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156 results on '"Ji, Pengyun"'

7. Local Community Detection Algorithm Based on Core Area Expansion

Author

Ji, Pengyun, Guo, Kun, Yu, Zhiyong, Filipe, Joaquim, Editorial Board Member, Ghosh, Ashish, Editorial Board Member, Prates, Raquel Oliveira, Editorial Board Member, Zhou, Lizhu, Editorial Board Member, Sun, Yuqing, editor, Lu, Tun, editor, Cao, Buqing, editor, Fan, Hongfei, editor, Liu, Dongning, editor, Du, Bowen, editor, and Gao, Liping, editor

Published
2022
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8. Localization and Molecular Cloning of the ASMT Gene for Melatonin Synthesis in Pigs.

Author

Yan, Laiqing, Li, Guangdong, Deng, Shoulong, Wang, Likai, Wang, Yiwei, Shen, Zixia, Yin, Depeng, Ji, Pengyun, Wang, Bingyuan, and Liu, Guoshi

Subjects
MOLECULAR cloning, X chromosome, GENETIC code, ANIMAL cloning, GENOMES
Abstract

Melatonin is synthesized in multiple tissues and organs of pigs, and existing studies have shown the presence of the melatonin-synthesizing enzyme ASMT protein. However, the genomic information for the ASMT gene has been lacking. The aim of this study was to locate the genomic information of the ASMT gene in pigs using comparative genomics analysis and then obtain the coding region information through molecular cloning. First, using the NCBI Genome Data Viewer, we found that in most animals, the AKAP17A gene is often located next to the ASMT gene, with both genes arranged in the same direction. Similarly, the P2RY8 gene is commonly adjacent to the ASMTL gene, also in the same orientation. We also discovered that the ASMTL gene is frequently adjacent to the ASMT gene and arranged in the opposite direction. Using the "three-point localization" principle, we inferred the position of the ASMT gene based on the coordinates of AKAP17A and ASMTL in pigs. Our results revealed that on the pig X chromosome, a gene called LOC110258194 is located next to the AKAP17A and ASMTL genes, and its arrangement aligns with the ASMT gene in other species. Additionally, Ensembl contains a gene, ENSSSCG00000032659, at the same position, with completely overlapping exons, though it is not annotated as ASMT. Further analysis using the TreeFam tool from EMBL-EBI and the CDD tool from NCBI revealed that LOC110258194 and ENSSSCG00000032659 do not contain the typical Maf domain of ASMTL and, thus, should not be annotated as ASMTL, but rather as the ASMT gene. Using a slow-down PCR method for high-GC content genes, we successfully cloned the full CDS region of the pig ASMT gene and identified a new transcript missing Exon 6 and Exon 7. This transcript was submitted to NCBI and assigned the GenBank accession number MW847601. Our results represent the first successful localization of the ASMT gene in pigs, the first cloning of the ASMT gene's coding region, and the first discovery of a new transcript of the pig ASMT gene. [ABSTRACT FROM AUTHOR]

Published
2025
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9. The Protective Effects of Melatonin on Hainan Black Goats Under Heat Stress: Understanding Its Actions and Mechanisms.

Author

Wu, Hao, Qin, Baochun, Yang, Guang, Ji, Pengyun, Gao, Yu, Zhang, Lu, Wang, Bingyuan, and Liu, Guoshi

Subjects
GUT microbiome, CLIMATE change, GOATS, HIGH temperatures, OXIDATIVE stress
Abstract

As the global climate changes, high temperatures will cause heat stress, which significantly affects the productive efficiency of livestock. Currently, there is a lack of efficient methods to use in targeting this issue. In this study, we report that melatonin supplementation may represent an alternative method to reduce the negative impact of heat stress on livestock, particularly in Hainan black goats. Our results show that melatonin treatment increased the average daily gain of Hainan black goats that were exposed to constantly high temperatures for two months compared to controls. Our mechanistic exploration revealed that melatonin treatment not only reduced the oxidative stress and inflammatory reaction caused by heat stress but also improved goats' metabolic capacity, promoting their growth and development. More importantly, for the first time, we observed that melatonin treatment modified the abundance of the intestinal microflora, altering the metabolism of the goats, which further improved their tolerance to constant heat stress. [ABSTRACT FROM AUTHOR]

Published
2025
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10. Transcriptome Analysis Reveals Sertoli Cells Adapting Through Redox and Metabolic Pathways Under Heat Stress in Goats.

