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The exhalation of aerosols during musical performances or rehearsals posed a risk of airborne virus transmission in the COVID-19 pandemic. Previous research studied aerosol plumes by only focusing on one risk factor, either the source strength or convective transport capability. Furthermore, the source strength was characterized by the aerosol concentration and ignored the airflow rate needed for risk analysis in actual musical performances. This study characterizes aerosol plumes that account for both the source strength and convective transport capability by conducting experiments with 18 human subjects. The source strength was characterized by the source aerosol emission rate, defined as the source aerosol concentration multiplied by the source airflow rate (brass 383 particle/s, singing 408 particle/s, and woodwind 480 particle/s). The convective transport capability was characterized by the plume influence distance, defined as the sum of the horizontal jet length and horizontal instrument length (brass 0.6 m, singing 0.6 m and woodwind 0.8 m). Results indicate that woodwind instruments produced the highest risk with approximately 20% higher source aerosol emission rates and 30% higher plume influence distances compared with the average of the same risk indicators for singing and brass instruments. Interestingly, the clarinet performance produced moderate source aerosol concentrations at the instrument's bell, but had the highest source aerosol emission rates due to high source airflow rates. Flute performance generated plumes with the lowest source aerosol emission rates but the highest plume influence distances due to the highest source airflow rate. Notably, these comprehensive results show that the source airflow is a critical component of the risk of airborne disease transmission. The effectiveness of masking and bell covering in reducing aerosol transmission is due to the mitigation of both source aerosol concentrations and plume influence distances. This study also found a musician who generated approximately five times more source aerosol concentrations than those of the other musicians who played the same instrument. Despite voice and brass instruments producing measurably lower average risk, it is possible to have an individual musician produce aerosol plumes with high source strength, resulting in enhanced transmission risk; however, our sample size was too small to make generalizable conclusions regarding the broad musician population.

Researchers have established improved methods for undergraduate science and engineering education, yet these efforts often overlook the personal meaning students find in their work. Institutions of higher learning should support the creation of personal meaning along with content mastery, aspects that are both included in arts education. The authors argue that STEM educators must work to overcome student perception that content mastery and personal meaning sit at odds. The authors provide an example of a technical course that achieves these goals as well as evidence that it is possible to foster connection while developing content mastery.

The COVID-19 pandemic has created a great deal of fear and uncertainty around whether aerosols from singers and musical instruments can transmit the virus. The World Health Organization has recognized that SARS-COV-2 is transmissible through aerosols. Outbreaks from choir performances, such as the Skagit Valley Choir, showed that singing brings potential risk of COVID-19 infection. Additionally, outbreaks from singing have resulted in superspreader events leading to many infections and deaths. There is less known about the risks of airborne infection from other musical performance, such as playing wind instruments or performing theater. Hence, aerosol generation in performance (playing brass and woodwind instruments, singing, and theater speech delivery) should be quantified, monitored, and mitigated. To tackle this issue our research questions were as follows: (i) What is the aerosol generation rate? (ii) How does air flow from the performer’s mouth/instrument and how does it disseminate into the environment? and (iii) What control methods can be employed to mitigate risk? In this study, we used a variety of methods, including flow visualization, aerosol and CO2 measurements, and computational fluid dynamics (CFD) modeling to understand the different components that can lead to transmission risk from musical performance and risk mitigation.

This paper (Part B) presents the results of sensitivity, baseline performance, and levelized cost of energy analyses of a recently proposed Pumped Thermal Energy Storage and Bottoming System (Bot-PTES) that uses ammonia as the working fluid. The system model was outlined in Part A of this two-part paper. This analysis focuses on the effects of hot thermal storage utilization, system pressure, and evaporator/condenser size on the system performance. It also presents the estimated performance for a proposed baseline Bot-PTES. Results of this analysis showed that all selected parameters had significant effects on efficiency, with the evaporator/condenser size having the largest effect over the selected ranges. Results for the baseline case showed stand-alone energy storage efficiencies between 51 and 66% for varying power levels and charge states, and a stand-alone bottoming efficiency of 24%. The resulting efficiencies for this case were low compared to competing technologies; however, the dual-functionality of the Bot-PTES enables it to have higher capacity factor, leading to $91–197/MWh levelized cost of energy compared to $262–284/MWh for batteries and $172–254/MWh for Compressed Air Energy Storage.

Purpose To develop an estimate of pulmonary vascular resistance (PVR) using blood flow measurements from 3D velocity-encoded phase contract magnetic resonance imaging (here termed 4D MRI). Materials and Methods In all, 17 patients with pulmonary hypertension (PH) and five controls underwent right heart catheterization (RHC), 4D and 2D Cine MRI (1.5T) within 24 hours. MRI was used to compute maximum spatial peak systolic vorticity in the main pulmonary artery (MPA) and right pulmonary artery (RPA), cardiac output, and relative area change in the MPA. These parameters were combined in a four-parameter multivariate linear regression model to arrive at an estimate of PVR. Agreement between model predicted and measured PVR was also evaluated using Bland–Altman plots. Finally, model accuracy was tested by randomly withholding a patient from regression analysis and using them to validate the multivariate equation. Results A decrease in vorticity in the MPA and RPA were correlated with an increase in PVR (MPA: R2 = 0.54, P < 0.05; RPA: R2 = 0.75, P < 0.05). Expanding on this finding, we identified a multivariate regression equation that accurately estimates PVR (R2 = 0.94, P < 0.05) across severe PH and normotensive populations. Bland–Altman plots showed 95% of the differences between predicted and measured PVR to lie within 1.49 Wood units. Model accuracy testing revealed a prediction error of ∼20%. Conclusion A multivariate model that includes MPA relative area change and flow characteristics, measured using 4D and 2D Cine MRI, offers a promising technique for noninvasively estimating PVR in PH patients. J. MAGN. RESON. IMAGING 2016;44:914–922.

