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Satellite cells are required for muscle regeneration, remodeling, and repair through their activation, proliferation, and differentiation; however, how dietary factors regulate this process remains poorly understood. The L-type amino acid transporter 1 (LAT1) transports amino acids, such as leucine, into mature myofibers, which then stimulate protein synthesis and anabolic signaling. However, whether LAT1 is expressed on myoblasts and is involved in regulating myogenesis is unknown. The aim of this study was to characterize the expressional and functional relevance of LAT1 during different stages of myogenesis and in response to growth and atrophic conditions in vitro. We determined that LAT1 is expressed by C2C12 and human primary myoblasts, and its gene expression is lower during differentiation ( P < 0.05). Pharmacological inhibition and genetic knockdown of LAT1 impaired myoblast viability, differentiation, and fusion (all P < 0.05). LAT1 protein content in C2C12 myoblasts was not significantly altered in response to different leucine concentrations in cell culture media or in two in vitro atrophy models. However, LAT1 content was decreased in myotubes under atrophic conditions in vitro ( P < 0.05). These findings indicate that LAT1 is stable throughout myogenesis and in response to several in vitro conditions that induce muscle remodeling. Further, amino acid transport through LAT1 is required for normal myogenesis in vitro.

Carbohydrates are ubiquitous in nature and present across all kingdoms of life – bacteria, fungi, viruses, yeast, plants, animals and humans. They are essential to many biological processes. However, due to their complexity and heterogeneous nature they are often neglected, sometimes referred to as the ‘dark matter’ of biology. Nevertheless, due to their extensive biological impact on health and disease, glycans and the field of glycobiology have become increasingly popular in recent years, giving rise to glycan-based drug development and therapeutics. Forecasting of communicable diseases predicts that we will see an increase in pandemics of humans and livestock due to global loss of biodiversity from changes to land use, intensification of agriculture, climate change and disruption of ecosystems. As such, the development of point-of-care devices to detect pathogens is vital to prevent the transmission of infectious disease, as we have seen with the COVID-19 pandemic. So, can glycans be exploited to detect COVID-19 and other infectious diseases? And is this technology sensitive and accurate? Here, I discuss the structure and function of glycans, the current glycan-based therapeutics and how glycan binding can be exploited for detection of infectious disease, like COVID-19.

Professional counselors are becoming increasingly represented in the Primary Care Behavioral Health (PCBH) workforce. Accordingly, models for training these professionals to work in integrated health care settings are needed.This article presents the results of 34 master's level counseling students' self-reported competencies in PCBH as a result of a PCBH training program.Results indicated that students' self-perceived competence across all domains increased over time.Implications for the function of self-reported skill development in students training as behavioral health consultants in primary care are discussed. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

OBJECTIVES Patients speaking a primary language other than English face barriers to equitable care, particularly patient–provider communications. There is no gold standard for providing inpatient medical interpretation on family-centered rounds (FCR). We aimed to implement simultaneous, in-person interpretation of FCR for Spanish-speaking families and hypothesized improved satisfaction in care. METHODS In-person, Spanish Equipment-Assisted Simultaneous Medical Interpretation (EASMI) was implemented in March 2018 on FCR. Child Hospital Consumer Assessment of Healthcare Providers and Systems (Child HCAHPS) experience scores on communication domains were analyzed for Spanish and English-speaking families pre- (n = 118) and postimplementation (n = 552). Postimplementation, we conducted medical team surveys (n = 104) and semistructured interviews with Spanish-speaking families (n = 25) to determine satisfaction with interpretation modalities (phone, video, and EASMI). RESULTS Spanish-speaking families exhibited statistically significant improvements in Child HCAHPS top box scores compared to English-speaking families in multiple communication and informed care-related domains. For example, "How often did your child’s doctors explain things to you in a way that was easy to understand?" top box scores improved from 58% to 95% for Spanish-speaking families, compared to 85% to 83% for English speakers, with the differential effect of the intervention showing statistical significance (P = .001). Medical team surveys demonstrated high satisfaction with EASMI. Qualitative themes from interviews and open-ended survey responses emphasized multiple care benefits with EASMI, including a perceived reduction of communication errors and increased family participation. CONCLUSIONS EASMI was associated with significant improvements in Child HCAHPS scores in communication domains and increased medical team and family members’ satisfaction with interpretation. EASMI presents a novel method for equitable FCR for Spanish-speaking families.

