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In a large number of real-life scenes and practical applications, 3D object detection is playing an increasingly important role. We need to estimate the position and direction of the 3D object in the real scene to complete the 3D object detection task. In this paper, we propose a new network architecture based on VoteNet to detect 3D point cloud targets. On the one hand, we use channel and spatial dual-domain attention module to enhance the features of the object to be detected while suppressing other useless features. On the other hand, the SIFT operator has scale invariance and the ability to resist occlusion and background interference. The PointSIFT module we use can capture information in different directions of point cloud in space, and is robust to shapes of different proportions, so as to better detect objects that are partially occluded. Our method is evaluated on the SUN-RGBD and ScanNet datasets of indoor scenes. The experimental results show that our method has better performance than VoteNet.

In the previous literatures, we found that the bioactivity of prolyl hydroxylase-2 (PHD-2) that can mediate the response to hypoxia in the process of sports was suppressed by JTZ-951 and its analogues. To investigate the interaction mechanism, the complexes of PHD-2 with its inhibitors were explored by using molecular docking, molecular dynamics simulation, and binding free energy decomposition calculation methods. The results of energy decomposition are consistent with available experimental results and suggest that the binding affinity of compound 14 located in the activity region of PHD-2 is more stable than those of compound 4 and compound 9. Due to the propyl benzene moiety of compound 14, compound 14 can exactly locate in the activity pocket of PHD-2 (residues of ASP-254, TYR-303, TYR-310, ILE-327, LEU-343, GLU-375, VAL-376 and ARG-411 are the binding sites in PHD-2-compound 14 complex) via the binding energy calculation between all residues of PHD-2 and inhibitors. In addition, compound 4 and 9 are far away from the activity core of PHD-1, resulting in the lower inhibitory activity. These results suggested that the propyl benzene group in compound 14 had a greater impact on the inhibitory activity. This work will improve the development of novel inhibitors targeting PHD-2.

Medical images are an important means to assist doctors in making judgments. For the problem that it is difficult to segment various complex tissue structures in medical images, we propose a segmentation method based on feature aggregation. Firstly, our method adopts VoVNet as the backbone and outputs multi-scale features. Secondly, we use the multi-scale features aggregation module to extract context information fully. Finally, we adopt an attention module to consider the relevance of each spatial and channel. Through the experiments conduct on two datasets, the proposed model scores a dice coefficient of 90.90% and 86.10%. Results show that our network can segment the target area well in the gray image and RGB image and outperforms the existing methodologies. We provide a download link to our method.

A new luminescent metal-organic framework [Cu(Hbcb)(PYTPY)] (1) (H3bcb=3,5-bis((5′-carboxylbenzyl)oxy)benzoic acid, PYTPY=4′-(4-pyridyl)-2,2′:6′,2′'-terpyridine) has been successfully synthesized and structurally characterized by elemental analysis, IR and single crystal X-ray diffraction. Analysis revealed that an infinite one-dimensional ladder chain was created by offset face-to-face π-π stacking interactions between H3bcb and PYTPY. And it exhibits a three-dimensional supramolecular structure through the π-π stacking interactions between chains. The thermal stabilities and gas adsorption properties of complex 1 was studied. The experimental results show that complex 1 has good thermal stabilities and selective adsorption properties for CO2. The solid-state luminescent properties of MOFs have been measured carefully by ultraviolet irradiation at room temperature. Most noteworthy, complex 1 exhibits a high sensitivity for Fe3+ and nitrobenzene in ethanol solution with mixed metal ions and aromatic compounds, which makes it be a promising crystalline material as luminescent probe to Fe3+ and nitrobenzene. Importantly, complex 1 can keep its original framework and be reused in sensing experiments. The sensing mechanism has also been studied in detail.

Haemophilus parasuis , a symbiotic bacteria of upper respiratory tract of swine, is the etiological agent of Glasser's disease, which is characterized by fibrinous polyserositis. Emodin, exhibits antibacterial activity against H. parasuis , yet the action mode has not been fully understood. In present study, isobaric tag for relative and absolute quantification (iTRAQ) method was applied to analyze the global protein alteration of H. parasuis in response to 16 μg/mL Emodin. In total, 338 proteins exhibiting significant differential expressions were identified. It was speculated that, through application of bioinformatics analysis to theses differentially expressed proteins, Emodin mainly inhibited some key proteins expression of ABC transport system, carbohydrate metabolism pathway and bacterial cell division by inhibiting the ribosome synthesis, resulting in the growth inhibition of H. parasuis. Remarkably, nine virulence-associated proteins were detected differently expressed, further experiments revealed that after treatment with Emodin, H. parasuis could be inhibited to adhere to and invade into porcine kidney epithelial cells (PK-15 line) and exhibited increased sensitivity to serum complement in a concentration-dependent manner. Phagocytosis assay showed Emodin also could enhance phagocytic activity of porcine alveolar macrophages PAM to H. parasuis. These results indicated that Emodin also can attenuate virulence of H. parasuis and reduce infection. Biological significance The Glasser's disease caused by H. parasuis has become a typical bacterial disease and cause serious economic loss to the swine industry around the world. Antibiotics are extensively used to control the infection, but increasing antibiotic resistance has been a severe problem. Hence, novel treatment agents are needed. So far, few antibacterial agents were reported that could control H. parasuis infection. In the present study, the state-of-the-art quantitative proteomic technology was applied to uncover underlying action mechanism of Emodin. This study extends understanding of antibacterial effect of Emodin to H. parasuis at molecular level and provides useful information for further investigations. Moreover, our results provide theoretical foundation for the practical application of Emodin.