In this study, a commercial electromagnetic synthetic jet actuator (SJA) was characterized to determine its flow patterns and sphere of influence within a room. Its axisymmetry was also evaluated. The objective of this research is to explore the potential application of this commercial SJA for indoor air quality (IAQ) control and its limitations for such application. A small fan of similar size was also partially characterized. Results showed that the SJA is axisymmetric, and it uses a small power input to impact the airflow over 1 m from the jet exit. Further, while the SJA showed less flow output than the fan in this study, it had significantly greater dynamic pressure per unit power. This feature could be potentially useful for indoor air quality control applications.

Diastolic dysfunction, a leading cause of heart failure in the US, is a complex pathology which manifests morphological and hemodynamic changes in the heart and circulatory system. Recent advances in time-resolved phase-contrast cardiac magnetic resonance imaging (4D Flow) have allowed for characterization of blood flow in the right ventricle (RV) and right atrium (RA), including calculation of vorticity and qualitative visual assessment of coherent flow patterns. We hypothesize that right ventricular diastolic dysfunction (RVDD) is associated with changes in vorticity and right heart blood flow. This paper presents background on RVDD, and 4D Flow tools and techniques used for quantitative and qualitative analysis of cardiac flows in the normal and disease states. In this study, 20 patients with RVDD and 14 controls underwent cardiac 4D Flow and echocardiography. A method for determining the time-step for peak early diastole using 4D Flow data is described. Spatially integrated early diastolic vorticity was extracted from the RV, RA, and combined RV/RA regions of each subject using a range of vorticity thresholding and scaling methods. Statistically significant differences in vorticity were found in the RA and combined RA/RV in RVDD subjects compared to controls when vorticity vectors were both thresholded and scaled by cardiac index.

Room air distribution in hospital operating rooms (OR) is critical to the effective functioning of surgical procedures, but the air distribution patterns are governed by complex physics that are currently not well understood. Both qualitative and quantitative flow visualization techniques were used to evaluate the room air distribution in a full-scale chamber designed to simulate a hospital operating room. A laser sheet illumination technique was used to identify key features of the room air distribution, and particle image velocimetry (PIV) was used to measure the velocity field in a plane crossing the surgical site. Hospital operating rooms require the use of ASHRAE Group E diffusers in an array above the surgical table, providing downward, unidirectional, non-aspirating air flow across the sterile region of the room. The supply air jet is characterized by complex physics, including annular shape, impingement, buoyancy, a large jet to room aspect ratio, and recirculation. The large diameter of the jet relative to the room size makes the overall room air distribution highly sensitive to the parameters of the supply air. The air distribution pattern in the room was found to have relatively low velocity and turbulence near the supply air diffuser, but increasing velocity and turbulence in the shear region at the edge of the supply air jet. Flow visualization and PIV methods both demonstrated an angle of the shear layer inwards towards the center of the jet. This flow feature reduces the overall coverage area for the sterile air flow and may pose a risk to the protection of the surgical patient.

Measurement of hemodynamic wall shear stress (WSS) is important in investigating the role of WSS in the initiation and progression of atherosclerosis. Echo particle image velocimetry (echo PIV) is a novel ultrasound-based technique for measuring WSS in vivo that has previously been validated in vitro using the standard optical PIV technique. We evaluated the repeatability and reproducibility of echo PIV for measuring WSS in the human common carotid artery. We measured WSS in 28 healthy participants (18 males and 10 females, mean age: 56 ± 12 y). Echo PIV was highly repeatable, with an intra-observer variability of 1.0 ± 0.1 dyn/cm2 for peak systolic (maximum), 0.9 dyn/cm2 for mean and 0.5 dyn/cm2 for end-diastolic (minimum) WSS measurements. Likewise, echo PIV was reproducible, with a low inter-observer variability (max: 2.0 ± 0.2 dyn/cm2, mean: 1.3 ± 0.1 dyn/cm2, end-diastolic: 0.7 dyn/cm2) and more variable inter-scan (test–retest) variability (max: 7.1 ± 2.3 dyn/cm2, mean: 2.9 ± 0.4 dyn/cm2, min: 1.5 ± 0.1 dyn/cm2). We compared echo PIV with the reference method, phase-contrast magnetic resonance imaging (PC-MRI); echo PIV-based WSS measurements agreed qualitatively with PC-MRI measurements (r = 0.89, p 0.05). For the first time, we report that echo PIV can measure WSS with good repeatability and reproducibility in adult humans with a broad age range. Echo PIV is feasible in humans and offers an easy-to-use, ultrasound-based, quantitative technique for measuring WSS in vivo in humans with good repeatability and reproducibility.

