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Plants have evolved complex mechanisms to reprogram growth in response to drought stress. In herbaceous perennial plant species, the rhizome, which is normally an organ for propagation and food storage, can also support plant growth in stressful environments, and allows the plant to perennate and survive stress damage. However, the mechanisms that regulate rhizome growth in perennial herbs during abiotic stresses are unknown. Here, we identified a chrysanthemum (Chrysanthemum morifolium) DEAD-box RNA helicase gene, CmRH56, that is specifically expressed in the rhizome shoot apex. Knock down of CmRH56 transcript levels decreased the number of rhizomes and enhanced drought stress tolerance. We determined that CmRH56 represses the expression of a putative gibberellin (GA) catabolic gene, GA2 oxidase6 (CmGA2ox6). Exogenous GA treatment and silencing of CmGA2ox6 resulted in more rhizomes. These results demonstrate that CmRH56 suppresses rhizome outgrowth under drought stress conditions by blocking GA biosynthesis.

The paper aims to analyze the consumer joint choice behavior on fresh food purchase channels and terminal delivery services during major public health events, with the purpose of revealing the underlying influencing factors and behavioral characteristics. First, based on random utility maximization theory, the cross-nested logit model is formulated, which takes into account the influence of socioeconomic attribute factors, service attribute factors, risk perception attribute factors and trust perception attribute factors. Second, a questionnaire survey is conducted, and the obtained data are used to estimate the model parameters and perform an elasticity analysis of the utility variables. The parameter estimation results demonstrate that in the context of major public health events, consumers consider adjusting their attitudes toward e-commerce platforms first when the utility variables are altered, and fresh food purchase channels are easily replaced for consumers who choose unmanned equipment home delivery. The elasticity analysis results suggest that consumers are more willing to buy fresh food through community group-buying channels, are more sensitive to the convenience of the purchase process and are less concerned with delivery time. Although person-to-person contact increases the risk of infection, consumers still prefer attended terminal delivery services. Furthermore, consumers least agree with the effectiveness of body temperature detection methods in public places but feel that an effective way to increase consumer trust in enterprises is to strengthen personnel protection measures. [ABSTRACT FROM AUTHOR]

In this paper, a bi-population competition adaptive interior search algorithm (BCAISA) based on a reinforcement learning strategy is proposed for the classical flexible job shop scheduling problem (FJSP) to optimize the makespan. First, the scheduling solution is represented using a machine-job-based two-segment integer encoding method, and various heuristic rules are then applied to generate the initial population. Secondly, a bi-population mechanism is introduced to partition the population into two distinct sub-populations. These sub-populations are specifically tailored for machine assignment and operation permutation, employing different search strategies respectively, aiming to facilitate an efficient implementation of parallel search. A competition mechanism is introduced to facilitate the information exchange between the two sub-populations. Thirdly, the ISA is adapted for the discrete scheduling problem by discretizing a series of search operators, which include composition optimization, mirror search, and random walk. A Q-learning-based approach is proposed to dynamically adjust a key parameter, aiming to strike a balance between the capacity for global exploration and local exploitation. Finally, extensive experiments are conducted based on 10 well-known benchmark instances of the FJSP. The design of the experiment (DOE) method is employed to determine the algorithm’s parameters. Based on the computational results, the effectiveness of four improvement strategies is first validated. The BCAISA is then compared with fifteen published algorithms. The comparative data demonstrate that our algorithm outperforms other algorithms in 50% of benchmark instances. Additionally, according to the relative percentage deviation (RPD) from the state-of-the-art results, the BCAISA also exhibits superior performance. This highlights the effectiveness of our algorithm for solving the classical FJSP. To enhance the practical application, the scope of the ISA will be broadened in future work to more complex problems in real-world scenarios. [ABSTRACT FROM AUTHOR]

Body image (BI) is a multidimensional construct that refers to one's perceptions of and attitudes toward one's own physical characteristics. Adolescence is a critical developmental stage in which concerns about BI increase. Therefore, the present cross-sectional study aimed to evaluate body image and aesthetic body shape standards in a sample of middle school students living in China. The researchers gathered demographic information, as well as height and weight data, for their study. They used a body silhouette to assess body image perception and body shape aesthetics and calculated two indexes: BIP, which measures the accuracy of self-perception and the estimation of bodily dimensions, and BIS, which indicates the difference between an individual's perceived and ideal body images. A total of 1585 students in three grades at two middle schools were included in the study (759 = female, mean age = 13.67 ± 0.90; 839 = male, mean age = 13.70 ± 0.90). The results showed that the BIP bias rate of middle school students was 55.7%, and the BI dissatisfaction rate was 81.0%. Females tended to overestimate their body shape and desire to be thinner compared to males. Students with a higher BMI grading were more prone to underestimating their body shape and aspiring to be thinner. Furthermore, 8.6% of students chose underweight as the ideal body type for boys, while 22.6% chose underweight as the ideal body type for girls. In conclusion, there are significant gender differences in the aesthetic standards of body shape, and adolescents believe that for women, a thin body shape is beautiful. [ABSTRACT FROM AUTHOR]

