83 results on '"Jean-Paul Pillot"'
Search Results
2. Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application
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Alexandre de Saint Germain, Guillaume Clavé, Paul Schouveiler, Jean-Paul Pillot, Abhay-Veer Singh, Arnaud Chevalier, Suzanne Daignan Fornier, Ambre Guillory, Sandrine Bonhomme, Catherine Rameau, and François-Didier Boyer
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strigolactone ,profluorescent probes ,pea ,Arabidopsis thaliana ,Physcomitrium patens ,α/β-hydrolases ,Plant culture ,SB1-1110 - Abstract
Strigolactones (SLs) are intriguing phytohormones that not only regulate plant development and architecture but also interact with other organisms in the rhizosphere as root parasitic plants (Striga, Orobanche, and Phelipanche) and arbuscular mycorrhizal fungi. Starting with a pioneering work in 2003 for the isolation and identification of the SL receptor in parasitic weeds, fluorescence labeling of analogs has proven a major strategy to gain knowledge in SL perception and signaling. Here, we present novel chemical tools for understanding the SL perception based on the enzymatic properties of SL receptors. We designed different profluorescent SL Guillaume Clavé (GC) probes and performed structure-activity relationship studies on pea, Arabidopsis thaliana, and Physcomitrium (formerly Physcomitrella) patens. The binding of the GC probes to PsD14/RMS3, AtD14, and OsD14 proteins was tested. We demonstrated that coumarin-based profluorescent probes were highly bioactive and well-adapted to dissect the enzymatic properties of SL receptors in pea and a resorufin profluorescent probe in moss, contrary to the commercially available fluorescein profluorescent probe, Yoshimulactone Green (YLG). These probes offer novel opportunities for the studies of SL in various plants.
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- 2022
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3. The pea branching RMS2 gene encodes the PsAFB4/5 auxin receptor and is involved in an auxin-strigolactone regulation loop.
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Yasmine Ligerot, Alexandre de Saint Germain, Tanya Waldie, Christelle Troadec, Sylvie Citerne, Nikita Kadakia, Jean-Paul Pillot, Michael Prigge, Grégoire Aubert, Abdelhafid Bendahmane, Ottoline Leyser, Mark Estelle, Frédéric Debellé, and Catherine Rameau
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Genetics ,QH426-470 - Abstract
Strigolactones (SLs) are well known for their role in repressing shoot branching. In pea, increased transcript levels of SL biosynthesis genes are observed in stems of highly branched SL deficient (ramosus1 (rms1) and rms5) and SL response (rms3 and rms4) mutants indicative of negative feedback control. In contrast, the highly branched rms2 mutant has reduced transcript levels of SL biosynthesis genes. Grafting studies and hormone quantification led to a model where RMS2 mediates a shoot-to-root feedback signal that regulates both SL biosynthesis gene transcript levels and xylem sap levels of cytokinin exported from roots. Here we cloned RMS2 using synteny with Medicago truncatula and demonstrated that it encodes a putative auxin receptor of the AFB4/5 clade. Phenotypes similar to rms2 were found in Arabidopsis afb4/5 mutants, including increased shoot branching, low expression of SL biosynthesis genes and high auxin levels in stems. Moreover, afb4/5 and rms2 display a specific resistance to the herbicide picloram. Yeast-two-hybrid experiments supported the hypothesis that the RMS2 protein functions as an auxin receptor. SL root feeding using hydroponics repressed auxin levels in stems and down-regulated transcript levels of auxin biosynthesis genes within one hour. This auxin down-regulation was also observed in plants treated with the polar auxin transport inhibitor NPA. Together these data suggest a homeostatic feedback loop in which auxin up-regulates SL synthesis in an RMS2-dependent manner and SL down-regulates auxin synthesis in an RMS3 and RMS4-dependent manner.
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- 2017
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4. Noncanonical Strigolactone Analogues Highlight Selectivity for Stimulating Germination in Two Phelipanche ramosa Populations
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Suzanne Daignan Fornier, Alexandre de Saint Germain, Pascal Retailleau, Jean-Paul Pillot, Quentin Taulera, Lucile Andna, Laurence Miesch, Soizic Rochange, Jean-Bernard Pouvreau, and François-Didier Boyer
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Pharmacology ,Complementary and alternative medicine ,Organic Chemistry ,Drug Discovery ,Pharmaceutical Science ,Molecular Medicine ,Analytical Chemistry - Published
- 2022
5. Physcomitrium patensSMXL homologs are PpMAX2-dependent negative regulators of growth
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Ambre Guillory, Mauricio Lopez-Obando, Khalissa Bouchenine, Philippe Le Bris, Alain Lécureuil, Jean-Paul Pillot, Vincent Steinmetz, François-Didier Boyer, Catherine Rameau, Alexandre de Saint Germain, and Sandrine Bonhomme
- Abstract
SMXL proteins are a plant-specific clade of type I HSP100/Clp-ATPases.SMXLgenes are found in virtually all land plants’ genomes. However, they have mainly been studied in angiosperms. InArabidopsis thaliana, three SMXL functional subclades have been identified: SMAX1/SMXL2, SMXL345 and SMXL678. Out of these, two subclades ensure transduction on endogenous hormone signals: SMAX1/SMXL2 are involved in KAI2-ligand (KL) signaling, while SMXL678 are involved in strigolactones (SLs) signaling. Many questions remain regarding the mode of action of these proteins, as well as their ancestral role. In light of recent discoveries in the liverwortMarchantia polymorpha,we addressed this second question by investigating the function of the fourSMXLgenes of the mossPhyscomitrium patens.We demonstrate that PpSMXL proteins are negative regulators of growth, involved in the likely conserved ancestral MAX2-dependent KL signaling pathway. However, PpSMXL proteins expressed inArabidopsis thalianaunexpectedly cannot replace SMAX1/SMXL2 function in KL signaling, whereas they can functionally replace SMXL4/5 and restore root growth. Therefore, the molecular function of SMXL could be conserved, but not their interaction network. Moreover, one PpSMXL clade also positively regulates transduction of the SL signal inP. patens, this function most probably having an independent evolutionary origin to angiosperms SMXL678.
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- 2023
6. Structural and functional analyses explain Pea KAI2 receptor diversity and reveal stereoselective catalysis during signal perception
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David Cornu, François-Didier Boyer, Angelica M. Guercio, Nitzan Shabek, Alexandre de Saint Germain, Philippe Le Bris, Abdelhafid Bendahmane, Caroline Gutjahr, Salar Torabi, Catherine Rameau, Christine Le Signor, Marion Dalmais, Jean-Paul Pillot, Institut de Chimie des Substances Naturelles (ICSN), Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), University of California [Davis] (UC Davis), University of California (UC), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Agroécologie [Dijon], Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and 2047396/National Science Foundation (NSF)2028283/National Science Foundation (NSF)
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0106 biological sciences ,QH301-705.5 ,[SDV]Life Sciences [q-bio] ,Arabidopsis ,Strigolactone ,Medicine (miscellaneous) ,01 natural sciences ,General Biochemistry, Genetics and Molecular Biology ,Catalysis ,Serine ,03 medical and health sciences ,Plant Growth Regulators ,Hydrolase ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,Biology (General) ,Receptor ,Histidine ,030304 developmental biology ,Butenolide ,0303 health sciences ,[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM] ,Chemistry ,Arabidopsis Proteins ,Peas ,food and beverages ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,Ligand (biochemistry) ,Biochemistry ,Perception ,Signal transduction ,General Agricultural and Biological Sciences ,010606 plant biology & botany - Abstract
KAI2 are plant α/β hydrolase receptors, which perceive smoke-derived butenolide signals (karrikins) and putative endogenous, yet unidentified phytohormones (KAI2-ligands, KLs). The number of functional KAI2 receptors varies among plant species. It has been suggested that KAI2 gene duplication and sub-functionalization plays an adaptative role for diverse environments or ligand diversification by altering the receptor responsiveness to specific KLs. Legumes represent one of the largest families of flowering plants and contain many essential agronomic crops. Prior to legume diversification, KAI2 underwent duplication, resulting in KAI2A and KAI2B. Integrating plant genetics, ligand perception and enzymatic assays, and protein crystallography, we demonstrate that Pisum sativum KAI2A and KAI2B act as receptors and enzymes with divergent ligand stereoselectivity. KAI2B has a stronger affinity than KAI2A towards the KAI2-ligand (-)-GR24 and remarkably hydrolyses a broader range of substrates including the strigolactone-like isomer (+)-GR24. We determine the crystal structures of PsKAI2B in apo and butenolide-bound states. The biochemical and structural analyses as well as recorded mass spectra of KAI2s reveal a transient intermediate on the catalytic serine and a stable adduct on the catalytic histidine, further illuminating the role of KAI2 not only as receptors but also as bona fide enzymes. Our work uncovers the stereoselectivity of ligand perception and catalysis by evolutionarily diverged KAI2 receptors in KAR/KL signaling pathways and proposes adaptive sensitivity to KAR/KL and strigolactone phytohormones by KAI2B.
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- 2022
7. Strigolactones (SLs) modulate the plastochron by regulating KLUH (KLU) transcript abundance in Arabidopsis
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Catherine Rameau, Alexandre de Saint Germain, Jean-Bernard Pouvreau, Nicolas Arnaud, Philippe Le Bris, Jean-Paul Pillot, and Florent Cornet
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0106 biological sciences ,0303 health sciences ,Oryza sativa ,biology ,Arabidopsis Proteins ,Physiology ,Mutant ,Arabidopsis ,food and beverages ,Strigolactone ,Plant Science ,Meristem ,biology.organism_classification ,01 natural sciences ,Cell biology ,Lactones ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Arabidopsis thaliana ,Plastochron ,Plant hormone ,Heterocyclic Compounds, 3-Ring ,030304 developmental biology ,010606 plant biology & botany - Abstract
The timing of leaf emergence at the shoot apical meristem, or plastochron, is highly regulated in plants. Among the genes known to regulate the plastochron in Arabidopsis (Arabidopsis thaliana), KLUH (KLU), orthologous to the rice (Oryza sativa) PLASTOCHRON1, encodes the cytochrome P450 CYP78A5, and is thought to act through generation of a still unknown mobile signal. As klu mutants display not only a short plastochron but also a branching phenotype reminiscent of strigolactone (SL) mutants, we investigated whether KLU/CYP78A5 is involved in SL biosynthesis. We combined a genetic approach, a parasitic plant seed germination bioassay to test klu root exudates, and analysis of transcript abundances of SL-biosynthesis genes in the Arabidopsis klu mutants. We demonstrate that KLU is not involved in the SL-biosynthesis pathway. Moreover, this work allowed us to uncover a new role for SL during Arabidopsis development in modulating plastochron via a KLU-dependent pathway. Globally our data reveal that KLU is required for plastochron-specific SL responses, a first indication of crosstalk between SL and the KLU-derived signal.