Our objective was to determine whether left ventricular (LV) vorticity (ω), the local spinning motion of a fluid element, correlated with markers of ventricular interdependency in pulmonary hypertension (PH). Maladaptive ventricular interdependency is associated with interventricular septal shift, impaired LV performance, and poor outcomes in PH patients, yet the pathophysiologic mechanisms underlying fluid-structure interactions in ventricular interdependency are incompletely understood. Because conformational changes in chamber geometry affect blood flow formations and dynamics, LV ω may be a marker of LV-RV (right ventricular) interactions in PH. Echocardiography was performed for 13 PH patients and 10 controls for assessment of interdependency markers, including eccentricity index (EI), and biventricular diastolic dysfunction, including mitral valve (MV) and tricuspid valve (TV) early and late velocities (E and A, respectively) as well as MV septal and lateral early tissue Doppler velocities (e'). Same-day 4-dimensional cardiac magnetic resonance was performed for LV E (early)-wave ω measurement. LV E-wave ω was significantly decreased in PH patients (P = 0.008) and correlated with diastolic EI (Rho = -0.53, P = 0.009) as well as with markers of LV diastolic dysfunction, including MV E(Rho = 0.53, P = 0.011), E/A (Rho = 0.56, P = 0.007), septal e' (Rho = 0.63, P = 0.001), and lateral e' (Rho = 0.57, P = 0.007). Furthermore, LV E-wave ω was associated with indices of RV diastolic dysfunction, including TV e' (Rho = 0.52, P = 0.012) and TV E/A (Rho = 0.53, P = 0.009). LV E-wave ω is decreased in PH and correlated with multiple echocardiographic markers of ventricular interdependency. LV ω may be a novel marker for fluid-tissue biomechanical interactions in LV-RV interdependency.

Cough generated infectious aerosols are of interest while developing strategies for the mitigation of disease risks ranging from the common cold to SARS. In this work, the velocity field of human cough was measured using particle image velocimetry (PIV). The project subjects (total 29) coughed into an enclosure seeded with stage fog. Cough flow velocity profiles, average widths of the cough jet, and maximum cough velocities were measured. Maximum cough velocities ranged from 1.5 m/s to 28.8 m/s. The average width of all coughs ranged between 35 to 45 mm. Wide variability in the data suggests that future cough simulations consider a range of conditions.

In data assimilation, information from sensors is used to correct the state variables of a numerical model. This has been used to great advantage by the weather prediction community in the context of direct numerical simulation (DNS) models, but has seen comparatively little use in point-vortex models. This is due in large part to data- processing issues. In order to keep up with the speeds necessary for effective data assimilation, one must extract and discretize the vortex structures from velocity field data in a computationally efficient fashion—i.e., using as few discrete vortices as possible to model the measured flow. This paper describes a new strategy for accomplishing this and evaluates the results using data from a laboratory-scale vortex-dominated planar jet. Large-scale vortex structures are found using a family of variants on traditional vortex extraction methods. By augmenting these methods with simple computational topology techniques, one obtains a new method that finds the boundaries of the coherent structures in a manner that naturally follows the geometry of the flow. This strategy was evaluated in the context of two standard vortex extraction methods, vorticity thres- holding and Okubo-Weiss, and tested upon velocity field data from the experimental fluid flow. The large-scale structures found in this manner were then modeled with collections of discrete vortices, and the effects of the grain size of the discretization and the parameters of the discrete vortex model were studied. The results were evaluated by comparing the instantaneous velocity field induced by the discrete vortices to that measured in the jet. These com- parisons showed that the two extraction techniques were comparable in terms of sensitivity and error, suggesting that the computationally simpler vorticity thresholding method is more appropriate for applications where speed is an issue, like data assimilation. Comparisons of different discretization strategies showed that modeling each large- scale vortex structure with a single discrete vortex provided the best compromise between mean-squared error and computational effort. These results are of potential interest in any situation where one must balance accuracy and expense while extracting vortices from a snapshot of a flow field; data assimilation is only one example.

A stereomicroscopic particle image velocimetry (SμPIV) system has been developed for millimeter scale flows. The SμPIV system is based on an off-the-shelf stereomicroscope, with magnification between 0.69× and 30×, and a field of view between 7.5 × 6 mm and 250 × 200 μm. Custom calibration targets were devised using printed circuit board technology, and applied at a magnification factor of 1.74, with a field of view of 4.75 × 3.8 mm. Measurement errors were assessed by moving a test block with fixed particles. Total system uncertainty in test block displacement transverse to the optical axis was 0.5% of the field of view, and 3% of the depth of field for motion along the optical axis. Approximately 20% of this uncertainty was due to the calibration target quality and test block procedures.

We have recently developed an ultrasound-based velocimetry technique, termed echo particle image velocimetry (Echo PIV), to measure multi-component velocity vectors and local shear rates in arteries and opaque fluid flows by identifying and tracking flow tracers (ultrasound contrast microbubbles) within these flow fields. The original system was implemented on images obtained from a commercial echocardiography scanner. Although promising, this system was limited in spatial resolution and measurable velocity range. In this work, we propose standard rules for characterizing Echo PIV performance and report on a custom-designed Echo PIV system with increased spatial resolution and measurable velocity range. Then we employed this system for initial measurements on tube flows, rotating flows and in vitro carotid artery and abdominal aortic aneurysm (AAA) models to acquire the local velocity and shear rate distributions in these flow fields. The experimental results verified the accuracy of this technique and indicated the promise of the custom Echo PIV system in capturing complex flow fields non-invasively.

This paper describes recent progress in flow visualization techniques from the viewpoint of visual art incorporating fluid motion. The images of fluid art introduced here are categorized into four groups: the reflected or refracted patterns of free surface motion in nature and in a controlled environment, the coherent turbulent phenomena of fluid flow, and the fluid motion induced by the physical properties of fluids. It is shown that flow visualization techniques, which were originally developed in the field of engineering, have been successfully applied to the creation of artistic images.

