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With the implementation of “three accesses and two platforms” (three accesses refer to the access to Internet for each school and college, access to quality educational resources for each class, access to online learning space for each student; two platforms refer to the online platform for educational resources and the online platform for educational management), the online teaching space with more abundant subject knowledge material has become a new teaching field of curriculum reform. Its effectiveness depends on the multiple effects of value pursuit, identity, teaching system, teaching culture, teaching personality and learning style, with students’ identity as the subjective initiative factor. In view of the fact that the purpose of education is the premise and foundation of students’ identity, this paper applies the methods of sociology and pedagogy to explain the influence of the value orientation of students’ identity and the socialization process of learners’ development so as to illustrate the dynamic developing strategies of the construction of self-management learners’ identity in online teaching space on the basis of the building, developing, formatting and maintenance of emotional education of teaching.

A superabsorbent resin (SAR) with good degradability was prepared with acrylic acid (AA), acrylamide (AM), 2-acrylamido-2-methylpropane sulfonic acid (AMPS), organic montmorillonite (OMMT) and titanium dioxide (TiO2) through inverse suspension polymerization, using potassium persulfate (KPS) as initiator and N,N-methylenebisacrylamide (MBA) as crosslinker. The structure and micrographs of the SAR were fully characterized with Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD). Meanwhile, the effects of major factors such as initiator, crosslinker, OMMT and TiO2 contents and the oil–water ratio on liquid absorbency were investigated, which reached 769.3 g/g in distilled water and 91.1 g/g in 0.9wt% NaCl solution, respectively. The equilibrium degradation performances were evaluated under the optimum conditions by the mass loss of the SAR in the soil and under the ultraviolet light, which reached 32.3wt% after 120 days and 83.9wt% after 40 h, respectively. The introduction of OMMT promoted the photodegradability obviously. The maximum photodgradation of PAA/TiO2 SAR reached 74.3wt% after 60 h, which was lower than the PAA/OMMT/TiO2 SAR. The degradable SAR has an encouraging potential for agricultural and forestry applications.

The rational design and construction of heterojunction structure is an effective strategy to improve the photocatalytic performance. Herein, a series of BiOBr nanosheets-immobilized TiO2/Ti3C2Tx MXene hybrid materials with heterojunction structure were synthesized by a facial one-step hydrothermal method. The ternary composites show outstanding performance as photocatalysts for the degradation of rhodamine B due to the optimized synergetic effects of BiOBr, TiO2 and Ti3C2Tx. The improved photocatalytic performance is remarkably attributed to the construction of a heterojunction between TiO2 and BiOBr due to their well-matching of energy band position, which can enhance the absorption for visible light and promote the transfer of photo-generated charge carriers. Moreover, Ti3C2Tx acts as an electron trap to further accelerate the separation of photo-generated electrons and holes.

In this paper, we present an open python procedure with Jupyter notebook, for data extraction and vectorization of geophysical exploration profile. Constrained by observation routes and traffic conditions, geophysical exploration profiles tend to bend curved roads for easy observation, however, it must be projected onto a straight line when data processing and analyzing. After projection, we don't know the true position of the obtained crustal structure. Nonetheless, when the results used as an initial constraint condition for other geophysical inversion, such as gravity inversion, we need to know the true position of the data rather than the distance to the starting point. We solved this problem by profile vectorization and reprojection. The method can be used for extraction data of various geophysical exploration profiles, such as seismic reflection profiles, gravity profiles. Keywords: Data extraction, Vectorization, Profile, Geophysical exploration, Python

Abnormal lipid metabolism plays crucial roles in the development of cancer. Spindlin 1 (SPIN1) involving the process of spindle organization and chromosomal stability serves as an important player in the carcinogenesis. In this study, we try to identify the new function of SPIN1 in lipid metabolism of liver cancer. Tissue microarray showed that 75% (60/80) of hepatocellular carcinoma (HCC) tissues were positive for SPIN1, which was highly expressed in clinical HCC samples and positively associated with malignancy of HCC. Strikingly, SPIN1 could modulate abnormal lipid metabolism by increasing intracellular triglycerides, cholesterols, and lipid droplets in hepatoma cells, which could remarkably enhance the proliferation of hepatoma cells. Mechanistically, SPIN1 up-regulated FASN in hepatoma cells. SPIN1 co-activated transcriptional factor SREBP1c in the promoter of FASN through interaction with SREBP1c. Moreover, SPIN1 promoted the growth of liver cancer in vitro and in vivo and the levels of intracellular triglycerides, cholesterols and lipid droplets were increased in the tumor tissues from mice. In conclusion, SPIN1 modulates abnormal lipid metabolism and enhances growth of liver cancer through SREBP1c-triggered FASN signaling. Therapeutically, SPIN1 may serve as a novel target for HCC.

