78 results on '"Jebali J"'
Search Results
2. Metallothionein and metal levels in liver, gills and kidney of Sparus aurata exposed to sublethal doses of cadmium and copper
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Ghedira, J., Jebali, J., Bouraoui, Z., Banni, M., Guerbej, H., and Boussetta, H.
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- 2010
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3. Effects of malathion and cadmium on acetylcholinesterase activity and metallothionein levels in the fish Seriola dumerilli
- Author
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Jebali, J., Banni, M., Guerbej, H., Almeida, E. A., Bannaoui, A., and Boussetta, H.
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- 2006
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4. 24. Purification, biochemical and molecular characterization of an L-amino acid oxidase from Cerastes cerastes snake venom
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Abdelkafi-Koubaa, Z., primary, Jebali, J., additional, Morjen, M., additional, Othman, H., additional, Zouari-Kesentini, R., additional, Aissa, I., additional, Bazaa, A., additional, Majdoub, H., additional, Srairi-Abid, N., additional, Gargouri, Y., additional, El Ayeb, M., additional, and Marrakchi, N., additional
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- 2014
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5. دراسة لكمية ﻭﻨﻭﻋﯿﺔ ' المتلوتيونيين' المركزة في مختلف أعضاء سمك ﺃﻠﻭﺮﻗﺔ Sparus aurata اثر تعرضها للملوثات المعدنية ، اﻠﻛﺎدميوم و النحاس
- Author
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Ameur, S., Ghedira, J., Guerbej, H., Jebali, J., Bouraoui, J., and Boussetta, H.
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Gills ,Pollutants ,Animal organs ,Pollution effects ,Metallic elements ,Biomarkers of exposure (organisms) ,Bioaccumulation ,Heavy metals ,Trace metals ,Metals ,Sediment pollution ,Toxicity tests ,Marine sciences ,Cadmium - Abstract
Dans l’environnement marin, les éléments traces peuvent s’accumuler dans les organismes à des concentrations supérieures à celles présentes dans l’eau. L’étude de la distribution tissulaire des métaux traces au niveau des organismes qui y sont exposés, constitue un aspect très important pour la compréhension de leur devenir. En outre, le suivi de la réponse biologique spécifique de ces organismes sentinelles aux xénobiotiques complète et consolide l’analyse chimique. Ce travail vise à étudier la distribution tissulaire des métaux traces et des métallothionéines (MTs) chez la daurade «Sparus aurata » traitée par un métal essentiel (le cuivre) et un métal non essentiel (le cadmium) à une concentration de 500µg /Kg de poids frais pendant 48 heures. L’exposition de S. aurata aux deux métaux provoque une accumulation différentielle selon l’organe avec un maximum de bioaccumulation au niveau du foie. L’induction des MTs est significative au niveau des branchies et des reins avec une teneur maximal au niveau du foie. La plus haute teneur des MTs observée au niveau du foie est corrélée aux fortes concentrations de Cu et de Cd. La forte induction des MTs au niveau de différents organes des poissons traités confirme les rôles des MTs dans l’homéostasie et la détoxification des métaux lourds de l’organisme. Par spectroscopie d’impédance nous a montré que le cuivre a un pouvoir inducteur des MTs plus puissant que le cadmium. Mots clés : métaux lourds, Sparus aurata, métallothionéines, spectrophotométrie d’absorption atomique, للمعادن الثقيلة انعكاس بيئي سلبي و ذو خطورة عالية على المنظومة البحرية و صحة اﻠﻛﺎﺌﻨﺎﺖ و من بين ها ته المعادن التي يقع إﻠﻗﺎؤھﺎ في البحر نجد اﻠﻛﺎدميوم، النحاس، الزئبق و الرصاص. تلعب " المتلوتيونيين" دورا هاما في إزالة التسمم بالتصدي لها ته المعادن في الجسم. فى هذا العمل، وقع دراسة " المتلوتيونيين" في ﺒﻌﺾ أعضاء سمك ﺍﻠﻭﺮﻗﺔ Sparus aurata اثر تعرضها في المخبر للملوثات المعدنية، اﻠﻛﺎدميوم و النحاس بمعدلﻤﯿﻛﺮﻭﻏﺮﺍﻢ/ﻛﯿﻠﻭﻏﺮﺍﻢ 500 لمدة يومين. لقد أثبتت الدراسة ارتفاع مستوى الملوثات المعدنية، اﻠﻛﺎدميوم و النحاس في مختلف أعضاء السمكS. aurata كما لاحظنا ارتفاعا عال لمستوى الملوثات داخل الكبد. ﻠلتخلص من ها ته الملوثات, تفرز السمكة كميات هائلة من " المتلوتيونيين" في مختلف أعضائها. و تأكيدا لهذه الملاحظة فقد سجلنا إفرازا مرتفعا لمستوى الملوثات داخل الكبد تماشيا مع المستوى للملوثات داخل هدا العضو. Marine ecosystem is threatened by increasing levels of various pollutants originating from human activities, urban, agricultural and industrial discharges. Such situation endangers health of organisms inhabiting marine ecosystem. Among anthropogenic contaminants, heavy metals were widely detected in sediments at different orders of magnitude. Investigation heavy metals organotropism for exposed organisms is a very important since let to comprehension of pollutant behaviour (Geret, 2000). Levels of metallothionein (MTs), (biomarker of metal exposure) were determined in Sparus aurata intraperitoneally injected with 500µg/Kg of Cu and Cd for 2 days. MTs levels and metal concentrations (Cd and Cu), were determined in liver, gills and kidney. MTs levels increased significantly in all tissues with highest level in liver with (3.56 fold and 3.39 fold of Cu and Cd respectively). Metal concentrations were significantly different between investigated tissues. Highest Cd and Cu concentrations were observed in liver. Higher MTs induction levels in different tissues of treated fishes support the main role of MTs in metal homeostasis and detoxification. Else MTs analysis by spectroscopic of impedance showed that copper was stronger MTs inducing than the cadmium. Published Sparus aurata
- Published
- 2009
6. Use of oxidative stress biomarkers in Carcinus maenas to assess littoral zone contamination in Tunisia
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Ghedira, J, primary, Jebali, J, additional, Banni, M, additional, Chouba, L, additional, Boussetta, H, additional, López-Barea, J, additional, and Alhama, J, additional
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- 2011
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7. Characterization and evaluation of cholinesterase activity in the cockle Cerastoderma glaucum
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Jebali, J, primary, Ben-Khedher, S, additional, Kamel, N, additional, Ghedira, J, additional, Bouraoui, Z, additional, and Boussetta, H, additional
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- 2011
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8. Acute effects of benzo[a]pyrene on digestive gland enzymatic biomarkers and DNA damage on mussel Mytilus galloprovincialis
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Banni, M., primary, Negri, A., additional, Dagnino, A., additional, Jebali, J., additional, Ameur, S., additional, and Boussetta, H., additional
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- 2010
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9. Metallothionein and metal levels in liver, gills and kidney of Sparus aurata exposed to sublethal doses of cadmium and copper
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Ghedira, J., primary, Jebali, J., additional, Bouraoui, Z., additional, Banni, M., additional, Guerbej, H., additional, and Boussetta, H., additional
- Published
- 2008
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10. Assessment of heavy metal contamination using real-time PCR analysis of mussel metallothioneinmt10andmt20expression: a validation along the Tunisian coast
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Banni, M., primary, Dondero, F., additional, Jebali, J., additional, Guerbej, H., additional, Boussetta, H., additional, and Viarengo, A., additional
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- 2007
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11. Monitoring pollution in Tunisian coasts: application of a classification scale based on biochemical markers
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Banni, M., primary, Jebali, J., additional, Daubeze, M., additional, Clerandau, C., additional, Guerbej, H., additional, Narbonne, J. F., additional, and Boussetta, H., additional
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- 2005
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12. Assessment of heavy metal contamination using real-time PCR analysis of mussel metallothionein mt10 and mt20 expression: a validation along the Tunisian coast.
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Banni, M., Dondero, F., Jebali, J., Guerbej, H., Boussetta, H., and Viarengo, A.
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METALS ,MUSSELS ,TISSUES ,METALLOTHIONEIN ,MESSENGER RNA ,POLYMERASE chain reaction - Abstract
In mussel Mytilus galloprovincialis tissues, metallothionein belongs to two different gene classes, mt10 and mt20, showing differential expression at both basal conditions and under heavy metal challenge. In this study, a new more highly sensitive technique, expression analysis of mt10 and mt20 mRNA levels by quantitative reverse transcription polymerase chain reaction, was used to assess the effects of heavy metal contamination in the digestive glands of mussels caged along the Tunisian coast. To validate the new assay, total metallothionein protein, amount of heavy metals (zinc, copper, cadmium), and a biomarker of oxidative stress such as malondialdehyde content, were assessed in the same tissues. At the investigated sites, the molecular assay showed variations of mt20 relative gene expression levels within one or two orders of magnitude, with maximum values at two sites severely polluted with cadmium, Mahres (100-fold) and Menzel Jemile (165-fold). Changes in mt10 expression were recorded at all sites where copper had significantly accumulated, although fold induction levels were less pronounced than those of mt20. In this paper, gene expression data are discussed in relation to the studied biomarkers, demonstrating that the molecular technique based on the differential expression of mt10 and mt20 genes represents (i) a useful and robust tool for studying and monitoring heavy metal pollution under field conditions, and (ii) an improvement in the application of metallothionein as a biomarker of response to exposure to heavy metals in marine mussels. [ABSTRACT FROM AUTHOR]
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- 2007
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13. Effects of cadmium and benzo[a]pyrene on phase I and phase II detoxification on the polychaete Nereis diversicolor | Étude de l'effet du cadmium et du benzo[a]pyrène sur des enzymes de phase I at phase II de biotransformation chez le polychète Nereis diversicolor
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Bouraoui, Z., Ghedira, J., Jebali, J., Mohamed Banni, Clerendeau, C., Narbonne, J. -F, and Boussetta, H.
14. Chemical characterization and pharmacological properties of polysaccharides from Allium roseum leaves: In vitro and in vivo assays.
