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The detection of l-aspartic acid and l-glutamic acid was tackled using fluorescent microparticles constructed by the combination of aggregation-induced emission and self-assembly-induced Förster resonance energy transfer.

Background. Naoluo Xintong decoction (NLXTD) is a traditional Chinese medicine (TCM) formula which has been used to improve neuronal functional recovery after cerebral ischemic stroke. However, the molecular mechanism underlying NLXTD’s amelioration of ischemic stroke remains unclear. The present study was designed to explore the effect and mechanism of NLXTD on brain angiogenesis in a rat model with cerebral ischemia-reperfusion (I/R) injury targeting the hypoxia-inducible factor-1α (HIF-1α)/vascular endothelial growth factor (VEGF) pathway. Materials and Methods. Cerebral I/R model was established by the classical middle cerebral artery occlusion (MCAO) method. Sprague-Dawley (SD) male rats (n = 80) were randomly divided into the sham-operation group, the model group, the HIF-1α inhibitor 2-methoxyestradiol (2ME2) group, the 2ME2 with NLXTD group, and the NLXTD group. Neurological deficit test, TTC staining, H&E staining, TUNEL staining, immunohistochemistry (IH), immunofluorescence (IF), western blot, and quantitative RT-PCR were performed to evaluate the effect of NLXTD after MCAO. Results. Administration of NLXTD significantly decreased neuron deficiency scores, reduced brain infarct volume, and lowered damaged and apoptotic cells after brain I/R injury in rats. Meanwhile, NLXTD had a protective effect on angiogenesis by increasing the MVD and the expressions of BrdU and CD34, which enhanced the number of endothelial cells in the ischemic penumbra brain. NLXTD treatment significantly raised the protein and mRNA levels of HIF-1α, VEGF, VEGFR2, and Notch1 compared with the model treatment. In contrast, a specific HIF-1α inhibitor, 2ME2, inhibited the improvement of neurological function and angiogenesis in NLXTD-induced rats with cerebral I/R injury, suggesting that NLXTD played a positive role in ischemic brain injury by activating the HIF-1α/VEGF signaling pathway. Conclusions. NLXTD exerts neuroprotection targeting angiogenesis by upregulating the HIF-1α/VEGF signaling pathway on cerebral I/R injury rats.

We developed fluorescence sensor for detecting HCHO. Such molecular sensors have great potential to be utilized. We hope that this simple fluorescent probe can arouse widespread attention in the fields of molecular sensing and food chemistry.

The early detection of volatile organic compounds (VOCs) is crucial for sustainable development. Improving the binding properties of crystalline materials for special VOC guests is a tough challeng...

The similarities and differences between "Novel Coronavirus Infected pneumonia in Hunan province (trial second edition)" and "Novel Coronavirus Infected pneumonia (Trial seventh edition)" released by the National Health Commission and their ideas were interpreted based on “suiting measures to three factors”. ①In Hunan program, the third version of the trial adjustment more suitable for local characteristics of the disease; ②The prevention program population classification is clear, support, eliminate pathogenic factors of the primary and secondary distinct; ③ The cause of disease is prominent, damp and toxin are mixed with dryness, and the treatment is mainly to moisten the lung and clear away heat, and the prescription is mostly Xinliang light agent.

A supramolecular sensor (APRA–G) was successfully constructed via host–guest interactions between a rhodamine hydrazone functionalized pillar[5]arene (APRA) and a bis-pyridinium derivative (G), which showed highly selective and sensitive detection of toxic Hg2+ through a dual-channel (colorimetric and fluorescent) model. The detection limits of fluorescence and UV-vis spectroscopy towards Hg2+ were 1.69 × 10−8 M and 4.07 × 10−7 M, respectively, which were calculated using the 3σ method. Interestingly, APRA–G could act as an excellent material for Hg2+ separation. Based on ICP (including inductively coupled plasma) data, the separation percentage of the supramolecular sensor (APRA–G) for mercury ions was up to 99.68%.

Supramolecular gels have been widely reported on account of their unique superiority and application prospects. In this work, we constructed a novel supramolecular gel (HD-G) by using hydroxy-naphthaldehyde decorated with naphthalimide in DMSO solution, which exhibited excellent selectivity and ultrasensitive sensing properties toward CN− (the lowest detection limit is 1.82 × 10−10 M). The sensing mechanism of this supramolecular gel takes advantage of π–π stacking interactions and anion–π interactions, which is different from the other familiar methods.