To develop an estimate of pulmonary vascular resistance (PVR) using blood flow measurements from 3D velocity-encoded phase contract magnetic resonance imaging (here termed 4D MRI).In all, 17 patients with pulmonary hypertension (PH) and five controls underwent right heart catheterization (RHC), 4D and 2D Cine MRI (1.5T) within 24 hours. MRI was used to compute maximum spatial peak systolic vorticity in the main pulmonary artery (MPA) and right pulmonary artery (RPA), cardiac output, and relative area change in the MPA. These parameters were combined in a four-parameter multivariate linear regression model to arrive at an estimate of PVR. Agreement between model predicted and measured PVR was also evaluated using Bland-Altman plots. Finally, model accuracy was tested by randomly withholding a patient from regression analysis and using them to validate the multivariate equation.A decrease in vorticity in the MPA and RPA were correlated with an increase in PVR (MPA: R(2) = 0.54, P0.05; RPA: R(2) = 0.75, P0.05). Expanding on this finding, we identified a multivariate regression equation that accurately estimates PVR (R(2) = 0.94, P0.05) across severe PH and normotensive populations. Bland-Altman plots showed 95% of the differences between predicted and measured PVR to lie within 1.49 Wood units. Model accuracy testing revealed a prediction error of ∼20%.A multivariate model that includes MPA relative area change and flow characteristics, measured using 4D and 2D Cine MRI, offers a promising technique for noninvasively estimating PVR in PH patients. J. MAGN. RESON. IMAGING 2016;44:914-922.

Flow Visualization is both the practice of making fluid physics visible and a course that focuses on the practice of making those images, as short video and stills. Since 2003, this course has been cross-listed as a mechanical engineering elective and a fine arts studio course, and brings together mixed teams of engineering and fine arts photography or film students. With its focus on the production of aesthetically pleasing and scientifically useful images of fluid flows, it is one of the few course offerings that explicitly calls upon engineering students to use an aesthetic sense in their studies. In prior work, this course was associated with a positive shift in affect toward fluid flows, which we measured through the Fluids Perception Survey (FluPerS) [1]. In the same survey, students expressed a greater awareness of fluids. This outcome was in contrast to the survey results from Fluid Mechanics, a traditional engineering core course, with a highly analytic, mathematical approach. Exit surveys of students in Fluid Mechanics reveal a negative shift in affect, which is typical of other technical courses, with no comments about awareness of fluids. The increased awareness of fluids can be termed an expansion of perception — when learners see everyday objects, events, or issues through the lens of the content [2]. Expansion of perception is often associated with deeper conceptual understanding and the ability to transfer learning to new settings. Building on the prior survey work, we conducted interviews with students from Flow Vis, both engineering students (n=4) and art/film students (n=5), in addition to Fluid Mechanics students (n=3), at the beginning and end of a semester. Their comments, along with open responses in the survey (n=86), are being analyzed using an iterative process to develop an emergent coding scheme. We want to discover what about Flow Vis helps students, both from engineering and art, internalize and apply their understanding of fluids. What role does the emphasis on aesthetics, so familiar to the art students, so uncommon in coursework for the engineers, play? Finally, how can we adapt the teaching practices from Flow Vis for other courses, other content areas? Analysis of data and future course suggestions are discussed.

This special session takes the form of a guided discussion, using a hands-on flow visualization exercise as focal point. Our goals are 1) to foster conversation and document ideas about how the aesthetic qualities of engineering topics can be used to deliberately draw the emotional engagement of students; 2) to gauge how the FIE community currently views the aesthetics of engineering, and brainstorm new visions for how aesthetics could be used to improve recruitment and retention of a diverse student population as well as lead to innovative methods for the teaching and learning of core engineering content; 3) to explore the feasibility of viewing aesthetics-driven emotional engagement as a necessity and not an ancillary benefit in course design.

Right ventricular diastolic dysfunction (RVDD) is an important prognostic indicator in pulmonary arterial hypertension (PAH). RV vortex rings have been observed in healthy subjects, but their significance in RVDD is unknown. Vorticity, the local spinning motion of an element of fluid, may be a sensitive measure of RV vortex dynamics. Using four-dimensional (4D) flow cardiac magnetic resonance imaging (CMR), we investigated the relationship between right heart vorticity with echocardiographic indexes of RVDD. Thirteen (13) PAH subjects and 10 controls underwent same-day 4D flow CMR and echocardiography. RV diastolic function was assessed using trans-tricuspid valve (TV) early (E) and late (A) velocities, E/A ratio, and e' and a' tissue Doppler velocities. RV and right atrial (RA) integrated mean vorticity was calculated for E and A-wave filling periods using 4D datasets. Compared with controls, A-wave vorticity was significantly increased in RVDD subjects in both the RV [2343 (1,559–3,295) vs. 492 (267-2,649) 1/s, P = 0.028] and RA [30 (27–44) vs. 9 (5–27) 1/s, P = 0.005]. RA E vorticity was significantly decreased [13 (7–22) vs. 28 (15–31) 1/s, P = 0.038] in RVDD. E-wave vorticity correlated TV e', E-,and TV E/A ( P < 0.05), and A-wave vorticity associated with both TV A and E/A ( P < 0.02). RVDD is associated with alterations in E- and A-wave vorticity, and vorticity correlates with multiple echocardiographic markers of RVDD. Vorticity may be a robust noninvasive research tool for the investigation of RV fluid and tissue mechanical interactions in PAH.