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Kherroubi S, Morjen M, Teka N, Mraihi F, Srairi-Abid N, Le Cerf D, Marrakchi N, Majdoub H, Cherif JK, Jebali J, and Ternane R
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- Animals, Humans, Rats, Plant Extracts pharmacology, Plant Extracts chemistry, Male, Edema drug therapy, Edema chemically induced, Macrophages drug effects, Macrophages metabolism, THP-1 Cells, Polysaccharides pharmacology, Polysaccharides chemistry, Polysaccharides isolation & purification, Plant Leaves chemistry, Antioxidants pharmacology, Antioxidants chemistry, Anti-Inflammatory Agents pharmacology, Anti-Inflammatory Agents chemistry, Allium chemistry
- Abstract
Allium roseum is amongst the most important wild medicinal plants. It is known for its diverse biological properties, including antioxidant, antibacterial and antidiabetic activities. In this work, the polysaccharides (PARLs) were ultrasonically extracted from Allium roesum leaves then purified and analyzed by several techniques. Chemical composition and GC-MS analysis showed that the obtained polysaccharides were composed mainly of glucose (40.20 %), mannose (25.30 %), fructose (10.60 %) and galacturonic acid (15.11 %). Moreover, PARLs exhibited a potent antioxidant effect with higher capacities up to 69.61 % and 71.72 % for DPPH and ABTS free radicals, respectively. Furthermore, PARLs significantly modulated inflammatory response by reducing TNF-α, IL-6, and IL-8 pro-inflammatory mediators and promoting the anti-inflammatory IL-10 mediator in LPS stimulated THP-1 derived macrophages. The in-vivo tests proved that the extract was able to decrease carrageenan-induced rat paw swelling by around 68.15 % after 4 h of treatment. PARLs, significantly reduced the growth of U87 (glioblastoma) and IGROV-1 cancer cells with IC
50 values of about 4.27 and 7.89 mg/mL respectively. This research clearly shows that Allium roseum polysaccharides can be used as natural antioxidants with anti-inflammatory and anticancer properties., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
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15. Anticancer Properties of Different Varieties of Date Palm (Phoenix dactylifera L.) Leaf Extracts in Human Tumor Cells: a Comparative Study.
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Chakroun M, Morjen M, Mabrouk HB, Mejdoub H, Srairi-Abid N, Marrakchi N, Jebali J, and Khemakhem B
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- Humans, Cell Line, Tumor, Breast Neoplasms drug therapy, Tunisia, Polyphenols pharmacology, Polyphenols analysis, Phoeniceae chemistry, Plant Extracts pharmacology, Plant Leaves chemistry, Glioblastoma drug therapy, Antineoplastic Agents, Phytogenic pharmacology, Cell Adhesion drug effects, Cell Movement drug effects
- Abstract
Plant polyphenols are nutraceutical components with relevant biological effects on human health. They act against development of several diseases including cancer. In this study, the methanolic extracts of four date palm Phoenix dactylifera leaves (Deglet Noor (DN), Barhee (B), Khalas (KS) and Khunezi (KZ)) collected from south Tunisia were preliminary analyzed for their effects against U87 (human glioblastoma) and MDA-MB-231 (human breast cancer) cell line development. Results showed that Barhee extract (30 μg/mL) was the most efficient to reduce the growth of both tumor cells to about 40% (p < 0.05) without inducing cytotoxicity. Significantly, KS, KZ, DN and B extracts (30 μg/mL) decreased MDA-MB-231 and U87 cell adhesion towards fibrinogen and fibronectin. Using integrin blocking antibodies, leaf extracts competitively decreased human glioblastoma cell attachment to immobilized antibodies by interfering to αvβ3 and α5β1 integrin receptors. At the same concentration, extracts decreased MDA-MB-23 and U87 cell migration performed with wound healing assay. Particularly, Barhee and Deglet Noor leaf extracts (30 μg/mL) significantly reduced U87 cell invasion by 52.92% (p < 0.01) and 74.56% (p < 0.01), respectively. Collegially, our findings revealed beneficial proprieties of four varieties of date palm leaf especially those displayed by DN and B extracts that may serve as active candidates against human glioblastoma and breast cancer progression., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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- 2024
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16. Biphasic Hormetic-like Effect of Lebecetin, a C-type Lectin of Snake Venom, on Formalin-induced Inflammation in Mice.
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Belardo C, Jebali J, Boccella S, Infantino R, Fusco A, Perrone M, Bonsale R, Manzo I, Iannotta M, Scuteri D, Ferraraccio F, Panarese I, Ferrara G, Guida F, Luongo L, Palazzo E, Srairi-Abid N, Marrakchi N, and Maione S
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- Animals, Male, Mice, Cyclooxygenase 2 metabolism, Cytokines metabolism, Edema drug therapy, Edema chemically induced, Nitric Oxide Synthase Type II metabolism, Pain drug therapy, Pain chemically induced, Spinal Cord drug effects, Spinal Cord metabolism, Viper Venoms pharmacology, Viper Venoms therapeutic use, Formaldehyde, Inflammation chemically induced, Inflammation drug therapy, Lectins, C-Type therapeutic use
- Abstract
Background: Integrins, important extracellular matrix (ECM) receptor proteins, are affected by inflammation and can participate in the maintenance of many painful conditions. Although they are ubiquitous and changeable across all cell types, the roles of these cell adhesion molecules in pathological pain have not been fully explored., Objective: We evaluated the effects of the subcutaneous injection of lebecetin, a C-type lectin isolated from Macrovipera lebetina snake venom, previously reported to inhibit α5β1 and αv integrin activity, on different components of inflammation induced by the formalin administration in the hind paw of mice., Methods: The formalin-induced nocifensive behavior, edema, and histopathological changes in the hind paw associated with cytokine, iNOS, and COX2 expression, nociceptive-specific neuron activity, and microglial activation analysis in the spinal cord were evaluated in mice receiving vehicle or lebecetin pretreatment., Results: Lebecetin inhibited the nocifensive responses in the formalin test, related edema, and cell infiltration in the injected paw in a biphasic, hormetic-like, and dose-dependent way. According to that hormetic trend, a reduction in pro-inflammatory cytokines IL-6, IL-8, and TNF-alpha and upregulation of the anti-inflammatory cytokine IL-10 in the spinal cord were found with the lowest doses of lebecetin. Moreover, COX2 and iNOS expression in serum and spinal cord followed the same biphasic pattern of cytokines. Finally, nociceptive neurons sensitization and activated microglia were normalized in the dorsal horn of the spinal cord by lebecetin., Conclusion: These findings implicate specific roles of integrins in inflammation and tonic pain, as well as in the related central nervous system sequelae., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
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- 2024
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17. CC5 and CC8, Two Disintegrin Isoforms from Cerastes cerastes Snake Venom Decreased Inflammation Response In Vitro and In Vivo.
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Morjen M, Zakraoui O, Abdelkafi-Koubaa Z, Srairi-Abid N, Marrakchi N, Essafi-Benkhadir K, and Jebali J
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- Rats, Mice, Humans, Animals, Lipopolysaccharides toxicity, Snake Venoms pharmacology, NF-kappa B metabolism, Inflammation drug therapy, Cytokines metabolism, Protein Isoforms, Anti-Inflammatory Agents pharmacology, Anti-Inflammatory Agents therapeutic use, RAW 264.7 Cells, Disintegrins pharmacology, Viperidae metabolism
- Abstract
Inflammation is associated with many pathology disorders and the malignant progression of most cancers. Therefore, targeting inflammatory pathways could provide a promising strategy for disease prevention and treatment. In this study, we experimentally investigated the anti-inflammatory effect of CC5 and CC8, two disintegrin isoforms isolated from Cerastes cerastes snake venom, on LPS-stimulated macrophages, both on human THP-1 and mouse RAW264.7 cell adherence and their underlying mechanisms by measuring cytokine release levels and Western blot assay. Equally, both molecules were evaluated on a carrageenan-induced edema rat model. Our findings suggest that CC5 and CC8 were able to reduce adhesion of LPS-stimulated macrophages both on human THP-1 and mouse RAW264.7 cells to fibrinogen and vitronectin through the interaction with the αvβ3 integrin receptor. Moreover, CC5 and CC8 reduced the levels of reactive oxygen species (ROS) mediated by the NF-κB, MAPK and AKT signaling pathways that lead to decreased production of the pro-inflammatory cytokines TNF-α, IL-6 and IL-8 and increased secretion of IL-10 in LPS-stimulated THP-1 and RAW264.7 cells. Interestingly, both molecules potently exhibited an anti-inflammatory effect in vivo by reducing paw swelling in rats. In light of these results, we can propose the CC5 and CC8 disintegrins as interesting tools to design potential candidates against inflammatory-related diseases.
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- 2023
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18. Insights into the mechanisms governing P01 scorpion toxin effect against U87 glioblastoma cells oncogenesis.
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Mlayah-Bellalouna S, Aissaoui-Zid D, Chantome A, Jebali J, Souid S, Ayedi E, Mejdoub H, Belghazi M, Marrakchi N, Essafi-Benkhadir K, Vandier C, and Srairi-Abid N
- Abstract
The emerging concept of small conductance Ca
2+ -activated potassium channels (SKCa ) as pharmacological target for cancer treatment has significantly increased in recent years. In this study, we isolated the P01 toxin from Androctonus australis (Aa) scorpion venom and investigated its effect on biological properties of glioblastoma U87, breast MDA-MB231 and colon adenocarcinoma LS174 cancer cell lines. Our results showed that P01 was active only on U87 glioblastoma cells. It inhibited their proliferation, adhesion and migration with IC50 values in the micromolar range. We have also shown that P01 reduced the amplitude of the currents recorded in HEK293 cells expressing SK2 channels with an IC50 value of 3 pM, while it had no effect on those expressing SK3 channels. The investigation of the SKCa channels expression pattern showed that SK2 transcripts were expressed differently in the three cancer cell lines. Particularly, we highlighted the presence of SK2 isoforms in U87 cells, which could explain and rely on the specific activity of P01 on this cell line. These experimental data highlighted the usefulness of scorpion peptides to decipher the role of SKCa channels in the tumorigenesis process, and develop potential therapeutic molecules targeting glioblastoma with high selectivity., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Mlayah-Bellalouna, Aissaoui-Zid, Chantome, Jebali, Souid, Ayedi, Mejdoub, Belghazi, Marrakchi, Essafi-Benkhadir, Vandier and Srairi-Abid.)- Published
- 2023
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19. Assessment of single and combined effects of bisphenol-A and its analogue bisphenol-S on biochemical and histopathological responses of sea cucumber Holothuria poli.
