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BACKGROUND AND AIMS: Identify novel metabolite associations with blood pressure (BP) salt-sensitivity and hypertension. METHODS AND RESULTS: The Genetic Epidemiology Network of Salt Sensitivity (GenSalt) Replication study includes 698 Chinese participants who underwent a 3-day baseline examination followed by a 7-day low-sodium feeding and 7-day high-sodium feeding. Latent mixture models identified three trajectories of blood pressure (BP) responses to the sodium interventions. We selected 50 most highly salt-sensitive and 50 most salt-resistant participants for untargeted metabolomics profiling. Multivariable adjusted mixed logistic regression models tested the associations of baseline metabolites with BP salt-sensitivity. Multivariable adjusted mixed linear regression models tested the associations of BP salt-sensitivity with metabolite changes during the sodium interventions. Identified metabolites were tested for associations with hypertension among 1,249 Bogalusa Heart Study (BHS) participants using multiple logistic regression. Fifteen salt-sensitivity metabolites were associated with hypertension in the BHS. Baseline values of serine, 2-methylbutyrylcarnitine and isoleucine directly associated with high salt-sensitivity. Among them, serine indirectly associated with hypertension while 2-methylbutyrylcarnitine and isoleucine directly associated with hypertension. Baseline salt-sensitivity status predicted changes in 14 metabolites when switching to low-sodium or high-sodium interventions. Among them, glutamate, 1-carboxyethylvaline, 2-methylbutyrylcarnitine, 3-methoxytyramine sulfate, glucose, alpha-ketoglutarate, hexanoylcarnitine, gamma-glutamylisoleucine, gamma-glutamylleucine, and gamma-glutamylphenylalanine directly associated with hypertension. Conversely, serine, histidine, threonate and 5-methyluridine indirectly associated with hypertension. Together, these metabolites explained an additional 7% of hypertension susceptibility when added to a model including traditional risk factors. CONCLUSIONS: Our findings contribute to the molecular characterization of BP response to sodium and provide novel biological insights into salt-sensitive hypertension.

BACKGROUND: To investigate the most appropriate dual antiplatelet therapy (DAPT) duration for patients with acute coronary syndrome (ACS) after drug-eluting stent (DES) implantation in the largest cardiovascular center of China. METHODS: We enrolled 5,187 consecutive patients with ACS who received DES from January to December 2013. Patients were divided into four groups based on DAPT duration: standard DAPT group (11–13 months, n=1,568) and prolonged DAPT groups (13–18 months [n=308], 18–24 months [n=2,125], and >24 months [n=1,186]). Baseline characteristics and 5-year clinical outcomes were recorded. RESULTS: Baseline characteristics were similar across the four groups. Among the four groups, those with prolonged DAPT (18–24 months) had the lowest incidence of major adverse cardiovascular and cerebrovascular events (MACCEs) (14.1% vs. 11.7% vs. 9.6% vs. 24.2%, P

Triple-negative breast cancer (TNBC) is a serious health issue for women worldwide and there is still no suitable treatment option. AA005, a structurally simplified mimic of natural Annonaceous acetogenins, presents outstanding properties with impressive cytotoxicity and cell-type selective actions. The present study was aimed at evaluating the potential of AA005 as a therapeutic agent for TNBC. AA005 potently inhibited the growth of TNBC cells at 50 nM level. Inspired by the finding of the phosphatase and tensin homologue (PTEN) tumor suppressor, the effect of AA005 on aerobic glycolysis was investigated in TNBC MDA-MB-468 cells. A short-term AA005 exposure markedly suppressed mitochondrial function in MDA-MB-468 cells, thus activating the aerobic glycolysis to lessen the risk of decreased ATP generation in mitochondria. Prolonging the incubation time of AA005 clearly weakened the aerobic glycolysis in the cells. This was in part attributed to the PI3K-AKT pathway inactivation and subsequent declined glucose uptake. As a consequence, the energy supply was completely cut from the two major energy-producing pathways. Further experiments showed that AA005 resulted in irreversible damage on cell activity including cell cycle and growth, inducing mitochondrial oxidative stress and ultimately leading to cell death. In addition, the in vivo therapeutic efficacy of AA005 was proved on 4T1 xenograft tumor mice model. Our data demonstrate that AA005 exhibited a great potential for future clinical applications in TNBC therapy.

Recently, a SU(4) $\pi$-flux Hubbard model on the honeycomb lattice has been proposed to study the spin-orbit excitations of $\alpha$-ZrCl$_3$ [Phys.~Rev.~Lett. 121.097201~(2017)]. Based on this model with a zigzag edge, we show the edge defects can induce edge flat bands that result in a SU(4) edge ferromagnetism. We develop an effective one-dimensional interaction Hamiltonian to study the corresponding SU(4) spin excitations. Remarkably, SU(4) spin excitations of the edge ferromagnet appear as a continuum covering the entire energy region rather than usual magnons. Through further entanglement entropy analysis, we suggest that the continuum consists of fractionalized spin excitations from the disappeared magnons, except for that from the particle-hole Stoner excitations. Moreover, in ribbon systems with finite widths, the disappeared magnons can be restored in the gap formed by the finite-size effect and the optical branch of the restored magnons are found to be topological nontrivial.

