21 results on '"Ji HC"'
Search Results
2. High frequency plant regeneration from mature seedderived callus of Italian ryegrass (Lolium multiflorum) cultivars
- Author
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Lee, K-W, Choi, GJ, Kim, K-Y, Ji, HC, Park, HS, Yoon, SH, and Lee, S-H
- Subjects
Italian ryegrass, Lolium multiflorum, embryogenic callus, plant regeneration, growth additives - Abstract
In the present study, we have developed a high-frequency plant regeneration system for Italian ryegrass via callus culture using mature seeds as explants. Optimal embryogenic callus induction was found to occur in MS medium containing 5 mg l-1 2,4-D, 0.5 mg l-1 BA, 500 mg l-1 L-proline, 1 g l-1 casein hydrolysate, 30 g l-1 sucrose, 7 mg l-1 AgNO3, 2 mg l-1 CuSO4 and solidified with 3 g l-1 Gelrite. The highest regeneration rate was obtained in MS medium containing 1 mg l-1 2,4-D, 5 mg l-1 BA, 500 mg l-1 L-proline, 1 g l-1 casein hydrolysate, 1 mg l-1 thiamine-HCl, 30 g l-1 sucrose, 7 mg l-1 AgNO3, 2 mg l-1 CuSO4 and solidified with 3 g l-1 Gelrite. By using the most effective treatment determined for each parameter, the highest rates of embryogenic callus formation (48.9%) and regeneration (47.6%) were obtained with the Hwasan 101 cultivar. The overall plant regeneration rates of the examined cultivars ranged from 7.5 to 23.2%. Thus, optimization of regeneration frequency using mature seeds as explant material may offer a simple and efficient protocol for Italian ryegrass that may improve molecular breeding of this species.
- Published
- 2011
3. Identification of salt-stress induced differentially expressed genes in barley leaves using the annealingcontrol- primer-based GeneFishing technique
- Author
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Lee, S, Lee, K, Kim, K, Choi, GJ, Yoon, SH, Ji, HC, Seo, S, Lim, YC, and Ahsan, N
- Subjects
Barley, GeneFishing technique, salt stress - Abstract
In the present study, we have used an annealing-control-primer (ACP)-based differentially display RTPCR method to identify salt-stress-induced differentially expressed genes (DEGs) in barley leaves. Using 120 ACPs, a total of 11 up-regulated genes were identified and sequenced. Temporal expression patterns of some up-regulated DEGs in response to salt stress were further analyzed by Northern blot analysis. The possible roles of these identified genes are discussed within the context of their putative role in response to salt stress. Thus, the identification of some novel genes – such as SnRK1-type protein kinase; 17 kDa, class I, small heat shock protein; and RNase S-like protein precursor genes –may offer a new avenue for better understanding the salt stress response in plants, knowledge which might be helpful for developing future strategies.
- Published
- 2010
4. Significance and Possible Biological Mechanism for CLDN8 Downregulation in Kidney Renal Clear Cell Carcinoma Tissues.
- Author
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Ji HC, Li JD, Zhang GL, Huang ZG, Cheng JW, Li SH, Zhao CY, Tang YX, Qin K, Ma YL, Long Y, Chen G, and Qin B
- Abstract
Background: The clinical role of claudin 8 ( CLDN8 ) in kidney renal clear cell carcinoma (KIRC) remains unclarified. Herein, the expression level and potential molecular mechanisms of CLDN8 underlying KIRC were determined., Methods: High-throughput datasets of KIRC were collected from GEO, ArrayExpress, SRA, and TCGA databases to determine the mRNA expression level of the CLDN8 . In-house tissue microarrays and immunochemistry were performed to examine CLDN8 protein expression. A summary receiver operating characteristic curve (SROC) and standardized mean difference (SMD) forest plot were generated using Stata v16.0. Single-cell analysis was conducted to further prove the expression level of CLDN8 . A clustered regularly interspaced short palindromic repeats knockout screen analysis was executed to assess the growth impact of CLDN8 . Functional enrichment analysis was conducted using the Metascape database. Additionally, single-sample gene set enrichment analysis was implied to explore immune cell infiltration in KIRC., Results: A total of 17 mRNA datasets comprising 1,060 KIRC samples and 452 non-cancerous control samples were included in this study. Additionally, 105 KIRC and 16 non-KIRC tissues were analyzed using in-house immunohistochemistry. The combined SMD was -5.25 (95% confidence interval (CI): -6.13 to -4.37), and CLDN8 downregulation yielded an SROC area under the curve (AUC) close to 1.00 (95% CI: 0.99 - 1.00). CLDN8 downregulation was also confirmed at the single-cell level. Knocking out CLDN8 stimulated KIRC cell proliferation. Lower CLDN8 expression was correlated with worse overall survival of KIRC patients (hazard ratio of CLDN8 downregulation = 1.69, 95% CI: 1.2 - 2.4). Functional pathways associated with CLDN8 co-expressed genes were centered on carbon metabolism obstruction, with key hub genes ACADM , ACO2 , NDUFS1 , PDHB , SDHD , SUCLA2 , SUCLG1 , and SUCLG2., Conclusions: CLDN8 is downregulated in KIRC and is considered a potential tumor suppressor. CLDN8 deficiency may promote the initiation and progression of KIRC, potentially in conjunction with metabolic dysfunction., Competing Interests: The authors declare no conflict of interest., (Copyright 2024, Ji et al.)