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Jenzri M, Gharred C, Bouraoui Z, Guerbej H, Jebali J, and Gharred T
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- Animals, Acetylcholinesterase, Ecosystem, Biomarkers, Holothuria, Sea Cucumbers
- Abstract
Bisphenols (BPs) are among emerging pollutants that have been frequently detected in different compartments of marine ecosystems and elicited great concern due to their potential toxicity to marine organisms. This work aimed to investigate the toxicity of bisphenol A (BPA) and bisphenol S (BPS) on oxidative stress markers, neurotoxicity and histopathological alterations in sea cucumbers (Holothuria poli). The results showed that exposure to 200 μg/L of BPA and BPS produced oxidative stress, neurotoxicity in the digestive tract and respiratory tree, and several types of histopathological lesions in tissues of the respiratory tree of the sea cucumber, posing a health hazard to this aquatic organism. In addition, BPA has greater effects than BPS on the generation of oxidative stress marked by the inductions of catalase (CAT), glutathione S-transferase (GST) and malondialdehyde (MDA) levels and neurotoxicity shown by the decrease in acetylcholinesterase activity (AChE). The respiratory tree of sea cucumbers might be an appropriate tissue for assessing CAT, MDA and AChE activity levels, which are reliable biomarkers that may be useful in marine biomonitoring studies. Evaluation of histopathological lesions of the respiratory tree suggests that BPA and BPS and their mixture cause various tissue alterations that may be associated with oxidative stress damage and neurotoxicity. In conclusion, this study showed that oxidative stress (CAT and MDA) and neurotoxicity (AChE) markers, as well as respiratory tree lesions, are sensitive biomarkers for the assessment of BPA and BPS toxicity in sea cucumbers., Competing Interests: Declaration of competing interest All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest or nonfinancial interest in the subject matter or materials discussed in this manuscript., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2023
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20. PpSP32, the Phlebotomus papatasi immunodominant salivary protein, exerts immunomodulatory effects on human monocytes, macrophages, and lymphocytes.
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Souissi C, Marzouki S, Elbini-Dhouib I, Jebali J, Oliveira F, Valenzuela JG, Srairi-Abid N, Kamhawi S, and Ben Ahmed M
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- Humans, Rats, Animals, Monocytes, NF-kappa B, Carrageenan, Lipopolysaccharides, Lymphocytes, Macrophages, Cytokines, Salivary Proteins and Peptides, Phlebotomus parasitology
- Abstract
Background: The saliva of sand flies, vectors of Leishmania parasites, contains several components that exert pharmacological activity facilitating the acquisition of blood by the insect and contributing to the establishment of infection. Previously, we demonstrated that PpSP32 is the immunodominant salivary antigen in humans exposed to Phlebotomus papatasi bites and validated its usefulness as a predictive biomarker of disease. PpSP32, whose functions are little known to date, is an intriguing protein due to its involvement in the etiopathogenesis of pemphigus, an auto-immune disease. Herein, we aimed to better decipher its role through the screening of several immunomodulatory activity either on lymphocytes or on monocytes/macrophages., Methods: Peripheral mononuclear cells from healthy volunteers were stimulated with anti-CD3/anti-CD28 antibodies, phytohemagglutinin, phorbol 12-myristate 13-acetate/ionomycin, or lipopolysaccharide in the presence of increasing doses of PpSP32. Cell proliferation was measured after the addition of tritiated thymidine. Monocyte activation was tested by analyzing the expression of CD86 and HLA-DR molecules by flow cytometry. Cytokine production was analyzed in culture supernatants by ELISA. THP-1-derived macrophages were stimulated with LPS in the presence of increasing doses of PpSP32, and cytokine production was analyzed in culture supernatants by ELISA and multiplex technique. The effect of PpSP32 on NF-kB signaling was tested by Western blot. The anti-inflammatory activity of PpSP32 was assessed in vivo in an experimental inflammatory model of carrageenan-induced paw edema in rats., Results: Our data showed that PpSP32 down-modulated the expression of activation markers in LPS-stimulated monocytes and THP1-derived macrophages. This protein negatively modulated the secretion of Th1 and Th2 cytokines by human lymphocytes as well as pro-inflammatory cytokines by monocytes, and THP1-derived macrophages. PpSP32 treatment led to a dose-dependent reduction of IκB phosphorylation. When PpSP32 was injected into the paw of carrageenan-injected rats, edema was significantly reduced., Conclusions: Our data indicates that PpSP32 induces a potent immunomodulatory effect on monocytes and THP-1-derived macrophages. This inhibition could be mediated, among others, by the modulation of the NF-kB signaling pathway. The anti-inflammatory activity of PpSP32 was confirmed in vivo in the carrageenan-induced paw edema model in rats., (© 2023. The Author(s).)
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- 2023
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21. Does thermal stress modulate the biochemical and physiological responses of Ruditapes decussatus exposed to the progestin levonorgestrel?
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Mannai A, Hmida L, Bouraoui Z, Guerbej H, Gharred T, and Jebali J
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- Female, Animals, Progestins metabolism, Progestins pharmacology, Levonorgestrel pharmacology, Levonorgestrel metabolism, Oxidative Stress, Water Pollutants, Chemical analysis, Bivalvia
- Abstract
In this study, we investigated the effects of 1000 ng/l levonorgestrel (LNG) alone or combined with increased temperature of 20, 24, and 28 °C on the biochemical and physiological responses of the clam (Ruditapes decussatus) for 28 days. Our results revealed that female clams treated with levonorgestrel (LNG) alone showed enhancement of the antioxidant defense against oxidative stress related to the inductions of catalase (CAT), gluthatione -S -transferase (GST), and protein sulfhydryl (PSH), while the elevated temperatures of 20, 24, and 28 °C diminished most of the specific responses to LNG and was the main factor in the determining the responses to combine exposures. The responses of lysosomal membrane stability, alkaline phosphatase, and NADP
+ -dependent isocitrate dehydrogenase detected were the most common signs of an adverse effect in all exposures. Female clams' testosterone and estradiol responses to LNG were the most particular manifestations depending on the exposure. Overall, these findings showed clearly that chronic warming stress caused disruption in physiological, biochemical parameters of the female clam R. decussatus, and this may have implications for the whole organism and populations., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)- Published
- 2022
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22. Investigation of the Renal Protective Effect of Combined Dietary Polyphenols in Streptozotocin-Induced Diabetic Aged Rats.
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Chtourou Y, Morjen M, Ammar R, Mhiri R, Jemaà M, ELBini-Dhouib I, Fetoui H, Srairi-Abid N, Marrakchi N, and Jebali J
- Subjects
- Animals, Antioxidants metabolism, Kidney metabolism, Oxidative Stress, Polyphenols therapeutic use, Rats, Rats, Wistar, Streptozocin, Transforming Growth Factor beta metabolism, Diabetes Mellitus, Experimental metabolism, Diabetic Nephropathies drug therapy, Diabetic Nephropathies metabolism, Diabetic Nephropathies prevention & control
- Abstract
Natural polyphenols are widely reported to have a large range of pharmacological properties, especially antioxidant activities and free radical scavenging capacities. In this study, we investigate the effects of naringin, chlorogenic acid, and quercetin mixtures (NCQ) on renal fibrosis in streptozotocin (STZ)-induced diabetic aged rats and its underlying mechanisms for ten consecutive weeks. The oxidative defense system in the kidneys of treated rats was found to be improved. Several biomarkers were investigated including the blood urea nitrogen, creatinine, and uric acid. Moreover, antioxidant parameters were evaluated and we found that superoxide dismutase, catalase, glutathione peroxidase, Na
+ -K+ -ATPase activities, the nitric oxide production, the protein carbonyl, the advanced oxidation protein products, lipid peroxidation, and reduced glutathione levels were all significantly balanced and close to control values. In addition, NCQ restored renal injuries and fibrosis as assessed by histological method and molecular biology investigation of the matrix metalloproteinase, the transforming growth factor-beta TGF-β, the tumor necrosis factor TNFα, and p53 expression. Our study proposes the NCQ combination as potential plant-derived bioactive compounds to prevent diabetic nephropathy.- Published
- 2022
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23. Expression of the First Recombinant Anti-Tumoral Snake Venom Kunitz-Type Serine Protease Inhibitor.
- Author
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Morjen M, Moslah W, Touihri-Baraketi I, Srairi-Abid N, Luis J, Marrakchi N, and Jebali J
- Subjects
- Humans, Serine, Serine Proteinase Inhibitors chemistry, Serine Proteinase Inhibitors genetics, Serine Proteinase Inhibitors pharmacology, Snake Venoms, Antivenins, Glioblastoma drug therapy
- Abstract
PIVL is a Kunitz-type serine protease inhibitor that was previously characterized from Tunisian snake venom, Macrovipera lebetina transmediterranea . It reduced glioblastoma cells' development and significantly blocked angiogenesis in in-vitro and ex-vivo models. PIVL exerted these effects by interfering with αvβ3 integrin. In order to produce a biological active recombinant, the cDNA cloning and expression of PIVL was performed in Escherichia coli (BL21)-DE3 cells using pET-22b (+) vector. The recombinant PIVL protein (rPIVL) was purified by nickel affinity chromatography and has recognized monoclonal anti-His antibody. Functionally, rPIVL exhibited potent anti-tumor cell effects as well as anti-angiogenesis properties. Interestingly, we found that both native PIVL (nPIVL) and rPIVL modulated PI3/AKT and MAPK signaling pathways. In all, our results showed that we have successfully expressed the first active anti-oncogenic snake venom Kunitz-type protease inhibitor that can be a potential therapeutic drug against glioblastoma, in its native or recombinant form.
- Published
- 2022
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24. Tunisian Native Mentha pulegium L. Extracts: Phytochemical Composition and Biological Activities.