To observe the efficacy and prognosis of cladribine (2-CdA) combined with cytarabine (Ara-C) regimen in the treatment of relapsed refractory Langerhans cell histiocytosis (LCH) in children.Nine patients with relapsed refractory LCH treated with the 2-CdA combined with Ara-C regimen in the Department of Hematology and Oncology of Wuhan Children's Hospital from July 2014 to February 2020 were retrospectively analyzed, and the efficacy and disease status were evaluated according to the Histiocyte Society Evaluation and Treatment Guidelines (2009) and the Disease Activity Score (DAS), the drug toxicity were evaluated according to the World Health Organization（WHO） grading criteria for chemotherapy. All patients were followed up for survival status and disease-related sequelae.Before the treatment combining 2-CdA and Ara-C, 7 of 9 patients were evaluated as active disease worse (ADW), and 2 as active disease stable (ADS) with a median disease activity score of 8 (4-15). Of 9 patients, 6 cases achieved non active disease (NAD) and 3 achieved active disease better (ADB) with a median disease activity score of 0 (0 to 5) after 2-6 courses of therapy. All 9 patients experienced WHO grade IV hematologic toxicity and 3 patients had hepatobiliary adverse effects (WHO grade I～II) after treatment. The median follow-up time was 31(1 to 50) months with all 9 patients survived, 3 of the 9 patients experienced sequelae to the disease with 2 combined liver cirrhosis as well as cholestatic hepatitis and 1 with oral desmopressin acetate tablets for diabetes insipidus.2-CdA combined with Ara-C is an effective regimen for the treatment of recurrent refractory LCH in children, and the main adverse effect is hematologic toxicity, which is mostly tolerated in children. Early treatment with this regimen may be considered for patients with multisystem LCH with risky organ involvement who have failed first-line therapy and for patients with relapse.克拉屈滨联合阿糖胞苷治疗儿童复发难治性朗格汉斯细胞组织细胞增生症.观察克拉屈滨（Cladribine，2-CdA）联合阿糖胞苷（Ara-C）方案治疗儿童复发难治性朗格汉斯细胞组织细胞增生症（LCH）的疗效及预后.回顾性分析2014年7月至2020年2月于武汉儿童医院血液肿瘤科接受2-CdA联合Ara-C方案治疗的复发难治性LCH患儿9例，根据国际组织细胞协会LCH评估指南以及疾病活动度评分（DAS）对疗效以及疾病状态进行评估，根据世界卫生组织（WHO）化疗药物不良反应分级标准进行药物不良反应评估，随访患者生存状态以及疾病相关后遗症.在接受2-CdA联合Ara-C方案治疗前，9例患者中有7例评估为活动性疾病进展（ADW），2例评估为活动性疾病稳定（ADS），中位DAS为8分（4-15分）。经过（2-6）个该疗程治疗后，有6例患者达到无活动性疾病（NAD），3例达到活动性疾病好转（ADB），中位DAS为0分（0-5分）。9例患者在治疗后均出现血液系统不良反应（WHO IV级），3例患者出现肝胆系统不良反应（WHO I-II级）。中位随访时间31（1-50）个月，9例患者均存活，3例出现疾病相关后遗症，2例合并肝硬化以及胆汁淤积性肝炎、1例因中枢性尿崩症口服醋酸去氨加压素片.2-CdA联合Ara-C是治疗儿童复发难治性LCH的有效方案，主要药物不良反应为血液学毒性，儿童多可耐受。对于一线治疗失败的多系统LCH伴危险器官受累患者以及复发患者可考虑尽早使用2-CdA联合Ara-C方案治疗.

Obesity is a chronic disease that increases the risk of type II diabetes, heart diseases and nonalcoholic fatty liver disease. Unfortunately, to date, only a handful of drugs are approved for clinical use. This study aims at the discovery of anti-obesity agents based on naturally sourced oleanolic acid (OA) derivatives. 3T3-L1 preadipocytes were differentiated into mature adipocytes for in vitro assays, and a high-fat diet (HFD)-induced obesity mice model was established for in vivo studies. The screening of the OA derivatives was performed with 3T3-L1 cell, and resulted in a discovery of a novel compound HA-20 with a potent inhibitory activity on 3T3-L1 adipogenesis. In vitro data demonstrated that HA-20 markedly suppressed the adipogenesis in 3T3-L1 at the early stage without cytotoxicity. In vivo research using HFD mice revealed that HA-20 lowered the body weight, and possessed a lipid-lowering effect. Transcriptome analysis discovered that the mainly adipogenesis/lipogenesis genes regulated by HA-20 were Pparg, Cebpa, Fas, Acc, and Fabp4/aP2. Mechanism study revealed that HA-20 played its bioactive roles at least via downregulating PPARγ-FABP4/aP2 pathway in 3T3-L1, which was further confirmed in HFD-induced obesity mice. Our findings provided a new insight into fighting fat accumulation based on OA derivatives, and demonstrated that HA-20 may sever as a worthy leading compound for the further development of anti-obesity agents.