The United States incarceration rate is the highest in the world. Most research involving individuals with criminal involvement history focuses on predictors of recidivism versus factors contributing to success post-release. Post-release successes are important considerations to inform strengths-based approaches to working with this population. In this qualitative study, perspectives of males convicted of a violent offense in the United States who did not recidivate at least three-years-post release from incarceration were explored. Utilizing narrative inquiry, six participants shared their stories of how they avoided returning to a correctional facility. Through narrative analysis, the researchers identified five themes across participant stories: (a) knowledge as power, (b) paychecks after prison, (c) using to belong, (d) connections, and (e) transformational thinking. This study provides a strengths-based perspective when working with formerly incarcerated persons (FIPs). Implications for counselors and counselor educators are provided.

This narrative inquiry presents seven Hispanic counselors in long-term practice in the Rio Grande Valley (RGV) of Texas and their stories of professional resilience in a health professional shortag...

Stepfamilies are the most common family structure in the United States. Many graduate and professional training programs provide little attention to the unique stressors that stepfamilies face, as ...

Radiation exposure during muscle development induces long-term decrements to skeletal muscle health, which contribute to reduced quality of life in childhood cancer survivors. Whether the effects of radiation on skeletal muscle are influenced by relevant physiological factors, such as obesity and exercise training remains unknown. Using skeletal muscle from our previously published work examining the effects of obesity and exercise training on radiation-exposed bone marrow, we evaluated the influence of these physiological host factors on irradiated skeletal muscle morphology and cellular dynamics. Mice were divided into control and high fat diet groups with or without exercise training. All mice were then exposed to radiation and continued in their intervention group for an additional 4 weeks. Diet-induced obesity resulted in increased muscle fibrosis, while obesity and exercise training both increased muscle adiposity. Exercise training enhanced myofibre cross-sectional area and the number of satellite cells committed to the myogenic lineage. High fat groups demonstrated an increase in p-NFĸB expression, a trend for a decline in IL-6, and increase in TGFB1. These findings suggest exercise training improves muscle morphology and satellite cell dynamics compared to diet-induced obesity in irradiated muscle, and have implications for exercise interventions in cancer survivors.

INTRODUCTION Radiation therapy increases the risk of secondary malignancy and morbidity in cancer survivors. The role of obesity and exercise training in modulating this risk is not well understood. As such, we used a preclinical model of radiation-induced malignancy to investigate whether diet-induced obesity and/or endurance exercise training altered lifelong survival, cancer incidence, and morbidity. METHODS Male CBA mice were randomly divided into control diet/sedentary group (CTRL/SED), high-fat diet (45% fat)/sedentary group (HFD/SED), control diet/exercise group (2-3 d·wk-1; CTRL/EX), or high-fat diet/exercise group (HFD/EX) groups then exposed to whole-body radiation (3 Gy). End point monitoring and pathology determined mortality and cancer incidence, respectively. Health span index, a measure of morbidity, was determined by a composite measure of 10 anthropometric, metabolic, performance, and behavioral measures. RESULTS Overall survival was higher in HFD/SED compared with CTRL/SED (P < 0.05). The risk of cancer-related mortality by 18 months postradiation was 1.99 and 1.63 in HFD/SED compared with CTRL/EX (RR = 1.99, 95% confidence interval = 1.20-3.31, P = 0.0081) and CTRL/SED (RR = 1.63, 95% confidence interval = 1.06-2.49, P = 0.0250), respectively. The number of mice at end point with cancer was higher in HFD/SED compared with CTRL/EX and CTRL/SED (P < 0.05). Health span index was highest in CTRL/EX (score = +2.5), followed by HFD/EX (score = +1), and HFD/SED (score = -1) relative to CTRL/SED. CONCLUSION This work provides the basis for future preclinical studies investigating the dose-response relationship between exercise training and late effects of radiation therapy as well as the mechanisms responsible for these effects.

