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Submarine Groundwater Discharge (SGD) is recognized as a major source of water and solutes to the coastal ocean, and it is particularly relevant in arid or semi-arid zones. SGD is generally defined as the flow of groundwater from continental margins to the coastal ocean, including thus both fresh groundwater from aquifer recharge and seawater recirculation through the coastal aquifer. Due to its high heterogeneity both in space and time, SGD is difficult to detect and quantify. As a consequence, numerous methods to study SGD have been developed over the last decades. These approaches mainly include hydrogeological approaches, geophysical techniques, direct seepage measurements, and the use of geochemical tracers. Each method presents its challenges, limitations, and advantages and each one works on different spatial and temporal scales, thus targeting different components of SGD. Therefore, comparing SGD studies with estimates derived from different methods is often complex and misleading if the characteristics and assumptions of each quantification technique are not taken into account. This highlights the need to conduct studies comparing SGD derived from different methods, not only to obtain more accurate SGD estimates but also to obtain instrumental information on the characteristics of the estimated fluxes. To this aim, a combined use of different approaches to estimate SGD was applied in a Mediterranean coastal lagoon (Mar Menor, Spain), including direct measurements with seepage meters, radium isotopes, and radon mass balance, 224Ra/228Th disequilibrium in coastal sediments, radon vertical profiles in porewater sediments, and hydrologic modeling. Mar Menor is Europe's biggest saline coastal lagoon, and it is connected to a highly anthropized quaternary aquifer. In this coastal system, SGD is likely playing a major role in the eutrophication of the lagoon. However, despite the economic and biological importance of this lagoon, data about this system is still incomplete, and mostly only hydrological modeling has been performed. Keywords: Submarine Groundwater Discharge, radioactive tracers, seepage meters, porewater exchange, hydrological modeling.

The Mar Menor coastal lagoon (Spain) is a critical ecological and socioeconomic ecosystem and the first in Europe to be granted rights of personhood. However, pollution from past and present activities such as mining, agriculture, urbanization, and tourism threatens its health and ecological stability. Previous research has shown the importance of metal contamination in the lagoon and its link to nearby mining activities, but little consideration has been given to historical changes in this industry and in other potential metal sources. In this work, metal concentrations have been analyzed in 12 sediment cores dated with 210Pb, allowing the reconstruction of the recent (last ~150 years) metal contamination in the lagoon. The main metal sources have been identified by using multivariate statistical methods. Metal contamination from mining activities (point-source pollution) peaked in the mid-20th century, whereas nonpoint-source metal contamination reached its highest level in more recent decades. Despite the current decrease in metal deposition trends, concentrations in surface sediments still exceed sediment quality and ecotoxicological thresholds in areas close to former mining sites. Therefore, they need to be considered in future management strategies, which should also include the evaluation of sources and processes that are still supplying them to the lagoon.

The objective of the OPAL (ref: PID2019-110311RB-C22) project is to identify and assess the major pathways delivering nutrients, trace metals and pollutants originated from anthropogenic activities to coastal Mediterranean lagoons connected to intensively used aquifers and their consequences on the lagoon geochemical cycles. One of its goals is “To assess of the role of storms and episodic events in the input of nutrients, metals and pollutants”. Within this framework, the capability of the hydrodynamic model Regional Ocean Model System (ROMS) to simulate fast increase of the Mar Menor coastal lagoon sea level due to extreme flood was tested. In September 2019 the stronger storm and flood registered in the Spanish Levantine area in 87 years occurred. In only 13 hours the sea level of the Mar Menor hypersaline lagoon of 135 Km2 increased 0.6 meters, thus creating a strong halocline due to the fresh water that entered from the floods. The sea level took near 7 days to restore. The morphology of the three outlets communicating the lagoon with the Mediterranean Sea determined their water flux going out the lagoon.This kind of extreme events produces significant enhancements of the inputs of nutrients, metals and pollutants to the lagoon water column coming from an intensive agriculture watershed. To understand the hydrodynamics behind this phenomenon, the ROMS model was used to simulate this episode. Simulations were validated against sea level, salinity and temperature recorded in the water and included surface water inflow from the land, wet/dry areas, bidirectional nesting, water exchange with the Mediterranean Sea and air/water interaction. The result obtained show the robustness of ROMS simulating this kind of events with two layer halocline system formed due to the fresh water inflow from land in a hypersaline water mass.

