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17. Gallic acid induces osteoblast differentiation and alleviates inflammatory response through GPR35/GSK3β/β‐catenin signaling pathway in human periodontal ligament cells.

Author

Pham, Thi Hoa, Kim, Eun‐Nam, Trang, Nguyen Minh, and Jeong, Gil‐Saeng

Subjects
INFLAMMATION prevention, PERIODONTAL disease prevention, CELL differentiation, LIPOPOLYSACCHARIDES, ALKALINE phosphatase, STATISTICS, CYTOKINES, PHENOLS, BONE growth, STAINS & staining (Microscopy), CELL culture, CELL migration, PERIODONTITIS, ANTI-inflammatory agents, ANIMAL experimentation, WESTERN immunoblotting, MICROBIOLOGICAL assay, NUCLEAR proteins, ONE-way analysis of variance, OSTEOBLASTS, ANTIOXIDANTS, CELLULAR signal transduction, TREATMENT effectiveness, RATS, CELL survival, ENZYME-linked immunosorbent assay, DESCRIPTIVE statistics, MOLECULAR structure, POLYMERASE chain reaction, REACTIVE oxygen species, DATA analysis, CELL surface antigens, PERIODONTAL ligament, IMMUNODIAGNOSIS, PHARMACODYNAMICS
Abstract

Background and Objective: Gallic acid (GA) possesses various beneficial functions including antioxidant, anticancer, anti‐inflammatory as well as inhibiting osteoclastogeneis. However, effects on osteogenic differentiation, especially in human ligament periodontal (hPDL) cells, remain unclear. Thus, the aim of this study was to evaluate the function of GA on osteogenesis and anti‐inflammation in hPDL cells and to explore the involved underlying mechanism. Methods: Porphyromonas gingivalis lipopolysaccharide (Pg‐LPS) treatment was used as a model for periodontitis. ROS production was determined by H2DCFDA staining. Trans‐well and wound healing assays were performed for checking the migration effect of GA. Alizarin red and alkaline phosphatase activity (ALP) assays were performed to evaluate osteogenic differentiation. Osteogenesis and inflammatory‐related genes and proteins were measured by real‐time PCR and western blot. Results: Our results showed that GA‐treated hPDL cells had higher proliferation and migration effect. GA inhibited ROS production‐induced by Pg‐LPS. Besides, GA abolished Pg‐LPS‐induced inflammation cytokines (il‐6, il‐1β) and inflammasome targets (Caspase‐1, NLRP3). In addition, GA promoted ALP activity and mineralization in hPDL cells, lead to enhance osteoblast differentiation process. The effect of GA is related to G‐protein‐coupled receptor 35 (GPR35)/GSK3β/β‐catenin signaling pathway. Conclusion: GA attenuated Pg‐LPS‐induced inflammatory responses and periodontitis in hPDL cells. Taken together, GA may be targeted for therapeutic interventions in periodontal diseases. [ABSTRACT FROM AUTHOR]

Published
2024
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18. Ameliorative Effect of Ginsenoside Rg6 in Periodontal Tissue Inflammation and Recovering Damaged Alveolar Bone.

Author

Lee, Won-Jin, Kim, Eun-Nam, Trang, Nguyen Minh, Lee, Jee-Hyun, Cho, Soo-Hyun, Choi, Hui-Ji, Song, Gyu-Yong, and Jeong, Gil-Saeng

Subjects
GINSENOSIDES, ALVEOLAR process, PORPHYROMONAS gingivalis, SPRAGUE Dawley rats, PERIODONTAL ligament
Abstract

Periodontal disease is a chronic disease with a high prevalence, and in order to secure natural materials to prevent oral diseases, new materials that protect periodontal tissue from inflammation are being sought. Genes were identified using real-time quantitative polymerase chain reaction (RT-qPCR), and proteins were confirmed using Western blot. Dichlorodihydrofluorescein diacetate (DCF-DA) analysis was used, and the antibacterial effects were confirmed through Minimum Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) analysis. To confirm this effect in vivo, Sprague–Dawley rats, in which periodontitis was induced using ligation or Lipopolysaccharide of Porphyromonas gingivalis (PG-LPS), were used. In vitro experiments using human periodontal ligament (HPDL) cells stimulated with PG-LPS showed that Ginsenoside Rg6 (G-Rg6) had anti-inflammatory, antibacterial, antioxidant, and osteoblast differentiation properties. In vivo, G-Rg6 was effective in Sprague–Dawley rats in which periodontitis was induced using ligation or PG-LPS. Therefore, Ginsenoside Rg6 shows potential effectiveness in alleviating periodontitis. [ABSTRACT FROM AUTHOR]

Published
2024
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32. Citropten Ameliorates Osteoclastogenesis Related to MAPK and PLCγ/Ca2+Signaling Pathways through the Regulation of Amyloid Beta

Author

Trang, Nguyen Minh, Kim, Eun-Nam, Pham, Thi Hoa, and Jeong, Gil-Saeng

Abstract

Osteoporosis and Alzheimer’s disease are typical types of dementia in seniors, which share common risk factors. Previous studies have shown that citizens with osteoporosis are more likely than healthy individuals to be at risk of Alzheimer’s disease. Citropten, found in Citrus aurantifolia, has been reported to have several pharmacological activities; however, its antiosteoclastogenic activity remains unknown. Here, receptor activator nuclear factor κB ligand (RANKL)-induced osteoclast differentiation, formation, and function in the presence of amyloid beta (Aβ) were attenuated by citropten in the RAW 264.7 cell line. The expression of osteoclast specific genes and proteins indicated that citropten pretreatment lowers the MAPK and PLCγ/Ca2+signaling pathways. Molecular docking simulations revealed that citropten interacts with the active sites of proteins in the calcium signaling pathway, which have negative binding affinities. These findings indicate that, through Aβ regulation, the RANKL-induced osteoclast can be suppressed by citropten, suggesting that citropten is a potential candidate for treating osteoclastogenesis-related diseases.

Published
2023
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