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Abstract This study aims to evaluate the prognostic utility of Activity-dependent neuroprotective protein (ADNP) expression in Circulating Tumor Cells (CTCs) inpatients with Non-muscle-invasive Bladder Cancer (NMIBC) undergoing Transurethral Resection of Bladder Tumor (TURBT). A prospective cohort of 74 bladder cancer patients and 22 non-cancer controls were enrolled. The expression of ADNP mRNA was detected by immunomagnetic beads-droplet digital PCR. The ADNP mRNA expression was evaluated in patients with high-risk NMIBC and those with indeterminate invasion depth post 2nd TURBT. Primary cultured bladder cancer cells and PBMCs from healthy donors were immunofluorescence stained. Our findings suggest that baseline ADNP mRNA level in CTCs shows potential as a prognostic marker for NMIBC with a sensitivity of 83.33% and a specificity of 73.58%. In comparison to baseline, ADNP mRNA expression increased post 2nd TURBT in 5 patients, where 2 experienced recurrence. Meanwhile, among the 12 patients with decreased levels, only one patient relapsed. A considerable limitation of this study entails the small sample size. The Immuno-magnetic beads-ddPCR technique provided a viable method for ADNP mRNA detection in CTCs from bladder cancer patients. The preoperative ADNP mRNA level in CTCs was identified as a prognostic indicator for NMIBC. Longitudinal monitoring of ADNP mRNA in CTCs of bladder cancer patients shows promise in evaluating treatment responses and predicting prognosis.

Abstract Background In patients undergoing total joint arthroplasty (TJA), the administration of dexamethasone may contribute to perioperative blood glucose (BG) disturbances, potentially resulting in complications, even in patients without diabetes. This study aimed to demonstrate the impact of different administration regimens of dexamethasone in postoperative BG levels. Methods In this randomized, controlled, double-blind trial, 136 patients without diabetes scheduled for TJA were randomly assigned to three groups: two perioperative saline injections (Group A, placebo); a single preoperative injection of 20 mg dexamethasone and a postoperative saline injection (Group B), and two perioperative injections of 10 mg dexamethasone (Group C). Primary outcomes were the postoperative fasting blood glucose (FBG) levels. Secondary outcome parameters were the postoperative postprandial blood glucose (PBG) levels. Postoperative complications within 90 days were also recorded. Risk factors for FBG ≥ 140 mg/dl and PBG ≥ 180 mg/dl were investigated. Results Compared to Group A, there were transient increases in FBG and PBG on postoperative days (PODs) 0 and 1 in Groups B and C. Statistical differences in FBG and PBG among the three groups were nearly absent from POD 1 onward. Both dexamethasone regimens did not increase the risk for postoperative FBG ≥ 140 mg/dl or PBG ≥ 180 mg/dl. Elevated preoperative HbA1c levels may increase the risk of postoperative FBG ≥ 140 mg/dl or PBG ≥ 180 mg/dl, respectively. Conclusion Perioperative intravenous high-dose dexamethasone to patients without diabetes has transient effects on increasing BG levels after TJA. However, no differences were found between the split-dose and single high-dose regimens. The elevated preoperative HbA1c, but not the dexamethasone regimens were the risk factor for FBG ≥ 140 mg/dl and PBG ≥ 180 mg/dl. Trial registration Chinese Clinical Trail Registry, ChiCTR2300069473. Registered 17 March 2023, https://www.chictr.org.cn/showproj.html?proj=186760 .

Background:. With the accumulation of experience and evidence, guidelines for invasive intervention for acute pancreatitis (AP) have continuously evolved, followed in Peking Union Medical College Hospital (PUMCH). We aimed to review AP case series to help understand the impact of guideline evolution on the management and prognosis of AP in real-world practice. Methods:. This was a single-center study of AP patients who had received invasive interventions from 1988 to 2022. Patient demographics, baseline severity, imaging findings, and the indication, timing, and specific modalities of invasive interventions were collected. The composite primary endpoint was death during hospitalization or major complications after intervention. Other endpoints included pancreatic fistula, incision infection, number of interventions, length of intensive care stay, length of hospitalization, and total medical cost. Results:. A total of 195 patients were included. The most common indication for invasive intervention was suspected infection, followed by persistent symptoms. The step-up and delayed strategies reduced the incidence of major complications or death. Over 35 years, the number of patients requiring surgery has gradually declined, and more patients need only minimally invasive procedures for remission. The incidence of the primary outcome decreased as well as the duration of hospitalization. Conclusions:. The management of AP patients at PUMCH in the last 35 years has undergone therapeutic guideline changes that support the efficacy and safety of the deferred step-up strategy in real-world practice.

