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Recently, the Mg–Ag binary alloy has gained much attention as an antibacterial biomaterial. However, the relationships between the extrusion parameters, microstructure, and mechanical properties of Mg–Ag alloys haven't been clearly established. Therefore, in this paper, the effects of silver (Ag) content (0, 3, 6 wt%) and extrusion (as cast, extrusion ratio (ER) 7.1 and 72.2) on the microstructure and mechanical properties of Mg–Ag alloys are systematically investigated. The results indicate that for the Mg–Ag alloys with the same extrusion ratio, as the Ag increased from 0 to 3 wt%, Mg4Ag and Mg54Ag17 precipitated, leading to significant precipitation strengthening and an increase in the yield strength by over 137%. As the Ag increased to 6 wt%, the yield strength increased by only ∼20%, attributable to Ag promoting dynamic recrystallization, resulting in less grain refinement, a slower amount increase and a smaller size reduction of the precipitate. For the Mg–Ag alloys with the same Ag content, as the extrusion ratio increased to 7.1, the yield strength increased significantly by over 37%, since the grain boundary strengthening and precipitation strengthening were significantly enhanced as the grains were refined, a large number of precipitates appeared, and their sizes significantly reduced. When the extrusion ratio increased to 72.2, the yield strength increased by only ∼10%, due to the weakening of both grain boundary strengthening and precipitation strengthening as the recrystallization further improved, leading to an increase in grain sizes, the redissolution of precipitates, and a lesser reduction in their sizes.

Molten salt from the service environment leaches Y2O3 from yttria-stabilized zirconia (YSZ), threatening the security of thermal barrier coatings (TBCs). In this study, we compared the molten salt corrosion resistances of YSZ coatings prepared via plasma-spraying physical vapor deposition (PS-PVD) before and after laser remelting. At 1000 °C, the molten salt reacted with YSZ particulates in the PS-PVD YSZ coating and infiltrated the gaps between quasi-columnar crystals, resulting in phase transition of the coating and column breakage. A dense glazed layer was formed on the YSZ coating surface after remelting by the low-energy-density laser, which prohibited the penetration of molten salt and improved the hot corrosion resistance of the YSZ coating. By contrast, the failure of the high-energy-density laser-treated YSZ coating was accelerated due to the significant interfacial stress from the thick glazed layer and volume expansion caused by the corrosion products. Our investigation revealed the structural evolution of the PS-PVD YSZ coating under hot corrosion conditions, emphasized the role of laser remelting, and could serve as a guideline for TBCs modification.

In recent years, there has been widespread attention on hypergraphs as a research hotspot in network science.The unique structure of hypergraphs, which differs from traditional graphs, is characterized by hyperedges that can connect multiple nodes simultaneously, resulting in more complex and higher-order relationships.Effectively identifying important nodes and hyperedges in such network structures poses a key challenge.Eigenvector centrality, a common metric, has limitations in its application due to its locality when dealing with hub nodes with extremely high degree values in the network.To address this issue, the hypergraphs were transformed into their corresponding line graphs, and non-backtracking matrix centrality was employed as a method to measure the importance of hyperedges.This approach demonstrated better uniformity and differentiation in assessing the importance of hyperedges.Furthermore, the application of both eigenvector centrality and non-backtracking matrix centrality in assessing the importance of nodes in hypergraphs was explored.Comparative analysis revealed that non-backtracking matrix centrality effectively distinguished the importance of nodes.This research encompassed theoretical analysis, model construction, and empirical studies on real-world data.To validate the proposed method and conclusion, six real-world hypergraphs were selected as experimental subjects.The application of these methods to these hypergraphs confirmed the effectiveness of non-backtracking matrix centrality in identifying important nodes and hyperedges.The findings of this research offer a fresh perspective and approach for identifying key elements in hypergraphs, holding significant theoretical and practical implications for understanding and analyzing complex network systems.

Unmanned aerial vehicles (UAVs) can be utilized as airborne base stations to deliver wireless communication and federated learning (FL) training services for ground vehicles. However, most existing studies assume that vehicles (clients) and UAVs (model owners) offer services voluntarily. In reality, participants (FL clients and model owners) are selfish and will not engage in training without compensation. Meanwhile, due to the heterogeneity of participants and the presence of free-riders and Byzantine behaviors, the quality of vehicles’ model updates can vary significantly. To incentivize participants to engage in model training and ensure reliable outcomes, this paper designs a reliable incentive mechanism (FedBeam) based on game theory. Specifically, we model the cooperation problem between model owners and clients as a two-layer Stackelberg game and prove the existence and uniqueness of the Stackelberg equilibrium (SE). For the cooperation among model owners, we formulate the problem as a coalition game and based on this, analyze and design a coalition formation algorithm to derive the Pareto optimal social utility. Additionally, to achieve reliable FL model updates, we design a weighted-beta (Wbeta) reputation update mechanism to incentivize FL clients to provide high-quality model updates. The experimental results show that compared to the baselines, the proposed incentive mechanism improves social welfare by 17.6% and test accuracy by 5.5% on simulated and real datasets, respectively.