- Published
- 2024
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5. Nitidine chloride regulates cell function of bladder cancer in vitro through downregulating Lymphocyte antigen 75.
- Author
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Wang SS, Zhai GQ, Huang ZG, Luo JY, He J, Huang JZ, Yang L, Xiao CN, Li SL, Chen KR, Chen YY, Ji HC, Ding JP, Li SH, Cheng JW, and Chen G
- Subjects
- Humans, Molecular Docking Simulation, Cell Line, Tumor, Cell Proliferation, Apoptosis, Lymphocytes, Cell Movement, Signal Transduction, Urinary Bladder Neoplasms drug therapy, Urinary Bladder Neoplasms genetics
- Abstract
Nitidine chloride (NC) is effective on cancer in many tumors, but its effect on bladder cancer (BC) is unknown. We conducted cell function experiments to verify the antineoplastic effect of NC on BC cell lines (5637, T24, and UM-UC-3) in vitro. Then, mRNAs of NC-treated and NC-untreated BC cells were extracted for mRNA sequencing. Differentially expressed genes (DEGs), expression analysis, and drug molecular docking were conducted to discover the target gene of NC. Finally, functional enrichment was analyzed to explore the underlying mechanisms. NC dramatically inhibited proliferation, migration, and invasion, and it induced apoptosis and arrested the S and G2/M phases of BC cell lines. Lymphocyte antigen 75 (LY75) appeared to be the target of NC. LY75 was highly expressed and had the ability to distinguish BC tissue from non-cancerous tissue. Then, drug molecular docking confirmed the targeting relationship between NC and LY75. Gene enrichment analysis showed that the downregulated genes, after being treated with NC, were mainly enriched in pathways relevant to cell pathophysiological processes. NC inhibits BC cell proliferation, migration, and invasion, induces apoptosis, and arrests cell cycles by downregulating the expression of LY75. This study provides molecular and theoretical bases for NC treatment of BC., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Published
- 2023
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6. Glutathione Restores Hg-Induced Morpho-Physiological Retardations by Inducing Phytochelatin and Oxidative Defense in Alfalfa.
- Author
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Rahman MA, Kabir AH, Mandal A, Roy SK, Song Y, Ji HC, and Lee KW
- Abstract
Mercury (Hg) is toxic to plants, but the effect of glutathione in Hg alleviation was never studied in alfalfa, an important forage crop. In this study, Hg toxicity showed morphological retardation, chlorophyll reduction, and PSII inefficiency, which was restored due to GSH supplementation in alfalfa plants treated with Hg. Results showed a significant increase of Hg, but Fe and S concentrations substantially decreased in root and shoot accompanied by the downregulation of Fe ( MsIRT1 ) and S ( MsSultr1;2 and MsSultr1;3 ) transporters in roots of Hg-toxic alfalfa. However, GSH caused a significant decrease of Hg in the shoot, while the root Hg level substantially increased, accompanied by the restoration of Fe and S status, relative to Hg-stressed alfalfa. The subcellular analysis showed a substantial deposition of Hg in the root cell wall accompanied by the increased GSH and PC and the upregulation of MsPCS1 and MsGSH1 genes in roots. It suggests the involvement of GSH in triggering PC accumulation, causing excess Hg bound to the cell wall of the root, thereby reducing Hg translocation in alfalfa. Bioinformatics analysis showed that the MsPCS1 protein demonstrated one common conserved motif linked to the phytochelatin synthase domain (CL0125) with MtPCS1 and AtMCS1 homologs. These in silico analysis further confirmed the detoxification role of MsPCS1 induced by GSH in Hg-toxic alfalfa. Additionally, GSH induces GSH and GR activity to counteract oxidative injuries provoked by Hg-induced H
2 O2 and lipid peroxidation. These findings may provide valuable knowledge to popularize GSH-derived fertilizer or to develop Hg-free alfalfa or other forage plants.- Published
- 2020
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7. Dioxin-like (DL-) polychlorinated biphenyls induced immunotoxicity through apoptosis in mice splenocytes via the AhR mediated mitochondria dependent signaling pathways.
