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The lean blowoff characteristics of a premixed air-methane flame were investigated in a ducted combustor with a bluff body according to acoustic excitation. The blowoff equivalence ratio increases with the Reynolds number and changes depending on the extent of the recirculation zone. Using the relation between the Damköhler number and the Reynolds number, it was confirmed that the flow velocity at the downstream tip of the bluff body and the laminar flame speed are decisive blowoff factors. Although a periodic flame hole appeared far from the blowoff only with acoustic excitation, the blowoff observed by OH radical chemiluminescence occurred using a similar process regardless of the excitation. The recirculation zone collapses and the flame becomes small when it is close to the blowoff. Then, the flame is locally extinguished downstream from the bluff body and the recirculation zone completely collapses. Eventually, the unburned gas does not ignite and the flame is extinguished. The blowoff equivalence ratio rapidly increases at specific acoustic excitation frequencies. This was investigated using proper orthogonal decomposition analysis, the two-microphone method, and phase-lock particle imaging velocimetry measurement. Resonance occurs when the excitation frequency approaches the harmonic frequency of the combustor and it increases the velocity fluctuation in the combustor and the infiltration velocity of the unburned gas in the shear layer of the recirculation zone. Consequently, because the burning velocity must have a larger value corresponding to the enhanced mixture velocity for a sustained flame, the blowoff occurs at a higher equivalence ratio. [ABSTRACT FROM PUBLISHER]

We announce the complete genome of Escherichia coli strain PEC1003 belonging to ST7366, isolated from sewage water in Pakistan. This strain harbored a plasmid carrying various antibiotic resistance genes, including extended-spectrum β-lactamase (ESBL), bla CTX-M-15 , emphasizing the management and surveillance of ESBL-producing E. coli in the environment of developing countries., Competing Interests: The authors declare no conflict of interest.

We present the complete genome sequence of Escherichia coli strain PEC1009 isolated from avian feces in Pakistan. The strain belongs to sequence type (ST) 10, a high-risk clone. The strain possesses two plasmids, and various plasmid-borne antibiotic resistance genes and chromosome-borne virulence factor genes were identified in its genome., Competing Interests: The authors declare no conflict of interest.

The emergence and spread of multidrug-resistance (MDR) pathogenic Escherichia coli due to horizontal gene transfer of antibiotic resistance genes (ARGs) and virulence factors (VFs) is a global health concern, particularly in developing countries. While numerous studies have focused on major sequence types (STs), the implication of minor STs in ARG dissemination and their pathogenicity remains crucial. In this study, two E. coli strains (PEC1011 and PEC1012) were isolated from wild bird feces in Pakistan and identified as ST2178 based on their complete genome sequences. To understand this minor ST, 204 genome assemblies of ST2178 were comparatively analyzed with the isolates' genomes. The phylogenetic analyses revealed five subclades of ST2178. Subclade E strains were predominantly isolated from human specimens, whereas subclades A and B strains including strains PEC1011 and PEC1012, respectively, were frequently isolated from animal. Mobile genetic elements (MGEs) exhibited the positive correlation with ARGs but not with VFs in this ST. Plasmid-borne ARGs exhibited higher correlation with plasmid-borne MGEs, indicating the role of diverse mobile plasmid structures in ARG transmission. Subclade E exhibited diverse plasmid-borne ARG repertoires correlated with MGEs, marking it as a critical surveillance target. In the case of VFs, they exhibited phylogeny-dependent profiles. Strain PEC1012 harbored various plasmid-borne ARGs, which are similar with conserved ARG repertoires in subclade A. The presence of unique ARG insertion in pPEC1012 highlights the importance of subclade A in ARG dissemination. This study comprehensively elucidates the landscape of ST2178, identifying critical phylogenetic subclades and their characteristics in ARG and VF occurrence.