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- 2021
8. Integration of the SMXL/D53 strigolactone signalling repressors in the model of shoot branching regulation in Pisum sativum
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Stephanie C. Kerr, Julie Saffar, Elizabeth A. Dun, Catherine Rameau, Jean-Paul Pillot, Suyash Bhimgonda Patil, Yannick Bellec, Christine A. Beveridge, Sylvie Citerne, Yasmine Ligerot, Alexandre de Saint Germain, and Grégoire Aubert
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0106 biological sciences ,Cytokinins ,Mutant ,Arabidopsis ,Strigolactone ,Plant Science ,01 natural sciences ,Lactones ,03 medical and health sciences ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,Loss of Function Mutation ,Auxin ,Gene expression ,Genetics ,Arabidopsis thaliana ,Gene ,Phylogeny ,Plant Proteins ,030304 developmental biology ,Feedback, Physiological ,2. Zero hunger ,chemistry.chemical_classification ,0303 health sciences ,Indoleacetic Acids ,biology ,Peas ,food and beverages ,Oryza ,Cell Biology ,Plants, Genetically Modified ,biology.organism_classification ,Cell biology ,chemistry ,Cytokinin ,Co-Repressor Proteins ,Heterocyclic Compounds, 3-Ring ,Signal Transduction ,010606 plant biology & botany - Abstract
DWARF53 (D53) in rice (Oryza sativa) and its homologs in Arabidopsis (Arabidopsis thaliana), SUPPRESSOR OF MAX2-LIKE 6 (SMXL6), SMXL7 and SMXL8, are well established negative regulators of strigolactone signalling involved in shoot branching. Little is known of pea (Pisum sativum) homologs and whether D53 and related SMXLs are specific to strigolactone signalling pathways. Here, we identify two allelic pea mutants, dormant3 (dor3), and demonstrate through gene mapping and sequencing that DOR3 corresponds to a homolog of D53 and SMXL6/SMXL7, designated PsSMXL7. Phenotype analysis, gene expression, protein and hormone quantification assays were performed to determine the role of PsSMXL7 in bud outgrowth regulation and the role of PsSMXL7 and D53 in integrating strigolactone and cytokinin responses. Like D53 and related SMXLs, we show that PsSMXL7 is strigolactone degradable, and induces feedback upregulation of PsSMXL7 transcript. Here we reveal a system conserved in pea and rice, whereby CK also upregulates PsSMXL7/D53 transcripts, providing a clear mechanism for strigolactone and cytokinin cross-talk in the regulation of branching. Further deepening our understanding of the branching network in pea, we provide evidence that strigolactone acts via PsSMXL7 to modulate auxin content via PsAFB5, which itself regulates expression of strigolactone biosynthesis genes. We therefore show that PsSMXL7 is key to a triple hormone network involving an auxin-SL feedback mechanism and SL-CK crosstalk.
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- 2021
9. Novel non-canonical strigolactone analogs highlight selectivity for stimulating germination in two Phelipanche ramosa populations
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Suzanne Daignan Fornier, Alexandre de Saint Germain, Pascal Retailleau, Jean-Paul Pillot, Quentin Taulera, Lucile Andna, Laurence Miesch, Soizic Rochange, Jean-Bernard Pouvreau, and François-Didier Boyer
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fungi ,food and beverages - Abstract
Strigolactones (SLs) are plant hormones exuded in the rhizosphere with a signaling role for the development of arbuscular mycorrhizal (AM) fungi and as stimulants of seed germination of the parasitic weeds Orobanche, Phelipanche and Striga, the most threatening weeds of major crops worldwide. Phelipanche ramosa is present mainly on rape, hemp and tobacco. P. ramosa 2a preferentially attacks hemp while P. ramosa 1 attacks rapeseed. The recently isolated Cannalactone 14 from hemp root exudates has been characterized as a non-canonical SL that selectively stimulates the germination of P. ramosa 2a seeds in comparison with P. ramosa 1. In the present work, we established that (−)-solanacol 5, a canonical orobanchol-type SL exuded by tobacco and tomato, possesses a remarkable selective germination stimulant activity for P. ramosa 2a seeds. We synthesized cannalactone analogs, named (±)-SdL19 and (±)-SdL118 which have an unsaturated acyclic carbon chain with a tertiary hydroxyl group and a methyl or a cyclopropyl group instead of a cyclohexane A-ring, respectively. (±)-SdL analogs are able to selectively stimulate P. ramosa 2a revealing that these minimal structural elements are key for this selective bioactivity. In addition, we showed that (±)-SdL19 is able to inhibit shoot branching in Pisum sativum and Arabidopsis thaliana, and induces hyphal branching in AM fungus R. irregularis, like SLs.
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- 2022
10. Methods for Phenotyping Shoot Branching and Testing Strigolactone Bioactivity for Shoot Branching in Arabidopsis and Pea
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Aitor, Muñoz, Jean-Paul, Pillot, Pilar, Cubas, and Catherine, Rameau
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Lactones ,Phenotype ,Hydroponics ,Plant Growth Regulators ,Mutation ,Arabidopsis ,Peas ,Plant Development ,Biological Assay ,Plants, Genetically Modified ,Heterocyclic Compounds, 3-Ring ,Plant Shoots - Abstract
Shoot branching is a highly variable trait that evolves during plant development and is influenced by environmental and endogenous cues such as hormones. In particular, strigolactones (SLs) are hormones that play a key role in the control of shoot branching. Branch primordia, axillary buds formed in the leaf axils, display differential growth depending on their position in the plant and also respond to hormone signaling. In this chapter, we will describe how to quantify the degree of shoot branching in two plant model species, Arabidopsis and pea, commonly used to decipher the control of this complex trait. We will also propose several methods to perform treatments of SL or SL analogs, to investigate their bioactivity and effect on the shoot branching patterns of plants of different genotypes.
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- 2021
11. Methods for Phenotyping Shoot Branching and Testing Strigolactone Bioactivity for Shoot Branching in Arabidopsis and Pea
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Aitor Muñoz, Pilar Cubas, Jean-Paul Pillot, and Catherine Rameau
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0106 biological sciences ,0301 basic medicine ,fungi ,food and beverages ,Strigolactone ,Biology ,biology.organism_classification ,01 natural sciences ,Branching (linguistics) ,03 medical and health sciences ,Plant development ,030104 developmental biology ,Arabidopsis ,Axillary bud ,Botany ,Shoot ,Primordium ,Hormone signaling ,010606 plant biology & botany - Abstract
Shoot branching is a highly variable trait that evolves during plant development and is influenced by environmental and endogenous cues such as hormones. In particular, strigolactones (SLs) are hormones that play a key role in the control of shoot branching. Branch primordia, axillary buds formed in the leaf axils, display differential growth depending on their position in the plant and also respond to hormone signaling. In this chapter, we will describe how to quantify the degree of shoot branching in two plant model species, Arabidopsis and pea, commonly used to decipher the control of this complex trait. We will also propose several methods to perform treatments of SL or SL analogs, to investigate their bioactivity and effect on the shoot branching patterns of plants of different genotypes.
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- 2021
12. Validated Method for Strigolactone Quantification by Ultra High-Performance Liquid Chromatography - Electrospray Ionisation Tandem Mass Spectrometry Using Novel Deuterium Labelled Standards
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David Touboul, Stéphanie Boutet-Mercey, François-Didier Boyer, Catherine Rameau, Amélie Roux, Grégory Mouille, Jean-Paul Pillot, François Perreau, Guillaume Clavé, and Isabelle Schmitz-Afonso
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0106 biological sciences ,0301 basic medicine ,Electrospray ,Ethyl acetate ,Strigolactone ,Plant Science ,Mass spectrometry ,Tandem mass spectrometry ,01 natural sciences ,Biochemistry ,High-performance liquid chromatography ,Analytical Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Drug Discovery ,Chromatography ,integumentary system ,Chemistry ,fungi ,Extraction (chemistry) ,General Medicine ,030104 developmental biology ,Complementary and alternative medicine ,Molecular Medicine ,Quantitative analysis (chemistry) ,010606 plant biology & botany ,Food Science - Abstract
Introduction Strigolactones (SLs) are important plant hormones. They are difficult to analyse because they occur in very small concentrations especially in comparison with other plant hormones and other substances can interfere with their detection. Objective To develop a procedure for the extraction, purification and quantification of SLs from plant roots. Methodology Samples were prepared by extraction of plant root tissues with ethyl acetate. Then the extracts were further purified with silica column chromatography. The natural SLs in the final extracts were quantified using novel deuterium labelled SLs. The results of the methodology were compared with those of the procedure of Yoneyama and coworkers. Results This procedure required about 1-g root samples to detect and quantify simultaneously the SLs (orobanchyl acetate and fabacyl acetate) concentration with high reliability. Conclusion A method was developed for determining endogenous fabacyl acetate and orobanchyl acetate in plant tissue based on novel deuterium labelled standards. A method of orobanchol quantification using a synthetic SL GR24 as internal standard was proposed. Copyright © 2017 John Wiley & Sons, Ltd.
