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Abstract Mucopolysaccharidosis type II (MPS II) is a rare X-linked recessive inherited lysosomal storage disease. With pathogenic variants of the IDS gene, the activity of iduronate-2-sulfatase (IDS) is reduced or lost, causing the inability to degrade glycosaminoglycans (GAGs) in cells and influencing cell function, eventually resulting in multisystemic manifestations, such as a coarse face, dysostosis multiplex, recurrent respiratory tract infections, and hernias. Diagnosing MPS II requires a combination of clinical manifestations, imaging examinations, urinary GAGs screening, enzyme activity, and genetic testing. Currently, symptomatic treatment is the main therapeutic approach. Owing to economic and drug availability issues, only a minority of patients opt for enzyme replacement therapy or hematopoietic stem cell transplantation. The limited awareness of the disease, the lack of widespread detection technology, and uneven economic development contribute to the high rates of misdiagnosis and missed diagnosis in China.

Abstract Background Maternal uniparental disomy for chromosome 6 (upd(Cajaiba MM, Witchel S, Madan-Khetarpal S, Hoover J, Hoffner L, Macpherson T, et al. Prenatal diagnosis of trisomy 6 rescue resulting in paternal UPD6 with novel placental findings. Am J Med Genet Part A. 2011;155 A(8):1996–2002.)mat) has been previously reported to cause intrauterine growth restriction (IUGR), but the specific clinical phenotype has not been defined. Based on clinical data from two new cases and patients from the literature, specific phenotypes and mechanisms will be discussed further. Case presentation : In case 1, a maternal isodisomy mixed with a heterodisomy was found on chromosome 6, including a regional absence of heterozygosity between 6q23.3 and 6q27. In case 2, a homozygous SCUBE3 mutation and upd(Cajaiba MM, Witchel S, Madan-Khetarpal S, Hoover J, Hoffner L, Macpherson T, et al. Prenatal diagnosis of trisomy 6 rescue resulting in paternal UPD6 with novel placental findings. Am J Med Genet Part A. 2011;155 A(8):1996–2002.)mat, involving the 6p21.1-25.1 region were found. Clinical data related to upd(Cajaiba MM, Witchel S, Madan-Khetarpal S, Hoover J, Hoffner L, Macpherson T, et al. Prenatal diagnosis of trisomy 6 rescue resulting in paternal UPD6 with novel placental findings. Am J Med Genet Part A. 2011;155 A(8):1996–2002.)mat were also reviewed. Of all the 21 reported cases with upd(Cajaiba MM, Witchel S, Madan-Khetarpal S, Hoover J, Hoffner L, Macpherson T, et al. Prenatal diagnosis of trisomy 6 rescue resulting in paternal UPD6 with novel placental findings. Am J Med Genet Part A. 2011;155 A(8):1996–2002.)mat (including our 2 cases), 18 (85.7%) presented IUGR. Conclusions The phenotypes of the two newly identified patients with upd(Cajaiba MM, Witchel S, Madan-Khetarpal S, Hoover J, Hoffner L, Macpherson T, et al. Prenatal diagnosis of trisomy 6 rescue resulting in paternal UPD6 with novel placental findings. Am J Med Genet Part A. 2011;155 A(8):1996–2002.)mat further suggest that IUGR is associated with upd(Cajaiba MM, Witchel S, Madan-Khetarpal S, Hoover J, Hoffner L, Macpherson T, et al. Prenatal diagnosis of trisomy 6 rescue resulting in paternal UPD6 with novel placental findings. Am J Med Genet Part A. 2011;155 A(8):1996–2002.)mat and case 2 is the first reported upd(Cajaiba MM, Witchel S, Madan-Khetarpal S, Hoover J, Hoffner L, Macpherson T, et al. Prenatal diagnosis of trisomy 6 rescue resulting in paternal UPD6 with novel placental findings. Am J Med Genet Part A. 2011;155 A(8):1996–2002.)mat patient with a homozygous SCUBE3 gene mutation. However, the specific phenotypes involved in upd(Cajaiba MM, Witchel S, Madan-Khetarpal S, Hoover J, Hoffner L, Macpherson T, et al. Prenatal diagnosis of trisomy 6 rescue resulting in paternal UPD6 with novel placental findings. Am J Med Genet Part A. 2011;155 A(8):1996–2002.)mat and the related mechanisms need to be further studied.

Abstract Mucopolysaccharidoses (MPSs) are caused by a deficiency in the enzymes needed to degrade glycosaminoglycans (GAGs) in the lysosome. The storage of GAGs leads to the involvement of several systems and even to the death of the patient. In recent years, an increasing number of therapies have increased the treatment options available to patients. Early treatment is beneficial in improving the prognosis, but children with MPSs are often delayed in their diagnosis. Therefore, there is an urgent need to develop a method for early screening and diagnosis of the disease. Tandem mass spectrometry (MS/MS) is an analytical method that can detect multiple substrates or enzymes simultaneously. GAGs are reliable markers of MPSs. MS/MS can be used to screen children at an early stage of the disease, to improve prognosis by treating them before symptoms appear, to evaluate the effectiveness of treatment, and for metabolomic analysis or to find suitable biomarkers. In the future, MS/MS could be used to further identify suitable biomarkers for MPSs for early diagnosis and to detect efficacy.

