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ObjectiveInvestigating the mental health status of Chinese resident physicians during the 2019 new coronavirus outbreak.MethodsA cluster sampling method was adopted to collect all China-wide resident physicians during the epidemic period as the research subjects. The Symptom Checklist-90 self-rating scale was used to assess mental health using WeChat electronic questionnaires.ResultsIn total, 511 electronic questionnaires were recovered, all of which were valid. The negative psychological detection rate was 93.9% (480/511). Among the symptoms on the self-rating scale, more than half of the Chinese resident physicians had mild to moderate symptoms of mental unhealthiness, and a few had asymptomatic or severe unhealthy mental states. In particular, the detection rate of abnormality was 88.3% (451/511), obsessive-compulsive symptoms was 90.4% (462/511), the sensitive interpersonal relationship was 90.6% (463/511), depression abnormality was 90.8% (464)/511), anxiety abnormality was 88.3% (451/511), hostility abnormality was 85.3% (436/511), terror abnormality was 84.9% (434/511), paranoia abnormality was 86.9% (444/511), psychotic abnormalities was 89.0% (455/511), and abnormal sleeping and eating status was 90.8% (464/511). The scores of various psychological symptoms of pediatric resident physicians were significantly lower than those of non-pediatrics (p < 0.05).ConclusionThe new coronavirus epidemic has a greater impact on the mental health of Chinese resident physicians.

As a pivotal regulator of 5’ splice site recognition, the U1 small nuclear ribonucleoprotein (U1 snRNP)-specific protein C (U1C) regulates pre-mRNA splicing by interacting with other components of U1 snRNP complex. Previous studies have shown that U1 snRNP and its components are linked to variety of diseases including cancer. However, phylogenetic relationship and expression profiles of U1C have not been studied systematically. To this end, we identified a total of 110 animal U1C genes and compared to the homologs from yeast and plants. Bioinformatics analysis shows that the structure and function of U1C proteins is relatively conserved, and is found in multiple copies in few members of U1C genes family. Furthermore, expression pattern showed that U1Cs have potential role in cancer progression and human development. In summary, our study presents a comprehensive overview of animal U1C gene family, which can provide fundamental data and potential cues for further research in deciphering the molecular function of this splicing regulator.

Long-term hyperoxia exposure may cause lung damage with characteristic inflammation. Long noncoding RNA of maternally expressed 3 (MEG3) is up-regulated in lung tissues exposed to hyperoxia; however, the underlying mechanism is unclear. Hyperoxia-induced cells and mouse models were used to study these mechanisms. Molecular assays were used to detect cell viability, cytotoxicity, and expression of miR-18a, MEG3, and inflammatory cytokines. The interaction among MEG3, miR-18a, and thioredoxin-interacting protein (TXNIP) was verified; and pyroptosis-related proteins were analyzed. The in vivo model was established by exposing MEG3 knockdown mice to hyperoxia. Hematoxylin and eosin staining was used to assess pathologic alterations of lung tissues. Hyperoxia suppressed cell viability, induced cell damage, and exacerbated the secretion of IL-1β and IL-18. Hyperoxia inhibited miR-18a, with increased expression of MEG3, TXNIP, and nonobese diabetic–like receptor family pyrin domain containing 3 (NLRP3). MEG3 aggravated TXNIP expression by binding to miR-18a. Knockdown of MEG3 rescued hyperoxia-induced pyroptosis by up-regulating miR-18a. Furthermore, knockdown of MEG3 inhibited NLRP3 inflammasome activity and caspase-1 signaling by miR-18a. In vivo knockdown of MEG3 and overexpression of miR-18a relieved hyperoxia-induced lung injury via restraining NLRP3 inflammasome-mediated pyroptosis, whereas miR-18a inhibition reversed these effects. In conclusion, knockdown of MEG3 inhibits pyroptosis to alleviate hyperoxia lung injury by suppressing NLRP3 inflammasome and caspase-1 signaling via regulating miR-18a–TXNIP axis.

