14 results on '"Jia-Dong Sun"'
Search Results
2. Histone deacetylase inhibitors attenuated interleukin-1β-induced chondrogenesis inhibition in synovium-derived mesenchymal stem cells of the temporomandibular joint
- Author
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Wen-ting Liao, Jia-dong Sun, Yun Wang, Yi-qing He, Kai Su, Yun-yang Lu, Guiqing Liao, and Yang-peng Sun
- Subjects
histone deacetylase inhibitors ,temporomandibular joint ,synovium ,mesenchymal stem cell ,il-1β ,synovium-derived mesenchymal stem cells ,chondrogenesis ,interleukin-1β ,il-6 ,matrix metalloproteinase 13 ,cartilage ,mouse model ,arthritis ,condylar cartilage ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Aims: In the repair of condylar cartilage injury, synovium-derived mesenchymal stem cells (SMSCs) migrate to an injured site and differentiate into cartilage. This study aimed to confirm that histone deacetylase (HDAC) inhibitors, which alleviate arthritis, can improve chondrogenesis inhibited by IL-1β, and to explore its mechanism. Methods: SMSCs were isolated from synovium specimens of patients undergoing temporomandibular joint (TMJ) surgery. Chondrogenic differentiation potential of SMSCs was evaluated in vitro in the control, IL-1β stimulation, and IL-1β stimulation with HDAC inhibitors groups. The effect of HDAC inhibitors on the synovium and condylar cartilage in a rat TMJ arthritis model was evaluated. Results: Interleukin (IL)-1β inhibited the chondrogenic differentiation potential of SMSCs, while the HDAC inhibitors, suberoylanilide hydroxamic acid (SAHA) and panobinostat (LBH589), attenuated inhibition of IL-1β-induced SMSC chondrogenesis. Additionally, SAHA attenuated the destruction of condylar cartilage in rat TMJ arthritis model. IL-6 (p < 0.001) and matrix metalloproteinase 13 (MMP13) (p = 0.006) were significantly upregulated after IL-1β stimulation, while SAHA and LBH589 attenuated IL-6 and MMP13 expression, which was upregulated by IL-1β in vitro. Silencing of IL-6 significantly downregulated MMP13 expression and attenuated IL-1β-induced chondrogenesis inhibition of SMSCs. Conclusion: HDAC inhibitors SAHA and LBH589 attenuated chondrogenesis inhibition of SMSC induced by IL-1β in TMJ, and inhibition of IL-6/MMP13 pathway activation contributes to this biological progress. This study provides a theoretical basis for the application of HDAC inhibitors in the treatment of TMJ arthritis. Cite this article: Bone Joint Res 2022;11(1):40–48.
- Published
- 2022
- Full Text
- View/download PDF
3. Quorum sensing signals improve the power performance and chlortetracycline degradation efficiency of mixed-culture electroactive biofilms
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Xiao-Long Cheng, Qiang Xu, Jia-Dong Sun, Chun-Rui Li, Qian-Wen Yang, Biao Li, Xue-Ying Zhang, Jun Zhou, and Xiao-Yu Yong
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Chemistry ,Electrochemistry ,Electrochemical energy conversion ,Earth sciences ,Environmental science ,Energy resources ,Science - Abstract
Summary: Electroactive biofilms (EABs) play an important role in bioelectrochemical systems due to their abilities to generate electrons and perform extracellular electron transfer (EET). Here, we investigated the effects of quorum sensing (QS) signals on power output, chlortetracycline degradation, and structure of EABs in MFCs treating antibiotic wastewater. The voltage output of MFCs with C4-HSL and PQS increased by 21.57% and 13.73%, respectively, compared with that without QS signals. The chlortetracycline degradation efficiency in closed-circuit MFCs with C4-HSL and PQS increased by 56.53% and 50.04%, respectively, which resulted from the thicker biofilms, higher biomass, and stronger activities. Additionally, QS signals induced the heterogeneous distribution of EPS for a balance between self-protection and EET under environmental pressure. Geobacter prevailed by the addition of QS signals to resist high chlortetracycline concentration. Our results provided a broader understanding on regulating EABs within electrode interface to improve their performance for environmental remediation and clean energy development.