Common genetic variants ofFOXP2may contribute to schizophrenia vulnerability, but controversial results have been reported for this proposal. Here we evaluated the potential impact of the commonFOXP2rs2396753 polymorphism in schizophrenia. It was previously reported to be part of a risk haplotype for this disease and to have significant effects on gray matter concentration in the patients. We undertook the first examination into whether rs2396753 affects the brain expression ofFOXP2and a replication study of earlier neuroimaging findings of the influence of this genetic variant on brain structure.FOXP2expression levels were measured in postmortem prefrontal cortex samples of 84 male subjects (48 patients and 36 controls) from the CIBERSAM Brain and the Stanley Foundation Array Collections. High-resolution anatomical magnetic resonance imaging was performed on 79 male subjects (61 patients, 18 controls) using optimized voxel-based morphometry. We found differences inFOXP2expression and brain morphometry depending on the rs2396753, relating lowFOXP2mRNA levels with reduction of gray matter density. We detected an interaction between rs2396753 and the clinical groups, showing that heterozygous patients for this polymorphism have gray matter density decrease and lowFOXP2expression comparing with the heterozygous controls. This study shows the importance of independent replication of neuroimaging genetic studies ofFOXP2as a candidate gene in schizophrenia. Furthermore, our results suggest that theFOXP2rs2396753 affects mRNA levels, thus providing new knowledge about its significance as a potential susceptibility polymorphism in schizophrenia. The genomic DNA and RNA samples corresponding to the Array Collection were donated by the Stanley Medical Research Institute Brain Collection courtesy of Drs. Michael B. Knable, E. Fuller Torrey, Maree J. Webster, and Robert H. Yolken. The postmortem human brain tissues were donated by the Brain Collections of the Spanish National Network for Research in Mental Health CIBERSAM. The authors also thank the collaboration of the staff members of the Basque Institute of Legal Medicine, Sant Joan de Deu Foundation and the Psychiatry Unit of Hospital Clinico of Valencia. XHC was supported by a postdoctoral fellowship from CONACYT, Mexico; NSO and JGJ were recipients of research contracts from CIBERSAM, Spain.

This study analyses nautical harbour activities and their spatial distribution using a high-resolution method of wind-wave hindcasting, in order to identify the location of safe and sheltered anchorage areas. We apply this methodological approach to evaluate the ancient harbour of Carthago Nova from the Punic period to the Late Roman period (third century BC–fourth century AD). Literary sources have defined Carthago Nova (Cartagena, Spain) as the only natural harbour of Hispania and one of the best in the Mediterranean, with Escombreras Island as the main natural feature that protects the harbour. Due to the scarcity of archaeological evidence and the transformation the harbour has undergone over time, this study has been considered necessary and carried out in order to supersede the current general and anachronic observations of wind-wave effects. Through an interdisciplinary approach, we analyse one of the main factors that determines the safe anchorage of ships: coastal wind waves. The modelling and simulation of waves have been applied using the SWAN model, paleo-topographic reconstruction, and maritime archaeological data. By means of GIS spatial analysis, an Anchorage Safety Index has been established that computes data from paleo-bathymetry, wind, and simulated wave height. This high-resolution analysis allows us to assess in detail the impact of coastal and island topography on nautical activities inside ancient harbours.

Alteration of centrosome function and dynamics results in major defects during chromosome segregation and is associated with primary autosomal microcephaly (MCPH). Despite the knowledge accumulated in the last few years, why some centrosomal defects specifically affect neural progenitors is not clear. We describe here that the centrosomal kinase PLK1 controls centrosome asymmetry and cell fate in neural progenitors during development. Gain- or loss-of-function mutations in Plk1, as well as deficiencies in the MCPH genes Cdk5rap2 (MCPH3) and Cep135 (MCPH8), lead to abnormal asymmetry in the centrosomes carrying the mother and daughter centriole in neural progenitors. However, whereas loss of MCPH proteins leads to increased centrosome asymmetry and microcephaly, deficient PLK1 activity results in reduced asymmetry and increased expansion of neural progenitors and cortical growth during mid-gestation. The combination of PLK1 and MCPH mutations results in increased microcephaly accompanied by more aggressive centrosomal and mitotic abnormalities. In addition to highlighting the delicate balance in the level and activity of centrosomal regulators, these data suggest that human PLK1, which maps to 16p12.1, may contribute to the neurodevelopmental defects associated with 16p11.2–p12.2 microdeletions and microduplications in children with developmental delay and dysmorphic features., JGM and DMA received predoctoral contracts from the Ministry of Education of Spain (FPI grant BES-2016-077901). This work was supported by Grant PID2019-104763RB-I00 and Ramón y Cajal contract (RYC-2014-15991), both from MINECO/AEI/FEDER (EU) to EP; and grants from the European Commission Seventh Framework Programme (ERA-NET NEURON8-Full-815-094), AEI-MICIU/FEDER (RTI2018-095582-B-I00 and RED2018-102723- T), and the iLUNG programme from the Comunidad de Madrid (B2017/BMD-3884) to MM. CNIO is a Severo Ochoa Center of Excellence (AEI-MICIU CEX2019-000891-S).

The use of dispersive liquid&ndash, liquid microextraction (DLLME) is proposed for the preconcentration of thirteen lipophilic marine toxins in seawater samples. For this purpose, 0.5 mL of methanol and 440 &micro, L of chloroform were injected into 12 mL of sample. The enriched organic phase, once evaporated and reconstituted in methanol, was analyzed by reversed-phase liquid chromatography with triple-quadrupole tandem mass spectrometry. A central composite design multivariate method was used to optimize the interrelated parameters affecting DLLME efficiency. The absence of any matrix effect in the samples allowed them to be quantified against aqueous standards. The optimized procedure was validated by recovery studies, which provided values in the 82&ndash, 123% range. The detection limits varied between 0.2 and 5.7 ng L&minus, 1, depending on the analyte, and the intraday precision values were in the 0.1&ndash, 7.5% range in terms of relative standard deviation. Ten water samples taken from different points of the Mar Menor lagoon were analyzed and were found to be free of the studied toxins.