Abstract Photocatalytic reduction of CO2 is a promising strategy to mitigate the effects of global warming by converting CO2 into valuable energy‐dense products. Silver bismuth iodide (SBI) is an attractive material owing to its tunable bandgap and favorable band‐edge positions for efficient CO2 photoreduction. In this study, SBI materials, including AgBi2I7, AgBiI4, Ag2BiI5, and Ag3BiI6 are first synthesized, through gas‐solid reaction by controlling the stoichiometric ratio of reactants. The X‐ray absorption near edge structure (XANES) and extended X‐ray absorption fine structure (EXAFS) results revealed that the distance between Ag‐I is proportional to the degree of Ag ions delocalization, which occupies the vacant sites. That greatly retards the charge recombination at vacant sites. In addition, the surface potential via photo‐assisted Kelvin probe force measurements of various SBI catalysts shows that Ag3BiI6 exhibits the highest surface potential change due to the rich delocalized Ag ions. This results in effective charge carrier transport and prevention of charge recombination at vacant sites. Taking the above advantages, the averaged CO and CH4 production rates for Ag3BiI6 achieved 0.23 and 0.10 µmol g−1 h−1, respectively. The findings suggest that Ag3BiI6 has a high potential as a novel photocatalyst for CO2 reduction and sheds light on the possibility of solving environmental contamination and sustainable energy crises.

The crystallography of transformation products with different cooling rates has been investigated in a typical low-carbon alloy steel, and its effect on low-temperature impact toughness uniformity in the thickness direction of heavy steel plates has been elucidated. The results show that both the full lath martenite (LM) microstructure transformed at the cooling rate of 45 K s−1 and granular bainite (GB) + lath bainite (LB) + LM microstructure transformed at the cooling rates of 3∼6 K s−1 have higher density of high-angle grain boundaries (HAGBs). The density of HAGBs in the full GB microstructure transformed at the cooling rate of 0.5 K s−1 is the lowest, and that in the LB + LM microstructure transformed at the cooling rates of 15∼22 K s−1 is the middle. In the GB + LB + LM microstructure, the division of austenite by multi-stage transformation reduces the LB and LM transformation temperatures, contributing to a weakening of variant selection and a tendency to close-packed plane grouping. Therefore, the GB + LB + LM microstructure achieves the refinement degree similar to the full LM microstructure, but slightly higher than that of the LB + LM microstructure. For the 60 mm thick heavy steel plate with ultra-fast cooling, the cooling rate decreases from 41 K s−1 at the subsurface to 14 K s−1 at the 1/4-thickness and then to 4 K s−1 at the 1/2-thickness. The full LM microstructure, LB + LM microstructure, and GB + LB + LM microstructure are obtained in sequence, resulting in a more uniform density of HAGBs. Consequently, the relatively uniform impact toughness at 143 K in the thickness direction is achieved with a small difference of ∼30 J.

Abstract Background This study aimed to assess the knowledge, attitudes, and practices (KAP) of the general population to community-acquired pneumonia (CAP) and COVID-19. Methods A cross-sectional study was conducted between September 2022 and February 2023, involving the general population from Ningbo Municipal Hospital of Traditional Chinese Medicine with a self-developed questionnaire. Results A total of 637 valid questionnaires were collected, with the majority of participants being female (62.48%). The mean score for knowledge, attitudes, and practices were 7.60 ± 2.39 (possible range: 0–12), 43.20 ± 4.57 (possible range: 11–55), and 34.57 ± 4.95 (possible range: 10–50), respectively. Multivariate logistic regression analysis indicated that master’s degree or above (OR = 6.04, 95% CI: 1.80-20.31, P = 0.004) and occupation in business or service careers (OR = 0.28, 95% CI: 0.17–0.48, P

Background: Serious secondary disasters caused by extreme natural weather conditions occur frequently, making it essential to establish a scientific and efficient modern emergency management system to maximize life-saving efforts. Methods: This study focuses on the uncertain environment of urban road networks and employs fuzzy theory to construct a 0–1 integer programming model for emergency evacuation paths that minimizes the average expected travel time. Results: We enhanced the neighborhood search strategy of the traditional ACO_time by incorporating the 2-opt and 3-opt perturbation mechanisms from the SA algorithm. Additionally, we utilized improved ant-volume and ant-perimeter models, along with their combinations, in the pheromone-updating mechanism of the basic ACO. The heuristic principles of the A* algorithm were integrated, introducing the joint influence of path and time into the heuristic function of the ACO algorithm. Conclusions: The IACO3 algorithm was tested on the Sioux Falls network and the Berlin Heisenheimer Center network. The computation time of the improved IACO3 algorithm was reduced by up to 20% compared to the original IACO3 algorithm in relation to the SA algorithm, with only a 4–5% increase in computation time compared to the ACO_time algorithm, which translates to an increase of merely 4–5 s. This demonstrates the superior solution efficiency of the IACO3 algorithm.