Weight loss and exercise reduce colorectal cancer (CRC) risk in persons with obesity. Whether weight loss and exercise effect myofibre characteristics and muscle stem/progenitor cell populations in mice with preneoplastic colorectal lesions, a model of CRC risk, is unknown. To address this gap, male C57Bl/6J mice were fed a high-fat diet (HFD) to induce obesity or a control (CON) diet prior to azoxymethane injection to induce preneoplastic colorectal lesions. The HFD group was then randomized to weight loss conditions that included (1) switching to the CON diet only (HFD-SED) or switching to the CON diet with treadmill exercise training (HFD-EX). Average myofibre cross-sectional area was not different between groups. There were more smaller-sized fibers in HFD-EX (p

Chronic wounds represent a growing clinical problem for which limited treatment strategies exist. Defects in immune cell-mediated healing play an important role in chronic wound development, presenting an attractive clinical target in the treatment of chronic wounds. However, efforts to improve healing through the application of growth factors and cytokines have been limited by the rapid degradation and diffusion of these molecules in the wound environment. In this study we sought to overcome the challenge of rapid diffusion through the development of a hydrogel delivery system in which protein cargo can be released into the wound environment at a constant and tunable rate. This system was used to deliver the intercellular adhesion molecule-1 (ICAM-1) in order to target endogenous cells upstream of growth factor and cytokine production and circumvent the issue of their rapid degradation. We demonstrated that our delivery system was able to release cargo at different and highly controllable rates and thereby improved cargo retention in the wound environment. Additionally, treatment with ICAM-1 in the delivery system improved healing in both ICAM-1-deficient mice and an aged mouse model of delayed healing, highlighting a potential clinical benefit for this protein in the treatment of chronic wounds.

Counselors face increasing pressure to demonstrate accountability for client and agency outcomes. Despite persistent calls to embrace research and assessment, program evaluation remains relatively ...

Mentoring is a professional leadership contribution of counselor educators resulting in personal and vocational benefits for students. A need to understand research-informed mentoring from the ment...

Confidentiality is integral to counseling relationships, particularly for adolescent clients. The presence of “serious and foreseeable harm” (American Counseling Association [ACA], 2014, p. 5) may ...

The 2014 ACA Code of Ethics (American Counseling Association, 2014) distinguishes between competence-based and value-based referrals. This phenomenological research explored student counselors' (N = 10) perceptions of client referrals across a range of cognitive developmental positions. Findings indicated nuanced understandings of competence, values, referral processes, responsibilities of supervisors, and learning environments. Implications for counselor education and supervision are discussed.

Professional identity is characterized by the integration of individuals' professional and personal selves. The authors used a multiple regression design to examine counselor professional ethical identity development, with specific attention to moral and intellectual development. Student counselors (N = 59) completed the Modified Subject-Object Interview (Monson & Hamilton, 2010), Defining Issues Test–2 (Rest, Narvaez, Bebeau, & Thoma, 1999a), and Learning Environment Preferences scale (Moore, 1987). Moral development was the most significant predictor of professional ethical identity development. Preparatory and research strategies to enhance professional ethical identity formation are discussed.

Background: To date, no study has examined the effectiveness of social distancing, while controlling for social mobility and social distancing restrictions in the United States. We utilize the quasi-experimental setting created by the nationwide protests precipitated by George Floyd’s tragic death on May 25, 2020, to assess the causal impact of social distancing on the spread of SARS-CoV-2. Methods: Our sample period spans from January 22, 2020, to June 20, 2020, and consists of 474,422 county-days representing 3,142 counties from all 50 states and the District of Columbia. To assess the change in COVID-19 case counts following the protests, we employ a differences-in-differences estimation strategy in a multivariate setting, in which we control for social distancing restrictions and social mobility across counties. We also control for covariates that may influence SARS-CoV-2 transmission, and implement placebo tests using a Monte Carlo simulation. Findings: We document a country-wide increase of over 3 · 06 cases per day, per 100,000 population, following the onset of the protests (95%CI: 2 · 47 – 3 · 65 ), and a further increase of 1 · 73 cases per day, per 100,000 population, in the counties in which the protests took place (95%CI: 0 · 59 – 2 · 87 ). Relative to the week preceding the onset of the protests, this represents a 61 · 2 % country-wide increase in the infection rate, and a further 34 · 6 % increase in the protest counties. Interpretation: Our study documents a significant increase in COVID-19 case counts in counties that experienced a protest, and we conclude that social distancing practices causally impact the spread of SARS-CoV-2. The observed effect cannot be explained by changes in social distancing restrictions and social mobility, and placebo tests rule out the possibility that this finding is attributable to chance. Funding: We acknowledge the financial support from the Smith School of Business Distinguished Faculty Fellowship at Queen’s University. Declaration of Interests: Authors declare no conflict of interest.

