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Imported Coronavirus disease 2019 (COVID-19) patients pose a huge challenge to the prevention and control of the epidemic in prefecture-level cities in China. However, the treatment strategies at that time were mainly empirical and far from perfect. Hence, this study aims to summarize the clinical characteristics, diagnosis and treatment of all COVID-19 patients in Jiaxing City in 2020.The clinical data of 42 patients diagnosed with COVID-19 in Jiaxing City, Zhejiang Province from January 23, 2020 to March 4, 2020 were retrospectively analyzed. Epidemiological history and sociodemographic data were collected. Laboratory parameters, imaging and disease progression, treatment methods, efficacy and adverse reactions of COVID-19 cases were recorded. Then, the clinical characteristics as well as diagnosis and treatment were statistically analyzed.The median age of 42 patients was 47 years old, including 24 males (57.1%) and 18 females (42.9%). There were 21 first-generation cases, 29 cases (69%) of clustering onset related to first-generation cases, and 28 cases without any underlying diseases. Radiographic progression was reported in 17 patients (40.5%) (progression duration, 2-11 days; median progression duration, 3.8 days; average progression duration, 4.59-2.48 days). The main clinical symptoms include fever (78.6%) and cough (64.3%). A total of 37 patients (88.10%) received arbidol combined with lopinavir/ritonavir or darunavir/cobicistat. Of these, 22 patients (52.4%) took a combination with moxifloxacin tablets, and 20 patients (47.6%) took combined hormone therapy. Seventeen patients (40.48%) reported diarrhea, nausea, vomiting, rash, and other adverse drug reactions. A total of 38 patients improved (90.5%). The hospital stays of 36 patients ranged from 7 to 33 days, with a median of 19 days (19.00-7.33 days on average). The virus nucleic acid test result return time was 1 to 32 days, with a median of 15.5 days (14.41-8.61 days on average).Most of the imported COVID-19 patients in Jiaxing City were of the first generation, mainly cluster onset, and the epidemiological characteristics were relatively simple. Arbidol combined with lopinavir/ritonavir or darunavir/cobicistat was the main treatment strategy for the initial treatment of COVID-19.

Purpose We conducted this meta-analysis to compare postoperative outcomes between meatal-based flap (Mathieu) and tubularized incised plate (TIP) techniques for distal hypospadias. Methods A comprehensive literature search of PUBMED, Web of Science, EMBASE, and Cochrane Library was conducted. Outcomes evaluated in this review were fistula, meatal stenosis, wound dehiscence and flap necrosis. We calculated odds ratio (OR) with 95% confidential interval (CI) to compare postoperative outcomes between Mathieu and TIP after data extraction and literature identification. All data were analyzed using Review Manager 5.2. In order to find potential affective factors, meta-regression and subgroup analyses were applied. Results 16 studies, 1386 patients, including 762 patients receiving Mathieu and 624 individuals subjected to TIP met the inclusion criteria. The synthetic data suggested that Mathieu and TIP were comparable in terms of fistula (OR = 0.93; 95% CI: 0.65 to 1.33; P = 0.70, I2 = 14%), wound dehiscence (OR = 0.89; 95% CI: 0.33 to 2.39; P = 0.81, I2 = 11%), and flap necrosis (OR = 1.9; 95% CI: 0.51 to 7.09; P = 0.20, I2 = 38%) without significant heterogeneity for each comparison group. Pooled estimates showed a significantly lower rate of meatal stenosis with Mathieu than with TIP (OR = 0.41; 95% CI: 0.24 to 0.73; P = 0.002, I2 = 4%). Subgroup analyses showed that the difference between Mathieu and TIP was more obvious in the studies published before 2013 in meatal stenosis. The modified Mathieu technique and a running suture for urethroplasty might be relevant to a lower rate of meatal stenosis in the data, although no statistical significance in the present effects model overall was found. One-way sensitivity analysis showed that the results were stable. There was no publication bias detected using both funnel plot and Egger's test. Conclusion This meta-analysis suggested that Mathieu and TIP technique were equivalent for primary distal hypospadias in terms of fistula, wound dehiscence, and flap necrosis. Pooled estimates indicated that there was a lower rate of meatal stenosis with Mathieu rather than with TIP significantly. The modified Mathieu technique and a running suture for urethroplasty might be relevant to a lower rate of meatal stenosis. Type of study Meta-analysis. Level of evidence IV