In this work, the corrosion behavior of pure Mg, Mg3Ag, Mg6Ag, and MgZnYNd alloys in different fixatives (ethyl alcohol (EA), 85 % ethyl alcohol (85 % EA), 10 % neutral buffered formalin (10 % NBF), 4 % glutaric dialdehyde (4 % GD), and 4 % paraformaldehyde (4 % PFA)) was investigated to provide a valuable reference for the selection of fixatives during the histological evaluation of Mg implants. Through the hydrogen evolution test, pH test, and corrosion morphology and product characterization, it was found that corrosion proceeded slowest in the EA and 85 % EA groups, slightly faster in 4 % GD, faster in 10 % NBF, and fastest in 4 % PFA. After corrosion, the EA group surface remained unchanged, while the 85%EA group surface developed minor cracks and warping. The 4%GD fixative formed a dense needle-like protective layer on the Mg substrate. The 10%NBF group initially grew a uniform layer, but later developed irregular pits due to accelerated corrosion. In contrast, the 4%PFA solution caused more severe corrosion attributed to chloride ions. The main corrosion products in the EA and 85%EA groups were MgO and Mg(OH)2, while the other fixatives containing diverse ions also yielded phosphates like Mg3(PO4)2 and MgHPO4. In 4 % PFA, AgCl formed on the surface of Mg6Ag alloy after corrosion. Therefore, to minimize Mg alloy corrosion without compromising staining quality, EA or 85 % EA is recommended, while 4 % PFA is not recommended due to its significant impact.

Abstract Surface modification is an important approach to improve osseointegration of the endosseous implants, however it is still desirable to develop a facile yet efficient coating strategy. Herein, a metal–phenolic network (MPN) is proposed as a multifunctional nanocoating on titanium (Ti) implants for enhanced osseointegration through early immunomodulation. With tannic acid (TA) and Sr2+ self‐assembled on Ti substrates, the MPN coatings provided a bioactive interface, which can facilitate the initial adhesion and recruitment of bone marrow mesenchymal stem cells (BMSCs) and polarize macrophage toward M2 phenotype. Furthermore, the TA‐Sr coatings accelerated the osteogenic differentiation of BMSCs. In vivo evaluations further confirmed the enhanced osseointegration of TA‐Sr modified implants via generating a favorable osteoimmune microenvironment. In general, these results suggest that TA‐Sr MPN nanocoating is a promising strategy for achieving better and faster osseointegration of bone implants, which can be easily utilized in future clinical applications.

Bone fractures and critical-size bone defects are significant public health issues, and clinical treatment outcomes are closely related to the intrinsic properties of the utilized implant materials. Zinc (Zn)-based biodegradable metals (BMs) have emerged as promising bioactive materials because of their exceptional biocompatibility, appropriate mechanical properties, and controllable biodegradation. This review summarizes the state of the art in terms of Zn-based metals for bone repair and regeneration, focusing on bridging the gap between biological mechanism and required bioactivity. The molecular mechanism underlying the release of Zn ions from Zn-based BMs in the improvement of bone repair and regeneration is elucidated. By integrating clinical considerations and the specific bioactivity required for implant materials, this review summarizes the current research status of Zn-based internal fixation materials for promoting fracture healing, Zn-based scaffolds for regenerating critical-size bone defects, and Zn-based barrier membranes for reconstituting alveolar bone defects. Considering the significant progress made in the research on Zn-based BMs for potential clinical applications, the challenges and promising research directions are proposed and discussed.

Invisible orthodontic treatment is an effective form of malocclusion treatment favored in recent years. The magnitude of its orthodontic force has a crucial impact on the outcome of the treatment and has gained a high level of clinical interest. However, there are very few explorations of in vivo measurements of orthodontic force, and existing studies are limited to a large number of couplings, which are inconvenient for clinical use. In this work, we developed a wireless flexible measurement system that allows quantitative measurement of the orthodontic force of an invisible aligner on a dental model. The system is wireless, tiny, flexible, fast responding, and has a range suitable for the range of orthodontic forces. We show the difference in the orthodontic force applied to different tooth positions and the difference in the orthodontic force applied to different positions of the same tooth. In addition, the system can evaluate the mechanical differences between aligners of different brands and materials as well as the deviation of fabrication results. This system provides a test tool and evaluation method for future real-time assessment of clinical orthodontic forces.

This study investigated the corrosion resistance and mechanical properties of Mg-2Zn-0.46Y-0.5Nd (wt.%) alloy plates and screws with fluorinated coatings and atomic layer deposition (ALD)-derived zirconia (ZrO2) coatings in vitro under physiological stress conditions. Synthetic polyurethane hemimandible replicas were split and fixed as the following three groups of magnesium alloy plates and screws: no additional surface coating treatment (Group A), with fluorinated coatings (Group B), and with duplex fluorinated and ALD-derived 100 nm ZrO2 coatings (Group C). A circulating stress of 1–10 N was applied to the distal bone segment, and a 4-week simulated body fluid immersion test was employed to study the remaining material volume and the mechanical properties of the different groups. Compared with Group A and Group B, the degradation rate of magnesium alloy plates and screws’ head regions was significantly slowed down under the protection of duplex MgF2/ZrO2 coatings (p < 0.01). There was no significant difference in the degradation rate of the screw shaft region between groups (p = 0.077). In contrast to fluoride coatings, duplex MgF2/ZrO2 coatings maintained the mechanical strength of magnesium alloy plates and screws after a 14 day in vitro SBF immersion test. We conclude that duplex MgF2/ZrO2 coatings exhibited a certain protective effect on the Mg alloy plates and screws under physiological stress conditions.