Naoluo Xintong decoction (NLXTD) is a traditional Chinese medicine (TCM) formula which has been used to improve neuronal functional recovery after cerebral ischemic stroke. However, the molecular mechanism underlying NLXTD's amelioration of ischemic stroke remains unclear. The present study was designed to explore the effect and mechanism of NLXTD on brain angiogenesis in a rat model with cerebral ischemia-reperfusion (I/R) injury targeting the hypoxia-inducible factor-1Cerebral I/R model was established by the classical middle cerebral artery occlusion (MCAO) method. Sprague-Dawley (SD) male rats (Administration of NLXTD significantly decreased neuron deficiency scores, reduced brain infarct volume, and lowered damaged and apoptotic cells after brain I/R injury in rats. Meanwhile, NLXTD had a protective effect on angiogenesis by increasing the MVD and the expressions of BrdU and CD34, which enhanced the number of endothelial cells in the ischemic penumbra brain. NLXTD treatment significantly raised the protein and mRNA levels of HIF-1NLXTD exerts neuroprotection targeting angiogenesis by upregulating the HIF-1

A novel fluorescent chemosensor based on trimesoyl chloride-rhodamine (TR) was successfully synthesized. Rising chromogenic and fluorogenic spectral enhancements could be observed in trimesoyl chloride-rhodamine (TR) probes when Hg

Supramolecular gels have been widely reported on account of their unique superiority and application prospects. In this work, we constructed a novel supramolecular gel (HD-G) by using hydroxy-naphthaldehyde decorated with naphthalimide in DMSO solution, which exhibited excellent selectivity and ultrasensitive sensing properties toward CN

Stimuli-response supramolecular fluorescent materials have received significant attention in recent years since they endow specific and unique properties to materials. Herein, a bi-acylhydrazone supramolecular gelator HT was successfully prepared by simple Schiff base reaction. Interestingly, the gelator HT could self-assemble into supramolecular gel HT-G in DMSO-H2O binary solutions via π-π stacking interactions. Simultaneously, gel HT-G showed specially selective and ultrasensitive sensing property for CN−. The limit of lowest detection of the gel for CN− is 0.50 nM. In addition, the thin films based on the HT-G and HT-G + CN− were also prepared, which could not only act as supramolecular fluorescent material but a convenient and efficient tool for detection CN−.

In order to fabricate green composites without the emission of toxic volatile compounds, wood fiber (WF) biocomposites reinforced with polylactic acid (PLA) were prepared utilizing the traditional hot-press manufacturing process. The effect of adding ammonium lignosulfonate (AL) on the mechanical and dimensional properties of the biocomposites was investigated. When the mass ratio of WF to PLA is 7:3 and the AL mass percentage is in the range from 1 to 20 wt%, the modulus of rupture of the WF/PLA/AL composites ranged from 21.95 to 36.96 MPa, the modulus of elasticity ranged from 3078.6 to 4157.8 MPa and the internal bonding strength ranged from 0.69 to 1.71 MPa, which showed a 4.0–75.1% increase in modulus of rupture, a 3.5–39.8% increase in modulus of elasticity and a 21.5–201.2% increase in internal bonding strength compared to the corresponding WF/PLA composites due to the reinforcing effect of AL. However, AL negatively affected the dimensional properties of the biocomposites. The thickness swell of the WF/PLA/AL composites ranged from 18.7 to 43.5% and the water absorption ranged from 47.1 to 78.9%. The thickness swell, water absorption and contact angle of the WF/PLA/AL composites increased with the AL mass percentage due to its high hydrophilicity, resulting in a 1.1–132.6% and a 3.4–67.4% increase in thickness swell and water absorption, respectively. AL had a positive effect on the crystallinity of the biocomposites due to its good surface activity as long as the AL mass percentage was below 20 wt%. The fractured surface of the WF/PL/AL composites clearly showed that a strong bond (mechanical interlocking and/or chemical bonding) exists at the interface resulting in an effective stress transfer.

