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801 results on '"Jerome, WG"'

1. Size- and charge-dependent non-specific uptake of PEGylated nanoparticles by macrophages

Author

Dickerson JH, Maron DJ, Jerome WG, Thomas SN, Lau CM, Yu SS, Hubbell JA, and Giorgio TD

Subjects
Medicine (General), R5-920
Abstract

Shann S Yu1,2, Cheryl M Lau1, Susan N Thomas3, W Gray Jerome4, David J Maron5, James H Dickerson2,6, Jeffrey A Hubbell3, Todd D Giorgio1,2,7,81Department of Biomedical Engineering, Vanderbilt University, Nashville, 2Vanderbilt Institute of Nanoscale Science and Engineering, Nashville, TN, USA; 3Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland, 4Department of Pathology, Vanderbilt University Medical Center, Nashville, 5Vanderbilt Heart and Vascular Institute, Nashville, 6Department of Physics and Astronomy, Vanderbilt University, Nashville, 7Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, 8Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, USAAbstract: The assessment of macrophage response to nanoparticles is a central component in the evaluation of new nanoparticle designs for future in vivo application. This work investigates which feature, nanoparticle size or charge, is more predictive of non-specific uptake of nanoparticles by macrophages. This was investigated by synthesizing a library of polymer-coated iron oxide micelles, spanning a range of 30–100 nm in diameter and -23 mV to +9 mV, and measuring internalization into macrophages in vitro. Nanoparticle size and charge both contributed towards non-specific uptake, but within the ranges investigated, size appears to be a more dominant predictor of uptake. Based on these results, a protease-responsive nanoparticle was synthesized, displaying a matrix metalloproteinase-9 (MMP-9)-cleavable polymeric corona. These nanoparticles are able to respond to MMP-9 activity through the shedding of 10–20 nm of hydrodynamic diameter. This MMP-9-triggered decrease in nanoparticle size also led to up to a six-fold decrease in nanoparticle internalization by macrophages and is observable by T2-weighted magnetic resonance imaging. These findings guide the design of imaging or therapeutic nanoparticles for in vivo targeting of macrophage activity in pathologic states.Keywords: macrophage targeting, poly(ethylene glycol) (PEG), poly(propylene sulfide) (PPS), iron oxides, opsonization

Published
2012

2. Autoimmune Vasculitis in Mrl/Mp-Lpr/Lpr Mice: Orthochromatic Basophils Participate in the Development Of Delayed-Type Hypersensitivity Angiitis

Author

Jerome Wg, Tulli H, Taylor R, Carolyn F. Moyer, and Reinisch Cl

Subjects
Vasculitis, Pathology, medicine.medical_specialty, Systemic disease, Immunology, chemical and pharmacologic phenomena, Basophil, medicine.disease_cause, Autoimmune Diseases, Autoimmunity, Pathogenesis, Lesion, Mice, medicine, Animals, Immunology and Allergy, Hypersensitivity, Delayed, business.industry, medicine.disease, Lymphoproliferative Disorders, Basophils, medicine.anatomical_structure, Delayed hypersensitivity, medicine.symptom, business, Blood vessel
Abstract

The pathogenesis of autoimmune vasculitis is poorly understood. Understanding the immunologic mechanisms governing this disease requires precise identification of the cells which comprise the lesion. In this report, we have evaluated tissue sections from MRL/lpr mice from 16 to 45 weeks of age, representing all stages of clinical vasculitis. We demonstrate that basophil myelocytes participate in the evolution of the delayed-type hypersensitivity (DTH) response which initiates and perpetuates autoimmune vasculitis in these mice. These findings raise questions regarding the immunologic mechanisms by which basophils develop in this lesion and the interaction of basophils. VSMCs and lymphocytes in vasculitic angiodestruction.

Published
1992

3. Radiopaque deposits surrounding a contact small-caliber gunshot wound

Author

Jerome Wg, Patrick E. Lantz, and Jaworski Ja

Subjects
Adult, Male, Electron probe microanalysis, Materials science, Injury control, Accident prevention, Poison control, Forensic Medicine, medicine.disease, Pathology and Forensic Medicine, Radiography, Suicide, Lead, Particulate material, medicine, Microscopy, Electron, Scanning, Humans, Small caliber, Wounds, Gunshot, Gunshot wound, Biomedical engineering, Electron Probe Microanalysis, Skin
Abstract

Primer residue typically contains some combination of lead, antimony, or barium, whereas bullet residue from nonjacketed bullets is 70-100% lead. The amount of lead particles produced by low-velocity bullets (projectile residue) may be exceedingly high. Copper- or brass-coated bullets generate an admixture of lead and copper particles whereas jacketed and semijacketed bullets produce the least amount of lead particles and only a small amount of copper-containing residue. We recently studied a suicidal contact small-caliber gunshot wound with an unusual amount of bullet residue deposited on the epidermal margins and within the wound track. The entrance wound was encircled by radiopaque particulate material visible on the postmortem radiograph. Soft radiography delineated the material to the wound track and adjacent epidermis. Scanning electron microscopy with energy-dispersive analysis of x-rays identified the principle element as lead. Language: en

Published
1994

11. Pancreatic islet production of vascular endothelial growth factor--a is essential for islet vascularization, revascularization, and function.

Author

Brissova M, Shostak A, Shiota M, Wiebe PO, Poffenberger G, Kantz J, Chen Z, Carr C, Jerome WG, Chen J, Baldwin HS, Nicholson W, Bader DM, Jetton T, Gannon M, Powers AC, Brissova, Marcela, Shostak, Alena, Shiota, Masakazu, and Wiebe, Peter O

Abstract

To investigate molecular mechanisms controlling islet vascularization and revascularization after transplantation, we examined pancreatic expression of three families of angiogenic factors and their receptors in differentiating endocrine cells and adult islets. Using intravital lectin labeling, we demonstrated that development of islet microvasculature and establishment of islet blood flow occur concomitantly with islet morphogenesis. Our genetic data indicate that vascular endothelial growth factor (VEGF)-A is a major regulator of islet vascularization and revascularization of transplanted islets. In spite of normal pancreatic insulin content and beta-cell mass, mice with beta-cell-reduced VEGF-A expression had impaired glucose-stimulated insulin secretion. By vascular or diffusion delivery of beta-cell secretagogues to islets, we showed that reduced insulin output is not a result of beta-cell dysfunction but rather caused by vascular alterations in islets. Taken together, our data indicate that the microvasculature plays an integral role in islet function. Factors modulating VEGF-A expression may influence islet vascularity and, consequently, the amount of insulin delivered into the systemic circulation. [ABSTRACT FROM AUTHOR]

Published
2006
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