The Fontan operation, an efficient palliative surgery, is performed for patients with single-ventricle pathologies. The total cavopulmonary connection is a preferred Fontan procedure in which the superior and inferior vena cava are connected to the left and right pulmonary artery. The overall goal of this work is to develop an artificial right ventricle that can be introduced into the inferior vena cava, which would act to reverse the deleterious hemodynamics in post-Fontan patients. We present the initial design and computational analysis of a micro-axial pump, designed with the particular hemodynamics of Fontan physiology in mind. Preliminary in vitro data on a prototype pump are also presented. Computational studies showed that the new design can deliver a variety of advantageous operating conditions, including decreased venous pressure through proximal suction, increased pressure rise across the pump, increased pulmonary flows, and minimal changes in superior vena cava pressures. In vitro studies on a scaled prototype showed trends similar to those seen computationally. We conclude that a micro-axial flow pump can be designed to operate efficiently within the low-pressure, low-flow environment of cavopulmonary flows. The results provide encouragement to pursue this design to for in vitro studies and animal studies.

Experiments studying cardiovascular geometries require a working fluid that matches the high index of refraction of glass and silicone, has a low viscosity, and is safe and inexpensive. A good candidate working fluid is diethyl phthalate (DEP), diluted with ethanol. Measurements were made of index of refraction and viscosity of varied dilutions at a range of temperatures, and empirical models are proposed. Material compatibility tests showed that only specific formulations of ABS, acrylic, vinyl and PVC are compatible. A silicone elastomer additionally tested negative for change in compliance with DEP exposure.

In Spring 2003, a new experimental course on flow visualization was offered to a mixed class of Fine Arts Photography and Engineering students. Course content included fluid flow physics, history of photography with respect to the relationship of science and art, as well as flow visualization and photography techniques. Issues such as "What makes an image art? What makes an image scientific?" were addressed. The class focused on studio/laboratory experiences for mixed teams of students. In Spring 2004 these concepts were distilled into an engineering outreach experience for middle school girls. The spectacular images resulting from these experiments show that flow visualization can be both performed and appreciated by a broad spectrum of people. Thus flow visualization may represent a new bridge between scientists and non-scientists.

Accurate measurement of velocity profiles, multiple velocity vectors and local shear stress in arteries is very important for a variety of cardiovascular diseases. We have recently developed an ultrasound based velocimetry technique, termed echo particle image velocimetry (echo PIV). This method takes advantage of the nonlinear backscatter characteristics of ultrasound contrast microbubbles when exposed to certain ultrasonic forcing conditions. Preliminary in vitro, animal and clinical studies have shown significant promise of this method for measuring multiple velocity components with good temporal (up to 2 ms) and spatial (

Background— Current evaluation of pulmonary hypertension (PH) in children involves measurement of pulmonary vascular resistance (PVR); however, PVR neglects important pulsatile components. Pulmonary artery (PA) input impedance and ventricular power (VP) include mean and pulsatile effects and have shown promise as alternative measures of vascular function. Here we report the utility of pulsed-wave (PW) Doppler-measured instantaneous flow and pressure measurements for estimation of input impedance and VP and use this method to develop a novel parameter: reactivity in compliance. Methods and Results— An in vitro model of the general pulmonary vasculature was used to obtain impedance and VP, measured by PW Doppler and a reference flow meter. The method was then tested in a preliminary clinical study in subjects with normal PA hemodynamics (n=4) and patients with PH undergoing reactivity evaluation (8 patients; 23 data points). In vitro results showed good agreement between the impedance spectra computed from both flow-measurement methods. Excellent correlation was seen in vitro between actual resistance and the zero-frequency (Z o ) impedance value ( r 2 =0.984). Excellent agreement was also found between Z o and PVR in the clinical measurements ( y =1.075 x +0.73; r =0.993). Furthermore, total VP and VP/cardiac output increased significantly with hypertension (128.73 to 365.91 mW and 2.42 to 6.69 mW · mL −1 · s −1 , respectively). The first-harmonic value of impedance (Z 1 ) was used as a measure of compliance reactivity; older patients exhibited markedly less compliance reactivity than did younger patients. Conclusions— Input impedance and VP calculated from Doppler measurements and a single-catheter pressure measurement provide comprehensive characterization of PH and reactivity.

Although accurate measurement of velocity profiles, multiple velocity vectors, and shear stress in arteries is important, there is still no easy method to obtain such information in vivo. We report on the utility of combining ultrasound contrast imaging with particle image velocimetry (PIV) for noninvasive measurement of velocity vectors. This method (echo PIV) takes advantage of the strong backscatter characteristics of small gas-filled microbubbles (contrast) seeded into the flow. The method was tested in vitro. The steady flow analytical solution and optical PIV measurements (for pulsatile flow) were used for comparison. When compared to the analytical solution, both echo PIV and optical PIV resolved the steady velocity profile well. Error in shear rate as measured by echo PIV (8%) was comparable to the error of optical PIV (6.5%). In pulsatile flow, echo PIV velocity profiles agreed well with optical PIV profiles. Echo PIV followed the general profile of pulsatile shear stress across the artery but underestimated wall shear at certain time points. However, error in shear from echo PIV was an order of magnitude less than error from current shear measurement methods. These studies indicate that echo PIV is a promising technique for noninvasive measurement of velocity profiles and shear stress.