This study introduces a brazing technique for joining Al0·3CoCrFeNi high-entropy alloy (HEA) utilizing a FeCoCrNiCu/Ti composite interlayer. The primary objectives of this investigation encompass an examination of the microstructure of the Al0·3CoCrFeNi joint, as well as an evaluation of the shear strength of the brazed joints under diverse brazing parameters. The characteristic microstructure of the Al0·3CoCrFeNi brazing seam is comprised of Ti (s, s), Cu (s, s) from the FeCoCrNiCu interlayer, and face-centered cubic (FCC) phases containing more than five elements. When the brazing temperature increases from 1100 °C to 1180 °C, there is a gradual augmentation in the diffusion of Ti element and subsequent reactions involving Cu, Ti, and Ni. Furthermore, the impact of holding time on the brazing seam is investigated. The findings suggest that prolonging the holding time has a relatively negligible effect on the reaction layer formed by the Ti foil. However, it does lead to a reduction of the Cu (s, s) content within the FeCoCrNiCu interlayer. Notably, the highest shear strength reaches up to 280 MPa when the joint brazed at 1160 °C for 10 min. In addition, the shear strength did not decrease below 800 °C. During the shear test, ductile fractures are observed in the joint, indicating favorable mechanical properties. Overall, this study successfully demonstrates the potential of the proposed brazing technique employing a FeCoCrNiCu/Ti composite interlayer for the effective joining of Al0·3CoCrFeNi HEA. By optimizing the brazing parameters, it is possible to achieve robust joints with exceptional mechanical characteristics.

Narrow gap oscillating laser welding with filler wire is a promising technique for joining high-strength steels in construction machinery. In order to investigate the influence of beam oscillation on the surface morphology and microstructure of weld joints of two types of commonly-used high-strength steels, a comparative study between oscillation welding and non-oscillation welding was conducted. High-speed photography technique was employed to reveal the microstructure and mechanical properties of the dissimilar joints. The results showed that laser beam oscillation produces two effects: one was to enlarge the heated surface of molten pool, while the other was to stir the liquid metal in molten pool periodically. Under the first effect, the fusion between sidewall and weld bead layers was promoted, while under the latter, coarse columnar grains ceased to appear, but finer grains were formed. The weld joints between Q355 and 35 steels exhibited excellent tensile impact bending performance, which was attributed to the grain refinement effect. The results also demonstrated that the oscillation welding with a contact angle less than 50° could avoid lack of fusion in multi-pass wire-filling laser welding. The influence of the oscillation welding process on the quality of welding was further discussed.

Carbon isotopes have been used extensively in tracing the sources of oil. However, primary source facies and secondary alteration controls on oil isotopic compositions have not been well resolved, resulting in application uncertainties. A case study was undertaken for an alkaline lacustrine oil system in a lower Permian formation in the Junggar Basin, NW China. Results indicate that increasing maturity causes the carbon isotopic composition to become heavier for only short–middle-chain compounds, whereas source facies-related carbon assimilation controls the compositions of short-, middle-, and long-chain compounds. In particular, light-carbon assimilation during organic-matter degradation makes the isotopic composition lighter, whereas heavy carbon from the water mass makes it heavier. Accordingly, oils in this study area were divided into Type U and Type N oils based on individual compound carbon isotopic compositions, reflecting the difference in source facies in a highly saline and reducing stratified water environment. The results provide a better understanding of the controls on carbon isotopes in oil in sedimentary basins, reducing the uncertainty in oil–source correlation and addressing the origin of oil.

Abstract The recently‐observed poleward shift in western North Pacific tropical cyclone (TC) genesis has increased the TC threat to East Asia. We find that the poleward shift of TC genesis since 1979 is linked to mega‐ENSO. A downscaling analysis of TC genesis latitude given the constraint of mega‐ENSO using 30 models from the Coupled Model Intercomparison Project Phase 6 (CMIP6) show a continued increasing poleward shift with additional warming. We use the dynamic genesis potential index as a TC proxy in future CMIP6 simulations. These simulations show enhanced TC formation in the subtropics and decreased TC formation in the tropics. Modeled TCs in CMIP6 high‐resolution models that well represent mega‐ENSO project future poleward shifts in TC genesis. Both observations and simulations show that extra‐tropical North Pacific sea surface temperature warming associated with mega‐ENSO are the primary driver of the TC genesis poleward shift. Our study provides new insights into climate change‐driven TC migration.