The formation of Section B, which is on the right bank of the Amu Darya consist of high pressure salt and gypsum formation. The average thickness of the salt-gypsum layer is about 900 meters, the maximum pressure coefficient is 2.3 g/cm3, the salt rock is easy to creep, resolve, and crystallize. The salt layer is close to the reservoir. Wellbore path is difficult to control. The drilling accident rate is 34%, and the success rate of high pressure brine drilling is 0, which leads to the fact that no horizontal well drilling has been carried out. In order to develop the safety technology, it is necessary to carry out the large angle orientation drilling method in the high-pressure salt water and salt-gypsum layer. However, this kind of method has not been widely used, so it is urgent to solve the safe drilling problem and orientation problem of the high pressure salt paste layer. The proper proportion of drilling mud in the salt-gypsum layer is optimized by establishing the bore shrinkage equation and using the layer control method to determine the pressure of the salt-gypsum layer. After that, the unsaturated saturated brine drilling fluid system compatible with the salt rock layer is selected. After the salt-gypsum layer is sealed with casing tube, the reservoir will be re-drilled. The slope will be designed in the salt layer, and the tricone bit control will be carried out by adding the cone bit and selecting the MWD directional drilling tool combination. The proper density of the well drilling mud will prevent the salt rock from creeping and high pressure salt water from flooding. The stable saturated brine drilling mud will then be controlled effectively, and the stability of the well bore caused by salt losing is effectively controlled. The well bore expansion rate dropped from 19% to 2%; with salt-gypsum layer is sealed with 250.8mm thick anti-extrusion casing, and the water expansion packer is installed, which effectively prevent high pressure salt water channeling and casing tubes collapsing. After that, it is easy to control the drill direction angel in the high-pressure salt gypsum formation between 0° and 80°. After putting the directional drilling tool in the centralizer position and combined with other skills, the drilling speed has been increased by 8.7%. Up till now, the horizontal drilling technology has been widely used in the zone. 50 wells have been using this drilling technology and goes on well.

To construct and screen short hairpin RNA (shRNA) targeting vascular endothelial growth factor (VEGF), and investigate potential values of VEGF-shRNA on angiogenesis and growth in renal cell carcinoma (RCC) in a xenograft tumor model. VEGF-shRNA fragment was designed to connect plasmid vector, and RCC cells were transfected with shRNA. Real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR) was used to detect interference efficiency of VEGF gene. The xenograft tumor model was established in nude mice, and mice were randomly divided into blank control (BC) group, negative control (NC) group, and experimental group. RNA interference (RNAi) effect was detected by immunohistochemistry, and tumor volume changes were observed. Tumor-bearing nude mice model was established and mice were randomly divided into BC group, NC group, and treatment group. The tumor volume changes and tumor inhibition rate were recorded, and angiogenesis status was observed. The apoptosis of tumor cells and genetic toxicity of VEGF-shRNA were detected. VEGF-shRNA can inhibit VEGF mRNA expression with an inhibition ratio of 72.3%. Compared with NC group and BC group, experimental group presents smaller tumor volume, weight, and poor growth (all p0.05). Positive VEGF rate in experimental group is significantly lower than that in NC group and BC group (all p0.05). Significantly lower tumor volume, less microvessel density (MVD) value, and higher apoptotic index (AI) are found in treatment group compared with BC group and NC group (all p0.05). There was no significant difference in AI between treatment group and BC group regarding adjacent normal tissues (p0.05). VEGF plays an important role in the occurrence and development of RCC, chemical synthesis of VEGF small interfering RNA (siRNA) can specifically inhibit VEGF expression, angiogenesis and growth in RCC, and can promote cell apoptosis without genetic toxicity to normal tissues.

Participatory sensing has been envisioned as a promising paradigm for rapidly sharing of environmental information by mobile smart phones that are always equipped with sensors. It invokes a large amount of mobile applications, e.g., participatory environmental monitoring, transportation and traffic management, and personal entertainments. As participators are usually volunteers, participatory sensing thus poses several fundamental security problems such as data trustworthiness, participator reputation evaluation, participator privacy protection and functional robustness against inner attacks or malicious participators. Although some schemes have already been proposed in recent years, none of them is able to solve the above problems in one solution, especially, in a lightweight manner. In this paper, we make the first attempt in this regard and propose a scheme called LibTip. We pinpoint and model several new attacks in participatory sensing that are launched from inner attackers such as bad-mouth attack and key leakage attack. The definitions for the security of the scheme are formally stated. The security and performance are thoroughly analysed or proved, which justifies the applicability of LibTip.