Study of ocean processes is important to understanding climatic variability especially on the productive upper water-column. Ocean currents regulate the climate, it captures CO\(_2\) from the atmosphere and oxygen is generated by its plankton communities, all of which are part of the global environmental cycle which are being impacted by anthropogenic change. Much of the ocean, however, remains unexplored especially the bio-geochemical processes in the water-column which need to be examined at scale. Satellite remote sensing captures only surface effects while expensive research vessels can only make discrete observations in finite periods of time. Our work with networked marine robotics in the aerial, surface and underwater domains is at the vanguard of a new approach to scientific observation, which brings together technology to enable vessels and robots to work in tandem for capturing synoptic views of open ocean phenomena. We describe a cruise in the Spring of 2018 in the open waters of the Pacific where we employed a fleet of autonomous robots for simultaneous observations of mesoscale and sub-mesoscale features of an unexplored frontal zone. We articulate our approach to multi-vehicle coordination and challenges that lie ahead for research in this harsh domain.

Congenital microcephaly (MCPH) is a neurodevelopmental disease associated to mutations in genes encoding proteins involved in centrosomal and chromosomal dynamics during mitosis. Detailed MCPH pathogenesis at the cellular level is still elusive given the diversity of MCPH genes and lack of comparative in vivo studies. By generating a series of CRISPR/Cas9-mediated genetic knockouts we report here that, whereas defects in spindle pole proteins (ASPM, MCPH5) result in mild microcephaly during development, lack of centrosome (CDK5RAP2, MCPH3) or centriole (CEP135, MCPH8) regulators induces delayed chromosome segregation and chromosomal instability in neural progenitors (NPs). Our novel mouse model of MCPH8 suggests that Cep135 deficiency results in centriole duplication, TP53 activation and cell death of NPs. Trp53 ablation in a Cep135-deficient background prevents cell death, but not microcephaly, and leads to subcortical heterotopias, a malformation seen in MCPH8 patients. These results suggest that microcephaly in some MCPH patients can arise from the lack of adaptation to centriole defects in NPs and may lead to architectural defects if chromosomally unstable cells are not eliminated during brain development.

Autosomal Recessive Primary Microcephaly (MCPH) is a rare disease associated to proteins involved in centrosomal and spindle dynamics including Cep135 (MCPH8). Although Cep135 has been associated to centriolar assembly, the mechanisms associated to the pathogenesis underlying MCPH8 mutations are unclear. By using a series of CRISPR/Cas9-edited murine Cep135 alleles, we report here that lack of Cep135 results in perinatal lethality accompanied by significant microcephaly in a dosis-dependent manner. Cep135 deficiency, but not that of other centrosomal microcephaly proteins such as Aspm or Cdk5rap2, induces centrosome duplication defects, and perturbed centriole structure and dynamics. Whereas other cell types are able to quickly adapt to these defects, neural progenitors display a prolonged response leading to chromosomal instability and cell death in later developmental stages. Genetic ablation of Trp53 in these mutant embryos prevents apoptotic cell death but does not rescue the microcephaly induced by Cep135 loss. These results suggest that microcephaly can arise from the lack of adaptation to centriole defects in neural progenitors of the developing neocortex in a p53-independent manner.

The Coastal Ocean Observation System of Murcia Region (OOCMUR) was established in 2008 as a major scientific and technological infrastructure in Spain with the main objective of studying the impact of global climate change in the Mediterranean. The coastal lagoon of Mar Menor in southeast Spain was chosen as the first region to be monitored because it is one of the most hypersaline coastal lagoons in the Mediterranean, with a limited exchange of water with the open sea, and it is the largest in Europe. Wireless sensor networks (WSNs) offer an efficient and innovative solution for oceanographic monitoring, allowing a higher density sensor deployment, at a lower cost. This paper presents the design of an ad hoc WSN system and a control software for Mar Menor monitoring using a buoy structure with sensors, energy harvesting, and communications platform. The study focuses on the oceanographic interest of the selected marine area, details of network deployment, the custom-designed sensor nodes, and the results of system operation.

The OTX2 homeoprotein transcription factor is expressed in the dopaminergic neurons of the ventral tegmental area, which projects to limbic structures controlling complex behaviors. OTX2 is also produced in choroid plexus epithelium, from which it is secreted into cerebrospinal fluid and transferred to limbic structure parvalbumin interneurons. Previously, adult male mice subjected to early-life stress were found susceptible to anxiety-like behaviors, with accompanying OTX2 expression changes in ventral tegmental area or choroid plexus. Here, we investigated the consequences of reduced OTX2 levels in Otx2 heterozygote mice, as well as in Otx2+/AA and scFvOtx2tg/0 mouse models for decreasing OTX2 transfer from choroid plexus to parvalbumin interneurons. Both male and female adult mice show anxiolysis-like phenotypes in all three models. In Otx2 heterozygote mice, we observed no changes in dopaminergic neuron numbers and morphology in ventral tegmental area, nor in their metabolic output and projections to target structures. However, we found reduced expression of parvalbumin in medial prefrontal cortex, which could be rescued in part by adult overexpression of Otx2 specifically in choroid plexus, resulting in increased anxiety-like behavior. Taken together, OTX2 synthesis by the choroid plexus followed by its secretion into the cerebrospinal fluid is an important regulator of anxiety-related phenotypes in the mouse.

