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Heat stress (HS) has serious negative effects on plant development and has become a major threat to agriculture. A rapid transcriptional regulatory cascade has evolved in plants in response to HS. Nuclear Factor-Y (NF-Y) complexes are critical for this mechanism, but how NF-Y complexes are regulated remains unclear. In this study, we identified NF-YC10 (NF-Y subunit C10), a central regulator of the HS response in Arabidopsis thaliana, as a substrate of SUMOylation, an important post-translational modification. Biochemical analysis showed that the SUMO ligase SIZ1 (SAP AND MIZ1 DOMAIN-CONTAINING LIGASE1) interacts with NF-YC10 and enhances its SUMOylation during HS. The SUMOylation of NF-YC10 facilitates its interaction with and the nuclear translocation of NF-YB3, in which the SUMO interaction motif (SIM) is essential for its efficient association with NF-YC10. Further functional analysis indicated that the SUMOylation of NF-YC10 and the SIM of NF-YB3 are critical for HS-responsive gene expression and plant thermotolerance. These findings uncover a role for the SIZ1-mediated SUMOylation of NF-YC10 in NF-Y complex assembly under HS, providing new insights into the role of a post-translational modification in regulating transcription during abiotic stress responses in plants.

Changes in plant auxin levels can be perceived and converted into cellular responses by auxin signal transduction. AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) proteins are auxin transcriptional inhibitors that play important roles in regulating auxin signal transduction. The stability of Aux/IAA proteins is important for transcription initiation and downstream auxin-related gene expression. Here, we report that the Aux/IAA protein IAA17 interacts with the small ubiquitin-related modifier (SUMO) E3 ligase METHYL METHANESULFONATE-SENSITIVE 21 (AtMMS21) in Arabidopsis (Arabidopsis thaliana). AtMMS21 regulated the SUMOylation of IAA17 at the K41 site. Notably, root length was suppressed in plants overexpressing IAA17, whereas the roots of K41-mutated IAA17 transgenic plants were not significantly different from wild-type roots. Biochemical data indicated that K41-mutated IAA17 or IAA17 in the AtMMS21 knockout mutant was more likely to be degraded compared with nonmutated IAA17 in wild-type plants. In conclusion, our data revealed a role for SUMOylation in the maintenance of IAA17 protein stability, which contributes to improving our understanding of the mechanisms of auxin signaling.

Salvia rosmarinus is considered a sacred plant because of its special fragrance and is commonly used in cooking and traditional medicine. Here, we report a high-quality chromosome-level assembly of the S. rosmarinus genome of 1.11 G in size; the genome has a scaffold N50 value of 95.5 Mb and contains 40,701 protein-coding genes. In contrast to other diploid Labiatae, an independent whole genome duplication (WGD) event was shown by S. rosmarinus at approximately 15 MYA. Transcriptomic comparison of two S. rosmarinus cultivars with contrasting carnosic acid (CA) content identified 842 genes significantly positively associated with carnosic acid biosynthesis in S. rosmarinus. Many of these genes have been reported to be involved in CA-biosynthesis previously, such as several MVA/MEP pathways and CYP71-coding genes. Based on the genomes and these genes, we propose a model of CA biosynthesis in S. rosmarinus. Further, comparative genome analysis of the congeneric species revealed the species-specific evolution of CA biosynthetic genes. The genes encoding diterpene synthase and the cytochromes P450 (CYPs) family of CA synthesis-associated genes form a biosynthetic gene cluster (CPSs-KSLs-CYP76AHs) responsible for the synthesis of leaf and root diterpenoids, which are located on S. rosmarinus chromosome 1 and 2, respectively. Such clustering is also observed in other sage plants, thus suggesting that genes involved in diterpenoid synthesis are conserved in the Labiataceae family. These findings provide new insights into the synthesis of aromatic terpenoids and their regulation.

Protein S-acylation is an important post-translational modification in eukaryotes, regulating the subcellular localization, trafficking, stability, and activity of substrate proteins. The dynamic regulation of this reversible modification is mediated inversely by protein S-acyltransferases and de-S-acylation enzymes, but the de-S-acylation mechanism remains unclear in plant cells. Here, we characterized a group of putative protein de-S-acylation enzymes in Arabidopsis thaliana, including 11 members of Alpha/Beta Hydrolase Domain-containing Protein 17-like acyl protein thioesterases (ABAPTs). A robust system was then established for the screening of de-S-acylation enzymes of protein substrates in plant cells, based on the effects of substrate localization and confirmed via the protein S-acylation levels. Using this system, the ABAPTs, which specifically reduced the S-acylation levels and disrupted the plasma membrane localization of five immunity-related proteins, were identified respectively in Arabidopsis. Further results indicated that the de-S-acylation of RPM1-Interacting Protein 4, which was mediated by ABAPT8, resulted in an increase of cell death in Arabidopsis and Nicotiana benthamiana, supporting the physiological role of the ABAPTs in plants. Collectively, our current work provides a powerful and reliable system to identify the pairs of plant protein substrates and de-S-acylation enzymes for further studies on the dynamic regulation of plant protein S-acylation.