- Author
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Du F, Zhao T, Ji HC, Luo YB, Wang F, Mao GH, Feng WW, Chen Y, Wu XY, and Yang LQ
- Subjects
- Animals, Body Weight drug effects, Cytokines genetics, Cytokines metabolism, Female, Male, Malondialdehyde metabolism, Mice, Mice, Inbred ICR, RNA, Messenger genetics, Reactive Oxygen Species metabolism, Receptors, Aryl Hydrocarbon genetics, Spleen cytology, Spleen enzymology, Superoxide Dismutase-1 metabolism, Thymus Gland drug effects, Thymus Gland enzymology, Thymus Gland metabolism, Apoptosis drug effects, Dioxins toxicity, Mitochondria metabolism, Polychlorinated Biphenyls toxicity, Receptors, Aryl Hydrocarbon metabolism, Signal Transduction, Spleen drug effects, Spleen immunology
- Abstract
Polychlorinated biphenyls (PCBs) would do serious damage to multiple systems, while coplanar polychlorinated biphenyls, the most toxic member of the family, has been widely taken into consideration. In this study, ICR mice were fed with different doses of PCB126 to explore the underlying molecular mechanisms on immunotoxicity. The results showed that PCB126 caused immunosuppression as evidenced by inhibiting the ratios of thymus and spleen weights, changing the organizational structure and decreasing levels and mRNA expression of TNF-α, IFN-γ and IL-2. PCB126 inhibited the SOD activity and spurred the accumulation of MDA in spleen and thymus. Meanwhile, it also disturbed the Nrf2 signaling pathway as evidenced by up-regulating the mRNA expression of Nrf2 and Keap1. Additionally, a remarkable reduction in the mRNA expression of AhR and enhancement in the mRNA expression of Cyp1 enzymes (Cyp1a1, Cyp1a2 and Cyp1b1) were observed, which increased the ROS levels. PCB126 could increase protein expression of Bax, Caspase-3, Caspase-8 and Caspase-9, while the protein expression of Bcl-2 was decreased. In summary, the results indicated that PCB126 modulated the AhR signaling pathway, which interacted with apoptosis and oxidative stress to induce immunotoxicity, enrich the immunotoxicological mechanisms of PCB126., (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Published
- 2019
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8. Design and Evaluation of a Novel Multiplex Real-Time PCR Melting Curve Assay for the Simultaneous Detection of Nine Sexually Transmitted Disease Pathogens in Genitourinary Secretions.
- Author
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Hu XM, Xu JX, Jiang LX, Deng LR, Gu ZM, Xie XY, Ji HC, Wang WH, Li LM, Tian CN, Song FL, Huang S, Zheng L, and Zhong TY
- Subjects
- Female, Humans, Male, Multiplex Polymerase Chain Reaction methods, Multiplex Polymerase Chain Reaction standards, Reproducibility of Results, Sensitivity and Specificity, Bodily Secretions, Real-Time Polymerase Chain Reaction methods, Real-Time Polymerase Chain Reaction standards, Sexually Transmitted Diseases diagnosis, Sexually Transmitted Diseases etiology, Urogenital System metabolism
- Abstract
Background: Sexually transmitted diseases (STD) are a major cause of infertility, long-term disability, ectopic pregnancy, and premature birth. Therefore, the development of fast and low-cost laboratory STD diagnostic screening methods will contribute to reducing STD-induced reproductive tract damage and improve women's health worldwide. In this study, we evaluated a novel multiplex real-time PCR melting curve assay method for the simultaneous detection of 9 STD pathogens, including Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum , and herpes simplex virus. Methods: The analytical performance of the method, including its limit of detection (LOD), specificity, repeatability, and effect on different DNA extraction kits were evaluated. Additionally, we obtained 1,328 clinical specimens from 3 hospitals to detect the 9 STD pathogens using multiplex real-time PCR melting curve and Sanger sequencing, to evaluate the sensitivity, specificity, and consistency of the assay method. Results: The results showed that the analytical sensitivity of the novel multiplex real-time PCR melting curve assay is very excellent, with LOD of DNA corresponding to <200 copies/μL for the DNA of the 9 STDs and 1.00 × 10
4 color change unit /ml for those of UU and UP. Additionally, this assay demonstrated excellent analytical specificity, excellent repeatability, and its results had no effect of different DNA extraction kits. The performance, in terms of sensitivity (91.06-100%) and specificity (99.14-100%), was remarkable, since the consistency between it and Sanger sequencing was more than 0.85 in the clinic. Conclusion: The novel multiplex real-time PCR melting curve assay method has high sensitivity and specificity, relatively low cost, and simple to use for the simultaneous detection of 9 STD pathogens in genitourinary secretions., (Copyright © 2019 Hu, Xu, Jiang, Deng, Gu, Xie, Ji, Wang, Li, Tian, Song, Huang, Zheng and Zhong.)- Published
- 2019
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9. Leveraging machine learning techniques for predicting pancreatic neuroendocrine tumor grades using biochemical and tumor markers.