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- 2022
- Full Text
- View/download PDF
4. Cryopreservation of porcine skin-derived stem cells using melatonin or trehalose maintains their ability to self-renew and differentiate
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Jia-Dong Sun, Yu Sun, Tian Qiao, Shu-Er Zhang, Paul W. Dyce, Yuan-Wei Geng, Ping Wang, Wei Ge, Wei Shen, and Shun-Feng Cheng
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Cryopreservation ,Cryoprotective Agents ,Cell Survival ,Swine ,Stem Cells ,Animals ,Trehalose ,Dimethyl Sulfoxide ,General Medicine ,General Agricultural and Biological Sciences ,General Biochemistry, Genetics and Molecular Biology ,Melatonin - Abstract
Porcine skin-derived stem cells (pSDSCs) are a type of adult stem cells (ASCs) that retain the ability to self-renew and differentiate. Currently, pSDSCs research has entered an intense period of development; however there has been no research regarding methods of cryopreservation. In this paper, we explored an efficient cryopreservation method for pSDSCs. Our results demonstrated that cryopreserving 50 μm diameter pSDSCs aggregates resulted in a lower apoptosis rate and a greater ability to proliferate to form larger spherical cell aggregates than during single-cell cryopreservation. To further optimize the cryopreservation method, we added different concentrations of melatonin (N-acetyl-5-methoxytryptamine, MLT) and trehalose (d-trehalose anhydrous, TRE) to act as cryoprotectants (CPAs) for the pSDSCs. After comparative experiments, we found that the cryopreservation efficiency of 50 mM TRE was superior. Further experiments demonstrated that the reason why 50 mM TRE improved cryopreservation efficiency was that it reduced the intracellular oxidative stress and mitochondrial damage caused by cryopreservation. Taken together, our results suggest that cryopreserving 50 μm diameter pSDSCs aggregates in F12 medium with 10% dimethyl sulfoxide (DMSO) and 50 mM TRE promotes the long-term storage of pSDSCs.
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- 2022
5. Effects of Interleukin-1β on Long Noncoding RNA and mRNA Expression Profiles of Human Synovial Fluid-derived Mesenchymal Stem Cells
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Yang-peng Sun, Yun-yang Lu, Jianyu Chen, Jia-hao Bao, Hong Zhang, Jia-dong Sun, and Wen-ting Liao
- Abstract
Synovial fluid-derived mesenchymal stem cells (SFMSCs) play important regulatory roles in the physiological balance of the temporomandibular joint. Interleukin (IL)-1β regulates the biological behavior of SFMSCs; however, the effects of IL-1β on long noncoding RNA (lncRNA) and mRNA expression in SFMSCs in the temporomandibular joint are unclear. Here, we evaluated the lncRNA and mRNA expression profiles of IL-1β-stimulated SFMSCs. Using microarrays, we identified 286 lncRNAs (222 upregulated, 64 downregulated) and 304 mRNAs (242 upregulated, 62 downregulated) that were differentially expressed after treatment with IL-1β (fold change ≥ 2, P < 0.05). Kyoto Encyclopedia of Genes and Genomes pathway analysis found that one of the most significantly enriched pathways was the NF-κB pathway. Five paired antisense lncRNAs and mRNAs, eight paired enhancer lncRNAs and mRNAs, and nine paired long intergenic noncoding RNAs and mRNAs were predicted to be co-expressed. A network constructed by the top 30 k-score genes was visualized and evaluated. We found a co-expression relationship between ENST00000427824 and ENST00000307407 and between LOC541472 and IL6, which are related to NF-κB pathway activation. Overall, our results provide important insights into changes in lncRNA and mRNA expression in IL-1β-stimulated SFMSCs, which can facilitate the identification of potential therapeutic targets.