In eukaryotes, the STRUCTURAL MAINTENANCE OF CHROMOSOME 5/6 (SMC5/6) complex is critical to maintaining chromosomal structures around double-strand breaks (DSBs) in DNA damage repair. However, the recruitment mechanism of this conserved complex at DSBs remains unclear. In this study, using Arabidopsis thaliana as a model, we found that SMC5/6 localization at DSBs is dependent on the protein scaffold containing INVOLVED IN DE NOVO 2 (IDN2), CELL DIVISION CYCLE 5 (CDC5), and ALTERATION/DEFICIENCY IN ACTIVATION 2B (ADA2b), whose recruitment is further mediated by DNA-damage-induced RNAs (diRNAs) generated from DNA regions around DSBs. The physical interactions of protein components including SMC5–ADA2b, ADA2b–CDC5, and CDC5–IDN2 result in formation of the protein scaffold. Further analysis indicated that the DSB localization of IDN2 requires its RNA-binding activity and ARGONAUTE 2 (AGO2), indicating a role for the AGO2–diRNA complex in this process. Given that most of the components in the scaffold are conserved, the mechanism presented here, which connects SMC5/6 recruitment and small RNAs, will improve our understanding of DNA repair mechanisms in eukaryotes.

The beerkan infiltration experiment is carried out by inserting the ring a short depth into the soil and establishing a positive head of water on the infiltration surface for at least a part of the run. Nevertheless, the data are analyzed by assuming a fully unconfined infiltration process (ring insertion depth, d = 0) and a null ponded depth of water (H = 0). The influence of ring insertion and ponded water on an infiltration process of 2 hours sampled every minute was tested in this numerical investigation. Five soils varying from sand to silt loam, three ring radii (5 - 15 cm) and the beerkan specific range of values for both d and H, that is between 0 and 1 cm were considered. The differences between the theoretical (d = H = 0) and the practical (d = H = 1 cm) setups varied from -10.4% and +8.6% for the mean infiltration rate and from -10.2% to +8.3% for the final cumulative infiltration. These differences were small and they decreased by considering a soil dependent ring radius. In particular, nearly negligible differences were detected using a small ring in coarse-textured soils and a large ring in fine-textured soils. In the coarser soils, inserting the ring and establishing a ponded depth of water did not alter appreciably the estimated coefficients of the two-parameter infiltration model with the Cumulative Linearization method since these coefficients differed between the theoretical and practical setups by no more than 9.2%; while in fine soils, linearization could not be possible regardless of the considered setup or it was the use of d = H = 1 cm instead of d = H = 0 that impeded a convincing linearization of the data. In conclusion, the satisfactory correspondence, in many circumstances, between the theoretical and the practical beerkan infiltration experiment reinforced the interest for this simple experiment as a practical means to collect infiltration data in the field. Other numerical tests should be carried out to reach more general conclusions, also considering heterogeneous soil conditions. The numerical results should represent the first step of a wider investigation that also includes laboratory and field experiments.

Stomata is an important channel for the exchange of water and gas between crops and the external environment. Stomatal behavior can shed light on how environmental stressors affect crop growth. Traditional stomata investigation methods involve experimental laboratory measurements of specific plants using stoma-related instruments or a field plot. However, developing a new remote sensing canopy resistance model for analyzing the stomatal behavior of crops under typical environmental stress is essential for regional agricultural management. Canopy resistance is a vital microscale–macroscale (from kilometers to micrometers) bridge that represents the stomatal behavior of the entire crop. In this study, a remote sensing canopy resistance model was proposed based on the Penman-Monteith model and a remote sensing evapotranspiration model. The model was verified by field-observed winter wheat data at the Yucheng Comprehensive Experiment Station (YCES) with dry-hot wind (DHW) and brackish water irrigation treatments from 2017 to 2019. The model measured canopy resistance (regression coefficient = 1.25; R2 = 0.98) well. Finally, remote sensing-based canopy resistance measurements were used to investigate the effects of soil salinity and dry-hot wind on the winter wheat. The results showed excellent model performance for retrieving crop stomatal behavior under two environmental stresses and proved that the proposed remote sensing canopy resistance model is a promising tool for the investigation of stomatal behavior under environmental stress; this is particularly relevant for regional precision farming.

The annual Zea mays ssp. mexicana L. is a member of the teosinte group and a close wild relative of maize. Thus, Zea mays ssp. mexicana L. can be effectively used in maize breeding. AtCCHA1 is a Mn2+ and/or Ca2+/H+ antiporter localized in chloroplasts in Arabidopsis. In this study, its homolog from Zea mays ssp. mexicana L., ZmmCCHA1, was isolated and characterized. The transcriptional level of ZmmCCHA1 in Zea mays ssp. mexicana L. was repressed in response to excessive Ca2+ or Mn2+. Heterologous functional complementation assays using yeast mutants showed that ZmmCCHA1 mediates Ca2+ and Mn2+ transport. The ZmmCCHA1 protein was localized in the chloroplasts when expressed in tobacco leaves. Furthermore, ectopic overexpression of ZmmCCHA1 in the Arabidopsis ccha1 mutant rescued the mutant defects on growth and the photosynthetic proteins in the thylakoid membranes. Moreover, the photosynthetic phenotypes of Arabidopsis ccha1 mutant at steady-state were greatly but not completely complemented by the overexpression of ZmmCCHA1. In addition, overexpressing the ZmmCCHA1 rescued the sensitives of PSII in the Arabidopsis ccha1 mutant to Mn2+ deficiency or high Ca2+ condition. These results indicate that the isolated ZmmCCHA1 is the homolog of AtCCHA1 and plays a conserved role in maintaining the Mn2+ and/or Ca2+ homeostasis in chloroplasts which is critical for the function of PSII in photosynthesis.