Recently, magnesium (Mg) alloys have attracted extensive attention as biodegradable implant materials. However, cyclic loading and the corrosive environment of the body are significant challenges for the practical use of alloys, and there are few studies on this topic. In this study, we conducted a four-point bending fatigue test for 86,400 cycles (12 h) in simulated body fluid (SBF), plasma, and whole blood with an AZ series alloy Mg-9Al-0.5Zn-0.27Mn-0.12Ag, to examine the effects of inorganic ions, organic particles, blood cells, and cyclic loading on Mg alloy corrosion. The Mg2+ concentration and solution pH were measured before and after experimentation, and the sample surfaces were characterized by 3D digital microscopy, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy (XPS).Our results showed that in the non-loading condition, a porous and weak inorganic product layer (mainly Mg/Ca phosphate and carbonate) formed on the surface of the Mg alloy sample immersed in SBF, which hardly had a protective effect on Mg alloy corrosion. For the samples immersed in plasma, the organic particles promoted the formation of an organic and more compact product layer, which protected the Mg alloy from severe corrosion. For the sample immersed in whole blood, the blood cells affected organic particle deposition on the product layer and thus interfered with the formation of an organic compact product layer, which slightly accelerated the corrosion process. Furthermore, cyclic loading damaged the layer integrity and significantly increased the corrosion rates of all the studied materials compared to the samples not subjected to cyclic loading. Nonetheless, under cyclic loading, blood cells adsorbed on the Mg alloy surfaces, and formed films, which protected the Mg alloy substrate and delayed Mg alloy corrosion.

Federated learning (FL) is a novel distributed learning framework in which clients with models can collaboratively train a high-quality global model while maintaining the privacy of their local data. The heterogeneity of datasets makes the global aggregation and local training requirements of each client in the cross-silo scenario inconsistent. Although many efforts are dedicated to improving the efficiency of cross-silo FL, the task assignment design of cross-silo FL remains an open question. To optimize the efficiency of energy consumption and training time of cross-silo FL, we develop a novel Energy-efficient and Time-sensitive Task Assignment (ETTA) mechanism. Specifically, we model clients’ requirements for energy efficiency and training time in cross-silo FL as an optimization problem, and design a utility transfer function to maintain the honesty of clients and the stability of ETTA. In terms of energy efficiency maximization, we analyze the conditions of optimal solutions based on the equilibrium of marginal cost and income, and design an approximate algorithm for cross-silo FL scenarios that require lightweight mechanisms. In terms of training time minimization, we model this objective as a min–max problem and give its analytical solution with Pareto optimality. To ensure that the task assignment achieves incentive compatibility, we further design a utility transfer selection function to incentivize honest collaboration among selfish clients. Finally, we conduct extensive experiments on the performance of ETTA to demonstrate that it outperforms the state-of-the-art and can maintain stability in a dishonest environment.

3D printing techniques offer an effective method in fabricating complex radially multi-material structures. However, it is challenging for complex and delicate radially multi-material model geometries without supporting structures, such as tissue vessels and tubular graft, among others. In this work, we tackle these challenges by developing a polar digital light processing technique which uses a rod as the printing platform. The 3D model fabrication is accomplished through line projection. The rotation and translation of the rod are synchronized to project and illuminate the photosensitive material volume. By controlling the distance between the rod and the printing window, we achieved the printing of tubular structures with a minimum wall thickness as thin as 50 micrometers. By controlling the width of fine slits at the printing window, we achieved the printing of structures with a minimum feature size of 10 micrometers. Our process accomplished the fabrication of thin-walled tubular graft structure with a thickness of only 100 micrometers and lengths of several centimeters within a timeframe of just 100 s. Additionally, it enables the printing of axial multi-material structures, thereby achieving adjustable mechanical strength. This method is conducive to rapid customization of tubular grafts and the manufacturing of tubular components in fields such as dentistry, aerospace, and more.

Exosomes derived from mesenchymal stem cells (MSCs) have demonstrated regenerative potential for cell-free bone tissue engineering, nevertheless, certain challenges, including the confined therapeutic potency of exosomes and ineffective delivery method, are still persisted. Here, we confirmed that hypoxic precondition could induce enhanced secretion of exosomes from stem cells from human exfoliated deciduous teeth (SHEDs) via comprehensive proteomics analysis, and the corresponding hypoxic exosomes (H-Exo) exhibited superior potential in promoting cellular angiogenesis and osteogenesis via the significant up-regulation in focal adhesion, VEGF signaling pathway, and thyroid hormone synthesis. Then, we developed a platform technology enabling the effective delivery of hypoxic exosomes with sustained release kinetics to irregular-shaped bone defects via injection. This platform is based on a simple adsorbing technique, where exosomes are adsorbed onto the surface of injectable porous poly(lactide-co-glycolide) (PLGA) microspheres with bioinspired polydopamine (PDA) coating (PMS-PDA microspheres). The PMS-PDA microspheres could effectively adsorb exosomes, show sustained release of H-Exo for 21 days with high bioactivity, and induce vascularized bone regeneration in 5-mm rat calvarial defect. These findings indicate that the hypoxic precondition and PMS-PDA porous microsphere-based exosome delivery are efficient in inducing tissue regeneration, hence facilitating the clinical translation of exosome-based therapy.

