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735 results on '"Jehmlich, Nico"'

4. Identification of inulin-responsive bacteria in the gut microbiota via multi-modal activity-based sorting

Author

Riva, Alessandra, Rasoulimehrabani, Hamid, Cruz-Rubio, José Manuel, Schnorr, Stephanie L., von Baeckmann, Cornelia, Inan, Deniz, Nikolov, Georgi, Herbold, Craig W., Hausmann, Bela, Pjevac, Petra, Schintlmeister, Arno, Spittler, Andreas, Palatinszky, Márton, Kadunic, Aida, Hieger, Norbert, Del Favero, Giorgia, von Bergen, Martin, Jehmlich, Nico, Watzka, Margarete, Lee, Kang Soo, Wiesenbauer, Julia, Khadem, Sanaz, Viernstein, Helmut, Stocker, Roman, Wagner, Michael, Kaiser, Christina, Richter, Andreas, Kleitz, Freddy, and Berry, David

Published
2023
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8. M cell maturation and cDC activation determine the onset of adaptive immune priming in the neonatal Peyer’s patch

Author

Torow, Natalia, Li, Ronghui, Hitch, Thomas Charles Adrian, Mingels, Clemens, Al Bounny, Shahed, van Best, Niels, Stange, Eva-Lena, Simons, Britta, Maié, Tiago, Rüttger, Lennart, Gubbi, Narasimha Murthy Keshava Prasad, Abbott, Darryl Adelaide, Benabid, Adam, Gadermayr, Michael, Runge, Solveig, Treichel, Nicole, Merhof, Dorit, Rosshart, Stephan Patrick, Jehmlich, Nico, Hand, Timothy Wesley, von Bergen, Martin, Heymann, Felix, Pabst, Oliver, Clavel, Thomas, Tacke, Frank, Lelouard, Hugues, Costa, Ivan Gesteira, and Hornef, Mathias Walter

Published
2023
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12. Global ecological predictors of the soil priming effect.

Author

Hayes, Patrick, Hernández, Teresa, Hseu, Zeng-Yei, Jehmlich, Nico, Kirchmair, Martin, Lambers, Hans, Neuhauser, Sigrid, Peña-Ramírez, Víctor, Pérez, Cecilia, Reed, Sasha, Santos, Fernanda, Siebe, Christina, Sullivan, Benjamin, Trivedi, Pankaj, Vera, Alfonso, Williams, Mark, Luis Moreno, José, Delgado-Baquerizo, Manuel, Bastida, Felipe, García, Carlos, Fierer, Noah, Eldridge, David, Bowker, Matthew, Abades, Sebastián, Alfaro, Fernando, Asefaw Berhe, Asmeret, Cutler, Nick, Gallardo, Antonio, García-Velázquez, Laura, and Hart, Stephen

Abstract

Identifying the global drivers of soil priming is essential to understanding C cycling in terrestrial ecosystems. We conducted a survey of soils across 86 globally-distributed locations, spanning a wide range of climates, biotic communities, and soil conditions, and evaluated the apparent soil priming effect using 13C-glucose labeling. Here we show that the magnitude of the positive apparent priming effect (increase in CO2 release through accelerated microbial biomass turnover) was negatively associated with SOC content and microbial respiration rates. Our statistical modeling suggests that apparent priming effects tend to be negative in more mesic sites associated with higher SOC contents. In contrast, a single-input of labile C causes positive apparent priming effects in more arid locations with low SOC contents. Our results provide solid evidence that SOC content plays a critical role in regulating apparent priming effects, with important implications for the improvement of C cycling models under global change scenarios.