- Author
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Jebali J, Ghazghazi H, Aouadhi C, ELBini-Dhouib I, Ben Salem R, Srairi-Abid N, Marrakchi N, and Rigane G
- Subjects
- Anti-Infective Agents chemistry, Anti-Infective Agents pharmacology, Antineoplastic Agents, Phytogenic chemistry, Antineoplastic Agents, Phytogenic pharmacology, Antioxidants chemistry, Antioxidants pharmacology, Biological Products chemistry, Biological Products pharmacology, Cell Line, Tumor, Cell Survival drug effects, Chromatography, High Pressure Liquid, Flavonoids, Humans, Phenols, Tunisia, Mentha pulegium chemistry, Phytochemicals chemistry, Phytochemicals pharmacology, Plant Extracts chemistry, Plant Extracts pharmacology
- Abstract
Mint species ( Lamiaceae family) have been used as traditional remedies for the treatment of several diseases. In this work, we aimed to characterize the biological activities of the total phenolic and flavonoid contents of Mentha pulegium L. extracts collected from two different regions of Tunisia. The highest amounts of total phenols (74.45 ± 0.01 mg GAE/g DW), flavonoids (28.87 ± 0.02 mg RE/g DW), and condensed tannins (4.35 ± 0.02 mg CE/g DW) were found in the Bizerte locality. Methanolic leaf extracts were subjected to HPLC-UV analysis in order to identify and quantify the phenolic composition. This technique allowed us to identify seven phenolic compounds: two phenolic acids and five flavonoid compounds, such as eriocitrin, hesperidin, narirutin, luteolin, and isorhoifolin, which were found in both extracts with significant differences between samples collected from the different regions ( p < 0.05). Furthermore, our results showed that the methanolic extract from leaves collected from Bizerte had the highest antioxidant activities (DPPH IC
50 value of 16.31 μg/mL and 570.08 μmol Fe2+ /g, respectively). Both extracts showed high radical-scavenging activity as well as significant antimicrobial activity against eight tested bacteria. The highest antimicrobial activities were observed against Gram-positive bacteria with inhibition zone diameters and MIC values ranging between 19 and 32 mm and 40 and 160 µg/mL, respectively. Interestingly, at 10 μg/mL, the extract had a significant effect on cell proliferation of U87 human glioblastoma cells. These findings open perspectives for the use of Mentha pulegium L. extract in green pharmacy, alternative/complementary medicine, and natural preventive therapies for the development of effective antioxidant, antibacterial, and/or antitumoral drugs.- Published
- 2022
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25. Application of the Paracentrotus lividus sea-urchin embryo-larval bioassay to the marine pollution biomonitoring program in the Tunisian coast.
- Author
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Gharred C, Jenzri M, Bouraoui Z, Guerbej H, Jebali J, and Gharred T
- Subjects
- Animals, Biological Assay, Biological Monitoring, Ecosystem, Humans, Larva, Seawater, Paracentrotus, Water Pollutants, Chemical analysis
- Abstract
The pollution of the marine environment by treated and untreated effluents has increased due to human activities. Monitoring the marine ecosystem is nowadays a global concern. In this work, we evaluated the effect of contaminated and uncontaminated seawater, from different Tunisian coastal areas, on the fertilization, gastrulation, and embryo-larval development events of sea urchins (Paracentrotus lividus). The station of Salakta (SA) is considered as a control station, while the stations of Hamdoun Wadi (HW), Port of Monastir (PM), Karaia Monastir (KM), Teboulba (TE), and Khniss Lagoon (KL) are considered to be contaminated stations. The analysis of seawater physicochemical characteristics showed that levels of the total suspended matter (TSM), chemical oxygen demand (COD), biochemical oxygen demand (BOD), total organic carbon (TOC), and nitrate (NO
3- ) were lower in the seawater of the reference site Salakta (SA) when compared to those of the contaminated seawater sites. In addition, a very strong variation in the levels of trace metals in seawaters sampled in the studied sites was noted. In fact, the highest concentrations of Pb and Cu were observed in Hamdoun Wadi (HW), port of Monastir (PM), and Karaia Monastir (KM), while the highest concentration of Zn was noted in the Teboulba lagoon (TE) and Khniss (LK). Alterations in physicochemical characteristics as well as elevated trace metal levels in the studied seawater samples were correlated with reduced fertility rate, gastrulation rate, and the frequency of normal sea urchin larvae. The total absence of normal sea urchin pluteus larvae in the sea waters of heavily polluted sites proves the great sensitivity of the larval frequency to mixed pollution. This work recommends the utility of urchin fertilization and gastrulation rates and normal pluteus larval frequencies as useful bioassays to monitor the exposure of marine ecosystems to mixed pollution., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)- Published
- 2022
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26. Pharmacological Investigation of CC-LAAO, an L-Amino Acid Oxidase from Cerastes cerastes Snake Venom.
- Author
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Abdelkafi-Koubaa Z, ELBini-Dhouib I, Souid S, Jebali J, Doghri R, Srairi-Abid N, Essafi-Benkhadir K, Micheau O, and Marrakchi N
- Subjects
- Alanine Transaminase metabolism, Animals, Cell Line, Tumor, Cell Survival drug effects, Chick Embryo, Creatinine metabolism, Edema chemically induced, Edema pathology, Hemorrhage chemically induced, Humans, L-Lactate Dehydrogenase metabolism, Male, Mice, L-Amino Acid Oxidase metabolism, Viper Venoms chemistry, Viperidae physiology
- Abstract
Snake venom proteins, which are responsible for deadly snakebite envenomation, induce severe injuries including neurotoxicity, myotoxicity, cardiotoxicity, hemorrhage, and the disruption of blood homeostasis. Yet, many snake-venom proteins have been developed as potential drugs for treating human diseases due to their pharmacological effects. In this study, we evaluated the use of, an L-amino acid oxidase isolated from Cerastes cerastes snake venom CC-LAAO, as a potential anti-glioblastoma drug, by investigating its in vivo and in vitro pharmacological effects. Our results showed that acute exposure to CC-LAAO at 1 and 2.5 µg/mL does not induce significant toxicity on vital organs, as indicated by the murine blood parameters including aspartate transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH) activities, and creatinine levels. The histopathological examination demonstrated that only at high concentrations did CC-LAAO induce inflammation and necrosis in several organs of the test subjects. Interestingly, when tested on human glioblastoma U87 cells, CC-LAAO induced a dose-dependent apoptotic effect through the H
2 O2 generated during the enzymatic reaction. Taken altogether, our data indicated that low concentration of CC-LAAO may be safe and may have potential in the development of anti-glioblastoma agents.- Published
- 2021
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27. Effect of melatonin and folic acid supplementation on the growth performance, antioxidant status, and liver histology of the farmed gilthead sea bream (Sparus aurata L.) under standard rearing conditions.
- Author
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Amri A, Kessabi K, Bouraoui Z, Sakli S, Gharred T, Guerbej H, Messaoudi I, and Jebali J
- Subjects
- Acetylcholinesterase metabolism, Alkaline Phosphatase metabolism, Animals, Catalase metabolism, Fish Proteins metabolism, Insulin-Like Growth Factor I metabolism, Liver drug effects, Liver metabolism, Liver pathology, Superoxide Dismutase metabolism, Thiobarbituric Acid Reactive Substances metabolism, Dietary Supplements, Folic Acid pharmacology, Melatonin pharmacology, Sea Bream growth & development, Sea Bream metabolism
- Abstract
The present study aimed to investigate the effect of dietary of melatonin (MLT) and folic acid (FA) administrations on growth performance, antioxidant status, and liver histological structure of juvenile gilthead sea bream, Sparus aurata L. under standard rearing conditions. Four diets were considered: a basal diet considered a control and three diets supplemented with 40 mg/kg of melatonin (MLT), 2 mg/kg of folic acid (FA), and with the mixture of melatonin and folic acid (MLT + FA). Each diet was randomly allocated to triplicate groups of fish (mean initial weight was 2.99 ± 0.55 g) for 41 days. The obtained results clearly indicated that the melatonin-supplemented diet decreased significantly the growth performance parameters (final body weight, weight gain rate, and specific growth rate) and IGF-1 level of the gilthead sea bream, while the folic acid-supplemented diet has no significant effect on these parameters. The mixture supplementation of melatonin and folic acid has no significant effect on the growth parameters due to the possible interaction between melatonin and folic acid effects. Furthermore, fish fed with all experimental diets showed significantly higher superoxide dismutase activity (SOD) and protein sulfhydryl level (PSH) and lower lipid peroxidation level (TBARS) and catalase activity (CAT) which confirm their powerful antioxidant role. The acetylcholinesterase activity (ACHE) decreased in fish fed with all experimental diets. The underlying mechanisms of driving melatonin and folic acid to reduce acetylcholinesterase activity require further studies. The histological structure of liver of control S. aurata fish shows severe hepatic lipid accumulation in large vacuoles that diminished after dietary individual or mixture folic acid and melatonin supplementations over 41 days. This work proved that 2 mg/kg of dietary folic acid has a positive effect on the growth performance, oxidative stress defense, and hepatic lipid accumulation reduction in the gilthead sea bream fish. Under our experimental conditions, melatonin failed to improve the growth indexes WGR, SGR, and IGF-I. This study recommends the diet supplementation with a dose lower than 2 mg/kg of food due to the observed effects on tissue ACHE activity.
- Published
- 2020
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28. Lebecetin, a snake venom C-type lectin protein, modulates LPS-induced inflammatory cytokine production in human THP-1-derived macrophages.
- Author
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Jebali J, Zakraoui O, Aissaoui D, Abdelkafi-Koubaa Z, Srairi-Abid N, Marrakchi N, and Essafi-Benkhadir K
- Subjects
- Animals, Humans, Interleukin-10 metabolism, Lectins, C-Type, Lipopolysaccharides, NF-kappa B metabolism, Snake Venoms, Viperidae, Cytokines metabolism, Viper Venoms pharmacology
- Abstract
The excessive production of inflammatory mediators results in an overactive immune response leading to the worsening of various human diseases. Thus, there is a still need to identify molecules able to regulate the inflammatory response. Lebecetin, a C-type lectin protein isolated from Macrovipera lebetina snake venom, was previously characterized as a platelet aggregation inhibitor and antitumor active biomolecule. In the present work, we investigated its effect on the production of some cytokines linked to inflammatory response and the underlying mechanisms in lipopolysaccharide (LPS)-induced THP1 macrophages. Interestingly, we found that lebecetin reduced the levels of the pro-inflammatory cytokines TNF-α, IL-6, and IL-8 while it partially increased LPS-induced secretion of the immunomodulatory cytokine IL-10. Furthermore, this modulatory effect was accompanied by decreased activation of ERK1/2, p38, AKT kinases and NF-κB along with reduced expression of αvβ3 integrin. Thus, this study highlights the promising role of lebecetin as a natural biomolecule that could manage the inflammatory response involved in the development and progression of inflammatory diseases., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2020
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29. Incidence of morphometry variation, growth alteration, and reproduction performance of the annular sea bream (Diplodus annularis) as effective tools to assess marine contamination: how useful is a multi-biotimarkers approach?
