Search

Satellite Interferometric Synthetic Aperture Radar (InSAR) is widely used for topographic, geological and natural resource investigations. However, most of the existing InSAR studies of ground deformation are based on relatively short periods and single sensors. This paper introduces a new multi-sensor InSAR time series data fusion method for time-overlapping and time-interval datasets, to address cases when partial overlaps and/or temporal gaps exist. A new Power Exponential Knothe Model (PEKM) fits and fuses overlaps in the deformation curves, while a Long Short-Term Memory (LSTM) neural network predicts and fuses any temporal gaps in the series. Taking the city of Wuhan (China) as experiment area, COSMO-SkyMed (2011–2015), TerraSAR-X (2015–2019) and Sentinel-1 (2019–2021) SAR datasets were fused to map long-term surface deformation over the last decade. An independent 2011–2020 InSAR time series analysis based on 230 COSMO-SkyMed scenes was also used as reference for comparison. The correlation coefficient between the results of the fusion algorithm and the reference data is 0.87 in the time overlapping region and 0.97 in the time-interval dataset. The correlation coefficient of the overall results is 0.78, which fully demonstrates that the algorithm proposed in our paper achieves a similar trend as the reference deformation curve. The experimental results are consistent with existing studies of surface deformation at Wuhan, demonstrating the accuracy of the proposed new fusion method to provide robust time series for the analysis of long-term land subsidence mechanisms.

Abstract Background With a series of clinical trials confirming the sensitivity of small cell lung cancer (SCLC) to immunotherapy, research on personalized treatment for SCLC has gained increasing attention. Currently, the most widely accepted subtype of SCLC is based on the expression levels of Achaete-Scute Family BHLH Transcription Factor 1 (ASCL1), neurogenic differentiation factor 1 (NEUROD1), and POU class 2 homeobox 3 (POU2F3). However, real-world studies on this classification remain limited. Methods We retrospectively collected biopsy specimens from patients who received immunotherapy at Shandong Provincial Hospital between January 2019 and July 2021. After determining the patient subtypes using immunohistochemistry, we analyzed the relationships between each subtype and survival as well as some clinical characteristics. Results In our study, we found that the subtype I achieved a significant survival advantage compared to the other groups. Additionally, the subtype A demonstrated a significant survival disadvantage. Among patients in the subtype I, there was a higher proportion of early brain metastasis and patients with a family history of tumors, while the subtype A had a lower proportion. Furthermore, the subtype A exhibited relatively poor immune infiltration. Conclusion In a diverse cohort of SCLC patients receiving immunotherapy, the subtype-I showed significant survival advantages while the subtype-A experienced a worse survival.

Abstract Aims Surveys and research on the applications of the hepatic venous pressure gradient (HVPG) are important for understanding the current status and future development of this technology in China. This article aimed to investigate the status of hepatic venous pressure gradient measurement in China in 2022. Methods We investigated the overall status of HVPG technology in China—including hospital distribution, hospital level, annual number of cases, catheters used, average cost, indications, and current challenges by using online questionnaire. By counting the number and percentages of cases of these results, we hope to clarify the current status of HVPG measurements in China. Results According to the survey, 85 hospitals in China used HVPG technology in 2022 distributed across 29 provinces. A total of 4989 HVPG measurements were performed in all of the surveyed hospitals in 2022, of which 2813 cases (56.4%) were measured alone. The average cost of HVPG measurement was 5646.8 ± 2327.9 CNY. Of the clinical teams who performed the measurements (sometimes multiple per hospital), 94.3% (82/87) used the balloon method, and the majority of the teams (72.4%, 63/87) used embolectomy catheters. Conclusions This survey clarified the clinical application status of HVPG in China and confirmed that some medical institutions in China have established a foundation for this technology. It is still necessary to continue promoting and popularizing this technology in the future.

This is an article in the field of process mineralogy. In order to solve the problem of color spot impurity sorting in high-purity mica, a sorting method of mica containing impurities based on image processing is proposed in this article. According to the state of color spot impurities of mica under different light sources, the influence of image illumination environment is studied. On this basis, the processing methods such as Gaussian filtering and Canny edge detection are studied. Finally, the image recognition and discrimination of mica ores are realized accurately, and good results are obtained in the recognition of three different types of mica ores.

Abstract Purpose This pilot study aimed to investigate the effects of REX exoskeleton rehabilitation robot training on the balance and lower limb function in patients with sub-acute stroke. Methods This was a pilot, single-blind, randomized controlled trial. Twenty-four patients with sub-acute stroke (with the course of disease ranging from 3 weeks to 3 months) were randomized into two groups, including a robot group and a control group. Patients in control group received upright bed rehabilitation (n = 12) and those in robot group received exoskeleton rehabilitation robot training (n = 12). The frequency of training in both groups was once a day (60 min each) for 5 days a week for a total of 4 weeks. Besides, the two groups were evaluated before, 2 weeks after and 4 weeks after the intervention, respectively. The primary assessment index was the Berg Balance Scale (BBS), whereas the secondary assessment indexes included the Fugl-Meyer Lower Extremity Motor Function Scale (FMA-LE), the Posture Assessment Scale for Stroke Patients (PASS), the Activities of Daily Living Scale (Modified Barthel Index, MBI), the Tecnobody Balance Tester, and lower extremity muscle surface electromyography (sEMG). Results The robot group showed significant improvements (P

Abstract Tungsten trioxide (WO3) has been recognized as the most promising photocatalyst for highly selective oxidation of methane (CH4) to formaldehyde (HCHO), but the origin of catalytic activity and the reaction manner remain controversial. Here, we take {001} and {110} facets dominated WO3 as the model photocatalysts. Distinctly, {001} facet can readily achieve 100% selectivity of HCHO via the active site mechanism whereas {110} facet hardly guarantees a high selectivity of HCHO along with many intermediate products via the radical way. In situ diffuse reflectance infrared Fourier transform spectroscopy, electron paramagnetic resonance and theoretical calculations confirm that the competitive chemical adsorption between CH4 and H2O and the different CH4 activation routes on WO3 surface are responsible for diverse CH4 oxidation pathways. The microscopic mechanism elucidation provides the guidance for designing high performance photocatalysts for selective CH4 oxidation.

Since the survival of lymphoma patients who experience disease progression or relapse remains very poor, new therapeutic approaches and effective drugs are urgently needed. Here we show that auranofin (AF), an anti-rheumatoid drug thought to inhibit thioredoxin reductases (TXNRDs) as its mechanism of action, exhibited potent activity against multiple cancer types, especially effective against B cell lymphoma. Surprisingly, a knockdown of TXNRD1 and TXNRD2 did not cause significant cytotoxicity, suggesting that abrogation of TXNRD enzyme per se was insufficient to cause cancer cell death. Further mechanistic study showed that the interaction of AF with TXNRD could convert this antioxidant enzyme to a ROS-generating molecule via disrupting its electron transport, leading to a leak of electrons that interact with molecular oxygen to form superoxide. AF also suppressed energy metabolism by inhibiting both mitochondria complex II and the glycolytic enzyme GAPDH, leading to a significant depletion of ATP and inhibition of cancer growth in vitro and in vivo. Importantly, we found that the AF-mediated ROS stress could induce PD-L1 expression, revealing an unwanted effect of AF in causing immune suppression. We further showed that a combination of AF with anti-PD-1 antibody could enhance the anticancer activity in a syngeneic immune-competent mouse B-cell lymphoma model. Our study suggests that AF could be a potential drug for lymphoma treatment, and its combination with immune checkpoint inhibitors would be a logical strategy to increase the therapeutic activity.