Published
2019

13. Global ecological predictors of the soil priming effect.

Author

Bastida, Felipe, García, Carlos, Fierer, Noah, Eldridge, David J, Bowker, Matthew A, Abades, Sebastián, Alfaro, Fernando D, Asefaw Berhe, Asmeret, Cutler, Nick A, Gallardo, Antonio, García-Velázquez, Laura, Hart, Stephen C, Hayes, Patrick E, Hernández, Teresa, Hseu, Zeng-Yei, Jehmlich, Nico, Kirchmair, Martin, Lambers, Hans, Neuhauser, Sigrid, Peña-Ramírez, Víctor M, Pérez, Cecilia A, Reed, Sasha C, Santos, Fernanda, Siebe, Christina, Sullivan, Benjamin W, Trivedi, Pankaj, Vera, Alfonso, Williams, Mark A, Luis Moreno, José, and Delgado-Baquerizo, Manuel

Abstract

Identifying the global drivers of soil priming is essential to understanding C cycling in terrestrial ecosystems. We conducted a survey of soils across 86 globally-distributed locations, spanning a wide range of climates, biotic communities, and soil conditions, and evaluated the apparent soil priming effect using 13C-glucose labeling. Here we show that the magnitude of the positive apparent priming effect (increase in CO2 release through accelerated microbial biomass turnover) was negatively associated with SOC content and microbial respiration rates. Our statistical modeling suggests that apparent priming effects tend to be negative in more mesic sites associated with higher SOC contents. In contrast, a single-input of labile C causes positive apparent priming effects in more arid locations with low SOC contents. Our results provide solid evidence that SOC content plays a critical role in regulating apparent priming effects, with important implications for the improvement of C cycling models under global change scenarios.

Published
2019

14. Expanding the Use of Spectral Libraries in Proteomics

Author

Deutsch, Eric W, Perez-Riverol, Yasset, Chalkley, Robert J, Wilhelm, Mathias, Tate, Stephen, Sachsenberg, Timo, Walzer, Mathias, Käll, Lukas, Delanghe, Bernard, Böcker, Sebastian, Schymanski, Emma L, Wilmes, Paul, Dorfer, Viktoria, Kuster, Bernhard, Volders, Pieter-Jan, Jehmlich, Nico, Vissers, Johannes PC, Wolan, Dennis W, Wang, Ana Y, Mendoza, Luis, Shofstahl, Jim, Dowsey, Andrew W, Griss, Johannes, Salek, Reza M, Neumann, Steffen, Binz, Pierre-Alain, Lam, Henry, Vizcaíno, Juan Antonio, Bandeira, Nuno, and Röst, Hannes

Subjects
Animals, Databases, Protein, Humans, Peptide Library, Proteomics, Tandem Mass Spectrometry, Workflow, mass spectrometry, spectral libraries, standards, formats, Dagstuhl Seminar, meeting report, Proteomics Standards Initiative, Chemical Sciences, Biological Sciences, Biochemistry & Molecular Biology
Abstract

The 2017 Dagstuhl Seminar on Computational Proteomics provided an opportunity for a broad discussion on the current state and future directions of the generation and use of peptide tandem mass spectrometry spectral libraries. Their use in proteomics is growing slowly, but there are multiple challenges in the field that must be addressed to further increase the adoption of spectral libraries and related techniques. The primary bottlenecks are the paucity of high quality and comprehensive libraries and the general difficulty of adopting spectral library searching into existing workflows. There are several existing spectral library formats, but none captures a satisfactory level of metadata; therefore, a logical next improvement is to design a more advanced, Proteomics Standards Initiative-approved spectral library format that can encode all of the desired metadata. The group discussed a series of metadata requirements organized into three designations of completeness or quality, tentatively dubbed bronze, silver, and gold. The metadata can be organized at four different levels of granularity: at the collection (library) level, at the individual entry (peptide ion) level, at the peak (fragment ion) level, and at the peak annotation level. Strategies for encoding mass modifications in a consistent manner and the requirement for encoding high-quality and commonly seen but as-yet-unidentified spectra were discussed. The group also discussed related topics, including strategies for comparing two spectra, techniques for generating representative spectra for a library, approaches for selection of optimal signature ions for targeted workflows, and issues surrounding the merging of two or more libraries into one. We present here a review of this field and the challenges that the community must address in order to accelerate the adoption of spectral libraries in routine analysis of proteomics datasets.