The prefrontal cortex (PFC) continues its development during adolescence and alterations in its structure and function, particularly of inhibitory networks, have been detected in schizophrenic patients. Since cannabis use during adolescence is a risk factor for this disease, our main objective was to investigate whether THC administration during this period might exacerbate alterations in prefrontocortical inhibitory networks in mice subjected to a perinatal injection of MK801 and postweaning social isolation. This double-hit model (DHM) combines a neurodevelopmental manipulation and the exposure to an aversive experience during early life; previous work has shown that DHM mice have important alterations in the structure and connectivity of PFC interneurons. In the present study we found that DHM had reductions in prepulse inhibition of the startle reflex (PPI), GAD67 expression and cingulate 1 cortex volume. Interestingly, THC by itself induced increases in PPI and decreases in the dendritic complexity of somatostatin expressing interneurons. Both THC and DHM reduced the density of parvalbumin expressing cells surrounded by perineuronal nets and, when combined, they disrupted the ratio between the density of puncta expressing excitatory and inhibitory markers. Our results support previous work showing alterations in parameters involving interneurons in similar animal models and schizophrenic patients. THC treatment does not modify further these parameters, but changes some others related also to interneurons and their plasticity, in some cases in the opposite direction to those induced by the DHM, suggesting a protective effect.

Background During last years, there has been an intensive search for blood biomarkers in schizophrenia to assist in diagnosis, prognosis and clinical management of the disease. Methods In this study, we first conducted a weighted gene coexpression network analysis to address differentially expressed genes in peripheral blood from patients with chronic schizophrenia (n?=?30) and healthy controls (n?=?15). The discriminating performance of the candidate genes was further tested in an independent cohort of patients with first-episode schizophrenia (n?=?124) and healthy controls (n?=?54), and in postmortem brain samples (cingulate and prefrontal cortices) from patients with schizophrenia (n?=?34) and healthy controls (n?=?35). Results The expression of the Eukaryotic Translation Initiation Factor 2D (EIF2D) gene, which is involved in protein synthesis regulation, was increased in the chronic patients of schizophrenia. On the contrary, the expression of the Thymocyte Selection-Associated High Mobility Group Box (TOX) gene, involved in immune function, was reduced. EIF2D expression was also altered in first-episode schizophrenia patients, but showing reduced levels. Any of the postmortem brain areas studied did not show differences of expression of both genes. Conclusions EIF2D and TOX are putative blood markers of chronic patients of schizophrenia, which expression change from the onset to the chronic disease, unraveling new biological pathways that can be used for the development of new intervention strategies in the diagnosis and prognosis of schizophrenia disease. Acknowledgments: This work was supported by Fondo de Investigación Sanitaria, Ministerio de Economía y Competitividad, Spain (PI10/01399, PI13/00447; PI17/00402, co-financed by FEDER) to J. Sanjuan and M.D. Moltó; Generalitat Valenciana PROMETEO Excellence Program, Spain (PROMETEO2016/082) to J Sanjuán. J Gilabert-Juan and N. Sebastiá-Ortega were recipients of research contracts from CIBERSAM, Spain. The RNA samples donated bythe Stanley Medical Research Institute Brain Collection were courtesy of Drs. Michael B. Knable, E. Fuller Torrey, Maree J. Webster, and Robert H. Yolken. The authors also thank the collaboration of the staff members of the hospitals.

A distinctive feature of neocortical development is the highly coordinated production of different progenitor cell subtypes, which are critical for ensuring adequate neurogenic outcome and the development of normal neocortical size. To further understand the mechanisms that underlie neocortical growth, we focused our studies on the microcephaly gene Mcph1, and we report here that Mcph1 (1) exerts its functions in rapidly dividing apical radial glial cells (aRGCs) during mouse neocortical development stages that precede indirect neurogenesis; (2) is expressed at mitochondria; and (3) controls the proper proliferation and survival of RGCs, potentially through crosstalk with cellular metabolic pathways involving the stimulation of mitochondrial activity via VDAC1/GRP75 and AKT/HK2/VDAC1 and glutaminolysis via ATF4/PCK2. We currently report the description of a MCPH-gene implication in the interplay between bioenergetic pathways and neocortical growth, thus pointing to alterations of cellular metabolic pathways, in particular glutaminolysis, as a possible cause of microcephalic pathogenesis.

