Objective: To explore the regularity of distribution of press-sensitive acupoints on the body surface in tinnitus patients, so as to provide evidence for selecting suitable acupuncture points in the treatment of tinnitus., Methods: Sixty-three cases of tinnitus patients (from 25 to 75 years in age) were recruited in the present study. The patient was asked to take a sitting, supine and prone position, successively, and the operator used the thumb to moderately press the subject's head, neck, back, four limbs below the knee- and elbow joints along the three Hand- and three Foot- yang and yin meridians. Those acupoints that appeared pressing pain, or soreness-distension, numbness, or radiation feeling more than 10 times were brought into the sequence analysis by using the Excel 2010., Results: A total of 131 press-sensitive acupoints including 41 points with an appea-rance frequency of more than 10 times were found in the 63 tinnitus patients. These 41 acupoints distributing in the top three meri-dians were the Gallbladder Meridian of Foot-Shaoyang, Tri-energizer Meridian of Hand-Shaoyang and Spleen Meridian of Foot-Taiyin, and their involved regions were those below the knee-joints, bilateral sides of the head and marginal portion of the ear, sites bellow the elbow-joints, back-waist, successively in the appearance frequency. The top ten acupoints are Wangu (GB 12), Yifeng (SJ 17), Waiguan (SJ 5), Quchi (LI 11), Sanyinjiao (SP 6), Yanglingquan (GB 34), Fengchi (GB 20), Taichong (LR 3), Shousanli (LI 10) and Zulinqi (GB 41), in sequence., Conclusions: The press-sensitive acupoints in tinnitus patients distribute principally in the Gallbladder Meridian of Foot-Shaoyang, and below the knee-joint, and the most sensitive acupoints are Wangu (GB 12) and Yifeng (SJ 17). These findings may provide an objective basis for clinical diagnosis and treatment of tinnitus.