- Author
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Gharred T, Mannai R, Belgacem M, and Jebali J
- Subjects
- Animals, Female, Incidence, Male, Mediterranean Sea, Reproduction, Perciformes metabolism, Sea Bream metabolism
- Abstract
A wide variety of contaminants in the aquatic environment causes several deleterious effects on aquatic organism traits ranging from molecular to individual and population levels. This in situ study investigated morphometry, growth performance, and reproduction success of a teleost fish, Diplodus annularis, from a polluted site "Sayada" and a reference site "Salakta" in the eastern Tunisian coastline. Morphometric indexes, generalized Procruste analysis, thin plate, and otolith contour methods were used to assess the pollution effects on morphological traits. The growth performance of fish from contaminated and control sites were studied using the Von Bertalanffy equation. Gonadosomatic (GSI) and Hepatosomatic (HSI) Indexes and absolute and relative fecundity were used to assess the impact of pollution on the reproduction success of this species. The obtained results showed that the fish of the contaminated zone had significant alteration of the morphology, slower growth, significant increase in the HSI, significant decrease in female GSI, and a decrease in absolute and relative fecundity compared to specimens from the unpolluted zone. Based on these results, the biological traits of Diplodus annularis can be used as biological biomarkers in the monitoring and protection programs of the marine contamination in the Mediterranean Sea.
- Published
- 2020
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30. Targeting α1 inserted domain (I) of α1β1 integrin by Lebetin 2 from M. lebetina transmediterranea venom decreased tumorigenesis and angiogenesis.
- Author
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Morjen M, Othman H, Abdelkafi-Koubaa Z, Messadi E, Jebali J, El Ayeb M, Abid NS, Luis J, and Marrakchi N
- Subjects
- Amino Acid Sequence, Angiogenesis Inhibitors metabolism, Angiogenesis Inhibitors pharmacology, Angiogenesis Inhibitors therapeutic use, Animals, CHO Cells, Cell Adhesion drug effects, Cell Movement drug effects, Cell Proliferation drug effects, Cricetulus, PC12 Cells, Protein Domains, Rats, Viper Venoms metabolism, Viper Venoms pharmacology, Viper Venoms therapeutic use, Carcinogenesis drug effects, Integrin alpha1beta1 chemistry, Integrin alpha1beta1 metabolism, Neovascularization, Pathologic drug therapy, Viper Venoms chemistry
- Abstract
Through the recent development of knowledge in biotechnology and bioinformatics, snake venoms are widely used to develop new drugs to treat diseases such as hypertension and cancer. We have previously reported that Lebetin 2 isolated from Macrovipera lebetina transmediterranea venom displays a potent anti-platelet activity and exerts a cardioprotective effect in ischemia-reperfusion (IR) injury model. Here, we report that Lebetin 2 possess an anti-tumor effect by targeting the integrin receptor function. It was thus able to inhibit both adhesion and migration of pheochromocytoma cells (PC12) and α1β1 integrin-expressing CHO cells (CHO-α1) to type I and IV collagens. Moreover, this peptide affects proliferation of PC12 cells by modulating AKT phosphorylation. Furthermore, Lebetin 2 exhibits a potent anti-angiogenic effect as assessed in vitro and ex vivo, using both the embryo chick chorioallantoic membrane model (CAM) and rat aortic ring assay. Interestingly, the interaction mode of Lebetin 2 with the integrin α1β1, assessed in silico, showed that the peptide represents a steric obstruction preventing the collagen from enforcing the interactions with the integrin., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
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31. BotAF, a new Buthus occitanus tunetanus scorpion toxin, produces potent analgesia in rodents.
- Author
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Maatoug R, Jebali J, Guieu R, De Waard M, and Kharrat R
- Subjects
- Analgesics chemistry, Analgesics toxicity, Animals, Female, Male, Mice, Inbred C57BL, Pain drug therapy, Rats, Wistar, Scorpion Venoms chemistry, Scorpion Venoms toxicity, Sequence Analysis, Protein, Toxicity Tests, Analgesics pharmacology, Scorpion Venoms pharmacology, Scorpions chemistry
- Abstract
This work reports the purification of new potent scorpion neuropeptide, named BotAF, by an activity-guided screening approach. BotAF is a 64-residue long-chain peptide that shares very high similarity with the original β-like scorpion toxin group, in which several peptides have been characterized to be anti-nociceptive in rodents. BotAF administration to rodents does not produce any toxicity or motor impairment, including at high doses. In all models investigated, BotAF turned out to be an efficient peptide in abolishing acute and inflammatory (both somatic and visceral) pain in rodents. It performs with high potency compared to standard analgesics tested in the same conditions. The anti-nociceptive activity of BotAF depends on the route of injection: it is inactive when tested by i.c.v. or i.v. routes but gains in potency when pre-injected locally (in the same compartment than the irritant itself) or by i.t. root 40 to 60 min before pain induction, respectively. BotAF is not an AINS-like compound as it fails to reduce inflammatory edema. Also, it does not activate the opioidergic system as its activity is not affected by naloxone. BotAF does also not bind onto RyR and has low activity towards DRG ion channels (particularly TTX sensitive Na
+ channels) and does not bind onto rat brain synaptosome receptors. In somatic and visceral pain models, BotAF dose-dependently inhibited lumbar spinal cord c-fos/c-jun mRNA up regulation. Altogether, our data favor a spinal or peripheral anti-nociceptive mode of action of BotAF., (Copyright © 2018. Published by Elsevier Ltd.)- Published
- 2018
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32. Helix aspersa maxima mucus exhibits antimelanogenic and antitumoral effects against melanoma cells.
- Author
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Ellijimi C, Ben Hammouda M, Othman H, Moslah W, Jebali J, Mabrouk HB, Morjen M, Haoues M, Luis J, Marrakchi N, Essafi-Benkhadir K, and Srairi-Abid N
- Subjects
- Animals, Apoptosis drug effects, Carcinogenesis drug effects, Carcinogenesis pathology, Cell Adhesion drug effects, Cell Line, Tumor, Cell Movement drug effects, Cell Survival drug effects, Humans, Matrix Metalloproteinase 2 metabolism, Matrix Metalloproteinase Inhibitors pharmacology, Matrix Metalloproteinase Inhibitors therapeutic use, Melanins metabolism, Melanoma pathology, Neoplasm Invasiveness, Helix, Snails chemistry, Melanoma drug therapy, Mucus metabolism
- Abstract
Snail secretion is currently revolutionizing the world of cosmetics and human skin care. The efficacy of snail secretion in wounds healing has been proven both in vitro and by clinical studies. However, the potential anti-tumor effect of snail secretion was poorly investigated. In this report, our in vitro study showed that Helix aspersa maxima species snail slime (SS) could not only treat melanogenesis but also endowed with anti-tumoral activity against human melanoma cells. Indeed, SS reduced melanin content and tyrosinase activity on B16F10 cells with IC
50 values of 288 μg/mL and 286 μg/mL, respectively, without altering cell viability. This effect was also observed, at a lesser extent, on human melanoma IGR-39 and SK-MEL-28 cell lines. On another hand, SS specifically inhibited the viability of IGR-39 and SK-MEL-28 cells associated to an apoptotic effect highlighted by PARP cleavage. It is worth to note that SS did not affect the viability of B16F10 cells and non tumorigenic HaCaT cells. Interestingly, this extract was found to inhibit migration and invasion of both human melanoma cells through reducing the expression of Matrix metalloproteinase MMP2. Snail slime also exerted a high inhibitory effect on IGR-39 cell adhesion through blocking the function of α2 β1 (45%), αv β3 (38%) integrins and by reducing the expression levels of αv and β1 integrins. The presented results shed light on the potential anti-melanoma effect of SS and support its use against skin diseases., (Copyright © 2018 Elsevier Masson SAS. All rights reserved.)- Published
- 2018
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33. Functional role of Kv1.1 and Kv1.3 channels in the neoplastic progression steps of three cancer cell lines, elucidated by scorpion peptides.
- Author
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Aissaoui D, Mlayah-Bellalouna S, Jebali J, Abdelkafi-Koubaa Z, Souid S, Moslah W, Othman H, Luis J, ElAyeb M, Marrakchi N, Essafi-Benkhadir K, and Srairi-Abid N
- Subjects
- Amino Acid Sequence genetics, Animals, Carcinogenesis drug effects, Cell Line, Tumor, Cell Proliferation drug effects, Gene Expression Regulation, Neoplastic drug effects, Humans, Neoplasms drug therapy, Neoplasms genetics, Peptides chemistry, Peptides pharmacology, Potassium metabolism, Potassium Channel Blockers chemistry, Scorpion Venoms chemistry, Scorpions chemistry, Kv1.3 Potassium Channel genetics, Potassium Channel Blockers pharmacology, Scorpion Venoms pharmacology, Shaker Superfamily of Potassium Channels genetics
- Abstract
Voltage-gated potassium (Kv) channels are known to play a pivotal role in the progression of various cancer types and considered as new targets for designing anti-cancer therapy. However, the fact that many Kv channels are expressed in different cell lines makes it difficult to ascribe a functional role for a given Kv channel on a specific aspect of the tumorogenesis. In this work, we showed that although both Kv1.1 and Kv1.3 channels are expressed in U87 (glioblastoma), MDA-MB-231 (breast cancer) and LS174 (colon adenocarcinoma) cells, these respond differently to KAaH1 or KAaH2, two homologous Kv1 blockers from scorpion venom. KAaH1 is active on Kv1.1 and Kv1.3 and was found to inhibit migration and adhesion of U87 cells whereas KAaH2 which is slightly active only on Kv1.1 channel, inhibits their proliferation via the EGFR signaling pathway. The correlation between the electro-physiological activity of the scorpion peptides and their anti-migratory effects suggests the involvement of the Kv1.1 and Kv1.3 channels in the mobility of the three cancer cell lines. Our results showed that besides they can elucidate the implication of Kv1.1 and Kv1.3 channels in molecular mechanisms of neoplastic progression, KAaH1 and KAaH2 may be used as therapeutic tools against glioblastoma., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
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34. Assessment of the individual and mixture toxicity of cadmium, copper and oxytetracycline, on the embryo-larval development of the sea urchin Paracentrotus lividus.
