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There is still controversy about optimal dietary iodine intake as the Universal Salt Iodization policy enforcement in China. A modified iodine balance study was thus conducted to explore the suitable iodine intake in Chinese adult males using the iodine overflow hypothesis. In this study, thirty-eight apparently healthy males (19·1 (sd 0·6) years) were recruited and provided with designed diets. After the 14-d iodine depletion, daily iodine intake gradually increased in the 30-d iodine supplementation, consisting of six stages and each of 5 d. All foods and excreta (urine, faeces) were collected to examine daily iodine intake, iodine excretion and the changes of iodine increment in relation to those values at stage 1. The dose–response associations of iodine intake increment with excretion increment were fitted by the mixed effects models, as well as with retention increment. Daily iodine intake and excretion were 16·3 and 54·3 μg/d at stage 1, and iodine intake increment increased from 11·2 μg/d at stage 2 to 118·0 μg/d at stage 6, while excretion increment elevated from 21·5 to 95·0 μg/d. A zero iodine balance was dynamically achieved as 48·0 μg/d of iodine intake. The estimated average requirement and recommended nutrient intake were severally 48·0 and 67·2 μg/d, which could be corresponded to a daily iodine intake of 0·74 and 1·04 μg/kg per d. The results of our study indicate that roughly half of current iodine intakes recommendation could be enough in Chinese adult males, which would be beneficial for the revision of dietary reference intakes.

Lead (Pb) pollution is a global public health problem and adversely affects children health across a wide range of exposure levels. This study was conducted to evaluate the blood Pb levels (BLLs) of Chinese urban children aged 6−11 years and to describe the temporal variations from the China Nutrition and Health Survey (CNHS) in 2002 and 2012. A total of 2182 (1036 vs. 1146) Chinese urban children were selected in the CNHS 2002 and the CNHS 2012 using the multistage stratified cluster random and probability proportional to size sampling method. The BLLs of urban children were determined using inductively coupled plasma mass spectrometer (ICP-MS), whilst the prevalence of BLLs >50 µg/L and >100 µg/L were calculated, as well as the reference values (RV95s: the upper limit of 95% confidence interval of 95th percentile). From the CNHS 2002 to the CNHS 2021, the median BLLs of Chinese urban children had obviously changed from 67.4 µg/L to 36.9 µg/L, and the RV95s of BLLs had also descrended from 149.1 µg/L to 107.9 µg/L. In addition, the prevalence of BLLs with >50 µg/L and >100 µg/L had markedly decreased from 74.0% and 18.2% in the CNHS 2002 to 28.4% and 5.2% in the CNHS 2021. In conclusion, the BLLs of Chinese urban children had been definitely improved in the past 10 years. However, the findings of our study suggested that it is imperative to implement the monitoring and evaluating and to continue control Pb exposure.

Background Knowledge of the physiologic requirement for iron, the core index for the formulation of a dietary reference intake (DRI), is of great importance for the health of a pregnant woman and her fetus, and can help a mother accurately plan her iron supplementation. However, direct measurements of the physiologic requirement for iron during pregnancy are still lacking. Method Eleven women of reproductive age from Hebei Province, China, who planned to become pregnant in the near future, were enrolled between January and March 2015 and included in the final analysis. Subjects participated in a 2-week metabolic trial in which they consumed 50 mg of the stable isotope 58Fe, and were then followed for ~ 2 years. The abundance of 58Fe and the total iron concentration in the circulation were measured using Multi-collector Inductively-Coupled Plasma Mass Spectrometry and Atomic Absorption Spectroscopy, respectively. The physiologic requirement for iron during pregnancy was then calculated by the formula derived from our previously published work. Results The mean physiologic requirement for iron in the 11 subjects, across their entire pregnancies, was 3.05 mg.d− 1 in total and 44.0 μg.kg− 1.d− 1 after adjustment for body mass. The physiologic requirements for iron in the first, second, and third trimesters were 2.04 mg.d− 1, 3.26 mg.d− 1, and 4.13 mg.d− 1, respectively. When adjusted for body mass, the physiologic requirements for iron in different trimesters were 32.3 μg.kg− 1.d− 1, 46.9 μg.kg− 1.d− 1, and 55.7 μg.kg− 1.d− 1, respectively. Conclusion We preliminarily explored the physiologic requirement for iron in pregnant women. The data demonstrated that pregnant women needed about twice iron than non-pregnant women. This research may be helpful for the design of future studies and the modification of iron DRIs. Trial registration ChiCTR, ChiCTR-OCH-14004302. Registered 14 February 2014, http://www.chictr.org.cn/showproj.aspx?proj=5267

Background Appropriate iodine intake for adults is essential to reduce the prevalence of thyroid diseases, but there is little research data on iodine requirement of Chinese population. This study aimed to explore the iodine requirement of young adults to maintain a healthy status based on ‘overflow theory’. Methods Iodine-balance experiment has been performed in this project. We conducted an 18-day study consisted of a 6-day acclimation period and 3 consecutive experimental stages in 37 Chinese healthy young adults (23 female and 14 male). Each stage was consumed for 4 days. Strictly-controlled low-iodine intake diets were provided for adults in the first period, an egg or 125mL milk was added in the second and third period, respectively. The dietary samples, 24-h urine specimens and faeces of volunteers were collected daily for assessment of iodine intake and excretion in volunteers. Results Mean values of iodine intake (22.7±3.6, 35.1±3.7, and 52.2±3.8μg/d), excretion (64.7±13.9, 62.3±12.6, and 94.3±14.5μg/d) and iodine balance (-35.2±19.5, -21.0±19.8, and -33.5±26.9μg/d) were significantly different among three periods for male (PP < 0.001 for all). No significant difference was observed among the 3 periods for female in the iodine balance (-30.5±9.3, -25.9±7.3, and -27.6±12.1μg/d). The linear regression equation of iodine excretion on iodine intake was Y=0.979X+37.04 (male) and Y=0.895X+31.48 (female). Compared with stage 2, iodine excretion increments in stage 3 had exceeded the iodine intake increment for men. The ratio of increment was 1.675 for male when the average iodine intake was 52.2μg/d in stage 3. When the iodine excretion increment equaled to the iodine intake increment, the daily iodine intake of men was 47.0μg. Conclusion We have evaluated the iodine requirement of young adults in southern China based on overflow theory. Our results indicate the lower limit of iodine requirement for Chinese young men is 47.0μg/d. The trial was registered at www.chictr.org.cn as ChiCTR1800014877.

Additional file 2: Table S2. Estimation of Maternal Weight Gain during Pregnancy.

