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8 results on '"Ji, Sheng-Cheng"'

1. Adaptive operation strategy for voltage stability enhancement in active DMFCs

Author

Yang, Qin-Wen, Hu, Xu-Qu, Lei, Xiu-Cheng, Zhu, Ying, Wang, Xing-Yi, and Ji, Sheng-Cheng

Subjects
Physics - Applied Physics, Physics - Fluid Dynamics
Abstract

An adaptive operation strategy for on-demand control of active direct methanol fuel cells (DMFCs) is proposed as an alternative method to enhance the voltage stability. A simplified semi-empirical model is firstly developed to describe I-V relationships based on uniform-designed experiments. It is then embedded into multi-objective optimizations to construct the adaptive operation strategy. Experimental studies are implemented on different DMFC systems to validate the proposed semi-empirical model, control strategy and system response to operational adjustments. Numerical simulations are also performed to investigate the underlying mechanisms of the proposed adaptive operation strategy. The results show that the adaptive operation strategy provides possibilities for voltage stability enhancement without the sacrifice of energy conversion efficiency. The adaptive operations are also found to be able to extend the range of operating current density or to decrease the voltage deviation according to one's requirements. Moreover, the response of DMFCs to operational adjustments is quick, which further validates the effectiveness and feasibility of the adaptive operation strategy in practical applications. The proposed strategy contributes to a guideline for the better control of output voltage from operating DMFC systems., Comment: 36 pages, 10 figures

Published
2018
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7. Characterization of novel M-superfamily conotoxins with new disulfide linkage

Author

Yu-Hong Han, Qiuyun Dai, Xiao-Xia Shao, Hui Jiang, Qi Wang, Duo-Duo Yuan, Li Liu, Cai Xiao, Cheng-Wu Chi, and Ji-Sheng Cheng

Subjects
Protein Folding, DNA, Complementary, Conus textile, Disulfide Linkage, Molecular Sequence Data, Venom, Biochemistry, Conus leopardus, Conus quercinus, Animals, Protein Isoforms, Amino Acid Sequence, Cysteine, Disulfides, Conotoxin, Cloning, Molecular, Molecular Biology, Conus marmoreus, Base Sequence, biology, Chemistry, Conus Snail, Cell Biology, biology.organism_classification, Conotoxins
Abstract

The M-superfamily with the typical Cys framework (-CC-C-C-CC-) is one of the seven major superfamilies of conotoxins found in the venom of cone snails. Based on the number of residues in the last Cys loop (between C4 and C5), M-superfamily conotoxins can be provisionally categorized into four branches (M-1, M-2, M-3, M-4) [Corpuz GP, Jacobsen RB, Jimenez EC, Watkins M, Walker C, Colledge C, Garrett JE, McDougal O, Li W, Gray WR, et al. (2005) Biochemistry44, 8176-8186]. Here we report the purification of seven M-superfamily conotoxins from Conus marmoreus (five are novel and two are known as mr3a and mr3b) and one known M-1 toxin tx3a from Conus textile. In addition, six novel cDNA sequences of M-superfamily conotoxins have been identified from C. marmoreus, Conus leopardus and Conus quercinus. Most of the above novel conotoxins belong to M-1 and M-2 and only one to M-3. The disulfide analyses of two M-1 conotoxins, mr3e and tx3a, revealed that they possess a new disulfide bond arrangement (C1-C5, C2-C4, C3-C6) which is different from those of the M-4 branch (C1-C4, C2-C5, C3-C6) and M-2 branch (C1-C6, C2-C4, C3-C5). This newly characterized disulfide connectivity was confirmed by comparing the HPLC profiles of native mr3e and its two regioselectively folded isoforms. This is the first report of three different patterns of disulfide connectivity in conotoxins with the same cysteine framework.

Published
2006
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