Cross-IoT infrastructure access frequently occurs when performing tasks in a distributed computing infrastructure of a cyber-physical system (CPS). The access control technology that ensure secure access cross-IoT infrastructure usually automatically establish relationships between user-attribute/role-permission. How to efficiently determine whether an automatic authorization access control state satisfies the safety and availability requirements of a system is a huge challenge. Existing work often focuses on a single aspect of safety or availability, while ignoring the differences between permissions and the differences between users. In this paper, we first propose a fine-grained personalization policy that takes into account the specificity of permissions/users and describes the safety, availability and efficiency requirements of an access control system in CPS. Second, we define a Personalization Policy Checking (PPC) Problem to determine whether a given personalization policy is satisfied in an access control state. We give the computational complexity of the PPC problem in different subcases, and show that it is NP-complete in general. Third, we design a binary genetic search algorithm, whose improvements mainly include continuous update and selection of the best chromosomes in the population for iteration, and exploring and determining the optimal crossover and mutation probabilities, thereby improving the convergence efficiency of the algorithm. Finally, simulation results show the effectiveness of our proposed algorithm, which is especially fit for the case that the computational overhead is even more important than the accuracy in a large-scale CPS system.

Maxillary sinus augmentation is critical to oral implantology, particularly in some cases. The morphology of the sinus floor reflects the lifting effect to a certain extent; however, there has been limited research on the morphology after sinus augmentation. The present study aims to investigate the relationship between residual bone height (RBH) and the morphology of the sinus floor and determine whether a correlation exists between the different evaluation classifications. Maxillary sinus floor augmentation procedures were performed in 56 patients and 68 teeth using the sinus crest approach (SCA). Imaging results obtained after one year of sinus augmentation were analyzed and simultaneously classified along the coronal plane, the sagittal plane, and the biplane (coronal-sagittal). The higher the RBH, the closer the result tends to be to A, A’, or type 1 (more satisfactory). There was a significant correlation between the three different evaluation classifications (p

Binary magnesium-silver (Mg-Ag) alloys were designed as antibacterial materials for biomedical implant applications. In the present study, we focused on the effects of extrusion (extrusion ratio (ER): 1, 7.1, and 72.2) and Ag content (Ag = 0, 3, and 6 wt.%) on the degradation of Mg-Ag alloys in vitro and in vivo via microstructure characterization and corrosion/degradation measurements. The results showed that the Ag promoted a galvanic reaction with the Mg matrix to accelerate degradation or formed a protective oxide mesh texture to inhibit degradation, especially in vivo. Ag might also be beneficial for product crystallization, biomineralization, and organic matter deposition. For pure Mg, extrusion produced a more refined grain and decreased the degradation rate. For the Mg-Ag alloys, a low extrusion ratio (7.1) accelerated the degradation caused by the increase in the proportion of the precipitate. This promoted the release of Mg2+ and Ag+, which led to more deposition of organic matter and calcium phosphate, but also more H2 bubbles, which led to disturbance of product deposition in some local positions or even inflammatory reactions. Extrusion at a higher ratio (72.2) dissolved the precipitates. This resulted in moderate degradation rates and less gas production, which promoted osteogenesis without an obvious inflammation reaction.

Dental pulp necrosis are serious pathologic entities that causes tooth nutrition deficiency and abnormal root development, while regeneration of functional pulp tissue is of paramount importance to regain tooth vitality. However, existing clinical treatments, which focus on replacing the necrotic pulp tissue with inactive filling materials, fail to restore pulp vitality and functions, thus resulting in a devitalized and weakened tooth. Currently, dental pulp regeneration via stem cell-based therapy for pulpless teeth has raised enormous attention to restore the functional pulp. Here, a novel design of injectable simvastatin (SIM) functionalized gelatin methacrylate (GelMA) cryogel microspheres (SMS) loaded with stem cells from human exfoliated deciduous teeth (SHEDs) was established to refine SHEDs biological behaviors and promote in vivo vascularized pulp-like tissue regeneration. In this system, SIM encapsulated poly (lactide-co-glycolide) (PLGA) nanoparticles were incorporated into GelMA cryogel microspheres via cryogelation and O1/W/O2 emulsion method. SMS with sustained release of SIM promoted SHEDs adhesion, proliferation and exhibited cell protection properties during the injection process. Furthermore, SMS enhanced SHEDs odontogenic differentiation and angiogenic potential, and SHEDs loaded SMS (SHEDs/SMS) are beneficial for human umbilical vein endothelial cells (HUVECs) migration and angiogenesis, demonstrating their potential for use in promoting vascularized tissue regeneration. SHEDs/SMS complexes were injected into cleaned human tooth root segments for subcutaneous implantation in nude mice. Our results demonstrated that SHEDs/SMS could induce vessel-rich pulp-like tissue regeneration in vivo and that such an injectable nano-in-micro multistage system for the controlled delivery of bioactive reagents would be suitable for clinical application in endodontic regenerative dentistry.