Additional file 1: Table S1. The Circulation Rate of Non-pregnant Women.

Optimal vitamin D (vitD) status is beneficial for both pregnant women and their newborns. The aim of this study was to evaluate the vitamin D status of Chinese pregnant women in the latest China Nutrition and Health Surveillance (CNHS) 2015–2017, analyze the risk factors of vitamin D deficiency (VDD), and compare them with those in CNHS 2010–2012. Serum 25 hydroxyvitamin D (25(OH)D) was measured by ELISA method. City type, district, latitude, location, age, vitamin D supplements intake, education, marital status, annual family income, etc., were recorded. The median 25(OH)D concentration was 13.02 (10.17–17.01) ng/mL in 2015–2017, and 15.48 (11.89–20.09) ng/mL in 2010–2012. The vitamin D sufficient rate was only 12.57% in 2015–2017, comparing to 25.17% in 2010–2012. The risk factors of vitamin D inadequacy (25(OH)D &lt, 20 ng/mL) in 2015–2017 were not exactly consistent with that in 2010–2012. The risk factors included season of spring (p &lt, 0.0001) and winter (p &lt, 0.001), subtropical (p &lt, 0.001), median (p &lt, 0.0001) and warm temperate zones (p &lt, 0.0001), the western (p = 0.027) and the central areas (p = 0.041), while vitD supplements intake (p = 0.021) was a protective factor in pregnant women. In conclusion, vitD inadequacy is very common among Chinese pregnant women. We encourage pregnant women to take more effective sunlight and proper vitD supplements, especially for those from the subtropical, warm and medium temperate zones, the western and the central, and in the seasons of spring and winter.

Zinc is an essential trace element for growth and development in children, but zinc deficiency is a serious nutritional problem worldwide. Our study aimed to assess the zinc status of school-age children living in rural areas of China and to examine the change of zinc status based on the China Nutrition and Health Survey 2002 and 2012. We used the probability proportional to size sampling method for subject selection, and a total of 3407 school-age children were included in this study. Zinc status was assessed by three items of indicators recommended by the World Health Organization (WHO), the United Nations Children's Fund (UNICEF), the International Atomic Energy Agency (IAEA), and the International Zinc Nutrition Consultative Group (IZiNCG). The concentration of serum zinc was 718.2 μg/L, and 44.4% of children being zinc deficiency in 2002, while 846.8 μg/L and 10.4% in 2012. Zinc intake was 7.8 mg/day with a 7.6% inadequate zinc intake in 2002, together with 6.9 mg/day and 38.2% in 2012. Height-for-age Z score was -1.06 and 19.1% of children being stunting in 2002, as well as -0.15 and 6.8% in 2012. In conclusion, the zinc status of school-age children living in rural areas of China has been significantly improved in addition to zinc intake over the past 10 years. However, the zinc deficiency still observed in poor rural areas of China in 2012. In addition, we suggested that the zinc bioavailability should be taken into account when assessing zinc status in population.

In the present work, we evaluated the three generation reproductive toxicity of the genetically modified rice with insectresistant cry1Ac and sck genes. 120 Sprague-Dawley (SD) rats were divided into three groups which were fed with genetically modified rice diet (GM group), parental control rice diet (PR group) and AIN-93 control diet (both used as negative control) respectively. Bodyweight, food consumption, reproductive data, hematological parameters, serum chemistry, relative organ weights and histopathology for each generation were examined respectively. All the hematology and serum chemistry parameters, organ/body weight indicators were within the normal range or no change to the adverse direction was observed, although several differences in hematology and serum chemistry parameters (WBC, BUN, LDH of male rat, PLT, PCT, MPV of female rats), reproductive data (rate of morphologically abnormal sperm) were observed between GM rice group and two control groups. No macroscopic or histological adverse effects were found or considered as treatment-related, either. Overall, the three generation study of genetically modified rice with cry1Ac and sck genes at a high level showed no unintended adverse effects on rats's reproductive system.

This study assessed the bioavailability and biological incorporation of nonheme iron from staple food diets in healthy young urban Chinese women and determined the relevant effects of typical regional patterns of staple foods in South and North China.Twenty-two young urban Chinese women aged 20-23 years were enrolled and randomly allocated to two groups, with rice (rice group) and steamed buns (steamed buns group) as the staple food, respectively. Each participant received three meals daily containing approximately 3.25 mg of stable 57FeSO4 for 2 consecutive days, along with daily intravenous injection of approximately 2.0 mg of 58FeSO4. Nonheme iron absorption and infused iron incorporation rates were assayed.In all participants, the rice group, and the buns group, nonheme iron intake was 7.2±1.6, 5.9±0.6, and 8.4±1.2 mg, respectively; mean 57FeSO4 absorption rate was 22.2%±9.6%, 22.2%±10.6%, and 22.2%±8.9%, respectively; and the mean infused 58FeSO4 incorporation rate was 91.6%±8.2%, 93%±7.3%, and 90%±9.1%, respectively. No substantial differences existed in the nonheme iron intakes and the 57FeSO4 absorption and 58FeSO4 incorporation rates between the rice and buns groups (all p0.05).The bioavailability and incorporation rates of nonheme iron from representative comprehensive Chinese diets in healthy young urban Chinese women were evaluated. Our results can facilitate the establishment of dietary reference intake for iron in Chinese women.

Accurately assessing energy expenditure (EE) of people with different body weight is essential to facilitate weight management. The aim of this study was to measure basal energy expenditure (BEE), resting energy expenditure (REE) and to explore the true 1 MET value for young Chinese adults with different body weight.A total of 251 young Chinese adults were divided into three groups: the normal weight group, the overweight group and the obese group. Their BEE, REE and 1 MET values were measured by Cortex Metamax 3B (MM3B). Multiple linear regressions and correlation analysis were used to examine factors that influence EE in Chinese population.The mean measured BEE and REE of young Chinese adults with normal weight, overweight and obesity was 1429, 1609, 1778 kcal/day and 1522, 1712, 1885 kcal/day, respectively. The EE per kilogram body weight decreased with the increasing body weight. The mean oxygen consumption in the three group was 3.78, 3.47, 3.21 mL/kg/min respectively. There was no significant difference in BEE and REE after adjustment for fat-free mass (FFM). The significant influencing factors were body mass index (BMI) and sex in BEE, FFM and BMI in REE in Chinese population.BEE and REE were significantly different for different body weights while these differences disappeared after adjustment for FFM, and people with different body weights may have different 1 MET values. Further studies should be conducted to obtain more accurate daily energy requirement and 1MET value for specific Chinese populations.