High-resolution imagery captured by drones can detect critical components on high-voltage transmission towers, providing inspection personnel with essential maintenance insights and improving the efficiency of power line inspections. The high-resolution imagery is particularly effective in enhancing the detection of fine details such as screws. The QYOLO algorithm, an enhancement of YOLOv8, incorporates context queries into the feature pyramid, effectively capturing long-range dependencies and improving the network’s ability to detect objects. To address the increased network depth and computational load introduced by query extraction, Ghost Separable Convolution (GSConv) is employed, reducing the computational expense by half and further improving the detection performance for small objects such as screws. The experimental validation using the Transmission Line Accessories Dataset (TLAD) developed for this project demonstrates that the proposed improvements increase the average precision (AP) for small objects by 5.5% and the F1-score by 3.5%. The method also enhances detection performance for overall targets, confirming its efficacy in practical applications.

Abstract Background Extravillous trophoblasts (EVTs) are essential cells during the formation of the placenta, with the major function of invading the maternal decidua, anchoring the developing placenta to the uterus, remodeling uterine arteries, and regulating immune responses to prevent rejection. During early pregnancy, the decidua undergoes a hypoxic and acidic microenvironment, which has been shown to participate in tumor cell migration, invasion, growth, and angiogenesis. Nevertheless, the mechanisms by which EVTs sense and respond to the acidic microenvironment, thereby executing their functions, remain poorly understood. Methods The effects of G protein-coupled receptor 65 (GPR65) on cell adhesion and other cellular functions were tested using JAR spheroids, mouse blastocysts, and HTR-8/SVneo cells. Specifically, we employed HTR-8/SVneo cells for gene overexpression and silencing to investigate the underlying mechanism of GPR65's impact on trophoblast cell function under acidic conditions. Additionally, villus tissue samples obtained from early pregnancy loss patients were utilized to explore the potential association between GPR65 and its related signaling pathway molecules with the disease. Results This study identified GPR65 expression widely in trophoblasts, with the highest level in EVTs. Importantly, optimal GPR65 levels are required for maintaining normal adhesion, migration, and invasion, whereas overexpression of GPR65 inhibits these functions by activating the cAMP-ERK signaling pathway, upregulating myosin light chain kinase (MYLK) and MYLK3 expression, and subsequently downregulating fibronectin. Consistently, elevated expression of GPR65, MYLK, and MYLK3 is observed in patients suffering from early pregnancy loss. Conclusions This work offers insights into the suppressive effects of GPR65 on EVT function under acidic conditions and highlights a putative target for therapeutic intervention in early pregnancy complications. Video Abstract

Two new triarylamine-based diamine monomers, namely, N,N′-bis(4-methoxyphenyl)-N,N′-bis(4-(4-aminophenyl-4′-methoxyphenylamino)phenyl)-p-phenylenediamine (3) and N,N′-bis(4-methoxyphenyl)-N,N′-bis(4-((4-aminophenyl-1-naphthyl)amino)phenyl)-p-phenylenediamine (7), were successfully synthesized and led to two series of electroactive polyamides by polycondensation reactions with common aromatic dicarboxylic acids. The polymers demonstrated multicolored electrochromism, high optical contrast, and remarkable enhancements in redox and electrochromic stability. Compared to other triarylamine-based polymers, the studied polyamides exhibited enhanced electrochromic stability (only 3~6% decay of its coloration efficiency at 445 nm after 14,000 switching cycles) at the first oxidation stage. The polyamides also showed strong absorption in the near-infrared region upon oxidation. Polymers with multicolored electrochromism and high redox stability can be developed by incorporation of four triarylamine cores in each repeat unit and electron-donating methoxy groups on the active sites of the triphenylamine units.

Abstract Circular RNAs (circRNAs) play a role in sepsis-related autophagy. However, the role of circRNAs in autophagy after sepsis-induced cardiomyopathy (SICM) is unknown, so we explored the circRNA expression profiles associated with autophagy in an acute sepsis mouse model. At a dose of 10 mg/kg, mice were intraperitoneally administered with lipopolysaccharides. The myocardial tissue was harvested after 6 h for microarray analysis, qRT-PCR, and western blotting. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes and Gene Set Enrichment Analysis were evaluated, and a competing endogenous RNA network was constructed, to evaluate the role of circRNAs related to autophagy in SICM. In total, 1,735 differently expressed circRNAs were identified in the LPS-treated group, including 990 upregulated and 745 downregulated circRNAs. The expression level of the autophagy-specific protein p62 decreased, while the ratio of LC3 II to LC3 I increased. Additionally, 309 mRNAs and 187 circRNAs were correlated with autophagy in myocardial tissue after SICM. Of these, 179 circRNAs were predicted to function as “miRNA sponges”. Some distinctive circRNAs and mRNAs found by ceRNA analysis might be involved in autophagy in SICM. These findings provide insights into circRNAs and identified new research targets that may be used to further explore the pathogenesis of SICM.

