Search

To elucidate the mechanism of succinic acid (SA) inhibition of Microcystis aeruginosa, the chlorophyll fluorescence transients, photosynthesis, photosynthetic electron transport activity, and gene expression of M. aeruginosa were evaluated under various doses of SA. The results demonstrated that, after treatment with 60 mg L-1 SA for 1 h, the chlorophyll fluorescence transients and related parameters changed significantly, indicating that the function and structure of photosynthetic apparatuses of Microcystis were seriously damaged. The initial quantum efficiency α, maximum net photosynthetic rate Pnmax, dark respiration rate Rd, and gross photosynthetic rate decreased to 57%, 49%, 49%, and 46%, respectively, relative to the control. Furthermore, photosystem II (PSII) activity (H2O→p-BQ) and the electron transport activity of H2O→MV and DPC→MV significantly decreased. Real-time PCR analysis revealed that, following incubation with 60 mg L-1 SA for 24 h, the expression level of core protein genes (psbA, psaB, psbD, and psbO) of the photosynthesis centers photosystem I (PSI) and PSII decreased significantly. However, the transcription of gene nblA encoding phycobilisome degradation protein was elevated. The downregulation of the rbcL gene, which encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), resulted in the suppression of CO2 fixation and assimilation. High concentration (60 mg L-1) of SA resulted in damage to oxygen-evolving complex (OEC) and reaction center of PSII, blocking photosynthetic electron transport, thereby lowering the rate photosynthesis and inhibiting the growth of Microcystis. We concluded that inhibition of photosynthesis is an important mechanism of SA inhibition in M. aeruginosa.

Pontederia cordata is a heavy metal accumulator, while the heavy metal tolerance mechanisms of this plant are not well understood. Hydroponic experiments were used to assess the effects of Cd2+ on antioxidative activities, osmoregulatory substances and photosynthesis in leaves. Exposure of 5 mg L−1 Cd2+ for 7 days, the photosynthetic apparatus functioned normally and sustained a relatively high photosynthetic rate, and good growth was observed. Under 50 and 75 mg L−1 Cd2+, accelerated lipid peroxidation and increased peroxidase activity (POD; E.C.1.11.1.7) were detected, while no significant differences were observed in superoxide dismutase (SOD; E.C.1.15.1.1) and catalase (CAT; E.C.1.11.1.6) activities, as well as in lutein, ascorbic acid, and glutathione contains of leaves. Proline content increased, while soluble sugar and soluble protein contents decreased under 75 mg L−1 Cd2+. Cd2+ at different concentrations induced a reduction in carotenoid, total carotenoid, and ascorbic acid-dehydroascorbate contents. A significant increase in phytochelatin content was induced by 75 mg L−1. Chlorophyll content decreased under Cd stress and disturbed photosynthesis, causing dramatic reductions in photosynthetic parameters. Stomatal closure was responsible for a reduced photosynthetic rate under Cd2+ exposure. Cd2+ concentrations of no less than 25 mg L−1 disorganized the photosynthetic apparatus, induced the partial closure, and decreased activity of the photosystem II (PS II) reaction center, thus disturbing light conversion and utilization, thereby decreasing the photosynthetic efficiency in PS II.