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- 2017
13. An histidine covalent receptor and butenolide complex mediates strigolactone perception
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Frank Pelissier, Marie-Ange Badet-Denisot, Alexandre de Saint Germain, Marco Bürger, Colin G. N. Turnbull, Pascal Retailleau, Sandrine Bonhomme, David Cornu, Jean-Paul Pillot, Jean-Pierre Le Caer, Guillaume Clavé, Catherine Rameau, François-Didier Boyer, Joanne Chory, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Centre National de la Recherche Scientifique (CNRS), SICaPS (SICaPS), Département Plateforme (PF I2BC), Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay, The Salk Institute for Biological Studies, Division of cell and molecular biology, Imperial College London, Unité de Recherche en Génétique et Amélioration des Plantes (UR254), Institut National de la Recherche Agronomique (INRA), Plant Biology Laboratory and Howard Hughes Medical Institute, Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Agence Nationale de la Recherche [ANR-12-BSV6-004-01], Stream COST Action [FA1206], US National Institutes of Health [R01 GM094428], Howard Hughes Medical Institute, Catharina Foundation, Labex Saclay Plant Sciences-SPS [ANR-10-LABX-0040-SPS], Terry L. Sheppard, Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Université Sciences et Technologies - Bordeaux 1-Université Montesquieu - Bordeaux 4-Institut de Chimie du CNRS (INC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), and Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)
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0106 biological sciences ,0301 basic medicine ,Biochemistry & Molecular Biology ,Stereochemistry ,Medicinal And Biomolecular Chemistry ,Strigolactone ,Biology ,Ligands ,01 natural sciences ,Article ,Lactones ,03 medical and health sciences ,4-Butyrolactone ,Plant Growth Regulators ,Catalytic triad ,[CHIM]Chemical Sciences ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Histidine ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Biochemistry And Cell Biology ,Molecular Biology ,Plant Proteins ,Butenolide ,Molecular Structure ,Peas ,food and beverages ,Cell Biology ,biology.organism_classification ,Ligand (biochemistry) ,Karrikin ,030104 developmental biology ,Biochemistry ,Covalent bond ,Plant hormone ,Heterocyclic Compounds, 3-Ring ,010606 plant biology & botany - Abstract
International audience; Strigolactone plant hormones control plant architecture and are key players in both symbiotic and parasitic interactions. They contain an ABC tricyclic lactone connected to a butenolide group, the D ring. The DWARF14 (D14) strigolactone receptor belongs to the superfamily of α/β-hydrolases, and is known to hydrolyze the bond between the ABC lactone and the D ring. Here we characterized the binding and catalytic functions of RAMOSUS3 (RMS3), the pea (Pisum sativum) ortholog of rice (Oryza sativa) D14 strigolactone receptor. Using new profluorescent probes with strigolactone-like bioactivity, we found that RMS3 acts as a single-turnover enzyme that explains its apparent low enzymatic rate. We demonstrated the formation of a covalent RMS3-D-ring complex, essential for bioactivity, in which the D ring was attached to histidine 247 of the catalytic triad. These results reveal an undescribed mechanism of plant hormone reception in which the receptor performs an irreversible enzymatic reaction to generate its own ligand.
- Published
- 2016
14. Structure-Activity Relationship Studies of Strigolactone-Related Molecules for Branching Inhibition in Garden Pea: Molecule Design for Shoot Branching
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Catherine Rameau, Jean-Marie Beau, Jean-Paul Pillot, Arnaud Stévenin, Suzanne Ramos, Alexandre de Saint Germain, Victor Xiao Chen, Philippe Simier, Jean-Bernard Pouvreau, François-Didier Boyer, Philippe Delavault, Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut National de la Recherche Agronomique, Institut de Chimie des Substances Naturelles-Centre National de la Recherche Scientifique, and Universite de Nantes
- Subjects
0106 biological sciences ,STRIGA-GESNERIOIDES SEEDS ,Physiology ,Strigolactone ,Plant Science ,ACTS DOWNSTREAM ,Models, Biological ,01 natural sciences ,ANALOG GR24 ,ARBUSCULAR MYCORRHIZAL FUNGI ,Pisum ,Lactones ,Structure-Activity Relationship ,03 medical and health sciences ,Sativum ,Hydroponics ,BUD OUTGROWTH ,Gene Expression Regulation, Plant ,Axillary bud ,Botany ,Morphogenesis ,Genetics ,RNA, Messenger ,BIOLOGICAL EVALUATION ,Plant Proteins ,030304 developmental biology ,0303 health sciences ,Rhizosphere ,biology ,[CHIM.ORGA]Chemical Sciences/Organic chemistry ,Development and Hormone Action ,LUTEA LOUR ,Peas ,food and beverages ,Stereoisomerism ,AM FUNGI ,biology.organism_classification ,GERMINATION STIMULANTS ,Orobanche ,Germination ,Shoot ,ROOT PARASITIC PLANTS ,Biological Assay ,Oxidation-Reduction ,Plant Shoots ,010606 plant biology & botany - Abstract
Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; Initially known for their role in the rhizosphere in stimulating the seed germination of parasitic weeds such as the Striga and Orobanche species, and later as host recognition signals for arbuscular mycorrhizal fungi, strigolactones (SLs) were recently rediscovered as a new class of plant hormones involved in the control of shoot branching in plants. Herein, we report the synthesis of new SL analogs and, to our knowledge, the first study of SL structure-activity relationships for their hormonal activity in garden pea (Pisum sativum). Comparisons with their action for the germination of broomrape (Phelipanche ramosa) are also presented. The pea rms1 SL-deficient mutant was used in a SL bioassay based on axillary bud length after direct SL application on the bud. This assay was compared with an assay where SLs were fed via the roots using hydroponics and with a molecular assay in which transcript levels of BRANCHED1, the pea homolog of the maize TEOSINTE BRANCHED1 gene were quantified in axillary buds only 6 h after application of SLs. We have demonstrated that the presence of a Michael acceptor and a methylbutenolide or dimethylbutenolide motif in the same molecule is essential. It was established that the more active analog 23 with a dimethylbutenolide as the D-ring could be used to control the plant architecture without strongly favoring the germination of P. ramosa seeds. Bold numerals refer to numbers of compounds.
- Published
- 2012
15. The Pea TCP Transcription Factor PsBRC1 Acts Downstream of Strigolactones to Control Shoot Branching
- Author
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Alessandra Maia-Grondard, Abdelhafid Bendahmane, Alexandre de Saint Germain, Jean-Paul Pillot, Ioanna Antoniadi, Da Luo, Xin Li, Colin G. N. Turnbull, Stéphanie Boutet-Mercey, Christine Le Signor, Nils Braun, Marion Dalmais, Catherine Rameau, and Nathalie Bouteiller
- Subjects
0106 biological sciences ,Cytokinins ,Physiology ,Apical dominance ,Mutant ,Plant Science ,CIRCADIAN CLOCK ,01 natural sciences ,AXILLARY BUD OUTGROWTH ,Isopentenyladenosine ,Lactones ,chemistry.chemical_compound ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,Transcription (biology) ,Arabidopsis ,MUTATIONAL ANALYSIS ,Plant Proteins ,RMS1 MUTANT ,Regulation of gene expression ,0303 health sciences ,biology ,food and beverages ,Up-Regulation ,Cell biology ,MAIZE DOMESTICATION ,Biochemistry ,Cytokinin ,Life Sciences & Biomedicine ,APICAL DOMINANCE ,Plant Shoots ,Signal Transduction ,Molecular Sequence Data ,Plant Biology & Botany ,Strigolactone ,03 medical and health sciences ,Xylem ,Axillary bud ,Genetics ,FUNCTIONAL GENOMICS ,030304 developmental biology ,Science & Technology ,Arabidopsis Proteins ,Plant Sciences ,Peas ,Kinetin ,06 Biological Sciences ,biology.organism_classification ,chemistry ,Mutation ,ARABIDOPSIS-THALIANA ,07 Agricultural And Veterinary Sciences ,AUXIN TRANSPORT ,CYTOKININ CONCENTRATION ,Transcription Factors ,010606 plant biology & botany - Abstract
The function of PsBRC1, the pea (Pisum sativum) homolog of the maize (Zea mays) TEOSINTE BRANCHED1 and the Arabidopsis (Arabidopsis thaliana) BRANCHED1 (AtBRC1) genes, was investigated. The pea Psbrc1 mutant displays an increased shoot-branching phenotype, is able to synthesize strigolactone (SL), and does not respond to SL application. The level of pleiotropy of the SL-deficient ramosus1 (rms1) mutant is higher than in the Psbrc1 mutant, rms1 exhibiting a relatively dwarf phenotype and more extensive branching at upper nodes. The PsBRC1 gene is mostly expressed in the axillary bud and is transcriptionally up-regulated by direct application of the synthetic SL GR24 and down-regulated by the cytokinin (CK) 6-benzylaminopurine. The results suggest that PsBRC1 may have a role in integrating SL and CK signals and that SLs act directly within the bud to regulate its outgrowth. However, the Psbrc1 mutant responds to 6-benzylaminopurine application and decapitation by increasing axillary bud length, implicating a PsBRC1-independent component of the CK response in sustained bud growth. In contrast to other SL-related mutants, the Psbrc1 mutation does not cause a decrease in the CK zeatin riboside in the xylem sap or a strong increase in RMS1 transcript levels, suggesting that the RMS2-dependent feedback is not activated in this mutant. Surprisingly, the double rms1 Psbrc1 mutant displays a strong increase in numbers of branches at cotyledonary nodes, whereas branching at upper nodes is not significantly higher than the branching in rms1. This phenotype indicates a localized regulation of branching at these nodes specific to pea.
- Published
- 2011
16. Synthesis of Novel Haptens and Conjugates for Antibody Production against Kainoid Family
- Author
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Luc Vellutini, Marie Degueil, François-Xavier Felpin, Jean-Marie Schmitter, Jean-Paul Pillot, Etienne Baco, and Bernard Bennetau
- Subjects
Kainic acid ,Bioconjugation ,biology ,Organic Chemistry ,food and beverages ,Domoic acid ,chemistry.chemical_compound ,chemistry ,Biochemistry ,In vivo ,Amnesic shellfish poisoning ,biology.protein ,Bioassay ,Antibody ,Hapten - Abstract
Amnesic shellfish poisoning (ASP) is caused by consumption of contaminated seafood that has accumulated kainic acid or kainoid analogues such as domoic acid. Among the different ASP bioassays, immunoassays are an attractive alternative to the in vivo mouse bioassay. Herein, we report the synthesis and bioconjugation of two new haptens for the generation of a specific antibody against members of the kainoid family.