The OTX2 homeoprotein transcription factor is expressed in the dopaminergic neurons of the ventral tegmental area, which projects to limbic structures controlling complex behaviors. OTX2 is also produced in choroid plexus epithelium, from which it is secreted into cerebrospinal fluid and transferred to limbic structure parvalbumin interneurons. Previously, adult male mice subjected to early-life stress were found susceptible to anxiety-like behaviors, with accompanying OTX2 expression changes in ventral tegmental area or choroid plexus. Here, we investigated the consequences of reduced OTX2 levels in Otx2 heterozygote mice, as well as in Otx2

This paper describes the BUSCAMOS-Oil monitoring system, which is a robotic platform consisting of an autonomous surface vessel combined with an underwater vehicle. The system has been designed for the long-term monitoring of oil spills, including the search for the spill, and transmitting information on its location, extent, direction and speed. Both vehicles are controlled by two different types of bio-inspired neural networks: a Self-Organization Direction Mapping Network for trajectory generation and a Neural Network for Avoidance Behaviour for avoiding obstacles. The systems' resilient capabilities are provided by bio-inspired algorithms implemented in a modular software architecture and controlled by redundant devices to give the necessary robustness to operate in the difficult conditions typically found in long-term oil-spill operations. The efficacy of the vehicles' adaptive navigation system and long-term mission capabilities are shown in the experimental results.

This paper presents a report from a cruise onboard the R/V Falkor oceanographic vessel from the Schmidt Ocean Institute. The goal of this cruise was to demonstrate a novel approach to observe the ocean with multiple underwater, surface, and aerial vehicles, as well with the R/V Falkor also used as the base and control center for all assets. We describe the planning phase leading up to the cruise, the technical approach, developments and timeline of results and decisions made throughout the cruise. Our approach combines a set of new technologies that enabled scientists and engineers to obtain a synoptic view of the study area, with adjustable spatial and temporal resolution, and to compare data collected in near real-time to the outputs of computational models. This approach was applied to map the Pacific Ocean’s Subtropical front with unprecedented spatial and temporal resolutions. © 2018 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.

Auditory hallucinations (AH) are clinical hallmarks of schizophrenia, however little is known about molecular genetics of these symptoms. In this study, gene expression profiling of postmortem brain samples from prefrontal cortex of schizophrenic patients without AH (SNA), patients with AH (SA) and control subjects were compared. Genome-wide expression analysis was conducted using samples of three individuals of each group and the Affymetrix GeneChip Human-Gene 1.0 ST-Array. This analysis identified the Axon Guidance pathway as one of the most differentially expressed network among SNA, SA and CNT. To confirm the transcriptome results, mRNA level quantification of seventeen genes involved in this pathway was performed in a larger sample. PLXNB1, SEMA3A, SEMA4D and SEM6C were upregulated in SNA or SA patients compared to controls. PLXNA1 and SEMA3D showed down-regulation in their expression in the patient's samples, but differences remained statistically significant between the SNA patients and controls. Differences between SNA and SA were found in PLXNB1 expression which is decreased in SA patients. This study strengthens the contribution of brain plasticity in pathophysiology of schizophrenia and shows that non-hallucinatory patients present more alterations in frontal regions than patients with hallucinations concerning neural plasticity.

Down syndrome (DS) is the most common chromosomal aneuploidy. Although trisomy on chromosome 21 can display variable phenotypes, there is a common feature among all DS individuals: the presence of intellectual disability. This condition is partially attributed to abnormalities found in the hippocampus of individuals with DS and in the murine model for DS, Ts65Dn. To check if all hippocampal areas were equally affected in 4-5 month adult Ts65Dn mice, we analysed the morphology of dentate gyrus granule cells and cornu ammonis pyramidal neurons using Sholl method on Golgi-Cox impregnated neurons. Structural plasticity has been analysed using immunohistochemistry for plasticity molecules followed by densitometric analysis (Brain Derived Neurotrophic Factor (BDNF), Polysialylated form of the Neural Cell Adhesion Molecule (PSA-NCAM) and the Growth Associated Protein 43 (GAP43)). We observed an impairment in the dendritic arborisation of granule cells, but not in the pyramidal neurons in the Ts65Dn mice. When we analysed the expression of molecules related to structural plasticity in trisomic mouse hippocampus, we observed a reduction in the expression of BDNF and PSA-NCAM, and an increment in the expression of GAP43. These alterations were restricted to the regions related to dentate granule cells suggesting an interrelation. Therefore the impairment in dendritic arborisation and molecular plasticity is not a general feature of all Down syndrome principal neurons. Pharmacological manipulations of the levels of plasticity molecules could provide a way to restore granule cell morphology and function.

Down Syndrome, with an incidence of one in 800 live births, is the most common genetic alteration producing intellectual disability. We have used the Ts65Dn model, that mimics some of the alterations observed in Down Syndrome. This genetic alteration induces an imbalance between excitation and inhibition that has been suggested as responsible for the cognitive impairment present in this syndrome. The hippocampus has a crucial role in memory processing and is an important area to analyze this imbalance. In this report we have analysed, in the hippocampus of Ts65Dn mice, the expression of synaptic markers: synaptophysin, vesicular glutamate transporter-1 and isoform 67 of the glutamic acid decarboxylase; and of different subtypes of inhibitory neurons (Calbindin D-28k, parvalbumin, calretinin, NPY, CCK, VIP and somatostatin). We have observed alterations in the inhibitory neuropil in the hippocampus of Ts65Dn mice. There was an excess of inhibitory puncta and a reduction of the excitatory ones. In agreement with this observation, we have observed an increase in the number of inhibitory neurons in CA1 and CA3, mainly interneurons expressing calbindin, calretinin, NPY and VIP, whereas parvalbumin cell numbers were not affected. These alterations in the number of interneurons, but especially the alterations in the proportion of the different types, may influence the normal function of inhibitory circuits and underlie the cognitive deficits observed in DS.