Excitons in solid state are bosons generated by electron-hole pairs as the Coulomb screening is sufficiently reduced. The exciton condensation can result in exotic physics such as super-fluidity and insulating state. In charge density wave (CDW) state, 1T-TiSe2 is one of the candidates that may host the exciton condensation. However, to envision its excitonic effect is still challenging, particularly at the two-dimensional limit, which is applicable to future devices. Here, we realize the epitaxial 1T-TiSe2 bilayer, the two-dimensional limit for its 2 × 2 × 2 CDW order, to explore the exciton-associated effect. By means of high-resolution scanning tunneling spectroscopy and quasiparticle interference, we discover an unexpected state residing below the conduction band and right within the CDW gap region. As corroborated by our theoretical analysis, this mysterious phenomenon is in good agreement with the electron-exciton coupling. Our study provides a material platform to explore exciton-based electronics and opto-electronics.

We employ the fluctuation-exchange approximation to study the relation of superconducting pairing symmetries and density-wave fluctuations based on the extended triangular Hubbard model upon electron doping and interactions, with an possible application to the layered metal dichalcogenide SnSe

To analyze the clinical effects of CCLG-AML-2015 protocol on newly diagnosed children with acute myeloid leukemia (AML).The clinical data of 60 newly diagnosed AML children in the Department of Hematology and Oncology, Wuhan Children's Hospital from August 2015 to September 2019 were summarized, the effect of chemotherapy using the CCLG-AML-2015 regimen (hereinafter referred to as the 2015 regimen) were retrospectively analyzed. 42 children with AML treated by the AML-2006 regimen (hereinafter referred to as the 2006 regimen) from February 2010 to July 2015 were used as control group.There were no statistical differences between the 2015 regimen group and the 2006 regimen group in sex, age at first diagnosis, and risk stratification (P0.05). The complete remission rate of bone marrow cytology after induction of 1 course of chemotherapy (84.7% vs 73.1%, P=0.155), and minimal residual disease detection (MRD) negative (42.3% vs 41.4%, P=0.928) in the 2015 regimen group were not statistically different than those in the 2006 regimen group. The bone marrow cytology CR (98.1% vs 80.6%, P=0.004) and MRD negative (83.3% vs 52.8%, P=0.002) in the 2015 regimen group after 2 courses of induction were higher than those in the 2006 regimen group. The 5-year overall survival (OS) rate in the 2015 regimen group (62.3%±6.4% vs 20.6%±6.4%, P=0.001), the 5-year disease-free survival (EFS) rate (61.0%±6.4% vs 21.0% ±6.4% , P=0.001) were better than those in the 2006 regimen group. The 5-year OS and EFS of high-risk transplant patients in the 2015 regimen group were significantly better than those of high-risk non-transplant patients (OS: 86.6%±9.0% vs 26.7%±11.4%, P=0.000; EFS: 86.6%±9% vs 26.7%±11.4%, P=0.000).The 2015 regimen can increase the CR rate after 2 courses of induction compared with the 2006 regimen. High-risk children receiving hematopoietic stem cell transplantation can significantly improve the prognosis.CCLG-AML-2015方案治疗儿童急性髓系白血病的临床分析.分析CCLG-AML-2015方案治疗初诊儿童急性髓系白血病（AML）的临床效果.总结2015年8月至2019年9月武汉儿童医院血液肿瘤科60例初诊AML患儿的临床资料，回顾性分析使用CCLG-AML-2015方案（以下简称2015方案）化疗的效果，对照组为本中心2010年2月-2015年7月使用AML-2006方案（以下简称2006方案）治疗的42例AML患儿.2015方案组与2006方案组患者在性别、初诊年龄、危险度分层均无统计学差异（P＞0.05）。2015方案组诱导1个疗程化疗后骨髓细胞学完全缓解率（CR，84.7% vs 73.1%，P=0.155）、微小残留病灶检测（MRD）阴性（42.3% vs 41.4%，P=0.928）与 2006方案组比较无统计学差异，诱导2个疗程后骨髓细胞学CR（98.1% vs 80.6%，P=0.004）及MRD阴性（83.3% vs 52.8%，P=0.002）高于AML-2006方案组。2015方案组5年总生存（OS）率（62.3%±6.4% vs 20.6%±6.4%，P=0.001），5 年无病生存（EFS）率（61.0%±6.4% vs 21.0%±6.4%，P=0.001）均优于2006方案组。2015方案组高危患儿接受移植5年OS、EFS均明显优于高危非移植患儿（86.6%±9.0% vs 26.7%±11.4%，P=0.000；86.6%±9% vs 26.7%±11.4%，P=0.000）.2015方案较2006方案可提高诱导2个疗程后CR率，高危患儿接受造血干细胞移植可显著改善预后.

