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32 results on '"Jiang, Chunhao"'

7. miRNA863-3p sequentially targets negative immune regulator ARLPKs and positive regulator SERRATE upon bacterial infection

Author

Niu, Dongdong, Lii, Yifan E, Chellappan, Padmanabhan, Lei, Lei, Peralta, Karl, Jiang, Chunhao, Guo, Jianhua, Coaker, Gitta, and Jin, Hailing

Subjects
Biochemistry and Cell Biology, Biological Sciences, Biotechnology, Genetics, Infectious Diseases, Infection, Arabidopsis, Arabidopsis Proteins, Base Sequence, Binding Sites, Feedback, Physiological, Gene Expression Regulation, Plant, MicroRNAs, Plant Diseases, Plant Immunity, Protein Biosynthesis, Pseudomonas syringae, RNA-Binding Proteins, Signal Transduction
Abstract

Plant small RNAs play important roles in gene regulation during pathogen infection. Here we show that miR863-3p is induced by the bacterial pathogen Pseudomonas syringae carrying various effectors. Early during infection, miR863-3p silences two negative regulators of plant defence, atypical receptor-like pseudokinase1 (ARLPK1) and ARLPK2, both lacking extracellular domains and kinase activity, through mRNA degradation to promote immunity. ARLPK1 associates with, and may function through another negative immune regulator ARLPK1-interacting receptor-like kinase 1 (AKIK1), an active kinase with an extracellular domain. Later during infection, miR863-3p silences SERRATE, which is essential for miRNA accumulation and positively regulates defence, through translational inhibition. This results in decreased miR863-3p levels, thus forming a negative feedback loop to attenuate immune responses after successful defence. This is an example of a miRNA that sequentially targets both negative and positive regulators of immunity through two modes of action to fine-tune the timing and amplitude of defence responses.

Published
2016

14. Plant growth-promoting rhizobacterium Bacillus cereus AR156 - induced systemic resistance against multiple pathogens by priming of phytoalexin synthesis and secretion

Author

Jiang, Chunhao, primary, Li, Zi-Jie, additional, Tang, Shu-Ya, additional, Ren, Jin-Yao, additional, Dong, Wen-Pan, additional, Xu, Pei-Ling, additional, Yang, Wei, additional, Zheng, Ying, additional, Yu, Yi-Yang, additional, Guo, Jianhua, additional, Luo, Yu-Ming, additional, and Niu, Dongdong, additional

Published
2022
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21. Bacillus velezensis, a potential and efficient biocontrol agent in control of pepper gray mold caused by Botrytis cinerea

Author

Hong-Kai Wang, Ming-Zi Zheng, Jiang Chunhao, Jian-Jun Xu, Liao Mengjie, and Jian-Hua Guo

Subjects
0106 biological sciences, 0301 basic medicine, biology, fungi, Hydrolase activity, Biological pest control, food and beverages, biology.organism_classification, medicine.disease_cause, 01 natural sciences, 03 medical and health sciences, Horticulture, 030104 developmental biology, Insect Science, Mold, Pepper, Plant defense against herbivory, medicine, Agronomy and Crop Science, Pathogen, Bacillus velezensis, 010606 plant biology & botany, Botrytis cinerea
Abstract

Plant gray mold disease caused by necrotrophic pathogen Botrytis cinerea, has been a serious threat to many important crops. It is urgent and necessary to seek an effective and environmentally friendly control strategy to control grey mold disease during the crop production. Moreover, it is also urgently needed to clear understand how the strategy works. To screening an efficient biocontrol agent in control of gray mold disease caused by B. cinerea, one hundred strains, which have the potential in controlling plant diseases, were selected from our laboratory’s strain library, and employed to our research. The results in the study indicated that Bacillus velezensis could act as a potential and efficient biocontrol agent (BCA), and two B. velezensis strains 5YN8 and DSN012 were screened out with high antagonistic and hydrolase activity, which could significantly control pepper gray mold disease and promote the pepper growth. Further deeply study on biocontrol mechanism indicated that B. velezensis could suppress the growth and spore formation of B. cinerea by secreting some secondary metabolites or releasing numbers of volatile organic compounds (VOCs). In the other hand, we also found that B. velezensis could trigger plant basal immunity. The BCA could increase the expression of SA signal markers and plant defense related genes. It was able to induce hydrogen peroxide accumulation and plant related enzyme activities in leaves of pepper as well. This study provides another high-efficiency, and potential strategies for the biological control of gray mold disease. It is the first report of B. velezensis strain which can control gray mold disease, In addition, it is also the first to comprehensively illustrate how the strains can efficiently protect pepper from B. cinerea attacking.