BackgroundIncreasing evidence reveals the involvement of mitochondria and macrophage polarisation in tumourigenesis and progression. This study aimed to establish mitochondria and macrophage polarisation-associated molecular signatures to predict prognosis in gastric cancer (GC) by single-cell and transcriptional data.MethodsInitially, candidate genes associated with mitochondria and macrophage polarisation were identified by differential expression analysis and weighted gene co-expression network analysis. Subsequently, candidate genes were incorporated in univariateCox analysis and LASSO to acquire prognostic genes in GC, and risk model was created. Furthermore, independent prognostic indicators were screened by combining risk score with clinical characteristics, and a nomogram was created to forecast survival in GC patients. Further, in single-cell data analysis, cell clusters and cell subpopulations were yielded, followed by the completion of pseudo-time analysis. Furthermore, a more comprehensive immunological analysis was executed to uncover the relationship between GC and immunological characteristics. Ultimately, expression level of prognostic genes was validated through public datasets and qRT-PCR.ResultsA risk model including six prognostic genes (GPX3, GJA1, VCAN, RGS2, LOX, and CTHRC1) associated with mitochondria and macrophage polarisation was developed, which was efficient in forecasting the survival of GC patients. The GC patients were categorized into high-/low-risk subgroups in accordance with median risk score, with the high-risk subgroup having lower survival rates. Afterwards, a nomogram incorporating risk score and age was generated, and it had significant predictive value for predicting GC survival with higher predictive accuracy than risk model. Immunological analyses revealed showed higher levels of M2 macrophage infiltration in high-risk subgroup and the strongest positive correlation between risk score and M2 macrophages. Besides, further analyses demonstrated a better outcome for immunotherapy in low-risk patients. In single-cell and pseudo-time analyses, stromal cells were identified as key cells, and a relatively complete developmental trajectory existed for stromal C1 in three subclasses. Ultimately, expression analysis revealed that the expression trend of RGS2, GJA1, GPX3, and VCAN was consistent with the results of the TCGA-GC dataset.ConclusionOur findings demonstrated that a novel prognostic model constructed in accordance with six prognostic genes might facilitate the improvement of personalised prognosis and treatment of GC patients.

In recent years, new energy vehicles (NEVs) have taken the world by storm. A large number of NEV batteries have been scrapped, and research on NEV battery recycling is important for promoting the sustainable development of NEVs. Battery recycling is an important aspect of the sustainable development of NEVs. In this study, we conducted an in-depth analysis of the current status of research on NEV battery recycling from a new perspective using bibliometric methods and visualization software. This study shows that research targeting the recycling of NEV batteries is growing rapidly, and collaborative networks exist among researchers from different countries, institutions, and fields. The focus of research has shifted from lead-acid batteries to lithium batteries, and the supply chain and circular economy related to NEV battery recycling is an emerging research hotspot. Based on our analysis, we propose that the government should establish policies to improve the recycling networks at the collection stage and provide subsidies to attract consumers. Enterprises should develop low-cobalt and cobalt-free technologies, utilize green solvents, and develop new battery swap modes. The establishment of an information platform is conducive to the further development of collaborative networks.

Reactive air brazing (RAB) has been widely applied in fabricating solid oxide fuel cell stacks, gas separators, sensors, and other fields due to the joint good oxidation resistance. Nevertheless, a methodical analysis of fundamental principles, development process, and application scope has yet to be reported since RAB was proposed in 2000. Although various braze systems have been developed, a comprehensive comparison and classification are still absent, making it difficult to guide the selection of RAB braze. Besides, this technique faces several intractable issues, such as excessive interfacial reactions, large thermal mismatch, etc., seriously restricting the RAB application. Hence this review elucidates the basic information, braze systems, existing issues, and corresponding solutions of RAB. The joining mechanism, development history, and application fields are explicitly introduced. Reported braze systems are analyzed in-depth and divided into three categories according to reactivity. Further, we outline four issues of RAB: excessive interfacial reaction, excessive thermal expansion coefficient of braze, limited aging performance, and high-temperature softening of braze, and discuss the corresponding solutions. Finally, our review proposed several potential improvements and possible future research trends in the RAB technique, aiming to guide the future development of RAB and offer new insights to broaden its application range.