Background Accurate assessment of surgical‐site infection (SSI) is crucial for surveillance and research. Self‐reporting patient measures are needed because current SSI tools are limited for assessing patients after leaving hospital. The Bluebelle Wound Healing Questionnaire (WHQ) was developed for patient or observer completion; this study tested its acceptability, scale structure, reliability and validity in patients with closed primary wounds after abdominal surgery. Methods Patients completed the WHQ (self‐assessment) within 30 days after leaving hospital and returned it by post. Healthcare professionals completed the WHQ (observer assessment) by telephone or face‐to‐face. Questionnaire response rates and patient acceptability were assessed. Factor analysis and Cronbach's α examined scale structure and internal consistency. Test–retest and self‐ versus observer reliability assessments were performed. Sensitivity and specificity for SSI discrimination against a face‐to‐face reference diagnosis (using Centers for Disease Control and Prevention criteria) were examined. Results Some 561 of 792 self‐assessments (70·8 per cent) and 597 of 791 observer assessments (75·5 per cent) were completed, with few missing data or problems reported. Data supported a single‐scale structure with strong internal consistency (α greater than 0·8). Reliability between test–retest and self‐ versus observer assessments was good (κ 0·6 or above for the majority of items). Sensitivity and specificity for SSI discrimination was high (area under the receiver operating characteristic (ROC) curve 0·91). Conclusion The Bluebelle WHQ is acceptable, reliable and valid with a single‐scale structure for postdischarge patient or observer assessment of SSI in closed primary wounds., Simple and valid

Acts of bullying and interpersonal violence have grave public health implications. Existing anti-bullying and interpersonal violence interventions rely on scripted, solution-focused protocols that lack relational shifts necessary for lasting change. The Impact Project is a grassroots campaign sponsored in conjunction with President Duffey’s 2015–2016 American Counseling Association Presidential Initiative on Anti-Bullying and Interpersonal Violence. The Impact Project is framed by relational cultural theory (RCT) and aims to help combat bullying and interpersonal violence by publicly honoring those who may not know or understand their value. This article addresses the mental and public health implications of bullying and interpersonal violence and the application of RCT to developing integrated community interventions. Recommendations for cultivating creative spaces, promoting resilience, and continuing the “impact” are provided.

School counseling program directors hold unique positions as both administrators and school counselors. Research to date has not explored the status of professional school counseling from the persp...

Background Language barriers significantly contribute to medical errors compromising patient safety, quality of care, and resident engagement with patients. Spanish speakers account for the fastest growing population of patients within US pediatric hospitals. However, the communication needs of limited English proficiency (LEP) patients frequently remain unmet. PURPOSE: The purpose of this study is to implement equipment assisted simultaneous medical interpretation (EASMI) for Spanish speaking families on family centered bedside rounds (FCR) and determine family and trainee provider satisfaction. Methods In-person interpreters joined FCR at a tertiary care academic children's hospital. All medical discussion on FCR was interpreted simultaneously from English to Spanish. Interpreters wore a noise-cancelling microphone connected to a transmitter and family members were given a receiver with an earpiece. Qualitative interviews were performed with family members on their experience (N=25). Online qualitative and quantitative surveys were completed by medical students, residents, fellows, attendings, and nurses (N= 136). Results Surveys demonstrated 94% of physicians and 100% of nurses were “very satisfied” with EASMI. Common themes from the resident and fellow survey were improved efficiency of rounds, increased family engagement, improved understanding of the care plan, decreased communication errors and improving patient safety. After the intervention Spanish speaking families reported an increase in the domains of communication with physicians and nurses on the Child HCAHPS survey. Conclusion Medical interpretation for LEP patients and families is critical for improved healthcare and reduction in health disparities. Overall, EASMI is both an efficient and effective patient-centered intervention during pediatric FCR for increased trainee engagement with patients.