Published
2018

17. Functional changes of the gastric bypass microbiota reactivate thermogenic adipose tissue and systemic glucose control via intestinal FXR-TGR5 crosstalk in diet-induced obesity

Author

Münzker, Julia, Haase, Nadine, Till, Andreas, Sucher, Robert, Haange, Sven-Bastiaan, Nemetschke, Linda, Gnad, Thorsten, Jäger, Elisabeth, Chen, Jiesi, Riede, Sjaak J., Chakaroun, Rima, Massier, Lucas, Kovacs, Peter, Ost, Mario, Rolle-Kampczyk, Ulrike, Jehmlich, Nico, Weiner, Juliane, Heiker, John T., Klöting, Nora, Seeger, Gudrun, Morawski, Markus, Keitel, Verena, Pfeifer, Alexander, von Bergen, Martin, Heeren, Joerg, Krügel, Ute, and Fenske, Wiebke K.

Published
2022
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19. An in vitro model system for testing chemical effects on microbiome-immune interactions – examples with BPX and PFAS mixtures.

Author

Fischer, Florence, Pierzchalski, Arkadiusz, Riesbeck, Sarah, Aldehoff, Alix Sarah, Castaneda-Monsalve, Victor Alfonso, Haange, Sven-Bastiaan, Bergen, Martin von, Rolle-Kampczyk, Ulrike Elisabeth, Jehmlich, Nico, Zenclussen, Ana Claudia, and Herberth, Gunda

Subjects
VITAMIN B2, CHEMICAL testing, FLUOROALKYL compounds, CHEMICAL systems, MONONUCLEAR leukocytes, TEST systems
Abstract

Introduction: More than 350,000 chemicals make up the chemical universe that surrounds us every day. The impact of this vast array of compounds on our health is still poorly understood. Manufacturers are required to carry out toxicological studies, for example on the reproductive or nervous systems, before putting a new substance on the market. However, toxicological safety does not exclude effects resulting from chronic exposure to low doses or effects on other potentially affected organ systems. This is the case for the microbiomeimmune interaction, which is not yet included in any safety studies. Methods: A high-throughput in vitro model was used to elucidate the potential effects of environmental chemicals and chemical mixtures on microbiomeimmune interactions. Therefore, a simplified human intestinal microbiota (SIHUMIx) consisting of eight bacterial species was cultured in vitro in a bioreactor that partially mimics intestinal conditions. The bacteria were continuously exposed to mixtures of representative and widely distributed environmental chemicals, i.e. bisphenols (BPX) and/or per- and polyfluoroalkyl substances (PFAS) at concentrations of 22 μM and 4 μM, respectively. Furthermore, changes in the immunostimulatory potential of exposed microbes were investigated using a co-culture system with human peripheral blood mononuclear cells (PBMCs). Results: The exposure to BPX, PFAS or their mixture did not influence the community structure and the riboflavin production of SIHUMIx in vitro. However, it altered the potential of the consortium to stimulate human immune cells: in particular, activation of CD8+ MAIT cells was affected by the exposure to BPX- and PFAS mixtures-treated bacteria. Discussion: The present study provides a model to investigate how environmental chemicals can indirectly affect immune cells via exposed microbes. It contributes to the much-needed knowledge on the effects of EDCs on an organ system that has been little explored in this context, especially from the perspective of cumulative exposure. [ABSTRACT FROM AUTHOR]

Published
2024
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21. DSS treatment does not affect murine colonic microbiota in absence of the host

Author

Krause, Jannike Lea, primary, Engelmann, Beatrice, additional, Schaepe, Stephanie Serena, additional, Rolle-Kampczyk, Ulrike, additional, Jehmlich, Nico, additional, Chang, Hyun-Dong, additional, Slanina, Ulla, additional, Hoffman, Maximillian, additional, Lehmann, Jörg, additional, Zenclussen, Ana Claudia, additional, Herberth, Gunda, additional, von Bergen, Martin, additional, and Haange, Sven-Bastiaan, additional