- Published
- 2010
17. Love wave biosensor for real-time detection of okadaic acid as DSP phycotoxin
- Author
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Dominique Rebière, Maria Mamani-Matsuda, Daniel Moynet, Luc Vellutini, Fabien Fournel, Bernard Bennetau, Marie Degueil, Etienne Baco, Jean-Paul Pillot, Djavad Mossalayi, Corinne Dejous, Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires (ISM), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Université Sciences et Technologies - Bordeaux 1-Université Montesquieu - Bordeaux 4-Institut de Chimie du CNRS (INC), Laboratoire d'Immunologie et Parasitologie, Université Bordeaux Segalen - Bordeaux 2, and Région Aquitaine projet n° 20071205008 FEDER opération Présage n° 30052
- Subjects
Microfluidics ,Analytical chemistry ,Peptide ,02 engineering and technology ,Biodetection ,01 natural sciences ,chemistry.chemical_compound ,Control line ,Materials Chemistry ,Electrical and Electronic Engineering ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,Instrumentation ,Engineering(all) ,chemistry.chemical_classification ,Phycotoxin ,Chromatography ,Acoustic wave ,Polydimethylsiloxane ,biology ,010401 analytical chemistry ,Metals and Alloys ,General Medicine ,Okadaic acid ,Condensed Matter Physics ,021001 nanoscience & nanotechnology ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,0104 chemical sciences ,Specific antibody ,Test line ,Love wave ,chemistry ,Microfluidic ,Polyclonal antibodies ,biology.protein ,0210 nano-technology ,Biosensor - Abstract
International audience; This paper reports the detection of okadaic acid (OA) as a Diarrheic Shellfish Poisoning (DSP) toxin with an acoustic wave platform in real-time. According to the FDA, the threshold for safe consumption of shellfish is 20 μg of OA for 100 g of shellfish tissue. The high gravimetric sensitivity of Love wave acoustic devices allow, in liquid media, immuno-detection thanks to immobilized specific antibodies. The biosensor is composed of two lines, one for the test and one used as a reference. Sensitive films were deposited through a PolyDiMethylSiloxane (PDMS) microfluidic chip. On both lines, anti-okadaic acid antibodies (OA-Ab) and saturating agents were successively injected under continuous flow with controlled flow rate. On the test line, okadaic acid (OA) was injected while an unspecific peptide (6×Histidine, 6×His) was used on the control line. On both lines, polyclonal OA-Ab were re-injected a second time to reveal previously fixed OA on the test line. Measured frequency shifts were three times higher on test lines than on control lines. In these conditions, for only 2 μg of OA used for detection, the acoustic wave platform could detect DSP toxins with only 10 g of shellfish tissue.
- Published
- 2010
- Full Text
- View/download PDF
18. The Si–B chromophore: A joint experimental and theoretical investigation
- Author
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José Carlos Araujo Da Silva, Marc Birot, Daniel Liotard, Jean-Claude Rayez, Jean-Paul Pillot, Jean-Pierre Desvergne, and Marie-Thérèse Rayez
- Subjects
Chemistry ,Organic Chemistry ,Solvatochromism ,MNDO ,Chromophore ,Photochemistry ,Biochemistry ,Inorganic Chemistry ,Dipole ,Ultraviolet visible spectroscopy ,Absorption band ,Excited state ,Materials Chemistry ,Emission spectrum ,Physical and Theoretical Chemistry - Abstract
Silylboranes with aromatic substituents linked to boron and silicon exhibit an unexpected absorption band in the UV–Vis spectral region. When polar groups were introduced, a marked solvatochromic effect was observed in their fluorescence emission spectra, revealing a strong excited state dipole moment. Semi-empirical MNDO/d and AM1 calculations showed that, upon UV excitation, the polarity of the Si–B bond increased and the aromatic π-electrons migrated toward the Si–B bond, consistent with experimental observations.
- Published
- 2008
19. Multipurpose Love acoustic wave immunosensor for bacteria, virus or proteins detection
- Author
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Duy Hai Dinh, Bernard Bennetau, Nicolas Moll, Yan Mas, Jean-Luc Lachaud, Luc Vellutini, Daniel Moynet, Jean-Paul Pillot, Emilie Pascal, Jacques Pistre, Dominique Rebière, Djavad Mossalayi, Corinne Dejous, Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), AGUR, Institut des Sciences Moléculaires (ISM), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Université Sciences et Technologies - Bordeaux 1-Université Montesquieu - Bordeaux 4-Institut de Chimie du CNRS (INC), Laboratoire d'Immunologie et Parasitologie, and Université Bordeaux Segalen - Bordeaux 2
- Subjects
Immunosensors ,Love wave ,Biomedical Engineering ,Biophysics ,Nanotechnology ,02 engineering and technology ,01 natural sciences ,Monolayer ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,ComputingMilieux_MISCELLANEOUS ,chemistry.chemical_classification ,Bacteria ,biology ,Silanization ,Biomolecule ,010401 analytical chemistry ,Acoustic wave ,021001 nanoscience & nanotechnology ,Small molecule ,0104 chemical sciences ,chemistry ,biology.protein ,Protein G ,0210 nano-technology ,Biological system ,Biosensor ,Covalent grafting - Abstract
A multipurpose Love acoustic wave biosensor is described in this article. As mass loading is one of the main effect involved in acoustic wave sensors, a great range of biomolecules could be detected using such sensors. In this way, the antibody/antigen binding property was used to immobilise the target species. We first compared different coupling agents to link the antibodies sensitive layer to the SiO2 sensor surface. Results showed that GPTS monolayer, allowing covalent attachment of antibodies bioreceptors, is better suited than DTSP and protein G. It permits to obtain a dense, stable and reproducible sensitive layer of antibodies. Then, different biological species with different size and shape like proteins, bacteriophages or bacteria were detected using such sensor. Different models have been chosen to validate the effective detection of a large species range: an anti-mouse antibody has been used to simulate small molecules (
- Published
- 2008
20. Récupération à l’autoclave de la matière organique de schistes bitumineux marocains par le phénol en conditions sub–critiques
- Author
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Mina Oumam, Marc Birot, Roger Naslain, Abdeikrim Abourriche, Jean-Paul Pillot, René Pailler, and Hassan Hannache
- Subjects
General Engineering - Abstract
La recuperation de la matiere organique des schistes bitumineux de Tarfaya a ete effectuee dans des conditions subcritiques au moyen du phenol comme solvant. Les differentes analyses effectuees sur les huiles produites ont clairement mis en evidence le role du phenol dans l'augmentation du rendement de recuperation, de la quantite des maltenes, des aromatiques et dans la reduction du taux de soufre dans les huiles des schistes bitumineux marocains.
- Published
- 2005
21. Infrared spectroscopy studies and MNDO/d calculations in organosilylborane series
- Author
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J. Araujo da Silva, Daniel Liotard, Joëlle Mascetti, J.C. Rayez, Jean-Paul Pillot, Jean-Pierre Desvergne, Marie-Thérèse Rayez, and Marc Birot
- Subjects
Inorganic Chemistry ,Symmetry element ,Chemistry ,Absorption band ,Organic Chemistry ,Analytical chemistry ,Infrared spectroscopy ,Physical chemistry ,Molecule ,MNDO ,Spectroscopy ,Analytical Chemistry - Abstract
A series of organosilylboranes has been investigated by IR spectroscopy and semiempirical calculations (MNDO/d force field methods). Computational studies afforded Si–B stretching wavenumbers in good agreement with the experimental IR bands observed in the range of 480–650 cm −1 . It was shown that these characteristic Si–B bond vibrations depend on the structure of molecules and on the size of substituting groups. Thus, compounds possessing small and medium substituting groups (H, Me) and those having a symmetry element give pure Si–B stretching bands. In the case of silylboranes bearing bulky groups, assignments of bands involving Si–B vibrators were achieved via comparison of experimental and theoretical IR spectra with those of reference molecules containing the parent organosilyl- or organoboryl moieties. An experimental absorption band near 600 cm −1 could reasonably be assigned to ν (Si–B).
- Published
- 2003
22. Regio- and stereoselective addition of sterically hindered silylboranes to terminal alkynes
- Author
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Marc Birot, Michel Pétraud, Jean-Paul Pillot, and José Carlos Araujo Da Silva
- Subjects
Steric effects ,Addition reaction ,Stereochemistry ,Organic Chemistry ,Nuclear magnetic resonance spectroscopy ,Biochemistry ,Medicinal chemistry ,Catalysis ,Inorganic Chemistry ,chemistry.chemical_compound ,Phenylacetylene ,chemistry ,Heteronuclear molecule ,Transition metal ,Materials Chemistry ,Stereoselectivity ,Physical and Theoretical Chemistry - Abstract
Novel stable organosilylboranes possessing dimesityl groups attached to boron were synthesised. They gave an addition reaction with terminal acetylenic hydrocarbons in the presence of a transition metal complex. Thus, (diphenylmethylsilyl)dimesitylborane and (diphenyl-tert-butylsilyl)dimesitylborane reacted in good yields with phenylacetylene and alk-1-ynes in the presence of Pd2(dba)3(etpo)2 as a catalyst. The structures of the products were determined by NMR spectroscopy using INEPT techniques coupled to computational simulation. Various heteronuclear coupling constants J 13 C − H and J 29 Si − H were determined for the first time in this series. The results showed that the dimesitylboryl group added to the terminal acetylenic carbon atom and the organosilyl group to the internal carbon atom, according to a regio- and stereoselective syn-addition.