Heat stress (HS) has serious effects on plant development, resulting in heavy agricultural losses. A critical transcription factor network is involved in plant adaptation to high temperature. DEHYDRATION RESPONSIVE ELEMENT-BINDING PROTEIN2A (DREB2A) is a key transcription factor that functions in plant thermotolerance. The DREB2A protein is unstable under normal temperature and is degraded by the 26S proteasome; however, the mechanism by which DREB2A protein stability dramatically increases in response to HS remains poorly understood. In this study, we found that the DREB2A protein of Arabidopsis (Arabidopsis thaliana) is stabilized under high temperature by the posttranslational modification SUMOylation. Biochemical data indicated that DREB2A is SUMOylated at K163, a conserved residue adjacent to the negative regulatory domain during HS. SUMOylation of DREB2A suppresses its interaction with BPM2, a ubiquitin ligase component, consequently increasing DREB2A protein stability under high temperature. In addition, analysis of plant heat tolerance and marker gene expression indicated that DREB2A SUMOylation is essential for its function in the HS response. Collectively, our data reveal a role for SUMOylation in the maintenance of DREB2A stability under high temperature, thus improving our understanding of the regulatory mechanisms underlying HS response in plant cells.

With the increasing global warming, high-temperature stress is affecting plant growth and development with greater frequency. Therefore, an increasing number of studies examining the mechanism of temperature response contribute to a more optimal understanding of plant growth under environmental pressure. Post-translational modification (PTM) provides the rapid reconnection of transcriptional programs including transcription factors and signaling proteins. It is vital that plants quickly respond to changes in the environment in order to survive under stressful situations. Herein, we discuss several types of PTMs that occur in response to warm-temperature and high-temperature stress, including ubiquitination, SUMOylation, phosphorylation, histone methylation, and acetylation. This review provides a valuable resolution to this issue to enable increased crop productivity at high temperatures.

Geminiviruses are a large group of plant viruses that have been a serious threat to worldwide agriculture. Transcription of the virus-encoded genes is necessary for geminiviruses to complete their life cycle, but the host proteins which directly target geminivirus promoters for suppression of viral gene transcription remain to be identified. Using Beet severe curly top virus (BSCTV) which causes severe plant symptoms as a system, we performed a yeast one-hybrid screening and identified ABA INSENSITIVE 5 (ABI5), a critical transcription factor in Abscisic acid (ABA) signaling transduction, as an interactor with the viral promoter. Further data showed that an ABA-responsive element in the viral promoter is necessary for its interaction with ABI5 and symptom development. Overexpression of ABI5 suppresses the transcription activity of the viral promoter and BSCTV infection in Nicotiana benthamiana and Arabidopsis; whilst depletion of ABI5 enhances the infection of BSCTV in Arabidopsis. Taken together, our study uncovered the function of ABI5 in the plant-virus interaction and will provide us with a new strategy to protect crops from geminivirus infection.

Geminiviruses are a large group of single-stranded DNA viruses that infect plants and cause severe agricultural losses worldwide. Given geminiviruses only have small genomes that encode a few proteins, viral factors have to interact with host components to establish an environment suitable for virus infection, whilst the host immunity system recognizes and targets these viral components during infection. Post-translational protein modifications, such as phosphorylation, lipidation, ubiquitination, SUMOylation, acetylation, and methylation, have been reported to be critical during the interplay between host plants and geminiviruses. Here we summarize the research progress, including phosphorylation and lipidation which usually control the activity and localization of viral factors; as well as ubiquitination and histone modification which are predominantly interfered with by viral components. We also discuss the dynamic competition on protein modifications between host defense and geminivirus efficient infection, as well as potential applications of protein modifications in geminivirus resistance. The summary and perspective of this topic will improve our understanding on the mechanism of geminivirus-plant interaction and contribute to further protection of plants from virus infection. This article is protected by copyright. All rights reserved.

SUMOylation is a critical post-translational modification that regulates the nature and activity of protein substrates. The reaction is usually enhanced by a SIZ/PIAS-type of SUMO E3 ligase, but the functions of its homologs in maize have not yet been reported. In this study, we functionally characterized three members of this family of SUMO ligases, ZmSIZ1a, ZmSIZ1b, and ZmSIZ1c, from Zea mays. These maize SIZ1 homologs harbor conserved domains and structures with AtSIZ1, suggesting that they are potential functional SUMO ligases, which is supported by further biochemical data. The expression of these maize SIZ1 genes was detectable ubiquitously in different maize tissues and was usually induced by abiotic stresses. Expression of ZmSIZ1 members complements the leaf developmental defects of the AtSIZ1 mutant, suggesting their conserved function in development regulation. Interestingly, overexpression of ZmSIZ1c, but not ZmSIZ1a or ZmSIZ1b, in the wild-type Arabidopsis resulted in early flowering, implying that these members differ in terms of flowering control. Besides, overexpression of these ZmSIZ1 genes also improved salt tolerance in Arabidopsis. Collectively, our functional characterization of the ZmSIZ1 members provides hints for further investigation on the functions of SUMOylation in the development and stress responses in maize.