- Author
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Gharred T, Jebali J, Belgacem M, Mannai R, and Achour S
- Subjects
- Animals, Drug Interactions, Embryo, Nonmammalian drug effects, Female, Fertilization drug effects, Larva drug effects, Larva growth & development, Male, Paracentrotus growth & development, Sea Urchins, Toxicity Tests, Cadmium toxicity, Copper toxicity, Oxytetracycline toxicity, Paracentrotus drug effects
- Abstract
Multiple pollutions by trace metals and pharmaceuticals have become one of the most important problems in marine coastal areas because of its excessive toxicity on organisms living in this area. This study aimed to assess the individual and mixture toxicity of Cu, Cd, and oxytetracycline frequently existing in the contaminated marine areas and the embryo-larval development of the sea urchin Paracentrotus lividus. The individual contamination of the spermatozoid for 1 h with the increasing concentrations of Cd, Cu, and OTC decreases the fertility rate and increases larvae anomalies in the order Cu > Cd > OTC. Moreover, the normal larva frequency and the length of spicules were more sensitive than the fertilization rate and normal gastrula frequency endpoints. The mixture toxicity assessed by multiple experimental designs showed clearly that concentrations of Cd, Cu, and OTC superior to 338 μg/L, 0.56 μg/L, and 0.83 mg/L, respectively, cause significant larva malformations.
- Published
- 2016
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35. Using environmental proteomics to assess pollutant response of Carcinus maenas along the Tunisian coast.
- Author
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Ghedira J, Chicano-Gálvez E, Fernández-Cisnal R, Jebali J, Banni M, Chouba L, Boussetta H, López-Barea J, and Alhama J
- Subjects
- Animals, Biomarkers metabolism, Gills metabolism, Metallothionein metabolism, Proteomics, Tunisia, Vitellogenins metabolism, Water Pollutants, Chemical analysis, Brachyura metabolism, Environmental Monitoring methods, Proteome metabolism, Water Pollutants, Chemical metabolism
- Abstract
Biochemical responses to pollutants were studied at four Tunisia littoral sites using Carcinus maenas as a bioindicator. Proteomic analysis was used to assess the global impact of complex pollution mixtures, and to provide new biomarkers and basic insights into pollutant toxicity. Metal contents and metallothionein levels followed a gradient based on sampling sites: Bizerte ≫ Teboulba > Gargour~Mahres. Approximately 900 and 700 spots were resolved in digestive glands and gills, respectively. Gills from Bizerte animals had the maximum number of altered spots, mostly upregulated. In other locations, the number of altered spots in gills decreased in parallel to total metals in in the following order: Teboulba > Gargour > Mahres (mostly downregulated). Out of the 39 spots excised, ten proteins were identified in digestive glands and eight in gills. Digestive glands of Bizerte crabs had higher levels of ferritin, three vitellogenin forms and mannose-binding protein, while Gargour crabs had higher levels of four cryptocyanin forms. Gills of Bizerte crabs had higher levels of ferritin, three vitellogenins forms, lectin 4C, actin, and collagenolytic serine protease. Proteins with altered expression in crabs from Tunisia littoral are related to molting, oxidative stress and inflammation, innate immune response, and proteolysis., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2016
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36. Assessment of toxic interactions between deltamethrin and copper on the fertility and developmental events in the Mediterranean sea urchin, Paracentrotus lividus.
- Author
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Gharred T, Ezzine IK, Naija A, Bouali RR, and Jebali J
- Subjects
- Animals, Copper chemistry, Embryo, Nonmammalian drug effects, Embryo, Nonmammalian physiology, Embryonic Development drug effects, Environmental Monitoring, Fertility, Fertilization drug effects, Insecticides chemistry, Larva drug effects, Male, Mediterranean Sea, Nitriles chemistry, Pyrethrins chemistry, Seawater, Spermatozoa drug effects, Water Pollutants, Chemical chemistry, Copper toxicity, Insecticides toxicity, Nitriles toxicity, Paracentrotus physiology, Pyrethrins toxicity, Water Pollutants, Chemical toxicity
- Abstract
Deltamethrin pesticide and copper have intensively been used in agriculture and industrial activities and can finally reach the marine ecosystem at high concentrations affecting the health of organisms. In this study, we assessed under laboratory conditions the toxic interactions between deltamethrin and copper and their effects on the fertility rate, cell mitotic division rate, and embryo developmental events of the sea urchin (Paracentrotus lividus). The exposure of sperm to increasing concentrations of deltamethrin (6.10(-5) and 6.10(-2) μg/L) and copper (50 and 100 μg/L) or to their mixture (6.10(-5) μg/L of deltamethrin and 50 μg/L of CuSO4) caused a significant alteration on the fertilizing capability of spermatozoids. Concentration-dependent toxic effects on the early cleavage in P. lividus were observed in groups treated with copper, deltamethrin, and their mixture. The kinetics of early divisions was accelerated and the average size of pluteus larvae was decreased under pollutant treatments. Several developmental anomalies were identified in pluteus, including crossed skeletal tips at the hood apex, joined or atrophied arms, and alteration of general larva shape. In conclusion, the sea urchin represents a suitable and sensitive model for testing the toxicity and the effects of deltamethrin pesticide and copper in sea water. In addition, the sensitivity of various end points to studied contaminants, proved their utility in the infield biomonitoring studies.
- Published
- 2015
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37. A thermoactive L-amino acid oxidase from Cerastes cerastes snake venom: purification, biochemical and molecular characterization.
- Author
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Abdelkafi-Koubaa Z, Jebali J, Othman H, Morjen M, Aissa I, Zouari-Kesentini R, Bazaa A, Ellefi AA, Majdoub H, Srairi-Abid N, Gargouri Y, El Ayeb M, and Marrakchi N
- Subjects
- Amino Acid Sequence, Base Sequence, Chromatography, Affinity, Chromatography, Gel, Chromatography, Ion Exchange, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Glycosylation, Kinetics, L-Amino Acid Oxidase isolation & purification, Molecular Sequence Data, Sequence Alignment, Sequence Analysis, Protein, Substrate Specificity, L-Amino Acid Oxidase chemistry, Viper Venoms chemistry
- Abstract
A new L-amino acid oxidase (LAAO) from Cerastes cerastes snake venom, named CC-LAAO, was purified to homogeneity using a combination of size-exclusion, ion-exchange and affinity chromatography. CC-LAAO is a homodimeric glycosylated flavoprotein with a molecular mass around 58 kDa under reducing conditions and about 115 kDa in its native form when analyzed by SDS-PAGE and gel filtration chromatography, respectively. This enzyme displayed a Michaelis-Menten behavior with an optimal pH at 7.8. However, unlike known SV-LAAOs which display their maximum activity at 37 °C, CC-LAAO has an optimal temperature at 50 °C. Kinetic studies showed that the enzyme displayed high specificity towards hydrophobic L-amino acids. The best substrates were L-Phe, L-Met and L-Leu. CC-LAAO activity was inhibited by the substrate analog N-acetyl tryptophan. The N-terminal amino acid sequence of this protein was determined by automated Edman degradation. The CC-LAAO cDNA was cloned from the venom gland total RNA preparation. The cDNA sequence contained an open-reading frame (ORF) of 1551-bp, which encoded a protein of 516 amino acids comprising a signal peptide of 18 amino acids and 498-residues mature protein. CC-LAAO sequence and its tertiary model shared high similarity with other snake venom LAAOs., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
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38. Lebecin, a new C-type lectin like protein from Macrovipera lebetina venom with anti-tumor activity against the breast cancer cell line MDA-MB231.
- Author
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Jebali J, Fakhfekh E, Morgen M, Srairi-Abid N, Majdoub H, Gargouri A, El Ayeb M, Luis J, Marrakchi N, and Sarray S
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Breast Neoplasms pathology, Cell Adhesion drug effects, Cell Line, Tumor, Cell Movement drug effects, Cloning, Molecular, Female, Humans, Lectins, C-Type chemistry, Lectins, C-Type isolation & purification, Models, Molecular, Molecular Sequence Data, Protein Structure, Tertiary, Sequence Alignment, Sequence Analysis, Protein, Viper Venoms chemistry, Viper Venoms isolation & purification, Cell Proliferation drug effects, Viper Venoms pharmacology, Viperidae
- Abstract
C-type lectins like proteins display various biological activities and are known to affect especially platelet aggregation. Few of them have been reported to have anti-tumor effects. In this study, we have identified and characterized a new C-type lectin like protein, named lebecin. Lebecin is a heterodimeric protein of 30 kDa. The N-terminal amino acid sequences of both subunits were determined by Edman degradation and the entire amino acid sequences were deduced from cDNAs. The precursors of both lebecin subunits contain a 23-amino acid residue signal peptide and the mature α and β subunits are composed of 129 and 131 amino acids, respectively. Lebecin is shown to be a potent inhibitor of MDA-MB231 human breast cancer cells proliferation. Furthermore, lebecin dose-dependently inhibited the integrin-mediated attachment of these cells to different adhesion substrata. This novel C-type lectin also completely blocked MDA-MB231 cells migration towards fibronectin and fibrinogen in haptotaxis assays., (Copyright © 2014. Published by Elsevier Ltd.)
- Published
- 2014
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39. Comparative study of the bioaccumulation and elimination of trace metals (Cd, Pb, Zn, Mn and Fe) in the digestive gland, gills and muscle of bivalve Pinna nobilis during a field transplant experiment.
- Author
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Jebali J, Chouba L, Banni M, and Boussetta H
- Subjects
- Animals, Seawater, Time Factors, Tissue Distribution, Bivalvia metabolism, Digestive System metabolism, Environmental Monitoring, Gills metabolism, Muscles metabolism, Trace Elements metabolism
- Abstract
The purpose of this study was to investigate the long-term bioaccumulation and elimination of Cd, Pb, Mn, Zn and Fe by Pinna nobilis tissues after their 90 day-transplantation period at Téboulba fishing harbor. During the transplantation period, the Cd, Pb, Mn, Zn and Fe concentrations in the different tissues of the mussels were measured before and after exposure period. Metal (Cd, Pb, Mn, Zn and Fe) accumulation in P. nobilis mussels varied depending on the analyzed tissue and the caging times. Notable differences in Cd, Pb, Mn, Zn and Fe accumulation patterns within the digestive gland, gills and muscle were found and may be due to the ability of each tissue to accumulate metals. During the depuration phase, the elimination of Cd, Pb, Mn, Zn and Fe depended on the target tissue and the metal speciation. Cd, Pb, Mn and Fe were eliminated rapidly from one organ and increased in other when compared to those of 90 day transplanted mussels. The increase of metal loads during the elimination phase is not clear and particularly what kind of processes is responsible for such response. However, it is reasonable to assume that metals increase is related to the existence of an accumulation/detoxification mechanism, which involves the transport of metals from an organ to another. The data obtained indicate that because of the significantly high quantities of Cd, Pb, Mn, Zn and Fe accumulated during the exposure phase, the transplanted mussels are suitable bioindicators for monitoring trace metals in marine ecosystem., (Copyright © 2013 Elsevier GmbH. All rights reserved.)