Accurate determination of gas content in deep coal mine is the premise for the prevention and control of gas disaster, as well as the efficient development and utilization of coalbed methane. Traditional coring technology of coal seam is generally open-ended and requires to obtain the amount of gas loss during the coring process by using estimation methods, making it difficult to ensure the accuracy and validity of the in-situ parameters of coalbed methane. Based on the academic idea of “in-site condition-maintaining coring”, the in-situ pressure- and gas-maintaining coring technology for deep coal seam was proposed, and the in-situ pressure- and gas-maintaining corer for deep coal seam was developed. The strength verification for corer outer tube, pressure-maintaining chamber and key weak parts were conducted based on three-dimensional numerical simulation. Meanwhile, the pressure-maintaining capacity of controller was measured and analysed relied on the self-developed pressure-maintaining coring laboratory simulation test platform, and the reliability of corer was verified through field tests in coal mine. The results shown that, the self-developed corer had the advantages of strong pressure-maintaining capacity, long pressure-maintaining time and stable anti-disturbance performance. The equivalent stress of the corer under internal fluid pressure of 20 MPa and torque of 1000 N·m was 121.1 MPa, which was much smaller than the yield strength of the material and met the strength design requirements. The equivalent stress of corer under the pressure of 20 MPa was 63.9 MPa, which met the strength design requirements. The corer can operate continuously and stably at the pressure of 19.4 MPa, which met the demand for gas testing in most of deep coal mines. The field test shown that, the pressure-maintaining controller of the pressure- and gas-maintaining corer closed well, with 100% of coring rate in the field. The research provides a theoretical, technical and equipment foundation for the accurate determination of gas content in deep coal seams, which is of great significance for the prevention and control of coal mine gas disasters and the exploration and development of coalbed methane.

Introduction It has been a long time since the emergence of COVID-19 in China and its spread worldwide. Vaccines are a promising solution to overcome COVID-19 infection. Still, vaccine hesitancy is the only barrier to controlling the pandemic, as thinking about its side effects induces psychological exhaustion. There is still a lack of research targeting the mental health of Physical Therapists (PTs), especially during the late stage of the COVID-19 pandemic, so the study’s main aim was to identify the prevalence of anxiety, depression and insomnia and identify risk factors that influence anxiety and depression. Methods A cross-sectional online survey via anonymous questionnaires and questions about socio-demographic information was launched on social media in the second half of February 2021. Results Prevalence of anxiety, depression, and insomnia was 42.7%, 50.10%, and 89.30%, respectively, among physical therapists. Common factors that induce anxiety and depression were having children and needing psychological support. Other risk factors for anxiety only were working in a quarantine hospital, being infected with COVID-19, self-isolation after return from the hospital, and hesitance about vaccine effectiveness. Treating COVID-19 patients and having a family member who had died from COVID-19 were risk factors for depression. Consulting a psychiatrist reduced this risk of depression. Conclusions Elevated mental health disturbances triggered the alarm for urgent psychological rehabilitation to prevent the failure of health care systems in this critical period. Additionally, fighting the anti-vaccine movement should be a priority along with enhancing telerehabilitation programs in hospitals.

Abstract Background Plant growth and quality are often affected by environmental factors, including geographical location, climate, and soil. In this study, we describe the effect of altitudinal differences on the growth and active ingredients in Rheum tanguticum Maxim. ex Balf. (R. tanguticum), a traditional Chinese medicinal herb known for its laxative properties. Results The results showed that plants grown at lower altitudes had better growth performances than those in higher altitude areas. The yield varied by 2.45–23.68 times with altitude, reaching a maximum of 102.01 t/ha. In addition, total anthraquinone and total sennoside contents decreased with increasing altitude, whereas total tannins increased with increasing altitude. The total anthraquinone content of the indicator compound reached 5.15% at five experimental sites, which exceeded the Chinese Pharmacopoeia standard by 70.87%. The content of the other two categories of active ingredients reached a maximum value of 0.94% (total sennosides) and 2.65% (total tannins). Redundancy analysis revealed that annual rainfall, annual average temperature, annual sunshine hours, and pH significantly affected growth and active ingredients. Moreover, key metabolites, such as flavonoids, amino acids and their derivatives, phenolic acids, lipids, and terpenes, were differentially expressed between samples from low- and high-altitude cultivation areas. These metabolites were enriched in the flavonoid and flavonol biosynthetic pathway and the monoterpene biosynthetic pathway. Conclusions These results suggest that high anthraquinone content was observed in the lowest-latitude cultivation area due to low rainfall and alkaline soil pH. Key metabolites were significantly upregulated in high-latitude cultivation areas. These results provide a scientific basis for quality control and the systematic cultivation of R. tanguticum.

Anisodus tanguticus (Maxim.) Pascher, a distinctive medicinal plant native to the Qinghai-Tibet Plateau, China, has garnered attention due to increasing market demand. This study explores the impact of environmental factors on the distribution and levels of active compounds namely anisodamine, anisodine, and atropine within A. tanguticus. Our goal was to identify suitable cultivation areas for this plant. This study employs the maximum entropy model to simulate the suitable area of A. tanguticus under current conditions and three climate change scenarios during the 2050s and 2070s. The finding revealed that altitude, precipitation in the warmest season (Bio 18), the average annual temperature (Bio 1) exerted significant influences on the distribution of A. tanguticus. Among the environmental factors considered, temperature difference between day and night (Bio 2) had the most substantial impact on the distribution of anisodamine, temperature seasonal variation variance (Bio 4) predominantly influenced anisodine distribution, and Bio 1 had the greatest effected on the distribution of atropine. The suitable areas primarily exist in the eastern Qinghai-Tibet Plateau in China, encompassing a total area of 30.78 × 104 km2. Under the climate scenarios for the future, the suitable areas exhibit increasing trends of approximately 30.2%, 30.3%, and 39.8% by the 2050s, and 25.1%, 48.8%, and 60.1% by the 2070s. This research would provide theoretical suggestions for the protection, and cultivation management of A. tanguticus resources to face the challenge of global climate change.

Straw return is a promising strategy for managing soil organic carbon (SOC) and improving yield stability. However, the optimal straw return strategy for sustainable crop production in the wheat (Triticum aestivum L.)–cotton (Gossypium hirsutum L.) cropping system remains uncertain. The objective of this study was to quantify the long-term (10 years) impact of carbon (C) input on SOC sequestration, soil aggregation and crop yields in a wheat–cotton cropping system in the Yangtze River Valley, China. Five treatments were arranged with a single-factor randomized design as follows: no straw return (Control), return of wheat straw only (Wt), return of cotton straw only (Ct), return of 50% wheat and 50% cotton straw (Wh-Ch) and return of 100% wheat and 100% cotton straw (Wt-Ct). In comparison to the Control, the SOC content increased by 8.4 to 20.2% under straw return. A significant linear positive correlation between SOC sequestration and C input (1.42–7.19 Mg ha−1 yr−1) (P2 and 1–2 mm at the 0–20 cm soil depth were also significantly elevated under straw return, with the greatest increase of the aggregate stability in the Wt-Ct treatment (28.1%). The average wheat yields increased by 12.4–36.0% and cotton yields increased by 29.4–73.7%, and significantly linear positive correlations were also detected between C input and the yields of wheat and cotton. The average sustainable yield index (SYI) reached a maximum value of 0.69 when the C input was 7.08 Mg ha−1 yr−1, which was close to the maximum value (SYI of 0.69, C input of 7.19 Mg ha−1 yr–1) in the Wt-Ct treatment. Overall, the return of both wheat and cotton straw was the best strategy for improving SOC sequestration, soil aggregation, yields and their sustainability in the wheat–cotton rotation system.