Abstract In this paper, we take the four-way shuttle system as the research object and establish the mathematical model of scheduling optimization based on the minimum time for the in/out operation optimization and path optimization scheduling problems of the four-way shuttle system. An improved genetic algorithm is used to solve the task planning, and an improved A* algorithm is used to solve the path optimization within the shelf level. The conflicts generated by the parallel operation of the four-way shuttle system are classified, and the improved A* algorithm based on the time window method is constructed for path optimization through the dynamic graph theory method to seek safe conflict-free paths. Through simulation example analysis, it is verified that the improved A* algorithm proposed in this paper has obvious optimization effect on the model of this paper.

Abstract In order to reveal the complex mechanism governing the mitotic/meiotic switch in female germ cells at epigenomic and genomic levels, we examined the chromatin accessibility (scATAC-seq) and the transcriptional dynamics (scRNA-seq) in germ cells of mouse embryonic ovary between E11.5 to 13.5 at single-cell resolution. Adopting a strict transcription factors (TFs) screening framework that makes it easier to understand the single-cell chromatin signature and a TF interaction algorithm that integrates the transcript levels, chromatin accessibility, and motif scores, we identified 14 TFs potentially regulating the mitotic/meiotic switch, including TCFL5, E2F1, E2F2, E2F6, E2F8, BATF3, SP1, FOS, FOXN3, VEZF1, GBX2, CEBPG, JUND, and TFDP1. Focusing on TCFL5, we constructed Tcfl5 +/− mice which showed significantly reduced fertility and found that decreasing TCFL5 expression in cultured E12.5 ovaries by RNAi impaired meiotic progression from leptotene to zygotene. Bioinformatics analysis of published results of the embryonic germ cell transcriptome and the finding that in these cells central meiotic genes (Stra8, Tcfl5, Sycp3, and E2f2) possess open chromatin status already at the mitotic stage together with other features of TCFL5 (potential capability to interact with core TFs and activate meiotic genes, its progressive activation after preleptotene, binding sites in the promoter region of E2f2 and Sycp3), indicated extensive amplification of transcriptional programs associated to mitotic/meiotic switch with an important contribution of TCFL5. We conclude that the identified TFs, are involved in various stages of the mitotic/meiotic switch in female germ cells, TCFL5 primarily in meiotic progression. Further investigation on these factors might give a significant contribution to unravel the molecular mechanisms of this fundamental process of oogenesis and provide clues about pathologies in women such as primary ovarian insufficiency (POI) due at least in part to meiotic defects.

Traumatic injuries in the central nervous system, such as traumatic brain injury and spinal cord injury, are associated with tissue inflammation and the infiltration of immune cells, which simultaneously affect the self-renewal and differentiation of neural stem cells. However, the tissue repair process instigated by endogenous neural stem cells is incapable of restoring central nervous system injuries without external intervention. Recently, resident/peripheral immune cells have been demonstrated to exert significant effects on neural stem cells. Thus, the restoration of traumatic injuries in the central nervous system by the immune intervention in neural stem cells represents a potential therapeutic method. In this review, we discuss the roles and possible mechanisms of immune cells on the self-renewal and differentiation of neural stem cells along with the prognosis of central nervous system injuries based on immune intervention. Finally, we discuss remaining research challenges that need to be considered in the future. Further elucidation of these challenges will facilitate the successful application of neural stem cells in central nervous system injuries.

Molecular docking plays an increasingly necessary role in interdisciplinary research, particularly in modern drug design. Pharmaceutical companies compose a trillion dollar per year industry and the public is generally unaware of how beneficial pharmaceutics come to be. Despite this increasing relevance in contemporary research, docking and, by extension, computational science, have been under-represented in undergraduate education in the chemical, biochemical, and biophysical sciences. We describe herein how a multidisciplinary approach is used to design novel inhibitors of the butyrylcholinesterase enzyme (BChE), an upregulated protein in patients suffering from Alzheimer’s disease. Students will then be able to compare their designed inhibitors to known BChE inhibitors via molecular docking using this easily adapted hands-on computational laboratory exercise or at-home activity that provides users with a module in which to learn the fundamentals of computer-aided drug design. While being well suited for upper-division courses in biology/biochemistry and physics/physical chemistry, the accessibility of this module allows for its incorporation into college curricula as early as second-term organic chemistry. Highly portable freeware makes this an ideal learning tool by which to infuse single- or multidisciplinary drug design reasoning into college level curricula at no cost to the student or instructor.