Fluid catalytic cracking (FCC) slurry is a by-product of petrochemical industry rich in aromatics. In this paper, the effect of FCC slurry on the physical properties of SBS modified bitumen was investigated by softening point, penetration, ductility and low temperature flexibility test. The influence of FCC slurry on the compatibility of SBS modified bitumen was evaluated by fluorescence microscope and segregation test. The results show that FCC slurry improves the softening point, penetration, ductility and low temperature flexibility of SBS modified bitumen. Fluorescence microscope showed that FCC slurry promotes the dispersion of SBS in bitumen, which is conducive to the formation of network crosslinking structure of SBS modified bitumen. The segregation test indicated that the compatibility of SBS with bitumen was improved with the content of FCC slurry increasing.

Background Solute Carrier Family 6 Member 1 (SLC6A1) has been identified as a cancer-promoting gene in various human cancers, such as clear cell renal cell carcinoma and ovarian cancer. However, its roles in prostate cancer (PCa) has not been fully elucidated. The aim of this study was to investigate the expression and clinical significance of SLC6A1 in PCa tissues and its effect on drug resistance to docetaxel in PCa. Methods Expression patterns of SLC6A1 protein in PCa tissues were examined by immunohistochemistry based on Tissue microarray. Associations of SLC6A1 protein expression with various clinicopathological features and patients’ prognosis of PCa were also statistically evaluated based on TCGA data. Roles of SLC6A1 deregulation in prostate carcinogenesis and drug resistance was further determined in vitro and in vivo experiments. Results Based on TCGA Dataset, SLC6A1 expression was markedly higher in patients with high Gleason score, advanced clinical stage and positive biochemical recurrence than those with control features (all P Conclusions Our data suggest that SLC6A1 overexpression may be associated with aggressive progression and short biochemical recurrence-free survival of PCa, and may be related to the resistance to docetaxel therapy.

The isoindolinone and biaryl scaffolds are prevalent in natural products and drug molecules, which have showed broad and interesting biological activities. The efficient construction of such hybridized molecules and biological evaluation are of great interest to medicinal chemistry community. In this communication, we report an efficient Bronsted acid-promoted C(sp3)-H functionalization approach that enables the rapid construction of biologically important isoindolinone/[1,2,4]triazolo[1,5-a]pyrimidine hybrids from 5-methyl-7-(2,4,6-trimethoxyphenyl)-[1,2,4]triazolo[1,5-a]pyrimidine, 2-formylbenzoic acid and various anilines. The title compounds were generated in high to excellent yields (up to 96%) regardless of the electronic nature and steric effects of the substituents. In this reaction, an isoindolinone scaffold, one C C single bond, and two C N bonds were formed simultaneously with high atom economy. In this work, we have envisioned that the methyl group linked to the electron-deficient N-heterocycles could be used as a new synthetic handle for late-state diversification and may have broad applications in the field of organic and medicinal chemistry. Besides, the title compounds have exhibited promising activity against the SKP2-CKS1 interaction.

Five new triterpenoid saponin glycosides, trichocucumerisides A–E (1–5), together with eleven known compounds (6–16) were isolated from Trichosanthes cucumerina fruit fibers. The structures of the new compounds were elucidated by detailed analysis of NMR and mass spectroscopic data as well as chemical reactions. The anti-inflammatory study against nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells shows that compounds 7 and 9 exhibited stronger NO inhibitory activity, with IC50 values of 3.0 and 2.7 μM, respectively, with comparison to positive references Celecoxib and aminoguanidine (IC50 values 75.7 and 75.0 μM, respectively). Compounds 7 and 9 also possessed a greater selectivity index (SI) of approximately 3–4-fold activity than that of the positive references.

The rapid improvement of technologies such as artificial intelligence in recent years has resulted in the development of smart technologies (ST) that can influence learning performance in different fields. The purpose of study is to explore the link between smart technology and learning performance. Using the S-O-R model as a framework, the researchers argue that smart technology (Stimuli) will increase corporate trust, self-efficacy, and well-being (Organism), resulting in improved learning performance (Response). The current model regards corporate trust and self-efficacy as relationship factors and investigates their direct influence on employee well-being and learning performance and the mediating role played by these variables. Additionally, the function of employee well-being in moderating the relationship between corporate trust, self-efficacy, and employee learning performance is also explored. The respondents (n = 516) in the present study are made up of employees from 10 logistics companies located in China. The data analysis is conducted using the AMOS software. The results show that that smart technologies can affect learning performance through corporate trust, self-efficacy, and employee well-being. The implementation of smart technology initiatives by corporations may provide positive workplace outcomes for employees (increased well-being), corporations (more engagement in workplace learning performance), and the relationship between employees and the companies that employ them (corporate trust and self-efficacy).