Introduction Chronic stress induces dendritic atrophy and decreases spine density in excitatory hippocampal neurons, although there is also ample evidence indicating that the GABAergic system is altered in the hippocampus after this aversive experience. Chronic stress causes dendritic remodeling both in excitatory neurons and interneurons in the medial prefrontal cortex and the amygdala. Methods In order to know whether it also has an impact on the structure and neurotransmission of hippocampal interneurons, we have analyzed the dendritic arborization, spine density, and the expression of markers of inhibitory synapses and plasticity in the hippocampus of mice submitted to 21 days of mild restrain stress. The analyses were performed in GIN mice, a strain that displays EGFP‐labeled interneurons. Results We observed a significant decrease in the dendritic arborization of interneurons in the CA1 region, which did not occur in those in CA3. We found neither changes in dendritic spine density in these regions nor alterations in the number of EGFP‐positive interneurons. Nevertheless, the expression of glutamic acid decarboxylase 67 was reduced in different layers of CA1 and CA3 regions of the hippocampus. No significant changes were found in the expression of the polysialylated form of the neural cell adhesion molecule (PSA‐NCAM) or synaptophysin. Conclusions Chronic stress reduces the interneuronal dendritic arborization in CA1 region of the hippocampus but not in CA3.

Over the last decade, ocean sunfish movements have been monitored worldwide using various satellite tracking methods. This study reports the near-real time monitoring of fine-scale (< 10 m) behaviour of sunfish. The study was conducted in southern Portugal in May 2014 and involved satellite tags and underwater and surface robotic vehicles to measure both the movements and the contextual environment of the fish. A total of four individuals were tracked using custom-made GPS satellite tags providing geolocation estimates of fine-scale resolution. These accurate positions further informed sunfish areas of restricted search (ARS), which were directly correlated to steep thermal frontal zones. Simultaneously, and for two different occasions, an Autonomous Underwater Vehicle (AUV) video-recorded the path of the tracked fish and detected buoyant particles in the water column. Importantly, the densities of these particles were also directly correlated to steep thermal gradients. Thus, both sunfish foraging behaviour (ARS) and possibly prey densities, were found to be influenced by analogous environmental conditions. In addition, the dynamic structure of the water transited by the tracked individuals was described by a Lagrangian modelling approach. The model informed the distribution of zooplankton in the region, both horizontally and in the water column, and the resultant simulated densities positively correlated with sunfish ARS behaviour estimator (rs = 0.184, p

Chronic stress in experimental animals induces dendritic atrophy and decreases spine density in principal neurons of the medial prefrontal cortex (mPFC). This structural plasticity may play a neuroprotective role and underlie stress-induced behavioral changes. Different evidences indicate that the prefrontocortical GABA system is also altered by stress and in major depression patients. In the amygdala, chronic stress induces dendritic remodeling both in principal neurons and in interneurons. However, it is not known whether similar structural changes occur in mPFC interneurons. The polysialylated form of the neural cell adhesion molecule (PSA-NCAM) may mediate these changes, because it is known to influence the dendritic organization of adult cortical interneurons. We have analyzed the dendritic arborization and spine density of mPFC interneurons in adult mice after 21 days of restraint stress and have found dendritic hypertrophy in a subpopulation of interneurons identified mainly as Martinotti cells. This aversive experience also decreases the number of glutamate decarboxylase enzyme, 67 kDa isoform (GAD67) expressing somata, without affecting different parameters related to apoptosis, but does not alter the number of interneurons expressing PSA-NCAM. Quantitative retrotranscription-polymerase chain reaction (qRT-PCR) analysis of genes related to general and inhibitory neurotransmission and of PSA synthesizing enzymes reveals increases in the expression of NCAM, synaptophysin and GABA(A)α1. Together these results show that mPFC inhibitory networks are affected by chronic stress and suggest that structural plasticity may be an important feature of stress-related psychiatric disorders where this cortical region, specially their GABAergic system, is altered.

Chronic stress in experimental animals, one of the most accepted models of chronic anxiety and depression, induces structural remodeling of principal neurons in the amygdala and increases its excitation by reducing inhibitory tone. These changes may be mediated by the polysialylated form of the neural cell adhesion molecule (PSA-NCAM), a molecule related to neuronal structural plasticity and expressed by interneurons in the adult CNS, which is downregulated in the amygdala after chronic stress. We have analyzed the amygdala of adult mice after 21 days of restraint stress, studying with qRT-PCR the expression of genes related to general and inhibitory neurotransmission, and of PSA synthesizing enzymes. The expression of GAD67, synaptophysin and PSA-NCAM was also studied in specific amygdaloid nuclei using immunohistochemistry. We also analyzed dendritic arborization and spine density, and cell activity, monitoring c-Fos expression, in amygdaloid interneurons. At the mRNA level, the expression of GAD67 and of St8SiaII was significantly reduced. At the protein level there was an overall reduction in the expression of GAD67, synaptophysin and PSA-NCAM, but significant changes were only detected in specific amygdaloid regions. Chronic stress did not affect dendritic spine density, but reduced dendritic arborization in interneurons of the lateral and basolateral amygdala. These results indicate that chronic stress modulates inhibitory neurotransmission in the amygdala by regulating the expression of molecules involved in this process and by promoting the structural remodeling of interneurons. The addition of PSA to NCAM by St8SiaII may be involved in these changes.