Background Iron nutrition is important for the health of women of reproductive age, and defining the physiologic requirement for iron can help them accurately plan the iron intake. However, research on the physiologic requirement for iron in women is insufficient worldwide. This study aimed to further improve the methodology and get more precise data for the physiological requirements for iron in women of reproductive age on the basis of our previous study. Method Sixty-one women of reproductive age who had not been pregnant before and during the whole study were included from Hebei province, China in 2015. Each subject participated in a 2-week metabolic trial with consuming 50 mg of the stable isotope 58Fe, and were then followed for ~ 800 days. The abundance of 58Fe and the total iron concentration in the circulation were measured using multi-collector inductively-coupled plasma mass spectrometry and atomic absorption spectroscopy. The physiologic requirement for iron in women of reproductive age was then calculated. Results The average iron circulation rate was 80.4%, and the steady period started from about 1 year. The average physiological requirement for iron of 21 subjects obtained by formula calculation was 1.55 mg/d and 23.63 μg.kg− 1.d− 1 after adjustment for body mass, and that of 33 subjects obtained by linear regression was 1.29 mg/d, 20.98 μg.kg− 1.d− 1 after adjustment for body mass. The results by two methods showed no significant difference. The EAR and RNI calculated from this results was 11–13 mg/d and 15–18 mg/d, respectively, both of which were slightly lower than the recommended value in Chinese Dietary Reference Intake (2013). Conclusion The physiological requirements for iron in women of reproductive age were in accordance with other studies, while the EAR and RNI calculated from which were slightly lower than Chinese present recommended value. Trial registration ChiCTR, ChiCTR-OCH-14004302. Registered 14 February 2014, http://www.chictr.org.cn/enindex.aspx Electronic supplementary material The online version of this article (10.1186/s12986-019-0384-1) contains supplementary material, which is available to authorized users.

Summary Background Few studies have used nationally representative data to describe dietary trends and the related cardiometabolic mortality burden in China. Thus, we aimed to characterise the trends in disease-related dietary factors as well as their associated disease burden among Chinese adults from 1982 to 2012. Methods For this cross-sectional population-based study, we analysed a nationally representative sample of 204 802 adults aged 20 years or older, using data from the 1982, 1992, 2002, and 2010–12 China National Nutrition Surveys (CNNS). We did a comparative risk assessment, in which the effects of suboptimal intakes of 12 dietary factors, individually and collectively, on cardiometabolic mortality were estimated by calculating the population attributable fraction (PAF) to estimate the proportional reduction in cardiometabolic deaths that would occur if exposure to each dietary risk factor was reduced to an alternative optimal level. Findings The overall PAF of mortality from cardiovascular disease and type 2 diabetes that was associated with suboptimal dietary quality was 62·2% in 1982, 57·9% in 1992, 56·2% in 2002, and 51·0% in 2010–12, which accounted for 21·6% of total mortality in China in 1982, 16·6% in 1992, 17·6% in 2002, and 20·8% in 2010–12. The estimated number of cardiometabolic deaths associated with suboptimal dietary intakes was 1·07 million in 1982, 0·93 million in 1992, 1·18 million in 2002, and 1·51 million in 2010–12. Of all 12 dietary factors examined, high sodium intake (17·3%), low fruit consumption (11·5%), and low marine omega-3 fatty acids (9·7%) were associated with the largest numbers of estimated cardiometabolic deaths in 2010–12. Interpretation We observed an improvement in several dietary factors in China in the past few decades. However, current intakes of these dietary factors remain suboptimal. Poor diet quality is estimated to be associated with a substantial proportion of deaths from heart disease, stroke, and type 2 diabetes in China. Funding Ministry of Health, China, and Ministry of Science and Technology, China.

To evaluate thyroid function and iodine nutritional status for pregnant women from various regions of China by determining plasma levels of thyrotrophin( TSH), free triiodothyronine( FT3), and free thyroxine( FT4).Plasma samples were obtained from pregnant women that participated in the 2010-2012 China National Nutrition and Health Survey. Plasma levels of TSH, FT3, and FT4 were determined by using an automated chemiluminescence immunoassay analyzer. Thyroid function was evaluated according to the determined levels.A total of 877 plasma samples collected from pregnant women residing in different regions of China wereanalyzed for levels of TSH, FT3, and FT4. The median TSH levels detected in the first, second, and third trimesters of pregnancy were 1. 56, 1. 84, and 1. 89 m IU/L, respectively. The median FT4 levels were 17. 21, 16. 29 and 16. 35 pmol/L, respectively. The median FT3 levels were 4. 70, 4. 39 and 4. 18 pmol/L, respectively. The rate of normal thyroid function for this cohort was 78. 1%, and for the pregnant women that resided in large cities the rate was 71. 2%. The most common type of abnormal thyroid function that was detected was subclinical hypothyroidism. It had an overall incidence of10. 5%, and those residing in regions of rural poverty had the highest rate( 14. 5%). The normal rate of thyroid function for adult pregnant women at the end of the trimester( 28-40 weeks) during pregnancy was significantly lower than that of the pregnant women in the first and second trimester.The rate of normal thyroid function in pregnant women is low( less than 80%). Subclinical hypothyroidism is the main type of thyroid dysfunction during pregnancy. It should be continued to pay attention to the relationship between the high incidence of subclinical hypothyroidism and iodine nutritional status of pregnant women, especially subclinical hypothyroidism caused by insufficient iodine intake.

Serum copper is an insensitive but reliable biomarker reflecting the change of copper nutritional status in both depleted and replete populations. The current study aimed to establish the reference values of serum copper in school-age children and pregnant women in China and to explore the adequate range of serum copper for both these two categories of people. A multistage, stratified, random sampling combined with probability proportionate to regional size sampling method was employed. A total of 4019 subjects (2736 school-age children and 1283 pregnant women) were selected from China Nutrition and Health Survey 2010-2012 (CNHS 2010-2012). The concentration of serum copper was determined by sector field inductively coupled plasma mass spectrometry (SF-ICP-MS). The adequate range of serum copper was determined by the logistic sigmoid saturation curve of the median derivatives. The median concentration of serum copper was 1140.9 μg/L with a range of 746.7-1677.6 μg/L for school-age children and 1933.4 μg/L with a range of 947.4-3391.4 μg/L for pregnant women. The adequate range of serum copper was 905.7-1440.7 μg/L for school-age children and 1308.8-2537.8 μg/L for pregnant women. These parameters represent an essential prerequisite for the assessment of copper nutritional status, as well as nutrition interventions.