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- 2021
6. Quorum sensing signals improve the power performance and chlortetracycline degradation efficiency of mixed-culture electroactive biofilms
- Author
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Xiao-Long Cheng, Qiang Xu, Jia-Dong Sun, Chun-Rui Li, Qian-Wen Yang, Biao Li, Xue-Ying Zhang, Jun Zhou, and Xiao-Yu Yong
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Multidisciplinary - Abstract
Electroactive biofilms (EABs) play an important role in bioelectrochemical systems due to their abilities to generate electrons and perform extracellular electron transfer (EET). Here, we investigated the effects of quorum sensing (QS) signals on power output, chlortetracycline degradation, and structure of EABs in MFCs treating antibiotic wastewater. The voltage output of MFCs with C4-HSL and PQS increased by 21.57% and 13.73%, respectively, compared with that without QS signals. The chlortetracycline degradation efficiency in closed-circuit MFCs with C4-HSL and PQS increased by 56.53% and 50.04%, respectively, which resulted from the thicker biofilms, higher biomass, and stronger activities. Additionally, QS signals induced the heterogeneous distribution of EPS for a balance between self-protection and EET under environmental pressure. Geobacter prevailed by the addition of QS signals to resist high chlortetracycline concentration. Our results provided a broader understanding on regulating EABs within electrode interface to improve their performance for environmental remediation and clean energy development.
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- 2021
7. YAP regulates porcine skin-derived stem cells self-renewal partly by repressing Wnt/β-catenin signaling pathway
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Shu-Er Zhang, Yu Sun, Wei Shen, Shun-Feng Cheng, Ming-Yu Zhang, Paul W. Dyce, Jia-Dong Sun, Hong-Chen Yan, Massimo De Felici, and Francesca Gioia Klinger
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Small interfering RNA ,Histology ,Settore BIO/17 ,Chemistry ,Swine ,Stem Cells ,Transdifferentiation ,Wnt signaling pathway ,Cell Differentiation ,YAP-Signaling Proteins ,Cell Biology ,Cell biology ,Medical Laboratory Technology ,SOX2 ,Transcriptional regulation ,Animals ,Stem cell ,Signal transduction ,Molecular Biology ,Wnt Signaling Pathway ,beta Catenin ,Adult stem cell ,Cell Proliferation - Abstract
Background Skin-derived stem cells (SDSCs) are a class of adult stem cells (ASCs) that have the ability to self-renew and differentiate. The regulation mechanisms involved in the differentiation of ASCs is a hot topic. Porcine models have close similarities to humans and porcine SDSCs (pSDSCs) offer an ideal in vitro model to investigate human ASCs. To date, studies concerning the role of yes-associated protein (YAP) in ASCs are limited, and the mechanism of its influence on self-renewal and differentiation of ASCs remain unclear. In this paper, we explore the link between the transcriptional regulator YAP and the fate of pSDSCs. Results We found that YAP promotes the pluripotent state of pSDSCs by maintaining the high expression of the pluripotency genes Sox2, Oct4. The overexpression of YAP prevented the differentiation of pSDSCs and the depletion of YAP by small interfering RNA (siRNAs) suppressed the self-renewal of pSDSCs. In addition, we found that YAP regulates the fate of pSDSCs through a mechanism related to the Wnt/β-catenin signaling pathway. When an activator of the Wnt/β-catenin signaling pathway, CHIR99021, was added to pSDSCs overexpressing YAP the ability of pSDSCs to differentiate was partially restored. Conversely, when XAV939 an inhibitor of Wnt/β-catenin signaling pathway, was added to YAP knockdown pSDSCs a higher self-renewal ability resulted. Conclusions our results suggested that, YAP and the Wnt/β-catenin signaling pathway interact to regulate the fate of pSDSCs.
