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Your search keyword '"Ji, You-Ren"' showing total 13 results
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13 results on '"Ji, You-Ren"'

1. Click chemistry-mediated enrichment of circulating tumor cells and tumor-derived extracellular vesicles for dual liquid biopsy in differentiated thyroid cancer

Author

Feng, Bing, Wang, Jing, Zhang, Ryan Y, Wei, Anna Yaxuan, Zhao, Chen, Yen, Ying-Tzu, Ji, You-Ren, Kim, Hyoyong, Ju, Yong, Smalley, Matthew, Zuo, Vivian Xufei, Cheng, Liwen, Phung, Aaron, Zhou, Ziang, Yu, Sitong, DiBernardo, Gabriella, Memarzadeh, Sanaz, Posadas, Edwin M, Chai-Ho, Wanxing, Agopian, Vatche, Lee, Junseok, Yeh, Michael W, Wu, James, Zheng, Guangjuan, Tseng, Hsian-Rong, and Zhu, Yazhen

Subjects
Biomedical and Clinical Sciences, Oncology and Carcinogenesis, Cancer, Clinical Research, Good Health and Well Being, Biomedical Engineering, Nanotechnology, Nanoscience & Nanotechnology, Medical biotechnology, Biomedical engineering
Abstract

Circulating tumor cells (CTCs) and tumor-derived extracellular vesicles (tEVs) are two crucial methodologies of liquid biopsy. Given their distinct size differences and release dynamics, CTCs and tEVs potentially offer synergistic capabilities in the non-invasive detection of differentiated thyroid cancer (DTC), a typically indolent tumor. We present the Combined DTC CTC/tEV Assay, integrating dual liquid biopsy processes: i) DTC CTC enrichment by Click Chips, followed by analysis of seven DTC-specific genes, and ii) DTC tEV enrichment by Click Beads, succeeded by mRNA cargo quantification in DTC tEVs. This method utilizes click chemistry, leveraging a pair of biorthogonal and highly reactive functional motifs (tetrazine, Tz, and trans-cyclooctene, TCO), to overcome the challenges encountered in the conventional immunoaffinity-based enrichment of CTCs and tEVs. The Combined DTC CTC/tEV Assay synergistically combines the diagnostic precision of CTCs with the sensitivity of tEVs, demonstrating superior diagnostic accuracy in DTC detection and boasting an AUROC of 0.99. This outperforms the individual diagnostic performance of using either DTC CTC or DTC tEV alone. This integration enables full utilization of a patient's blood sample, and marks a significant evolution in the development of nanomaterial-based liquid biopsy technologies to address challenging unmet clinical needs in cancer care.

Published
2024

13. Poly(allylguanidine)-Coated Surfaces Regulate TGF-β in Glioblastoma Cells to Induce Apoptosis viaNF-κB Pathway Activation

Author

Ji, You-Ren, Cheng, Ching-Chia, Lee, An-Li, Shieh, Jonathan Chang-Cheng, Wu, Hsin-Ju, Huang, Abel Po-Hao, Hsu, Yi-Hua, and Young, Tai-Horng

Abstract

Polycationic biomaterials are currently widely applied in neuronal cell cultures to promote cell adhesion and viability. However, polycations generally have cytotoxic properties that limit their application in the field of biomaterials. In this study, we examined the use of a novel polycation poly(allylguanidine) (PAG), which contains a guanidine group in the side chain and a structure similar to poly(allylamine hydrochloride) (PAH), an example of another commonly used polycation. Our findings showed that exposure to PAG induced apoptosis in glioblastoma (GBM) cells, while exposure to PAH induced necrosis. Compared to control groups, the PAG coating significantly limited the proliferation of GBM8901 in vitro and in vivo. Furthermore, GBM8901 cells exposed to the PAG coating exhibited increased levels of phospho-p65 and phosphor-IκB, implying that GBM8901 cells underwent apoptotic cell death viathe NF-κB pathway by the regulation of TGF-β. This result was further confirmed to be consistent with the experimental results from western blot protein analysis and apoptosis/necrosis assays. These findings indicate that the polycation PAG has the potential to not only suppress the proliferation of GBM8901 cancer cells but also improve the neural viability and promote the differentiation of neural stem/precursor cells into mature neurons. In conclusion, biomaterials such as PAG act as extremely potent options for applications in the treatment of pathological conditions such as brain cancer.

Published
2021
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