Published
2018
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28. Biofilms Positively Contribute to Bacillus amyloliquefaciens 54-induced Drought Tolerance in Tomato Plants

Author

Li-Na Zhang, Da-Cheng Wang, Jiang Chunhao, Jian-Hua Guo, Lin Chen, and Xiao-Yun Zhang

Subjects
0106 biological sciences, 0301 basic medicine, Bacillus amyloliquefaciens, Drought tolerance, Mutant, drought tolerance, Rhizobacteria, 01 natural sciences, Catalysis, abscisic acid, Inorganic Chemistry, 03 medical and health sciences, chemistry.chemical_compound, Colonization, Physical and Theoretical Chemistry, Molecular Biology, Abscisic acid, Spectroscopy, biology, Inoculation, fungi, Organic Chemistry, Biofilm, food and beverages, stomatal aperture, General Medicine, biology.organism_classification, Computer Science Applications, Horticulture, 030104 developmental biology, chemistry, biofilm formation, 010606 plant biology & botany
Abstract

Drought stress is a major obstacle to agriculture. Although many studies have reported on plant drought tolerance achieved via genetic modification, application of plant growth-promoting rhizobacteria (PGPR) to achieve tolerance has rarely been studied. In this study, the ability of three isolates, including Bacillus amyloliquefaciens 54, from 30 potential PGPR to induce drought tolerance in tomato plants was examined via greenhouse screening. The results indicated that B. amyloliquefaciens 54 significantly enhanced drought tolerance by increasing survival rate, relative water content and root vigor. Coordinated changes were also observed in cellular defense responses, including decreased concentration of malondialdehyde and elevated concentration of antioxidant enzyme activities. Moreover, expression levels of stress-responsive genes, such as lea, tdi65, and ltpg2, increased in B. amyloliquefaciens 54-treated plants. In addition, B. amyloliquefaciens 54 induced stomatal closure through an abscisic acid-regulated pathway. Furthermore, we constructed biofilm formation mutants and determined the role of biofilm formation in B. amyloliquefaciens 54-induced drought tolerance. The results showed that biofilm-forming ability was positively correlated with plant root colonization. Moreover, plants inoculated with hyper-robust biofilm (&Delta, abrB and &Delta, ywcC) mutants were better able to resist drought stress, while defective biofilm (&Delta, epsA-O and &Delta, tasA) mutants were more vulnerable to drought stress. Taken altogether, these results suggest that biofilm formation is crucial to B. amyloliquefaciens 54 root colonization and drought tolerance in tomato plants.

Published
2019
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29. Bacillus cereusNJ01 induces SA- and ABA-mediated immunity against bacterial pathogens through the EDS1-WRKY18 module

Author

Wang, Dacheng, Wei, Lirong, Ma, Jinbiao, Wan, Yingqiao, Huang, Keyi, Sun, Yiqiong, Wen, Huili, Chen, Zhipeng, Li, Zijie, Yu, Dongli, Cui, Haitao, Wu, Jingni, Wu, Yufeng, Kim, Sun Tae, Zhao, Jing, Parker, Jane E., Tsuda, Kenichi, Jiang, Chunhao, and Wang, Yiming

Abstract

Emerging evidence suggests a beneficial role of rhizobacteria in ameliorating plant disease resistance in an environment-friendly way. In this study, we characterize a rhizobacterium, Bacillus cereusNJ01, that enhances bacterial pathogen resistance in rice and Arabidopsis. Transcriptome analyses show that root inoculation of NJ01 induces the expression of salicylic acid (SA)- and abscisic acid (ABA)-related genes in Arabidopsisleaves. Genetic evidence showed that EDS1, PAD4, and WRKY18are required for B. cereusNJ01-induced bacterial resistance. An EDS1-PAD4 complex interacts with WRKY18 and enhances its DNA binding activity. WRKY18 directly binds to the W box in the promoter region of the SA biosynthesis gene ICS1and ABA biosynthesis genes NCED3and NCED5and contributes to the NJ01-induced bacterial resistance. Taken together, our findings indicate a role of the EDS1/PAD4-WRKY18 complex in rhizobacteria-induced disease resistance.

Published
2024
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32. Comparative transcriptomics reveal different genetic adaptations of biofilm formation in Bacillus subtilis isolate 1JN2 in response to Cd 2+ treatment.

Author

Yang W, Yan H, Dong G, Li Z, Jiang C, Gu D, Niu D, Zhou D, and Luo Y

Abstract

Biofilm plays important roles in the life cycle of Bacillus species, such as promoting host and object surface colonization and resisting heavy metal stress. This study utilized transcriptomics to evaluate the impacts of cadmium on the components, morphology, and function of biofilms of Bacillus subtilis strain 1JN2. Under cadmium ion stress, the morphology of the B. subtilis 1JN2 biofilm was flattened, and its mobility increased. Moreover, differential gene expression analysis showed that the main regulator of biofilm formation, Spo0A, decreased in expression under cadmium ion stress, thereby inhibiting extracellular polysaccharide synthesis through the SinI/SinR two-component regulatory system and the AbrB pathway. Cadmium ion treatment also increased the SigD content significantly, thereby increasing the expression of the flagella encoding and assembly genes in the strain. This promoted poly-γ-glutamic acid production via the DegS/DegU two-component regulatory system and the conversion of biofilm extracellular polysaccharide to poly-γ-glutamic acid. This conferred cadmium stress tolerance in the strain. Additionally, the cadmium ion-mediated changes in the biofilm composition affected the colonization of the strain on the host plant root surface. Cadmium ions also induced surfactin synthesis. These findings illustrate the potential of Bacillus species as biocontrol strains that can mitigate plant pathogenic infections and heavy metal stress. The results also provide a basis for the screening of multifunctional biocontrol strains., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Yang, Yan, Dong, Li, Jiang, Gu, Niu, Zhou and Luo.)

Published
2022
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