We have developed a promising non-invasive ultrasound-based method for performing particle image velocimetry (PIV) in vivo. This method, termed echo PIV, provides multi-component blood velocity data with good ( approximately 2 ms) temporal resolution. The method takes advantage of the non-linear ultrasound backscatter characteristics of small gas-filled microbubbles (ultrasound contrast) that are seeded into the blood stream. In this study, we use a numerical model to explore potential areas to focus future work in echo PIV. Ultrasound backscatter from encapsulated microbubbles was modeled using a modified Rayleigh-Plesset equation (Church model, 1995), taking into account the protein/lipid shell layer as a thick, mass-conserving incompressible fluid surrounded by incompressible blood-like fluid. The equation of motion was solved numerically to characterize the fundamental and second harmonic components of the backscattered pressure. Results show a significant advantage in using the second harmonic component for echo PIV, especially for small bubble sizes less than 3 microm in diameter at 2.2 MHz frequency. The effect of the shell thickness ranging from 10 to 500 nm on the vibration amplitude of the bubble was examined and it is shown that the presence of the shell requires mechanical index (MI)0.2 of incident pressure amplitude to improve bubble detectability. Analysis of the effect of pulse length shows a tradeoff between axial resolution (short pulse length) and bubble detectability (longer pulse length) will most likely be required. The effect of varying MI between 0.1 and 0.6 was also studied at a center frequency of 2.2 MHz and the results indicate that the resonance of the second harmonic is maximized for bubbles with diameter of approximately 2.75 microm. Bubble non-linearities at MI0.2 induced a resonant frequency shift away from the integer multiple of the incident frequency in the second harmonic backscatter. For a given bubble size, there is a combination of optimal incident frequency and mechanical index range that maximizes the ratio of the second harmonic compared to the fundamental. This resonant frequency decreases with increasing bubble radius. Further, a narrow bandwidth pulse is shown to increase signal strength. Both of these effects may cause conflict with factors governing spatial resolution. Optimization of the incident frequency, microbubble size and mechanical index to enhance bubble detectability will depend on the particular clinical application. These theoretical predictions provide further understanding of the physics behind our echo PIV technique, and should be useful for guiding the design of echo PIV systems.

We present the results of an experimental study in which a planar laminar jet of air was forced by an array of micro-electromechanical systems (MEMS) micro-actuators. In the absence of forcing, the velocity profile of the experimental jet matched the classic analytic solution. Driving actuators on either side of the jet in-phase or anti-phase, respectively, excited the symmetric or anti-symmetric mode of instability of the jet. Asymmetric forcing, using MEMS actuators on only one side of the jet, was also investigated.

The combination of ultrasound echo images with digital particle image velocimetry (DPIV) methods has resulted in a two-dimensional, two-component velocity field measurement technique appropriate for opaque flow conditions including blood flow in clinical applications. Advanced PIV processing algorithms including an iterative scheme and window offsetting were used to increase the spatial resolution of the velocity measurement to a maximum of 1.8 mm×3.1 mm. Velocity validation tests in fully developed laminar pipe flow showed good agreement with both optical PIV measurements and the expected parabolic profile. A dynamic range of 1 to 60 cm/s has been obtained to date.

Doppler ultrasound color M-mode imaging (CMM) has been proposed as a noninvasive means of quantifying diastolic function by measuring flow propagation into the left ventricle. However, the relationship between CMM-derived parameters and underlying fluid dynamics is still unclear. The purpose of this study was to couple high-resolution velocimetry measurements with ultrasound Doppler and CMM measurements in order to shed light on the relationship between CMM flow propagation and inflow dynamics using a simple yet highly reproducible in vitro model of left ventricular inflow. Two Reynolds number conditions were analyzed: 4000 and 6000. Both conditions produced starting jets that formed vortex rings. Average (N = 5) CMM centerline velocities were in agreement with DPIV centerline velocities, although large uncertainty in CMM data was present (uncertainty +/- 10 cm s(-1)). Two flow propagation parameters were extracted from the CMM data: the first utilized an isovelocity as the marker of flow propagation; the second used local peak velocity as the marker. The isovelocity technique followed the flow proximal to the vortex (wavefront) while the peak velocity method followed peak vorticity, and therefore vortex propagation, closely. We conclude that CMM imaging, while limited in measuring absolute velocities, can be utilized to assess inflow vortex ring properties, and thereby provide useful information on diastolic function.

We describe surface-micromachined thermal actuator-based micro-electro-mechanical systems (MEMS) flaps with a length scale of 1,000 /spl mu/m. These flaps were developed for the enhancement of fluid mixing in an experimental study of a planar air jet. The scales of the flow physics required the actuators to be much larger than the typical MEMS scales, and the experiment required an array of 10 flaps (1 cm in length) to be soldered onto a ceramic substrate with high precision. These unusual requirements made it difficult to achieve good assembly yields that could provide large deflections. To improve the yields and deflections, we modified the initial flap design by reducing the size of the solder pads, removing sharp corners, changing the number and the width of the actuator's hot arms, and strengthening the support beams. In addition, we developed a controllable assembly process to reflow tin/lead solder on gold pads, and we used numerical simulations extensively in the design process to improve the performance. The experimental measurements corroborated these simulations: the deflection was increased from 6.4 to 11.1 /spl mu/m under a 5 V applied voltage. Flow velocity measurements showed that these MEMS flaps amplified the natural instabilities of the planar jet.