Published
2024
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22. Protein-Based Stable Isotope Probing (Protein-SIP): Applications for Studying Aromatic Hydrocarbon Degradation in Microbial Communities

Author

Jehmlich, Nico, von Bergen, Martin, Timmis, Kenneth N., Editor-in-Chief, Boll, Matthias, Series Editor, Geiger, Otto, Series Editor, Goldfine, Howard, Series Editor, Krell, Tino, Series Editor, Lee, Sang Yup, Series Editor, McGenity, Terry J., Series Editor, Rojo, Fernando, Series Editor, Sousa, Diana Z., Series Editor, Stams, Alfons J. M., Series Editor, Steffan, Robert J., Series Editor, and Wilkes, Heinz, Series Editor

Published
2020
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24. Genomewide meta‐analysis identifies loci associated with IGF‐I and IGFBP‐3 levels with impact on age‐related traits

Author

Teumer, Alexander, Qi, Qibin, Nethander, Maria, Aschard, Hugues, Bandinelli, Stefania, Beekman, Marian, Berndt, Sonja I, Bidlingmaier, Martin, Broer, Linda, Group, CHARGE Longevity Working, Cappola, Anne, Ceda, Gian Paolo, Chanock, Stephen, Chen, Ming‐Huei, Chen, Tai C, Chen, Yii‐Der Ida, Chung, Jonathan, Del Greco Miglianico, Fabiola, Eriksson, Joel, Ferrucci, Luigi, Friedrich, Nele, Gnewuch, Carsten, Goodarzi, Mark O, Grarup, Niels, Guo, Tingwei, Hammer, Elke, Hayes, Richard B, Hicks, Andrew A, Hofman, Albert, Houwing‐Duistermaat, Jeanine J, Hu, Frank, Hunter, David J, Husemoen, Lise L, Isaacs, Aaron, Jacobs, Kevin B, Janssen, Joop AMJL, Jansson, John‐Olov, Jehmlich, Nico, Johnson, Simon, Juul, Anders, Karlsson, Magnus, Kilpelainen, Tuomas O, Kovacs, Peter, Kraft, Peter, Li, Chao, Linneberg, Allan, Liu, Yongmei, Loos, Ruth JF, Consortium, Body Composition Genetics, Lorentzon, Mattias, Lu, Yingchang, Maggio, Marcello, Magi, Reedik, Meigs, James, Mellström, Dan, Nauck, Matthias, Newman, Anne B, Pollak, Michael N, Pramstaller, Peter P, Prokopenko, Inga, Psaty, Bruce M, Reincke, Martin, Rimm, Eric B, Rotter, Jerome I, Pierre, Aude Saint, Schurmann, Claudia, Seshadri, Sudha, Sjögren, Klara, Slagboom, P Eline, Strickler, Howard D, Stumvoll, Michael, Suh, Yousin, Sun, Qi, Zhang, Cuilin, Svensson, Johan, Tanaka, Toshiko, Tare, Archana, Tönjes, Anke, Uh, Hae‐Won, van Duijn, Cornelia M, van Heemst, Diana, Vandenput, Liesbeth, Vasan, Ramachandran S, Völker, Uwe, Willems, Sara M, Ohlsson, Claes, Wallaschofski, Henri, and Kaplan, Robert C

Subjects
Biological Sciences, Biomedical and Clinical Sciences, Genetics, Clinical Research, Aging, 2.1 Biological and endogenous factors, Aetiology, Metabolic and endocrine, Cardiovascular, Adult, Female, Gene Expression Regulation, Genome-Wide Association Study, Humans, Insulin-Like Growth Factor Binding Protein 3, Insulin-Like Growth Factor I, Male, Metabolome, Quantitative Trait Loci, Quantitative Trait, Heritable, Regulatory Sequences, Nucleic Acid, aging, genomewide association study, growth hormone axis, IGF-I, IGFBP-3, longevity, CHARGE Longevity Working Group, Body Composition Genetics Consortium, Medical and Health Sciences, Developmental Biology, Biological sciences, Biomedical and clinical sciences
Abstract