Recently, tracking with unmanned aerial vehicle (UAVs) platforms has played significant roles in Earth observation tasks. However, target occlusion remains a challenging factor during the continuous tracking procedure. In particular, incomplete local appearance features can mislead the tracking network to produce inaccurate size and position estimations when the target is occluded. Furthermore, the tracking network lacks sufficient occlusion supervision information, which may lead to template degradation during template updating. To address these challenges, in this article, we design an occlusion-aware tracker with local-global features modeling, which contains two key components, namely the feature intrinsic association module (FIAM) and the feature verification module (FVM). Specifically, the FIAM divides the local features into blocks and utilizes the transformer network to explore the relative relationships among each subblock, which supplements the damaged local target features and assists the modeling for global target features. In addition, the FVM establishes a correlation measurement network between the target and the template. To precisely evaluate the occlusion status, masked samples with occlusion exceeding 50% are selected as negative samples for independent training, which ensures the purity of the target template. Qualitative and quantitative experiments are conducted on publicly available datasets, including UAV20 L, UAV123, and LaSOT. Qualitative and quantitative experiments have demonstrated the effectiveness of the proposed tracking algorithm over the other state-of-the-art trackers in occlusion scenarios.

Abstract Major depressive disorder (MDD) is predicted to become the second most common cause of disability in the near future. Exposure to glyphosate (Gly)-based herbicides has been linked to the onset of MDD. However, the underlying mechanisms remain unclear. The aim of this study was to investigate the potential molecular mechanisms of MDD induced by Gly using network toxicology approach. The MDD dataset GSE76826 from the Gene Expression Omnibus database was referenced to identify differentially expressed genes (DEGs) in peripheral blood leukocytes of MDD patients and controls. The potential intersection targets of Gly-induced MDD were screened by network toxicology. The intersection targets were used for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and to construct protein–protein interaction networks. The binding potentials of hub targets with Gly were validated by molecular docking. In total, 1216 DEGs associated with Gly-induced MDD were identified. Subsequent network pharmacology further refined the search to 43 targets. GO and KEGG enrichment analyses revealed multiple signaling pathways involved in GLY-induced MDD. Six potential core targets (CD40, FOXO3, FOS, IL6, TP53, and VEGFA) were identified. Finally, molecular docking demonstrated that Gly exhibited strong binding affinity to the core targets. The results of this study identified potential molecular mechanisms underlying Gly induced MDD and provided new insights for prevention and treatment.

Abstract Background Although it is well recognized that core root microorganisms contribute to plant health and productivity, little is known about their role to the accumulation of secondary metabolites. The roots of Anisodus tanguticus, a traditional herbal medication utilized by Tibetan medicine, are rich in tropane alkaloids. We collected wild A. tanguticus populations throughout a 1500 km transect on the Qinghai-Tibetan Plateau. Results Our results showed that despite sampling at a distance of 1500 km, the root of A. tanguticus selectively recruits core root bacteria. We obtained 102 root bacterial core OTUs, and although their number only accounted for 2.99% of the total, their relative abundance accounted for 73% of the total. Spearman correlation and random forest analyses revealed that the composition of core root microbiomes was related to anisodine contents, aboveground biomass and nitrogen contents of Anisodus tanguticus. Among them, the main role is played by Rhizobacter, Variovorax, Polaromonas, and Mycobacterium genus that are significantly enriched in roots. Functional prediction by FAPROTAX showed that nitrogen-cycling microorganisms and pathogenic bacteria are strongly associated with anisodine contents, aboveground biomass and nitrogen contents of Anisodus tanguticus. Conclusions Our findings show that the root selectively recruits core root bacteria and revealed that the core microbiomes and microbial functions potentially contributed to the anisodine contents, aboveground biomass and nitrogen contents of the plant. This work may increase our understanding of the interactions between microorganisms and plants and improve our ability to manage root microbiota to promote sustainable production of herbal medicines.

The Five-hundred-meter Aperture Spherical radio Telescope (FAST) is the world ’ s largest single-dish radio telescope. Its large reflecting surface achieves unprecedented sensitivity but is prone to damage, such as dents and holes, caused by naturally-occurring falling objects. Hence, the timely and accurate detection of surface defects is crucial for FAST’s stable operation. Conventional manual inspection involves human inspectors climbing up and examining the large surface visually, a time-consuming and potentially unreliable process. To accelerate the inspection process and increase its accuracy, this work makes the first step towards automating the inspection of FAST by integrating deep-learning techniques with drone technology. First, a drone flies over the surface along a predetermined route. Since surface defects significantly vary in scale and show high inter-class similarity, directly applying existing deep detectors to detect defects on the drone imagery is highly prone to missing and misidentifying defects. As a remedy, we introduce cross-fusion, a dedicated plug-in operation for deep detectors that enables the adaptive fusion of multi-level features in a point-wise selective fashion, depending on local defect patterns. Consequently, strong semantics and fine-grained details are dynamically fused at different positions to support the accurate detection of defects of various scales and types. Our AI-powered drone-based automated inspection is time-efficient, reliable, and has good accessibility, which guarantees the long-term and stable operation of FAST.

Introduction: Cancer treatments frequently lead to cognitive impairments, affecting a substantial global population. Among various approaches, exercise has emerged as a promising strategy for rehabilitation. However, a comprehensive bibliometric analysis of research in this field is lacking. Methods: We conducted a bibliometric analysis of 10,345 articles sourced from the Web of Science database using the R package “bibliometrix”. Our analysis examined publication trends, leading countries, journals, authors, institutions, keywords, and prevalent themes. Results: Over the past two decades, research on exercise interventions for cancer-related cognitive impairments (CRCI) has advanced significantly. Nonetheless, challenges persist in elucidating underlying mechanisms, developing innovative strategies, and creating effective tools. Conclusions: The number of publications notably increased from 1998 to 2023, although there has been a recent decline in citations. The United States (US) leads in both publications and citations, while China is showing increasing influence. Using Lotka’s Law in our bibliometric analysis, we identified 58 key authors in the field of exercise interventions for CRCI. Leading institutions such as the University of Toronto and Duke University are at the forefront of this research. Although the Journal of Clinical Oncology has fewer publications, it remains influential. Current research focuses on exercise interventions to enhance the quality of life for cancer patients, with particular emphasis on cognitive rehabilitation in breast cancer and the challenges faced by survivors. Future research should delve deeper into intervention mechanisms, behavioral strategies, telemedicine, and precise cognitive assessment tools.

Background: Atrazine (ATR), a commonly used herbicide, is linked to dopaminergic neurotoxicity, which may cause symptoms resembling Parkinson’s disease (PD). This study aims to reveal the molecular regulatory networks responsible for ATR exposure and its effects on dopaminergic neurotoxicity based on an integration strategy. Methods: Our approach involved network toxicology, construction of protein-protein interaction (PPI) networks, gene ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, as well as molecular docking techniques. Subsequently, we validated the predicted results in PC12 cells in vitro. Results: An integrated analysis strategy indicating that 5 hub targets, including mitogen-activated protein kinase 3 (Mapk3), catalase (Cat), heme oxygenase 1 (Hmox1), tumor protein p53 (Tp53), and prostaglandin-endoperoxide synthase 2 (Ptgs2), may play a crucial role in ATR-induced dopaminergic injury. Molecular docking indicated that the 5 hub targets exhibited certain binding activity with ATR. Cell counting kit-8 (CCK8) results illustrated a dose-response relationship in PC12 cells. Real-time quantitative polymerase chain reaction (RT-qPCR) displayed notable changes in the expression of hub targets mRNA levels, with the exception of Mapk3. Western blotting results suggested that ATR treatment in PC12 cells resulted in an upregulation of the Cat, Hmox1, and p-Mapk3 protein expression levels while causing a downregulation in Tp53, Ptgs2, and Mapk3. Conclusion: Our findings indicated that 5 hub targets identified could play a vital role in ATR-induced dopaminergic neurotoxicity in PC12 cells. These results provide preliminary support for further investigation into the molecular mechanism of ATR-induced toxicity.