The SWI/SNF complex is crucial to chromatin remodeling in various biological processes in different species, but the distinct functions of its components in plant development remain unclear. Here we uncovered the role of SWI3B, a subunit of the Arabidopsis thaliana SWI/SNF complex, via RNA interference. Knockdown of SWI3 B resulted in an upward-curling leaf phenotype. Further investigation showed that the RNA level of IAA carboxyl methyltransferase 1 (IAMT1), encoding an enzyme involved in auxin metabolism, was dramatically elevated in the knockdown (SWI3B-RNAi) plants. In addition, activation of IAMT1 produced a leaf-curling phenotype similar to that of the SWI3B-RNAi lines. Database analysis suggested that the last intron of IAMT contains a site of polymerase V (Pol V) stabilized nucleosome, which may be associated with SWI3B. Data from a micrococcal nuclease (MNase) digestion assay showed that nucleosome occupancy around this site was downregulated in the leaves of SWI3B-RNAi plants. In addition, knockdown of IAMT1 in the SWI3B-RNAi background repressed the abnormal leaf development. Thus, SWI3B-mediated chromatin remodeling is critical in regulating the expression of IAMT1 in leaf development.

Geminivirus infection results in systemic symptoms in plants, but the mechanism by which the virus affects cell-to-cell signalling involved in development and resistance remains unclear. Based on recent evidence, we suggest a potential mechanism that geminivirus C4 proteins interfere with intercellular signals by interacting with receptor-like kinases on the plasma membrane.

SUMOylation, which transfers the Small Ubiquitin-related Modifier (SUMO) polypeptides to target proteins, regulates diverse cellular processes in eukaryotes. The SUMO conjugation reaction is usually promoted by SUMO E3 ligases, but the molecular functions of this type of enzymes remain unclear in cereal crops. Here, OsMMS21, a SUMO E3 ligase, was functionally characterized in rice (Oryza sativa). Bioinformatics analysis showed that OsMMS21 harbors a conserved SP-RING domain that is essential for the activity of SUMO ligases. Biochemical data indicated that this protein is auto-SUMOylated. Besides, overexpression of OsMMS21 rescued the developmental defects of the AtMMS21 mutant, supporting that OsMMS21 is a functional homolog of the Arabidopsis SUMO ligase AtMMS21. The OsMMS21 rice T-DNA mutant displays a short-root and dwarfism phenotype. RNA-seq data revealed that the expression levels of many genes involved in signaling transduction of hormones, including auxin, are altered in the OsMMS21 mutant. Further results under the auxin treatment showed that the OsMMS21 mutant is insensitive to auxin. Collectively, our results demonstrated the molecular features of OsMMS21 and uncovered the roles of this SUMO ligase in development and auxin response, providing hints for further studies on protein SUMOylation in rice.

The novel columnar liquid crystals based on symmetric hairpin-shaped cholesterol tetramers bridged by rigid or hydrogen-bonding Schiff-base spacers were prepared by reacting cholesterol dimer with phenylenediamines or dihydrazides in yields of 70–85%. Structural characterisation of these new compounds had been achieved by infrared, nuclear magnetic resonance and mass spectrometry. Their mesomorphic properties were investigated by polarising optical microscopy, differential scanning calorimetry and X-ray diffraction. They exhibited good hexagonal columnar mesophase with three molecules in a disc of the columnar hexagonal state. This study implied that the symmetric cholesterol tetramers with rigid cores or hydrogen-bonding cores prefer to columnar mesophase.

The annual Zea mays ssp. mexicana L., a member of the teosinte group, is a close wild relative of maize and thus can be effectively used in maize improvement. In this study, an ICE-like gene, ZmmICE1, was isolated from a cDNA library of RNA-Seq from cold-treated seedling tissues of Zea mays ssp. mexicana L. The deduced protein of ZmmICE1 contains a highly conserved basic helix-loop-helix (bHLH) domain and C-terminal region of ICE-like proteins. The ZmmICE1 protein localizes to the nucleus and shows sumoylation when expressed in an Escherichia coli reconstitution system. In addition, yeast one hybrid assays indicated that ZmmICE1 has transactivation activities. Moreover, ectopic expression of ZmmICE1 in the Arabidopsis ice1-2 mutant increased freezing tolerance. The ZmmICE1 overexpressed plants showed lower electrolyte leakage (EL), reduced contents of malondialdehyde (MDA). The expression of downstream cold related genes of Arabidopsis C-repeat-binding factors (AtCBF1, AtCBF2 and AtCBF3), cold-responsive genes (AtCOR15A and AtCOR47), kinesin-1 member gene (AtKIN1) and responsive to desiccation gene (AtRD29A) was significantly induced when compared with wild type under low temperature treatment. Taken together, these results indicated that ZmmICE1 is the homolog of Arabidopsis inducer of CBF expression genes (AtICE1/2) and plays an important role in the regulation of freezing stress response.