Abstract Background Bladder cancer (BC) is one of the most common malignancies of the genitourinary system. Phosphofructokinase 1 (PFK-1) is one of member of PFK, which plays an important role in reprogramming cancer metabolism, such as lactylation modification. Zinc finger E-box-binding homeobox 1 (ZEB1) has been demonstrated to be a oncogene in many cancers. Therefore, this study was performed to explore the effects of PFK-1 on the lactylation of ZEB1 in BC development. Methods Cell viability was measured using the CCK-8 kit. The glucose assay kit and lactate assay kit were used to detect glucose utilization and lactate production. The DNA was purified and quantified by qRT-PCR. Results In the present study, we found that ZEB1 expression levels were significantly elevated in bladder cancer cells. Impaired PFK-1 expression inhibits proliferation, migration, and invasion of BC cells and suppresses tumour growth in vivo. We subsequently found that knockdown of PFK-1 decreases glycolysis, including reduced glucose consumption, lactate production and total extracellular acidification rate (ECAR). Mechanistically, PFK-1 inhibits histone lactylation of bladder cancer cells, and thus inhibits the transcription activity of ZEB1. Conclusion Our results suggest that PFK-1 can inhibit the malignant phenotype of bladder cancer cells by mediating the lactylation of ZEB1. These findings suggested PFK-1 to be a new potential target for bladder cancer therapy.

Abstract Chemotherapeutic drugs will affect the process of spermatogenesis. However, most current studies on the effects of chemotherapeutic drugs on spermatogenesis are based on mouse models, with a shortage of human body evidence. In addition, the mechanism of chemotherapeutic drugs causing spermatogenesis disorder is not clear. Therefore, we have collected the testicular tissues of an inguinal-lipoma patient whose testes were resected after chemotherapy and a patient who had normal spermatogenesis disorder and underwent single-nucleus RNA sequencing (snRNA-Seq). After quality control, we obtained a total of 27,957 high-quality cells, including 18,612 normal cells and 9,345 drug-treated cells, which were all used in analyzing the mechanism of chemotherapeutic drugs causing spermatogenesis disorder. This study has provided data resources and references for exploring the mechanism of chemotherapeutic drugs causing spermatogenesis disorder with the insight of protecting the spermatogenic abilities of male tumor patients receiving chemotherapy.

Abstract Implant-associated infections due to the formation of bacterial biofilms pose a serious threat in medical healthcare, which needs effective therapeutic methods. Here, we propose a multifunctional nanoreactor by spatiotemporal ultrasound-driven tandem catalysis to amplify the efficacy of sonodynamic and chemodynamic therapy. By combining piezoelectric barium titanate with polydopamine and copper, the ultrasound-activated piezo-hot carriers transfer easily to copper by polydopamine. It boosts reactive oxygen species production by piezoelectrics, and facilitates the interconversion between Cu2+ and Cu+ to promote hydroxyl radical generation via Cu+ -catalyzed chemodynamic reactions. Finally, the elevated reactive oxygen species cause bacterial membrane structure loosening and DNA damage. Transcriptomics and metabolomics analysis reveal that intracellular copper overload restricts the tricarboxylic acid cycle, promoting bacterial cuproptosis-like death. Therefore, the polyetherketoneketone scaffold engineered with the designed nanoreactor shows excellent antibacterial performance with ultrasound stimulation and promotes angiogenesis and osteogenesis on-demand in vivo.

Objectives The objective of this study was to quantify the morphology, composition, and asymmetry of the paravertebral extensor muscles (PSEMs) in patients with cervical ossification of the posterior longitudinal ligament (OPLL) who had different modified K‐line (mK‐line) and the minimum interval between the mK‐line and OPLL (INTmin) values and to investigate the relationship between PSEMs and symptoms and outcomes following laminoplasty. These original findings elucidated that the atrophy of PSEMs could predict decompression outcomes and provided a theoretical basis for paraspinal muscle rehabilitation. Methods A total of 94 consecutive patients who underwent laminoplasty for OPLL between January 2020 and January 2022 were enrolled in this retrospective study. The relative cross‐sectional areas (rCSA), functional cross‐sectional areas (rFCSA), and FCSA/CSA ratio of the multifidus (MF), semispinalis cervicalis (SSCe), semispinalis capitis (SSCa), and splenius capitis (SpCa) were measured at the C3–C7 segments on cervical magnetic resonance imaging (MRI). This study compared the differences between the mK‐line (+) group and the mK‐line (−) group, as well as between the INTmin

In the paper, the authors simply review recent results of inequalities, monotonicity, signs of determinants, determinantal formulas, closed-form expressions, and identities of the Bernoulli numbers and polynomials, establish an identity involving the differences between the Bernoulli polynomials and the Bernoulli numbers, present two identities among the differences between the Bernoulli polynomials and the Bernoulli numbers in terms of a determinant and a partial Bell polynomial, and derive a determinantal formula of the differences between the Bernoulli polynomials and the Bernoulli numbers.