The growth hormone/insulin-like growth factor (IGF) axis can be manipulated in animal models to promote longevity, and IGF-related proteins including IGF-I and IGF-binding protein-3 (IGFBP-3) have also been implicated in risk of human diseases including cardiovascular diseases, diabetes, and cancer. Through genomewide association study of up to 30 884 adults of European ancestry from 21 studies, we confirmed and extended the list of previously identified loci associated with circulating IGF-I and IGFBP-3 concentrations (IGF1, IGFBP3, GCKR, TNS3, GHSR, FOXO3, ASXL2, NUBP2/IGFALS, SORCS2, and CELSR2). Significant sex interactions, which were characterized by different genotype-phenotype associations between men and women, were found only for associations of IGFBP-3 concentrations with SNPs at the loci IGFBP3 and SORCS2. Analyses of SNPs, gene expression, and protein levels suggested that interplay between IGFBP3 and genes within the NUBP2 locus (IGFALS and HAGH) may affect circulating IGF-I and IGFBP-3 concentrations. The IGF-I-decreasing allele of SNP rs934073, which is an eQTL of ASXL2, was associated with lower adiposity and higher likelihood of survival beyond 90 years. The known longevity-associated variant rs2153960 (FOXO3) was observed to be a genomewide significant SNP for IGF-I concentrations. Bioinformatics analysis suggested enrichment of putative regulatory elements among these IGF-I- and IGFBP-3-associated loci, particularly of rs646776 at CELSR2. In conclusion, this study identified several loci associated with circulating IGF-I and IGFBP-3 concentrations and provides clues to the potential role of the IGF axis in mediating effects of known (FOXO3) and novel (ASXL2) longevity-associated loci.

Published
2016

28. Biotransformation of Two Pharmaceuticals by the Ammonia-Oxidizing Archaeon Nitrososphaera gargensis

Author

Men, Yujie, Han, Ping, Helbling, Damian E, Jehmlich, Nico, Herbold, Craig, Gulde, Rebekka, Onnis-Hayden, Annalisa, Gu, April Z, Johnson, David R, Wagner, Michael, and Fenner, Kathrin

Subjects
Ammonia, Archaea, Bacteria, Biotransformation, Molecular Sequence Data, Oxidation-Reduction, Pharmaceutical Preparations, Phylogeny, Proteomics, Soil Microbiology, Environmental Sciences
Abstract

The biotransformation of some micropollutants has previously been observed to be positively associated with ammonia oxidation activities and the transcript abundance of the archaeal ammonia monooxygenase gene (amoA) in nitrifying activated sludge. Given the increasing interest in and potential importance of ammonia-oxidizing archaea (AOA), we investigated the capabilities of an AOA pure culture, Nitrososphaera gargensis, to biotransform ten micropollutants belonging to three structurally similar groups (i.e., phenylureas, tertiary amides, and tertiary amines). N. gargensis was able to biotransform two of the tertiary amines, mianserin (MIA) and ranitidine (RAN), exhibiting similar compound specificity as two ammonia-oxidizing bacteria (AOB) strains that were tested for comparison. The same MIA and RAN biotransformation reactions were carried out by both the AOA and AOB strains. The major transformation product (TP) of MIA, α-oxo MIA was likely formed via a two-step oxidation reaction. The first hydroxylation step is typically catalyzed by monooxygenases. Three RAN TP candidates were identified from nontarget analysis. Their tentative structures and possible biotransformation pathways were proposed. The biotransformation of MIA and RAN only occurred when ammonia oxidation was active, suggesting cometabolic transformations. Consistently, a comparative proteomic analysis revealed no significant differential expression of any protein-encoding gene in N. gargensis grown on ammonium with MIA or RAN compared with standard cultivation on ammonium only. Taken together, this study provides first important insights regarding the roles played by AOA in micropollutant biotransformation.