Abstract Background Fucosidosis is one of the rare autosomal recessive lysosomal storage diseases (LSDs) attributed to FUCA1 variants causing the deficiency of α-L-fucosidase in vivo. Α-L-fucosidase deficiency will cause excessive accumulation of fucosylated glycoproteins and glycolipids, which eventually leads to dysfunction in all tissue systems and presents with multiple symptoms. Fucosidosis is a rare disease which is approximately 120 cases have been reported worldwide (Wang, L. et al., J Int Med Res 48, 1-6, 2020). The number of reported cases in China is no more than 10 (Zhang, X. et al., J Int Med Res 49:3000605211005975, 2021). Case presentation The patient was an 8-year-old Chinese boy who presented with postnatal motor retardation, intellectual disability, short stature, language development retardation, coarse facial features, hepatomegaly, and diffuse angiokeratoma of both palms. His genetic testing showed the presence of a homozygous pathogenic variant (c.671delC) in the FUCA1 gene. In addition, the enzymatic activity of α-L-fucosidase was low. Ultimately, the patient was diagnosed with fucosidosis. Conclusions Fucosidosis is a rare lysosomal storage disease because of FUCA1 variants that cause the deficiency of α-L-fucosidase in vivo. An explicit diagnosis requires a combination of clinical manifestations, imaging examination, genetic testing and enzyme activity analysis. Early diagnosis plays an important role in fucosidosis.

Atractylodes macrocephala Koidz. (AM) is a rhizome plant traditionally used as an herbal medicine to normalize gastrointestinal function and treat inflammation-related diseases. However, there are no comprehensive studies that have examined the chemical composition or biological activity of the aerial parts of AM, which may be a promising raw material for the pharmaceutical industry. The aim of this study was to investigate the constituents, anti-oxidant, anti-inflammatory and anti-septic activities of the essential oil of the aerial parts of Atractylodes macrocephala (EOAPA). The chemical composition analysis of EOAPA by gas chromatography-quadrupole-time of flight-mass spectrometry (GC-Q/TOF-MS) led to the identification of 73 components, and the dominant compounds were hinesol (12.61%), β-eudesmol (11.51%), and atractylone (5.8%). EOAPA significantly inhibited lipopolysaccharide (LPS)-induced RAW264.7 cell expression of monocyte chemokine-1 (MCP-1), interleukin-6 (IL-6), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in a dose-dependent manner via regulating the nuclear transcription factor-κB (NF-κB) pathway, which may be related to its strong reactive oxygen species (ROS)-scavenging ability against LPS challenge at cellular level. While, its antioxidant activity in vitro was weak. Finally, LPS-induced septic shock in mice confirmed that EOAPA can effectively prevent mouse death by downregulating the levels of inflammatory factors in serum, such as IL-6, tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ). The above results showed that there is satisfactory potential for EOAPA to be developed and applied as a natural pharmaceutical and cosmetic additive.

ObjectiveTo explore the safety and diagnostic value of modified needle aspiration biopsy for superficial enlarged lymph nodes.MethodsA total of 88 patients with unknown diagnosis of superficial lymphadenopathy in our hospital (Wuxi Fifth People's Hospital) from January 2018 to July 2021 were recruited and then divided into two groups using the simple random grouping method. The study group included 48 patients who were punctured via modified needle aspiration biopsy, while the control group included 40 patients who were punctured via core needle biopsy (CNB) and had a better clinical evaluation. A BARD® automatic biopsy gun with a 18 G needle was used to puncture any enlarged lymph nodes, and specimens were obtained for pathological examination. The diagnostic positive rate of the two puncture methods was then compared and the complications involved in the two methods were observed.ResultsThe positive rate of definite pathological diagnosis was 79.2% (38/48) in the study group and 82.5% (33/40) in the control group. Both groups had similar diagnostic positive rates regarding tuberculosis and metastatic tumours in the lymph nodes (P > 0.05). Only slight bleeding was observed during the operations using the two puncture methods, with the bleeding rate of the study group 20.8% and that of the control group 15.0%, and the difference was not statistically significant (P > 0.05). No other complications were observed across both groups. Among the 48 patients with enlarged lymph nodes in the study group, there was no difference in the positive rate of diagnosis between enlarged lymph nodes larger than 20 mm and those smaller than 20 mm in the pathological, bacterial culture and cytology examinations (P > 0.05). The sensitivity, specificity, positive predictive values and negative prediction values of the improved lymph node lesions were 83.3%, 100%, 100% and 90.9%, respectively.ConclusionThe diagnostic efficacy and safety of modified needle aspiration biopsy in superficial swollen lymph nodes are equivalent to those of CNB, but the former is a more economical, simple and practical method for clinical settings and one that could be popularised in primary hospitals.