In dentistry, zirconia implants have emerged as a promising alternative for replacing missing teeth due to their superior aesthetic performance and chemical stability. To improve the osseointegration of zirconia implants, modifying their surface with hierarchical micro/nanotopography and bioactive chemical composition are two effective ways. In this work, a microscale topography was prepared on a zirconia surface using hydrofluoric acid etching, and then a 50 nm TiO2 nanocoating was deposited via atomic layer deposition (ALD). Subsequently, an annealing treatment was used to transform the TiO2 from amorphous to anatase and simultaneously generate nanoscale topography. Various investigations into the coating surface morphology, topography, wettability, and chemical composition were carried out using scanning electron microscopy, white light interferometry, contact-angle measurement, X-ray diffraction, and X-ray photoelectron spectroscopy. In addition, in vitro cytocompatibility and osteogenic potential performance of the coatings were evaluated by human bone marrow mesenchymal stem cells (hBMSCs), and in vivo osseointegration performance was assessed in a rat femoral condyle model. Moreover, the possible mechanism was also investigated. The deposition of TiO2 film with/without annealing treatment did not alter the microscale roughness of the zirconia surface, whereas the nanotopography changed significantly after annealing. The in vitro studies revealed that the anatase TiO2 coating with regular wavelike nanostructure could promote the adhesion and proliferation of osteoblasts and further improve the osteogenic potential in vitro and osseointegration in vivo. These positive effects may be caused by nanoscale topography via the canonical Wnt/β-catenin pathway. The results suggest that using ALD in combination with annealing treatment to fabricate a nanotopographic TiO2 coating is a promising way to improve the osteogenic properties of zirconia implants.

Generating differentially private synthetic human mobility trajectories from real trajectories is a commonly used approach for privacy-preserving trajectory publishing. However, existing synthetic trajectory generation methods suffer from the drawbacks of poor scalability and suboptimal privacy–utility trade-off, due to continuous spatial space, high dimentionality of trajectory data and the suboptimal noise addition mechanism. To overcome the drawbacks, we propose DP-CSM, a novel differentially private trajectory generation method using coreset clustering and the staircase mechanism, to generate differentially private synthetic trajectories in two main steps. Firstly, it generates generalized locations for each timestamp, and utilizes coreset-based clustering to improve scalability. Secondly, it reconstructs synthetic trajectories with the generalized locations, and uses the staircase mechanism to avoid the over-perturbation of noises and maintain utility of synthetic trajectories. We choose three state-of-the-art clustering-based generation methods as the comparative baselines, and conduct comprehensive experiments on three real-world datasets to evaluate the performance of DP-CSM. Experimental results show that DP-CSM achieves better privacy–utility trade-off than the three baselines, and significantly outperforms the three baselines in terms of efficiency.

Osmotic stress is a severe condition frequently encountered by microorganisms; however, there is limited knowledge on the influence of hyperosmotic stress on the growth, development and pathogenicity of phytopathogenic fungi. Here, three osmotic conditions (0.4 M NaCl, 0.4 M KCl, and 0.6 M sorbitol supplemented in potato dextrose agar medium) were used to identify the effect of osmotic stress on the growth, development and pathogenicity of Setosphaeria turcica which is a plant pathogenic fungus and causes northern corn leaf blight disease in maize, sorghum, and related grasses. In osmotic stress, the growth rate of mycelium was decreased, and the number of vesicular structures and flocculent secretion outside the hypha cell wall were significantly increased. The qRT-PCR results showed that the osmotic stress quickly activated the HOG-MAPK pathway, up-regulated the expression of the downstream genes, and these genes were most highly expressed within 30 min of exposure to osmotic stress. Furthermore, the germination rate and the yield of conidia were significantly higher under osmotic stress than in the control. A pathogenicity analysis confirmed that pathogenicity of the conidia which were cultured under osmotic stress was significantly enhanced. By analyzing the knock-out mutants of an osmotic stress responsed gene StFPS1, an aquaglyceroporin downstream of the HOG-MAPK pathway, we found that StFPS1 was involved in the formation of appressorium and penetration peg, which affected the penetration ability of S. turcica. In summary, our work explained the correlation between osmotic stress and growth, development, and pathogenicity in S. turcica.

Background: In Saccharomyces cerevisiae, Msn2, which acts as a key transcription factor downstream the MAPK-HOG cascade pathway, also regulates the expression of genes related to stress responses. However, little is known about the regulation mechanisms of the transcription factor in Setosphaeria turcica. Results: In this study, a zinc finger DNA-binding protein, designated as StMSN2, was cloned from S. turcica. Sequencing results showed that StMSN2 had a 1752bp open reading frame (ORF), which was interrupted by an intron (135bp) and encoded a putative 538-amino acid protein. Phylogenetic analysis further revealed that StMsn2 was more closely related to Msn2 of Aspergillus parasiticus. StMSN2 was cloned into the pET-28a vector with His (Histidine) tags and induced with 1mM IPTG (isopropyl-β-D-thiogalactoside) at 37°C. The recombinant His-tagged StMsn2 was purified, and a band of size approximately 58.8kDa was obtained. The high specificity of the polyclonal antibody Msn2-2 was detected with the StMsn2 protein from S. turcica and prokaryotic expression system, respectively. Conclusions: A new gene, named StMSN2, with 1617bp ORF was cloned from S. turcica and characterized using bioinformatics methods. StMsn2 was expressed and purified in a prokaryotic system. A polyclonal antibody, named Msn2-2, against StMsn2 with high specificity was identified.How to cite: Lv R, Liu Y, Gong X, et al. Expression and purification of the transcription factor StMsn2 from Setosphaeria turcica in Escherichia coli. Electron J Biotechnol 2019;40. https://doi.org/10.1016/j.ejbt.2019.04.008 Keywords: Antigenic epitopes, Helminthosporium turcicum, Heterologous expression, Maize, Open reading frame, Plant fungal pathogen, Protein purification, Setosphaeria turcica, Stress responses, Transcription factor, Zinc finger