This study was conducted to examine the possible association between vitamin A status and overnutrition in Chinese urban children and adolescents. Weight, height and serum retinol were assessed in total 3457 children (7–9.9 years of age) and adolescents (10–17 years of age), using urban region data from the China National Nutrition and Health Survey 2010–2013 (CHNNS2010-2013) which is a nationally representative cross-sectional study. Prevalence of low serum concentration of retinol was 26.8% and 12.24% for overweight. Retinol inadequacy was significantly higher in children (32.13%) than in adolescents (24.48%). The average of retinol was significantly higher in overnutrified 42.32 μg/dL versus non-overnutrified 41.05 μg/dL (p = 0.00) children and adolescents. Overnutrified children and adolescents presented a greater chance of an increase in serum concentration of retinol (odds ratio 1.34, 95% confidence interval 1.10–1.63, and 1.48, 95% confidence interval (1.26–1.74) when compared with non-overnutrified children. An important correspondence between vitamin A deficiency and overnutrition was found. Non-overnutrified children and adolescents may have a greater chance of presenting low concentrations of retinol. Future public health strategies focused on the overnutrified population and vitamin A supplements should consider the effect of retinol on urban children and adolescents in China.

To establish a reliable method of iodine nutritional assessment for pregnant women using the adjusted urinary creatinine of spot urine and 24-hour urine samples.The spot urine in the morning at 8 00-11:30 and the following 24-hour urine samples of pregnant women were collected in Peking Union Medical College Hospital during March to October in 2016. The urinary iodine and urinary creatinine concentrations were determined. The estimated 24-hour urinary iodine excretion( 24-h UIE) was calculated using the urinary iodine/urinary creatinine( UIC/Cr) ratio of spot urine, and 24-hour urinary creatinine excretion( 24-h UCr E).There was a significant difference in the UIC, UIC/Cr ratio between spot urine and 24-h urine( P0. 05). The UIC, UIC/Cr ratio of spot urine, and the estimated 24-h UIE all were significantly correlated with the real 24-h UIE( P0. 01), especially the estimated 24-h UIE were taking up 70%-80% for the real 24-h UIE in the period of gestation.The UIC, UIC/Cr, and the estimated 24-h UIE cannot directly replace the relevant values derived of the 24-hour urine samples. But the estimated 24-h UIE can reflect the iodine nutritional status of pregnant women in recent days, and the method could be a reliable reference for the iodine nutritional assessment of the pregnant women in practice.

To investigate the difference between three methods for estimating the plasma volume in children.The blood volume of 57 children from 10 to 12 years old were estimated by weight, formula proposed by Woodhead, and by bioelectrical impedance analysis( BIA), the effect and consistency of three methods were also compared.There was no difference between three methods in blood volume estimating( P0. 05). According to the results of PassingBablok regression regression analysis, the residual standard deviation( RSD) was 85. 88, 122. 83 and 111. 24. The regression equation were: y_(Woodhead)= 577. 356224 + 0. 800525x_(BW); y_(BIA)=1091. 590338 + 0. 653032x( BW); y( BIA)= 560. 545257 + 0. 843899x_(Woodhead), and there was no obvious departure from the linear correlation( P0. 05). After regrouping by the percent body fat, there were differences between three methods in weight in overweight and obese children group( P0. 05), the blood volume estimated by weight was thehighest, and by the BIA was the lowest. While in normal group there was no difference between three groups(P0. 05).Three methods in blood volume estimating has a good consistency, estimating by body weight and formula have the virtue of convenience. Method of BIA is extremely fit for application in children with higher body fat.

To explore the relationship of diabetes in older Han adults with leptin receptor gene rs1137100 and rs1137101 single nucleotide polymorphism( SNP).A total of 511 older Han adults were selected for this study and divided into diabetes and control groups, with 241 and 270 people in each group. The genotypes of rs1137100 and rs1137101 and the allele frequency were determined by Taqman method. The correlations between genotypes of leptin receptor gene SNPs with diabetes wereanalyzed. Blood concentrations of lipids, fasting serum glucose( FBG), leptin and insulin were also measured.The distribution of GG, GA and AA genotypes in rs1137100 in the diabetes group and the healthy group was 73. 0%, 24. 5%, 2. 5% and 68. 9%, 28. 9%, 2. 2%, respectively. The difference was not significant between the two groups( χ~2= 1. 27, P = 0. 53). There was no significance in the difference of the frequency of "A"allele in rs1137100 in the two groups, with 14. 7% and 16. 7% in each group( χ~2= 0. 72, P = 0. 40). In rs1137101, the distribution of GG, GA and AA genotypes in the diabetes group and the healthy group was 77. 6%, 21. 2%, 1. 2% and 77. 8%, 21. 1%, 1. 1%, respectively. The difference was not significant between the two groups( χ~2= 0. 02, P = 0. 99). There was no significance in the difference of the frequency of"A"allele in rs1137101 in the two groups, with 11. 8% 11. 7% in each group( χ~2=0. 01, P = 0. 94. The Logistic analysis showed that GA and AA genotypes carriers in rs1137100 had similar risk for diabetes when comparing with the GG genotypes( OR =1. 06, 95% CI 0. 34-3. 34, OR = 0. 80, 95% CI 0. 54-1. 19). The GA and AA genotypes carriers in rs1137101 had similar risk for diabetes when comparing with the GG genotypes, and the OR values were 1. 12( 95% CI 0. 22-5. 63) and 1. 01( 95% CI0. 66-1. 54), respectively.The rs1137100 and rs1137101 polymorphism are not significantly associated with the prevalence of diabetes in older Han adults.

To assess the vitamin A status of Chinese rural elderly population by analyzing the serum retinol levels of Chinese rural elderly residents in 2010-2012.Data were from the Chinese National Nutrition and Health Survey in2010-2012. Using the multistage stratified cluster sampling method, serum samples from the elderly residents aged above 60 years old( including), were obtained from 45 general rural and 30 poor rural. The basic situation of the survey object was collected by the questionnaire survey. The concentration of serum retinol was determined by high performance liquid chromatography( HPLC). The vitamin A deficiency( VAD) was judged by the related standard recommended by WHO. A total of 2 413 elderly residents were included in the study. The levels of serum retinol and the prevalence of vitamin A deficiency and marginal vitamin A deficiency were also compared.The level of serum retinol for rural elderly residents was 1. 88( 1. 45-2. 45) μmol/L. The serum retinol level of poor rural resident was( 1. 92( 1. 46-2. 48) μmol/L), which was significant for the ordinary rural residents( 1. 86( 1. 44-2. 41) μmol/L)( χ~2= 5. 1428, P = 0. 0233). The serum retinol of male elderly( 1. 97( 1. 48-2. 54)) was statistically higher than female( 1. 82( 1. 42-2. 33))( χ~2= 22. 3383, P0. 0001). The prevalence of VAD among Chinese rural elderly residents was 2. 28%, 2. 79% for ordinary rural residents and 1. 53% for poor rural residents. The marginal VAD rate of Chinese rural elderly residents was 6. 30%, 6. 84% for ordinary rural residents and 5. 51% for poor rural residents. The prevalence of VAD and marginal VAD for male was 2. 18% and5. 57%, respectively. The prevalence of VAD and marginal VAD for female was 2. 38%and 7. 21%, respectively. No statistically difference was observed between different genders. The prevalence of VAD was 1. 93%, 3. 17% and 1. 47% for different age groups( 60 ~ 69, 70 ~ 79 and above 80 years old), and no statistically difference was observed between different age groups. The prevalence of marginal VAD was 5. 87%, 6. 20% and 11. 74% for different age groups, and statistically difference was observed between different age groups( χ~2= 7. 3858, P = 0. 039).Chinese rural elderly population has a certain degree of vitamin A deficiency and marginal deficiency, the marginal deficiency of the elderly over 80 years old is more common.