ObjectiveCigarettes have become the the biggest killer of contemporary female's health and beauty. What kind of health information is suitable for the general public is an important issue to be discussed globally. The purpose of this study is to generate systematic, rigorous, public-demand-oriented and appropriate core information relevant to tobacco control based on the best available evidence, combined with audience preferences and pre-dissemination content review from multidisciplinary expertise in order to improve the effectiveness of health communication of tobacco control.MethodsRelevant systematic reviews meta-analysis that reported smoking on risks of female disease were identified by searching PubMed, Embase, the Cochrane Library, Web of Science, Clinical Trials.gov, and the International Clinical Trial Registry Platform. The Grading of Recommendations Assessment, Development and Evaluation (GRADE) process was applied to assess the evidence in order to make rigorous core information. The audience prevalence survey was conducted to ensure that core information was targeted and tailored. Finally, the expert assessment was used for a pre-dissemination content review and to evaluate whether the core information was appropriate or not.ResultsThe final core information consisted of eight parts concerning the effects of smoking and female cardiovascular disease, diabetes, rheumatoid arthritis, respiratory disease, digestive system disease, mental disease, non-pregnant female reproductive system disease, as well as pregnant women and their fetuses. A total of 35 items of core information suitable for dissemination was included and the quality of evidence, the degree of public demand and the outcome of pre-dissemination content review were reported.ConclusionThe core information related to female cardiovascular system diseases, as well as liver cancer and upper gastrointestinal cancer is the preferred content for health communication of tobacco control. The quality of evidence for core information related to pregnant women and their infants, as well as diseases of reproductive system, respiratory system, and diabetes needs to be improved to meet high public demand. The core information related to mental disease is more suitable for dissemination to patients with mental illness than to the general public. Besides, dissemination of core information should be individualized. Evidence-based Core Information for Health Communication of Tobacco Control would be helpful to provide evidence support for health communication related to tobacco control and enhance public health literacy for international communities that have high smoking prevalence and related disease burden.

Human leukocyte antigen-G (HLA-G) is a non-classical human major histocompatibility complex (MHC-I) molecule with the membrane-bound and soluble types. HLA-G is primarily expressed by extravillous cytotrophoblast cells located at the maternal–fetal interface during pregnancy and is essential in establishing immune tolerance. This review provides a comprehensive understanding of the multiple molecular mechanisms by which HLA-G regulates the immune function of NK cells. It highlights that HLA-G binds to microRNA to suppress NK cell cytotoxicity and stimulate the secretion of growth factors to support fetal growth. The interactions between HLA-G and NK cells also activate senescence signaling, promoting spiral artery remodeling and maintaining the balance of maternal–fetal immune responses. In addition, HLA-G can inhibit the function of decidual T cells, dendritic cells, and macrophages. Overall, the interaction between trophoblast cells and immune cells mediated by HLA-G plays a crucial role in understanding immune regulation at the maternal–fetal interface and offers insights into potential treatments for pregnancy-related diseases.

In the context of sustainable development, this paper rationalises the outbound process of a four-way shuttle system with a focus on their modelling, performance evaluation and configuration using a parallel operation strategy to reduce resource waste, thus achieving sustainable development. The parallelism of the hoist and shuttle is innovatively incorporated into the four-way shuttle system, so the modelling content is divided into parallel and serial types. In the parallel operation strategy model, a separation–aggregation queueing network model is constructed, and the open-loop queueing network is innovatively solved using the maximum entropy method. In the serial operation strategy model, a semi-open-loop queuing network is constructed and solved using the geometric matrix method. By varying different parameters, the accuracy of the model is verified by Arena simulation with an error range of 10% or less, and the error of the system performance index calculation is reduced by 20% compared with the existing methods. Setting up 18 different sizes of shuttle systems provided a better performance than a single serial-operation strategy through the addition of parallel strategies, with an average reduction of 12.6% in the system response time and a minimum reduction of 1.8%. The conclusions of this paper were verified on the basis of an arithmetic case analysis.

To improve the threshold voltage and gate reliability of conventional enhancement-mode p-GaN-gated AlGaN/GaN high electron mobility transistors while maintaining a low on-resistance, an improved design solution for p-GaN HEMTs with P-I-N junction gate (PIN-HEMTs) has been proposed. Simulation results show that energy band modulation is achieved by adjusting the doping concentration and thickness of each layer of the PIN junction, and high-performance p-GaN gate HEMTs with adjustable threshold voltages ranging from 0.56 V to 4.75 V and gate breakdown voltages ranging from 19.8 V to 30.3 V would be prepared. The PIN-HEMT has a quasi-self-alignment property, which means that good gate control is independent of gate metal alignment. This not only improves the production efficiency but also solves the problems of weak gate control and electric field aggregation at the gate edge caused by the gate misalignment in conventional p-GaN gate HEMTs, thus realizing lower on-resistance and higher gate breakdown voltage, which demonstrates this proposed structure has excellent potentials for realizing effective and reliable high-power transistors. [ABSTRACT FROM AUTHOR]