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- 2021
8. Production of poly-γ-glutamic acid (γ-PGA) from xylose-glucose mixtures by Bacillus amyloliquefaciens C1
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Ping Wei, Jun Zhou, Wang Yajun, An Wei, Chen Tang, Zhiying Yan, Jia-Dong Sun, and Xiaoyu Yong
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Bacillus amyloliquefaciens ,biology ,Chemistry ,Catabolite repression ,Lignocellulosic biomass ,Environmental Science (miscellaneous) ,Xylose ,biology.organism_classification ,Agricultural and Biological Sciences (miscellaneous) ,Hydrolysate ,Diauxic growth ,chemistry.chemical_compound ,Xylose metabolism ,Fermentation ,Food science ,Biotechnology - Abstract
Due to the promising applications, the demand to enhance poly-γ-glutamic acid (γ-PGA) production while decreasing the cost has increased in the past decade. Here, xylose/glucose mixture and corncob hydrolysate (CCH) was evaluated as alternatives for γ-PGA production by Bacillus amyloliquefaciens C1. Although both have been validated to support cell growth, glucose and xylose were not simutaneously consumed and exhibited a diauxic growth pattern due to carbon catabolite repression (CCR) in B. amyloliquefaciens C1, while the enhanced transcription of araE alleviated the xylose transport bottleneck across a cellular membrane. Additionally, the xyl operon (xylA and xylB), which was responsible for xylose metabolism, was strongly induced by xylose at the transcriptional level. When cultured in a mixed medium, xylR was sharply induced to 3.39-folds during the first 8-h while reduced to the base level similar to that in xylose medium. Finally, pre-treated CCH mainly contained a mixture of glucose and xylose was employed for γ-PGA fermentation, which obtained a final concentration of 6.56 ± 0.27 g/L. Although the glucose utilization rate (84.91 ± 1.81%) was lower than that with chemical substrates, the xylose utilization rate (43.41 ± 2.14%) and the sodium glutamate conversion rate (77.22%) of CCH were acceptable. Our study provided a promising approach for the green production of γ-PGA from lignocellulosic biomass and circumvent excessive non-food usage of glucose.
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- 2021
9. Production of poly-γ-glutamic acid (γ-PGA) from xylose-glucose mixtures by
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Jia-Dong, Sun, Chen, Tang, Jun, Zhou, Ping, Wei, Ya-Jun, Wang, Wei, An, Zhi-Ying, Yan, and Xiao-Yu, Yong
- Subjects
Original Article - Abstract
Due to the promising applications, the demand to enhance poly-γ-glutamic acid (γ-PGA) production while decreasing the cost has increased in the past decade. Here, xylose/glucose mixture and corncob hydrolysate (CCH) was evaluated as alternatives for γ-PGA production by Bacillus amyloliquefaciens C1. Although both have been validated to support cell growth, glucose and xylose were not simutaneously consumed and exhibited a diauxic growth pattern due to carbon catabolite repression (CCR) in B. amyloliquefaciens C1, while the enhanced transcription of araE alleviated the xylose transport bottleneck across a cellular membrane. Additionally, the xyl operon (xylA and xylB), which was responsible for xylose metabolism, was strongly induced by xylose at the transcriptional level. When cultured in a mixed medium, xylR was sharply induced to 3.39-folds during the first 8-h while reduced to the base level similar to that in xylose medium. Finally, pre-treated CCH mainly contained a mixture of glucose and xylose was employed for γ-PGA fermentation, which obtained a final concentration of 6.56 ± 0.27 g/L. Although the glucose utilization rate (84.91 ± 1.81%) was lower than that with chemical substrates, the xylose utilization rate (43.41 ± 2.14%) and the sodium glutamate conversion rate (77.22%) of CCH were acceptable. Our study provided a promising approach for the green production of γ-PGA from lignocellulosic biomass and circumvent excessive non-food usage of glucose.