We introduce the spin entanglement entropy (EE) to characterize spin excitations. The scalings of EEs are elaborated as $\ln N$, $\ln N+D_k$ and $2\ln N+D$ for the single-magnon states, two-magnon bound states and two-magnon continuum, respectively, based on the Bethe ansatz solutions of the ferromagnetic spin chain with $N$ sites. The $\ln N$ divergence for the bound states reveals the two magnons emerge as a new quasiparticle. More importantly, the nonzero intercepts ($D_k,D$) embody the many-body effects of two-magnon states. In particular, an exact relation between the intercept of EEs and an observable quantity, the two-magnon distance, is established for the bound states. In such a case, we quantify the two-magnon confinement in a spin system by the increasing entanglement with the distance as a particle physics analogue. Moreover, the EEs can also be used to study the evolution of the excitations. When the bound states are immersed in the continuum in the alternating chain, they undergo an oscillating confinement-deconfinement crossover, shown by the oscillations of the EEs and two-magnon distance with the chain length., Comment: 5 pages, 3 figures

$\alpha$-RuCl$_3$ has been studied extensively because of its proximity to the Kitaev quantum-spin-liquid (QSL) phase and the possibility of approaching it by tuning the competing interactions. Here we present the first polarized inelastic neutron scattering study on $\alpha$-RuCl$_3$ single crystals to explore the scattering continuum around the $\Gamma$ point at the Brillouin zone center, which was hypothesized to be resulting from the Kitaev QSL state but without concrete evidence. With polarization analyses, we find that while the spin-wave excitations around the M point vanish above the transition temperature $T_{\rm N}$, the pure magnetic continuous excitations around the $\Gamma$ point are robust against temperature. Furthermore, by calculating the dynamical spin-spin correlation function using the cluster perturbation theory, we derive magnetic dispersion spectra based on the $K$-$\Gamma$ model, which involves with a ferromagnetic Kitaev interaction of -7.2 meV and an off-diagonal interaction of 5.6 meV. We find this model can reproduce not only the spin-wave excitation spectra around the M point, but also the non-spin-wave continuous magnetic excitations around the $\Gamma$ point. These results provide evidence for the existence of fractional excitations around the $\Gamma$ point originating from the Kitaev QSL state, and further support the validity of the $K$-$\Gamma$ model as the effective minimal spin model to describe $\alpha$-RuCl$_3$., Comment: Published in Chinese Physics Letters as Express Letter, 8 pages, 4 figures

Disorder is important in the study of quantum spin liquids, but its role on the spin dynamics remains elusive. Here, we explore the disorder effect by investigating the magnetic-field dependence of the low-energy magnetic excitations in a triangular-lattice frustrated magnet YbZnGaO$_4$ with inelastic neutron scattering. With an intermediate field of 2.5 T applied along the $c$-axis, the broad continuum at zero field becomes more smeared both in energy and momentum. With a field up to 10 T, which fully polarizes the magnetic moments, we observe clear spin-wave excitations with a gap of $\sim$1.4 meV comparable to the bandwidth. However, the spectra are significantly broadened. The excitation spectra both at zero and high fields can be reproduced by performing classical Monte Carlo simulations which take into account the disorder effect arising from the random site mixing of nonmagnetic Zn$^{2+}$ and Ga$^{3+}$ ions. These results elucidate the critical role of disorder in broadening the magnetic excitation spectra and mimicking the spin-liquid features in frustrated quantum magnets., Comment: Published in PRB, 10 pages, 3 figures

To investigate the clinical characteristics of infection in children with acute myeloid leukemia (AML) after high intensive chemotherapy， so as to provide reference for prevention and control of infection.56 children diagnosed as acute myeloid leukemia in our hospital from January 2016 to August 2019 were enrolled and retrospectively analyzed, the infection rate, pathogens of disease and common location of infection during the induction and consolidation period were analyzed.The total infection rate of the patients was 93.4%－96.4%, the average of serious infection rate was 16.0%(11.3%－19.6%), and the infection related mortality was 10.7%. Fever of unknown cause was the main reason of infection, while blood flow infections were the most common in severe infection, which were mainly caused by Gramnegative bacteria. The rate of fungal infection was 35.7% during chemotherapy.Children with AML shows a high incidence of infection in each stage of chemotherapy. The serious illness caused by blood flow infection and take antifungal drugs to reduce the occurrence of fungal infection in AML patients should be paid attention.高强度化疗时代下儿童急性髓系白血病感染的临床研究.研究儿童急性髓系白血病（AML）患儿在高强度化疗后感染的临床特点并探索防控措施.选取2016年1月－2019年8月本院初诊AML患儿56例，分析总结其在诱导、巩固期感染的发生率、病原菌及感染部位.AML患儿各阶段感染率在93.4%－96.4%之间，严重感染率平均值为16.0%（11.3%－19.6%），发生感染相关的死亡为10.7%。不明原因性发热是感染主要原因，而血流感染则常见于严重感染，以革兰氏阴性菌感染为主。化疗期间真菌感染率为35.7.儿童AML各化疗阶段均出现高感染发生率，重视血流感染导致的严重感染，预防性使用抗真菌药物以减少AML合并的真菌感染.

