1. Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications
- Author
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Linlin Zhuang, Jiansen Gong, Ping Zhang, Di Zhang, Ying Zhao, Jianbo Yang, Guofang Liu, Yu Zhang, and Qiuping Shen
- Subjects
Salmonella ,Loop-mediated isothermal amplification ,Detection methods ,Target genes ,Food safety applications ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
Abstract Salmonella, the prevailing zoonotic pathogen within the Enterobacteriaceae family, holds the foremost position in global bacterial poisoning incidents, thereby signifying its paramount importance in public health. Consequently, the imperative for expeditious and uncomplicated detection techniques for Salmonella in food is underscored. After more than two decades of development, loop-mediated isothermal amplification (LAMP) has emerged as a potent adjunct to the polymerase chain reaction, demonstrating significant advantages in the realm of isothermal amplification. Its growing prominence is evident in the increasing number of reports on its application in the rapid detection of Salmonella. This paper provides a systematic exposition of the technical principles and characteristics of LAMP, along with an overview of the research progress made in the rapid detection of Salmonella using LAMP and its derivatives. Additionally, the target genes reported in various levels, including Salmonella genus, species, serogroup, and serotype, are summarized, aiming to offer a valuable reference for the advancement of LAMP application in Salmonella detection. Finally, we look forward to the development direction of LAMP and expect more competitive methods to provide strong support for food safety applications.
- Published
- 2024
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