Author

Yang, Guang, Wang, Yiwei, Ji, Pengyun, Wang, Bingyuan, and Liu, Guoshi

Subjects
SERTOLI cells, TESTIS physiology, ANIMAL culture, GENE expression profiling, OXIDATIVE stress
Abstract

Background/Objectives: Climate change-induced temperature elevations pose significant challenges to livestock reproduction, particularly affecting testicular function in small ruminants. This study investigates the acute heat-stress response in goat Sertoli cells (SCs), aiming to elucidate the molecular mechanisms underlying heat-induced damage to male reproductive tissues. Methods: SCs were isolated from testes of 4-month-old black goats and exposed to heat stress (44 °C for 2.5 h). We employed transcriptome sequencing, CCK-8 assay, electron microscopy, ROS measurement, autophagy detection, Western blot analysis, and lactate concentration measurement. Bioinformatics analyses including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and protein–protein interaction network analyses were performed on the transcriptome data. Results: Heat stress significantly reduced SC viability, induced oxidative stress and autophagy, and altered gene expression profiles. We identified 1231 significantly differentially expressed genes, with significant enrichment in membrane-related processes and metabolic pathways. Metabolism-related genes, including PKLR, ACOT11, and LPCT12, were significantly downregulated. Protein–protein interaction network analysis revealed ten hub genes potentially crucial in the heat-stress response: HSP90AA1, HSPA5, BAG3, IGF1, HSPH1, IL1A, CCL2, CXCL10, ALB, and CALML4. Conclusions: This study provides comprehensive insights into the molecular mechanisms underlying goat SC response to heat stress. The identified genes and pathways, particularly those related to metabolism and stress response, offer potential targets for developing strategies to mitigate heat-stress effects on livestock reproduction. These findings contribute to our understanding of climate change impacts on animal husbandry and may inform the development of heat-stress resistant livestock lines. [ABSTRACT FROM AUTHOR]

Published
2024
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15. Identification of Site in the UTY Gene as Safe Harbor Locus on the Y Chromosome of Pig.

Author

Chen, Xiaomei, Yang, Guang, Ji, Pengyun, Liu, Guoshi, and Zhang, Lu

Subjects
GENE expression, Y chromosome, SAFE harbor, CRISPRS, LOCUS (Genetics)
Abstract

Genomic Safe Harbors (GSH) are loci used for the insertion of exogenous genetic elements, enabling exogenous gene expressing predictably without alterations of the host genome. These sites are becoming increasingly important as the gene editing technologies advance rapidly. Currently, only a few GSHs have been identified in the pig genome. In this study, a novel strategy was demonstrated for the efficient insertion of exogenous genetic material into the third exon of the UTY gene on the Y chromosome using CRISPR/Cas9-mediated homology arm-mediated end joining. The safety of the locus was verified according to the proper expression of the inserted EGFP gene without altering the expression of UTY. This approach enables the integration and expression of the exogenous gene at this locus, indicating that the UTY locus serves as a genomic safe harbor site for gene editing in the pig genome. Located on the Y chromosome, this site can be utilized for sex-biased pig breeding and developing biomedical models. [ABSTRACT FROM AUTHOR]

Published
2024
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16. Mitochondria of Porcine Oocytes Synthesize Melatonin, Which Improves Their In Vitro Maturation and Embryonic Development.

Author

Zhu, Tianqi, Yan, Laiqing, Deng, Shoulong, Ma, Wenkui, Xia, Fan, Wang, Likai, Ma, Xiao, Li, Guangdong, Shen, Zixia, Wang, Yiwei, Fu, Yao, Ji, Pengyun, Wang, Bingyuan, Zhang, Lu, and Liu, Guoshi

Subjects
EMBRYOLOGY, ELECTRON transport, MEMBRANE potential, OXIDATIVE phosphorylation, OVUM, MITOCHONDRIAL membranes
Abstract