- Author
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Zhou RQ, Ji HC, Liu Q, Zhu CY, and Liu R
- Abstract
Background: The incidence of pancreatic neuroendocrine tumors (PNETs) is now increasing rapidly. The tumor grade of PNETs significantly affects the treatment strategy and prognosis. However, there is still no effective way to non-invasively classify PNET grades. Machine learning (ML) algorithms have shown potential in improving the prediction accuracy using comprehensive data., Aim: To provide a ML approach to predict PNET tumor grade using clinical data., Methods: The clinical data of histologically confirmed PNET cases between 2012 and 2018 were collected. A method of minimum P for the Chi-square test was used to divide the continuous variables into binary variables. The continuous variables were transformed into binary variables according to the cutoff value, while the P value was minimum. Four classical supervised ML models, including logistic regression, support vector machine (SVM), linear discriminant analysis (LDA) and multi-layer perceptron (MLP) were trained by clinical data, and the models were labeled with the pathological tumor grade of each PNET patient. The performance of each model, including the weight of the different parameters, were evaluated., Results: In total, 91 PNET cases were included in this study, in which 32 were G1, 48 were G2 and 11 were G3. The results showed that there were significant differences among the clinical parameters of patients with different grades. Patients with higher grades tended to have higher values of total bilirubin, alpha fetoprotein, carcinoembryonic antigen, carbohydrate antigen 19-9 and carbohydrate antigen 72-4. Among the models we used, LDA performed best in predicting the PNET tumor grade. Meanwhile, MLP had the highest recall rate for G3 cases. All of the models stabilized when the sample size was over 70 percent of the total, except for SVM. Different parameters varied in affecting the outcomes of the models. Overall, alanine transaminase, total bilirubin, carcinoembryonic antigen, carbohydrate antigen 19-9 and carbohydrate antigen 72-4 affected the outcome greater than other parameters., Conclusion: ML could be a simple and effective method in non-invasively predicting PNET grades by using the routine data obtained from the results of biochemical and tumor markers., Competing Interests: Conflict-of-interest statement: None.
- Published
- 2019
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10. Importance of Mineral Nutrition for Mitigating Aluminum Toxicity in Plants on Acidic Soils: Current Status and Opportunities.
- Author
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Rahman MA, Lee SH, Ji HC, Kabir AH, Jones CS, and Lee KW
- Subjects
- Hydrogen-Ion Concentration, Plants metabolism, Trace Elements metabolism, Aluminum toxicity, Plants drug effects, Soil chemistry, Trace Elements pharmacology
- Abstract
Aluminum (Al) toxicity is one of the major limitations that inhibit plant growth and development in acidic soils. In acidic soils (pH < 5.0), phototoxic-aluminum (Al
3+ ) rapidly inhibits root growth, and subsequently affects water and nutrient uptake in plants. This review updates the existing knowledge concerning the role of mineral nutrition for alleviating Al toxicity in plants to acid soils. Here, we explored phosphorus (P) is more beneficial in plants under P-deficient, and Al toxic conditions. Exogenous P addition increased root respiration, plant growth, chlorophyll content, and dry matter yield. Calcium (Ca) amendment (liming) is effective for correcting soil acidity, and for alleviating Al toxicity. Magnesium (Mg) is able to prevent Al migration through the cytosolic plasma membrane in root tips. Sulfur (S) is recognized as a versatile element that alleviates several metals toxicity including Al. Moreover, silicon (Si), and other components such as industrial byproducts, hormones, organic acids, polyamines, biofertilizers, and biochars played promising roles for mitigating Al toxicity in plants. Furthermore, this review provides a comprehensive understanding of several new methods and low-cost effective strategies relevant to the exogenous application of mineral nutrition on Al toxicity mitigation. This information would be effective for further improvement of crop plants in acid soils.- Published
- 2018
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11. Metabolic profiling putatively identifies plasma biomarkers of male infertility using UPLC-ESI-IT-TOFMS.