To analysis the vitamin A nutritional status of 6-to 17-year-old children and adolescents between 2010-2012 in rural China.Based on the China Health and Nutrition Survey 2010-2012 project, the serum vitamin A samples of children and adolescents from survey sites of rural area were selected by random sampling method, and 5888 cases of samples were detected using the high performance liquid chromatography method to evaluate their vitamin A nutritional status.The serum retinol concentration of children and adolescents in rural area was( 1. 44 ± 0. 58) μmol/L, and were( 1. 40 ± 0. 56) μmol/L in general rural areas and( 1. 46 ± 0. 60) μmol/L in poverty rural areas respectively( t = 1. 88, P0. 05). The serum retinol concentration of6-to 11-year-old were( 1. 31 ± 0. 53) μmol/L and( 1. 37 ± 0. 60) μmol/L, and serumretinol concentration of 12-to 17-year-old were( 1. 52 ± 0. 57) μmol/L and( 1. 52 ±0. 60) μmol/L( t = 11. 90 and t = 5. 54, P0. 05). The prevalence of vitamin A deficiency was 5. 53%, and were 6. 14% in general rural areas and 4. 55% in poverty rural areas, respectively( P0. 05). The prevalence of marginal vitamin A deficiency were 18. 75%, and were 19. 17% in general rural areas and 18. 07% in poverty rural areas, respectively( P0. 05).The situation of vitamin A deficiency in rural China are still widespread, the high prevalence of marginal vitamin A deficiency is more prominent.

To evaluate nutritional status of vitamin A( VA) among Chinese rural fertile women in 2010-2012.A multi-stage stratified sampling and population proportional stratified random sampling method was used and the participants of rural fertile women who were 18-44 years old and had completed personal information data during the 2010-2012 National Nutrition and Health Survey were included( n = 1279). The level of serum retinol, rate of VA marginal deficiency anddeficiency among the fertile women were analyzed.In 2010-2012, the P50( P25-P75) level of serum retinol was 1. 63( 1. 28-2. 10) μmol/L for Chinese rural fertile women, 1. 60( 1. 25-2. 05) μmol/L for fertile women from ordinary rural area and1. 70( 1. 32-2. 17) μmol/L for fertile women from poverty rural area. The level of serum retinol of fertile women from poverty rural area was statistically higher than that of ordinary rural area( P = 0. 007). The rate of VA marginal deficiency and deficiency among the Chinese rural fertile women was 9. 46% and 3. 05%, respectively. The rate of VA marginal deficiency and deficiency among the fertile women from ordinary rural area and poverty rural area was 9. 82%, 4. 13%, 9. 03% and 1. 74%, respectively. The rate of VA deficiency among the fertile women from ordinary rural was statistically higher than that of poverty rural( P = 0. 013), but no statistically differences in the rate of VA marginal deficiency were found( P0. 05). Moreover, the statistically differences of rate of VA marginal deficiency and deficiency were also observed in the different regions and educational level( only VA deficiency). There were no statistically differences of VA marginal deficiency and deficiency were found in the other variables.The different extent of VA deficiency still present and more popular for the VA marginal deficiency among Chinese fertile women.

To perform zinc nutritional assessment of children aged 2- 5years in rural areas of China according to the joint recommendation by the WHO /UNICEF / IAEA / IZi NCG.Based on the project of China Nutrition and Health Survey 2002( CNHS 2002), a stratified sampling combined with the probability proportional to population size sampling method was employed. The concentration of plasma zinc was determined by high resolution inductively coupled plasm mass spectrometry, and the data of dietary zinc intake and the physical examination was collected. The assessment of zinc nutrition was obtained by 3 aspects of indicators involving plasma zinc, dietary zinc intake and the development of growth.Plasma zinc concentration was 714. 9 μg / L, and 45. 5% of children with zinc deficiency. Dietary zinc intake was 5. 9 mg / d, and the prevalence of inadequate dietary zinc intake was 18. 1%. The height for age Z score was- 0. 8, with 18. 2% of children who were stunted.Zinc nutritional status of children aged 2- 5 years in rural areas is poor in the CNHS 2002. The status of plasma zinc is utmost serious in comparison withother indicators. The status of zinc nutrition in children aged 2- 5 years from the 4th rural areas is required to be continuous attention. Zinc nutrional assessment provides an extremely useful information and technical reference for early childhood.

To obtain the serum 25-hydroxyvitamin D status for women of child-bearing age aged 18-44 years from rural areas in China Health and Nutrition Survey2010-2012( CHNS 2010-2012).We randomly selected the 1520 representative women of childbearing age from the 75 monitoring spots derived in the CNHS 2010-2012. The information of study population were collected using questionnaires and the serum 25-hydroxyvitamin D concentration was determinedusing the RIA kits. The vitamin D nutritional status was classified by the standards suggested by the America Endocrine Society. Multiple Logistic regression analysis was explored therelationship between vitamin D status with the potential relevant factors.The median serum 25-hydroxyvitamin D concentration was 22. 7 ng/m L ranged from 8. 2 to42. 0 ng/m L. The prevalence of vitamin D deficiency was 38. 7%, insufficiently 39. 5%and sufficiently 21. 8%. Multiple Logistic regression analysis showed that nationality, geographical regions and income per capita was the significant.s The nutritional status of vitamin D in women of child bearing-age is not better in the CNHS2010-2012. We suggest that it should be pay more attention on the women of childbearing age for the minority, north China, family income per year between 10000 and 30000 Yuan.