- Published
- 2002
23. New strigolactone analogs as plant hormones with low activities in the rhizosphere
- Author
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Sofie Goormachtig, François-Didier Boyer, Nicolas Frei dit Frey, Jean-Paul Pillot, Catherine Rameau, Danny Geelen, Guillaume Clavé, Christian V. Stevens, Thomas S. A. Heugebaert, Amélie Roux, Jean-Bernard Pouvreau, Alexandre de Saint Germain, Stephen Depuydt, Dominique Lauressergues, Amanda Rasmussen, Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Department of Plant Production [Ghent], Faculty of Bioscience Engineering [Ghent], Universiteit Gent = Ghent University [Belgium] (UGENT)-Universiteit Gent = Ghent University [Belgium] (UGENT), Center for Plant Systems Biology (PSB Center), Vlaams Instituut voor Biotechnologie [Ghent, Belgique] (VIB), Institut National de la Recherche Agronomique (INRA), Agence Nationale de la Recherche [ANR-12-BSV6-004-01], Marie Curie International Incoming Fellowship, Newton International Fellowship, and Fund for Scientific Research Flanders (FWO Vlaanderen)
- Subjects
Hyphal growth ,Gigaspora rosea ,plant growth regulator ,Mutant ,Arabidopsis ,Strigolactone ,Plant Science ,strigolactones ,Lactones ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,root parasitic plants ,Botany ,Glomeromycota ,Molecular Biology ,Pisum sativum ,synthetic analogs ,Rhizosphere ,biology ,[CHIM.ORGA]Chemical Sciences/Organic chemistry ,fungi ,food and beverages ,biology.organism_classification ,Germination ,Plant hormone ,Weed - Abstract
International audience; : Strigolactones (SLs) are known not only as plant hormones, but also as rhizosphere signals for establishing symbiotic and parasitic interactions. The design of new specific SL analogs is a challenging goal in understanding the basic plant biology and is also useful to control plant architectures without favoring the development of parasitic plants. Two different molecules (23 (3'-methyl-GR24), 31 (thia-3'-methyl-debranone-like molecule)) already described, and a new one (AR36), for which the synthesis is presented, are biologically compared with the well-known GR24 and the recently identified CISA-1. These different structures emphasize the wide range of parts attached to the D-ring for the bioactivity as a plant hormone. These new compounds possess a common dimethylbutenolide motif but their structure varies in the ABC part of the molecules: 23 has the same ABC part as GR24, while 31 and AR36 carry, respectively, an aromatic ring and an acyclic carbon chain. Detailed information is given for the bioactivity of such derivatives in strigolactone synthesis or in perception mutant plants (pea rms1 and rms4, Arabidopsis max2 and, max4) for different hormonal functions along with their action in the rhizosphere on arbuscular mycorrhizal hyphal growth and parasitic weed germination.
- Published
- 2014
24. Strigolactones Stimulate Internode Elongation Independently of Gibberellins
- Author
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John Ross, Jean-Paul Pillot, Catherine Rameau, Yasmine Ligerot, Christine A. Beveridge, Alexandre de Saint Germain, Elizabeth A. Dun, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Sch Biol Sci, University of Queensland [Brisbane], Sch Plant Sci, Sandy Bay, University of Tasmania [Hobart, Australia] (UTAS), Ministere de l'Education Nationale, de la Recherche et de la Technologie, Australian Research Council, and University of Tasmania (UTAS)
- Subjects
0106 biological sciences ,[SDV.SA]Life Sciences [q-bio]/Agricultural sciences ,Cell division ,Physiology ,Mutant ,Arabidopsis ,Cell Count ,Plant Science ,ACTS DOWNSTREAM ,01 natural sciences ,Plant Roots ,Lactones ,Hydroponics ,Plant Proteins ,0303 health sciences ,Plant Stems ,integumentary system ,food and beverages ,Cell biology ,Phenotype ,BRANCHING SIGNALS ,Gibberellin ,PISUM-SATIVUM ,Elongation ,Plant Shoots ,Meristem ,Molecular Sequence Data ,Strigolactone ,Flowers ,Biology ,Models, Biological ,Pisum ,CELL-PROLIFERATION ,03 medical and health sciences ,GREEN-REVOLUTION ,BUD OUTGROWTH ,Axillary bud ,Botany ,Genetics ,DELLA PROTEINS ,030304 developmental biology ,Cell Size ,Cell growth ,Peas ,biology.organism_classification ,Genes, Development, and Evolution ,Gibberellins ,ROOT DEVELOPMENT ,PLANT-GROWTH ,Mutation ,Proteolysis ,ARABIDOPSIS-THALIANA ,010606 plant biology & botany - Abstract
Strigolactone (SL) mutants in diverse species show reduced stature in addition to their extensive branching. Here, we show that this dwarfism in pea (Pisum sativum) is not attributable to the strong branching of the mutants. The continuous supply of the synthetic SL GR24 via the root system using hydroponics can restore internode length of the SL-deficient rms1 mutant but not of the SL-response rms4 mutant, indicating that SLs stimulate internode elongation via RMS4. Cytological analysis of internode epidermal cells indicates that SLs control cell number but not cell length, suggesting that SL may affect stem elongation by stimulating cell division. Consequently, SLs can repress (in axillary buds) or promote (in the stem) cell division in a tissue-dependent manner. Because gibberellins (GAs) increase internode length by affecting both cell division and cell length, we tested if SLs stimulate internode elongation by affecting GA metabolism or signaling. Genetic analyses using SL-deficient and GA-deficient or DELLA-deficient double mutants, together with molecular and physiological approaches, suggest that SLs act independently from GAs to stimulate internode elongation.
- Published
- 2013
25. SYNTHESIS AND CHARACTERIZATION OF NOVEL TRIVALENT ORGANOSILYLBORANES
- Author
-
Marc Birot, Edouard Bonnefon, Jacques Dunogues, Christian Courseille, Jean-Paul Pillot, and Francis Taulelle
- Subjects
Chemistry ,Materials Chemistry ,Metals and Alloys ,General Chemistry ,Condensed Matter Physics ,Combinatorial chemistry ,QD1-999 ,Characterization (materials science) - Published
- 1996
26. Synthesis of disilylmethanes and polysilacarbosilanes, precursors of silicon carbide-based materials
- Author
-
Claude Biran, Pierre Gerval, Marc Birot, Jean-Paul Pillot, Eric Bacque, P. Lapouyade, and Jacques Dunogues
- Subjects
Magnesium ,Organic Chemistry ,Inorganic chemistry ,chemistry.chemical_element ,Biochemistry ,Carbide ,Inorganic Chemistry ,chemistry.chemical_compound ,chemistry ,Materials Chemistry ,Silicon carbide ,Physical and Theoretical Chemistry ,Tetrahydrofuran ,Dichloromethane - Abstract
Using a new procedure, substituted disilylmethanes have been prepared from chlorosilanes, dichloromethane, and magnesium in tetrahydrofuran. Bis(chlorosilyl)methanes can be used as comonomers for the synthesis of polysilacarbosilanes, which are transformed into polycarbosilanes, precursors of silicon carbide.
- Published
- 1996
27. Synthesis of new poly(2,4-disilapentane) oligomers via dehydrocondensation reaction using Group IV metallocene catalysts
- Author
-
Jean-Paul Pillot, Philippe Roux, P. Lapouyade, Jacques Dunogues, and Marc Birot
- Subjects
Zirconium ,Condensation polymer ,Silicon ,Organic Chemistry ,chemistry.chemical_element ,Biochemistry ,Catalysis ,Inorganic Chemistry ,chemistry.chemical_compound ,chemistry ,Group (periodic table) ,visual_art ,Polymer chemistry ,Materials Chemistry ,visual_art.visual_art_medium ,Organic chemistry ,Ceramic ,Physical and Theoretical Chemistry ,Metallocene ,Titanium - Abstract
Polycondensation of 2,4-disilapentane in the presence of titanium or zirconium-based catalysts afforded original poly(2,4-disilapanentane) oligomers useful as ceramic precursors.
- Published
- 1995
28. Comprehensive Chemistry of Polycarbosilanes, Polysilazanes, and Polycarbosilazanes as Precursors of Ceramics
- Author
-
Jacques Dunogues, Marc Birot, and Jean-Paul Pillot
- Subjects
Chemical engineering ,Chemistry ,visual_art ,visual_art.visual_art_medium ,General Chemistry ,Ceramic - Published
- 1995
29. New synthesis of A-ring aromatic strigolactone analogues and their evaluation as plant hormones in pea (Pisum sativum)
- Author
-
Jean-Marie Beau, Victor Xiao Chen, François-Didier Boyer, Catherine Rameau, Jean-Pierre Vors, Jean-Paul Pillot, Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Bayer CropScience [08H0120RD], Institut Universitaire de France (IUF), and Centre National de la Recherche Agronomique (INRA)
- Subjects
radical reactions ,0106 biological sciences ,STRIGA-GESNERIOIDES SEEDS ,Stereochemistry ,INHIBITION ,Strigolactone ,Metathesis ,01 natural sciences ,Radical cyclization ,Catalysis ,ARBUSCULAR MYCORRHIZAL FUNGI ,Pisum ,Kinetic resolution ,Lactones ,Structure-Activity Relationship ,Sativum ,Plant Growth Regulators ,Organic chemistry ,total synthesis ,OROBANCHE SPP ,STRUCTURAL REQUIREMENTS ,biology ,Molecular Structure ,[CHIM.ORGA]Chemical Sciences/Organic chemistry ,010405 organic chemistry ,Chemistry ,structure-activity relationships ,Organic Chemistry ,Peas ,ROOT PARASITIC WEEDS ,food and beverages ,Total synthesis ,General Chemistry ,biology.organism_classification ,GERMINATION STIMULANTS ,0104 chemical sciences ,MUTANT ,Shoot ,plant hormones ,LIPASE ,SOLANACOL ,010606 plant biology & botany - Abstract
International audience; A new general access to A-ring aromatic strigolactones, a new class of plant hormones, has been developed. The key transformations include in sequence ring-closing metathesis, enzymatic kinetic resolution and a radical cyclization with atom transfer to install the tricyclic ABC-ring system. The activity as plant hormones for the inhibition of shoot branching in pea of various analogues synthesized by this strategy is reported.