The phenotyping system UPOINT has proven effective in classifying patients with Urologic Pelvic Pain Syndromes in a clinically meaningful way and to guide therapy. While highly successful in men with chronic pelvic pain syndrome (CPPS), UPOINT is more limited in patients with interstitial cystitis/painful bladder syndrome (IC/PBS) since by definition all patients have the urinary and organ specific phenotype. Furthermore, AUA guidelines recommend a sequential tiered approach to therapy rather than the multimodal UPOINT scheme. We sought to modify UPOINT to be more practical and efficacious for IC/PBS.We developed a new phenotype by removing the urinary and organ specific domains from UPOINT and adding a Hunner's ulcers (U) domain, since these patients benefit from phenotype specific therapies (fulguration, cyclosporine). This yields 'INPUT': infection, neurologic/systemic, psychosocial, ulcers and tenderness of muscles. We applied this system retrospectively to our previously validated upointmd.com IC/PBS database. Symptoms were measured by the Genitourinary Pain Index (GUPI) (valid for men and women). The database was searched for patients with complete data to assess the INPUT domains and include GUPI. Men were included if they reported pain relieved by voiding and/or presence of Hunner's ulcers. Groups were compared with ANOVA, Mann-Whitney, t test or chi squared when appropriate and correlated with Spearman r.There were 239 patients, 154 female (64%) with age range 18-79 (mean 41.8). Incidence of domains was infection 11%, neurologic/systemic 51%, psychosocial 81%, ulcers 18% and tenderness 85%. Mean total domains was 2.46 (range 0-5) and 65% had 2 or 3 positive domains while only 5% had none. There was a stepwise increase in GUPI score with increasing number of positive INPUT domains (ANOVA for differences between groups p0.0001. Correlation by Spearman r = 0.355 p0.0001). Presence of Hunner's ulcers increased mean symptom score (25.7 versus 29.7, p = 0.004) and indeed each of the domains significantly increased total GUPI score except for Infection.The INPUT phenotype in IC/PBS appears to replicate the validity and potential clinical utility of UPOINT in CPPS. Patients have a diversity of phenotypes and more positive domains correlate with more severe symptoms. Since 95% of patients have at least 1 positive domain it may benefit patients to receive multimodal therapy up front for these extra domains (eg. pelvic floor physical therapy, fulguration of ulcers) rather than relying on a sequential tiered approach.

Motivational interviewing (MI) is an evidence-based, client-centered approach that provides a framework for the change process. Careful attention is given within MI to understand and meet clients across a continuum of readiness for change. While less readily applied to family counseling, MI facilitates therapeutic alliance, evokes motivation for change, and provides useful guidelines for conceptualizing and implementing family counseling services. This article (a) provides a brief overview of MI’s major tenets, (b) examines systems-level applications of MI principles, and (c) describes five counseling interventions developed by the authors that illustrate how MI may be integrated into family counseling practice.

This article reports the findings of a study that examined why families choose to return to family therapy after their first family therapy session. The 87 families that were referred to a university family counseling center for assistance with their children’s behavioral problems at school completed an author-developed survey in which they rated the influence of various therapist-related and family-related factors on their decision to return for a second session. Factor analysis of the survey data revealed that the families’ positive experience of the therapist during the first session had the strongest influence on their continuation decision. The findings suggest that family therapists are not helpless victims to client attrition after the first session, but rather, have the strongest potential for influencing client decisions to come back. Specific family therapist actions for maximizing family continuation after the first session are described.

A purposeful sample of 359 graduate counseling students completed a survey assessing factors influencing program enrollment decisions with particular attention to students’ awareness of and importance ascribed to accreditation from the Council for Accreditation of Counseling and Related Educational Programs (CACREP) prior to and following enrollment. Results indicated that accreditation was the second most influential factor in one half of the students’ enrollment decisions; nearly half of participants were unaware of CACREP accreditation prior to enrollment. Accreditation was a top factor that students attending non-CACREP-accredited programs wished they had considered more in their enrollment decisions. Findings from the survey indicate that prospective counseling students often lack necessary information regarding accreditation that may influence enrollment decisions. Implications for counseling students and their graduate preparation programs, CACREP and the broader counseling profession are discussed.