This study investigated the composition and proportions of fecal microbiota in Sprague-Dawley rats after consuming two genetically modified (GM) corn lines in comparison with the isogenic corn and the AIN93G standard feed for 10 weeks using bar-coded 16S rRNA gene sequencing. As a result, GM corn did not significantly alter the overall health and alpha-diversity of fecal microbiota. Fecal microbiota structures could be separated into noncorn and corn but not non-GM and GM corn subgroups. Both non-GM and GM corn caused the increase in bacterial populations related to carbohydrates utilization, such as Lactobacillus, Barnesiella, and Bifidobacterium, and the reduction in potentially pathogenic populations, such as Tannerella and Moraxellaceae. In conclusion, similar effects on the fecal microbiota were observed after consuming a GM- and non-GM-corn-based diet for long periods. Further studies are warranted to elucidate the functional relevance of the changes in the proportions of bacterial populations in these diets.

ObjectiveTo evaluate vitamin D deficiency prevalence and risk factors among pregnant Chinese women.DesignA descriptive cross-sectional analysis.SettingChina National Nutrition and Health Survey (CNNHS) 2010–2013.SubjectsA total of 1985 healthy pregnant women participated. Possible predictors of vitamin D deficiency were evaluated via multiple logistic regression analyses.ResultsThe median serum 25-hydroxyvitamin D level was 15·5 (interquartile range 11·9–20·0, range 3·0–51·5) ng/ml, with 74·9 (95 % CI 73·0, 76·7) % of participants being vitamin D deficient (25-hydroxyvitamin D P=0·016), lack of vitamin D supplement use (P=0·021) and low ambient UVB level (PP=0·012) and low ambient UVB level (PP=0·050), later gestational age (P=0·035), higher pre-pregnancy BMI (P=0·019), low ambient UVB level (PP=0·007).ConclusionsVitamin D deficiency is prevalent among pregnant Chinese women. Residing in areas with low ambient UVB levels increases the risk of vitamin D deficiency, especially for women experiencing advanced stages of gestation, for younger pregnant women and for women of Hui ethnicity; therefore, vitamin D supplementation and sensible sun exposure should be encouraged, especially in the winter months. Further studies must determine optimal vitamin D intake and sun exposure levels for maintaining sufficient vitamin D levels in pregnant Chinese women.

Background We compared the utility of two 25-OH-D immunoassays to a liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for assessing human serum samples from the Chinese population. Methods In total, 451 Chinese serum samples were tested using the IDS 25-Hydroxy Vitamin D EIA kits and the LC–MS/MS 25-OH-D method. Of these, 239 samples were also analysed using DiaSorin 25-Hydroxyvitamin D RIA kits. Results The regression coefficients (r) between LC–MS/MS and immunoassays were 0.8430 (95% CI: 0.8140–0.8678) for IDS-EIA and 0.8187 (95% CI: 0.7720–0.8566) for DiaSorin-RIA. Using the Passing–Bablok analysis, IDS-EIA and DiaSorin-RIA yielded results equivalent to those from LC–MS/MS. However, IDS-EIA showed negative bias and DiaSorin-RIA showed positive bias when compared to LC–MS/MS, as indicated by the Bland–Altman plots. By the kappa test, both IDS-EIA and DiaSorin-RIA were in acceptable agreement with LC–MS/MS in the assessment of vitamin D deficiency and insufficiency. Conclusion Although some systematic bias was found, the DiaSorin-RIA and IDS-EIA kits met the minimum performance goal for 25-OH-D measurement and can be recommended for routine use in the Chinese population. However, further studies are needed to assess 25-OH-D measurement immunoassays with lower bias and that are more suitable for the Chinese population.

NaFeEDTA has been applied in many foods as an iron fortificant and is used to prevent iron deficiency in Fe-depleted populations. In China, soy sauce is fortified with NaFeEDTA to control iron deficiency. However, it is unclear whether Fe-fortified soy sauce affects zinc absorption. To investigate whether NaFeEDTA-fortified soy sauce affects zinc absorption in children, sixty children were enrolled in this study and randomly assigned to three groups (10 male children and 10 female children in each group). All children received daily 3 mg of (67)Zn and 1.2 mg of dysprosium orally, while the children in the three groups were supplemented with NaFeEDTA-fortified soy sauce (6 mg Fe, NaFeEDTA group), FeSO₄-fortified soy sauce (6 mg Fe, FeSO₄ group), and no iron-fortified soy sauce (control group), respectively. Fecal samples were collected during the experimental period and analyzed for the Zn content, (67)Zn isotope ratio and dysprosium content. The Fe intake from NaFeEDTA-fortified and FeSO₄-fortified groups was significantly higher than that in the control group (P < 0.0001). The daily total Zn intake was not significantly different among the three groups. There were no significant differences in fractional Zn absorption (FZA) (P = 0.3895), dysprosium recovery (P = 0.7498) and Zn absorption (P = 0.5940) among the three groups. Therefore, NaFeEDTA-fortified soy sauce does not affect Zn bioavailability in children.

Vitamin D plays a critical role in calcium and phosphate metabolism and helps maintain skeletal integrity in childhood, yet vitamin D status in Chinese children and adolescents is not well documented. The aim of this study was to assess the vitamin D status and analyze the risk factors for vitamin D deficiency in Chinese children and adolescents aged 6–17 years. Serum 25 hydroxyvitamin D (25(OH)D) was measured with a radioimmunoassay kit in 15,000 children and adolescent participants in the Chinese national nutrition and health survey (CNNHS) 2010–2012. Age, gender, region type, ethnicity, outdoor time, and vitamin D supplementation were recorded in unified design questionnaires. The season was recorded by the date of blood taken; location was divided into north and south by China′s Qinling Mountains and Huaihe River; and ambient ultraviolet B (UVB) radiation level was classified according to the corresponding dose of each participant living area from National Aeronautics and Space Administration data. 14,473 participants from the cross-sectional study of CNNHS 2010–2012 were included in this study. The median serum 25(OH)D concentration was 48.2 (35.4–63.4) nmol/L, and the concentration for males was 50.0 (36.5–65.7) nmol/L, which was statistically higher than that of females (46.7 (34.4–60.9) nmol/L) (P < 0.001). The general prevalence of vitamin D deficiency was 53.2%; 50.0% for males and 56.5% for females at the cut-off 50 nmol/L. According to the results of the log-binomial regression analysis, vitamin D deficiency in Chinese children and adolescents was specifically related to female gender (P < 0.0001), to ages 12–14 years (P < 0.0001) and 15–17 years (P < 0.0001), living in large cities (P < 0.0001) or ordinary rural areas (P < 0.0001), low ambient UVB levels (P < 0.0001) and medium ambient UVB levels (P < 0.0001), spring (P < 0.0001), autumn (P < 0.0001) and winter seasons (P < 0.0001). The data showed that vitamin D deficiency was very common among children and adolescents aged 6–17 years in China. Effective sun exposure should be encouraged in both genders aged 6–17 years, dietary vitamin D and vitamin D supplements are also recommended, especially in the seasons of spring and winter.