- Published
- 2012
30. Synthesis and chemical properties of 1,3-dichloro-1,3-dihydridodisilazanes
- Author
-
Marc Birot, Guy Bourgeois, Jacques Dunogues, Eric Bacque, Michel Pétraud, and Jean-Paul Pillot
- Subjects
Inorganic Chemistry ,chemistry.chemical_compound ,Chemistry ,Yield (chemistry) ,Organic Chemistry ,Materials Chemistry ,Organic chemistry ,Physical and Theoretical Chemistry ,Biochemistry ,Medicinal chemistry ,Organosilicon ,Catalysis - Abstract
A trans-silylation route to 1,3-dichloro-l,3-dihydridodisilazanes, a novel class of polyfunctional disilazane, is described. Thus, heating hexamethyl- or heptamethyl-disilazane under reflux in the presence of an excess of dichlorohydrogenosilane R 1 SiHCl 2 , led to compounds of formula (R 1 ClHSi) 2 NR 2 (R 1 = Me, Et, Vi or Ph and R 2 = H; R 1 = Me and R 2 = Me) in high yield. The exchange of chlorinated organosilicon moieties was strongly facilited by a catalytic amount of ( π Bu 4 N)F. Interpretation of the results and a few chemical properties of these novel disilazanes are reported.
- Published
- 1994
31. ChemInform Abstract: Comprehensive Chemistry of Polycarbosilanes, Polysilazanes, and Polycarbosilazanes as Precursors of Ceramics
- Author
-
Marc Birot, Jean-Paul Pillot, and Jacques Dunogues
- Subjects
Chemistry ,visual_art ,visual_art.visual_art_medium ,Nanotechnology ,General Medicine ,Ceramic - Published
- 2010
32. Novel optimized biofunctional surfaces for Love mode surface acoustic wave based immunosensors
- Author
-
Bernard Bennetau, Duy Hai Dinh, Jean-Paul Pillot, Daniel Moynet, Emilie Pascal, Colette Belin, Corinne Dejous, Dominique Rebière, Luc Vellutini, Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires (ISM), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Université Sciences et Technologies - Bordeaux 1-Université Montesquieu - Bordeaux 4-Institut de Chimie du CNRS (INC), Laboratoire d'Immunologie et Parasitologie, Université Bordeaux Segalen - Bordeaux 2, Conseil Régional d'Aquitaine, and ANR-06-ECOT-0004,ENSEIRB,Immunocapteur à ondes de Love ultra-sensible pour la détection rapide de micro-organismes dans l'eau, visant la réalisation d'un dispositif d'alerte(2006)
- Subjects
Waveguide (electromagnetism) ,Materials science ,Surface smoothing ,Nanotechnology ,02 engineering and technology ,Biofunctionalization ,010402 general chemistry ,01 natural sciences ,Antibodies ,chemistry.chemical_compound ,Monolayer ,Materials Chemistry ,Electrical and Electronic Engineering ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,Instrumentation ,Organosilicon ,Aqueous medium ,Surface acoustic wave ,Metals and Alloys ,E. coli ,[CHIM.MATE]Chemical Sciences/Material chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,SAW immunosensor ,Chemical engineering ,chemistry ,Surface modification ,0210 nano-technology ,Very-long-chain glycidyl-terminated SAM - Abstract
International audience; A very-long-chain (C22) organosilicon self-assembled monolayer (SAM) with terminal glycidyl groups was used to immobilize antibodies on the silica waveguide of a Love mode surface acoustic wave (SAW) immunosensor. This route enabled us to specifically detect, in real time, living Escherichia coli bacteria by using monoclonal anti-E. coli antibodies, leading to a marked sensor response in liquid aqueous medium. Conversely, no bacteria detection could be observed by this direct method upon functionalization of the waveguide surface by a (3-glycidoxypropyl) trimethoxysilane (GPTS) monolayer. This improvement was attributed to a surface smoothing effect resulting from the very-long-chain SAM and a better accessibility of the surface epoxide groups.
- Published
- 2010
33. Route to smooth silica-based surfaces decorated with novel self-assembled monolayers (SAMs) containing glycidyl-terminated very long hydrocarbon chains
- Author
-
Colette Belin, Luc Vellutini, Jean-Paul Pillot, Corinne Dejous, Emilie Pascal, Daniel Moynet, Bernard Desbat, Duy Hai Dinh, Dominique Rebière, Christine Labrugère, Bernard Bennetau, Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires (ISM), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Université Sciences et Technologies - Bordeaux 1-Université Montesquieu - Bordeaux 4-Institut de Chimie du CNRS (INC), Laboratoire de chimie organique et organométallique (LCOO), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), AGUR, Laboratoire d'Immunologie et Parasitologie, Université Bordeaux Segalen - Bordeaux 2, Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Université de Bordeaux (UB), Conseil Régional d'Aquitaine, and ANR-06-ECOT-0004,ENSEIRB,Immunocapteur à ondes de Love ultra-sensible pour la détection rapide de micro-organismes dans l'eau, visant la réalisation d'un dispositif d'alerte(2006)
- Subjects
Surface Properties ,Stereochemistry ,Silicon dioxide ,02 engineering and technology ,Microscopy, Atomic Force ,010402 general chemistry ,01 natural sciences ,Catalysis ,chemistry.chemical_compound ,Ellipsometry ,Cadaverine ,Spectroscopy, Fourier Transform Infrared ,Polymer chemistry ,Monolayer ,Electrochemistry ,General Materials Science ,Spectroscopy ,ComputingMilieux_MISCELLANEOUS ,Fluorescent Dyes ,Organosilicon ,Silanes ,Chemistry ,Membranes, Artificial ,Self-assembled monolayer ,Surfaces and Interfaces ,[CHIM.MATE]Chemical Sciences/Material chemistry ,Silicon Dioxide ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Fluorescence ,0104 chemical sciences ,Cross-Linking Reagents ,Self-assembly ,0210 nano-technology - Abstract
Novel glycidyl-terminated organosilicon coupling agents possessing a trialkoxysilyl head group and a very long hydrocarbon chain (C22) were synthesized. Their ability to afford densely packed self-assembled monolayers (SAMs) grafted on silica-based surfaces was investigated. Transmission FT-IR spectra showed that the most regular films were obtained by using trichloracetic acid as the catalyst (10 M%). Atomic force microscopy (AFM) and optical ellipsometry were consistent with well ordered monolayers exhibiting a marked decrease of the surface roughness. Epifluorescence microscopy revealed that these SAMs possessed a better surface reactivity than monolayers obtained with the commercially available (3-glycidoxypropyl) trimethoxysilane (GPTS) upon grafting of a fluorescent probe (dansylcadaverin). Moreover, direct attachment of fluorescent antibodies (RAG-TRITC) through covalent binding led to higher mean fluorescence intensities, showing that these new SAMs possess high potential for the immobilization of biological molecules.
- Published
- 2009
34. Strigolactone inhibition of shoot branching
- Author
-
Elizabeth A. Dun, Harro J. Bouwmeester, Philip B. Brewer, Fabien Létisse, Virginie Puech-Pagès, Soizic Rochange, Soraya Fermas, Radoslava Matusova, Christine A. Beveridge, Jean-Paul Pillot, Saida Danoun, Guillaume Bécard, Catherine Rameau, Victoria Gomez-Roldan, Jean-Charles Portais, Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT), Station Génétique d'Amélioration des Plantes (SGAP), Institut National de la Recherche Agronomique (INRA), Laboratoire de Recherche en Sciences Végétales (LRSV), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), University of Queensland [Brisbane], Evolution des Interactions Plantes-Microorganismes, Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), MetaToul Agromix, Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-MetaboHUB-MetaToul, MetaboHUB-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-MetaboHUB-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Plant Research International (PRI), Wageningen University and Research [Wageningen] (WUR), Interactions Microbiennes dans la Rhizosphère et les Racines, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, French Ministry of Research and Higher Education, ANR, The Netherlands Organisation for Scientific Research (NWO, VICI-grant), Université Fédérale Toulouse Midi-Pyrénées, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Metatoul - Agromix, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-MetaToul-MetaboHUB, Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Université Sciences et Technologies - Bordeaux 1-Université Montesquieu - Bordeaux 4-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Analyse et environnement (LAE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Centre National de la Recherche Scientifique (CNRS), and Wageningen University and Research Centre [Wageningen] (WUR)
- Subjects
0106 biological sciences ,rms1 mutant ,Parasitic plant ,Apical dominance ,[SDV]Life Sciences [q-bio] ,Mutant ,pea ,Strigolactone ,01 natural sciences ,apical dominance ,03 medical and health sciences ,Arabidopsis ,root parasitic plants ,Botany ,Laboratorium voor Plantenfysiologie ,030304 developmental biology ,germination stimulants ,0303 health sciences ,arbuscular-mycorrhizal fungi ,Multidisciplinary ,biology ,EPS-3 ,fungi ,food and beverages ,biology.organism_classification ,Cell biology ,Karrikin ,PRI Bioscience ,Orobanche ,arabidopsis ,Shoot ,seed-germination ,phosphorus deficiency ,signal ,Laboratory of Plant Physiology ,010606 plant biology & botany - Abstract
International audience; A carotenoid-derived hormonal signal that inhibits shoot branching in plants has long escaped identification. Strigolactonesare compounds thought to be derived from carotenoids and are known to trigger the germination of parasitic plant seeds andstimulate symbiotic fungi. Here we present evidence that carotenoid cleavage dioxygenase 8 shoot branching mutants of peaare strigolactone deficient and that strigolactone application restores the wild-type branching phenotype to ccd8 mutants.Moreover, we show that other branching mutants previously characterized as lacking a response to the branching inhibitionsignal also lack strigolactone response, and are not deficient in strigolactones. These responses are conserved in Arabidopsis.In agreement with the expected properties of the hormonal signal, exogenous strigolactone can be transported in shoots andact at low concentrations. We suggest that endogenous strigolactones or related compounds inhibit shoot branching inplants. Furthermore, ccd8 mutants demonstrate the diverse effects of strigolactones in shoot branching, mycorrhizalsymbiosis and parasitic weed interaction.