Abstract Background 46,XY sex reversal 11 (SRXY11) [OMIM#273250] is characterized by genital ambiguity that may range from mild male genital defects to gonadal sex reversal in severe cases. DHX37 is an RNA helicase that has recently been reported as a cause of SRXY11. So far, a total of 21 variants in DHX37 have been reported in 58 cases with 46,XY disorders of sex development (DSD). Methods Whole exome sequencing (WES) was conducted to screen for variations in patients with 46,XY DSD. The subcellular localization of mutant DHX37 proteins was detected by immunofluorescence. And the levels of mutant DHX37 proteins were detected via Western blotting. Results A novel pathogenic variant of DHX37 was identified in a patient with 46,XY DSD c.2012G > C (p.Arg671Thr). Bioinformatics analysis showed that the protein function of the variant was impaired. Compared with the structure of the wild‐type DHX37 protein, the number of hydrogen bonds and interacting amino acids of the variant protein were changed to varying degrees. In vitro assays revealed that the variant had no significant effect on the intracellular localization of the protein but significantly reduced the expression level of the protein. Conclusions Our finding further expands the spectrum of the DHX37 variant and could assist in the molecular diagnosis of 46,XY DSD patients.

Abstract Global warming has raised concerns regarding the potential impact on aquatic biosafety and health. To illuminate the adaptive mechanisms of bdelloid rotifers in response to global warming, the ecological and transcriptomic characteristics of two strains (HX and ZJ) of Rotaria rotatoria were investigated at 25°C and 35°C. Our results showed an obvious genetic divergence between the two geographic populations. Thermal stress significantly reduced the average lifespan of R. rotatoria in both strains, but increased the offspring production in the ZJ strain. Furthermore, the expression levels of genes Hsp70 were significantly upregulated in the HX strain, while GSTo1 and Cu/Zn‐SOD were on the contrary. In the ZJ strain, the expression levels of genes Hsp70, CAT2, and GSTo1 were upregulated under thermal stress. Conversely, a significant decrease in the expression level of the Mn‐SOD gene was observed in the ZJ strain under thermal stress. Transcriptomic profiling analysis revealed a total of 105 and 5288 differentially expressed genes (DEGs) in the HX and ZJ strains under thermal stress, respectively. The PCA results showed clear differences in gene expression pattern between HX and ZJ strains under thermal stress. Interestingly, compared to the HX strain, numerous downregulated DEGs in the ZJ strain were enriched into pathways related to metabolism under thermal stress, suggesting that rotifers from the ZJ strain prioritize resource allocation to reproduction by suppressing costly metabolic processes. This finding is consistent with the life table results. This study provides new insights into the adaptive evolution of aquatic animals in the context of global climate change.

BackgroundIn the previous research, the Disability Assessment Scale based on ICF had been constructed for LTC insurance in China. To apply this scale in further studies, it is essential to establish assessment standards for disability levels.ObjectiveTo establish standardized disability classification criteria and identify the disability statuses and levels in older people.MethodsThis is a cross-sectional study, in which 1,610 older individuals in 15 long-term care institutions in China were assessed by the disability assessment scale based on ICF. Cluster analysis was used for classification of the disability levels. Mean (SD) and median (IQR) were used to describe the scores for each item and each dimension.ResultsThe total scores of the disability assessment scale were classified into six disability levels. The overall disability level of the 1,610 participants was moderate-to-severe. The disability in the dimension of “self-care ability and activity” was the most obvious and severe.ConclusionThe Disability Assessment Scale is capable of identifying disability statuses and levels of older people, and it can serve as a valuable tool for investigating the disabilities among old people and for conducting cross-national comparisons of disability levels.

This study established a residue detection method based on the QuEChERS pre-treatment method and combined it with high-performance liquid chromatography–tandem mass spectrometry to test six herbicides (metamitron, clopyralid, desmedipham, phenmedipham, ethofumesate, and haloxyfop-p-methyl) in sugar beet plants, soil, and roots. The degradation dynamics and terminal residues of each herbicide in sugar beets were analysed. Finally, the dietary risks of various herbicides in sugar beets were evaluated based on the dietary structure of Chinese people, and the risk quotient values were below 100%. Using this detection method, all reagents exhibited good linearity (0.9724 ≤ R2 ≤ 0.9998), The limit of quantification (LOQ) ranged from 0.01 to 0.05 mg/L, the matrix effect ranged from −1.2% to −50%, the addition recovery rate ranged from 77.00% to 103.48%, and the relative standard deviation ranged from 1.61% to 16.17%; therefore, all indicators of this method met the residue detection standards. Under field conditions, the half-lives (t1/2) ranged about 0.65 ∼ 2.96 d and 0.38 ∼ 27.59 d in sugar beet plants and soil, respectively. All herbicides were easily degraded in sugar beet plants and soil (t1/2 < 30 d). The terminal residue amounts in the beet plants, soil, and roots ranged from < LOQ to 0.243 mg/kg. The dietary risk assessment of each pesticide was conducted based on the residual median of the terminal residues and the highest residual values on the edible part of the beetroot. The chronic exposure risk quotient (RQc) and acute exposure risk quotient (RQa) values were < 100%, indicating that the residue of each pesticide in beetroot posed low risks to consumers in China at the recommended dosage.

Occupational exposure to herbicides is an important factor for the development of Parkinson's disease (PD). Atrazine (ATR) and simazine (SIM) are dopamine neurotoxins that can cause PD-like symptoms, but the mechanism is unknown. We analyzed the differentially expressed genes (DEGs) in the substantia nigra of PD patients and controls by using the PD dataset GSE20333 from the Gene Expression Omnibus (GEO) database. The potential intersectional targets of ATR and SIM combined exposure induced PD were screened by network pharmacology. The intersection targets were used for enrichment analysis and to construct protein–protein interaction networks. The binding potentials between hub targets and triazine herbicides were validated using molecular docking, and the cellular exposure models were also constructed to validate the screened results. We identified 934 genes that showed differential expression between individuals with PD and healthy controls. Using network pharmacology, we further refined our search to 151 targets of herbicides known to contribute to PD, and identified eight potential core targets: CDK2, CYCS, FOS, LCK, NTRK2, PDGFRB, PGR, and VEGFA. Finally, molecular docking and molecular biological detection results showed that ATR and SIM each exhibit strong binding affinity to CDK2, CYCS and PGR, both ATR and SIM exposure alone or in combination can affect the expression of core targets. Our study indicates that combined exposure to ATR and SIM may play a critical role in the development of PD and offers a new perspective on occupational prevention and treatment strategies.

With the depletion of shallow resources, the exploration of deep earth resources has become a global strategy. The study of the different patterns in the physical mechanical properties of rocks at different occurrence depths is the basis for exploring deep into the earth, with the core and premise being the acquisition and testing of deep in-situ core specimens. Based on the original idea of deep in-situ condition preserved coring (ICP-Coring) and testing, combined with theoretical modeling, numerical analysis, test platform development, indoor testing and engineering application, the principles and technologies of deep ICP-Coring are developed. This principle and technology consists of five parts: in-situ pressure-preserved coring (IPP-Coring), in-situ substance-preserved coring (ISP-Coring), in-situ temperature-preserved coring (ITP-Coring), in-situ light-preserved coring (ILP-Coring), and in-situ moisture-preserved coring (IMP-Coring). The theory and technology of temperature and pressure reconstruction at different occurrence depths and in different environments are proposed, and prototype trial production was completed by following the principle of displacement and tests based on the in-situ reconstructed environment. The notable advances are as follows: (1) Deep in-situ coring system: A pressure-preserved controller with an ultimate bearing capacity greater than 140 MPa, high-performance (temperature-resistant, pressure-resistant, and low thermally conductive) temperature-preserved materials, an active temperature control system, and high-barrier quality-preserved membrane materials were developed; a deep ICP-Coring capacity calibration platform was independently developed, a deep in-situ coring technology system was developed, and the acquisition of deep in-situ cores was realized. (2) In-situ storage displacement system: Following the dual-circuit hydraulic design idea, a single-drive source push-pull composite grabbing mechanism was designed; the design of the overall structure for the deep in-situ displacement storage system and ultrahigh pressure cabin structure was completed, which could realize docking the coring device and core displacement in the in-situ reconstructed environment. (3) Test analysis system: A noncontact acoustic-electric-magnetic test system was developed under the in-situ reconstructed environment, and the errors between the test results and traditional contact test results were mostly less than 10%; a detachable deep in-situ core true triaxial test system was developed, which could perform loading tests for deep in-situ cores. The relevant technological achievements were successfully applied to the exploration and development of deep resources, such as deep mines, deep-sea natural gas hydrates, and deep oil and gas. The research results provide technical and equipment support for the construction of a theoretical system for deep in-situ rock mechanics, the development of deep earth resources and energy, and the scientific exploration of different layers and occurrence depths (deep and ultradeep) of the Earth.