- Published
- 2020
10. A novel core-shell Fe@Co nanoparticles uniformly modified graphite felt cathode (Fe@Co/GF) for efficient bio-electro-Fenton degradation of phenolic compounds
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Zhiying Yan, Biao Li, Chen Tang, Jun Zhou, Xiaoyu Yong, Jia-Dong Sun, Honghua Jia, and Xiayuan Wu
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Environmental Engineering ,010504 meteorology & atmospheric sciences ,Chemistry ,Decarboxylation ,Radical ,010501 environmental sciences ,Electrochemistry ,01 natural sciences ,Pollution ,Redox ,Catalysis ,chemistry.chemical_compound ,Vanillic acid ,Environmental Chemistry ,Reactivity (chemistry) ,Graphite ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Nuclear chemistry - Abstract
In this study, a core-shell Fe@Co nanoparticles uniformly modified graphite felt (Fe@Co/GF) was fabricated as the cathode by one-pot self-assembly strategy for the degradation of vanillic acid (VA), syringic acid (SA), and 4-hydroxybenzoic acid (HBA) in the Bio-Electro-Fenton (BEF) system. The Fe@Co/GF cathode showed dual advantages with excellent electrochemical performance and catalytic reactivity not only due to the high electron transfer efficiency but also the synergistic redox cycles between Fe and Co species, both of which significantly enhanced the in situ generation of H2O2 and hydroxyl radicals ( OH) to 152.40 μmol/L and 138.48 μmol/L, respectively. In this case, the degradation rates of VA, SA, and HBA reached 100, 94.32, and 100%, respectively, within 22 h. Representatively, VA was degraded and ultimately mineralized via demethylation, decarboxylation and ring-opening reactions. This work provided a promising approach for eliminating typical recalcitrant organic pollutants generated by the pre-treatment of lignocellulose resources.
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- 2021
11. Coordinated response of Au-NPs/rGO modified electroactive biofilms under phenolic compounds shock: Comprehensive analysis from architecture, composition, and activity
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Xiayuan Wu, Xiaoyu Yong, Chen Tang, Jun Zhou, Ping Wei, Honghua Jia, Jia-Dong Sun, Yifeng Zhang, and Biao Li
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Electroactive biofilms (EABs) ,Environmental Engineering ,0208 environmental biotechnology ,Oxide ,Metal Nanoparticles ,02 engineering and technology ,010501 environmental sciences ,Electrochemistry ,01 natural sciences ,Nanomaterials ,Catalysis ,law.invention ,chemistry.chemical_compound ,Electron transfer ,Extracellular polymeric substance ,law ,Phenolic compounds shock ,Electrodes ,Waste Management and Disposal ,Au-NPs/rGO ,0105 earth and related environmental sciences ,Water Science and Technology ,Civil and Structural Engineering ,Chemistry ,Graphene ,Ecological Modeling ,Biofilm ,Pollution ,Extracellular electron transfer (EET) ,020801 environmental engineering ,Chemical engineering ,Biofilms ,Extracellular polymeric substances (EPS) ,Graphite ,Gold ,Geobacter - Abstract
Electroactive biofilms (EABs) can be integrated with conductive nanomaterials to boost extracellular electron transfer (EET) for achieving efficient waste treatment and energy conversion in bioelectrochemical systems. However, the in situ nanomaterial-modified EABs of mixed-culture, and their response under environmental stress are rarely revealed. Here, two nanocatalyst-decorated EABs were established by self-assembled Au nanoparticles-reduced graphene oxide (Au-NPs/rGO) in mixed-biofilms with different maturities, then their multi-property were analyzed under long-term phenolic shock. Results showed that the power density of Au-NPs/rGO decorated EABs was significantly enhanced by 28.66–42.82% due to the intensified EET pathways inside biofilms. Meanwhile, the electrochemical and catalytic performance of EABs were controllably regulated by 0.3–3.0 g/L phenolic compounds, which, however, resulted in differential alterations in their architecture, composition, and viability. EABs originated with higher maturity displayed more compact structure, lower thickness (110 μm), higher biomass (8.67 mg/cm2) and viability (0.85–0.91), endowing it better antishock ability to phenolic compounds. Phenolic-shock also induced the heterogeneous distribution of extracellular polymeric substances in terms of both spatial and bonding degrees of the decorated EABs, which could be regarded as an active response to strike a balance between self-protection and EET under environmental pressure. Our findings provide a broader understanding of microbe-electrode interactions in the micro-ecology interface and improve their performance in the removal of complex contaminants for sustainable remediation and new-energy development.
