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A 10-residue, glycosaminoglycan-binding peptide, GBPECP, derived from human eosinophil cationic protein has been recently designated as a potent cell-penetrating peptide. A model system containing peptide, glycan, and lipid was monitored by nuclear magnetic resonance (NMR) spectroscopy to determine the cell-penetrating mechanism. Heparin octasaccharide with dodecylphosphocholine (DPC) lipid micelle was titrated into the GBPECP solution. Our data revealed substantial roles for the charged residues Arg5 and Lys7 in recognizing heparin, whereas Arg3 had less effect. The aromatic residue Trp4 acted as an irreplaceable moiety for membrane insertion, as the replacement of Trp4 with Arg4 abolished cell penetration, although it significantly improved the heparin-binding ability. GBPECP bound either heparin or lipid in the presence or absence of the other ligand indicating that the peptide has two alternative binding sites: Trp4 is responsible for lipid insertion, and Arg5 and Lys7 are for GAG binding. We develope...

The core of this research was to study the amount, diversity, richness, evenness and dominance of soil Actinomycetes at lakeside of swamping wetland. In this study, Amount of Actinomycetes were determined through plate counting method, which was in Phragmites australis, Suaeda salsa and Nitraria tangutorum growing soil at the upstream, midstream and downstream of Wuliangsuhai. Community structure of Actinomycetes were analyzed on the basis of it‘s diversity index (H), evenness index (J), richness index (R) and dominance index (D). The results showed that, the diversity, richness and dominance of Actinomycetes were all affected by the types of plants, and showed significant difference among them. However, there was no significant difference for evenness. What’s more, both the order of diversity and richness in different plants growing soil were: Nitraria tangutorum> Suaeda salsa >Phragmites australis. And the order of dominance was: Suaeda salsa >Phragmites australis >Nitraria tangutorum. Dominant bacteria in Phragmites australis was Flavus, accounting for 85%, dominant bacteria in Nitraria tangutorum were Albosporus and Flavus, which accounted for 54.8% and 26%, and dominant bacteria in Suaeda salsa were Albosporus,Albosporus and Flavus, accounted for 26.7%, 23.5%. As the succession from Phragmites australis swamp to Suaeda salsa Saline meadow, and then to Nitraria tangutorum desert in Wuliangsuhai, the composition and structure of Actinomycetes there are changing at the same time.

This study used plate count method to analyze the composition of the Spore-forming bacteria community in the soil in swamping wetland, alkaline land, and desert belt, all of which grow in the upstream, midstream and downstream of swamping wetland of Wuliangsuhai. In this process, the Shannon-Wiener index, Simpson index were used to analyze the index of diversity (H), the index of evenness (J), the index of richness (R) and the index of dominance (D) of the Spore-forming bacteria community. The results showed that: the diversity index of in soil of the swamping wetland and desert belt was the highest in the midstream, second in the upstream, and lowest in the downstream; that in soil of the alkaline land was the highest in the midstream, second in the downstream and lowest in the upstream, and showed significant differences between in the midstream and downstream and in upstream; that in soil of the desert belt showed significant differences between in the upstream and downstream and in midstream; in the same area, in the desert belt soil of Spore-forming bacteria more than swamping wetland and alkaline land.

The study investigated the vertical distribution of soil microorganism on Caragana rhizosphere at Hobq of ORDOS. The result showed that microbial vertical distribution was obvious. The order of vertical distribution in number of aerobic bacteria were 0-10cm>20-30cm>10-20cm>30-40cm, and there were significant differences between microorganisms in 0-10cm, 10-20cm and 30-40cm underground; the number of aerobic bacteria in 0-10cm underground was higher than 10-20cm, 20-30cm and 30-40cm by 1.48,1.41 and 1.86. The order of vertical distribution in number of fungi were 0-10cm>10-20cm>20-30cm>30-40cm, and there were significant differences between 0-10 cm and 20-30cm、30-40cm, and between 10-20 cm and 20-30cm、30-40cm. the number of fungi in 0-10cm underground was higher than 10-20cm, 20-30cm and 30-40cm by 1.01, 3.60 and 5.37. The order of vertical distribution in number of Actinomycetes was 0-10cm>10-20cm>20-30cm>30-40cm, and the differences between 0-10 cm and 10-20cm, 20-30cm, 30-40cm were significant; the number of Actinomycetes in 0-10cm underground was higher than 10-20cm, 20-30cm and 30-40cm by 1.54,1.66 and 2.60. The distribution and quantity of soil microorganisms might be influenced by organic matter contents.

The high-quality phosphorus-doped ZnO nanocombs were synthesized by the simple chemical vapor deposition method without use of any catalyst. The images of field-emission scanning electron microscopy show that the phosphorus-doped ZnO nanocombs have a uniform length of 8 μm with the diameter of 70 nm and the cusp of the teeth of 20 nm. The measurement results of selected-area electron diffraction pattern indicate that the phosphorus-doped ZnO nanocombs are single crystalline structure. The phosphorus related acceptor emission was observed in the photoluminescence spectra of phosphorus-doped ZnO nanocombs at 11 K. The acceptor binding energy is estimated to be ∼127 meV.

A 10-residue, glycosaminoglycan-binding peptide, GBP

Study on the distributive characteristics of the soil microorganisms at floodplain on wetland of Mongolian plateau. Using the Plate dilution method to determine the 0~10cm, 10~20cm, 20~30cm, 40~50cm and 50~60cm soil samples, soil aerobic bacteria, bacillus type bacteria, fungus and actinomycetes bacteria number, the results show that with the deepening of the soil depth, the number of aerobic bacteria number reduced, the peak value of the number of aerobic bacteria in the 0~10cm soil layer, the minimum number in the 50~60cm soil, 0~10cm and the other four soil there was a significant difference, there was no significant difference between the other four soil layers. The Bacillus type bacteria have a high ratio of total aerobic bacteria in 0~10cm, the surface of the soil with a high ammonification quality but also the best. The peak of the number of fungus in 0~10cm, followed by 10~20cm and 30~40cm and 20~30cm, 40~50cm, 50~60cm no isolated fungus. There is no actinomycetes separation in the 10-3 cfu.g-1dry soil dilution.