Novel calix[4]resorcinarene derivatives with four cholesterol units bridging by soft spacers or rigid Schiff-base spacers were prepared in yields of 63–76%. The hexagonal columnar liquid crystalline behaviors were firstly observed for calix[4]resorcinarene derivatives. The mesomorphic properties were influenced greatly by the structures of spacers. The rigid spacers were favorable for enhancing the phase transition temperatures and enlarging the temperature scopes of mesophase.

Leaf area index (LAI) is a key biophysical parameter for monitoring crop growth status, predicting crop yield, and quantifying crop variability in agronomic applications. Mapping the LAI at the field scale using multispectral cameras onboard unmanned aerial vehicles (UAVs) is a promising precision-agriculture application with specific requirements: The LAI retrieval method should be (1) robust so that crop LAI can be estimated with similar accuracy and (2) easy to use so that it can be applied to the adjustment of field management practices. In this study, three UAV remote-sensing missions (UAVs with Micasense RedEdge-M and Cubert S185 cameras) were carried out over six experimental plots from 2018 to 2019 to investigate the performance of reflectance-based lookup tables (LUTs) and vegetation index (VI)-based LUTs generated from the PROSAIL model for wheat LAI retrieval. The effects of the central wavelengths and bandwidths for the VI calculations on the LAI retrieval were further examined. We found that the VI-LUT strategy was more robust and accurate than the reflectance-LUT strategy. The differences in the LAI retrieval accuracy among the four VI-LUTs were small, although the improved modified chlorophyll absorption ratio index-lookup table (MCARI2-LUT) and normalized difference vegetation index-lookup table (NDVI-LUT) performed slightly better. We also found that both of the central wavelengths and bandwidths of the VIs had effects on the LAI retrieval. The VI-LUTs with optimized central wavelengths (red = 612 nm, near-infrared (NIR) = 756 nm) and narrow bandwidths (~4 nm) improved the wheat LAI retrieval accuracy (R2 ≥ 0.75). The results of this study provide an alternative method for retrieving crop LAI, which is robust and easy use for precision-agriculture applications and may be helpful for designing UAV multispectral cameras for agricultural monitoring.

Geminiviruses are DNA viruses that cause severe diseases in diverse species of plants, resulting in considerable agricultural losses worldwide. C4 proteins are a major symptom determinant in several geminiviruses, including Beet severe curly top virus (BSCTV). Here, we uncovered a novel mechanism by which danger peptide signaling enhances the internalization of BSCTV C4 in plant cells. Previous studies showed that this signaling is important for activation of bacterium- and fungus-triggered immune responses, but its function in plant–virus interactions was previously unknown. Pep1 RECEPTOR1 (PEPR1) and PEPR2 are receptor kinases recognized by Peps (plant elicitor peptides) in the danger peptide pathway. We found that BSCTV C4 up-regulated and interacted with PEPR2 but not PEPR1. The Pep1–PEPR2 complex stimulated the internalization of C4 in both Arabidopsis and Nicotiana benthamiana cells. Furthermore, C4 induced callus formation in Arabidopsis, which was suppressed by PEPR2 overexpression but enhanced in the pepr2 mutants. In the presence of Pep1, overexpression of PEPR2 suppressed BSCTV infection in N. benthamiana. Exogenous Pep1 also reduced BSCTV infection in Arabidopsis in a PEPR2-dependent manner. Thus, PEPR2 recognizes the symptom determinant C4 and enhances its internalization mediated by danger peptides, suppressing BSCTV infection.

DNA damage decreases genome stability and alters genetic information in all organisms. Conserved protein complexes have been evolved for DNA repair in eukaryotes, such as the structural maintenance complex 5/6 (SMC5/6), a chromosomal ATPase involved in DNA double-strand break (DSB) repair. Several factors have been identified for recruitment of SMC5/6 to DSBs, but this complex is also associated with chromosomes under normal conditions; how SMC5/6 dissociates from its original location and moves to DSB sites is completely unknown. In this study, we determined that SWI3B, a subunit of the SWI/SNF complex, is an SMC5-interacting protein in Arabidopsis thialiana. Knockdown of SWI3B or SMC5 results in increased DNA damage accumulation. During DNA damage, SWI3B expression is induced, but the SWI3B protein is not localized at DSBs. Notably, either knockdown or overexpression of SWI3B disrupts the DSB recruitment of SMC5 in response to DNA damage. Overexpression of a cotranscriptional activator ADA2b rescues the DSB localization of SMC5 dramatically in the SWI3B-overexpressing cells but only weakly in the SWI3B knockdown cells. Biochemical data confirmed that ADA2b attenuates the interaction between SWI3B and SMC5 and that SWI3B promotes the dissociation of SMC5 from chromosomes. In addition, overexpression of SMC5 reduces DNA damage accumulation in the SWI3B knockdown plants. Collectively, these results indicate that the presence of an appropriate level of SWI3B enhances dissociation of SMC5 from chromosomes for its further recruitment at DSBs during DNA damage in plant cells.