Published
2016

30. Identification of inulin-responsive bacteria in the gut microbiota via multi-modal activity-based sorting

Author

Berry, David, primary, Riva, Alessandra, additional, Rasoulimehrabani, Hamid, additional, Cruz-Rubio, José Manuel, additional, Schnorr, Stephanie, additional, von Baeckmann, Cornelia, additional, Inan, Deniz, additional, Nikolov, Georgi, additional, Herbold, Craig, additional, Hausmann, Bela, additional, Pjevac, Petra, additional, Schintlmeister, Arno, additional, Spittler, Andreas, additional, Palatinszky, Marton, additional, Kadunic, Aida, additional, Hieger, Norbert, additional, Favero, Giorgia Del, additional, von Bergen, Martin, additional, Jehmlich, Nico, additional, Watzka, Margarete, additional, Lee, Kang Soo, additional, Wiesenbauer, Julia, additional, Khadem, Sanaz, additional, Viernstein, Helmut, additional, Stocker, Roman, additional, Wagner, Michael, additional, Kaiser, Christina, additional, Richter, Andreas, additional, and Kleitz, Freddy, additional

Published
2023
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31. Widespread soil bacterium that oxidizes atmospheric methane

Author

Tveit, Alexander T., Hestnes, Anne Grethe, Robinson, Serina L., Schintlmeister, Arno, Dedysh, Svetlana N., Jehmlich, Nico, von Bergen, Martin, Herbold, Craig, Wagner, Michael, Richter, Andreas, and Svenning, Mette M.

Published
2019

32. Critical Assessment of MetaProteome Investigation (CAMPI): a multi-laboratory comparison of established workflows

Author

Van Den Bossche, Tim, Kunath, Benoit J., Schallert, Kay, Schäpe, Stephanie S., Abraham, Paul E., Armengaud, Jean, Arntzen, Magnus Ø., Bassignani, Ariane, Benndorf, Dirk, Fuchs, Stephan, Giannone, Richard J., Griffin, Timothy J., Hagen, Live H., Halder, Rashi, Henry, Céline, Hettich, Robert L., Heyer, Robert, Jagtap, Pratik, Jehmlich, Nico, Jensen, Marlene, Juste, Catherine, Kleiner, Manuel, Langella, Olivier, Lehmann, Theresa, Leith, Emma, May, Patrick, Mesuere, Bart, Miotello, Guylaine, Peters, Samantha L., Pible, Olivier, Queiros, Pedro T., Reichl, Udo, Renard, Bernhard Y., Schiebenhoefer, Henning, Sczyrba, Alexander, Tanca, Alessandro, Trappe, Kathrin, Trezzi, Jean-Pierre, Uzzau, Sergio, Verschaffelt, Pieter, von Bergen, Martin, Wilmes, Paul, Wolf, Maximilian, Martens, Lennart, and Muth, Thilo

Published
2021
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33. The Metaproteomics Initiative: a coordinated approach for propelling the functional characterization of microbiomes

Author

Van Den Bossche, Tim, Arntzen, Magnus Ø., Becher, Dörte, Benndorf, Dirk, Eijsink, Vincent G. H., Henry, Céline, Jagtap, Pratik D., Jehmlich, Nico, Juste, Catherine, Kunath, Benoit J., Mesuere, Bart, Muth, Thilo, Pope, Phillip B., Seifert, Jana, Tanca, Alessandro, Uzzau, Sergio, Wilmes, Paul, Hettich, Robert L., and Armengaud, Jean

Published
2021
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39. Human gut microbiome and metabolite dynamics under simulated microgravity