With the increasing depth of coal mining, the requirements for coring devices that maintain pressure are increasing. To adapt to the special environment in deep coal seams and improve the accuracy of testing gas content, a low-disturbance pressure-preserving corer was developed. The measurement of gas content using this corer was analyzed. The coring test platform was used to complete a coring function test. A pressurized core with a diameter of 50 mm was obtained. The pressure was 0.15 MPa, which was equal to the pressure of the liquid column of the cored layer, indicating that the corer can be successfully used in a mud environment. Next, a pressure test of the corer was conducted. The results showed that under conditions of low pressure (8 MPa) and high pressure (25 MPa), the internal pressure of the corer remained stable for more than 1 h, indicating that the corer has good ability to maintain pressure. Therefore, the corer can be applied at deep coal mine sites. The results of this research can be used to promote the safe exploitation of deep coal mines and the exploitation of methane resources in coalbeds.

Abstract Strongly correlated electronic systems exhibit a wealth of unconventional behavior stemming from strong electron-electron interactions. The LaAlO3/SrTiO3 (LAO/STO) heterostructure supports rich and varied low-temperature transport characteristics including low-density superconductivity, and electron pairing without superconductivity for which the microscopic origins is still not understood. LAO/STO also exhibits inexplicable signatures of electronic nematicity via nonlinear and anomalous Hall effects. Nanoscale control over the conductivity of the LAO/STO interface enables mesoscopic experiments that can probe these effects and address their microscopic origins. Here we report a direct correlation between electron pairing without superconductivity, anomalous Hall effect and electronic nematicity in quasi-1D ballistic nanoscale LAO/STO Hall crosses. The characteristic magnetic field at which the Hall coefficient changes directly coincides with the depairing of non-superconducting pairs showing a strong correlation between the two distinct phenomena. Angle-dependent Hall measurements further reveal an onset of electronic nematicity that again coincides with the electron pairing transition, unveiling a rotational symmetry breaking due to the transition from paired to unpaired phases at the interface. The results presented here highlights the influence of preformed electron pairs on the transport properties of LAO/STO and provide evidence of the elusive pairing “glue” that gives rise to electron pairing in SrTiO3-based systems.

Abstract Background Disulfidptosis is a recently discovered programmed cell death pathway. However, the exact molecular mechanism of disulfidptosis in cutaneous melanoma remains unclear. Methods In this study, clustering analysis was performed using data from public databases to construct a prognostic model, which was subsequently externally validated. The biological functions of the model genes were then investigated through various experimental techniques, including qRT-PCR, Western blotting, CCK-8 assay, wound healing assay, and Transwell assay. Results We constructed a signature using cutaneous melanoma (CM) data, which accurately predicts the overall survival (OS) of patients. The predictive value of this signature for prognosis and immune therapy response was validated using multiple external datasets. High-risk CM subgroups may exhibit decreased survival rates, alterations in the tumor microenvironment (TME), and increased tumor mutation burden. We initially verified the expression levels of five optimum disulfidptosis-related genes (ODRGs) in normal tissues and CM. The expression levels of these genes were further confirmed in HaCaT cells and three melanoma cell lines using qPCR and protein blotting analysis. HLA-DQA1 emerged as the gene with the highest regression coefficient in our risk model, highlighting its role in CM. Mechanistically, HLA-DQA1 demonstrated the ability to suppress CM cell growth, proliferation, and migration. Conclusion In this study, a novel signature related to disulfidptosis was constructed, which accurately predicts the survival rate and treatment sensitivity of CM patients. Additionally, HLA-DQA1 is expected to be a feasible therapeutic target for effective clinical treatment of CM.

The issue of environmental pollution has received considerable critical attention in recent years. It is necessary to develop the green and efficient corrosion inhibitors. In this paper, the inhibition of doxazosin mesylate (DM) and terazosin hydrochloride (TH), as green corrosion inhibitors, were explored systematically by electrochemical impedance, polarization curves, SEM, AFM and contact angle. The effect of different heterocyclic amounts on the corrosion inhibition efficiency for Q235 steel in 1 M HCl was also discussed. It was found that DM showed the better performance than TH, with the best corrosion inhibition efficiency of 96.4 % at 1 mg/L. The FTIR and XPS analysis of the corroded Q235 steel surface revealed that the two drugs formed adsorption films on the steel surface and the types of adsorption were chemical and physical adsorption. Finally, the mechanism of the two drugs was analyzed in detail by isothermal adsorption simulations, quantum chemical calculations and molecular dynamics simulations.

Abstract Patients with colorectal cancer (CRC) and diffuse peritoneal metastasis (PM) are not eligible for surgical intervention. Thus, palliative treatment remains the standard of care in clinical practice. Systemic chemotherapy fails to cause drug accumulation at the lesion sites, while intraperitoneal chemotherapy (IPC) is limited by high clearance rates and associated complications. Given the poor prognosis, a customized OxP/R848@PLEL hydrogel delivery system has been devised to improve the clinical benefit of advanced CRC with diffuse PM. This system is distinguished by its simplicity, security, and efficiency. Specifically, the PLEL hydrogel exhibits excellent injectability and thermosensitivity, enabling the formation of drug depots within the abdominal cavity, rendering it an optimal carrier for IPC. Oxaliplatin (OxP), a first‐line drug for advanced CRC, is cytotoxic and enhances the immunogenicity of tumors by inducing immunogenic cell death. Furthermore, OxP and resiquimod (R848) synergistically enhance the maturation of dendritic cells, promote the expansion of cytotoxic T lymphocytes, and induce the formation of central memory T cells. Moreover, R848 domesticates macrophages to an anti‐tumor phenotype. OxP/R848@PLEL effectively eradicates peritoneal metastases, completely inhibits ascites production, and significantly prolongs mice lifespan. As such, it provides a promising approach to managing diffuse PM in patients with CRC without surgical indications.

Using rice starch as raw material, starch stearate ester (SSE) was prepared by microwave-assisted method, and the SSE and its Pickering emulsion were characterized. The results showed that with the increase of stearic acid (SA) addition, the substitution degree of SSE first increased and then decreased, and the esterification reaction efficiency decreased first, then increased, and then decreased. With the increase of moisture content, the substitution degree and esterification efficiency increased first and then decreased. The effects of hydrochloric acid addition, microwave reaction time, and microwave power on the substitution degree and esterification efficiency were the same as moisture content. X-ray diffraction analysis showed that SSE still had an A-type crystalline structure. The three-phase contact angle of SSE particles increased with the increase of substitution degree. When the substitution degree was 0.0317, the contact angle was 89.6°, and SSE particles showed good oil-water amphiphilicity. The characteristics of Pickering emulsion constructed by SSE as emulsifier were studied, and the emulsification index, particle size and microstructure of the emulsion were analyzed. The results showed that when the addition of SSE was 2.5% and the volume of the oil phase was 60%, the emulsification index of Pickering emulsion was the highest, and the higher the substitution degree of SSE was, the smaller the particle size of the emulsion and the more stable the emulsion were. Particle size analysis and laser confocal microscopy observations showed that SSE could form a stable oil-in-water Pickering emulsion with a narrow droplet size distribution.