The in vitro maturation efficiency of porcine oocytes is relatively low, and this limits the production of in vitro porcine embryos. Since melatonin is involved in mammalian reproductive physiology, in this study, we have explored whether endogenously produced melatonin can help in porcine oocyte in vitro maturation. We have found, for the first time in the literature, that mitochondria are the major sites for melatonin biosynthesis in porcine oocytes. This mitochondrially originated melatonin reduces ROS production and increases the activity of the mitochondrial respiratory electron transport chain, mitochondrial biogenesis, mitochondrial membrane potential, and ATP production. Therefore, melatonin improves the quality of oocytes and their in vitro maturation. In contrast, the reduced melatonin level caused by siRNA to knockdown AANAT (siAANAT) is associated with the abnormal distribution of mitochondria, decreasing the ATP level of porcine oocytes and inhibiting their in vitro maturation. These abnormalities can be rescued by melatonin supplementation. In addition, we found that siAANAT switches the mitochondrial oxidative phosphorylation to glycolysis, a Warburg effect. This metabolic alteration can also be corrected by melatonin supplementation. All these activities of melatonin appear to be mediated by its membrane receptors since the non-selective melatonin receptor antagonist Luzindole can blunt the effects of melatonin. Taken together, the mitochondria of porcine oocytes can synthesize melatonin and improve the quality of oocyte maturation. These results provide an insight from a novel aspect to study oocyte maturation under in vitro conditions. [ABSTRACT FROM AUTHOR]

Published
2024
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17. Melatonin improves the vitrification of sheep morulae by modulating transcriptome

Author

Ji, Pengyun, primary, Liu, Yunjie, additional, Yan, Laiqing, additional, Jia, Yanquan, additional, Zhao, Mengmeng, additional, Lv, Dongying, additional, Yao, Yujun, additional, Ma, Wenkui, additional, Yin, Depeng, additional, Liu, Fenze, additional, Gao, Shuai, additional, Wusiman, Abulizi, additional, Yang, Kailun, additional, Zhang, Lu, additional, and Liu, Guoshi, additional

Published
2023
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23. Melatonin Supplementation during the Late Gestational Stage Enhances Reproductive Performance of Sows by Regulating Fluid Shear Stress and Improving Placental Antioxidant Capacity

Author

Wang, Likai, primary, Yan, Laiqing, additional, Han, Qi, additional, Li, Guangdong, additional, Wu, Hao, additional, Ma, Xiao, additional, Zhao, Mengmeng, additional, Ma, Wenkui, additional, Ji, Pengyun, additional, Zhang, Ran, additional, and Liu, Guoshi, additional

Published
2023
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25. Melatonin promotes the growth and development of lambs by increasing growth hormone and testosterone, targeting on apoptosis signaling pathway and intestinal microflora

Author

Ma, Wenkui, primary, Wu, Hao, additional, Li, Guangdong, additional, Yan, Laiqing, additional, Wang, Likai, additional, Zhao, Mengmeng, additional, Guan, Shengyu, additional, Xu, Shang, additional, Guo, Xiaokai, additional, Liu, Fenze, additional, Ji, Pengyun, additional, Wusiman, Abulizi, additional, and Liu, Guoshi, additional

Published
2022
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26. Melatonin promotes the development of sheep transgenic cloned embryos by protecting donor and recipient cells

Author

Yao, Yujun, primary, Yang, Ailing, additional, Li, Guangdong, additional, Wu, Hao, additional, Deng, Shoulong, additional, Yang, Hai, additional, Ma, Wenkui, additional, Lv, Dongying, additional, Fu, Yao, additional, Ji, Pengyun, additional, Tan, Xinxing, additional, Zhao, Wanmin, additional, Lian, Zhengxing, additional, Zhang, Lu, additional, and Liu, Guoshi, additional

Published
2022
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27. Additional file 6 of Whole-genome methylation analysis reveals epigenetic variation between wild-type and nontransgenic cloned, ASMT transgenic cloned dairy goats generated by the somatic cell nuclear transfer

Author

Wu, Hao, Zhou, Wendi, Liu, Haijun, Cui, Xudai, Ma, Wenkui, Wu, Haixin, Li, Guangdong, Wang, Likai, Zhang, Jinlong, Zhang, Xiaosheng, Ji, Pengyun, Lian, Zhengxing, and Liu, Guoshi

Abstract

Additional file 6: Fig. S6. mCpG bits methylation rate distribution frequency graph (A, ASMT transgenic cloned goat (K2020); B, control goat (S2); C, Nontransgenic cloned goat (K03)).