- Author
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Zeng FJ, Ji HC, Zhang ZM, Luo JK, Lu HM, and Wang Y
- Abstract
Male infertility has become a global health problem. Currently, the diagnosis of male infertility depends on the results of semen quality or requires invasive surgical intervention. The process is complex and time-consuming. Metabolomics is an emerging platform with unique advantages in disease diagnosis and pathological mechanism research. In this study, ultra-performance liquid chromatography-electrospray ionization-ion trap-time of flight mass spectrometry (UPLC-ESI-IT-TOFMS) combined with chemometrics methods was used to discover potential biomarkers of male infertility based on non-targeted plasma metabolomics. Plasma samples from healthy controls (HC, n = 43) and various types of infertile patients, i.e. , patients having oligozoospermia (OS, n = 36), asthenospermia (AS, n = 56) and erectile dysfunction (ED, n = 45) were analyzed by UPLC-ESI-IT-TOFMS. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were performed. The results of OPLS-DA showed that HCs could be discriminated from infertile patients including OS ( R
2 = 0.903, Q2 = 0.617, AUC = 0.992), AS ( R2 = 0.985, Q2 = 0.658, AUC = 0.999) or ED ( R2 = 0.942, Q2 = 0.500, AUC = 0.998). Some potential biomarkers were successfully discovered by variable selection methods and variable important in the projection (VIP) in combination with the T -test. Statistical significance was set at p < 0.05; the Benjamini-Hochberg false discovery rate was used to reduce type 1 errors resulting from multiple comparisons. The identified biomarkers were associated with energy consumption, hormone regulation and antioxidant defenses in spermatogenesis. To elucidate the pathophysiology of male infertility, relative metabolic pathways were studied. It was found that male infertility is closely related to disturbed phospholipid metabolism, amino acid metabolism, steroid hormone biosynthesis metabolism, metabolism of fatty acids and products of carnitine acylation, and purine and pyrimidine metabolisms. Plasma metabolomics provides a novel strategy for the diagnosis of male infertility and offers a new insight to study pathogenesis mechanism., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)- Published
- 2018
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12. Combining Exosomes Derived from Immature DCs with Donor Antigen-Specific Treg Cells Induces Tolerance in a Rat Liver Allograft Model.
- Author
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Ma B, Yang JY, Song WJ, Ding R, Zhang ZC, Ji HC, Zhang X, Wang JL, Yang XS, Tao KS, Dou KF, and Li X
- Subjects
- Animals, Dendritic Cells drug effects, Interleukin-2 pharmacology, Models, Animal, Rats, T-Lymphocytes, Regulatory immunology, Tissue Donors, Transplantation Tolerance, Transplantation, Homologous, Dendritic Cells cytology, Exosomes metabolism, Liver Transplantation methods, T-Lymphocytes, Regulatory transplantation
- Abstract
Allograft tolerance is the ultimate goal in the field of transplantation immunology. Immature dendritic cells (imDCs) play an important role in establishing tolerance but have limitations, including potential for maturation, short lifespan in vivo and short storage times in vitro. However, exosomes (generally 30-100 nm) from imDCs (imDex) retain many source cell properties and may overcome these limitations. In previous reports, imDex prolonged the survival time of heart or intestine allografts. However, tolerance or long-term survival was not achieved unless immune suppressants were used. Regulatory T cells (Tregs) can protect allografts from immune rejection, and our previous study showed that the effects of imDex were significantly associated with Tregs. Therefore, we incorporated Tregs into the treatment protocol to further reduce or avoid suppressant use. We defined the optimal exosome dose as approximately 20 μg (per treatment before, during and after transplantation) in rat liver transplantation and the antigen-specific role of Tregs in protecting liver allografts. In the co-treatment group, recipients achieved long-term survival, and tolerance was induced. Moreover, imDex amplified Tregs, which required recipient DCs and were enhanced by IL-2. Fortunately, the expanded Tregs retained their regulatory ability and donor-specificity. Thus, imDex and donor-specific Tregs can collaboratively induce graft tolerance.
- Published
- 2016
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13. Enhancement effect of dihydroartemisinin on human γδ T cell proliferation and killing pancreatic cancer cells.