Abstract Polarization multispectral imaging (PMI) has been applied widely with the ability of characterizing physicochemical properties of objects. However, traditional PMI relies on scanning each domain, which is time-consuming and occupies vast storage resources. Therefore, it is imperative to develop advanced PMI methods to facilitate real-time and cost-effective applications. In addition, PMI development is inseparable from preliminary simulations based on full-Stokes polarization multispectral images (FSPMI). Whereas, FSPMI measurements are always necessary due to the lack of relevant databases, which is extremely complex and severely limits PMI development. In this paper, we therefore publicize abundant FSPMI with 512 × 512 spatial pixels measured by an established system for 67 stereoscopic objects. In the system, a quarter-wave plate and a linear polarizer are rotated to modulate polarization information, while bandpass filters are switched to modulate spectral information. The required FSPMI are finally calculated from designed 5 polarization modulation and 18 spectral modulation. The publicly available FSPMI database may have the potential to greatly promote PMI development and application.

Abstract Background Cancer survivors face broad challenges in weight loss due to multiple factors. Sarcopenia prevalence among cancer survivors has a wide range and is associated with worse outcomes. Sarcopenia and cancer attract global attention. The use of bibliometrics analysis in this area of interest still needs to be identified. This study was performed to assess the global trends and patterns of sarcopenia and cancer-related scientific publications. Methods Web of Science (WOS) and articles indexed in Science Citation Index Expanded. VOS viewer (Leiden University, Leiden, Netherlands) and R-studio using bibliometrics and R package were used for quantitative analysis of the dataset (year of publications, number of publications, institutes, journals, total citations, H-index status, authors, hotspots of institutes, Keywords, research area, and funding sponsor. Results Our analysis extracted 384 publications from 172 journals written by 2525 authors from the Institute for Web of Science Core Collection database. Overall, 384 articles from the WOS database met the inclusion criteria. The number of published papers has risen since 2014. The results showed that Japan, China and the USA contributed the most to this field. Moreover, our results recognized future research trends and the current condition of sarcopenia and cancer research based on the top 10 most cited articles and the keyword analysis. Finally, the leading author's analysis demonstrated that Shen Xian from Wenzhou Medical University, China, Baracos and Vickie E from the University of Alberta, Switzerland, were the most productive, active, and influential authors. Conclusion Our study demonstrates a comprehensive and objective overview of the up-to-date status of sarcopenia and cancer research. These data would benefit scholars who need information on sarcopenia and cancer research. It would be a reference guide for researchers wanting to conduct additional studies related to the topic.

Resistance of FLT3-ITD acute myeloid leukaemia (AML) patients to FLT3 inhibitors (FLT3i) remains an urgent clinical challenge. Here, the authors identify C/EBPα activation as a mechanism of FLT3i resistance and therapeutically target C/EBPα activation in combination with FLT3i in preclinical models FLT3-ITD AML.

Undercoordinated metal sites with well-defined structures and stability are desired properties for catalysts. Here, the authors construct a class of inorganic-organic hybrid electrocatalysts with enzyme-like properties, which exhibit enhanced electrochemical oxidation of 25 different organics.

Background The development of Alzheimer’s disease (AD) is promoted by a combination of genetic and environmental factors. Notably, combined exposure to triazine herbicides atrazine (ATR), simazine (SIM), and propazine (PRO) may promote the development of AD, but the mechanism is unknown. Aim To study the molecular mechanism of AD induced by triazine herbicides. Methods Differentially expressed genes (DEGs) of AD patients and controls were identified. The intersectional targets of ATR, SIM, and PRO for possible associations with AD were screened through network pharmacology and used for gene ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analysis. The binding potentials between the core targets and herbicides were validated by molecular docking and molecular dynamics. Results A total of 1,062 DEGs were screened between the AD patients and controls, which identified 148 intersectional targets of herbicides causing AD that were screened by network pharmacology analysis. GO and KEGG enrichment analysis revealed that cell cycling and cellular senescence were important signalling pathways. Finally, the core targets EGFR, FN1, and TYMS were screened and validated by molecular docking and molecular dynamics. Conclusion Our results suggest that combined exposure to triazine herbicides might promote the development of AD, thereby providing new insights for the prevention of AD.

The systematic exploration of the application prospects of lotus stem fibers (LSFs) in natural composites is significantly important for the development of green, lightweight, functional biomass materials and products. In this study, the physical and chemical properties, micro/nano structures, micromechanical characteristics, cellulose crystallinity, and thermal stability of LSFs were systematically explored. The density, microstructure, and nanoindentation analyses revealed that the LSF is a lightweight natural fiber with a high modulus (11.2–11.7 GPa) and a good hardness (0.41–0.57 GPa). The transmission electron microscopy analysis indicated the existence of four types of sclerenchyma cells in a gradient distribution in the epidermis and exodermis of the LS. The chemical analysis, X-ray diffraction, and Fourier transform infrared spectroscopy indicated that the outer-layer LSFs have a high cellulose content (44.73%), high relative crystallinity index (49.2%) of cellulose, and small cellulose crystallite size (2.2 nm). As a result, these LSFs possess good mechanical properties.

Background Ulcerative colitis (UC), a chronic inflammatory disease, often cause carcinogenesis, disability, and intestinal perforation. The JingFangFuZiLiZhong formula (JFFZLZ) shows a good effect against UC in the clinic. Hence, we aim to investigate the mechanisms between JFFZLZ and UC via network pharmacology data mining and in vivo experiments.Methods We obtained active constituents and related targets from public databases. The overlapped genes between JFFZLZ and UC targets were further analysed by enrichment analysis. The active constituents and hub targets were used to construct molecule docking analysis. We finally screened out nine hub targets and their expressions were verified in the Gene Expression Omnibus database and UC rats’ colon tissues after JFFZLZ treatment.Results The results implied that JFFZLZ mainly regulated signal transduction, metabolites production, and inflammation pathways. The expression of STAT3, CXCL8, IL6, CXCL12, TNF, TP53, and PTPN11 were both upregulated in colon tissues of UC patients and UC rats. While RELA, EGFR, and TP53 were downregulated in UC patients, but upregulated in UC rats. Furthermore, JFFZLZ could repair UC rats’ colon mucosal damage and promote the healing of ulcers via regulating the hub targets.Conclusion These results elucidated that the anti-UC effect of JFFZLZ was closely related to the inhibition of inflammatory response, inhibition of oxidative stress, and repairing colon mucosal damage through different signal pathways. The findings could contribute to a better understanding of the regulation mechanisms in JFFZLZ against UC.Key messagesJFFZLZ could reduce the inflammatory infiltration and repair UC rats’ colon mucosal damage.Through the network pharmacology-based strategy and public database mining, we obtained the hub targets and key pathways between JFFZLF and UC.The mechanism of JFFZLZ against UC was inhibition of inflammatory response and oxidative stress by regulating the expression of the hub targets.