Strategic users in a service exchange application of crowdsensing are apt to exhibit malicious behaviors such as greed, free-ride, and attack, resulting in the phenomenon that no user is willing to serve others and low social utility is obtained in myopic equilibrium, which is considered as a service exchange contest dilemma. To address this issue, we propose a game-theoretic framework of multi-level two-sided rating protocol using all-pay contests to balance service request and service provision between users, in which a user is tagged with a multi-level rating to represent her social status, and she is encouraged to take the initiative to be a server and provide high-quality services to increase her rating. The two-sided rating update rule updates the ratings of both service requesters and service providers, and thus no one can always get services without providing services. By quantifying necessary and sufficient conditions for a sustainable multi-level two-sided rating protocol, we formulate the problem of selecting the optimal design parameters to maximize the social utility among all sustainable multi-level two-sided rating protocols, and design a low-complexity algorithm to select optimal design parameters via a two-stage procedure in an alternate manner. Finally, the extensive evaluation results demonstrate how intrinsic parameters impact on recommended strategies, design parameters, as well as the performance gain of the proposed rating protocol.

The emerging Industrial Internet of Things (IIoT) provides industries with an opportunity to collect, aggregate, and analyze data from sensors, including motion control, machine-to-machine communication, predictive maintenance, smart energy grid, big data analysis, and other smart connected medical systems. The physical systems and the cyber systems are organically integrated, forming an interdependent IIoT. This system provides us with enormous advantages, but at the same time, it also introduces the main safety challenges in the design and operation phase. To exploit the security threats of IIoT systems, in this paper, we propose a novel security-by-design approach for interdependent IIoT environments across two different levels, namely, theory modeling and runtime simulation. Our method theoretically analyzes the cascading failure dynamics of the intentional attack network. Simultaneously, we verified the theoretical results through simulations and gave the risk factors that affect the system’s security to mitigate potential security attack threats. Besides, we prove its applicability through comparative simulation experiments to study application environments that rely on IIoT, which shows that our method helps identify risk factors and mitigate IIoT attacks’ mechanism.

Immunization strategies on complex networks are effective methods to control the spreading dynamics on complex networks, which change the topology and connectivity of the underlying network, thereby affecting the dynamics process of propagation. Here, we use a non-Markovian threshold model to study the impact of immunization strategies on social contagions, in which the immune index greater than (or equal to) 0 corresponds to targeted (random) immunization, and when the immune index is less than 0, the probability of an individual being immunized is inversely related to the degree of the individual. A generalized edge-based compartmental theory is developed to analyze the dynamics of social contagions under immunization, and theoretical predictions are very consistent with simulation results. We find that increasing the immune index or increasing the immune ratio will reduce the final adoption size and increase the outbreak threshold, in other words, make the residual network after immunization not conducive to social contagions. Interestingly, enhancing the network heterogeneity is proved to help improve the immune efficiency of targeted immunization. Besides, the dependence of the outbreak threshold on the network heterogeneity is correlated with the immune ratio and immune index.

The thermal fatigue crack initiation and propagation behavior of SiCp/A356 composites which is produced by stirring casting were studied. Specimens with a V-shaped notch were used in the thermal fatigue experiment. Optical microscope (OM) and scanning electron microscope (SEM) were used to observe the crack growth. Crack initiated at the notch tip after about 150 cycles of heating and cooling from room temperature to 250 °C. The crack propagation stage was dominated during the whole crack growth. Cracks mainly propagated along the interface of particle and through the matrix. The propagation stage experienced the cycle of slow propagation and fast propagation, and a Step-Like shape was observed during the crack growth process. The micro cracks appeared in the interface of particle and the matrix after repeated thermal cycles, and induced fast propagation by coalescing with the main cracks. Distributions of the particle play an important role in hindering thermal fatigue crack propagation. Hardness of the composites decreased with the increase of the number of cycles, and decreased by 46.1% after thermal 290 cycles. Keywords: SiCp/A356 composites, Thermal fatigue crack, Stirring casting, Crack propagation, Crack growth

Temperature evolution of the train brake disc during high-speed braking was investigated using in situ experiments, theoretical analysis, and finite element modeling. The experimental results show that the temperature distribution on the friction surface experienced the formation of a hot ring first, then expansion and duration of the hot ring. Alternative hot spot and cold zone were observed on the friction surface, which is attributed to the local contact in the friction couple and heterogeneous heat dissipation condition in the disc. The corresponding maximum temperature in the disc increased rapidly first, kept stable then, and decrease slowly in the end. The one-dimensional heat conduction equation was applied to predict the maximum temperature variation and was found to be in agreement with the experimental results. Furthermore, the maximum temperature evolution and the temperature distribution of the disc at the braking time of 45 s were simulated by the finite element method, which is satisfactory. In additional, the temperature variation caused the corresponding fluctuation of instantaneous frictional coefficient and thermal stress distribution in the disc, which results in the thermal damages.