CO2-oxidative dehydrogenation of propane (CO2-ODHP) provides a promising route for propylene production. Sufficient propane conversion and propylene selectivity remain a great challenge due to the difficulty in activating inert propane and CO2 simultaneously. Herein, a Sn doped CeO2 supported Pt (Pt/Sn-CeO2) catalyst in CO2-ODHP reaction is reported. Sn doping appears to kill two birds with one stone for propane and CO2 activation. On the one side, it increases the electron density of Pt species via PtSn alloy formation, promoting propane adsorption and C−H bond cleavage. On the other side, it enhances oxygen vacancy concentrations of CeO2 support, facilitating CO2 dissociation. A higher propylene selectivity (63.9 % vs 22.3 %) was obtained on 0.1 wt % Pt/1.0 wt % Sn-CeO2 than that on 0.1 wt % Pt/CeO2 with a comparable propane conversion (15.1 % vs 14.3 %) at 550 °C after 240 min on stream. This work provides a reference for designing efficient catalysts.

The aim of this research is to investigate the sorptivity and capillary coefficient in unsaturated concrete using the capillary water absorption experiment. The effects of water-binder rati...

Objective: N6-methyladenosine (m6A) modification may modulate various biological processes. Nonetheless, clinical implications of m6A modification in pancreatic cancer are undefined. Herein, this study comprehensively characterized the m6A modification patterns in pancreatic cancer based on m6A regulators.Methods: Genetic mutation and expression pattern of 21 m6A regulators and their correlations were assessed in pancreatic cancer from TCGA dataset. m6A modification patterns were clustered using unsupervised clustering analysis in TCGA and ICGC datasets. Differences in survival, biological functions and immune cell infiltrations were assessed between modification patterns. A m6A scoring system was developed by principal component analysis. Genetic mutations and TIDE scores were compared between high and low m6A score groups.Results: ZC3H13 (11%), RBM15B (9%), YTHDF1 (8%), and YTHDC1 (6%) frequently occurred mutations among m6A regulators. Also, most of regulators were distinctly dysregulated in pancreatic cancer. There were tight crosslinks between regulators. Two m6A modification patterns were constructed, with distinct prognoses, immune cell infiltration and biological functions. Furthermore, we quantified m6A score in each sample. High m6A scores indicated undesirable clinical outcomes. There were more frequent mutations in high m6A score samples. Lower TIDE score was found in high m6A score group, with AUC = 0.61, indicating that m6A scores might be used for predicting the response to immunotherapy.Conclusion: Collectively, these data demonstrated that m6A modification participates pancreatic cancer progress and ornaments immune microenvironment, providing an insight into pancreatic cancer pathogenesis and facilitating precision medicine development.

The overall goal of this research was to examine the mechanical, water vapor barrier properties and opacity of films prepared using legume protein concentrates (faba bean, pea, lupin, lentil, and soy) as a function of glycerol concentration (50, 75, or 100% [wt/wt]-relative to the protein weight). Overall, tensile strength (TS) decreased with increasing glycerol concentration, whereas tensile elongation (TE) and water vapor permeability (WVP) increased with increasing glycerol concentration. Film opacity was independent of glycerol concentration. The effect of protein-type varied considerably depending on the functional property of the film being measured; TS was greatest with faba bean and lowest with lupin, whereas TE was highest for pea, and lowest for soy. Lentil protein films had considerably higher WVP, at the 100% glycerol concentration, as compared to the other protein concentrates. Findings from this study indicate that legume protein concentrates are capable of forming biodegradable, edible films. Overall, pea protein concentrate films showed the most promise for application in terms of strength, elongation, and moisture barrier properties.

The b-value is closely related to the crustal stress. By studying the b-value trends of regions before large earthquakes, we can obtain the evolution of crustal stress, which is generally considered useful earthquake precursor information. A quake is closely related to fault tectonic movement. In the past, the traditional b-value calculation method did not consider the spatial distribution of the fault. Here, we propose a new algorithm based on the fault’s buffer area, combining the spatial distribution and trend of the fault. This algorithm can accurately calculate the b-value changes for a specific fault using the fault as the basic independent unit. This method provides a useful reference for earthquake hazard judgment. Based on this algorithm, we use a total of 428,963 earthquakes from 1980 to 2013 after removal of the aftershock sequences and combine these quakes with the active fault distribution data to study the b-value changes before major earthquakes in western Sichuan. The results show that the b-values of the Fubianhe fault, Longmenshan main fault, and Shuangshi–Pengguan fault in western Sichuan decreased for at least 2 years before the earthquakes. The Longmenshan main fault area began to decrease slowly 5 years before the Wenchuan Ms8.0 earthquake and sharply decreased in the year before the quake, which reflects a significant change in crustal stress during this time. This conclusion and the method are available for long-term earthquake hazard assessment as a reference.