BACKGROUND Association between tea consumption and incident hypertension remains uncertain. This study conducted to examine the health effects of tea consumption on blood pressure progression and hypertension incidence. METHODS A population-based cohort of 38,913 Chinese participants without hypertension at baseline were included in the current study. Information on tea consumption was collected through standardized questionnaires. Associations of tea consumption with blood pressure progression and incident hypertension were analyzed using logistic regression models and Cox proportional hazards regression models, respectively. RESULTS During a median follow-up of 5.9 years, 17,657 individuals had experienced progression to a higher blood pressure stage and 5,935 individuals had developed hypertension. In multivariate analyses, habitual tea drinkers (≥ 3 times/week for at least six months) had a 17% lower risk for blood pressure progression [odds ratio (OR) = 0.83, 95% CI: 0.79–0.88] and a 14% decreased risk for incident hypertension [hazard ratio (HR) = 0.86, 95% CI: 0.80–0.91] compared with non-habitual tea drinkers. Individuals in different baseline blood pressure groups could obtain similar benefit from habitual tea drinking. In terms of tea consumption amount, an inverse, linear dose-response relation between monthly consumption of tea leaves and risk of blood pressure progression was observed, while the risk of incident hypertension did not reduce further after consuming around 100 g of tea leaves per month. CONCLUSIONS Our study demonstrated that habitual tea consumption could provide preventive effect against blood pressure progression and hypertension incidence.

Metabolic-associated fatty liver disease (MAFLD) is caused by hepatocyte steatosis and is associated with obesity, type II diabetes, and heart disease. There are currently no effective drugs to treat MAFLD. This study explored the effect of HA-20, an oleanolic acid derivative, on hepatocyte steatosis in MAFLD. HepG2, L02, and AML12 cells were developed using oleic acid for in vitro MAFLD cell assays, and a high-fat diet + high-fructose diet-induced (HFHF) MAFLD mouse model was established for in vivo studies. The results demonstrated that HA-20 prevented hepatocyte steatosis in cell assays and caused 26.3, 57.7 and 70.0% inhibition of triglyceride (TG) levels in the 5.0, 10.0 and 20.0 μM HA-20 groups, respectively. The EC

As a special order of electronic correlation induced by spatial modulation, the charge density wave (CDW) phenomena in condensed matters attract enormous research interests. Here, using scanning–tunneling microscopy in various temperatures, we discover a hidden incommensurate stripe-like CDW order besides the ( 7 × 3 ) CDW phase at low-temperature of 4 K in the epitaxial monolayer 1T-VSe2 film. Combining the variable-temperature angle-resolved photoemission spectroscopic (ARPES) measurements, we discover a two-step transition of an anisotropic CDW gap structure that consists of two parts Δ 1 and Δ 2. The gap part Δ 1 that closes around ∼ 150 K is accompanied with the vanish of the ( 7 × 3 ) CDW phase. While another momentum-dependent gap part Δ 2 can survive up to ∼ 340 K, and is suggested to the result of the incommensurate CDW phase. This two-step transition with anisotropic gap opening and the resulted evolution in ARPES spectra are corroborated by our theoretical calculation based on a phenomenological form for the self-energy containing a two-gap structure Δ 1 + Δ 2, which suggests different forming mechanisms between the ( 7 × 3 ) and the incommensurate CDW phases. Our findings provide significant information and deep understandings on the CDW phases in monolayer 1T-VSe2 film as a two-dimensional (2D) material.

