1. Microtopographies control the development of basal protrusions in epithelial sheets
- Author
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Sarah Baïz, Lucie Perquis, Sylvie Coscoy, Benoît Rhone, Jean Octon, Vincent Semetey, Qingzong Tseng, François Amblard, Laboratoire Physico-Chimie Curie [Institut Curie] (PCC), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Ligue Nationale Contre le Cancer - Paris, Ligue Nationnale Contre le Cancer, Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL), Laboratoire Procédés et Ingénierie en Mécanique et Matériaux (PIMM), Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM), and Ulsan National Institute of Science and Technology (UNIST)
- Subjects
0301 basic medicine ,Scaffold ,Materials science ,Surface Properties ,General Physics and Astronomy ,Context (language use) ,02 engineering and technology ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Sciences de l'ingénieur ,General Biochemistry, Genetics and Molecular Biology ,Madin Darby Canine Kidney Cells ,Biomaterials ,03 medical and health sciences ,chemistry.chemical_compound ,Dogs ,Imaging, Three-Dimensional ,Tissue engineering ,Cell Adhesion ,medicine ,Animals ,General Materials Science ,Microscopy, Confocal ,Cell Membrane ,Epithelial Cells ,General Chemistry ,Adhesion ,021001 nanoscience & nanotechnology ,Epithelium ,030104 developmental biology ,Membrane ,medicine.anatomical_structure ,Microscopy, Fluorescence ,chemistry ,Biophysics ,Cell Surface Extensions ,Adhesive ,0210 nano-technology ,Ethylene glycol - Abstract
International audience; Cells are able to develop various types of membrane protrusions that modulate their adhesive, migratory, or functional properties. However, their ability to form basal protrusions, particularly in the context of epithelial sheets, is not widely characterized. The authors built hexagonal lattices to probe systematically the microtopography-induced formation of epithelial cell protrusions. Lattices of hexagons of various sizes (from 1.5 to 19 μm) and 5–10 μm height were generated by two-photon photopolymerization in NOA61 or poly(ethylene glycol) diacrylate derivatives. The authors found that cells generated numerous, extensive, and deep basal protrusions for hexagons inferior to cell size (3–10 μm) while maintaining a continuous epithelial layer above structures. They characterized the kinetics of protrusion formation depending on scaffold geometry and size. The reported formation of extensive protrusions in 3D microtopography could be beneficial to develop new biomaterials with increased adhesive properties or to improve tissue engineering.
- Published
- 2018
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