- Published
- 2021
12. Synthesis of nano-MoS2/TiO2 composite and its catalytic degradation effect on methyl orange
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Yufu Xu, Xian Guo Hu, Kun Hong Hu, and Jia Dong Sun
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Anatase ,Materials science ,Scanning electron microscope ,Mechanical Engineering ,Composite number ,Analytical chemistry ,Catalysis ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Mechanics of Materials ,Methyl orange ,General Materials Science ,Fourier transform infrared spectroscopy ,Visible spectrum ,BET theory - Abstract
A nano-MoS2/TiO2 composite was synthesized in H2 atmosphere by calcining a MoS3/TiO2 precursor, which was obtained via a quick deposition of MoS3 on anatase nano-TiO2 under a strong acidic condition. The obtained nano-MoS2/TiO2 composite was characterized by X-ray diffraction spectroscopy, Brunauer–Emmett–Teller (BET) surface area, scanning electron microscopy, high-resolution transmission electron microscopy, energy-dispersive spectrometry, ultraviolet–visible spectroscopy, and Fourier transform infrared spectroscopy. The results show that the composite had a high BET surface area because of its small size and irregularly layered structure. MoS2 in the composite was composed of typical layered structures with thicknesses of 2–8 nm and lengths of 10–40 nm. The composite contained a wide and intensive absorption at 400–700 nm, which is in the visible light region, and presented a positive catalytic effect on removing methyl orange from the aqueous solution. The catalytic activity of the composite was influenced by the initial concentration of methyl orange, the amount of the catalyst, the pH value, and the degradation temperature. In addition, the composite catalyst could be regenerated and repeatedly used via filtration three times. The deactivating catalyst could be reactivated after catalytic reaction by heating at 450 °C for 30 min in H2.
- Published
- 2010
13. Influence of memantine hydrochloride on short-term cognition and EEG power spectrum in patients with mild to moderate Alzheimer’s disease
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Hui Zhou, Liuqing Huang, Shuqi Huang, Shu-Zhen Dong, Wei-Hua Wu, Zhong-Xin Zhao, Jia-Dong Sun, and Ying Zhao
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medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Medicine ,In patient ,Cognition ,General Medicine ,Disease ,Electroencephalography ,Audiology ,business ,Memantine Hydrochloride ,Term (time) - Published
- 2009
14. Synthesis of nano-MoS2/TiO2 composite and its catalytic degradation effect on methyl orange.
- Author
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Kun Hong Hu, Xian Guo Hu, Yu Fu Xu, and Jia Dong Sun
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COMPOSITE materials ,ELECTRON microscopy ,X-ray diffraction ,SCANNING electron microscopy ,TRANSMISSION electron microscopy ,HYDROGEN-ion concentration - Abstract
A nano-MoS
2 /TiO2 composite was synthesized in H2 atmosphere by calcining a MoS3 /TiO2 precursor, which was obtained via a quick deposition of MoS3 on anatase nano-TiO2 under a strong acidic condition. The obtained nano-MoS2 /TiO2 composite was characterized by X-ray diffraction spectroscopy, Brunauer–Emmett–Teller (BET) surface area, scanning electron microscopy, high-resolution transmission electron microscopy, energy-dispersive spectrometry, ultraviolet–visible spectroscopy, and Fourier transform infrared spectroscopy. The results show that the composite had a high BET surface area because of its small size and irregularly layered structure. MoS2 in the composite was composed of typical layered structures with thicknesses of 2–8 nm and lengths of 10–40 nm. The composite contained a wide and intensive absorption at 400–700 nm, which is in the visible light region, and presented a positive catalytic effect on removing methyl orange from the aqueous solution. The catalytic activity of the composite was influenced by the initial concentration of methyl orange, the amount of the catalyst, the pH value, and the degradation temperature. In addition, the composite catalyst could be regenerated and repeatedly used via filtration three times. The deactivating catalyst could be reactivated after catalytic reaction by heating at 450 °C for 30 min in H2 . [ABSTRACT FROM AUTHOR]- Published
- 2010
- Full Text
- View/download PDF
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