The benefits of potent antithrombotic therapy usually come at the expense of a higher risk of bleeding. The efficacy and safety of ticagrelor in elderly East Asian populations remains debated due to the concerns about the imbalance of ischemic and bleeding risks. This study aimed to compare the impact of clopidogrel with ticagrelor on clinical outcomes in East Asian patients aged ≥75 years with acute coronary syndrome (ACS) using data from an institutional registry. We assessed the treatment effect of ticagrelor versus clopidogrel based on propensity scores and multivariate Cox proportional hazards models. A total of 2775 ACS patients were included, of which 235 (8.5%) were treated with ticagrelor. The primary efficacy outcome occurred in 11.9% of patients treated with ticagrelor versus 8.8% treated with clopidogrel. There was no significant association between treatment with ticagrelor and a lower risk of the primary efficacy outcome (p = .156). However, the incidences of all-cause death (hazard ratio [HR] 1.69, 95% confidence interval [CI] 1.02 to 2.79) and major bleeding (adjusted HR 2.20, 95% CI 1.06 to 4.56) were significantly higher in patients treated with ticagrelor than clopidogrel. In elderly patients with ACS from East Asia, the efficacy of clopidogrel was comparable to ticagrelor, while ticagrelor is associated with an increased risk of mortality and major bleeding.

It has been known for decades that the incidence of chronic lymphocytic leukemia (CLL) is significantly lower in Asia than in Western countries, but the reason responsible for this difference still remains a major knowledge gap. Using GeneChip® miRNA array to analyze the global microRNA expression in B lymphocytes from Asian and Western CLL patients and healthy individuals, we have identified microRNA with CLL-promoting or suppressive functions that are differentially expressed in Asian and Western individuals. In particular, miR-4485 is upregulated in CLL patients of both ethnic groups, and its expression is significantly lower in Asian healthy individuals. Genetic silencing of miR-4485 in CLL cells suppresses leukemia cell growth, whereas ectopic expression of miR-4485 promotes cell proliferation. Mechanistically, miR-4485 exerts its CLL-promoting activity by inhibiting the expression of TGR5 and activating the ERK1/2 pathway. In contrast, miR-138, miR-181a, miR- 181c, miR-181d, and miR-363 with tumor-suppressive function are highly expressed in Asian healthy individuals. Our study suggests that differential expression of several important microRNA with pro- or anti-CLL functions in Asian and Western B lymphocytes likely contributes to the difference in CLL incidence between the two ethnic groups, and that miR-4485 and its downstream molecule TGR5 could be potential therapeutic targets.

The clinical challenge of bone defects in the craniomaxillofacial region, which can lead to significant physiological dysfunction and psychological distress, persists due to the complex and unique anatomy of craniomaxillofacial bones. These critical-sized defects require the use of bone grafts or substitutes for effective reconstruction. However, current biomaterials and methods have specific limitations in meeting the clinical demands for structural reinforcement, mechanical support, exceptional biological performance, and aesthetically pleasing reconstruction of the facial structure. These drawbacks have led to a growing need for novel materials and technologies. The growing development of 3D printing can offer significant advantages to address these issues, as demonstrated by the fabrication of patient-specific bioactive constructs with controlled structural design for complex bone defects in medical applications using this technology. Poly (ether ether ketone) (PEEK), among a number of materials used, is gaining recognition as a feasible substitute for a customized structure that closely resembles natural bone. It has proven to be an excellent, conformable, and 3D-printable material with the potential to replace traditional autografts and titanium implants. However, its biological inertness poses certain limitations. Therefore, this review summarizes the distinctive features of craniomaxillofacial bones and current methods for bone reconstruction, and then focuses on the increasingly applied 3D printed PEEK constructs in this field and an update on the advanced modifications for improved mechanical properties, biological performance, and antibacterial capacity. Exploring the potential of 3D printed PEEK is expected to lead to more cost-effective, biocompatible, and personalized treatment of craniomaxillofacial bone defects in clinical applications.

IntroductionThe optimal percutaneous coronary intervention (PCI) strategy for coronary left main (LM) bifurcation lesions remains controversial. This meta-analysis compared the medium and long-term follow-up clinical outcomes of single vs. systematic dual stenting strategies of LM bifurcation lesions.MethodsWe systematically identified studies published within 5 years comparing single vs. systematic double stenting strategies for LM bifurcation lesions. The primary endpoint was medium-term (1 year) and long-term (at least 3 years) all-cause death. Secondary outcomes included major adverse cardiovascular events (MACEs), target lesion revascularization (TLR), overall occurrence of stent thrombosis (ST), cardiovascular (CV) mortality, and myocardial infarction (MI).ResultsTwo randomized controlled trials and nine observational studies with 7,318 patients were included in this meta-analysis. In terms of the medium-term follow-up clinical outcomes, our pooled analysis showed that use of the systematic dual stenting strategy was associated with a lower ST risk (odds ratio [OR] = 0.43, 95% confidence interval [CI]: 0.20–0.89, P = 0.02) and cardiac death risk (OR = 0.43, 95% CI: 0.21–0.89, P = 0.02) compared to the single stenting strategy; there was no significant difference between the two strategies regarding rates of all-cause death, MACE, TLR, and MI. Patients with long-term follow-up showed comparable observed clinical outcomes between the two strategies. Most importantly, for patients with true LM bifurcation, the risk of all-cause death, ST, and CV mortality following the systematic dual stenting strategy was significantly lower than the single stenting strategy.ConclusionsFor patients with LM bifurcation lesions, both the systematic dual stenting strategy and single stenting strategy demonstrated comparable results in terms of all-cause mortality during medium-term and long-term follow-up. However, the systematic dual stenting strategy showed a tendency towards lower incidence of ST and CV mortality compared to the single stenting strategy during medium-term follow-up. Consequently, the systematic dual stenting strategy yielded superior clinical outcomes for patients with LM bifurcation lesions.

Background: Breast invasive carcinoma (BRCA) is a malignant tumor with high morbidity and mortality, and the prognosis is still unsatisfactory. Both ferroptosis and cuproptosis are apoptosis-independent cell deaths caused by the imbalance of corresponding metal components in cells and can affect the proliferation rate of cancer cells. The aim in this study was to develop a prognostic model of cuproptosis/ferroptosis-related genes (CFRGs) to predict survival in BRCA patients.Methods: Transcriptomic and clinical data for breast cancer patients were obtained from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Cuproptosis and ferroptosis scores were determined for the BRCA samples from the TCGA cohort using Gene Set Variation Analysis (GSVA), followed by weighted gene coexpression network analysis (WGCNA) to screen out the CFRGs. The intersection of the differentially expressed genes grouped by high and low was determined using X-tile. Univariate Cox regression and least absolute shrinkage and selection operator (LASSO) were used in the TGCA cohort to identify the CFRG-related signature. In addition, the relationship between risk scores and immune infiltration levels was investigated using various algorithms, and model genes were analyzed in terms of single-cell sequencing. Finally, the expression of the signature genes was validated with quantitative real-time PCR (qRT‒PCR) and immunohistochemistry (IHC).Results: A total of 5 CFRGs (ANKRD52, HOXC10, KNOP1, SGPP1, TRIM45) were identified and were used to construct proportional hazards regression models. The high-risk groups in the training and validation sets had significantly worse survival rates. Tumor mutational burden (TMB) was positively correlated with the risk score. Conversely, Tumor Immune Dysfunction and Exclusion (TIDE) and tumor purity were inversely associated with risk scores. In addition, the infiltration degree of antitumor immune cells and the expression of immune checkpoints were lower in the high-risk group. In addition, risk scores and mTOR, Hif-1, ErbB, MAPK, PI3K/AKT, TGF-β and other pathway signals were correlated with progression.Conclusion: We can accurately predict the survival of patients through the constructed CFRG-related prognostic model. In addition, we can also predict patient immunotherapy and immune cell infiltration.