Pontederia cordata can tolerate heavy metal toxicity and possesses great potential for phytoremediation of heavy-metal-contaminated wetlands, yet how it copes with heavy metal stress has still not been determined. Hydroponic experiments were used to assess the effects of various levels of Cd2+ on the photosynthesis and activity of redox-regulatory systems in the plant leaves, and we also sought to elucidate the tolerance mechanism of the plant to Cd2+ by investigating Cd2+ enrichment characteristics and chemical forms. The plant can manage a low cadmium concentration (≤0.04 mM) with relatively stable biomass and photosynthetic performance. Cd2+ at the highest concentration (0.44 mM) decreased superoxide dismutase and peroxidase activities by 37.17% and 93.29%, respectively. Similar trends were demonstrated in the contents of ascorbic acid, carotenoids, lutein, glutathione, and non-protein thiol, as well as phytochelation in the leaves, exacerbating membrane peroxidation despite the significantly increased catalase activity observed. Moreover, the highest Cd2+ concentration disturbed the biosynthesis of chlorophyll precursors in the leaves, reduced chlorophyll a and b, as well as total chlorophyll contents by 60.47%, 67.47%, and 68.12%, respectively, which inhibited photosynthesis, leading to a decline in biomass. Compared with maximum quantum efficiency (Fv/Fm) and the potential activity (Fv/Fo) of photosystem II, the performance index for energy conservation from photons absorbed by PSII to the reduction of intersystem electron acceptors (PIabs), and of PSI end acceptors (PItotal), can indicate Cd2+ toxicity to the photosynthetic apparatus in the leaves. 49.95%–76.90% of the Cd2+ was sequestered in the plant roots, restraining translocation from roots to shoots, which is considered a tolerance mechanism, probably resulting from disturbed transpiration in leaves and increased Cd2+ content with low activity. Pontederia cordata is a candidate plant for phytoremediation of heavy-metal -contaminated wetlands.

Two solution cultures with different oxygen pretreatments were used to investigate (ⅰ) the variation in the radial oxygen loss in the roots and root morphology of Triarrhena sacchariflora seedlings and (ii) their tolerance to Cu2+ and Cd2+, as well as both the metal uptake and accumulation by pretreated seedlings. Developed aerenchyma in the roots was induced by the hypoxia pretreatment (HP) and aeration pretreatment (AP), for which root porosity, respectively, increased by 45.76%–53.39% and 84.07%–88.66%. AP altered the natural radial oxygen loss coupled to an enhanced secretion of oxygen in the root tips. AP was found to effectively improve the seedlings’ tolerance to Cu2+ and Cd2+, facilitating their growth, thereby increasing their root diameter, dry weight, and number of root tips, as well as promoting shoot growth. AP was capable of promoting the uptake and bioaccumulation in seedlings of Cu2+ and Cd2+; it also induced more Cu2+ and Cd2+ immobilized in roots so that less of either metal was transported from roots to shoots, which may well be a key mechanism for strengthening seedlings’ tolerance to metal ions. Our experimental results suggest that AP offers great potential for the remediation of heavy metal-contaminated wetlands.

Hydroponic experiments were conducted to assess the accumulation, translocation, and chemical forms of lead (Pb) and cadmium (Cd) in the roots, stems, and leaves of Triarrhena sacchariflora seedlings and the associated variation in leaf ultrastructure. The leaves and leaf ultrastructure showed no significant symptoms of toxicity with 0.05 mM Pb or 0.01 mM Cd exposure for 10d. Chlorosis and wilting were observed in leaves when the Pb and Cd concentration was higher than 0.1 and 0.05 mM in the medium, respectively, as demonstrated by severe ultrastructural modifications at higher concentration in the leaves, such as plasmolysis, cell wall detachment, chloroplast swelling, nuclear condensation, and even nuclear fragmentation. The Pb and Cd concentrations in the roots was significantly higher than those in the stems and leaves. This indicated low Pb and Cd translocation from the roots to the aboveground parts. Subcellular distribution analysis showed that the majority of Pb and Cd was bound to the cell wall, especially in the roots, indicating that the cell wall likely constitutes a crucial storage site for Pb and Cd. This mechanism decreases the translocation of Pb and Cd across membranes and is more effective than vacuolar compartmentation. The majority of Pb and Cd exited in form of insoluble Pb/Cd-pectate or -oxalate complexes in the plant. In conclusion, higher concentrations of Pb or Cd induced premature senescence. High Pb and Cd enrichment was observed in the roots, which decreased the translocation of Pb and Cd from the roots to the aboveground tissues. The immobilization of Pb or Cd by the cell wall is important for plant detoxification and can protect protoplasts from Pb or Cd toxicity. Pb and Cd mainly existed in insoluble Pb/Cd-phosphate or -oxalate complexes, exhibiting low activity and thereby limiting symplastic transport and suppressing toxicity.