Principal neurons in the adult cerebral cortex undergo synaptic, dendritic, and spine remodeling in response to different stimuli, and several reports have demonstrated that the polysialylated form of the neural cell adhesion molecule (PSA-NCAM) participates in these plastic processes. However, there is only limited information on the expression of this molecule on interneurons and on its role in the structural plasticity of these cells. We have found that PSA-NCAM is expressed in mature interneurons widely distributed in all the extension of the cerebral cortex and have excluded the expression of this molecule in most principal cells. Although PSA-NCAM expression is generally considered a marker of immature neurons, birth-dating analyses reveal that these interneurons do not have an adult or perinatal origin and that they are generated during embryonic development. PSA-NCAM expressing interneurons show reduced density of perisomatic and peridendritic puncta expressing different synaptic markers and receive less perisomatic synapses, when compared with interneurons lacking this molecule. Moreover, they have reduced dendritic arborization and spine density. These data indicate that PSA-NCAM expression is important for the connectivity of interneurons in the adult cerebral cortex and that its regulation may play an important role in the structural plasticity of inhibitory networks.

[EN] Unmanned Underwater Vehicles (UUVs) explore different habitats with a view to protecting and managing them. They are developed to overcome scientific challenges and the engineering problems caused by the unstructured and hazardous underwater environment in which they operate. Their development bears the same difficulties as the rest of service robots (hardware heterogeneity, sensor uncertainty, software complexity, etc.) as well as other particular from the domain, like the underwater environment, energy constraints, and autonomy. This article describes the AEGIR UUV, used as a test bed for implementation of control strategies and oceanographic mission in the Mar Menor area in Spain, which is one of the largest coastal lagoons in Europe. It also describes the development of a tool chain that follows a model-driven approach, which has been used in the design of the vehicle control software as well as a component-based framework that provides the runtime support of the application and enables its flexible deployment in nodes, processes and threads and pre-verification of concurrent behavior., [ES] Los vehículos submarinos no tripulados (Unmanned Underwater Vehicles, UUVs) se diseñan para misiones de monitorización, inspección e intervención. En estudios oceanográficos y de monitorización ambiental son cada vez más demandados por las innumerables ventajas que presentan con respecto a las tecnologías tradicionales. Estos vehículos son desarrollados para superar los retos científicos y los problemas de ingeniería que aparecen en el entorno no estructurado y hostil del fondo marino en el que operan. Su desarrollo no solo conlleva las mismas dificultades que el resto de los robots de servicio (heterogeneidad en el hardware, incertidumbre de los sistemas de medida, complejidad del software, etc.), sino que además se les unen las propias del dominio de aplicación, la robótica submarina: condiciones de iluminación, incertidumbre en cuanto a posición y velocidad, restricciones energéticas, etc. Este artículo describe el UUV AEGIR, un vehículo utilizado como banco de pruebas para la implementación de estrategias de control y misiones oceanográficas. También describe el desarrollo de una cadena de herramientas que sigue un enfoque dirigido por modelos, utilizada en el diseño del software de control del vehículo, así como un framework basado en componentes que proporciona el soporte de ejecución de la aplicación y permite su despliegue flexible en nodos, procesos e hilos y pre-verificación del comportamiento concurrente. Su diseño ha permitido desarrollar, comprobar y añadir los componentes que proporcionan el comportamiento necesario para que el UUV AEGIR pudiera completar con éxito distintos tipos de misiones oceanográficas., Este trabajo ha sido parcialmente financiado por el proyecto financiado por la CICYT del Gobierno Español DIVISAMOS (ref. DPI2009-14744-C03-02) y ViSelTR (ref. TIN2012-39279), así como por el proyecto financiado por la Fundación Séneca de la Región de Murcia MISSION-SICUVA (ref. 15374/PI/10) y el proyecto “Coastal Monitoring System for the Mar Menor Coastal Lagoon (PEPLAN 463.02-08 CLUSTER de la Región de Murcia. Francisco Sánchez Ledesma agradece la financiación recibida por parte del programa de becas FPU del MEC (beca AP2009-5083). Por último, los autores quieren agradecer también a la Armada Española la cesión del vehículo UUV y su posterior ayuda en su reconstrucción.

