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Your search keyword '"Jiang, Zhengning"' showing total 24 results
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24 results on '"Jiang, Zhengning"'

2. Map-based cloning of the gene Pm21 that confers broad spectrum resistance to wheat powdery mildew

Author

He, Huagang, Zhu, Shanying, Ji, Yaoyong, Jiang, Zhengning, Zhao, Renhui, and Bie, Tongde

Subjects
Quantitative Biology - Quantitative Methods
Abstract

Common wheat (Triticum aestivum L.) is one of the most important cereal crops. Wheat powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is a continuing threat to wheat production. The Pm21 gene, originating from Dasypyrum villosum, confers high resistance to all known Bgt races and has been widely applied in wheat breeding in China. In this research, we identify Pm21 as a typical coiled-coil, nucleotide-binding site, leucine-rich repeat gene by an integrated strategy of resistance gene analog (RGA)-based cloning via comparative genomics, physical and genetic mapping, BSMV-induced gene silencing (BSMV-VIGS), large-scale mutagenesis and genetic transformation.

Published
2017

5. TaCHP encoding C1‐domain protein stably enhances wheat yield in saline‐alkaline fields.

Author

Xiao, Guilian, Wang, Minqin, Li, Xiaomeng, Jiang, Zhengning, Zhang, Hongjian, Gao, Derong, Zhang, Boqiao, Xia, Guangmin, and Wang, Mengcheng

Subjects
WINTER wheat, CORN, REVERSE transcriptase polymerase chain reaction
Abstract

A study published in the Journal of Integrative Plant Biology explores the potential of the zinc finger gene TaCHP to enhance wheat yield in saline-alkaline conditions. The researchers conducted multi-year, multi-site field trials and found that overexpressing TaCHP significantly increased wheat yields in saline-alkaline fields without affecting yields under normal conditions. Genetic variations in the promoter of TaCHP were also identified, with one haplotype (Hap1) conferring greater salt tolerance than the other (Hap2). The study suggests that TaCHP and its tolerant haplotype have promising applications in molecular breeding for stress-tolerant wheat. [Extracted from the article]

Published
2024
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13. Genome-wide association mapping revealed syntenic loci QFhb-4AL and QFhb-5DL for Fusarium head blight resistance in common wheat (Triticum aestivum L.)

Author

Author), Wenjing Hu(Former Corresponding, primary, Gao, Derong, additional, Wu, Hongya, additional, Liu, Jian, additional, Zhang, Chunmei, additional, Wang, Junchan, additional, Jiang, Zhengning, additional, Liu, Yeyu, additional, Li, Dongsheng, additional, Zhang, Yong, additional, and Author), Chenbin Lu(New Corresponding, additional

Published
2019
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14. Development of a kompetitive allele-specific PCR marker for selection of the mutatedWx-D1dallele in wheat breeding

Author

Shunhe Cheng, Zhang Yong, Jiang Zhengning, Xin Yi, Datong Liu, Ronglin Wu, Tongde Bie, Hu Wenjing, Xiao-Ming Cheng, Derong Gao, and Yun Zhao

Subjects
0106 biological sciences, 0301 basic medicine, Genetics, Sequence analysis, Point mutation, food and beverages, Locus (genetics), Single-nucleotide polymorphism, Plant Science, Biology, 01 natural sciences, Molecular biology, Stop codon, Endosperm, 03 medical and health sciences, 030104 developmental biology, Allele, Variants of PCR, Agronomy and Crop Science, 010606 plant biology & botany
Abstract

Waxy (Wx) protein is a key enzyme for synthesis of amylose in endosperm. Amylose content in wheat grain influences the quality of end-use products. Seven alleles have been described at the Wx-D1 locus, but only two of them (Wx-D1b, Wx-D1e) were genotyped with codominant markers. The waxy wheat line K107Wx1 developed by treating ‘Kanto 107’ seeds with ethyl methanesulphonate carries the Wx-D1d allele. However, no molecular basis supports this nomenclature. In the present study, DNA sequence analysis confirmed that a single nucleotide polymorphism in the sixth exon of Wx-D1 changed tryptophan at position 301 into a termination codon. Based on this sequence variation, a PCR-based KASP marker was developed to detect this point mutation using 68 BC8F1 plants and 297 BC8F2 lines derived from the cross ‘Ningmai 14’*9/K107Wx1. Combined with codominant markers for the Wx-A1 and Wx-B1 alleles, waxy and non-waxy near-isogenic lines were distinguished. The KASP marker was efficient in identifying the mutant allele and can be used to transfer waxiness to elite lines.

Published
2017

17. Genetic Analysis of Fusarium Head Blight Resistance in CIMMYT Bread Wheat Line C615 Using Traditional and Conditional QTL Mapping

Author

Yi, Xin, primary, Cheng, Jingye, additional, Jiang, Zhengning, additional, Hu, Wenjing, additional, Bie, Tongde, additional, Gao, Derong, additional, Li, Dongsheng, additional, Wu, Ronglin, additional, Li, Yuling, additional, Chen, Shulin, additional, Cheng, Xiaoming, additional, Liu, Jian, additional, Zhang, Yong, additional, and Cheng, Shunhe, additional

Published
2018
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24. The Hv-SGT1 Gene from Haynaldia villosa Contributes to Resistances Towards Both Biotrophic and Hemi-Biotrophic Pathogens in Common Wheat (Triticum aestivum L.).

Author

Xing, Liping, Qian, Chen, Cao, Aizhong, Li, Yingbo, Jiang, Zhengning, Li, Minghao, Jin, Xiahong, Hu, Jiameng, Zhang, Yiping, Wang, Xiue, and Chen, Peidu

Subjects
WHEAT, HAYNALDIA, PROTEINS, PLANT species, CYTOPLASM, GENE expression in plants, JASMONATE
Abstract

The SGT1 protein is essential for R protein-mediated and PAMPs-triggered resistance in many plant species. Here we reported the isolation and characterization of the Hv-SGT1 gene from Haynaldia villosa (2n = 14, VV). Analysis of the subcellular location of Hv-SGT1 by transient expression of a fusion to GFP indicated its presence in the cytoplasm and nucleus. Levels of Hv-SGT1 transcripts were increased by inoculation with either the biotrophic pathogen Blumeria graminis DC. f. Sp. tritici (Bgt) or the hemi-biotrophic pathogen Fusarium graminearum (Fg). Levels of Hv-SGT1 showed substantial increase following treatment with H2O2 and methyl jasmonate (MeJA), only slightly induced following exposure to ethephon or abscisic acid, but not changed following exposure to salicylic acid. The demonstration that silencing of Hv-SGT1 substantially reduced resistance to Bgt indicated that Hv-SGT1 was an essential component of disease resistance in H. villosa. The over-expression of Hv-SGT1 in Yangmai 158 enhanced resistance to powdery mildew, and this correlated with increased levels of whole-cell reactive oxygen intermediates at the sites of penetration by the pathogens. Compared with wild-type plants, the expression levels of genes related to the H2O2 and JA signaling pathways were lower in the Hv-SGT1 silenced plants and higher in the Hv-SGT1 over-expressing plants. Therefore, the involvement of Hv-SGT1 in H2O2 production correlates with the hypersensitive response and jasmonic acid signaling. Our novel demonstration that wheat with over-expressed Hv-SGT1 showed enhanced resistance to both powdery mildew and FHB suggests that it could served as a transgenic genetic resource in wheat breeding for multiple disease resistance. [ABSTRACT FROM AUTHOR]

Published
2013
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