1. The effect of vitamin D on rat intestinal plasma membrane Ca-pump mRNA
- Author
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Donald E. Sykes, Jay M. Zelinski, and Milton M. Weiser
- Subjects
Male ,medicine.medical_specialty ,Calcitriol ,Enterocyte ,Cellular differentiation ,Biophysics ,Biological Transport, Active ,Biology ,Biochemistry ,vitamin D deficiency ,Random Allocation ,Internal medicine ,Intestine, Small ,medicine ,Vitamin D and neurology ,Animals ,RNA, Messenger ,Northern blot ,Molecular Biology ,Calcium metabolism ,Messenger RNA ,Microvilli ,Cell Membrane ,Nucleic Acid Hybridization ,Cell Biology ,Blotting, Northern ,Vitamin D Deficiency ,medicine.disease ,Rats ,medicine.anatomical_structure ,Endocrinology ,Calcium ,DNA Probes ,medicine.drug - Abstract
The effects of vitamin D on steady-state levels of rat intestinal Ca-pump mRNA were examined in RNA extracted from isolated cell fractions of the crypt-to-villus gradient of differentiation. Northern blots revealed three different size mRNAs. Vitamin D deficient animals showed a decrease in these Ca-pump mRNAs, which increased markedly after 1,25-(OH)2D3 repletion, particularly for the villus cell. The data suggest that one of the effects of 1,25-(OH)2D3 may be to modulate enterocyte Ca-pump mRNA and that this effect is partly dependent on the stage of cell differentiation.
- Published
- 1991
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