Vacuum brazing of TiAl alloy is achieved using TiZrNiCu fillers and BNi-2 fillers. The oxidation behavior of the two kinds of TiAl alloy joints is investigated in air at 800 °C. The influence of high-temperature oxidation on the microstructure and mechanical properties of the joints are characterized by scanning electron microscopy, X-ray diffraction, transmission electron microscopy, and shear tests. It is found that long-term oxidation does not have a large effect on the microstructure of joints. However, the fillet of the brazed joint achieved via BNi-2 fillers forms longitudinal solidification cracks, which proved that the brazed joint using TiZrNiCu fillers shows more excellent oxidation resistance.

Glass/Glass-ceramic sealing is always accompanied by issues of high porosity and brittle joints. To migrate these problems, an Ag foam was innovatively induced as an interlayer in the process of glass sealing the yttria-stabilized zirconia (YSZ) ceramic to Mn–Co spinel-coated Crofer 22 H stainless steel. Interlayer-added joints showed a significant reduction in porosity, and the shear strength was improved to 52.3 MPa, with an enhancement of 37.8 %. The influence of interlayer on the microstructure of the joint has been investigated and the formation process of the joint is illustrated. The Ag interlayer expels gases from the sealant material through expansion at high temperatures and promotes finer nucleation grains of crystallites integrating with the interlayer, reducing the porosity and alleviating the brittleness as well.

BackgroundIschaemic heart failure with reduced ejection fraction (HFrEF) caused by coronary artery disease accounts for the largest proportion of heart failure cases with the worst prognosis. Coronary artery bypass grafting (CABG) is the most effective treatment for ischaemic HFrEF. On-pump and off-pump are the two surgical methods used for CABG. Whether patients with HFrEF should undergo on- or off-pump CABG is controversial in coronary heart disease surgery. The left ventricular end-systolic volume index (LVSEVI) is the gold standard for evaluating the severity of left ventricular remodelling; however, its effect on the perioperative risk and long-term survival rate of patients with HFrEF undergoing CABG remains unclear.MethodsThis single centre prospective cohort analysis included 118 coronary heart disease patients with symptoms and signs of heart failure and a left ventricular ejection fraction (LVEF) of

Abstract Background Ovarian cancer (OC) causes the highest rates of mortality among women’s genital tract malignancies. Micro-ribonucleic acid (miRNA), the most abundant long noncoding RNAs transmitted by exosomes, has been revealed to be a potential marker for OC since 2008. In this study, we aimed to determine the possible roles of miRNAs derived from exosomes in the early diagnosis of OC through miRNA microarray, besides, exploring the underlying mechanisms of miRNAs in the OC progression. Methods We isolated exosomes from high invasive OC cell line HO8910PM and its parent cell line HO8910 using transmission electron microscopy and western blot, and performed miRNA microarray to identify the exosome-transmitted miRNA from the two cell lines, respectively. The expression profile was obtained by quantitative analysis, and then the differentially expressed individuals were screened. miRNA-30a-5p, a stable miRNA in both cells of our sequencing data was set for further study. MiR-30a-5p mimics, inhibitor and their corresponding negative controls were applied in OC cells. Then the cell proliferation, migration, and invasion of different groups were analyzed via cell counting-kit 8 (CCK8), wound healing, and Transwell analyses. Besides, ZBE2 and LDH2 expressions were detected by qRT-PCR. Results Combined with the data report of miRNA microarray technology, we set miR-30a-5p as our target miRNA to analyze its molecular function in regulating proliferation, migration, and invasion in OC cells. Our results showed that the miR-30a-5p overexpression could significantly enhance the capability of proliferation, migration, and invasion of HO8910 and HO8910PM cells, whereas the miR-30a-5p inhibition showed the opposite tendency (all P