- Published
- 2008
35. Silicon Carbide and Carbonitride Precursors Via the Silicon-Silicon Bond Formation: Chemical and Electrochemical New Perspectives
- Author
-
Marc Birot, Françoise Spirau, Claude Biran, Michel Bordeau, Jean-Paul Pillot, and Jacques Dunogues
- Subjects
chemistry.chemical_compound ,Silicon silicon ,Chemistry ,Silicon carbide ,Nanotechnology ,Bond formation ,Electrochemistry ,Pyrolysis - Published
- 2008
36. Investigation of the pyrolysis mechanisms of cellobiose in the presence of a polysiloxane
- Author
-
Michel Pétraud, Sylvie Loison, Hervé Plaisantin, René Pailler, Marc Birot, Christine Labrugère, Pierre Olry, Patrick Simon, Gérard Daudé, Jean-Paul Pillot, Alain Guette, Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Sciences et Technologies - Bordeaux 1, Laboratoire des Composites Thermostructuraux (LCTS), Université de Bordeaux (UB)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Snecma-SAFRAN group-Centre National de la Recherche Scientifique (CNRS), Snecma Propulsion Solide (SPS), SAFRAN Group, Centre de recherches sur les matériaux à haute température (CRMHT), Centre National de la Recherche Scientifique (CNRS), Conditions Extrêmes et Matériaux : Haute Température et Irradiation (CEMHTI), Université d'Orléans (UO)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Université de Bordeaux (UB), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Sciences et Technologies - Bordeaux 1 (UB), and Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Cellobiose ,02 engineering and technology ,010402 general chemistry ,Mass spectrometry ,01 natural sciences ,Analytical Chemistry ,law.invention ,chemistry.chemical_compound ,X-ray photoelectron spectroscopy ,law ,Organic chemistry ,Cellulose ,Electron paramagnetic resonance ,Polysiloxane ,Redistributions ,[CHIM.MATE]Chemical Sciences/Material chemistry ,021001 nanoscience & nanotechnology ,Grafting ,0104 chemical sciences ,Carbon fibre ,Fuel Technology ,chemistry ,Siloxane ,0210 nano-technology ,Pyrolysis ,Nuclear chemistry - Abstract
International audience; Treatment of cellulose yarns by a polysiloxane followed by a fast pyrolysis step gave carbon fibres exhibiting strong enhancement of their mechanical resistance. To account for the beneficial effect of the polysiloxane, an investigation of the pyrolysis mechanisms was performed on a model system based on cellobiose and a polysiloxane resin. A chemical interaction between cellobiose and the resin (e.g. grafting of siloxane fragments onto the cellobiose) was established by using various physicochemical analysis techniques: nuclear magnetic resonance (NMR), photoelectron spectrometry (XPS), electronic paramagnetic resonance (EPR), Flash pyrolysis–gas chromatography–mass spectrometry (FP/GC/MS) and IR.
- Published
- 2008
37. A Love wave immunosensor for whole E. coli bacteria detection using an innovative two-step immobilisation approach
- Author
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Bernard Bennetau, Yan Mas, Djavad Mossalayi, Corinne Dejous, Duy Hai Dinh, Emilie Pascal, Dominique Rebière, Jacques Pistre, Nicolas Moll, Daniel Moynet, Jean-Paul Pillot, Import, Ims, Laboratoire de l'intégration, du matériau au système (IMS), and Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,Biomedical Engineering ,Biophysics ,Analytical chemistry ,02 engineering and technology ,Biosensing Techniques ,medicine.disease_cause ,01 natural sciences ,Mice ,Electrochemistry ,Surface roughness ,medicine ,Escherichia coli ,Animals ,Sample preparation ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,Immunoassay ,Chromatography ,biology ,Chemistry ,010401 analytical chemistry ,General Medicine ,021001 nanoscience & nanotechnology ,Grafting ,biology.organism_classification ,Enterobacteriaceae ,0104 chemical sciences ,Love wave ,0210 nano-technology ,Biosensor ,Bacteria ,Biotechnology - Abstract
The efficiency of a monomolecular film of (3-glycidoxypropyl) trimethoxysilane (GPTS) on a shear horizontal guided (Love) acoustic wave immunosensor to detect whole Escherichia coli ( E. coli ) bacteria is demonstrated. Direct anti- E. coli antibodies grafting onto the sensor surface did not lead to a significant bacteria immobilisation, partially attributed to the SiO 2 sensor surface roughness. An innovative method has been set up to get around this difficulty and to detect whole bacteria. It consists in grafting goat anti-mouse antibodies (GAM) onto the sensor surface in a first step and introducing E. coli bacteria mixed with anti- E. coli antibodies onto the sensor in a second step. We describe the characteristics of such a technique like sample preparation time (lower than 30 min) and temperature improvements. A 37 °C experimental temperature led to the fastest bacteria binding kinetic, reducing the total analysis time. This method enables to keep the specificity of the antibody/antigen interaction and provides significant results in less than 1 h. This leads to a detection threshold of 10 6 bacteria/ml in a 500 μl chamber.
- Published
- 2006
38. Enhancement of Antibody Binding on SiO2 Love Wave Sensor Surface using (3-glycidoxypropyl)trimethoxysilane
- Author
-
Corinne Dejous, Dominique Rebière, Emilie Pascal, Nicolas Moll, Jacques Pistre, Duy Hai Dinh, Daniel Moynet, Bernard Bennetau, Jean-Paul Pillot, and Yan Mas
- Subjects
Chromatography ,Materials science ,biology ,010401 analytical chemistry ,Biological membrane ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Antigen binding ,Grafting ,01 natural sciences ,Primary and secondary antibodies ,0104 chemical sciences ,3. Good health ,Love wave ,Antigen ,Biological species ,biology.protein ,Antibody ,0210 nano-technology - Abstract
An acoustic Love wave immunosensor dedicated to detect biological species such as bacteria, viruses or toxins is described in this paper. We present and analyze results of antibody grafting on the Love wave device using GPTS as coupling agent between SiO2 sensor surface and the sensitive biomembrane layer composed of antibodies. Goat anti-mouse (GAM) antibodies at concentrations from 2μg/ml to 50 μg/ml were introduced on the sensor brass cell. The saturation of the sensor surface by antibodies appears at a concentration of 20 μg/ml. The detection threshold of this primary antibody is around 2 μg/ml, and the lowest concentration allowing antigen detection is around 5 μg/ml.
- Published
- 2006
39. Grafted self-assembled monolayers derived from naturally occurring phenolic lipids
- Author
-
Colette Belin, M. Birot, Jean-Paul Pillot, T. T. T. Tran, S. Lazare, Bernard Desbat, and T. M. Dao
- Subjects
Silicon ,Hydrosilylation ,Surface Properties ,Analytical chemistry ,Molecular Conformation ,Ether ,Microscopy, Atomic Force ,Contact angle ,chemistry.chemical_compound ,Phenols ,Ellipsometry ,Monolayer ,Electrochemistry ,General Materials Science ,Spectroscopy ,Alkyl ,chemistry.chemical_classification ,Chemistry ,Water ,Self-assembled monolayer ,Stereoisomerism ,Surfaces and Interfaces ,Quartz ,Silanes ,Condensed Matter Physics ,Lipids ,Allyl Compounds ,Refractometry ,Chemical engineering ,Self-assembly ,Adsorption ,Hydrophobic and Hydrophilic Interactions ,Ethers - Abstract
Self-assembled monolayers grafted onto silicon surfaces were obtained from the hydrosilylation products by trialcoxysilanes of naturally occurring phenolic lipid allyl ethers. The as-obtained materials were characterized by various physical and physicochemical methods. Thus, contact angles of water drops showed that they possess very high hydrophobicity. Their excellent regularity was corroborated by AFM microscopy. The frequencies of the stretching CH2 infrared modes indicate the presence of alkyl chains mainly in the trans/trans conformation. Additionally, optical ellipsometry and quartz microbalance measurements enabled us to estimate the thickness of the films. The results, as a whole, are in good agreement with the formation of densely packed monolayers.
- Published
- 2005
40. Chimie douce route to novel acoustic waveguides based on biphenylene-bridged silsesquioxanes
- Author
-
Bernard Bennetau, Laurianne Blanc, Fabien Fournel, Pascal Massé, Jean-Paul Pillot, Corinne Dejous, Patrick Weisbecker, Dominique Rebière, Luc Vellutini, Michaël A. Ramin, Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Composites Thermostructuraux (LCTS), Université de Bordeaux (UB)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Snecma-SAFRAN group-Centre National de la Recherche Scientifique (CNRS), ANR-06-ECOT-0004,ENSEIRB,Immunocapteur à ondes de Love ultra-sensible pour la détection rapide de micro-organismes dans l'eau, visant la réalisation d'un dispositif d'alerte(2006), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université Sciences et Technologies - Bordeaux 1 (UB)-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Materials science ,Nanotechnology ,[CHIM.MATE]Chemical Sciences/Material chemistry ,02 engineering and technology ,General Chemistry ,Biphenylene ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Network analyzer (electrical) ,01 natural sciences ,Silsesquioxane ,0104 chemical sciences ,law.invention ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,law ,Materials Chemistry ,Insertion loss ,Surface acoustic wave sensor ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,Thin film ,0210 nano-technology ,Waveguide ,Biosensor - Abstract
International audience; Novel silsesquioxane thin films were conveniently synthesized by the sol-gel route under mild conditions. Thus, a sol was prepared from 4,4′-bis(triethoxysilyl)-1,1′-biphenyl (4,4′-BTEBp) under a hydroalcoholic medium without a surfactant, deposited by spin-coating on silica supports, and dried at 280 °C. The resulting biphenylene-bridged films exhibited very smooth surfaces, excellent mechanical behavior and no cracks. Moreover, SEM and TEM studies showed flawless, homogeneous interfaces with silica substrates. The acoustic waveguide properties of these films on piezoelectric quartz microsensors were investigated at high frequency through a network analyzer. For an optimal thickness close to 1 μm, a marked frequency shift of the main broad lobe and a decrease of the insertion loss were observed. These results show a strong effect of the waveguide for trapping the acoustic energy, promising an unprecedented ability to provide a new generation of sensors. The capability of this device in the detection of mass is also demonstrated through the immobilization of Bovine Serum Albumin (BSA). This novel approach appears very promising in view of the development of surface acoustic wave sensors and biosensors.