- Author
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Zhou ZH, Chen FX, Xu WR, Qian H, Sun LQ, Lü XT, Chen L, Zhang J, Ji HC, and Fei SJ
- Subjects
- Adolescent, Adult, Cell Line, Tumor, Cell Proliferation drug effects, Cell Survival drug effects, Granzymes metabolism, Humans, Interferon-gamma metabolism, Lysosomal-Associated Membrane Protein 1 metabolism, Pancreatic Neoplasms, Perforin metabolism, T-Lymphocyte Subsets metabolism, T-Lymphocytes metabolism, Young Adult, Adjuvants, Immunologic pharmacology, Antineoplastic Agents pharmacology, Artemisinins pharmacology, T-Lymphocyte Subsets drug effects, T-Lymphocytes drug effects
- Abstract
γδ T cells play important roles in innate immunity against tumors and infections. Inhibitory effect of dihydroartemisinin on growth of cancer cells has been found in recent years. In this study, we investigated the effect of dihydroartemisinin on human γδ T cell proliferation by MTT assay and killing activity against pancreatic cancer cells SW1990, BxPC-3 and PANC-1 by LDH release assay in vitro. Intracellular molecule alterations were verified by flow cytometry. The results suggested that appropriate concentration of dihydroartemisinin favored the expansion of γδ T cells and enhanced γδ T cell mediated killing activity against pancreatic cancer cells. Up-regulation of intracellular perforin, granzyme B expression and IFN-γ production may be the important mechanism of dihydroartemisinin on increased antitumor activity of γδ T cells., (© 2013. Published by Elsevier B.V. All rights reserved.)
- Published
- 2013
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14. [Effects of different stimulatory factors on functions of CIK cells].
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Liu JQ, Zhu Y, Chen FX, Zhou Y, Ji HC, Yang WY, Lyu XT, Zhang S, Tao ZZ, and Li Y
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- Cell Line, Tumor, Humans, Interferon-gamma metabolism, Interleukin-10 metabolism, Interleukin-12 metabolism, Interleukin-15 pharmacology, Interleukins pharmacology, Tumor Necrosis Factor-alpha metabolism, Cell Proliferation drug effects, Cytokine-Induced Killer Cells cytology, Cytokine-Induced Killer Cells drug effects
- Abstract
This study was aimed to investigate the effects of different stimulatory factors on proliferation and function of cytokine induced killer (CIK) cells. Peripheral blood mononuclear cells (PBMNC) were separated by Ficoll-Hypacue gradient. According to supplement of different stimulatory factors (CD28 mAb, IL-15 and IL-21), the experiment was divided into five groups:control group (CIK), CB28+IL-15+IL-21 group, IL-15+IL-21 group, CD28+IL-15 group and CD28+IL-21 group. Effects of different stimulatory factors on the proliferation of CIK cells were assayed by an automated hematology analyzer. Changes of granzyme B,perforin and CD107a were detected by flow cytometry. IL-10, IL-12, INF-γ and TNF-α were quantified by ELISA. Cytotoxicities on lung cancer cell line A549, breast adenocarcinoma cell line MFC-7 and human melanoma cell line HME1 were examined by lactate dehydrogenase release method. The results showed that there were significant differences among different groups. The highest proliferation index on days 10 was observed in group CD28mAb, IL-15 and IL-21(255.3 ± 6.3), which was higher than control group, IL-21+IL-15 group and CD28 mAb+IL-21 group (166.6 ± 13.5, 199.4 ± 15.0 and 228.8 ± 16.6) (P < 0.05). The expression of perforin in CD28 mAb+IL-15 group was higher than the other groups. The expression of perforin,GranB and CD107a of costimulatory groups was higher than control group. The cytotoxicities of CD28 mAb+IL-15 group on A549, MFC-7 and HME1 cells (82.2%, 59.3% and 70.6%) were much higher than that of control group (60.9%, 49.6% and 48.4%) (P < 0.05). The highest IFN-γsecretion was found in CD28 mAb, IL-15 and IL-21 groups. It is concluded that there are significant difference of proliferative capacity, cytokine secretion and cytotoxicity after being activated by different stimulatory factors. Adding corresponding stimulatory factors into the culture system displays a great value for target cells culture.
- Published
- 2013
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15. [LUTS in BPH patients with histological prostatitis before and after transurethral resection of the prostate].