Background Vitamin D inadequacy is common among the elderly, especially within the Asian population. The vitamin D status among healthy adults in the elderly Chinese population was evaluated. Methods A total of 6014 healthy adults aged 60 years or older (2948 men, 3066 women) participated in this descriptive cross-sectional analysis. Possible predictors of vitamin D inadequacy were evaluated via multiple logistic regression analyses. Results The median serum 25-hydroxyvitamin D (25(OH)D) levels were 61.0 nmol/l (interquartile range (IQR) 44.3–80.6, range 5.1–154.5) for men and 53.7 nmol/l (IQR 38.8–71.0, range 6.0–190.0) for women, with 34.1% (95% confidence interval (CI) 32.4–35.8) of men and 44.0% (95% CI 42.2–45.8) of women presenting vitamin D inadequacy (25(OH)D

The development of reference values of trace elements is recognized as a fundamental prerequisite for the assessment of trace element nutritional status and health risks. In this study, a total of 1400 pregnant women aged 27.0 ± 4.5 years were randomly selected from the China Nutrition and Health Survey 2010-2012 (CNHS 2010-2012). The concentrations of 14 serum trace elements were determined by high-resolution inductively coupled plasma mass spectrometry. Reference values were calculated covering the central 95% reference intervals (P2.5-P97.5) after excluding outliers by Dixon's test. The overall reference values of serum trace elements were 131.5 (55.8-265.0 μg/dL for iron (Fe), 195.5 (107.0-362.4) μg/dL for copper (Cu), 74.0 (51.8-111.3) μg/dL for zinc (Zn), 22.3 (14.0-62.0) μg/dL for rubidium (Rb), 72.2 (39.9-111.6) μg/L for selenium (Se), 45.9 (23.8-104.3) μg/L for strontium (Sr), 1.8 (1.2-3.6) μg/L for molybdenum (Mo), 2.4 (1.2-8.4) μg/L for manganese (Mn), 1.9 (0.6-9.0) ng/L for lead (Pb), 1.1 (0.3-5.6) ng/L for arsenic (As), 835.6 (219.8-4287.7) ng/L for chromium (Cr), 337.9 (57.0-1130.0) ng/L for cobalt (Co), 193.2 (23.6-2323.1) ng/L for vanadium (V), and 133.7 (72.1-595.1) ng/L for cadmium (Cd). Furthermore, some significant differences in serum trace element reference values were observed between different groupings of age intervals, residences, anthropometric status, and duration of pregnancy. We found that serum Fe, Zn, and Se concentrations significantly decreased, whereas serum Cu, Sr, and Co concentrations elevated progressively compared with reference values of 14 serum trace elements in pregnant Chinese women. The reference values of serum trace elements established could play a key role in the following nutritional status and health risk assessment.

In this study, we assessed the hemoglobin levels and anemia status of Chinese children and adolescents from the Chinese National Nutrition and Health Survey (CNNHS) in 2010–2012 and analyzed the factors associated with anemia. The hemoglobin concentration and prevalence of anemia for children and adolescents aged 6–17 years from both CNNHS 2010–2012 and CNNHS 2002 were analyzed. Multi-variable logistic regression analysis was used to assess the factors associated with anemia. The mean hemoglobin concentration increased among Chinese children and adolescents, from 135.2 ± 13.9g/L in CNNHS 2002 to 141.2 ± 15.8 g/L in CNNHS 2010–2012, with the prevalence of anemia decreasing from 12.6% to 6.6% (p&lt, 0.0001). Anemia was specifically related to girls (p &lt, 0.0001), children aged 6–8 years (p = 0.0175), 12–14 years (p = 0.0007), and 15–17 years (p &lt, ordinary rural areas (p = 0.0009) and poor rural areas (p &lt, spring (p &lt, 0.0001), autumn (p &lt, 0.0001), and winter (p &lt, underweight individuals (p &lt, and an annual average income per capita of less than 20,000 RMB (p &lt, 0.0001).The prevalence of anemia in Chinese children and adolescents has improved significantly in comparison to 10 years prior, however, it remains a public health problem in this population. Further research is required to understand the determinants of iron status, which could then lead to strategies to alleviate iron deficiency for Chinese children and adolescents, especially for girls, those living in rural areas, underweight individuals, and those with a low family income.

Anemia is a public health issue for developing countries, especially for women of childbearing age. The aim of this study was to assess the anemia status and analyze the risk factors for anemia in Chinese childbearing women aged 18&ndash, 49 years. Hemoglobin concentration was measured by the HiCN method in the Fifth Chinese National Nutrition and Health Survey (CNNHS) in 2010&ndash, 2012. Age, region type, ethnicity, bodyweight, height, education, season and smoke habit were recorded in unified questionnaires. Latitude was divided by China&rsquo, s Qinling Mountains and Huaihe River. Childbearing women (28,289) from the CNNHS 2010&ndash, 2012 were included in this study. The median hemoglobin concentration was 136.2(126.6&ndash, 145.0) g/L, and it was significantly higher than in CNNHS 2002 (132.5 (122.3&ndash, 141.6) g/L). The prevalence of anemia was 15.0%, and it was significantly lower than 10 years ago. The logistic regression analysis showed anemia in Chinese childbearing women was specifically related to 30&ndash, 39 age group (P = 0.004), in spring (P &lt, 0.0001) or in winter (P = 0.006), small and medium-sized cities (P = 0.044) and middle school education level (P = 0.027). The results showed that anemia status among childbearing women was greatly improved over 10 years since 2002, but it was still more severe than the rest of the populations. The nutrition propaganda and education is recommended for childbearing women to help them to improve the nutritional status on their own.