Laminated composites have been extensively researched due to their excellent mechanical properties, but their application in superwetting fields has been rarely reported. In this work, the surfaces with extreme wettability were prepared based on laminated composites. The Ti6Al4V/304 stainless steel (304 SS) micro-laminated composites were fabricated by diffusion bonding the Ti6Al4V foils and 304 SS foils with the thickness of 10–50 μm in vacuum. The typical interfacial microstructure and the effect of diffusion temperature on the microstructure were investigated. The bonded interface of joints is mainly composed of β-Ti, Ti2Ni, FeTi, Fe2Ti, Fe–Cr from the Ti6Al4V side to the 304 SS. The effect of diffusion temperature on the microstructure was researched. Based on the prepared Ti6Al4V/304 SS micro-laminated composites, an array structure with micro grooves was fabricated through the simple chemical etching. The 304 SS foils were etched to form grooves, while Ti6Al4V foils still remained as bulges. Due to surface modification and rough morphology, the etched surfaces exhibited the excellent hydrophobicity. Increasing the width and depth of grooves and decreasing the width of bulges led to the higher water contact angles and improved hydrophobicity. The highest water contact angles of 148.3° has been achieved under the conditions of grooves with a width of 50 μm, a depth of 60 μm, and the bulges with a width of 10 μm. The surfaces exhibited nearly superhydrophobicity and performed self-cleaning property. The processing method of this work could expand the application of laminated composite in extreme wettability fields.

K418 alloy was prepared by selective laser melting (SLM) at different laser powers (160∼240 W) in this paper. The influence of laser power on the densification behavior, microstructure, microhardness, and tensile properties was studied systematically. The results showed that the densities of the material increased rapidly first and then decreased slowly with the increase of laser power. When the laser power increased from 160 W to 240 W, the surface defects of the specimen decreased, the microstructure changed from columnar dendrites to cellular, and the grains grew in the direction preferentially. When the laser power is 200 W, the grain size is the smallest, the content of small-angle grain boundaries is more, the Schmidt factor is less, and then the best mechanical properties are achieved, at which the microhardness reaches 362.89 ± 5.01 HV, the tensile strength reaches 1244.35 ± 70.1 MPa, the yield strength reaches 863.89 ± 23.1 MPa, and the elongation of the material reaches a maximum of 12.53 ± 0.71 %. In addition, the fracture mode of the material is a mixed tough-brittle fracture mechanism.

Abstract Based on the mining conditions of shallow close coal seams in the Jurassic coalfield of northern Shaanxi, in order to explore the evolution and development height prediction of water-conducting fracture zone (WCFZ), combining with measured statistical analysis, physical simulation, numerical calculation, and theoretical analysis, the evolution of mining-induced fractures and the development characteristics of WCFZ are analyzed, and the development height predicting method of WCFZ in shallow close coal seams mining is proposed. The results show that in shallow single coal seam mining, during the stage of incritical mining, the height of the WCFZ increases in a “jumping” pattern with the main roof periodic caving. After critical mining, it is basically stable. Its development height is positively correlated with the mining height. The ratio of development height to mining height is generally 20–30, and the change is not significant with the mining height, overall, the distribution of the WCFZ is “saddle shaped”. Based on close distance coal seams repeated mining, the primary fractures act as activation development when the interburden broken completely, the development of WCFZ at the mining boundary is significantly intensified, and ratio of the height to mining height in repeated mining is obviously lower than that in single seam mining, which is generally 18–25. The traditional empirical formula cannot be applied to the development prediction in shallow close coal seams mining, with an error of 10–50%. Considering the mining height, interburden thickness, and roof caving characteristics, the development height predicting method of WCFZ in shallow close coal seams mining is proposed. Compared with measured results, the calculation error is basically within 15%, which has good prediction accuracy, The research results can provide a theoretical basis for predicting the height of WCFZ in shallow close coal seams mining in the Jurassic coalfield of northern Shaanxi.