- Author
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Huang XH, Qin B, Liang YW, Wu QG, Li CZ, Wei GS, Ji HC, Liang YB, Chen HQ, and Guan T
- Subjects
- Chronic Disease, Humans, Male, Prostatic Hyperplasia complications, Prostatitis complications, Transurethral Resection of Prostate, Treatment Outcome, Lower Urinary Tract Symptoms, Prostatic Hyperplasia surgery, Prostatitis surgery
- Abstract
Objective: To investigate the effects of transurethral resection of the prostate (TURP) on lower urinary tract symptoms (LUTS) in patients with benign prostatic hyperplasia (BPH) complicated by histological prostatitis., Methods: This study included 432 cases of BPH pathologically confirmed after TURP. Excluding those with LUTS-related factors before and after surgery and based on the international prostatitis histological classification of diagnostic criteria, the remaining 144 cases were divided into groups A (pure BPH, n = 30), B (mild inflammation, n = 55), C (moderate inflammation, n = 31), and D (severe inflammation, n = 28). Each group was evaluated for LUTS by IPSS before and a month after surgery., Results: A total of 399 cases (92.4%) were diagnosed as BPH with histological prostatitis, 269 (67.4%) mild, 86 (21.6%) moderate and 44 (11.0%) severe. The preoperative IPSS was 21.43 +/- 6.09 in group A, 21.75 +/- 5.97 in B, 27.84 +/- 4.18 in C and 31.00 +/- 2.92 in D, with statistically significant differences among different groups (P < 0.001) except between A and B (P = 1.000); the postoperative IPSS was 5.60 +/- 2.16 in A, 7.36 +/- 2.77 in B, 11.55 +/- 3.39 in C and 16.89 +/- 3.37 in D, with statistically significant differences among different groups (P < 0.01), and remarkably lower than the preoperative one (P < 0.001). Almost all the infiltrating inflammatory cells in BPH with histological prostatitis were lymphocytes., Conclusion: BPH is mostly complicated with histological chronic prostatitis. The severity of LUTS is higher in BPH patients with histological prostatitis than in those without before and after TURP, and positively correlated with the grade of inflammation. Those complicated with moderate or severe histological prostatitis should take medication for the management of LUTS.
- Published
- 2013
16. In vitro culture and characterization of alveolar bone osteoblasts isolated from type 2 diabetics.
- Author
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Sun DC, Li DH, Ji HC, Rao GZ, Liang LH, Ma AJ, Xie C, Zou GK, and Song YL
- Subjects
- Alkaline Phosphatase analysis, Collagen Type I analysis, Humans, Male, Middle Aged, Osseointegration physiology, Osteoblasts cytology, Osteoblasts pathology, Primary Cell Culture methods, Alveolar Process cytology, Calcification, Physiologic physiology, Dental Implants, Diabetes Mellitus, Type 2 physiopathology, Osteoblasts physiology, Osteocalcin analysis
- Abstract
In order to understand the mechanisms of poor osseointegration following dental implants in type 2 diabetics, it is important to study the biological properties of alveolar bone osteoblasts isolated from these patients. We collected alveolar bone chips under aseptic conditions and cultured them in vitro using the tissue explants adherent method. The biological properties of these cells were characterized using the following methods: alkaline phosphatase (ALP) chemical staining for cell viability, Alizarin red staining for osteogenic characteristics, MTT test for cell proliferation, enzyme dynamics for ALP contents, radio-immunoassay for bone gla protein (BGP) concentration, and ELISA for the concentration of type I collagen (COL-I) in the supernatant. Furthermore, we detected the adhesion ability of two types of cells from titanium slices using non-specific immunofluorescence staining and cell count. The two cell forms showed no significant difference in morphology under the same culture conditions. However, the alveolar bone osteoblasts received from type 2 diabetic patients had slower growth, lower cell activity and calcium nodule formation than the normal ones. The concentration of ALP, BGP and COL-I was lower in the supernatant of alveolar bone osteoblasts received from type 2 diabetic patients than in that received from normal subjects (P < 0.05). The alveolar bone osteoblasts obtained from type 2 diabetic patients can be successfully cultured in vitro with the same morphology and biological characteristics as those from normal patients, but with slower growth and lower concentration of specific secretion and lower combining ability with titanium than normal ones.
- Published
- 2012
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17. Effect of PDL-induced coherent crosstalk on polarization-division-multiplexed direct-detection systems.
- Author
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Ji HC, Lee JH, Kim H, Park PK, and Chung YC
- Abstract
We analyze the effect of polarization-dependent loss (PDL) on the performance of the polarization-division-multiplexed (PDM) signals in direct-detection systems. We present the theoretical analysis of the sensitivity penalty caused by PDL-induced coherent crosstalk and compare the results with experimental data obtained by using a simple variable PDL emulator based on a LiNbO3 Mach-Zehnder modulator. Also presented is the performance degradation caused by the polarization misalignment between the received signals and the polarization beam splitter at the receiver in the presence of PDL.
- Published
- 2009
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18. Inter-channel nonlinear crosstalk in analog phase-modulated wavelength-division-multiplexed systems.