Published
2022
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28. Additional file 5 of Whole-genome methylation analysis reveals epigenetic variation between wild-type and nontransgenic cloned, ASMT transgenic cloned dairy goats generated by the somatic cell nuclear transfer

Author

Wu, Hao, Zhou, Wendi, Liu, Haijun, Cui, Xudai, Ma, Wenkui, Wu, Haixin, Li, Guangdong, Wang, Likai, Zhang, Jinlong, Zhang, Xiaosheng, Ji, Pengyun, Lian, Zhengxing, and Liu, Guoshi

Abstract

Additional file 5: Fig. S5. mCpG bits cover the depth distribution frequency map (A, ASMT transgenic cloned goat (K2020); B, control goat (S2); C, Nontransgenic cloned goat (K03)).

Published
2022
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32. Overexpression of ASMT likely enhances the resistance of transgenic sheep to brucellosis by influencing immune‐related signaling pathways and gut microbiota

Author

Li, Guangdong, primary, Lv, Dongying, additional, Yao, Yujun, additional, Wu, Hao, additional, Wang, Jing, additional, Deng, Shoulong, additional, Song, Yukun, additional, Guan, Shengyu, additional, Wang, Likai, additional, Ma, Wenkui, additional, Yang, Hai, additional, Yan, Laiqing, additional, Zhang, Jinlong, additional, Ji, Pengyun, additional, Zhang, Lu, additional, Lian, Zhengxing, additional, and Liu, Guoshi, additional

Published
2021
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36. Melatonin promotes male reproductive performance and increases testosterone synthesis in mammalian Leydig cells

Author

Yang, Minghui, primary, Guan, Shengyu, additional, Tao, Jingli, additional, Zhu, Kuanfeng, additional, Lv, Dongying, additional, Wang, Jing, additional, Li, Guangdong, additional, Gao, Yuefeng, additional, Wu, Hao, additional, Liu, Jinghao, additional, Cao, Lin, additional, Fu, Yao, additional, Ji, Pengyun, additional, Lian, Zhengxing, additional, Zhang, Lu, additional, and Liu, Guoshi, additional

Published
2021
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38. α‐ketoglutarate delays age‐related fertility decline in mammals

Author

Zhang, Zhenzhen, primary, He, Changjiu, additional, Gao, Yu, additional, Zhang, Lu, additional, Song, Yukun, additional, Zhu, Tianqi, additional, Zhu, Kuanfeng, additional, Lv, Dongying, additional, Wang, Jing, additional, Tian, Xiuzhi, additional, Ma, Teng, additional, Ji, Pengyun, additional, Cui, Wei, additional, and Liu, Guoshi, additional

Published
2021
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41. NLRP7 is expressed in the ovine ovary and associated with in vitro pre-implantation embryo development

Author

Li, Guangdong, primary, Tian, Xiuzhi, additional, Lv, Dongying, additional, Zhang, Lu, additional, Zhang, Zhenzhen, additional, Wang, Jing, additional, Yang, Minghui, additional, Tao, Jingli, additional, Ma, Teng, additional, Wu, Hao, additional, Ji, Pengyun, additional, Wu, Yingjie, additional, Lian, Zhengxing, additional, Cui, Wei, additional, and Liu, Guoshi, additional

Published
2019
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45. Effects of AANAT overexpression on the inflammatory responses and autophagy activity in the cellular and transgenic animal levels

Author

Tao, Jingli, primary, Yang, Minghui, additional, Wu, Hao, additional, Ma, Teng, additional, He, Changjiu, additional, Chai, Menglong, additional, Zhang, Xiaosheng, additional, Zhang, Jinlong, additional, Ding, Fangrong, additional, Wang, Sutian, additional, Deng, Shoulong, additional, Zhu, Kuanfeng, additional, Song, Yukun, additional, Ji, Pengyun, additional, Liu, Haijun, additional, Lian, Zhengxing, additional, and Liu, Guoshi, additional

Published
2018
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48. RNAi combining Sleeping Beauty transposon system inhibits ex vivo expression of foot-and-mouth disease virus VP1 in transgenic sheep cells

Author

Deng, Shoulong, primary, Li, Guangdong, additional, Yu, Kun, additional, Tian, Xiuzhi, additional, Wang, Feng, additional, Li, Wenting, additional, Jiang, Wuqi, additional, Ji, Pengyun, additional, Han, Hongbing, additional, Fu, Juncai, additional, Zhang, Xiaosheng, additional, Zhang, Jinlong, additional, Liu, Yixun, additional, Lian, Zhengxing, additional, and Liu, Guoshi, additional

Published
2017
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