Background: An evidence-based trauma-informed resilience skills training program developed for deployed military personnel was adapted and pilot-tested with pediatric residents. We anticipated high satisfaction ratings and changes in knowledge, beliefs, and self-efficacy related to coping with stress and trauma. Methods: The intervention included 6 skill-based modules covering emotion regulation, communication with angry patients and parents, reflective narrative, inspirational goal setting, problem-solving, and developing a self-care toolbox. An optional survey was administered before and after the training. Results: After training, 76% rated resilience skills as important, 60% were satisfied, and 82% indicated the training changed how they will respond to patient-related grief and trauma. They became more likely to believe attendings are affected by patient deaths and to know what helps them cope when they disagree with the medical decision making of others, more skilled in recognizing signs of stress and trauma, and more knowledgeable about evidence-based interventions.

Epidermal resident γδ T cells are key players in the wound healing response, providing growth factors and cytokines that promote keratinocyte proliferation following recognition of damaged self. These cells are known to be unresponsive in chronic wounds and as such represent a novel target for new wound healing therapies. In this study we seek to modulate the γδ T cell response in chronic wounds through the identification, characterization, and delivery of keratinocyte-expressed proteins that comprise a “wound signature” allowing γδ T cells to recognize and respond to epithelial damage. We have identified a set of target genes though RNA-seq analysis which are upregulated following wounding in mice and correspond to membrane-bound and secreted proteins available for interactions with neighboring cells. Candidate gene expression was measured following wounding of human skin tissue, and ICAM1, CRISPLD2, and HSPA8 were shown to also be significantly upregulated in human skin wound samples. Hspa8 and ICAM-1 have been validated through in vitro assays to activate γδ T cells, and these proteins have been chosen for in vivo delivery. In order to deliver these candidate proteins into in vivo wound models we have developed a hydrogel-based delivery system with a highly tunable time frame of release. This system improves the retention of protein cargo at the wounds site post-wounding in mice and is being used to administer our candidate proteins into mouse models of non-healing wounds to assess effects on acceleration of tissue repair.

In The Good Life: Wellbeing and the New Science of Altruism, Selfishness, and Immorality, Graham Music thoughtfully explores some of the most multifarious, yet essential, tensions of being human. A...

Hostile and unsafe learning environments persist for many lesbian, gay, bisexual, transgender, and questioning (LGBTQ) students. Professional school counselors are uniquely positioned to spearhead systemic initiatives to create safe and supportive schools that combat such oppression; however, current counselor education strategies may fail to adequately prepare school counseling trainees for this role. Cognitive developmental theory offers a pedagogical framework to assist counselor educators in conceptualizing school counseling trainees’ meaning-making positions and scaffolding matched interventions to enhance preparation toward LGBTQ affirmative counseling. A theoretical overview is provided, including the relationship of advanced cognitive development to school counselor competence, as well as samples of didactic, experiential, and reflection strategies counselor educators may employ to promote cognitive growth.

This dissertation focuses on fan culture in the world of professional wrestling, specifically the perspectives and experiences of female and AFAB (assigned female at birth) non-binary fans . The project explores broader themes about empowerment, female sexuality, and representation through the lens of fans of American professional wrestling, particularly World Wrestling Entertainment (WWE). The dissertation looks at the performance of self and the performance of fandom on social media sites (such as Tumblr, Twitter, and Instagram), during “live” wrestling events, as well as through fan fiction and other artistic expressions. In so doing, I explore various ideas about the ways that fans behave, interact, and connect with one another in a frequently male-dominated forum in order to grapple with larger questions about gender and performance in American society and history. I argue that non-male fans attempt to refigure the image of wrestling fan culture to include their own voices and their own presence through the use of social media and other virtual methods of connection and community-building.

Plague is a disease caused by Yersinia pestis. Septicemic and pneumonic plague have a high mortality rate if untreated. Here we describe the challenges of accurately diagnosing a nonfatal pediatric case of septicemic plague with involvement of multiple organs; to our knowledge, the first documented case of multifocal plague osteomyelitis.