathways including signal transduction, transport and catabolism, and endocrine system. Genetic diversity analysis indicated that Florida largemouth bass had higher genetic diversity than Northern largemouth bass, indicating that the germplasm quality of Northern largemouth bass had markedly reduced in China. To examine the accuracies of the identified markers, 23 SNPs and eight InDels (the insertions or deletions of more than 45 bp) were randomly selected and detected among Florida largemouth bass, Northern largemouth bass, and their F1 hybrids. The detection efficiencies of all the markers were higher than 95%; nineteen SNPs and three InDels could accurately distinguish the two subspecies and their F1 hybrids with 100% efficiencies. Moreover, the three InDel markers could clearly distinguish the two subspecies and their F1 hybrids with a PCR-based agarose gel electrophoresis. In conclusion, our study established a simple PCR-based method for the germplasm identification of largemouth bass, which will be useful in the germplasm protection, management, and hybridization breeding of largemouth bass.

Multi-functional trade-offs and synergy research on land systems are hotspots in geography and land science research, and are of great significance for achieving sustainable development of land use and the effective allocation of land resources. Recently, the development of the western region and The Belt and Road Initiative have become key topics, bringing opportunities and challenges to Xi’an. The rapid development of cities is accompanied by drastic changes in land use, and the ecological problems in the Qinling Mountains are becoming increasingly severe. This study took Xi’an as a case study and quantitatively evaluated the spatial-temporal patterns and trade-offs of land system functions such as economic development (ED), grain production (GP), ecological service (ES), etc. on the scale of 1 km × 1 km by fusing the data on land use, topography, soil, climate, and social economy. The results showed that the ED function of the land system continued to rise between 1980 and 2015, the GP function first declined and then increased; however, the ES function continued to decline. The ED, GP and ES functions respectively present a spatial pattern of high-value agglomeration, high in the north and low in the south, and high in the south and low in the north. In general, the three land system functions were trade-offs between each other. In terms of spatial pattern, ED and ES functions showed trade-offs in the south and a synergy distribution in the north; ESs and GP function trade-off zone significantly larger than the synergy zone, the trade-off between the two was significant; while the trade-off and the synergy zone for GP and ED was relatively small, the trade-off zone was the main one. The significant trade-off between GP and ES functions of the land system is a serious problem in land use in Xi’an. Under the premise of limited arable land, it is the current feasible strategy to promote the high-quality development of agriculture to increase the cultivation rate and efficiency, and to strengthen the ecological protection of arable land. In addition, the continued decline of ES functions is also worthy of attention. It is necessary to focus on increasing the greening rate of the city and strengthening the ecological management of the northern foot of the Qinling Mountains.

The assessment of populations affected by urban flooding is crucial for flood prevention and mitigation but is highly influenced by the accuracy of population datasets. The population distribution is related to buildings during the urban floods, so assessing the population at the building scale is more rational for the urban floods, which is possible due to the abundance of multi-source data and advances in GIS technology. Therefore, this study assesses the populations affected by urban floods through population mapping at the building scale using highly correlated point of interest (POI) data. The population distribution is first mapped by downscaling the grid-based WorldPop population data to the building scale. Then, the population affected by urban floods is estimated by superimposing the population data sets onto flood areas, with flooding simulated by the LISFLOOD-FP hydrodynamic model. Finally, the proposed method is applied to Lishui City in southeast China. The results show that the population affected by urban floods is significantly reduced for different rainstorm scenarios when using the building-scale population instead of WorldPop. In certain areas, populations not captured by WorldPop can be identified using the building-scale population. This study provides a new method for estimating populations affected by urban flooding.

With the continuous development of the convolutional neural network (CNN) concept and other deep learning technologies, target recognition in Synthetic Aperture Radar (SAR) images has entered a new stage. At present, shallow CNNs with simple structure are mostly applied in SAR image target recognition, even though their feature extraction ability is limited to a large extent. What’s more, research on improving SAR image target recognition efficiency and imbalanced data processing is relatively scarce. Thus, a lightweight CNN model for target recognition in SAR image is designed in this paper. First, based on visual attention mechanism, the channel attention by-pass and spatial attention by-pass are introduced to the network to enhance the feature extraction ability. Then, the depthwise separable convolution is used to replace the standard convolution to reduce the computation cost and heighten the recognition efficiency. Finally, a new weighted distance measure loss function is introduced to weaken the adverse effect of data imbalance on the recognition accuracy of minority class. A series of recognition experiments based on two open data sets of MSTAR and OpenSARShip are implemented. Experimental results show that compared with four advanced networks recently proposed, our network can greatly diminish the model size and iteration time while guaranteeing the recognition accuracy, and it can effectively alleviate the adverse effects of data imbalance on recognition results.