Photocatalytic reduction of CO2 is a promising strategy to mitigate the effects of global warming by converting CO2 into valuable energy‐dense products. Silver bismuth iodide (SBI) is an attractive material owing to its tunable bandgap and favorable band‐edge positions for efficient CO2 photoreduction. In this study, SBI materials, including AgBi2I7, AgBiI4, Ag2BiI5, and Ag3BiI6 are first synthesized, through gas‐solid reaction by controlling the stoichiometric ratio of reactants. The X‐ray absorption near edge structure (XANES) and extended X‐ray absorption fine structure (EXAFS) results revealed that the distance between Ag‐I is proportional to the degree of Ag ions delocalization, which occupies the vacant sites. That greatly retards the charge recombination at vacant sites. In addition, the surface potential via photo‐assisted Kelvin probe force measurements of various SBI catalysts shows that Ag3BiI6 exhibits the highest surface potential change due to the rich delocalized Ag ions. This results in effective charge carrier transport and prevention of charge recombination at vacant sites. Taking the above advantages, the averaged CO and CH4 production rates for Ag3BiI6 achieved 0.23 and 0.10 µmol g−1 h−1, respectively. The findings suggest that Ag3BiI6 has a high potential as a novel photocatalyst for CO2 reduction and sheds light on the possibility of solving environmental contamination and sustainable energy crises. [ABSTRACT FROM AUTHOR]

Two new triarylamine-based diamine monomers, namely, N,N′-bis(4-methoxyphenyl)-N,N′-bis(4-(4-aminophenyl-4′-methoxyphenylamino)phenyl)-p-phenylenediamine (3) and N,N′-bis(4-methoxyphenyl)-N,N′-bis(4-((4-aminophenyl-1-naphthyl)amino)phenyl)-p-phenylenediamine (7), were successfully synthesized and led to two series of electroactive polyamides by polycondensation reactions with common aromatic dicarboxylic acids. The polymers demonstrated multicolored electrochromism, high optical contrast, and remarkable enhancements in redox and electrochromic stability. Compared to other triarylamine-based polymers, the studied polyamides exhibited enhanced electrochromic stability (only 3~6% decay of its coloration efficiency at 445 nm after 14,000 switching cycles) at the first oxidation stage. The polyamides also showed strong absorption in the near-infrared region upon oxidation. Polymers with multicolored electrochromism and high redox stability can be developed by incorporation of four triarylamine cores in each repeat unit and electron-donating methoxy groups on the active sites of the triphenylamine units. [ABSTRACT FROM AUTHOR]

ObjectiveThis study aimed to improve the cognition of mucolipidosis (ML) II and III alpha/beta by analyzing the clinical manifestations of two patients.MethodsThe clinical, biochemical, and molecular data of two clinical cases associated with ML II and III alpha/beta were analyzed and compared with other case reports of ML II and III alpha/beta.ResultsThe first patient was a 14-month-old girl who was hospitalized because of abnormal postnatal coarse facial features. The child had no abnormal birth history, but developed multiple abnormalities such as psychomotor retardation, abnormal facial features, bilateral limb muscle hypotonia, and genital abnormalities. The X-ray of the spine revealed multiple bone malformations. Brain magnetic resonance imaging (MRI) showed delayed myelination. Genetic testing showed the presence of two compound heterozygous pathogenic variants (c.1364C>T and c.1284+1G>T) in the GNPTAB gene. The second patient was an 18-month-old boy who was hospitalized for recurrent respiratory tract infections. The patient was a high-risk preterm infant with postnatal psychomotor retardation, language development retardation, intellectual disability, and coarse facial features. X-ray showed multiple bone malformations. Craniocerebral ultrasound showed bilateral ventricle widening. Genetic testing showed the presence of two compound heterozygous pathogenic variants (c.1284+1G>T and c.483delT) in the same gene.ConclusionsML II and III alpha/beta are rare autosomal-recessive lysosomal storage diseases that are attributed to GNPTAB variants that cause N-acetylglucosamine-1-phosphotransferase deficiency, finally leading to multiple clinical signs and symptoms. A proper ML II and/or III alpha/beta diagnosis requires a combined analysis of a patient's clinical manifestations, imaging examination, enzymatic analysis, and genetic testing results. Ultimately, genetic counseling is essential for this disease.

