1. Automated Platform for Rapid and Reproducible Sample Preparations in Point-of-Care(POC) Molecular Diagnostics
- Author
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Gyu Man Kim, Joonho Seo, Jongsu Yun, Jeawan Koo, Dongkyu Lee, Kang-Ho Lee, Ohwon Kwon, and Changwon Kim
- Subjects
Foodborne bacteria ,Chromatography ,Computer science ,010401 analytical chemistry ,Biomedical Engineering ,Bioengineering ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Molecular diagnostics ,01 natural sciences ,DNA extraction ,0104 chemical sciences ,law.invention ,Cartridge ,Fully automated ,law ,Point of care poc ,Sample preparation ,Electrical and Electronic Engineering ,0210 nano-technology ,Polymerase chain reaction ,Biotechnology - Abstract
Fully automated system for sample preparation in molecular diagnostics was developed. The small cartridge with multiple chambers and a rotating valve was designed for automated processes such as extraction, separation and purifications during the DNA sample preparation. The automated processes such as injection, mixing, capturing and valve on/off motions were controlled by an affordable, motorized control system. We demonstrated the automated DNA extraction of pathogenic bacteria using this system. The deviations in the purity and real-time polymerase chain reaction (PCR) detection of the automated DNA extraction samples were clearly decreased. The device provided rapid, reliable, and reproducible sample preparation in comparison with manual DNA extraction. This automated platform can be utilized in emergent point-of-care testing of infectious disease and foodborne bacteria.
- Published
- 2019
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