Background: The accurate measurement of local pulmonary artery compliance in pediatric pulmonary hypertension is an important step toward further understanding the biomechanical and hemodynamic aspects of the disease. The emergence of intravascular ultrasound (IVUS) imaging techniques promises the ability to make such measurements clinically. However, the use of IVUS for compliance measurements has not been validated. Furthermore, confusion exists regarding the most appropriate method to measure compliance. Methods: This study validated IVUS measurements against a laser micrometer standard for 4 elastic tubes of varying compliance. Two methods of quantifying local compliance were explored: The pressure-strain modulus (E p ),(E p (g/cm 2 ) = ΔP × R d /ΔR (Where ΔP is pulse pressure, R d is diastolic radius, and ΔR is systolic minus diastolic radii) and the dynamic compliance (C dyn ),(C dyn (%/100 mm Hg) = [ΔD/(ΔP × D d )] × 10 4 Where ΔD is systolic minus diastolic diameters and D d is diastolic diameter. Results: IVUS diameter measurements agreed well with laser micrometer data although slight overestimation (mean = 3.67% ± 2.78%) was present. Mean values of E p ranged from 353.3 g/cm 2 to 2676.0 g/cm 2 ; mean C dyn values ranged from 5.7% diametric change/100 mm Hg to 39.5% diametric change/100 mm Hg for all tube models. Although mean values of E p and C dyn could be distinguished among the various tubes, the extremely large measurement uncertainty for E p precluded statistical differentiation. The uncertainty in E p increased inversely with the diametric change, indicating a potential limitation of E p associated with stiffening arteries. Conclusions: We conclude that C dyn is a more robust mean of quantifying pediatric pulmonary artery compliance, especially as arteries stiffen with chronic pulmonary hypertension. (J Am Soc Echocardiogr 2002;15:1507-14.)

Stiffening of large arteries is increasingly used as an independent predictor of risk and therapeutic outcome for small artery dysfunction in many diseases including pulmonary hypertension. The molecular mechanisms mediating downstream vascular cell responses to large artery stiffening remain unclear. We hypothesize that high pulsatility flow, induced by large artery stiffening, causes inflammatory responses in downstream pulmonary artery endothelial cells (PAECs) through toll-like receptor (TLR) pathways. To recapitulate the stiffening effect of large pulmonary arteries that occurs in pulmonary hypertension, ultrathin silicone tubes of variable mechanical stiffness were formulated and were placed in a flow circulatory system. These tubes modulated the simulated cardiac output into pulsatile flows with different pulsatility indices, 0.5 (normal) or 1.5 (high). PAECs placed downstream of the tubes were evaluated for their expression of proinflammatory molecules (ICAM-1, VCAM-1, E-selectin and MCP-1), TLR receptors and intracellular NF-κB following flow exposure. Results showed that compared to flow with normal pulsatility, high pulsatility flow induced proinflammatory responses in PAECs, enhanced TLR2 expression but not TLR4, and caused NF-κB activation. Pharmacologic (OxPAPC) and siRNA inhibition of TLR2 attenuated high pulsatility flow-induced pro-inflammatory responses and NF-κB activation in PAECs. We also observed that PAECs isolated from small pulmonary arteries of hypertensive animals exhibiting proximal vascular stiffening demonstrated a durable ex-vivo proinflammatory phenotype (increased TLR2, TLR4 and MCP-1 expression). Intralobar PAECs isolated from vessels of IPAH patients also showed increased TLR2. In conclusion, this study demonstrates for the first time that TLR2/NF-κB signaling mediates endothelial inflammation under high pulsatility flow caused by upstream stiffening, but the role of TLR4 in flow pulsatility-mediated endothelial mechanotransduction remains unclear.

Background — Accurate determination of pulmonary vascular resistance (PVR) is an important component in the evaluation and treatment of pediatric patients with pulmonary hypertension. We developed a novel technique, based on the concept of flow propagation, to estimate PVR noninvasively. The hypothesis is that changes in PVR cause changes in the velocity propagation (Vel prop ) within the main pulmonary artery and that Vel prop can be quantified using color M-mode imaging. Methods and Results — We tested the hypothesis using mathematical modeling, in vitro experiments, and preliminary clinical studies. The mathematical model showed that pressure and velocity tracings are closely correlated in time and that 6 to 18 ms time resolution was needed to resolve propagation times within typical main pulmonary artery lengths (2 to 5 cm). The in vitro experiments demonstrated that it was feasible to use color M-mode to measure Vel prop and that Vel prop correlated well with downstream resistance [y=(−1.01x)+22.77; R =0.96]. The method was then evaluated on patients undergoing acute pulmonary reactivity testing (n=22 measurements). Good correlation between Vel prop and PVR was found [y=(−1.71x)+26.0; R =0.90; SEE=2.41]. Conclusion — This newly developed method promises to be useful in the noninvasive evaluation of adults and children with pulmonary hypertension.

The near-exit region of confined, unsteady, momentum dominated, non-premixed, chemically reacting jets is investigated computationally using one-step chemistry. Non-premixed flame/wall interaction is manifested in the form of reaction zone broadening. Depending on the value of the stoichiometric mixture fraction, the flame zone moves into or out of the region of strong shear. For flames with large stoichiometric mixture fraction, the flame region is located in closer proximity to the isothermal wall. With increased stoichiometric mixture fraction, reaction thicknesses predicted by asymptotic theory differ from flame zone thicknesses obtained from the simulation databases.

We have recently used Particle Image Velocimetry (PIV) to study the dynamics of vortex propagation in reacting and non-reacting flows. In order to do so, it became necessary to assess the uncertainty in PIV-based vorticity data. A computer simulation was developed to investigate how uncertainty propagates throughout the post-processing, numerical data smoothing, and vorticity calculating algorithms commonly used in the analysis of PIV data. Results indicate that the average uncertainty in vorticity per interrogation cell (normalized to the average vorticity, and then surface averaged), for a simple vortex, can be reduced to approximately ±4% with appropriate measures. This value was obtained using PIV autocorrelation software, a local regression technique combined with a Gaussian-smoothing filter. Our best experimental results (these areas with no lost or spurious vectors) are consistent with Stoke’s theorem.