The Barkam–Jiuzhaigou–Wuqi gravity profile extends across the Jiuzhaigou Ms7.0 earthquake (in 2017) zone and passes through several historical big earthquakes’ zones. We have obtained Bouguer gravity anomalies along the profile composed of 365 gravity observation stations with Global Positioning System (GPS) coordinates, analyzed the observed data and inverted subsurface density structure. The results show that the Moho depth has a big lateral variation from southwest to northeast, which shallows from 57 km to 43 km with maximum variation up to 14 km within 800 km. The most acute depth change of the Moho is in the boundary region between the Bayan Har block and West Qinling–Qilian block. According to our analysis, it is related to the eastward movement of the Bayan Har block. There are three main pieces of evidence that support it: (1) Density is higher in the east of the Bayan Har block and smaller in the west, which is the same as seismic activity, (2) Two thin low-density layers exist in the upper and middle crust of the Bayan Har block, which may promote inter-layer slip and the Jiuzhaigou Ms7.0 earthquake occurred in the boundary area of the two low-density layers, where the crustal density and Moho surface fluctuate sharply, (3) the GPS velocity field in the southwestern part gravity profile is significantly larger than that of the northeastern part, which is consistent with the density structure. Our studies also suggest that the large undulation of the Moho prevents the movement of the Bayan Har block, and strain is prone to accumulate here. The dynamic background analysis of the crust in this area indicates that the Moho surface uplifts in the West Qinling–Qilian block, which decelerates the eastern migration of material on the Qinghai–Tibet Plateau, and leads to the weak tectonic activity of the north part of the Bayan Har block.

Previous studies show that the calculated loading effects from global ocean tide models do not match actual measurements of gravity attraction and loading effects in Southeast Asia. In this paper, taking advantage of a unique network of gravity tidal stations all over the Chinese mainland, we compare the observed and modeled tidal loading effects on the basis of the most recent global ocean tide models. The results show that the average efficiencies of the ocean tidal loading correction for O1, K1, M2 are 77%, 73% and 59%, respectively. The loading correction efficiencies using recent ocean tidal models are better than the 40 years old Schwiderskis model at coastal stations, but relative worse at stations far from ocean.

Chronic infection of HBV is a serious global health issue. However, the HBV covalently closed circular DNA (cccDNA) cannot be completely cleaned in liver. Here, we engineer a novel polypeptide, termed D-TTK001, targeting HBV X protein (HBx) against HBV cccDNA. D-TTK001 is a D-type polypeptide, which has a high binding affinity to HBx. Interestingly, it is able to against HBV efficiently in primary human hepatocytes (PHHs) from three donors. D-TTK001 displays high activities against HBV, remarkably reducing the levels of cccDNA in the HBV-infected human liver-chimeric mice. Mechanically, D-TTK001 depresses cccDNA transcription by regulating the epigenetic modification of cccDNA minichromosome and disturbing the interaction of HBx with DDB1 in the cells. D-TTK001 decreases the levels of cccDNA by down-regulating MSL2, leading to the increase of APOBEC3B in liver. When combined with entecavir or interferon-α, D-TTK001 shows additive antiviral effects. Clinically, D-TTK001 is a strong candidate to clear cccDNA against HBV.