Twelve new diterpenes, caryopincaolide A-L (1-4, 11-12, 16-19, 27-28), together with twenty-eight known diterpenes, have been isolated from the whole plant of Caryopteris incana (Thunb.) Miq. Their structures were elucidated on the basis of 1D and 2D NMR, IR, X-ray crystal diffraction and mass spectroscopic data, as well as ECD calculations. All compounds were tested for in vitro dipeptidyl peptidase IV (DPP-IV) inhibitory activity, with compounds 3, 4, 28, 29, and 40 exhibiting DPP-IV inhibitory effects with IC50 values ranging from 54.2 to 228.9 μM. Compounds 1, 3 and 4 also showed potent activity toward the inhibition of the growth of human cancer cells and 1 can induce apoptosis in Hey and A-549 cells.

Cadmium (Cd) contamination in paddy soils and the related pollution risk of rice grain have received increasing attention. Agronomic measures, such as the application of sulfur and changes in water regimes, were reported to mitigate the accumulation of Cd in rice. However, there is limited information on the combined effects of sulfur application and water regimes. Therefore, a pot experiment was conducted to investigate the effects of two sulfur forms, three water regimes and multiple sulfur application rates on Cd accumulation in rice. The sulfur was applied as SO42− (SVI, replacing the traditional fertilizers by SO42--containing fertilizers), and element S (S0) was applied at 0, 10, 20, 30 and 40 mg S kg−1 soil. The water regimes were continuous flooding (F), flooding-moist alternation (FM), and moist irrigation (M), for a total of 30 treatments. The results indicated that application of SVI exceeding 30 mg S kg−1 significantly reduced the Cd concentrations in brown rice by 31.1–56.3%, and the Cd concentrations decreased with increasing amount of irrigation water. Similar reductions in Cd concentrations in rice shoots and rice straw collected at tillering and maturity stages were observed after application of SVI. However, the effect of S0 application on Cd accumulation in grain was not significant under different water regimes. Furthermore, this study found that application of both SVI and S0 inhibited the transfer of Cd from rice roots to shoots in most cases. These findings indicate that replacing traditional fertilizers with SO42--containing fertilizers, especially combined with increased irrigation, could be a potential approach to mitigate Cd accumulation in rice growing in Cd-contaminated acidic paddy soils.

With the improvement of living standard, gestational diabetes mellitus (GDM) incidence is increasing every year. We observed the effects of abnormal 75 g oral glucose tolerance test (OGTT) at different time points on neonatal complications and neurobehavioral development in GDM. A total of 144 newborns whose mothers were diagnosed with GDM and received prenatal examination and childbirth in our hospital from October 2015 to April 2016, were observed in this study. Pregnant women underwent 75 g OGTT and the blood glucose level was recorded on an empty stomach, as well as postprandial 1 and 2 hours, respectively. Based on the frequency of 75 g OGTT-abnormal time points, the pregnant women were divided into group 1 (OGTT abnormality at 1 time point), group 2 (OGTT abnormality at 2 time points), and group 3 (OGTT abnormality at 3 time points). Neonatal behavioral neurological assessment (NBNA) was performed on the 3 groups, respectively. In the total score of NBNA, there was a significant difference among the 3 groups (F = 17.120, P = .000), and there were significant differences between the 3 groups (all P

Nepetaefolins A–J (1–10) and seven known compounds were isolated from the whole plant of Caryopteris nepetaefolia. The absolute configurations of 1–3 were determined from single-crystal X-ray diffraction and spectroscopic data. Compounds 6 and 7, with IC50 values of 6.3–9.0 μM, showed higher cytotoxicity than paclitaxel in one non-small-cell lung cancer, patient-derived xenograft (PDX) model when tested using PDX models and the adenosine triphosphate-tumor chemosensitivity assay (ATP-TCA).