Chicken infectious anaemia virus (CIAV) has been identified as the causative agent of chicken infectious anaemia (CIA), causing huge economic losses to the poultry industry globally. In this study, a total of 573 clinical samples were collected from 197 broiler farms in 17 provinces of China during 2020-2021. Among them, 375 samples (375/573, 65.4%) were positive for CIAV by real-time PCR. The positive rate of CIAV detection between different regions of China ranged from 46.67% (North China) to 81.25% (Central China). The nucleotide sequences of the VP1 gene were obtained for 91 CIAV strains, whole genome sequencing was successful for 72 out of 91 strains. Phylogenetic analysis based on the VP1 gene revealed that 91 CIAV strains currently circulating in China belong to three genotypes (II, IIIa and IIIb), and most of the CIAV strains belong to genotype IIIa. Phylogenetic analysis of the whole genome showed that 71 CIAV strains belong to genotype IIIa, and one strain belongs to genotype II. Sequence analysis showed several amino acid substitutions in both the VP1, VP2 and VP3 proteins. Our results enhance the understanding of the molecular characterization of CIAV infection in China. RESEARCH HIGHLIGHTS A molecular systematic survey of CIAV in China during 2020-2021. CIAV genotype IIIa is the predominant genotype in China. [ABSTRACT FROM AUTHOR]

Plants have evolved complex mechanisms to reprogram growth in response to drought stress. In herbaceous perennial plant species, the rhizome, which is normally an organ for propagation and food storage, can also support plant growth in stressful environments, and allows the plant to perennate and survive stress damage. However, the mechanisms that regulate rhizome growth in perennial herbs during abiotic stresses are unknown. Here, we identified a chrysanthemum (Chrysanthemum morifolium) DEAD-box RNA helicase gene, CmRH56 , that is specifically expressed in the rhizome shoot apex. Knock down of CmRH56 transcript levels decreased the number of rhizomes and enhanced drought stress tolerance. We determined that CmRH56 represses the expression of a putative gibberellin (GA) catabolic gene, GA2 oxidase6 (CmGA2ox6). Exogenous GA treatment and silencing of CmGA2ox6 resulted in more rhizomes. These results demonstrate that CmRH56 suppresses rhizome outgrowth under drought stress conditions by blocking GA biosynthesis. [ABSTRACT FROM AUTHOR]

Swine acute diarrhoea syndrome coronavirus (SADS‐CoV) is a newly identified swine enteropathogenic coronavirus that causes watery diarrhoea in neonatal piglets, leading to significant economic losses to the swine industry. Currently, there are no suitable serological methods to assess the infection of SADS‐CoV and effectiveness of vaccines, making an urgent need to exploit effective enzyme‐linked immunosorbent assay (ELISA) to compensate for this deficiency. Here, a recombinant plasmid that expresses the spike (S) protein of SADS‐CoV fused to the Fc domain of human IgG was constructed to generate recombinant baculovirus and expressed in HEK 293F cells. The S‐Fc protein was purified with protein G Resin, which retained reactivity with anti‐human Fc and anti‐SADS‐CoV antibodies. The S‐Fc protein was then used to develop an indirect ELISA (S‐iELISA) and the reaction conditions of S‐iELISA were optimized. As a result, the cut‐off value was determined as 0.3711 by analyzing OD450nm values of 40 SADS‐CoV‐negative sera confirmed by immunofluorescence assay (IFA) and western blot. The coefficient of variation (CV) of 6 SADS‐CoV‐positive sera within and between runs of S‐iELISA were both less than 10%. The cross‐reactivity assays demonstrated that S‐iELISA was non‐cross‐reactive with other swine viruses' sera. Furthermore, the overall coincidence rate between IFA and S‐iELISA was 97.3% based on testing 111 clinical serum samples. Virus neutralization test with seven different OD450nm values of the sera showed that the OD450nm values tested by S‐iELISA are positively correlated with the virus neutralization assay. Finally, a total of 300 pig field serum samples were tested by S‐iELISA and commercial kits of other swine enteroviruses showed that the IgG‐positive for SADS‐CoV, TGEV, PDCoV and PEDV was 81.7, 54, 65.3 and 6%, respectively. The results suggest that this S‐iELISA is specific, sensitive, repeatable and can be applied for the detection of the SADS‐CoV infection in the swine industry. [ABSTRACT FROM AUTHOR]