The prolonged duration of phytoremediation poses a risk of heavy metal dispersal to the surrounding environment. This study investigated a combined remediation approach for cadmium (Cd)- and arsenic (As)-contaminated soil by integrating phytoremediation with stabilization techniques. Bidens pilosa was utilized as the phytoremediator, and steel slag, pyrolusite, and FeSO4 were employed as stabilizing agents in the pot experiments. Key metrics such as soil moisture content, root length, plant height, and heavy metal concentrations in Bidens pilosa were measured to evaluate the remediation efficacy. Additionally, the bioavailability, leaching toxicity, and chemical forms of Cd and As, along with other soil properties, were analyzed. The results indicated that the optimal restoration effect was achieved by combining steel slag, pyrolusite, and FeSO4 with stabilizers in a ratio of 2:1:10. Additionally, the optimal dosage of these materials was found to be 9% by weight. Mechanistic studies, including heavy metal speciation analysis, X-ray photoelectron spectroscopy (XPS), and microbial community diversity analysis, revealed that the stabilization effects were primarily due to the interactions of anionic and cationic ions, chelation by organic acids secreted by plant roots, and enhanced microbial activity. A cost–benefit analysis demonstrated the technical, economic, and commercial viability of the combined remediation approach.

Pesticide residues in soil, especially multiple herbicide residues, cause a series of adverse effects on soil properties and microorganisms. In this work, the degradation of three herbicides and the effect on bacterial communities under combined pollution was investigated. The experimental results showed that the half-lives of acetochlor and prometryn significantly altered under combined exposure (5.02–11.17 d) as compared with those of individual exposure (4.70–6.87 d) in soil, suggesting that there was an antagonistic effect between the degradation of acetochlor and prometryn in soil. No remarkable variation in the degradation rate of atrazine with half-lives of 6.21–6.85 d was observed in different treatments, indicating that the degradation of atrazine was stable. 16S rRNA high-throughput sequencing results showed that the antagonistic effect of acetochlor and prometryn on the degradation rate under combined pollution was related to variation of the Sphingomonas and Nocardioide. Furthermore, the potential metabolic pathways of the three herbicides in soil were proposed and a new metabolite of acetochlor was preliminarily identified. The results of this work provide a guideline for the risk evaluation of combined pollution of the three herbicides with respect to their ecological effects in soil.

As an emerging direction of multi-agent collaborative control technology, multiple autonomous underwater vehicle (multi-AUV) cooperative area search technology has played an important role in civilian fields such as marine resource exploration and development, marine rescue, and marine scientific expeditions, as well as in military fields such as mine countermeasures and military underwater reconnaissance. At present, as we continue to explore the ocean, the environment in which AUVs perform search tasks is mostly unknown, with many uncertainties such as obstacles, which places high demands on the autonomous decision-making capabilities of AUVs. Moreover, considering the limited detection capability of a single AUV in underwater environments, while the area searched by the AUV is constantly expanding, a single AUV cannot obtain global state information in real time and can only make behavioral decisions based on local observation information, which adversely affects the coordination between AUVs and the search efficiency of multi-AUV systems. Therefore, in order to face increasingly challenging search tasks, we adopt multi-agent reinforcement learning (MARL) to study the problem of multi-AUV cooperative area search from the perspective of improving autonomous decision-making capabilities and collaboration between AUVs. First, we modeled the search task as a decentralized partial observation Markov decision process (Dec-POMDP) and established a search information map. Each AUV updates the information map based on sonar detection information and information fusion between AUVs, and makes real-time decisions based on this to better address the problem of insufficient observation information caused by the weak perception ability of AUVs in underwater environments. Secondly, we established a multi-AUV cooperative area search system (MACASS), which employs a search strategy based on multi-agent reinforcement learning. The system combines various AUVs into a unified entity using a distributed control approach. During the execution of search tasks, each AUV can make action decisions based on sonar detection information and information exchange among AUVs in the system, utilizing the MARL-based search strategy. As a result, AUVs possess enhanced autonomy in decision-making, enabling them to better handle challenges such as limited detection capabilities and insufficient observational information.

Despite the lengthy history of the research on destination image from various perspectives, how pro-environmental destination image promotes resident environmental citizenship behavior remains underexplored. Grounded in the cognition–affect–behavior (CAB) model, this research investigated the translation of pro-environmental destination image into resident environmental citizenship behavior via satisfaction and pride. Data were collected using an intercept survey approach from a tourism village recognized as one of the best in the world by UNWTO. The results indicated that a pro-environmental destination image has a positive impact on resident environmental citizenship behavior in the private and public domains. Furthermore, resident satisfaction and pride serve as mediators between pro-environmental destination image and resident environmental citizenship behavior. This research contributes to the literature on resident environmental citizenship behavior by considering the role of pro-environmental destination image. The findings provide practical implications for fostering environmental citizenship behavior through the presentation of pro-environmental images to residents and eliciting their positive emotions.