Background We investigated the activity of loureirin B against liver fibrosis and the underlying molecular mechanisms. Methods Hepatic stellate cells (HSCs) from Sprague-Dawley rats were treated with different concentrations of loureirin B. We used the MTT assay to determine HSC proliferation, flow cytometry to analyze apoptosis, and western blot to determine the expressions of Bax, Bcl-2, Wnt1 and β-catenin. Real-time PCR was used to determine the expressions of Wnt1 and miR-148-3p. Results The MTT assay showed that loureirin B treatment significantly inhibited the proliferation of HSCs in time- and dose-dependent manners. Loureirin B significantly promoted the apoptosis of HSCs, increased the expression of Bax and decreased the Bcl-2 level. Western blot analysis showed that the expressions of Wnt1 and β-catenin were obviously lower in the loureirin B treatment group than in the control group. We also found that loureirin B could decrease the Wnt1 mRNA level and increase miR-148-3p expression. Knockdown of miR-148-3p using inhibitor could reverse the effects of loureirin B on the proliferation and apoptosis of HSCs and the expressions of Bax, Bcl-2, Wnt1 and β-catenin. Conclusion Our results suggest that loureirin B inhibited the proliferation and promoted the apoptosis of HSCs, and suppressed the Wnt/β-catenin signaling pathway via regulation of miR-148-3p.

This paper study the object of the speed accessory and give the forming numerical simulation of its bending process based on software DYNAFORM. The simulation analysis the effects to its bending springback produced by four factors such as thickness of plate, die fillet, texture of material and the friction coefficient, and research on the factors affect the springback through the orthogonal experiment method, determine the impact factor of the four factors to the bending springback process and find the optimum technological parameters combination.

To pack a certain quality ratio ammonium lignosulfonate / urea as a filler into the natural wood fiber, Using flat pressure process fiberboard materials, Optimize the hot pressing technique that the filler is 20% (The quality ratio of lignosulfonate/ urea is 5: 3), slab moisture content is 13%, hot-pressing temperature is 190°C; hot-pressing time is 7 minutes, hot-pressing pressure is 2MPa and 1% paraffin emulsion. Verifying test results show that mechanical performance is stable and reliable process. Analyze the reactivity of fiberboard packing, fiber gathered, the microstructure of interface properties with the application of FTIR, XRD and ESEM and other modern instrument analysis technology.

In this paper, the production technology of laminated veneer lumber was optimized by using the uniform design method. The effects of production technology, such as the press duration, the press temperature, the press pressure and the adhesive spread, were found statistically significant on some mechanical properties of LVL. So in this paper, the thickness of LVL board was a fixed value, but the press duration, the press temperature and the adhesive spread were variable. The modulus of elasticity (MOE) and static bending strength (MOR) as predicted by the ANN approach and the regression functions were compared with the experimental values. It was shown that the proposed neural network model was able to predict valuable mechanical properties, such as the modulus of elasticity and static bending strength, that would facilitate the development of optimum design of properties for manufacturing high quality materials.

To compare the effects of three traditional Chinese medicinal compounds on energy metabolism related enzymes in cerebral tissue of rats after focal cerebral ischemia and reperfusion (I/R).The local cerebral I/R model was established by ligation of the middle cerebral arteries (MCA). The animals were divided into the sham-operative group, the model group, the Yiqi Huoxue Recipe (YHR) group, the Zhengan Xifeng Decoction (ZXD) group and the Xinglou Chengqi Decoction (XCD) group. The mitochondria in brain tissue was obtained by density-centrifugation and differential centrifugation, then the activities of succinate dehydrogenase (SDH), Na+ -K+ -ATPase, creatine kinase-BB (CK-BB) in homogenate of brain tissue were measured by chemical chromometry.Activities of SDH and Na+-K+ -ATPase were lower and that of CK-BB was higher in the model group than those in the sham -operative group at all time points after I/R (P0.01). Compared with those in the model group, activity of Na+ -K+ -ATPase was higher only in the ZXD group at 24 h after I/R, while at 48 h and 72 h after I/R, activities of both SDH and Na+ -K+ -ATPase were higher in all the treatment groups. As for the activity of CK-BB, it was lower in all the treatment groups (P0.05). The optimal effect was shown in the ZXD group at 24 h, in the XCD group at 48 h, and in the YHR group at 72 h after I/R.The three traditional Chinese medicinal compounds could reduce pathologic injury after focal cerebral I/R in rats by promoting activity of SDH and Na+ -K+ -ATPase and inhibiting that of CK-BB, the optimal effect of ZXD was shown at 24 h after I/R, that of XCD at 48 h after I/R and of YHR at 72 h after I/R.