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In this article, a novel vertical wire-bonding interconnect structure is intelligently modeled and optimized with general artificial neural network (GRNN) and genetic algorithm (GA) for multilayered system in package. A compensation structure is constructed with a hybrid inductive and capacitive technique, while a capacitive stripline with series inductive short-end via is designed underneath the 50-Ω transmission line. The GRNN algorithm is employed to build the electromagnetic model databases during the procedure of GA optimization. In comparison with the conventional optimization algorithm, the output performances can be directly achieved with collaboratively combined methods, which can significantly reduce the calculation time. From the measurement results, the return loss is improved significantly while the parasitic inductive behavior of the bonding wire is eliminated with the presented design. Moreover, compared with the traditional compensation techniques, no additional area is occupied on the surface plane of the wire-bonding interconnection, which is more suitable for the high-integrated circuit system.

B-Cell lymphoma-2 (Bcl-2) protein is a new promising target for anticancer drugs. A number of anticancer Bcl-2 inhibitors with diverse chemical structures have been discovered in recent years. In this paper, the flexible docking was performed to determine the binding modes of the representative inhibitors from different structural types. Subsequently, the binding modes of inhibitor were used to construct a primary three- dimensional (3D) pharmacophore model. It proved that this model can effectively disrupt the binding of the BH3 domain of proapoptotic Bcl-2 family members to Bcl-2, and match the structural requirement of a new type of Bcl-2 inhibitors. However, these distances between pharmacophoric points are not optimal due to the fact that not all of individual functional groups are located in the ideal position when inhibitors bind to its receptor. In this paper, we proposed a new idea to improve the quality of the pharmacophore model: the multiple copy simultaneous search (MCSS) method was performed to determine the energetically favorable distribution of functional groups with similar features to these pharmacophoric points in the active site of Bcl-2 first. Then their most energetically favorable minima in the positions near the pharmacophoric points were used to optimize the distances between pharmacophoric points. By examining the binding modes of several inhibitors from the same structural type, it was found that the more potent the inhibitor was, the closer it was to the optimized distances between pharmacophoric points. The optimized 3D pharmacophore model obtained in this paper may provide a good starting point for further rational design of Bcl-2 inhibitors.

A broadband dual-polarization slotted waveguide planar antenna array for X-band SAR application is presented. The vertical polarization (VP) is realized with a ridged-waveguide longitudinal slot linear antenna array, while the horizontal polarization (HP) is realized with an untilted narrow-wall slot linear array. The basic part of the array is a subarray comprising an HP and a VP slotted waveguide single-polarization linear array. Each single-polarization linear array with 16 uniformly radiating elements is divided into four groups and fed by a 4-way waveguide power divider to broaden the bandwidth. The ridged slotted waveguide linear array for the VP is fed by a novel waveguide power divider which adapts to the compact width of the radiating guide. An untitled narrow-wall slot waveguide (half height) linear array excited by shaped irises for the HP, which can achieve very low cross polarization, is used. The benefits of this dual-polarization waveguide array, particularly when compared to microstrip arrays, are very low loss and good cross-polarization suppression. A panel of 16/spl times/16 dual-polarized waveguide slotted antenna array is designed, fabricated and measured. The measured results show a good agreement with the calculated ones, verifying the design. With the active transmit/receive module used, an active phased array antennas consisting of several hundreds of dual polarized subarrays can be carried out.

The three-dimensional structure of lanosterol 14alpha demethylase (P450(14DM), P450(51() of Candida albicans was modeled based on crystallographic coordinates of four prokaryotic cytochrome P450(S): P450BM3, P450cam, P450terp and P450eryF. The sequence of P450(51) was aligned to those of known proteins using a knowledge-based alignment method. The main chain coordinates of the structurally conserved regions (SCRs)) were transferred directly from the corresponding coordinates of P450BM3. The side chain conformations of SCRs) were determined based on the equivalent residues of four crystal structures which had the highest homologous scores. The model was then refined using molecular mechanics and molecular dynamics.The rationale of the resulting model were validated by Ramachandran plot,Profile-3K and hydropathy plot analysis. The structure-functionally important residues, such as the heme binding residues, the residues interacting with redox-partner protein and/or involved in electron transfer, the residues lining substrate access channel and the substrate binding residues, were identified from the model. These residues are candidates for further site-directed mutagensis and site-specific antipeptide antibody binding experiments.