1. Lifeact: a versatile marker to visualize F-actin
- Author
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Dieter E. Jenne, Alvaro H. Crevenna, Roland Wedlich-Söldner, Julia Riedl, Frank Bradke, Kai Kessenbrock, Michael Sixt, Michal Bista, Jerry Haochen Yu, Dorothee Neukirchen, Zena Werb, and Tad A. Holak
- Subjects
Recombinant Fusion Proteins ,Green Fluorescent Proteins ,Saccharomyces cerevisiae ,macromolecular substances ,Biology ,Biochemistry ,Filamentous actin ,Article ,Mice ,Live cell imaging ,In vivo ,Animals ,Humans ,Coloring Agents ,Cytoskeleton ,Molecular Biology ,Cells, Cultured ,Actin ,Staining and Labeling ,Cell Biology ,biology.organism_classification ,Actin cytoskeleton ,Actins ,In vitro ,Rats ,Cell biology ,Microscopy, Fluorescence ,Peptides ,Biotechnology - Abstract
Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.
- Published
- 2008
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