To explore the relationship between plasma sST2/Reg3α levels and acute graft-versus-host disease (aGVHD) in children after allogeneic hematopoietic stem cell transplantation (allo-HSCT).The clinical data of 29 pediatric patients received allo-HSCT treatment in Department of Hematology and Oncology of Wuhan Children's Hospital from January 2019 to January 2020 were collected. Peripheral blood samples were collected at 14 and 28 day after allo-HSCT. The plasma concentrations of sST2 and Reg3α were detected by Luminex assay.Among 29 patients there were 15 males and 14 females with a median age of 53 (29-117) months. After allo-HSCT, 18 patients developed grade 0-I aGVHD; while 11 patients developed grade II-IV aGVHD. These included skin aGVHD in 6 cases, gastrointestinal aGVHD (GI-aGVHD) in 3 cases and gastrointestinal/skin aGVHD in 5 cases. Plasma sST2 level in II-IV aGVHD group showed significantly higher than that in 0-I aGVHD group at 28 days after allo-HSCT [101.81 (73.94-150.77) ng/ml vs 48.97 (28.82-56.69) ng/ml, P=0.021]. Also, the plasma sST2 level was significantly higher in GI-aGVHD group than that in no-aGVHD group at 28 days after allo-HSCT [118.74 (87.00-243.36) ng/ml vs 48.97 (23.55-61.40) ng/ml, P=0.004]. Plasma sST2 level ≥65.34 ng/ml at 28 days after allo-HSCT showed a sensitivity of 85.7% and a specificity of 87.5% in predicting II-IV aGVHD. And the patients with a plasma sST2 level ≥65.34 ng/ml showed a significantly higher incidence of II-IV aGVHD than those with plasma sST2 level of65.34 ng/ml after allo-HSCT (P=0.021). There was no significant difference in plasma Reg3α level between the patients with II-IV aGVHD and the non-aGVHD ones.The increasing plasma sST2 level after allo-HSCT in children indicates the development of II-IV aGVHD, so sST2 is promising as a biomarker for predicting II-IV aGVHD.儿童异基因造血干细胞移植后血浆sST2/Reg3α蛋白水平与急性移植物抗宿主病的相关性.探讨儿童异基因造血干细胞移植（allo-HSCT）后血浆sST2/Reg3α蛋白动态变化与急性移植物抗宿主病（aGVHD）的相关性.收集2019年1月至2020年1月在武汉儿童医院血液肿瘤科接受allo-HSCT的患儿29例的临床资料。采集患者移植后14 d和28 d外周血标本，使用Luminex方法检测血浆sST2/Reg3α蛋白浓度.29例患儿中男性15例，女性14例，中位年龄53（29-117）个月。接受移植后0-I度aGVHD 18例，II-IV度aGVHD 11例；皮肤aGVHD 6例，肠道aGVHD（GI-aGVHD）3例，肠道及皮肤aGVHD 5例。II-IV度aGVHD组与0-I度aGVHD组移植后28 d血浆sST2蛋白水平分别为101.81（73.94-150.77）ng/ml与48.97（28.82-56.69）ng/ml，差异具有统计学意义（P=0.021）。GI-aGVHD组与无aGVHD组患者移植后28 d血浆sST2蛋白水平分别为118.74（87.00-243.36）ng/ml和48.97 （23.55-61.40） ng/ml，差异具有显著性（P=0.004）。当移植后28 d血浆sST2蛋白浓度≥ 65.34 ng/ml时预测II-IV度aGVHD的敏感度为85.7%，特异性为87.5%；且移植后血浆sST2蛋白高水平组（≥65.34 g/ml）患者II-IV度aGVHD发生率显著高于低水平组（65.34 ng/ml）（P=0.021）。Reg3α蛋白在II-IV度aGVHD组与无aGVHD组的差异无统计学意义.儿童allo-HSCT后血浆sST2蛋白水平升高提示II-IV度aGVHD的发生。高水平sST2蛋白有望作为预测II-IV度aGVHD发生的生物学诊断标志.

