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3 results on '"Jessica Alvarado"'

1. Community scientist program provides bi-directional communication and co-learning between researchers and community members

Author

Jessica Alvarado, Larkin L. Strong, Birnur Buzcu-Guven, Leonetta B. Thompson, Erica Cantu, Chelsea C. Carrier, Chiamaka D. Chukwu, Cassandra L. Harris, Luz K. Melendez, Crystal L. Roberson, Angela M. Ross, Sophia C. Russell, Pablo Sanchez, Amirali Tahanan, Blair C. Zdenek, Belinda M. Reininger, and Lorna H. McNeill

Subjects
Community engagement, community scientists, translational researchers, Texas, co-learning, Medicine
Abstract

Community involvement in research is key to translating science into practice, and new approaches to engaging community members in research design and implementation are needed. The Community Scientist Program, established at the MD Anderson Cancer Center in Houston in 2018 and expanded to two other Texas institutions in 2021, provides researchers with rapid feedback from community members on study feasibility and design, cultural appropriateness, participant recruitment, and research implementation. This paper aims to describe the Community Scientist Program and assess Community Scientists' and researchers' satisfaction with the program. We present the analysis of the data collected from 116 Community Scientists and 64 researchers who attended 100 feedback sessions, across three regions of Texas including Northeast Texas, Houston, and Rio Grande Valley between June 2018 and December 2022. Community Scientists stated that the feedback sessions increased their knowledge and changed their perception of research. All researchers (100%) were satisfied with the feedback and reported that it influenced their current and future research methods. Our evaluation demonstrates that the key features of the Community Scientist Program such as follow-up evaluations, effective bi-directional communication, and fair compensation transform how research is conducted and contribute to reducing health disparities.

Published
2024
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2. Hidrólisis enzimática de polvillo de arroz para la producción de etanol de segunda generación

Author

José Ayala-Armijos, Geanina Machuca-Loja, Paola Benítez Castrillón, and Jessica Alvarado-Cáceres

Subjects
General Medicine
Abstract

El objetivo de la presente investigación fue hidrolizar el polvillo de arroz a azucares reductores y mediante fermentación alcohólica obtener bioetanol. Se prepararon nueve soluciones de polvillo de arroz molido (8 %, 13 % y 18 %) y agua purificada e inoculados con conidios de Trichoderma spp en concentraciones (0,2 g/L, 0,4 g/L y 0,6 g/L), los cuales se incubaron a 30°C por 144 horas. Mediante el método DNS (3, 5 ácido di nitrosalisilico), se cuantificó los azúcares reductores: tratamiento A: 4,32 ± 0,08%, B: 5,27 ± 0,07%, C: 5,10 ± 0,01%, D: 5,53 ± 0,12%, E: 8,24 ± 0,06%, F: 6,37 ± 0,07%, G: 5,13 ± 0,02%, H: 7,19 ± 0,26%, I: 9,69 ± 0,18% y el etanol mediante cromatografía de gases. El tratamiento que presentó mayor porcentaje de bioconversión fue el tratamiento I (18 % y 0,6 g/L de inóculo), donde la concentración de glucosa, fue 9,88%, y el rendimiento de etanol fue de 5° GL. En conclusión, es posible hidrolizar los carbohidratos totales presente en el polvillo de arroz en azucares reductores y posterior fermentación alcohólica.

Published
2020

3. Optimizing Tuberculosis Testing for Basic Laboratories

Author

Robert H. Gilman, Rosario Montoya, Eric S. Ramos, Mark J. Siedner, Samuel G. Schumacher, Willi Quino, Jessica Alvarado, Beatriz Herrera, Louis Grandjean, Laura Martin, Carlton A. Evans, and Jonathan M. Sherman

Subjects
Adult, Male, Tuberculosis, Antitubercular Agents, Workload, Biology, Sensitivity and Specificity, Microbiology, Mycobacterium tuberculosis, Young Adult, chemistry.chemical_compound, Tuberculosis diagnosis, Drug Resistance, Multiple, Bacterial, Virology, medicine, Humans, Centrifugation, Bacteriological Techniques, Tetrazolium chloride, Sputum, Articles, Middle Aged, medicine.disease, biology.organism_classification, Microscopic observation, Infectious Diseases, chemistry, Workforce, Colony count, Colorimetry, Female, Parasitology, medicine.symptom, Laboratories, Filtration
Abstract

Optimal tuberculosis testing usually involves sputum centrifugation followed by broth culture. However, centrifuges are biohazardous and scarce in the resource-limited settings where most tuberculosis occurs. To optimize tuberculosis testing for these settings, centrifugation of 111 decontaminated sputum samples was compared with syringe-aspiration through polycarbonate membrane-filters that were then cultured in broth. To reduce the workload of repeated microscopic screening of broth cultures for tuberculosis growth, the colorimetric redox indicator 2,3-diphenyl-5-(2-thienyl) tetrazolium chloride was added to the broth, which enabled naked-eye detection of culture positivity. This combination of filtration and colorimetric growth-detection gave similar results to sputum centrifugation followed by culture microscopy regarding mean colony counts (43 versus 48; P = 0.6), contamination rates (0.9% versus 1.8%; P = 0.3), and sensitivity (94% versus 95%; P = 0.7), suggesting equivalency of the two methods. By obviating centrifugation and repeated microscopic screening of cultures, this approach may constitute a more appropriate technology for rapid and sensitive tuberculosis diagnosis in basic laboratories.

Published
2010

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