Background Hepatitis B virus covalently closed circular DNA (HBV cccDNA) is assembled by histones and non-histones into a chromatin-like cccDNA minichromosome in the nucleus. The cellular histone acetyltransferase GCN5, displaying succinyltransferase activity, is recruited onto cccDNA to modulate HBV transcription in cells. Clinically, IFN-α is able to repress cccDNA. However, the underlying mechanism of IFN-α in the depression of cccDNA mediated by GCN5 is poorly understood. Here, we explored the effect of IFN-α on GCN5-mediated succinylation in the epigenetic regulation of HBV cccDNA minichromosome. Results Succinylation modification of the cccDNA minichromosome has been observed in HBV-infected human liver-chimeric mice and HBV-expressing cell lines. Moreover, histone H3K79 succinylation by GCN5 was identified in the system. Interestingly, the mutant of histone H3K79 efficiently blocked the replication of HBV, and interference with GCN5 resulted in decreased levels of HBV DNA, HBsAg, and HBeAg in the supernatant from de novo HBV-infected HepaRG cells. Consistently, the levels of histone H3K79 succinylation were significantly elevated in the livers of HBV-infected human liver-chimeric mice. The knockdown or overexpression of GCN5 or the mutant of GCN5 could affect the binding of GCN5 to cccDNA or H3K79 succinylation, leading to a change in cccDNA transcription activity. In addition, Southern blot analysis validated that siGCN5 decreased the levels of cccDNA in the cells, suggesting that GCN5-mediated succinylation of histone H3K79 contributes to the epigenetic regulation of cccDNA minichromosome. Strikingly, IFN-α effectively depressed histone H3K79 succinylation in HBV cccDNA minichromosome in de novo HepG2-NTCP and HBV-infected HepaRG cells. Conclusions IFN-α epigenetically regulates the HBV cccDNA minichromosome by modulating GCN5-mediated succinylation of histone H3K79 to clear HBV cccDNA. Our findings provide new insights into the mechanism by which IFN-α modulate the epigenetic regulation of HBV cccDNA minichromosome.

Background Clinical findings reported that some cases of Juvenile Idiopathic Arthritis(JIA) gradually suffer from hyperuricemia(HUA) during the course of follow-up, followed by the detection of high urate crystal, gout, renal impairment, and other manifestations. And thoes patients would influence the prognosis. Methods This was a retrospective study of 60 patients diagnosed with JIA between October 2016 and March 2019 and followed up for > 1 year. Single-factor analyses of the clinical data, laboratory data, and the special drug used for JIA complicated with hyperuricemia (Group A, n = 18) and JIA without hyperuricemia (Group B, n = 42) were conducted. Results Comparison between Groups A and B revealed differences in sex; disease course; high disease activity; diastolic pressure; the levels of serum albumin (ALB), alanine aminotransferase (ALT), aspartate transaminase (AST), triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), glucose (GLU), and urea nitrogen (BUN); NSAIDS application, systemic glucocorticoid application, MTX application, and tumor necrosis factor-alpha (TNF-α) inhibitors application were not statistically significant (P > 0.05). However, the differences in age, active sacroiliitis, body mass index (BMI), systolic pressure, serum creatinine (Scr) level, and salicylazosulfapyridine (SASP) application showed a statistical significance (P

We applied a 2-dimensional, non-spectral technique to investigate the spatial variations of the lithospheric effective elastic thickness (Te) in the eastern Bayan Har block and its adjacent areas. Te values were determined by comparison of observed and flexural modelled Bouguer gravity anomalies. The results suggested that Te vary significantly from eastern Tibet to the Sichuan basin. The eastern Bayan Har block and northern Sichuan-Yunan block had low Te (0

In this paper, the problem of integrating VR technology to introduce user's behavior and feelings into green building design is studied. According to the practical experience and comparative analysis, it is proposed that Revit and Mars are the main software tools of BIM + VR technology; the application of BIM + VR technology is analyzed from four aspects of site design, general layout, architectural form design and interior space design respectively. This paper discusses how to open VR scene experience to users from the aspects of scene perfection, with experience mode selection, feedback collection and arrangement, and how to modify the scheme design. Finally, combined with VR technology, this paper makes a preliminary exploration on introducing the user's behavior and psychological feelings into the process of green building design, and applies them in the process of building design of a university campus, which verifies the effectiveness of our scheme.

Lysine succinylation (Ksucc) is an evolutionarily conserved and widespread post-translational modification. Histone acetyltransferase 1 (HAT1) is a type B histone acetyltransferase, regulating the acetylation of both histone and non-histone proteins. However, the role of HAT1 in succinylation modulation remains unclear. Here, we employ a quantitative proteomics approach to study succinylation in HepG2 cancer cells and find that HAT1 modulates lysine succinylation on various proteins including histones and non-histones. HAT1 succinylates histone H3 on K122, contributing to epigenetic regulation and gene expression in cancer cells. Moreover, HAT1 catalyzes the succinylation of PGAM1 on K99, resulting in its increased enzymatic activity and the stimulation of glycolytic flux in cancer cells. Clinically, HAT1 is significantly elevated in liver cancer, pancreatic cancer, and cholangiocarcinoma tissues. Functionally, HAT1 succinyltransferase activity and the succinylation of PGAM1 by HAT1 play critical roles in promoting tumor progression in vitro and in vivo. Thus, we conclude that HAT1 is a succinyltransferase for histones and non-histones in tumorigenesis.