To summarize the long-term efficacy of cyclosporine (CsA) in the treatment of non-severe aplastic anemia (NSAA) in children, and explore the early significant indicators.Data of 36 NSAA children in Department of Hematological Oncology, Wuhan Children's Hospital, Tongji Medical College of Huazhong University of Science and Technology from January 2013 to December 2017 were analyzed retrospectively. All the children received oral CsA immunosuppressive therapy, and CsA trough concentration was checked to maintain at the rage of 200-250 μg/L after 2 weeks. The evaluation time points were at 3, 6, 12, 18 and 24 months, and assessment items were peripheral white blood cell differential count and reticulocyte's percentage and count.The 36 NSAA cases were composed of 16 males and 20 females, whose median age was 5.46 (2.92-7.99) years old, and median follow-up time was 28.00 (10.00-38.25) months. After taking oral CsA for 24 months, the number of cumulative effective cases was 21. There were 4 cases of complete remission (CR), 17 cases of partial remission (PR), and 15 cases of non-remission (NR). The total effective rate was 58.33%, and median effect-acting time of CsA was 3.0 (0.5-10.0) months. Compared with ineffective group, neutrophil (NEU) and red blood cell (RBC) of effective group (CR+PR) began to increase significantly at the 3rd month, and hemoglobin (Hb), platelet (PLT) and white blood cell (WBC) increase significantly at the 6th month after oral CsA administration (P0.05). Except for 2 cases who received component transfusion within 3-12 months after taking oral CsA for 3 months in effective group, the others did not need.The overall effective rate of oral CsA in children with NSAA was 58.33%. Stopping blood transfusion after the 3 months of treatment may be considered as a turning point for disease outcomes, and levels of NEU, RBC at the 3rd month and Hb, PLT, WBC at the 6th month as indicators for predicting disease prognosis.环孢素A治疗儿童非重型再生障碍性贫血的转归效果及预后因素分析.总结环孢素（CsA）治疗儿童非重型再生障碍性贫血（NSAA）的长期效果，并探讨与CsA治疗NSAA起效和预后相关的早期指标.回顾性分析2013年1月-2017年12月在华中科技大学同济医学院附属武汉儿童医院住院的36例NSAA患儿的病历资料，所有患儿在确诊后均接受CsA口服治疗，2周后检查CsA谷浓度以维持在200-250 μg/L范围，评估时间点为口服CsA后3、6、12、18和24个月，评估项目为外周血细胞分类计数及网织红细胞百分比与计数.36例患儿中男性16例，女性20例，中位年龄5.46（2.92-7.99）岁，中位随访时间28.00（10.00-38.25）个月。口服CsA后24个月临床治疗有效的累计病例数为21例。完全缓解4例，部分缓解17例，无效15例，总有效率58.33%，药物起效的中位时间为3.0（0.5-10.0）个月。与无效组比较，有效组（完全缓解和部分缓解）患儿表现为口服CsA后第3个月中性粒细胞和红细胞显著升高，第6个月血红蛋白、血小板、白细胞显著升高（P0.05）；口服CsA 3个月后有效组除2例患儿在3-12个月内进行成分输血外，其余19例患儿均不再需要成分输血支持治疗.NSAA患儿口服CsA总体有效率为58.33%。治疗3个月后是否停止输注血液制品可作为疾病转归的转折点，治疗3个月时的中性粒细胞、红细胞和6个月时的血红蛋白、血小板、白细胞水平可作为评估疾病转归的指标.

Topological magnons are bosonic analogues of topological fermions in electronic systems. They have been studied extensively by theory but rarely realized by experiment. Here, by performing inelastic neutron scattering measurements on single crystals of a two-dimensional ferromagnet CrBr$_3$, which was classified as Dirac magnon semimetal featured by the linear bands crossing at the Dirac points, we fully map out the magnetic excitation spectra, and reveal that there is an apparent gap of $\sim$3.5~meV between the acoustic and optical branches of the magnons at the K point. By collaborative efforts between experiment and theoretical calculations using a five-orbital Hubbard model obtained from first-principles calculations to derive the exchange parameters, we find that a Hamiltonian with Heisenberg exchange interactions, next-nearest-neighbor Dzyaloshinskii-Moriya (DM) interaction, and single-ion anisotropy is more appropriate to describe the system. Calculations using the model show that the lower and upper magnon bands separated by the gap exhibit Chern numbers of $\pm1$. These results indicate that CrBr$_3$ is a topological magnon insulator, where the nontrivial gap is a result of the DM interaction., Version as published in PRB Letter, main text 7 pages, supplementary materials 6 pages

In vivo monitoring of cargo protein delivery is critical for understanding the pharmacological efficacies and mechanisms during cancer therapy, but it still remains a formidable challenge because of the difficulty in observing nonfluorescent proteins at high resolution and sensitivity. Here we report an outer-frame-degradable nanovehicle featuring near-infrared (NIR) dual luminescence for real-time tracking of protein delivery in vivo. Upconversion nanoparticles (UCNPs) and fluorophore-doped degradable macroporous silica (DS) with spectral overlap were coupled to form a core-shell nanostructure as a therapeutic protein nanocarrier, which was eventually enveloped with a hyaluronic acid (HA) shell to prevent protein leakage and for recognizing tumor sites. The DS layer served as both a container to accommodate the therapeutic proteins and a filter to attenuate upconversion luminescence (UCL) of the inner UCNPs. After the nanovehicles selectively accumulated at tumor sites and entered cancer cells, intracellular hyaluronidase (HAase) digested the outermost HA protective shell and initiated the outer frame degradation-induced protein release and UCL restoration of UCNPs in the intracellular environment. Significantly, the biodistribution of the nanovehicles can be traced at the 710 nm NIR fluorescence channel of DS, whereas the protein release can be monitored at the 660 nm NIR fluorescence channel of UCNPs. Real-time tracking of protein delivery and release was achieved in vitro and in vivo by NIR fluorescence imaging. Moreover, in vitro and in vivo studies manifest that the protein cytochrome c-loaded nanovehicles exhibited excellent cancer therapeutic efficacy. This nanoplatform assembled by the outer-frame-degradable nanovehicles featuring NIR dual luminescence not only advances our understanding of where, when, and how therapeutic proteins take effect in vivo but also provides a universal route for visualizing the translocation of other bioactive macromolecules in cancer treatment and intervention.