To investigate the relationship of leptin receptor gene rs1137100 and rs1137101 single nucleotide polymorphrism (SNP) with metabolic syndrome (MS) in older Han adults from major cities in China.A total of 2082 older Han adults were selected from 18 major cities including 15 provinces/municipalities of China National Nutrition and Health Survey in 2002. According to the MS definition proposed by Joint Interim Statement (JIS), the subjects were divided into MS and control groups. Plasma leptin and insulin levels were measured. The genotypes of rs1137100 and rs1137101 were detected by Taqman method. Association of genotypes of leptin receptor gene SNPs with MS was investigated.The MS group showed higher body mass index (BMI), waist circumference, fasting serum glucose, systolic blood pressure (SBP) and diastolic blood pressure (DBP), triglycerides (TG), serum total cholesterol (TC), insulin, homeostasis model of assessment for insulin resistence index (HOMA-IR) and leptin levels than those of control individuals, while the high density lipoprotein cholesterol (HDL-c) was significantly lower than the control group. The, GG, AA, GA genotypes distribution and the A allele frequency of rs1137100 and rs1137101 were similar between the two groups. The DBP and SBP level were obviously higher in AA genotype. The HDL-c concentration Was significantly lower in AA and GA + AA genotype. The AA and GA genotypes carriers in rs1137100 had similar risk for MS when comparing with the GG genotypes, and the OR values were 1.23 (95% CI 0.90-1.67) and 2.23 (95% CI 0.83-6.44), respectively. The AA and GA genotypes carriers in rs1137101 had similar risk for MS when comparing with the GG genotypes, and the OR values were 1.23 (95% CI 0.90-1.67) and 2.23 (95% CI 0.83-6.44), respectively.Leptin receptor genes rs1137100 and rs1137101 are not associated with pathogenesis of MS in older Han adults, but it may relate with hypertension or lipid abnormality.

To assess the distribution of serum ferritin, serum soluble transferrin receptor and body iron among girls and women by age and anaemia.Serum ferritin, serum soluble transferrin receptor and high sensitive C-reactive protein of 1625 and 1372 women in general and anaemic were measured in the National Health and Nutrition Survey commenced in 2010.The distributions of serum ferritin, serum soluble transferrin receptor and body iron for 6-11-y, 12-17-y, 18-44-y, 45-59-y and=60-y subgroups were significantly different. Both in population-representative women and those anaemic, the iron status of 18-44-y women was the lowest and that of 12-17-y girls the second lowest. The iron status of anaemic women was lower than that in representative women at ages 12-17 y, 18-44 y, 45-59 y and=60 y.Iron status in women of different ages and anaemic had different distributions, but consistently lower than that of population-representative women. The observed iron status of Chinese urban women supports program planning for iron nutrition promotion in women. Iron status information is also needed for men and to understand the pathogenesis which may be related to intake or loss.背景与目的：评估不同年龄和贫血状况女孩和成年女性的血清铁蛋白、血清转 铁蛋白受体和铁储量的分布情况。方法与研究设计：2010 年中国居民营养与健 康状况监测中，对1625 名普通女性和1372 名贫血女性分别测定其血清铁蛋 白、血清转铁蛋白受体和高敏C 反应蛋白浓度。结果：6-11、12-17、18-44、 45-59 和=60 岁不同年龄组女性之间血清铁蛋白、血清转铁蛋白受体和铁储量的 分布显著不同。在普通女性组和贫血女性组中，18-44 岁年龄组的女性铁营养状 况均为最差，其次为12-17 岁年龄组。在12-17、18-44、45-59 和=60 岁年龄组 中，贫血女性的铁营养状况显著低于普通女性组。结论：不同年龄组及贫血状 况女性的铁营养状况分布不同，贫血组女性的铁营养状况较普通女性组差。本 研究观察到的中国大城市女性铁营养状况的数据，支持开展针对女性的铁营养 改善的项目规划，也需要调查男性铁营养状况，并探究可能与铁摄入或损失的 发病机制。.

The level of blood hemoglobin and the anemia status of Chinese urban residents in 2010-2012 was analyzed.All the data in this study came from the China Nutrition and Health Survey in 2010-2012. By using multi-stage stratified sampling and population proportional stratified random sampling method, 74 276 residents aged above 6 from 34 metropolis and 41 middle-sized and small cities were included in this study. The concentration of blood hemoglobin was determined by cyanmethemoglobin method. Anemia was judged by the anemia standard recommended by WHO, combined with elevation correction standard. The level of blood hemoglobin, the prevalence of anemia and the 95%CI value were analyzed by using the complex sampling weighted processing, combined with the population figures released by the National Bureau of Statistics in 2009.In 2010-2012, the level of blood hemoglobin of Chinese city population was(144.16 ± 0.78)g/L, (152.88 ± 0.94)g/L for male and(135.01 ± 0.71)g/L for female, while (145.65 ± 1.22)g/L for metropolis and (143.90 ± 0.89)g/L for small and medium-sized. The anemia prevalence of Chinese city population (pregnant women were not included) was 9.7%(95%CI: 9.4%-10.1%), 6.8%(95%CI: 6.4%-7.3%) for male and 12.8%(95%CI: 12.2%-13.4%) for female, while 8.5%(95% CI: 8.0%-9.0%) for metropolis and 10.0%(95%CI: 9.5%-10.4%) for small and medium-sized. The anemia prevalence of 18-44 women (15.4%, 95%CI: 14.3%-16.6%) was the highest among all the age-groups, and the average anemia prevalence of people more than 60 years-old (including) (12.5%, 95%CI: 11.8%-13.2%) was higher than the other age-groups.The anemia prevalence of Chinese city population in 2010-2012 was obviously decreased in comparison of 10 years ago, while, more attention and improvement measures should be take upon women at reproductive age and the elder people.

To quantitatively assess the concentration and the distribution of the body iron (BI) for women in the poor rural areas.Resampling based on the blood sample of Nutrition and Health Survey in China from 2010 to 2012, serum ferritin (SF) and serum soluble transferrin receptor (sTfR) were measured for the selected blood samples by immunoturbidimetric assay. BI was calculated, and the concentrations and distribution in different aged subgroup were assessed. Distribution of BI subgrouped in different aged women was compared. The correlations of BI to hemoglobin (Hb), SF and sTfR were analyzed.The average levels of BI were (7.35 ± 2.37) mg/kg for schoolgirls aged 6-11 years, (6.50 ± 3.59) mg/kg for adolescent girls aged 12-17 years, (6.07 ± 4.78) mg/kg for women of childbearing aged 18-44 years, (8.60 ± 4.45) mg/kg for women aged 45-59 years, and (10.35 ± 3.38) mg/kg for elderly female aged ≥ 60 years, respectively. The proportion of low BI in the 12-17 years and 18-44 years women was the highest. BI level was positively correlated to SF and negatively correlated to sTfR (P0.01). BI level was positively correlated to Hb in the BI0 mg/kg subgroup.The BI methodology allows the full range of iron status of populations to be evaluated. The iron status for adolesecent girls aged 12-17 years and women of childbearing aged 18-44 years should be improved.