Recent years, Big Data, Cloud Computing and the advancement of the Internet of Things (IoT) played a major role in making smart city measures feasible. During this smart city, development, busy roadside activities and appropriate parking are considered as one of the major issues in the intelligent transportation system. Especially, in the city side region, the roadside activities are creating traffic misbehaviour problems which lead to various surveillance issues. So, in this study, the focus on the effective computer vision‐related roadside surveillance system is created to reduce the unwanted traffic and misbehaviour issues. Initially, road traffic images are collected with the help of the IoT device, which is processed by noise reduction techniques to eliminate the noise. After that, the vehicle object is identified in terms of geometric pattern matching algorithm as named as compound hierarchical‐deep models. Here, the geometric matching process is used to solve the uncertainty problems during the prediction of the vehicle in roadside activities. From the object detected data, roadside activities, such as vehicle position, occupancy, gap‐related decision, have been handled with the help of a fuzzy‐based decision‐making system. Furthermore, the efficiency of the system has been evaluated using respective case studies and experimental analysis.

With the development of e-commerce and trade, China's logistics transportation demand has increased significantly. To improve the operation efficiency of new energy trucks, logistics transportation companies need scientific management methods. They need to analyze a large number of real driving conditions for new energy trucks. Additionally, to reduce new energy trucks' energy consumption and pollutant emissions, automobile manufacturers have increased the research and development of new energy trucks, and the analysis of new energy truck driving conditions is the basis for the technical development and evaluation of new models. The research in this article is based on an actual project of an automobile manufacturing company, consulting a large amount of relevant domestic and foreign literature, summarizing the current status of driving conditions at home and abroad, explaining the principle of data collection for the driving conditions of new energy trucks, and developing in-vehicle data for driving conditions based on the principle transmission method and remote transmission method. Using the membership function and K-means clustering algorithm to determine the attribute characteristics of the new energy truck driving condition analysis, a truck driving condition analysis model is built, the software function of the model is designed, and a small amount of sample data is used to import the model instance to verify the model effectiveness. Finally, based on the constructed new energy truck driving condition analysis model, big data technology was used to perform a big data analysis experiment on the actual operation data of 200 trucks of an automobile manufacturing enterprise. The Spark big data calculation framework was used to perform stream calculation and offline analysis calculations on a large amount of data from the new energy trucks. The results show that the operating efficiency of the new energy truck driving condition analysis method using big data technology is significantly higher than that of traditional technology. This study provides a theoretical basis for controlling the energy consumption and pollutant emissions of new energy trucks in logistics transportation, and provides management and logistics support for transportation logistics companies. The technical development and evaluation of the company's new models provided data references.

Several studies have been conducted to improve the mechanical and other value-added properties of glass fiber-reinforced epoxy (GFRE) composites by the addition of different fillers. In this work, waste iron filings (WIFs) obtained from the steel industry were incorporated into GFRE composite samples in varying amounts of up to 50% (%w) to improve their mechanical and electrical properties. The results showed that, with increasing WIF loading from 0 w% to 50 w%, the resultant composite density gradually increased from 1.4 to 2.1 g/cm3. Surface hardness, Young’s modulus, and tensile strength also increased significantly with the addition of up to 9 w% of WIF followed by a significant drop with more WIF addition due to agglomeration. Overall, Young’s modulus of the GFRE samples with any WIF content was higher than that of the GFRE composite with no WIF. The elongation at break results showed that the GFRE samples were less ductile upon WIF addition, which decreased from 2% to 0.6% upon loading the composite with 50% WIF. In terms of electrical conductivity, the GFRE samples with WIF content of 15% or more were electrically conductive and their electrical conductivity increased with WIF content. It was clear that more WIF was needed to establish a percolated network in the GFRE composites to render them conductive. The electrical conductivity of the GFRE samples containing 15% WIFs was around 2.9 kS/m and increased to 35 kS/m upon the addition of 50% WIFs. These novel electrically conductive GFRE composites could be promising for structural dynamic monitoring systems in the construction industry. They also support the efforts for the utilization of waste materials towards a circular economy.

With the development of China’s express delivery industry, the number of express packaging has proliferated, leading to many problems such as environmental pollution and resource waste. In this paper, the process of reverse logistics network design for express packaging recycling is given as an example in the M region, and a four-level network containing primary recycling nodes, recycling centers, processing centers, and terminals is established. A candidate node selection model based on the K-means algorithm is constructed to cluster by distance from 535 courier outlets to select 15 candidate nodes of recycling centers and processing centers. A node selection model based on the NSGA-II algorithm is constructed to identify recycling centers and processing centers from 15 candidate nodes with minimizing total cost and carbon emission as the objective function, and a set of Pareto solution sets containing 43 solutions is obtained. According to the distribution of the solution set, the 43 solutions are classified into I, II, and III categories. The results indicate that the solutions corresponding to Class I and Class II solutions can be selected when the recycling system gives priority to cost, Class II and Class III solutions can be selected when the recycling system gives priority to environmental benefits, and Class III solutions can be selected when the society-wide recycling system has developed to a certain extent. In addition, this paper also randomly selects a sample solution from each of the three types of solution sets, conducts coding interpretation for site selection, vehicle selection, and treatment technology selection, and gives an example design scheme.