SUMOylation is an important protein modification that regulates the properties of substrate proteins in a variety of cellular processes. SUMOylation is catalyzed via a cascade of enzymes and is usually stimulated by SUMO E3 ligases. However, the molecular functions and regulatory mechanisms of SUMOylation in forage crops are unknown. Here, we isolated and functionally characterized DiMMS21, a homolog of the Arabidopsis thaliana SUMO ligase AtMMS21, from the forage legume Desmodium intortum. DiMMS21 is expressed ubiquitously in various D. intortum organs and its encoded protein is found in the cytoplasm and nucleus. Bioinformatics analysis indicated that DiMMS21 contains a conserved SP-RING domain that is required for its activity. Biochemical evidence supports the notion that this protein is a functional SUMO ligase. When expressed in an Arabidopsis mms21 mutant, DiMMS21 completely rescued the defects in root, leaf, and silique development. The results from cotyledon greening and marker gene expression suggested that DiMMS21 can only partially complements the role of AtMMS21 in abscisic acid (ABA) responses. In summary, we characterized the molecular features of DiMMS21 and uncovered potential roles of this SUMO ligase in development and ABA responses, increasing our understanding on the function of SUMOylation in forage crops.

Abundant shallow underground brackish water resources could help in alleviating the shortage of fresh water resources and the crisis concerning agricultural water resources in the North China Plain. Improper brackish water irrigation will increase soil salinity and decrease the final yield due to salt stress affecting the crops. Therefore, it is urgent to develop a practical and low-cost method to monitor the soil salinity of brackish irrigation systems. Remotely sensed spectral vegetation indices (SVIs) of crops are promising proxies for indicating the salinity of the surface soil layer. However, there is still a challenge concerning quantitatively correlating SVIs with the salinity of deeper soil layers, in which crop roots are mainly distributed. In this study, a field experiment was conducted to investigate the relationship between SVIs and salinity measurements at four soil depths within six winter wheat plots irrigated using three salinity levels at the Yucheng Comprehensive Experimental Station of the Chinese Academy of Sciences during 2017–2019. The hyperspectral reflectance was measured during the grain-filling stage of winter wheat, since it is more sensitive to soil salinity during this period. The SVIs derived from the observed hyperspectral data of winter wheat were compared with the salinity at four soil depths. The results showed that the optimized SVIs, involving soil salt-sensitive blue, red-edge, and near-infrared wavebands, performed better when retrieving the soil salinity (R2 ≥ 0.58, root mean square error (RMSE) ≤ 0.62 g/L), especially at the 30-cm depth (R2 = 0.81, RMSE = 0.36 g/L). For practical applications, linear or quadratic models based on the screened SVIs in the form of normalized differential vegetation indices (NDVIs) could be used to retrieve soil salinity (R2 ≥ 0.63, RMSE ≤ 0.62 g/L) at all soil depths and then diagnose salt stress in winter wheat. This could provide a practical technique for evaluating regional brackish water irrigation systems.

The post-translational protein modification known as SUMOylation has conserved roles in the heat stress responses of various species. The functional connection between the global regulation of gene expression and chromatin-associated SUMOylation in plant cells is unknown. Here, we uncovered a genome-wide relationship between chromatin-associated SUMOylation and transcriptional switches in Arabidopsis thaliana grown at room temperature, exposed to heat stress, and exposed to heat stress followed by recovery. The small ubiquitin-like modifier (SUMO)-associated chromatin sites, characterized by whole-genome ChIP-seq, were generally associated with active chromatin markers. In response to heat stress, chromatin-associated SUMO signals increased at promoter-transcriptional start site regions and decreased in gene bodies. RNA-seq analysis supported the role of chromatin-associated SUMOylation in transcriptional activation during rapid responses to high temperature. Changes in SUMO signals on chromatin were associated with the upregulation of heat-responsive genes and the downregulation of growth-related genes. Disruption of the SUMO ligase gene SIZ1 abolished SUMO signals on chromatin and attenuated rapid transcriptional responses to heat stress. The SUMO signal peaks were enriched in DNA elements recognized by distinct groups of transcription factors under different temperature conditions. These observations provide evidence that chromatin-associated SUMOylation regulates the transcriptional switch between development and heat stress response in plant cells., Whole-genome ChIP-seq in Arabidopsis reveals that the distribution of SUMO-associated signals on chromatin changes in response to heat stress and is associated with the activation and repression of gene transcription. This type of chromatin- associated SUMOylation depends primarily on SUMO ligase SIZ1 and controls the transcriptional switch between plant development and heat stress responses.

Calcium is important for chloroplast, not only in its photosynthetic but also nonphotosynthetic functions. Multiple Ca 2+ /H + transporters and channels have been described and studied in the plasma membrane and organelle membranes of plant cells; however, the molecular identity and physiological roles of chloroplast Ca 2+ /H + antiporters have remained unknown. Here we report the identification and characterization of a member of the UPF0016 family, CCHA1 (a chloroplast-localized potential Ca 2+ /H + antiporter), in Arabidopsis thaliana . We observed that the ccha1 mutant plants developed pale green leaves and showed severely stunted growth along with impaired photosystem II (PSII) function. CCHA1 localizes to the chloroplasts, and the levels of the PSII core subunits and the oxygen-evolving complex were significantly decreased in the ccha1 mutants compared with the wild type. In high Ca 2+ concentrations, Arabidopsis CCHA1 partially rescued the growth defect of yeast gdt1Δ null mutant, which is defective in a Ca 2+ /H + antiporter. The ccha1 mutant plants also showed significant sensitivity to high concentrations of CaCl 2 and MnCl 2 , as well as variation in pH. Taken these results together, we propose that CCHA1 might encode a putative chloroplast-localized Ca 2+ /H + antiporter with critical functions in the regulation of PSII and in chloroplast Ca 2+ and pH homeostasis in Arabidopsis .