Twelve new diterpenes, caryopincaolide A-L (1-4, 11-12, 16-19, 27-28), together with twenty-eight known diterpenes, have been isolated from the whole plant of Caryopteris incana (Thunb.) Miq. Their structures were elucidated on the basis of 1D and 2D NMR, IR, X-ray crystal diffraction and mass spectroscopic data, as well as ECD calculations. All compounds were tested for in vitro dipeptidyl peptidase IV (DPP-IV) inhibitory activity, with compounds 3, 4, 28, 29, and 40 exhibiting DPP-IV inhibitory effects with IC50 values ranging from 54.2 to 228.9 μM. Compounds 1, 3 and 4 also showed potent activity toward the inhibition of the growth of human cancer cells and 1 can induce apoptosis in Hey and A-549 cells.

A field experiment was conducted to investigate the effects of soil amendments (lime, nano-Si foliar solution and used diatomite) on the growth and metal uptake of three maize ( Zea mays L.) cultivars grown in a Cd and Zn-contaminated acidic soil. The addition of lime significantly increased the maize grain yields and decreased the concentrations of Zn and Cd in the grains and shoots of maize when compared with the control. Among the three maize cultivars, Yunshi-5 accumulated the lowest amounts of Cd and Zn in the grain. The concentrations of Zn and Cd in the grain of Yunshi-5 conformed to the Chinese feed standards. These data revealed that a combination of low metal-accumulating maize and chemical fixation could effectively provide a barrier to prevent metals from entering the human food chain.

To conduct preliminary investigation to the species and reserves of medicinal plants in Huangfu Mountain, and to provide references to the general survey of those plants for medicine.Combined with global positioning system (GPS), the program of investigation with grid sampling was used in this resource survey of medicinal plants.After the preliminary investigation of the plants for medical use of Huangfu Mountain, it is found that there are 103 families with 313 kinds of plants. There are many medicinal plants and large distribution, such as Pseudostellaria heterophylla, Semiaguilegia adoxoides and Pinellia ternate.Huangfu Mount, with so many different kinds of medicinal plants and comfortable environment for part of the medicinal plants to grow, could be developed as a base for planting Chinese herbal medicines.

To comprehend the connections and differences of the three sources of Siegesbeckiae Herba.Using traditional Chinese medicine micro-macroscopical identification to identify these three sources of Siegesbeckiae Herba.Three sources of Siegesbeckiae Herba are obviously different when using micro-macroscopical identification.Micro-macroscopical identification can distinguish three sources of Siegesbeckiae Herba veritably and directly.

To study the influence of mixed chelators (MC) on Zn leaching in two contaminated soil (acid soil with pH 4.63 and neutral soil with pH 6.51) with Pb-Zn mining wastewater under the co-planting system, a outdoor soil column (100 cm) leaching experiment was conducted with three treatments as follows: without plants and without MC (CK), co-planting of S. alfredii and Z. mays without MC (CP), and co-planting amended with MC (CPMC). Field conditions of the soil, including bulk density and profile, were reproduced in the soil columns. The concentration of Zn in leachates at 20, 40, 60, 80 and 100cm depth of soil column was measured on day 13 and day 6 before application of MC and on day 2 and day 8 after application, respectively. The soil pH and Zn concentration at each depth was measured before and after the experiment. The results showed that on day 2 after application of MC, the Zn concentration in leachates at each depth of both neutral and acid soil increased significantly. On day 8, the concentration of Zn in leachates at each depth of neutral soil and at 80 and 100 cm depth of acid soil was still significantly higher. However, the mobility of Zn on day 8 decreased substantially compared with that on day 2. The DTPA-extractable Zn concentration in both acid and neutral soils showed no significant differences among the CP, CPMC, and CK treatments. The DTPA-extractable Zn in acid soil and the total Zn in both soils showed a trend of downward migration. The results also indicated that in Zn-contaminated soil, the addition of MC might enhance the potential risks of greater Zn contamination in groundwater.