With the advent of the Internet of Everything era, the Industrial Internet is increasingly showing mutual integration and development. Its core framework, the industrial CPS (Cyber-Physical Systems), has received more and more attention and in-depth research in recent years. These complex industrial CPS systems are usually composed of multiple interdependent sub-networks (such as physical networks and control networks, etc.). Minor faults or failure behaviors between sub-networks may cause serious cascading failure effects of the entire system. In this paper, we will propose a security scheme based on intranal-adding links in the face of the integrated and converged industrial CPS system environment. Firstly, by calculating the size of the largest connected component in the entire system, we can compare and analyze industrial CPS systems’ security performance under random attacks. Secondly, we compare and analyze the risk of cascading failure between integrated industrial CPS systems under different intranal-adding link strategies. Finally, the simulation results verify the system security strategy’s effectiveness under different strategies and show a relatively better exchange strategy to enhance the system’s security. In addition, this paper’s research work can help us design how to further optimize the interdependent industrial CPS system’s topology to cope with the integrated and converged industrial CPS system environment.

In the real Internet of Everything scenario, many large-scale information systems can be converted into interdependent sensor networks, such as smart grids, smart medical systems, and industrial Internet systems. These complex systems usually have multiple interdependent sensor networks. Small faults or failure behaviors between networks may cause serious cascading failure effects of the entire system. Therefore, in this paper, we will focus on the security of interdependent sensor networks. Firstly, by calculating the size of the largest functional component in the entire network, the impact of random attacks on the security of interdependent sensor networks is analyzed. Secondly, it compares and analyzes the impact of cascading failures between interdependent sensor networks under different switching edge strategies. Finally, the simulation results verify the effect of the security of the system under different strategies, and give a better exchange strategy to enhance the security of the system. In addition, the research work in this article can help design how to further optimize the topology of interdependent sensor networks by reducing the impact of cascading failures.

A pathogenic mutant, BCG183, was obtained by screening the T-DNA insertion library of Botrytis cinerea. A novel pathogenicity-related gene BcKMO, which encodes kynurenine 3-monooxygenase (KMO), was isolated and identified via thermal asymmetric interlaced PCR, bioinformatics analyses, and KMO activity measurement. The mutant BCG183 grew slowly, did not produce conidia and sclerotia, had slender hyphae, and presented enhanced pathogenicity. The phenotype and pathogenicity of the BcKMO-complementing mutant (BCG183/BcKMO) were similar to those of the wild-type (WT) strain. The activities of polymethylgalacturonase, polygalacturonase, and toxins were significantly higher, whereas acid production was significantly decreased in the mutant BCG183, when compared with those in the WT and BCG183/BcKMO. Moreover, the sensitivity of mutant BCG183 to NaCl and KCl was remarkably increased, whereas that to fluconazole, Congo Red, menadione, H2O2, and SQ22536 and U0126 [cAMP-dependent protein kinase (cAMP) and mitogen-activated protein kinase (MAPK) signaling pathways inhibitors, respectively] were significantly decreased compared with the other strains. Furthermore, the key genes involved in the cAMP and MAPK signaling pathways, Pka1, Pka2, PkaR, Bcg2, Bcg3, bmp1, and bmp3, were significantly upregulated or downregulated in the mutant BCG183. BcKMO expression levels were also upregulated or downregulated in the RNAi mutants of the key genes involved in the cAMP and MAPK signaling pathways. These findings indicated that BcKMO positively regulates growth and development, but negatively regulates pathogenicity of B. cinerea. Furthermore, BcKMO was found to be involved in controlling cell wall degrading enzymes activity, toxins activity, acid production, and cell wall integrity, and participate in cAMP and MAPK signaling pathways of B. cinerea.

Scanning microarc oxidation (SMAO) is a coating process which is based on conventional microarc oxidation (MAO). The key difference is that deposition in SMAO is achieved by using a stainless steel nozzle to spray an electrolyte stream on the substrate surface as opposed to immersing the workpiece in an electrolyzer. In the present study, SMAO discharge characteristics, coating morphology, and properties are analyzed and compared to results obtained from MAO under similar conditions. Results show that MAO and SMAO have comparable spark and microarc lifetimes and sizes, though significant differences in incubation time and discharge distribution were evident. Results also showed that the voltage and current density for MAO and SMAO demonstrate similar behavior but have markedly different transient and steady-state values. Results obtained from coating A356 aluminum sheet show that oxide thickness and growth rate in SMAO are strongly dependent on interelectrode spacing and travel speed. Analysis of the SMAO coating morphology and structure showed that a denser and slightly harder layer was deposited in comparison to MAO and is attributed to reduced porosity and increased formation of α-Al2O3. Preliminary results indicate that SMAO represents a viable process for coating of aluminum surfaces.

Due to the low density and high temperature resistance, the SiCp/A356 composites have great potential for weight reduction and braking performance using the brake disc used in trains and automobiles. But the friction coefficient and braking performance are not stable in the braking process because of temperature rising. In this paper, friction and wear behaviors of SiCp/A356 composite against semimetallic materials were investigated in a ring-on-disc configuration in the temperature range of 30°C to 300°C. Experiments were conducted at a constant sliding speed of 1.4 m/s and an applied load of 200 N. Worn surface, subsurface, and wear debris were also examined by using SEM and EDS techniques. The third body films (TBFs) lubricated wear transferred to the third body abrasive wear above 200°C, which was a transition temperature. The friction coefficient decreased and weight of semimetallic materials increased with the increase of temperature and the temperature had almost no effect on the weight loss of composites. The dominant wear mechanism of the composites was microploughing and slight adhesion below 200°C, while being controlled by cutting grooves, severe adhesion, and delamination above the 200°C.