Abstract Endometriosis is a common, estrogen‐dependent chronic gynecological disease that endangers the reproductive system and systemic metabolism of patients. We aimed to investigate the differences in metabolic profiles in the follicular fluid between infertile patients with endometriosis and controls. A total of 25 infertile patients with endometriosis and 25 infertile controls who were similar in age, BMI, fertilization method and ovulation induction treatment were recruited in this study. Metabolomics analysis of follicular fluid was performed by two methods of high‐performance liquid chromatography tandem mass spectrometry. There were 36 upregulated and 17 downregulated metabolites in the follicular fluid of patients in the endometriosis group. KEGG pathway analysis revealed that these metabolites were enriched in phenylalanine, tyrosine and tryptophan biosynthesis, aminoacyl‐tRNA biosynthesis, phenylalanine metabolism and pyrimidine metabolism pathways. A biomarker panel consisting of 20 metabolites was constructed by random forest, with an accuracy of 0.946 and an AUC of 0.988. This study characterizes differences in follicular fluid metabolites and associated pathway profiles in infertile patients with endometriosis. These findings can provide a better comprehensive understanding of the disease and a new direction for the study of oocyte quality, as well as potential metabolic markers for the prognosis of endometriosis.

We present observations using the Northern Extended Millimetre Array (NOEMA) of CO and H _2 O emission lines and the underlying dust continuum in two quasars at z ∼ 6, i.e., P215−16 at z = 5.78 and J1429+5447 at z = 6.18. Notably, among all published CO spectral line energy distributions (SLEDs) of quasars at z ∼ 6, the two systems reveal the highest and the lowest CO levels of excitation, respectively. Our radiative transfer modeling of the CO SLED of P215−16 suggests that the molecular gas heated by active galactic nuclei could be a plausible origin for the high CO excitation. For J1429+5447, we obtain the first well-sampled CO SLED (from transitions from 2−1 to 10−9) of a radio-loud quasar at z ≳ 6. Analysis of the CO SLED suggests that a component from a single photodissociation region could explain the CO excitation in the radio-loud quasar J1429+5447. This work highlights the utility of the CO SLED in uncovering the properties of the interstellar medium in these young quasar–starburst systems at the highest redshift. The diversity of the CO SLEDs reveals the complexities in gas conditions and excitation mechanisms at their early evolutionary stage.

Polarized holographic properties play an important role in the holographic data storage of traditional organic recording materials. In this study, reduced graphene oxide (RGO) was introduced into a phenanthraquinone-doped polymethylmethacrylate (PQ/PMMA) photopolymer to effectively improve the orthogonal polarization holographic properties of the material. Importantly, the lateral size of RGO nanosheets has an important influence on the polymerization of MMA monomers. To some extent, a larger RGO diameter is more conducive to promoting the polymerization of MMA monomers and can induce more PMMA polymers to be grafted on its surface, thus obtaining a higher PMMA molecular weight. However, too large of a RGO will lead to too much grafting of the PMMA chain to shorten the length of a single PMMA chain, which will lead to the degradation of PQ/PMMA holographic performance. Compared with the original PQ/PMMA, the diffraction efficiency of the RGO-doped PQ/PMMA photopolymer can reach more than 11.4% (more than 3.5 times higher than the original PQ/PMMA), and its photosensitivity is significantly improved by 4.6 times. This study successfully synthesized RGO-doped PQ/PMMA high-performance photopolymer functional materials for multi-dimensional holographic storage by introducing RGO nanoparticles. Furthermore, the polarization holographic properties of PQ/PMMA photopolymer materials can be further accurately improved to a new level.

Using data from AidData and the conflict report from Uppsala Conflict Data Program (UCDP) from 2000 to 2014, we find that China's other official flows (OOF) aid can significantly reduce the frequency of conflicts. To deal with the endogeneity, we use China's competitive capacity as the instrumental variable. Our results are robust when considering the possible sample selection bias and different types of international aid. We find that increasing the local infrastructure investment and employment is the main mechanism, which increases the opportunity cost of engaging in conflicts. Our results provide evidence on the effectiveness of China's aid mode and the importance of infrastructure investment in developing countries.

The world’s rapid industrialisation and population expansion have led to water pollution, causing significant disruption to the activities of humans, animals, and plants. Organic contamination content in water is commonly evaluated by measuring the chemical oxygen demand (COD). However, traditional COD detection methods often require additional reagents, resulting in secondary contamination and extended detection time. In this study, we propose and implement a reflective detection system that measures the UV-Vis absorption spectra of COD in water without contact measurement. We compared the modeling results of the transmissive and reflective detection systems using three regression analysis algorithms. We also assessed the modeling results using various spectral preprocessing and different feature selection bands. The results of the standard samples confirmed the viability of the reflective detection system for detecting COD, with the impressive coefficient of determination (R2) of 0.98892, the root mean square error (RMSE) of 2.86776, and the detection time of only 47.6 s. For the transmissive detection system, the R2 was 0.99976, the RMSE was 0.41979, and the detection time was 162.4 s. Overall, this study proposes two referenceable detection methods for measuring COD concentrations, which can be adapted to suit various job demands.

At present, the development of efficient green corrosion inhibitors has become one of the important directions of metal corrosion protection. In this paper, biomass carbon quantum dots (BCQDs) were prepared by hydrothermal reaction using biomass precursors. The optical properties and structural composition of the synthesized BCQDs were characterized in detail. After that, the corrosion inhibition performance of BCQDs was evaluated by EIS, Tafel, SEM and TEM. The electrochemistry and surface tests demonstrate that the aggregates can effectively prevent Q235 steel from corrosion in 1 M HCl medium at 298 K-328 K, with the maximum inhibition efficiency of 94.1 % at the concentration of 200 mg/L at 308 K. Finally, the corrosion inhibition mechanism was analyzed by FTIR, CA and XPS on the surface of the corroded carbon steel samples. The inhibition mechanism suggests that BCQDs can inhibit metal corrosion by self-aggregation and adsorption on metal surface. This is owing to its small size effect and functional groups containing heteroatoms, easily forming dense protective film.

Atrazine (ATR) is a widely applied herbicide in Asia and South America with slow natural degradation and documented deleterious effects on human and animal health, including hippocampal toxicity. However, relatively little is known about the molecular mechanisms responsible for ATR-induced hippocampal damage. Screening for differentially expressed mRNAs and microRNAs (miRNAs), and construction of potential miRNA-mRNA regulatory networks can reveal such mechanisms, so we analyzed the mRNA and miRNA expression profiles of rat hippocampus-derived H19–7 cells in response to ATR (500 μM) and conducted Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes enrichment (KEGG) analyses. Integration of miRNA sequencing (miRNA-seq) and mRNA sequencing (mRNA-seq) results identified 114 differentially expressed miRNAs (DEMIs, 40 upregulated and 74 downregulated), and 510 differentially expressed mRNAs (DEMs, 177 upregulated and 333 downregulated) targeted by these DEMIs. The top 10 hub mRNAs (Fos, Prkcb, Ncf1, Vcam1, Atf3, Pak3, Pak1, Cacna1s, Junb, and Ccl2) and 19 related miRNAs (rno-miR-194–5p, rno-miR-24–3p, rno-miR-3074, rno-miR-1949, rno-miR-218a-1–3p, rno-miR-1843a-5p, rno-miR-1843b-5p, rno-miR-296–3p, rno-miR-320–3p, rno-miR-219a-1–3p, rno-miR-122–5p, rno-miR-1839–5p, rno-miR-1843a-3p, rno-miR-215, rno-miR-3583–3p, rno-miR-194–3p, rno-miR-128–1–5p, rno-miR-1956–5p, and rno-miR-466b-2–3p) were validated by quantitative real-time PCR. GO analysis indicated that these DEMs were enriched in genes associated with synaptic plasticity and antioxidant capacity, while KEGG analysis suggested that enriched DEMs were involved in calcium signaling, axon guidance, MAPK signaling, and glial carcinogenesis. The miRNA-mRNA regulatory network identified here may provide potential biomarkers and novel strategies for the treatment of hippocampal neurotoxicity induced by ATR.