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24. Microbial synthesis of pyrroloquinoline quinone

Author

Gao, Hao, primary, Wang, Yingshan, additional, Yang, Jiahui, additional, Qiu, Min, additional, Lei, Zhixiao, additional, Zhang, Wenming, additional, Jiang, Wankui, additional, Xin, Fengxue, additional, and Jiang, Min, additional

Published
2023
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25. Metabolic Engineering of Yarrowia lipolyticafor Efficient Production of Terpenoids

Author

Wang, Jingnan, Lei, Zhixiao, Ma, Weixu, Ma, Wenqi, Jiang, Yujia, Jiang, Wankui, Wan, Yidong, Xin, Fengxue, Zhang, Wenming, and Jiang, Min

Abstract

Yarrowia lipolyticais an unconventional oleaginous yeast with special physiological characteristics. Due to its high flux of acetyl-CoA, extensive substrate spectra, clear gene sequences, and mature genetic modification tools, it has received widespread attention as a chassis strain for the production of various compounds. In this Perspective, we summarize the biosynthetic pathway of terpenoids. The advantages and application prospects of using Y. lipolyticaas chassis to produce natural products are introduced. Strategies to further improve the metabolite production efficiency are also discussed, which will provide references and guidance for further expanding application of Y. lipolytica. At present, Y. lipolyticais used to produce a variety of biochemicals. With the development of synthetic biology, the transformation tools of Y. lipolyticacan be developed in the future to improve expression capability. It can not only simplify the operation process, but also promote the production yield.

Published
2024
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26. Enhanced β-carotene production in Yarrowia lipolytica through the metabolic and fermentation engineering.

Author

Jing, Yiwen, Wang, Jingnan, Gao, Haiyan, Jiang, Yujia, Jiang, Wankui, Jiang, Min, Xin, Fengxue, and Zhang, Wenming

Subjects
CAROTENES, FERMENTATION, GENETIC overexpression, GENE expression, AGRICULTURE, MICROBIAL cells
Abstract

β-Carotene is a kind of high-value tetraterpene compound, which shows various applications in medical, agricultural, and industrial areas owing to its antioxidant, antitumor, and anti-inflammatory activities. In this study, Yarrowia lipolytica was successfully metabolically modified through the construction and optimization of β-carotene biosynthetic pathway for β-carotene production. The β-carotene titer in the engineered strain Yli-C with the introduction of the carotenogenesis genes crtI, crtE , and crtYB can reach 34.5 mg/L. With the overexpression of key gene in the mevalonate pathway and the enhanced expression of the fatty acid synthesis pathway, the β-carotene titer of the engineered strain Yli-CAH reached 87 mg/L, which was 152% higher than that of the strain Yli-C. Through the further expression of the rate-limiting enzyme tHMGR and the copy number of β-carotene synthesis related genes, the β-carotene production of Yli-C2AH2 strain reached 117.5 mg/L. The final strain Yli-C2AH2 produced 2.7 g/L β-carotene titer by fed-batch fermentation in a 5.0-L fermenter. This research will greatly speed up the process of developing microbial cell factories for the commercial production of β-carotene. One-Sentence Summary In this study, the β-carotene synthesis pathway in engineered Yarrowia lipolytica was enhanced, and the fermentation conditions were optimized for high β-carotene production. [ABSTRACT FROM AUTHOR]

Published
2023
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30. Transcriptomic Analysis Reveals the Potential Mechanisms for Improving Carotenoid Production in Rhodosporidium toruloidesZ11 under Light Stress

Author

Gao, Hao, Tang, Yunhan, Lv, Rui, Jiang, Wankui, Jiang, Yujia, Zhang, Wenming, Xin, Fengxue, and Jiang, Min

Abstract

Carotenoids, as a type of tetraterpene compound, have been widely used in food, medical, and health areas owing to their antioxidant, immune enhancement, and disease risk reduction effects. Rhodosporidium toruloidesis a promising oleaginous red yeast that can industrially synthesize carotenoids. In this study, the effects of different light exposure times and intervals on carotenoid production by R. toruloidesZ11 were first investigated. Results showed that a higher carotenoid content (1.29 mg/g) can be achieved when R. toruloidesZ11 was exposed to light for 12 h per day, which was increased by 1.98 times compared with that of dark cultivation. Transcriptome profiling revealed that light stress could effectively promote the gene expression levels of GGPS1and AL1in the carotenoid biosynthesis pathway and phrin the DNA photolysis pathway of R. toruloides. This work will provide a molecular foundation to further improve the production efficiency of carotenoids by genetic engineering.

Published
2024
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41. Biosynthesis of astaxanthin by using industrial yeast.

Author

Zhou, Dawei, Yang, Xinyi, Wang, Hanxiao, Jiang, Yujia, Jiang, Wankui, Zhang, Wenming, Jiang, Min, and Xin, Fengxue

Subjects
ASTAXANTHIN, KLUYVEROMYCES marxianus, BIOSYNTHESIS, CHEMICAL synthesis, FEED additives, YEAST, MICROBIOLOGICAL synthesis
Abstract

Astaxanthin has been widely applied in feed additives and the medical industry due to its antioxidant, coloring, and health‐promoting properties. Traditional processes for astaxanthin production mainly include natural extraction and chemical synthesis; however, they cannot meet the growing market demand and consumers' requirements for natural products. Microbial synthesis of astaxanthin offers a promising alternative. Many natural sources of astaxanthin, such as Xanthophyllomyces dendrorhous, and genetically modified yeasts, such as Saccharomyces cerevisiae, Yarrowia lipolytica and Kluyveromyces marxianus have been isolated and contructed. Various strategies such as mutagenesis, genetic modification, and fermentation regulation have been developed to increase the astaxanthin production from industrial yeast. Due to the broad substrate spectrum of industrial yeast, many low‐cost resources have also been explored for astaxanthin production. Accordingly, this review will mainly summarize the progress of research into the production of astaxanthin with yeasts, analyze factors affecting astaxanthin synthesis in metabolic pathways, and propose methods and strategies to achieve high yields of astaxanthin from yeasts. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Published
2023
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48. Strategies on improvement of pigment formation and reduction of citrinin in Monascus fermentation.

Author

Mou, Lu, Pan, Runze, Jiang, Wankui, Zhang, Wenming, Xin, Fengxue, Jiang, Yujia, and Jiang, Min

Subjects
CITRININ, MONASCUS, FERMENTATION, GENETIC engineering, GENETIC mutation, PIGMENTS, SOLID-state fermentation, COLORING matter in food
Abstract

Monasucs pigments, as natural food colorants, have worldwide popularity owing to their antibacterial ability and superior coloring characteristics, as well as various applications in food, medicine, cosmetics and several other fields. Therefore, there is a considerable demand of pigments and various approaches have been conducted to improve pigment production. However, citrinin, which causes great harm to human health, is undesirably generated during the fermentation. Thus, great efforts have been made to increase pigment production and decrease citrinin formation by culture media and condition optimization, mutation breeding or genetic engineering. For the purpose of giving insight for further research, this article makes a summary of existing study advances in Monasucs pigment production. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Published
2023
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