Author

Ramos-Nascimento, Ana, primary, Grenga, Lucia, additional, Haange, Sven-Bastiaan, additional, Himmelmann, Alexandra, additional, Arndt, Franca Sabine, additional, Ly, Yen-Tran, additional, Miotello, Guylaine, additional, Pible, Olivier, additional, Jehmlich, Nico, additional, Engelmann, Beatrice, additional, von Bergen, Martin, additional, Mulder, Edwin, additional, Frings-Meuthen, Petra, additional, Hellweg, Christine Elisabeth, additional, Jordan, Jens, additional, Rolle-Kampczyk, Ulrike, additional, Armengaud, Jean, additional, and Moeller, Ralf, additional

Published
2023
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40. Artefactual source of 2-hydroxypyridine

Author

Wilmes, Paul, primary, Trezzi, Jean-Pierre, additional, Aho, Velma, additional, Jäger, Christian, additional, Schade, Sebastian, additional, Janzen, Annette, additional, Hickl, Oskar, additional, Kunath, Benoit, additional, Thomas, Mélanie, additional, Schmit, Kristopher, additional, Garcia, Pierre, additional, Sciortino, Alessia, additional, Martin-Gallausiaux, Camille, additional, Halder, Rashi, additional, Huarte, Oihane Uriarte, additional, Heurtaux, Tony, additional, Heins-Marroquin, Ursula, additional, Gomez-Giro, Gemma, additional, Weidenbach, Katrin, additional, Delacour, Léa, additional, Laczny, Cédric, additional, Novikova, Polina, additional, Ramiro-Garcia, Javier, additional, Singh, Randolph, additional, Andújar, Begoña Talavera, additional, Lebrun, Laura, additional, Daujeumont, Annegrait, additional, Habier, Janine, additional, Dong, Xiangyi, additional, Gavotto, Floriane, additional, Heintz-Buschart, Anna, additional, Schneider, Jochen, additional, Jehmlich, Nico, additional, von Bergen, Martin, additional, Schymanski, Emma, additional, Schmitz, Ruth, additional, Schwamborn, Jens, additional, Glaab, Enrico, additional, Linster, Carole, additional, Kitami, Toshimori, additional, Buttini, Manuel, additional, May, Patrick, additional, Trenkwalder, Claudia, additional, Oertel, Wolfgang, additional, and Mollenhauer, Brit, additional

Published
2023
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44. Growth of complete ammonia oxidizers on guanidine

Author

Palatinszky, Marton, Herbold, Craig W., Sedlacek, Christopher J., Pühringer, Dominic, Kitzinger, Katharina, Giguere, Andrew T., Wasmund, Kenneth, Nielsen, Per H., Dueholm, Morten K. D., Jehmlich, Nico, Gruseck, Richard, Legin, Anton, Kostan, Julius, Krasnici, Nesrete, Schreiner, Claudia, Palmetzhofer, Johanna, Hofmann, Thilo, Zumstein, Michael, Djinović-Carugo, Kristina, Daims, Holger, and Wagner, Michael

Abstract

Guanidine is a chemically stable nitrogen compound that is excreted in human urine and is widely used in manufacturing of plastics, as a flame retardant and as a component of propellants, and is well known as a protein denaturant in biochemistry1–3. Guanidine occurs widely in nature and is used by several microorganisms as a nitrogen source, but microorganisms growing on guanidine as the only substrate have not yet been identified. Here we show that the complete ammonia oxidizer (comammox) Nitrospira inopinataand probably most other comammox microorganisms can grow on guanidine as the sole source of energy, reductant and nitrogen. Proteomics, enzyme kinetics and the crystal structure of a N. inopinataguanidinase homologue demonstrated that it is a bona fide guanidinase. Incubation experiments with comammox-containing agricultural soil and wastewater treatment plant microbiomes suggested that guanidine serves as substrate for nitrification in the environment. The identification of guanidine as a growth substrate for comammox shows an unexpected niche of these globally important nitrifiers and offers opportunities for their isolation.

Published
2024
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