In order to elucidate the influence of chelators on Cd leaching in contaminated soil, outdoor soil column (100 cm) leaching experiments were conducted using two paddy soils irrigated with Pb-Zn mining wastewater. Soil samples which under intercropping systems were collected from Qingyuan City (acid soil with pH 4.63) and Lechang city (neutral soil with pH 6.51), Guangdong Province of China. The mixture of chelators (MC) comprised of citric acid, monosodium glutamate waste liquid, EDTA and KCl with molar ratio of 10 : 1 : 2 : 3 at the concentration of 5 mmol x kg(-1) soil. The intercropping system used in this study was a Zn- and Cd-hyperaccumulator (Sedum alfredii) and a low-accumulating crop (Zea mays). Results showed that at day 2 after the application of MC, the Cd concentrations in leachates from every layer of neutral and acid soils increased significantly in the treatment with intercropping and MC. At day 8 the concentrations of Cd in leachate from layers below 20 cm in the neutral soil and below 60 cm in the acid soil were still significantly higher than those of control. However, the mobility of Cd was decreased greatly compared with that at day 2. At day 2 and day 8 the Cd concentrations in leachates from every layer of neutral and acid soils in the Co-crop + MC treatments exceed the value of the Groundwater Quality Standards (GB/T 14848-93). Cd in all soil columns showed the trend to migrate downwards, especially in the acid soil. The total Cd in the soil layers of 20 cm and 40 cm was decreased by 40% -58% and 39%-49% respectively at the end of the experiments compared to the initial value. After leaching of 100 days,the total Cd in 0-40 cm soil layer of acid soil reached the limit of National Soil Environmental Quality Standards (GB 15618-1995). The results also implied that in Cd-contaminated soil MC addition might enhance the potential risks of Cd contamination in groundwater.

To study the characters and etiology of oral ulcer and to propose the ways of prevention.Summarize the 52 cases of oral ulcer in the earthquake area were summarized.The disease was related to the psychological tension, living regular and bad conditions. When the comprehensive therapies finished, the clinical curative rate was 82.7%(43/52), the efficacy was 15.4%(8/52), only one case was ineffective.Those patients who were diagnosed oral ulcer in the earthquake area need comprehensive therapies, including getting rid of the intensive emotion, improving the living conditions and paying attention to rest.

To provide basis for making the quality standard of medicinal chrysanthemums [Dendranthema morifolium (Ramat.) Tzvel.].The character of pharmacognosy.The characters of many of chrysanthemums as well as the differences between them have been clarified.

AIM:To further study the properties of bile liquid crystals, and probe into the relationship between bile liquid crystals and gallbladder stone formation, and provide evidence for the prevention and treatment of cholecystolithiasis.METHODS:The optic properties of bile liquid crystals in human body were determined by the method of crystal optics under polarizing microscope with plane polarized light and perpendicular polarized light.RESULTS:Under a polarizing microscope with plane polarized light, bile liquid crystals scattered in bile appeared round, oval or irregularly round. The color of bile liquid crystals was a little lighter than that of the bile around. When the stage was turned round, the color of bile liquid crystals or the darkness and lightness of the color did not change obviously. On the border between bile liquid crystals and the bile around, brighter Becke-Line could be observed. When the microscope tube is lifted, Becke-Line moved inward, and when lowered, Becke-Line moved outward. Under a perpendicular polarized light, bile liquid crystals showd some special interference patterns, called Malta cross. When the stage was turning round at an angle of 360(o), the Malta cross showed four times of extinction. In the vibrating direction of 45(o) angle of relative to upper and lower polarizing plate, gypsum test-board with optical path difference of 530nm was inserted, the first and the third quadrants of Malta cross appeared to be blue, and the second and the fourth quadrants appeared orange. When mica test-board with optical path difference of 147nm was inserted, the first and the third quadrants of Malta cross appeared yellow, and the second and the fourth quadrants appeared dark grey.CONCLUSION:The bile liquid crystals were distributed in bile in the form of global grains. Their polychroism and absorption were slight, but the edge and Becke-Line were very clear. Its refractive index was larger than that of the bile. These liquid crystals were uniaxial positive crystals. The interference colors were the first order grey-white. The double refractive index of the liquid crystals was n = 0.011-0.015.