Hepatitis B virus (HBV) is a major risk factor for liver diseases, in which HBV covalently closed circular DNA (cccDNA), as the genomic form that templates viral transcription, plays crucial roles in sustaining viral persistence. Clinically, the excessive ethanol intake accelerates the progression of liver diseases with HBV infection. Here, we supposed that ethanol might trigger HBV cccDNA in the liver. Interestingly, we observed that the ethanol remarkably elevated the levels of HBeAg, HBsAg, HBV DNA and cccDNA in HBV-expressing hepatoma cells. Mechanically, the ethanol increased the levels of HBx and MSL2 in vivo and in HBV-expressing HepG2 cells, but not in HBV-free HepG2 cells. Moreover, the down-regulation of MSL2 by small interference RNA could block the ethanol-promoted HBV cccDNA in HepG2.2.15 cells. As a commonly administered treatment for HBV, the effect of IFNα on ethanol-triggered HBV cccDNA remains poorly understood. Strikingly, we showed that the treatment with IFN-α2b inhibited the ethanol-promoted cccDNA through depressing MSL2 in the cells. Thus, we conclude that IFN-α2b inhibits the ethanol-enriched HBV cccDNA through blocking a positive feedback loop of HBx/MSL2/cccDNA/HBV/HBx. Our finding provides new insights into the mechanism by which IFN-α2b inhibits ethanol-enhanced HBV cccDNA. Therapeutically, IFNα may contribute to the cccDNA induced by ethanol in liver.

Diffusion and convection are the main factors affecting chloride ion transport in unsaturated cementitious materials. In this study, capillary salt absorption experiment and steady-state migration experiment were used to investigate the chloride ion migration in unsaturated concrete, and a modified chloride ion diffusion-convection model was established for concrete considering curing age, initial water saturation and the combined effect of chloride ion. The results show that the chloride ion migration in unsaturated concrete can be divided into six stages: safety stage, initial stage, convection stage, deceleration stage, diffusion stage and stablilization stage, the chloride ion binding coefficient is approximately linear with the initial saturation, and the concrete with fly ash content of 40% or sand content of 60% has the best blocking effect on chloride ion migration.

In this paper, we calculated Bouguer gravity anomalies and Airy-Heiskanen isostatic anomalies in the New Britain Trench and surrounding areas of Papua New Guinea. The calculations are based on a topographic model and a gravity anomaly model from the Scripps Institute of Oceanography. Characteristics of the isostatic anomaly and the earthquake dynamic environment of this region are analyzed, and the results show that there are obvious differences in the isostatic state between each block in the region. Tectonic movements are very intense in regions with high positive or negative isostatic gravity anomalies, and a number of sub-plates in this area are driven by external tectonic action, such as plate subduction and thrusting, of the Pacific, Indo-Australian and Eurasian plates. From the distribution of the isostatic gravity anomaly, the tectonic action of anti-isostatic movement obviously is the main source of power. Based on isostatic gravity and spatial distribution of earthquakes in the region, it is obvious that further contraction of the Indo-Australian Plate will result in the southwestern part of the Solomon Plate becoming part of the Owen Stanley thrust belt, and the northern part will enter the lower part of the Bismarck Plate. The eastern part of the Solomon Plate will enter the front of the Pacific Plate, resulting in northward and eastward migration of significant earthquakes along the Solomon Plate. Keywords: Papua New Guinea, New Britain Trench, Earthquake, Bouguer gravity anomaly, Isostatic gravity anomaly

Chemokine monocyte chemoattractant protein-1 (MCP-1) has been proved as a potential urinary biomarker in nephropathies. The aim of this study was to investigate the urinary monocyte chemoattractant protein-1 (MCP-1) levels and clinical significance in Henoch-Schonlein purpura (HSP) children with and without nephritis and determine the association of MCP-1 with proteinuria. Methods: A total of 261 HSP children—with or without nephritis—and 84 healthy control children were enrolled in this study. Of these, 126 HSP nephritis (HSPN) children were subdivided into three groups according to total urine protein in 24 h (TUP): Group A, mild proteinuria group with TUP 0.05). The levels of urinary MCP-1 increased in parallel to the enhancement of total urine protein in 24 h in HSPN patients. There were statistically significant differences among these three groups of HSPN children (p