- Published
- 2014
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40. Metals bioaccumulation and histopathological biomarkers in Carcinus maenas crab from Bizerta lagoon, Tunisia.
- Author
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Ben-Khedher S, Jebali J, Houas Z, Nawéli H, Jrad A, Banni M, and Boussetta H
- Subjects
- Animals, Biomarkers metabolism, Gills metabolism, Metals toxicity, Tunisia, Water Pollutants, Chemical toxicity, Brachyura physiology, Environmental Monitoring methods, Metals metabolism, Water Pollutants, Chemical metabolism
- Abstract
Metals concentrations and histolopathological lesions of gills and digestive gland were investigated in Carcinus maenas crabs sampled from Bizerta Lagoon and Kuriat Island (Tunisia) as control site. The concentrations of trace metals varied between tissues, sites and sampling time. The highest levels of the analysed metals in gills and digestive gland were noted in Menzel Bourguiba and Cimentery sites at both sampling times (February and July). The higher metals loads were associated with severe and various tissues alterations in contaminated crabs. We particularly noted in the gills a haemocytic infiltration, distension and enlargement of the lamellae, lifting of lamellar epithelium, necrotic lesions and fusion of lamellae in the most polluted sites (Menzel Bourguiba and Cimentery). Moreover, others pathological alterations were observed in digestive gland of crabs collected from polluted sites and with a severity site dependent. We observed necrotic tubules containing tissue debris in the lumen with more intensity in crabs collected from Cimentery site in both sampling times. The thickened basal laminae and the walling off of the tubules by haemocytes around the thickened basal laminae were more abundant at Menzel Bourguiba than at others sites. The coagulation in the thickened basal laminae was observed only at Cimentery in February. Tissues histopathological lesions were sensitive to discriminate crabs of different sites and demonstrated its usefulness in this biomonitoring study. We recommend the association of histopatholocial lesions to biochemical biomarkers in future biomonitoring studies.
- Published
- 2014
- Full Text
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41. Effects of thermal stress and nickel exposure on biomarkers responses in Mytilus galloprovincialis (Lam).
- Author
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Attig H, Kamel N, Sforzini S, Dagnino A, Jamel J, Boussetta H, Viarengo A, and Banni M
- Subjects
- Animals, Enzyme Activation drug effects, Mytilus enzymology, Biomarkers analysis, Hot Temperature, Mytilus drug effects, Mytilus physiology, Nickel toxicity, Stress, Physiological, Water Pollutants, Chemical toxicity
- Abstract
The present work aimed to assess the Mytilus galloprovincialis digestive gland biomarkers responses to nickel (Ni) exposure along with a heat stress gradient. Mussels were exposed to a sublethal dose of nickel (13 μM) along with a temperature gradient (18 °C, 20 °C, 22 °C, 24 °C and 26 °C) for 4 days. Metallothionein (MTs) content was assessed as specific response to metals. Catalase (CAT), glutathione S-transferase (GST) activities and malondialdehyde (MDA) were measured as biomarkers of oxidative stress and lipid peroxidation. The cholinergic system was monitored using the acetylcholinesterase activity (AChE). Moreover, Ni uptakes along with the exposure temperatures were assessed. A correlation matrix (CM) between the investigated biomarkers and the exposure temperatures and a Principal Component Analysis (PCA) were achieved. Our data showed a negative effect of temperature increase on mussel's antioxidant and detoxification response to Ni exposure being more pronounced in animals exposed to the 24 °C and 26 °C., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2014
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42. Proteomic analysis in caged Mediterranean crab (Carcinus maenas) and chemical contaminant exposure in Téboulba Harbour, Tunisia.
- Author
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Jebali J, Chicano-Gálvez E, Fernández-Cisnal R, Banni M, Chouba L, Boussetta H, López-Barea J, and Alhama J
- Subjects
- Animals, Biomarkers metabolism, Environmental Monitoring, Tandem Mass Spectrometry, Tunisia, Water Pollutants, Chemical analysis, Water Pollutants, Chemical toxicity, Brachyura drug effects, Environmental Exposure, Proteomics
- Abstract
This study uses proteomics approach to assess the toxic effects of contaminants in the Mediterranean crab (Carcinus maenas) after transplantation into Téboulba fishing harbour. High levels of aliphatic and aromatic hydrocarbons were detected in sediments. Although their effects on vertebrates are well described, little is known about their early biological effects in marine invertebrates under realistic conditions. Protein expression profiles of crabs caged for 15, 30 and 60 days were compared to unexposed animals. Nineteen proteins with significant expression differences were identified by capLC-µESI-IT MS/MS and homology search on databases. Differentially expressed proteins were assigned to five different categories of biological function including: (1) chitin catabolism, (2) proteolysis, (3) exoskeleton biosynthesis, (4) protein folding and stress response, and (5) transport. The proteins showing major expression changes in C. maenas after different caging times may be considered as novel molecular biomarkers for effectively biomonitoring aquatic environment contamination., (© 2013 Elsevier Inc. All rights reserved.)
- Published
- 2014
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43. Multiple biomarkers of pollution effects in Solea solea fish on the Tunisia coastline.
- Author
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Jebali J, Sabbagh M, Banni M, Kamel N, Ben-Khedher S, M'hamdi N, and Boussetta H
- Subjects
- Acetylcholinesterase analysis, Acetylcholinesterase metabolism, Animals, Catalase analysis, Catalase metabolism, Gills drug effects, Gills metabolism, Glutathione Transferase analysis, Glutathione Transferase metabolism, Lipid Peroxidation drug effects, Metallothionein analysis, Metallothionein metabolism, Oxidative Stress drug effects, Tunisia, Biomarkers analysis, Environmental Monitoring methods, Environmental Pollution analysis, Flatfishes metabolism
- Abstract
This field study investigates the morphological indices (condition index, hepatosomatic index) and biochemical (catalase (CAT), glutathione S-transferase (GST), acetylcholinesterase (AChE), metallothionein (MT), lipid peroxidation) parameters in liver, gills and kidney of common sole (Solea solea) originating from different sites of the Tunisian coast area impacted by different anthropogenic activities. Differences among sites and tissues for AChE, GST, CAT, MT and TBARS were found and possibly related to known sources of domestic and industrial discharges in the studied sites. Liver, gills and kidney CAT, liver and kidney MT and brain AChE were key biomarkers to discriminate fish of different sites. So, we suggest using these biomarkers in future biomonitoring.
- Published
- 2013
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44. Biochemical effects in crabs (Carcinus maenas) and contamination levels in the Bizerta Lagoon: an integrated approach in biomonitoring of marine complex pollution.
- Author
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Ben-Khedher S, Jebali J, Kamel N, Banni M, Rameh M, Jrad A, and Boussetta H
- Subjects
- Animals, Biomarkers metabolism, Brachyura metabolism, Digestive System metabolism, Ecotoxicology standards, Environmental Monitoring standards, Gas Chromatography-Mass Spectrometry, Gills metabolism, Hydrocarbons analysis, Hydrocarbons toxicity, Metals analysis, Metals toxicity, Quality Control, Seasons, Spectrum Analysis, Tunisia, Water Pollutants, Chemical analysis, Water Pollution, Chemical analysis, Brachyura drug effects, Ecotoxicology methods, Environmental Monitoring methods, Geologic Sediments chemistry, Oceans and Seas, Water Pollutants, Chemical toxicity, Water Pollution, Chemical adverse effects
- Abstract
The biochemical effects in Carcinus maenas and contamination levels in seawater and sediments of Bizerta Lagoon (northeast of Tunisia) were investigated. The levels of metals and hydrocarbons were higher in seawater and sediments in Menzel Bourguiba and Cimentery in February and July than in the other sampling sites. Differences among sites for glutathione S-transferase, catalase, acetylcholinesterase activities, and the content of lipid peroxidation and metallothioneins in two important organs which accumulated contaminants (the gills and the digestive gland) of the C. maenas were found and possibly related to differences in metal and hydrocarbon levels. The seasonal variation of biomarkers was possibly associated with chemical contamination and also with the high fluctuation of physico-chemical characteristics of the sites. The integrated biomarker response values found in the five sites is in good agreement with hydrocarbon and trace metal concentrations detected in the water and sediments of the stressful places where crabs are living.
- Published
- 2013
- Full Text
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45. Epidemiological study of sympatric Haemonchus species and genetic characterization of Haemonchus contortus in domestic ruminants in Tunisia.
- Author
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Akkari H, Jebali J, Gharbi M, Mhadhbi M, Awadi S, and Darghouth MA
- Subjects
- Abattoirs, Animals, Animals, Domestic, Cloning, Molecular, DNA, Helminth genetics, DNA, Ribosomal Spacer genetics, Female, Haemonchiasis epidemiology, Haemonchiasis parasitology, Haemonchus classification, Male, Phylogeny, Species Specificity, Tunisia epidemiology, Haemonchiasis veterinary, Haemonchus genetics, Haemonchus isolation & purification, Ruminants
- Abstract
Parasitic gastroenteritis caused by Haemonchus spp. is a major cause of economic losses in the livestock industry, especially in tropical and subtropical areas. The correct identification of various species, as well as knowledge regarding the epidemiology and genetic characterization of the principal circulating species, is essential for the establishment of sustainable control strategies. A study was carried out to determine the prevalence of Haemonchus species in sheep, goats and cattle slaughtered in Béja abattoir from January to June 2010 and also to analyse the genetic differences of Haemonchus contortus in these ruminants. During the study period 364, 271 and 152 abomasa of sheep, goats and cattle respectively, were examined showing overall prevalence rates of 17%, 33.6% and 7.23%, respectively. In addition, spicules morphometric study of 300 male worms randomly collected from sheep showed the presence of 239 (79.66%) H. contortus and 61 (20.33%) H. placei. Likewise, out of 508 adult male Haemonchus from goats, 325 (63.97%) H. contortus and 183 (36.02%) H. placei worms were identified. Whereas for cattle, out of 84 adult male Haemonchus, 52 (61.9%) H. contortus and 32 (38.09%) H. placei worms were identified. The study showed the association of H. contortus and H. placei as a predominant type of infection in all hosts, co-infection concerned 62.5% of sheep, 54.71% of goats and 37.5% of cattle. Using the polymerase chain reaction, the second Internal Transcribed Spacer region (ITS-2) of the nuclear ribosomal DNA (rDNA) of H. contortus was amplified and sequenced. A total of 16 ITS-2 sequences were identified (five from sheep, three from cattle and eight from goats). The 231 base pairs of different ITS-2 sequences were aligned and analysed. Distance based analysis using Neighbour-Joining method and parsimony analysis were used to construct phylogenetic trees to elucidate genetic relationships. The analyses categorized the ITS-2 sequence of H. contortus into four groups. Groups 1 and 4 were found exclusively in goats, whereas groups 2 and 3 were found in sheep and cattle. This study demonstrates variability in nucleotide sequence within the ITS-2 region that reveals genetic diversity among populations of H. contortus, including those from different domestic ruminant species in Tunisia. To our knowledge, this is the first work in North Africa describing the genetic diversity of H. contortus in domestic ruminants., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
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46. Biochemical responses in seabream (Sparus aurata) caged in-field or exposed to benzo(a)pyrene and paraquat. Characterization of glutathione S-transferases.