The MIC and MBC values of Shikuqin (SKQ) against 5 bacteria that readily cause diarrhea were measured by the broth micro dilution method. The castor oil-induced diarrhea method was used to evaluate the antidiarrheal activity. Intestinal transit and gastric emptying were also evaluated with normal and neostigmine-induced intestinal transit in rodents. In addition, the antidiarrheal activity of SKQ was assessed in vivo with isolated rabbit ileum. Xylene-induced ear edema was used to evaluate the anti-inflammatory activities in mice, while hot plate and writhing tests were performed to assess the analgesic effects. Senna decoction (0.3g/mL) was administered intragastrically to induce a rat model of diarrhea. Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect AQP4 mRNA, and Western blot was performed to quantify the protein level of AQP4 in the colon. SKQ exhibits remarkable antidiarrheal, anti-inflammatory and analgesic effects in the gastrointestinal tract disorders, and can therefore be developed as a promising antidiarrheal agent.

"Shikuqin" (SKQ) powder consists of three Chinese herbs: Punica granatum L, Sophora flavescens Ait, and Fraxinus rhynchophylla Hance. SKQ has been used for the treatment of diarrhea. In order to provide a comprehensive understanding of toxicity, the acute and sub-chronic toxicity and safety pharmacology of SKQ were evaluated in the present study. The result of the acute toxicity revealed that the LD50 of the valve was 28,379mg/kg.b.w, which was more than 5,000 mg/kg b.w. The 30-day sub-chronic toxicity test results revealed that compared with the control group, the clinical signs, hematology parameters and body weight of rats in each group had no significant differences. The viscera coefficient and histopathological examination results revealed that the SKQ powder could cause kidney and liver damage. In the safety pharmacology test, SKQ did not exhibit any toxicity to the central nervous system, cardiovascular system and respiratory system. In conclusion, SKQ powder could be considered safe for veterinary use.

Rotavirus (RV), belonging to Reoviridae family, is the leading cause of acute severe viral diarrhea in children (under 5 years old) and infant animals worldwide. Although vaccines are commonly used to prevent infection, episodes of diarrhea caused by RV frequently occur. Thus, this study was conducted to determine whether resveratrol had protective effects against RV infection in piglets. Following pretreatment with resveratrol dry suspension through adding into the basal diet for 3 weeks, the piglets were orally challenged with RV. We found that resveratrol could alleviate diarrhea induced by RV infection. Resveratrol-treatment inhibited the TNF-α production, indicating that the anti-RV activity of resveratrol may be achieved by reducing the inflammatory response. The IFN-γ level was elevated in 10mg/kg/d resveratrol-treated group and 30mg/kg/d resveratrol-treated group after RV infection. The ratios of CD4+/CD8+ in resveratrol-treated groups were the same as that in mock infected group, suggesting that resveratrol could maintain the immune function in RV-infected piglets. It was found that resveratrol could alleviate diarrhea induced by RV infection. These results revealed that resveratrol dry suspension could be a new control measure for RV infection.

MPP can be effective in the treatment of E. coli O101-induced diarrhea in mice. MPP can improve the weight loss caused by diarrhea, increase spleen and thymus indices, and reduce the diarrhea index. MPP can reduce the number of WBC, regulate the level of cytokines, and regulate the intestinal microbial flora. These data suggest that MPP is a promising candidate for treatment of E. coli-induced diarrhea in humans and animals.

Aim. Dermatophytosis is one of the main fungal diseases in humans and animals all over the world.Galla chinensis, a traditional medicine, has various pharmacological effects. The goal of this study was to evaluate the treatment effect ofGalla chinensissolution (GCS) on dermatophytosis-infected dogs (Microsporum canis,Microsporum gypseum, andTrichophyton mentagrophytes, resp.).Methods. The treatment effects of GCS were evaluated by mycological cure rates and clinical score comprised of three indices, including inflammation, hair loss, and lesion scale.Results. The results showed that, in the three models of dermatophytosis, GCS significantly (P<0.05) improved skin lesions and fungal eradication. GCS (10% and 5%) had higher efficacy compared to the positive control (Tujingpi Tincture). The fungal eradication efficacy exceeds 85% after treatment with GCS (10%, 5%, and 2.5%) on day 14.Conclusion. The GCS has antidermatophytosis effect in dogs, which may be a candidate drug for the treatment of dermatophytosis.