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Kim H, Lee JH, and Ji HC
- Subjects
- Computer Simulation, Equipment Design, Equipment Failure Analysis, Light, Nonlinear Dynamics, Scattering, Radiation, Artifacts, Computer-Aided Design, Models, Theoretical, Optical Devices, Optical Fibers, Telecommunications instrumentation
- Abstract
We investigate the inter-channel nonlinear optical crosstalk in analog phase-modulated (PM) wavelength-division-multiplexed systems. The optical transmitters of the PM system produce constant optical intensity at the output. However, the chromatic dispersion of fiber induces amplitude fluctuations which in turn give rise to fiber nonlinearities to other channels through stimulated Raman scattering (SRS) and optical Kerr effect. We present theoretical analysis on SRS and cross-phase modulation (XPM) for PM systems and compare it with two-channel experiment and numerical simulation. The results show that PM systems significantly suppress SRS and XPM, compared to intensity-modulated systems. Our experiment and simulation results confirm that the total nonlinear crosstalk in the analog PM WDM system is reduced up to 15 dB., ((c) 2008 Optical Society of America)
- Published
- 2008
- Full Text
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19. Cost-effective colorless WDM-PON delivering up/down-stream data and broadcast services on a single wavelength using mutually injected Fabry-Perot laser diodes.
- Author
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Ji HC, Yamashita I, and Kitayama K
- Subjects
- Equipment Design, Equipment Failure Analysis, Japan, Communication, Information Storage and Retrieval methods, Lasers, Semiconductor, Semiconductors, Signal Processing, Computer-Assisted instrumentation
- Abstract
We propose and demonstrate for the first time a cost-effective and colorless wavelength-division-multiplexed passive optical network which can provide a full-duplex 155-Mb/s up/downstream data and broadcast signals on a single wavelength using light-injected optical sources and bidirectional transmission in a single fiber. The optical sources for downstream and upstream baseband data are Fabry-Perot laser diode (FPLD) at the central office and a reflective semiconductor optical amplifier (RSOA) at each optical network unit, respectively. The broadband light source (BLS) for the downstream is implemented by using mutually injected FPLDs. The transmission experiment shows that the error-free operation for baseband data and a high-quality broadcast signal with 3-dB CNR margin can be obtained.
- Published
- 2008
- Full Text
- View/download PDF
20. Physiological effects of RF exposure on hypersensitive people by a cell phone.
- Author
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Kim DW, Lee JH, Ji HC, Kim SC, Nam KC, and Cha EJ
- Subjects
- Adult, Blood Pressure radiation effects, Case-Control Studies, Female, Heart Rate radiation effects, Humans, Male, Respiration radiation effects, Young Adult, Cell Phone, Electromagnetic Fields adverse effects, Radio Waves adverse effects
- Abstract
Persons with electromagnetic hypersensitivity (EHS) complain of subjective symptoms such as headaches, insomnia, memory loss etc. resulting from radio frequency (RF) radiation by cellular phones. There have been various EHS provocation studies on heart rate, blood pressure, and subjective symptoms using GSM phones. However, there are few provocation studies on case-control study investigating simultaneously physiological parameters from CDMA phones. In this study, two volunteer groups of 18 self-declared EHS and 19 controls were exposed to both sham and real RF exposures by a CDMA cellular phone for half an hour each. We investigated the physiological parameters such as heart rates, respiration rates, and hear rate variability (HRV). In conclusion, the RF exposure by a CDMA cellular phone did not have any effects on the physiological parameters for both groups.
- Published
- 2008
- Full Text
- View/download PDF
21. [Long term observation in effects of potassium and calcium supplementation on arterial blood pressure and sodium metabolism in adolescents with higher blood pressure].
- Author
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Mu JJ, Liu ZQ, Yang J, Liang YM, Zhy DJ, Wang YX, Gao BL, Zhang XL, Ji HC, and Xu XL
- Subjects
- Adolescent, Adult, Blood Pressure Monitors, Female, Humans, Hypertension diet therapy, Male, Natriuresis, Single-Blind Method, Sodium, Dietary administration & dosage, Calcium, Dietary administration & dosage, Hypertension prevention & control, Potassium, Dietary administration & dosage, Sodium metabolism
- Abstract
Objective: To investigate the effects of potassium and calcium supplementation in table salt on reduction of arterial blood pressure and sodium metabolism in adolescents with higher blood pressure., Methods: A single blind placebo-controlled trial was carried out for two years in 220 adolescents with higher blood pressure, aged 18 - 22 years, who were randomly divided into supplementary group (n = 110) and control group (n = 110). Each of the subjects in the supplementary group and their family members was given 10 mmol of potassium and 10 mmol of calcium mixed in their table salt daily for 24 months., Results: Night urinary sodium and potassium excretion increased (urinary Na(+), P < 0.05; urinary K(+), P < 0.01) and blood pressure lowered by 5.3 mm Hg/1.8 mm Hg in average from the baseline in the supplementary group two years after potassium and calcium supplementation, as compared with that in the control group increased by (1.3/1.7) mm Hg., Conclusions: Adequate supplement of potassium and calcium in daily table salt intake was an effective way to prevent form hypertension and could promote their urinary sodium excretion and reduction of arterial blood pressure in adolescents with higher blood pressure.
- Published
- 2003
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