Determination of soil hydraulic parameters at field scale is of great importance for modeling soil water dynamics and for agricultural water management practice. The parameter regionalization has been a hot topic for many decades in soil science and hydropedology. Based on the statistical description and spatial structure of the soil physical and hydraulic properties measured via soil sampling in the field, three inverse modeling approaches had been proposed to obtain the effective hydraulic parameters. The validity and effectiveness of the three approaches had been discussed by designing four simulation scenarios (a 'fine scale solution' plus the three upscaling approaches). For each inverse modeling, the soil water distribution along the profiles and their dynamics during seven growing seasons (from the year of 2000 to 2006) in the study area had been simulated through the combined use of HYDRUS-1D and PEST. Results demonstrated that the effective soil hydraulic parameters derived from all the three approaches were comparable and fairly close to the 'fine scale solution'. Although, statistics of the hydraulic parameters indicated that the median of K-s of first soil layer, as compared to that of other layers, was most closest to the effective K-s values that were obtained through the three upscaling approaches; while the median of theta(s) values for the top three layers was close to the effective theta(s) values in scenario 2 and scenario 3, but fairly smaller than that in scenario 4. The soil water dynamics were not sensitive to the residual soil water content (theta(r)), even though the theta(r) showed quite different distribution pattern from that of K-s and theta(s). In conclusion, the practice of combining the PEST with the HYDRUS-1D provided an effective and reasonable method to inversely determine the effective hydraulic parameters of the equivalent soil profile in the field. (C) 2015 Elsevier B.V. All rights reserved.

Low-cost, simple, rapid and accurate approaches for measuring soil hydraulic properties are of great importance in the application of hydrologic models of the vadose zone. A recently proposed method of constant-saturation absorption (MCSA) for determining Brooks-Corey (BC) model parameters is promising. However, this method has not been tested using experimental data. In this paper, measured soil-water diffusivity or soil-water retention curves (SWRCs) of 20 soil samples were used to test the reliability of MCSA. The results indicate that MCSA consistently estimated soil-water diffusivities by the Bruce and Klute method, but it substantially overestimated air-entry suctions (h(d)) and thereby soil suction. A new method (MCPA) is proposed herein to overcome the problems associated with MCSA. Using the improved method, estimated SWRCs are consistent with observation. With the estimated soil hydraulic parameters, HYDRUS-1D generated very accurate simulations of the cumulative absorption curves. Compared with direct or other indirect methods, MCPA is a more accurate, low-cost, and simple method, and it is especially useful for rapid determination of SWRC for soil-water movement simulations. However, if unsaturated soil-water diffusivity is of greater concern, our results also indicate that MCSA more accurately predicts this quantity and thus should be preferred. (C) 2015 Elsevier B.V. All rights reserved

The 26S proteasome is a multi-subunit protease controlling most of the cytosolic and nuclear protein turnover, regulating many cellular events in eukaryotes. However, functional modification on this complex remains unclear. Here, we showed a novel mechanism that a SUMO ligase AtMMS21 regulates activity of the 26S proteasome in root development of Arabidopsis. Our in vitro and in vivo data supported that AtMMS21 interacts with RPT2a, a subunit of the 26S proteasome. The mutants of AtMMS21 and RPT2a display similar developmental defect of roots, suggesting their association in this process. In addition, RPT2a is modified by SUMO3, potentially related to AtMMS21. During development, the activity of the 26S proteasome is lower in both mutants of AtMMS21 and RPT2a, compared with that of wild type. Furthermore, the protein level but not the RNA level of RPT2a is decreased in the absence of AtMMS21, implying stability regulation of the proteasome complex through the AtMMS21-RPT2a interaction. Taken together, the current study would improve our understanding on the regulatory mechanism of the 26S proteasome via protein modification in root development.

DNA damage occurs in all cells and can hinder chromosome stability and cell viability. Structural Maintenance of Chromosomes5/6 (SMC5/6) is a protein complex that functions as an evolutionarily conserved chromosomal ATPase critical for repairing DNA double-strand breaks (DSBs). However, the mechanisms regulating this complex in plants are poorly understood. Here, we identified the transcriptional coactivator ALTERATION/DEFICIENCY IN ACTIVATION2B (ADA2b) as an interactor of SMC5 in Arabidopsis (Arabidopsis thaliana). ADA2b is a conserved component of the Spt-Ada-Gcn5 acetyltransferase complex, which functions in transcriptional regulation. Characterization of mutant and knockdown Arabidopsis lines showed that disruption of either SMC5 or ADA2b resulted in enhanced DNA damage. Both SMC5 and ADA2b were associated with γ-H2AX, a marker of DSBs, and the recruitment of SMC5 onto DSBs was dependent on ADA2b. In addition, overexpression of SMC5 in the ada2b mutant background stimulated cell death. Collectively, our results show that the interaction between ADA2b and SMC5 mediates DNA repair in plant cells, suggesting a functional association between these conserved proteins and further elucidating mechanisms of DNA damage repair in plants.

