Search

Additional file 1: Fig. S1. Intramuscular injection of B16-MPs inhibited tumorigenesis of B16-F10 melanoma. Fig. S2. Intramuscular inoculation of MPs from CT26 colon carcinoma cells rather than those from H22 hepatocarcinoma cells led to prevention of CT26 tumor growth. Fig. S3. T-MPs as a tumor vaccine presented a good safety. Fig. S4. B lymphocytes and T lymphocytes in BALB/c mice don't endocytose T-MPs. Fig. S5. T-MPs are mainly endocytosed by monocytes and macrophages in C57BL/6 mice. Fig. S6. T-MPs treatment doesn't cause the proliferation of moDCs in the dLN. Fig. S7. DNAs and RNAs were isolated from T-MPs.

In the face of the arrival of artificial intelligence era, English classroom teaching is bound to change in organizational form, activity design, teacher-student relationship and technology integration. This paper combines SPOC and rain classroom, from knowledge infusion to ability improvement, individual teaching to team teaching, static resources to dynamic resources, artificial evaluation to intelligent evaluation, which brings new development opportunities for education. This paper uses the combination of Internet and artificial intelligence to analyze the teaching mixed teaching mode based on SPOC+and WeChat Rain classroom on the Internet. Based on the analysis results, we novelty propose the mixed English teaching mode based on such platforms to reveal the combination of Internet and artificial intelligence. On the second basis, we propose that future English teachers should continue to develop in the integration of teaching resources, integration of educational technology, design of in-depth learning activities, improvement of professional quality and self-cultivation of personality charm, and actively adapt to the era of artificial intelligence for College English classroom teaching.

Laser cladding surface modification technology is widely used to strengthen and repair the surface of axially symmetrical parts. Based on the Gauss heat source model and COMSOL Multiphysics finite element analysis software, the surface modification process characteristics of laser cladding on the rotating substrate were studied by numerical simulation. The transient temperature field distribution under different process parameters was solved. Laser cladding pool corresponding to numerical simulation parameters was photographed by infrared high-speed camera. The results show that the temperature distribution of the Gauss heat source is delayed due to the normal distribution of heat flow and the cumulative effect of heat. In addition, with the increase of the rotational speed of the substrate, a cyclic preheating temperature band will be formed on the surface. The shape of molten pool obtained by temperature field analysis is droplet shape, which is basically consistent with the actual morphology of laser cladding molten pool.

Autism spectrum disorders (ASDs) comprise a number of heterogeneous neurodevelopmental diseases. Recent studies suggest that the abnormal transmission of neural signaling pathways is associated with the pathogenesis of autism. The aim of this study was to identify a link between the Notch signaling pathway and the pathogenesis of autism. In this study, we demonstrated that prenatal exposure to valproic acid (VPA) resulted in autistic-like behaviors in offspring rats and that the expression of the Notch signaling pathway-related molecules Notch1, Jagged1, Notch intracellular domain (NICD) and Hes1 increased in the prefrontal cortex (PFC), hippocampus (HC) and cerebellum (CB) of VPA rats compared to those of controls. However, inhibiting the Notch pathway with (3,5-Difluorophenacetyl)-L-alanyl-S-phenylglycine-2-butyl Ester (Dapt) reduced the overexpression of Notch pathway-related molecules in offspring rats. Notably, Dapt improved autistic-like behaviors in a VPA-exposed rat model of autism. Furthermore, we investigated whether Dapt improved autistic-like behavior in a VPA rat model by regulating autophagy and affecting the morphology of dendritic spines. We found that the expression of the autophagy-related proteins Beclin 1, LC3B and phospho-p62 in the PFC, HC and CB of VPA model rats increased after Notch signal activation and was inhibited by Dapt compared to those of controls. Moreover, postsynaptic density-95 (PSD-95) protein expression also increased significantly compared to that of VPA model rats. The density of dendritic spines decreased in the PFC of VPA rats treated with Dapt compared to that of VPA model rats. Our present results suggest that VPA induces an abnormal activation of the Notch signaling pathway. The inhibition of excessive Notch signaling activation by Dapt can alleviate autistic-like behaviors in VPA rats. Our working model suggests that the Notch signaling pathway participates in the pathogenesis of autism by regulating autophagy and affecting dendritic spine growth. The results of this study may help to elucidate the mechanism underlying autism and provide a potential strategy for treating autism.

In order to solve the poor performance of location in RSSI localization Algorithm, caused by the link between anchor nodes and node to be positioning, this paper presents a positioning algorithm, which chooses anchor nodes based on Link quality Select strategy (LS) in a wireless sensor network. The method combines LS which plays a key role in localization with Weighted Centroid Localization Algorithm. The theoretical analysis and simulation results show that it raises the node ratio which can be located, and the accuracy of positioning can be well improved.

Clustering routing algorithm is an important and widely used method, which can prolong the lifecycle of WSN. Existing clustering algorithm due to the randomness of selecting the cluster head, it is inevitable that the agreement exists choosing cluster head unreasonable, node energy consumption unbalanced. To address the above issues, this paper presents an improved clustering routing algorithm LEACH-KED on the basis of the uniform clustering, and proceeds simulation analysis. The simulation results show that the improved algorithm is effective in extending the network life cycle.