- Published
- 2011
41. Chimie douce route to novel acoustic waveguides based on biphenylene-bridged silsesquioxanes.
- Author
-
Pascal Massé, Luc Vellutini, Bernard Bennetau, Michaël A. Ramin, Fabien Fournel, Laurianne Blanc, Corinne Dejous, Dominique Rebière, Patrick Weisbecker, and Jean-Paul Pillot
- Abstract
Novel silsesquioxane thin films were conveniently synthesized by the sol–gel route under mild conditions. Thus, a sol was prepared from 4,4′-bis(triethoxysilyl)-1,1′-biphenyl (4,4′-BTEBp) under a hydroalcoholic medium without a surfactant, deposited by spin-coating on silica supports, and dried at 280 °C. The resulting biphenylene-bridged films exhibited very smooth surfaces, excellent mechanical behavior and no cracks. Moreover, SEM and TEM studies showed flawless, homogeneous interfaces with silica substrates. The acoustic waveguide properties of these films on piezoelectric quartz microsensors were investigated at high frequency through a network analyzer. For an optimal thickness close to 1 μm, a marked frequency shift of the main broad lobe and a decrease of the insertion loss were observed. These results show a strong effect of the waveguide for trapping the acoustic energy, promising an unprecedented ability to provide a new generation of sensors. The capability of this device in the detection of mass is also demonstrated through the immobilization of Bovine Serum Albumin (BSA). This novel approach appears very promising in view of the development of surface acoustic wave sensors and biosensors. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
42. Caracterisation par spectrometries de vibration de poly(hydrogenosilmethylenes) et de poly(chlorosilmethylenes)
- Author
-
Annette Marchand, Pierre Gerval, Jean-Paul Pillot, and Eric Bacque
- Subjects
Chain length ,symbols.namesake ,Chemistry ,Stereochemistry ,Infrared ,General Engineering ,symbols ,Physical chemistry ,Molecule ,Infrared spectroscopy ,Raman spectroscopy ,Conformational isomerism - Abstract
Infrared and Raman spectra of poly(hydrogenosilmethylenes) and of poly(chlorsosil-methylenes) are recorded and iterpreted. The results show that in the γ(SiH) region and also below 850 cm −1 where the γ(CSiC) and the γ(SiCSi) stretching vibrations are found, it is possible to distinguish the different structural units of the derivatives, and to determine the chains lengths for n = 1–5. lf n= 1, two rotamers are observed for both Si H and Si Cl molecules.
- Published
- 1988
43. New polycarbosilane models. 1. Poly[(methylchlorosilylene)methylene], a novel, functional polycarbosilane
- Author
-
Jean Paul Pillot, Eric Bacque, Jacques Dunogues, and Marc Birot
- Subjects
Inorganic Chemistry ,Gel permeation chromatography ,chemistry.chemical_compound ,Polymers and Plastics ,chemistry ,Proton ,Organic Chemistry ,Polymer chemistry ,Materials Chemistry ,Chemical modification ,Polysilane ,Organic chemistry ,Methylene - Abstract
AlCl 3 catalyse la reaction d'echange CH 3 -Cl sur le silicium du poly(dimethylsilylene) methylene(I) (obtenu a partir du tetramethyl-1,1,3,3 disila-1,3 cyclobutane)
- Published
- 1988
44. Silylation de la tetraline et obtention d'une octaline monosiliciee
- Author
-
Jacques Dunogues, Raymond Calas, Nicole Ardoin, and Jean-Paul Pillot
- Subjects
Olefin fiber ,Organic Chemistry ,chemistry.chemical_element ,Biochemistry ,Medicinal chemistry ,Inorganic Chemistry ,Solvent ,chemistry.chemical_compound ,Acetic acid ,chemistry ,Materials Chemistry ,Organic chemistry ,Lithium ,Tetralin ,Physical and Theoretical Chemistry ,Isomerization ,Tetrahydrofuran - Abstract
In the presence of lithium and in tetrahydrofuran as solvent, trimethylchlorisilane reacts with tetralin to give two new compounds, (I) and (II). The latter when refluxed with acetic acid leads to a new monosilylated olefin (III). The study of the desilylation mechanism leads to envisage an isomerization of allyl- into vinyl-silane.
- Published
- 1974
45. Synthese nouvelle d'allylcetones par voie organosilicique
- Author
-
Jean-Paul Pillot, Raymond Calas, Jacques Dunogues, Claude Biran, B. Arreguy, and F. Pisciotti
- Subjects
Inorganic Chemistry ,Allylic rearrangement ,Electrophilic substitution ,Group (periodic table) ,Chemistry ,Stereochemistry ,Organic Chemistry ,Materials Chemistry ,Lewis acids and bases ,Physical and Theoretical Chemistry ,Conjugated system ,Biochemistry - Abstract
In the presence of a Lewis acid (AlCl3, InCl3, GaCl3), acyl chlorides react with allyltrimethylsilane to give the corresponding allyl ketones, CH2CHCH2COR, in good yields. Substituted allylsilanes, synthesized by 1,4-disilylation of conjugated dienes, give a similar reaction. The electrophilic substitution of SiMe3 by a COR group occurs with allylic rearrangement and therefore silylated β,γ-ethylenic ketones, , are obtained. In most cases the allylic ketones prepared isomerize easily to the corresponding conjugated ketones; ketones having the formula CH2CHCH2COR lead uniquely to the trans-,propenylketones, MeCHCHCOR.
- Published
- 1975
46. Etude de la transformation polydimethylsilane-polycarbosilane
- Author
-
Jacques Dunogues, Eric Bacque, Jean-Paul Pillot, and Marc Birot
- Subjects
Silicon ,Chemistry ,Organic Chemistry ,Thermal decomposition ,Analytical chemistry ,chemistry.chemical_element ,Mass spectrometry ,Biochemistry ,Catalysis ,Inorganic Chemistry ,Materials Chemistry ,Physical chemistry ,Gas chromatography ,Physical and Theoretical Chemistry - Abstract
Thermolysis of linear or cyclic polydimethylsilanes into polycarbosilanes by Yajima's method has been studied by analyzing the gases evolved by means of gas chromatography and mass spectroscopy. About 20% of silicon was eliminated as methylsilanes during the reaction. Furthermore these results suggest some differences in the mechanisms of reactions with and without catalyst, as in the structures of the polycarbosilanes obtained.
- Published
- 1987
47. Sulfonation comparee de groupes divers lies au silicium. Application a la synthese d'acides sulfoniques nouveaux
- Author
-
Jacques Dunogues, Raymond Calas, Micheline Grignon-Dubois, B. Henner, Jean-Paul Pillot, and Norbert Duffaut
- Subjects
Order of reaction ,Trimethylsilyl ,Silicon ,Organic Chemistry ,Regioselectivity ,chemistry.chemical_element ,Biochemistry ,Medicinal chemistry ,Inorganic Chemistry ,chemistry.chemical_compound ,Hydrolysis ,chemistry ,Materials Chemistry ,Organic chemistry ,Physical and Theoretical Chemistry - Abstract
Sulphonation by trimethylsilyl chlorosulphonate of various substituted silicon compounds is regiospecific or highly regioselective at the silicon atom, the reaction order is found to be: allyl > α-naphthyl > phenyl > vinyl. Sulphonation of various mono- and di-silylated derivatives leads to trimethylsilylmono- and in some cases to trimethylsilyldisulphonates which by hydrolysis form new sulphonic acids. The orientation of the sulphonation is determined by the position of the silicon atom. The nature of cyclohexyl hinders sulphonation (cyclo-C 6 H 11 Si is much less reactive than MeSi), while cyclopropanyl, due to its π-character, is much more reactive.
- Published
- 1977
48. Ène-réactivité d'allylsilanes: Fonctionnalisation et régiochimie
- Author
-
Jacques Dunogues, Gérard Déléris, H. Iloughmane, Jean-Paul Pillot, Andre Laporterie, and Jacques Dubac
- Subjects
Allylic rearrangement ,Singlet oxygen ,Organic Chemistry ,chemistry.chemical_element ,Regioselectivity ,Nuclear magnetic resonance spectroscopy ,Biochemistry ,Oxygen ,Medicinal chemistry ,Inorganic Chemistry ,chemistry.chemical_compound ,chemistry ,Materials Chemistry ,Organic chemistry ,Singlet state ,Physical and Theoretical Chemistry ,Aliphatic compound ,Ene reaction - Abstract
The hydroperoxidation of various allylsilanes by singlet oxygen has been studied. The regioselectivity of this reaction compared to those of ethyl azodicarboxylate and 4-phenyl-1,2,4-triazoline-3,5-dione, is discussed. Mechanisms are considered in the general field of the ene reaction applied to allylic organometallic Group IVB compounds. The structures of the new products (alcohols, urazoles, hydrazines) have been determined, directly or after transformations, by NMR spectroscopy.
- Published
- 1985
49. Two-step synthesis of allylic silicon derivatives from enones
- Author
-
Jean-Paul Pillot, David Pandy-Szekeres, Raymond Calas, Gérard Déléris, and J. P. Picard
- Subjects
Allylic rearrangement ,Silicon ,Chemistry ,Organic Chemistry ,Drug Discovery ,Two step ,chemistry.chemical_element ,Biochemistry ,Combinatorial chemistry - Published
- 1980
50. New polycarbosilane models. 2. First synthesis of poly(silapropylene)
- Author
-
Jacques Dunogues, Marc Birot, Jean Paul Pillot, and Eric Bacque
- Subjects
Polymers and Plastics ,Proton ,Linear polymer ,Organic Chemistry ,Chemical modification ,Infrared spectroscopy ,Inorganic Chemistry ,Gel permeation chromatography ,chemistry.chemical_compound ,chemistry ,Polymer chemistry ,Materials Chemistry ,Chemical reduction ,Polysilane ,Organic chemistry ,Gas chromatography - Abstract
Polycarbosilane contenant des groupes (methyl et methyldeuteriosilylene. Reticulation terminale. Reduction du poly[(chlorosilylene)methylene]
- Published
- 1988
Catalog
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