S-acylation of the beet severe curly top virus C4 protein is essential for its membrane association, interaction with CLV1, and symptom determination during geminivirus infection., Geminiviruses, such as beet severe curly top virus (BSCTV), are a group of DNA viruses that cause severe plant diseases and agricultural losses. The C4 protein is a major symptom determinant in several geminiviruses; however, its regulatory mechanism and molecular function in plant cells remain unclear. Here, we show that BSCTV C4 is S-acylated in planta, and that this post-translational lipid modification is necessary for its membrane localization and functions, especially its regulation of shoot development of host plants. Furthermore, the S-acylated form of C4 interacts with CLAVATA 1 (CLV1), an important receptor kinase in meristem maintenance, and consequentially affects the expression of WUSCHEL, a major target of CLV1. The abnormal development of siliques in Arabidopsis thaliana infected with BSCTV is also dependent on the S-acylation of C4, implying a potential role of CLAVATA signaling in this process. Collectively, our results show that S-acylation is essential for BSCTV C4 function, including the regulation of the CLAVATA pathway, during geminivirus infection.

Reference evapotranspiration (ET0) is the key factor for hydrologic water balance, irrigation scheduling, and water resources planning. Based on Food and Agriculture Organization (FAO) Penman–Monteith method and the climate variables of 57 meteorological stations from 1960 to 2010 in southwest China, the spatial and temporal distributions of ET0 were analyzed by using Mann–Kendall test and Sen’s slope estimator. Sensitivity coefficient was used to analyze the sensitivities of ET0 to four climate variables, and the key climate variables attributed to ET0 change were determined. Result showed that there was a slight downward trend of ET0 from 1960 to 2010 and spatially increasing trend from northeast to southwest in annual time scale. Results also showed that ET0 had relatively higher sensitivity to wind speed and mean air temperature, and wind speed was the dominant variable for change of ET0 in southwest China. The inverse relationship between increasing air temperature and decreasing evaporation, “evaporation paradox,” existed in southwest China, and the negative contribution of wind speed to the changes of ET0 offset the positive contribution of air temperature.

The first calix[4]arene-cholesterol liquid crystals were designed and synthesized in yields of 50–80%. Structural and conformational characterization of these new compounds had been achieved by NMR, MS, and elemental analysis. Their liquid crystalline behaviors were studied by polarizing optical microscopy, differential scanning calorimetry, and X-ray diffraction. All calix[4]arene derivatives with two or four cholesterol units show excellent mesomorphic properties of the columnar molecular arrangement of the calixarenes bowlic columns with cholesterol units as ancillary lateral columns. The long spacer and more cholesterol units were favorable for excellent mesophase.

HYDRUS-1D was combined with lysimeter experiments to study extinction depth and steady water storage in root zone (Ws) of groundwater evaporation (ETgw) under winter wheat and silt soil. The measured soil water contents and daily ETgw with various groundwater depths were used to calibrate and validate the parameters in HYDRUS-1D. In total, 13 groundwater depths ranging from 0.5 to 5.0 m were set up for scenario simulation to determine the extinction depth and Ws. The results showed that HYDRUS-1D had an acceptable performance in simulating the soil water storage in the 0–60 cm layer and the daily ETgw. Moreover, the ETgw decreased linearly with increasing groundwater depth from 0.5 to 2.5 m and decreased as a power function with increasing groundwater depth from 2.5 to 5.0 m. Under the condition of winter wheat and silt soil, the extinction depth of ETgw was about 5.0 m. Ws decreased linearly with increasing groundwater depth from 0.7 to 2.0 m, but was not influenced further by the groundwater at depths beyond 2.0 m.

In irrigation districts with shallow water table, groundwater evaporation (ETgw) and groundwater depth are important variables for the study of water balance. These variables are influenced by natural factors and human activity. In this study, a new method of simulating ETgw was used for SWAT, and the computing and output modules of groundwater depth were established. In addition, based on simulations, we analyzed the influences of irrigation schedules and soil types on ETgw and groundwater depth in the Irrigation Districts along the Lower Reach of Yellow River Basin. The applicabilities of the three methods (Hargreaves, Priestley-Taylor and Penman-Monteith) simulating potential evapotranspiration in SWAT 2000 were evaluated by the measured groundwater evaporation, and Hargreaves was applied in the simulation for irrigation district due to its smallest simulation error. The results presented that the mean groundwater depth decreased with the increase of irrigation amount. However, the changes of soil water storage in root zone and groundwater depth might have more complex effects on the variation of the mean groundwater evaporation. Loamy sand had the smallest annual mean groundwater depth and mean annual ETgw in the study area, while silt loam corresponded to the largest groundwater depth and groundwater evaporation. This study provides a new method for making simulations and analyses of the hydrological cycle in irrigation districts which has a shallow water table.