The objective of this work is to understand the fluid dynamics in the interaction of large scale, three-dimensional vortex structures and transitional diffusion flames in a microgravity environment. The vortex structures are used to provide a known perturbation of the type used in passive and active shear layer control techniques. 'Passive techniques' refers to manipulation of the system geometry to influence the three dimensional dynamics of vortex structures, and 'active' refers to any technique which adds energy (acoustic or kinetic) to the flow to influence the shear layer vortex dynamics. In this work the passive forcing is provided by an elliptic jet cross-section, and the active forcing is incorporated by perturbing the jet velocity using a loudspeaker in the plenum section.

An unusual phenomenon has been observed in a methane jet diffusion flame subjected to axial acoustic forcing. At specific excitation frequencies and amplitudes, the driven flame splits into a central jet and one or two side jets. The splitting is accompanied by a partial detachment of the flame from the nozzle exit, a shortening of the flame by a factor of 2, and a change from the common yellow color of soot radiation to a clear blue flame. Such a phenomenon may be useful for the control of soot production or product species. The splitting is intermittent in time, bifurcating between the split flame and an ordinary single jet diffusion flame. The experiment consists of an unconfined axisymmetric methane jet formed by a short length of 0.4 cm diameter pipe. The pipe is connected to a large plenum surrounding a bass reflex loudspeaker enclosure that provides the excitation. Conditions producing split and bifurcated flames are presented. The drive frequencies required to cause bifurcation correspond to the first two peaks in the system's frequency response curve. Bifurcating behavior was observed at a wide range of flow rates, ranging from very small flames of Reynolds number 240 up to turbulent lift-off, at Re = 1000, based on the inner pipe diameter. It was not sensitive to nozzle length, but the details of the nozzle tip, such as orifice or pipe geometry, can affect the frequency range.

Three‐dimensional interactions between an elliptic vortex ring and two no‐slip parallel walls are visualized in a numerical simulation and an experiment. The vortex ring induces a surface vorticity layer on the wall which reconnects with the vortex ring. During the interaction core‐area‐varying axial waves are generated and carry the surface layer away from the wall. The vortex ring then becomes two tornado‐like structures with strong upward helical flows near the wall surface, which provides entrainment of the surface fluid into the vortex structure. Similarities between the entrainment mechanisms of vorticity in the simulation and dye in the experiment from the surface layer are identified.

A local, small perturbation, linear, inviscid stability analysis is applied to co-flowing reacting shear layers downstream of a bluff body flame holder. Velocity and density profiles are taken from premixed flame experiments. Linear stability theory is employed to determine the regions of transition from absolute to convective instabilities in the wakes of transverse circular cylinder and rectangular bar stabilized flames at two fuel lean conditions, one close to blow off and the other further from blow off. Instabilities in the near wake region of the flame holders are found to be absolute in nature while further downstream in the recirculation zone, the stabilities are of the convective type. Frequencies corresponding to regions of absolute instabilities are determined and compared to previously measured experimental values known to result in vortex shedding.

[Figure][1] [![Graphic][3] ][3][![Graphic][4] ][4][![Graphic][5] ][5] A 33-year-old woman was referred to a cardiologist for an early systolic ejection click and grade III/VI mid peaking systolic murmur at the upper left sternal border. An echocardiogram revealed moderate

For the past six years, a course on flow visualization has been offered to mixed teams of graduate and undergraduate engineering and fine arts photography students at the University of Colorado. The course has significant technical content on flow visualization and photographic techniques, and includes some emphasis on documentation and the interpretation of results, particularly with respect to atmospheric dynamics as revealed by clouds. What makes this course unusual is the emphasis on the production of images for aesthetic purposes: for art. While a number of art/science collaborations are growing worldwide, both in professional and academic communities, typically scientists are expected to contribute technical support while artists produce art. A particularly unusual aspect of this course is that all students are expected to demonstrate both aesthetic sensibility and scientific discipline. Another is that students are not constrained to study specific phenomena or use specific techniques; instead, creativity is required. A major outcome from this course is a series of stunning images. In addition, anecdotal evidence suggests that this course has a lasting impact on students’ perception of fluid physics, which can be contrasted to the effect of traditional introductory fluids courses. This raises the question of whether this impact is significant with respect to students’ understanding and appreciation of fluid mechanics, and if so, what aspect of the flow visualization course is most important? A survey instrument is being designed to quantify whether students’ awareness of fluid mechanics in the world around them changes when they take these courses and if students’ attitudes towards fluids is changed when they take these courses.Copyright © 2009 by ASME

The stabilization of a premixed, turbulent V-shaped flame on a bluff body has been studied using laser Doppler anemometry for two-compenent velocity data, and Rayleigh scattering for point measurements of density. The conditions studied include isothermal flow, a lean ethylene flame at equivalence ratio φ = 0.62, and a very lean (φ = 0.54) flame that is close to blow off for the free stream velocity of 6 m/s. Examination of velocity and density fluctuation spectra and fluctuation intensity contour maps reveals the first quantitative evidence of vortex shedding in the wake of a V-shaped flame under unperturbed free stream conditions. Vortex shedding accompanied by high periodic fluctuation intensities have been clearly identified in the cases of a very lean flame stabilized on a bar (6 × 3mm) and on a rod (6 mm diameter), and in a slightly richer bar stablized case, but not as clearly for a richer rod-stabilized case. It is suggested that vortex shedding may play a role in the blowoff process, and that the assumption of steady recirculation is not always valid.