- Author
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Jebali J, Chicano-Gálvez E, Banni M, Guerbej H, Boussetta H, López-Barea J, and Alhama J
- Subjects
- Animals, Biomarkers metabolism, Biotransformation, Cytochrome P-450 CYP1A1 metabolism, Enzymes metabolism, Glutathione metabolism, Oxidative Stress drug effects, Proteomics, Time Factors, Benzo(a)pyrene toxicity, Gene Expression Regulation, Enzymologic drug effects, Glutathione Transferase metabolism, Paraquat toxicity, Sea Bream physiology, Water Pollutants, Chemical toxicity
- Abstract
Gilthead seabream (Sparus aurata) specimens were caged in-field at the Téboulba harbour or exposed to benzo(a)pyrene [B(a)P] or to paraquat [PQ] plus B(a)P, and several biochemical biomarker responses were investigated. Antioxidant enzymes, such as glutathione peroxidase, catalase and glutathione reductase, significantly increased in the in-field and B(a)P+PQ exposures, but were only moderately affected by B(a)P alone. Glucose-6-phosphate and 6-phosphogluconate dehydrogenases significantly diminished after in-field exposure. Different responses with biotransformation enzymes were observed: the P4501A-associated EROD activity was highly induced in response to B(a)P and B(a)P+PQ exposures, while total activity of the glutathione S-transferase (GST) was similar to control. However, after purification of the GST proteins by affinity chromatograpy and analysis by two-dimensional electrophoresis, nineteen highly reproducible isoforms were resolved. In addition, some of reproducible isoforms showed different and specific expression patterns in response to contaminants. Thus, proteomic analysis of the purified GST subunits is a reliable tool for ecotoxicological research, useful in polluted marine ecosystem as an effective biomarker of contamination., (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
47. Expression of a functional recombinant C-type lectin-like protein lebecetin in the human embryonic kidney cells.
- Author
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Jebali J, Jeanneau C, Morjen M, Mathieu S, Bazaa A, El Ayeb M, Luis J, Gargouri A, Marrakchi N, and el Battari A
- Subjects
- Animals, Blotting, Western, CHO Cells, Cell Line, Tumor, Cell Movement drug effects, Cell Proliferation drug effects, Cricetinae, Cricetulus, Embryo, Mammalian cytology, HEK293 Cells, Humans, Integrins metabolism, Kidney cytology, Lectins, C-Type chemistry, Lectins, C-Type genetics, Protein Subunits, Recombinant Proteins biosynthesis, Recombinant Proteins chemistry, Recombinant Proteins genetics, Recombinant Proteins pharmacology, Viper Venoms chemistry, Viper Venoms genetics, Viper Venoms pharmacology, Kidney metabolism, Lectins, C-Type biosynthesis, Viper Venoms biosynthesis
- Abstract
Lebecetin is an anticoagulant C-type lectin-like protein that was previously isolated from Macrovipera lebetina venom and described to consist of two subunits (alpha and beta). It was reported to potently prevent platelet aggregation by binding to glycoprotein Ib and to exhibit a broad spectrum of inhibitory activities on various integrin-mediated functions of tumor cells, including adhesion, proliferation, and cell migration. This study aimed to investigate the structure-function of lebecetin. Accordingly, the cDNA of each subunit was cloned and separately or jointly expressed in the human embryonic kidney cells using two vectors with different selectable tags. The immunofluorescence analysis of transfected cells revealed significant expression levels and co-localization of the two lebecetin subunits. The recombinant proteins were efficiently secreted and purified using metal-chelating affinity chromatography. We found that the Lebecetin alpha and beta subunits were produced as a mixture of homodimers and heterodimers and that the heterodimerization represents a prerequisite for functioning., (Copyright © 2012 American Institute of Chemical Engineers (AIChE).)
- Published
- 2012
- Full Text
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48. Biochemical responses and metals levels in Ruditapes decussatus after exposure to treated municipal effluents.
- Author
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Kamel N, Jebali J, Banni M, Ben Khedher S, Chouba L, and Boussetta H
- Subjects
- Animals, Biomarkers metabolism, Catalase metabolism, Environmental Monitoring, Gills chemistry, Gills drug effects, Glutathione Transferase metabolism, Lipid Peroxidation drug effects, Metallothionein metabolism, Metals, Heavy toxicity, Oxidative Stress drug effects, Tunisia, Wastewater analysis, Bivalvia chemistry, Bivalvia drug effects, Wastewater toxicity, Water Pollutants, Chemical toxicity
- Abstract
This study assessed the responses of biochemical biomarkers and metals levels in Ruditapes decussatus exposed to the increasing concentrations of treated municipal effluents (TME) discharged into the Tunisian coastal area. Clams were exposed to 0%, 1%, 3% and 10% for 7 and 14 day and the following biochemical responses were measured: (1) catalase activity and lipid peroxidation levels (TBARS) as oxidative stress biomarkers, (2) gluthathione S-transferase (GST) activity as a phase II conjugation enzyme; (3) cholinesterase activity (ChE) as biomarker of neurotoxicity, and (4) metallothioneins as a proteins highly induced by heavy metals. A significant uptake of Cu, Cd and Zn in digestive gland and serious biochemical alterations were observed. Thus, exposure of clams to croissant concentration of TME have the potential to increase the oxidative stress biomarkers (TBARS, CAT activity) and MT levels; and decrease ChE activity in both gills and digestive gland. Current experimental results suggest that CAT, GST, ChE activities and MT and TBARs levels in gills and digestive gland of clam R. decussatus are sensitive and suitable responses for assessing the effects of anthropogenic contaminants on the aquatic ecosystems, particularly effluent complex mixtures., (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
49. Integrated assessment of biochemical responses in Mediterranean crab (Carcinus maenas) collected from Monastir Bay, Tunisia.
- Author
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Jebali J, Ben-Khedher S, Ghedira J, Kamel N, and Boussetta H
- Subjects
- Acetylcholinesterase metabolism, Animals, Bays, Biomarkers metabolism, Brachyura enzymology, Catalase metabolism, Gills enzymology, Glutathione Transferase metabolism, L-Lactate Dehydrogenase metabolism, Malondialdehyde metabolism, Metallothionein metabolism, Tunisia, Brachyura metabolism, Environmental Monitoring methods, Gills metabolism
- Abstract
The biochemical response of Mediteranean Crab (Carcinus maenas) collected at five stations of Monastir Bay and from Kuriat station as control was studied using a set of complementary biomarkers. The catalase, glutathione S-transferase, lactate dehydrogenase, acetycholinesterase activities; and metallothionein and malonediladehyde levels in gills were evaluated. Results revealed differences among sites in relation to each specific biomarker. Hence, a suite of biomarkers can be used to discriminate sampling sites according to types of pollution, reflecting differing conditions of anthropogenic impact. Based on Integrated Biomarker Response, the highest values and critical biochemical alteration were observed at Khniss and Ksibat in response to urban and industrial discharges and the lowest IBR value was found at reference site. The current study has shown clearly that a biomarker-based index is usefulness tool in the monitoring Tunisian coast using C. maenas as sentinel specie. Further studies in progress to investigate the seasonal variations of IBR levels and its relationship to pollutants concentrations in the sediment, gills and digestive gland of Carcinus maenas from Monastir Bay.
- Published
- 2011
- Full Text
- View/download PDF
50. Mixture toxicity assessment of nickel and chlorpyrifos in the sea bass Dicentrarchus labrax.
- Author
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Banni M, Jebali J, Guerbej H, Dondero F, Boussetta H, and Viarengo A
- Subjects
- Acetylcholinesterase metabolism, Animals, Aquaculture, Chlorpyrifos analysis, Chlorpyrifos metabolism, Glutathione Transferase metabolism, Liver drug effects, Liver enzymology, Liver metabolism, Metallothionein metabolism, NADPH-Ferrihemoprotein Reductase metabolism, Nickel analysis, Nickel metabolism, Water Pollutants, Chemical analysis, Water Pollutants, Chemical metabolism, Bass metabolism, Chlorpyrifos toxicity, Nickel toxicity, Water Pollutants, Chemical toxicity
- Abstract
The present research work was designed to study Dicentrarchus labrax biotransformation and detoxification responses to acute exposure to nickel (Ni) and chlorpyrifos (CHP). Sexually immature sea bass were treated by intraperitoneal injection of nickel chloride (500 μg kg⁻¹), chlorpyrifos (10 mg kg⁻¹), and their binary mixture for 1, 3, and 7 days. Ni and CHP accumulation was quantified in liver after the exposure periods. The following biological responses were measured: (1) NADPH cytochrome P450 reductase (NCR) activity, as phase I biotransformation parameter; (2) gluthathione S-transferase (GST) activity as a phase II conjugation enzyme, acetylcholinesterase activity, and metallothionein (MT) content. Ni bioaccumulation in the liver resulted in an increasing uptake up to 15.48 μg g⁻¹ wet weight (Ni-treated animals) and 16.73 μg g⁻¹ wet weight (mixture-treated animals) after 7 days of exposure. CHP accumulation showed a distinct pattern in animals exposed to the mixture of chemicals in comparison with CHP-treated animals. NCR activity exhibited a marked activation in CHP and mixture-treated animals. GST activity was significantly increased starting from 1 day exposure in CHP-treated animals and after 3 days in Ni-treated animals. MT accumulation increased in all conditions, with a marked synergetic effect after 7 days of exposure. These data should be carefully considered in view of the biological effects of mixture pollutants, particularly in fish farming conditions.
- Published
- 2011
- Full Text
- View/download PDF
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