Achieving the uniform distribution of reinforcement particles in MMCs is very important for the effect of stirring parameters and the flow action of the melt, which should be known. The effect of stirring parameters on the distribution of SiC particles in SiCp/A356 composites was studied by the experimental and numerical methods in this paper. The experimental results show the SiC distribution with different stirring parameters. In addition, the effects of the fluid velocity and volume fraction of SiC particle at different position of crucible on the SiC distribution were analyzed by numerical simulation. The velocity magnitude, axial velocity, and radial velocity were analyzed to explain theoretically the particle distribution. The shearing force, moments, and stirring power of the stirring rod were simulated based on CFD code. The numerical results show that the stirring temperature is lower, the shearing force is greater, the stirring time is longer, and particle dispersion gets better. On the other hand, the higher the stirring speed is, the more uniform the radial and axial flow are, and the better the particles were dispersed. The numerical results were in good agreement with the experimental data.

Epidemiological studies have suggested inconclusive associations between 25-hydroxyvitamin D and total cancer incidence and mortality. The aim of this study was to quantitatively assess these associations by combining results from prospective cohort studies. A systematic literature search was implemented in PubMed and Scopus databases in April 2019. Comparing the highest with the lowest categories, the multivariate-adjusted relative risks (RRs) and the corresponding 95% confidence intervals (CIs) were pooled using a random-effects model. A trend estimation was performed using a two-stage, dose-response, meta-analysis method. Twenty-three independent prospective studies were included for data synthesis. Eight studies investigated the association between 25-hydroxyvitamin D and the risk of cancer incidence (7511 events and 70,018 participants), and the summary estimate showed that 25-hydroxyvitamin D is marginally associated with cancer risk (Summary RR = 0.86; 95% CI: 0.73, 1.02; I2 = 70.8%; p = 0.001). Sixteen studies investigated the association between 25-hydroxyvitamin D and the risk of cancer mortality (8729 events and 101,794 participants), and a higher 25-hydroxyvitamin D concentration was inversely associated with the risk of cancer mortality (Summary RR = 0.81; 95% CI: 0.71, 0.93; I2 = 48.8%, p = 0.012). Dose-response analysis indicated that the risk of cancer incidence was reduced by 7% (RRs = 0.93; 95% CI: 0.91, 0.96), and the risk of cancer mortality was reduced by 2% (RRs = 0.98; 95% CI: 0.97, 0.99), with each 20 nmol/L increment of 25-hydroxyvitamin D concentration. This meta-analysis provides evidence that a higher 25-hydroxyvitamin D concentration is associated with a lower cancer incidence and cancer mortality.

Calcium phosphate- (CaP-) based composite scaffolds have been used extensively for the bone regeneration in bone tissue engineering. Previously, we developed a biomimetic composite nanofibrous membrane of gelatin/β-tricalcium phosphate (TCP) and confirmed their biological activity in vitro and bone regeneration in vivo. However, how these composite nanofibers promote the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is unknown. Here, gelatin/β-TCP composite nanofibers were fabricated by incorporating 20 wt% β-TCP nanoparticles into electrospun gelatin nanofibers. Electron microscopy showed that the composite β-TCP nanofibers had a nonwoven structure with a porous network and a rough surface. Spectral analyses confirmed the presence and chemical stability of the β-TCP and gelatin components. Compared with pure gelatin nanofibers, gelatin/β-TCP composite nanofibers caused increased cell attachment, proliferation, alkaline phosphatase activity, and osteogenic gene expression in rat BMSCs. Interestingly, the expression level of the calcium-sensing receptor (CaSR) was significantly higher on the composite nanofibrous scaffolds than on pure gelatin. For rat calvarial critical sized defects, more extensive osteogenesis and neovascularization occurred in the composite scaffolds group compared with the gelatin group. Thus, gelatin/β-TCP composite scaffolds promote osteogenic differentiation of BMSCs in vitro and bone regeneration in vivo by activating Ca2+-sensing receptor signaling.

The glass infiltration technique was employed for surface modification of zirconia implants in this study. The prepared glassinfiltrated zirconia with low infiltrating temperature showed excellent mechanical properties and enough infiltrating layer. The zirconia substrate was pre-sintered at 1,200°C and the glass infiltration depth reached 400 μm after infiltrating at 1,200°C for 10 h. The infiltrating glass has good wetting ability, thermal expansion match and good chemical compatibility with the zirconia substrate. Indentation fracture toughness and flexural strength of the dense sintered glass-infiltrated zirconia composite are respectively 5.37±0.45 MPa•m1/2 and 841.03±89.31 MPa. Its elasticity modulus is 163.99±7.6 GPa and has about 500 μm infiltrating layer. The glass-infiltrated zirconia can be acid etched to a medium roughness (1.29±0.09 μm) with a flexural strength of 823.65±87.46 MPa, which promotes cell proliferation and has potential for dental implants. [ABSTRACT FROM AUTHOR]