Summary In highly anthropized watersheds, surface water tributaries may carry unexpected high quantities of radon and radium to coastal lagoons. Investigating submarine groundwater discharge (SGD) with radionuclide tracers is therefore a complex task. In order to quantify SGD and decipher the influence of the different water sources, we combined a radon ( 222 Rn) and short-lived radium ( 223 Ra, 224 Ra) survey with the hydrodynamic modeling of a lagoon. We applied it to the Mar Menor lagoon (SE Spain) where surface water tributaries and undocumented emissaries carry water from groundwater drainage and brines from groundwater desalinization. We identified the areas of influence of the plume of radionuclides from the river, located major areas of SGD and proposed a location for two submarine emissaries. Porewater, i.e. interstitial water from underlying sediments, was found to be the most representative SGD end member, compared to continental groundwater collected from piezometers. Mass balances in winter and summer seasons provided yearly SGD fluxes of water of 0.4–2.2 ⋅ 10 8 m 3 /y ( 222 Rn), 4.4–19.0 ⋅ 10 8 m 3 /y ( 224 Ra) and 1.3 ⋅ 10 8 m 3 /y ( 223 Ra, measured in winter only). Tidal pumping was identified as a main driver for recirculated saline groundwater, while fresh submarine groundwater discharge from the aquifer ranged between 2% and 23% of total SGD.

The tragic Deepwater Horizon accident in the Gulf of Mexico in 2010 as well as the increase in deepwater offshore activity have increased public interest in counter-measures available for sub-surface releases of hydrocarbons. To arrive at proper contingency planning, response managers urge for a system for instant detection and characterization of accidental releases. Along these lines, this paper describes the application of a heterogeneous robotic system of unmanned vehicles: autonomous underwater vehicle (AUV), unmanned surface vehicle (USV) and unmanned aerial vehicle (UAV) extended with the oil spill numerical modeling, visualisation and decision support capabilities. A first set of field experiments simulating oil spill scenarios with Rhodamine WT was held in Croatia during the early autumn 2014. The objectives of this experiment were to test: effectiveness of the system for underwater detection of hydrocarbons, including multi-vehicle collaborative navigation and communication as well as visualisation of the system components.

The Mar Menor is a sheltered and hypersaline lagoon, with salinity ranges between 38 and 51 psu. The lagoon is threatened by several pressures and in the last decades detrimental impact on the natural community structure and dynamics have increased. In the watershed, agricultural practices are rapidly evolving from extensive dry crop farming to intensively irrigated crops, with increasing loads of nutrient and pollutants to the lagoon. Hydrological conditions, nutrients and chlorophyll a concentrations were analysed in 1997 and 2002–2003 in a grid of 20 stations in the lagoon. Different time scales, from daily to inter-annual, were considered. In the considered periods, the dissolved inorganic nitrogen (DIN) increased whilst phosphate decreased significantly. These contrasting patterns depended upon the increased agricultural loading for DIN and were due to the implementation of the wastewater works for phosphates. In 1997 and 2002, the highest nitrate concentrations were usually found on the west coast of the lagoon, close to the mouths of the main watercourses. In parallel, the lowest concentrations were detected at the inner coastline along “La Manga” sandy bar and “El Estacio” channel. Based on weekly data, correlations between chlorophyll a concentrations and environmental variables disagreed with traditional eutrophication models. Relationships between chlorophyll a and nutrients were negative, suggesting that in the short term phytoplankton controlled nutrient concentrations. Moreover, nitrate and phosphorous seemed to alternate as limiting factors. The relationships between chlorophyll a became positive when considering time lags and analysed at longer time scales (monthly or seasonal means), thus suggesting a very rapid response of primary producers to nutrient enrichment. A significant correlation between chlorophyll a concentration and fish larvae density was also found at all time scales analysed, suggesting a top-down control of the trophic web.

We investigated the association between ST8SIAII and schizophrenia in a sample of Spanish origin. We found that the G allele (P=0.044) and the AG genotype (P=0.040) of rs3759916 were associated in females. The ACAG haplotype (rs3759914, rs3759915, rs3759916 and rs2305561) was associated in males (P=0.028).

The exposure to aversive experiences during early life influences brain development and leads to altered behavior. Moreover, the combination of these experiences with subtle alterations in neurodevelopment may contribute to the emergence of psychiatric disorders, such as schizophrenia. Recent hypotheses suggest that imbalances between excitatory and inhibitory (E/I) neurotransmission, especially in the prefrontal cortex and the amygdala, may underlie their etiopathology. In order to understand better the neurobiological bases of these alterations, we studied the impact of altered neurodevelopment and chronic early-life stress on these two brain regions. Transgenic mice displaying fluorescent excitatory and inhibitory neurons, received a single injection of MK801 (NMDAR antagonist) or vehicle solution at postnatal day 7 and/or were socially isolated from the age of weaning until adulthood (3 months old). We found that anxiety-related behavior, brain volume, neuronal structure, and the expression of molecules related to plasticity and E/I neurotransmission in adult mice were importantly affected by early-life stress. Interestingly, many of these effects were potentiated when the stress paradigm was applied to mice perinatally injected with MK801 ("double-hit" model). These results clearly show the impact of early-life stress on the adult brain, especially on the structure and plasticity of inhibitory networks, and highlight the double-hit model as a valuable tool to study the contribution of early-life stress in the emergence of neurodevelopmental psychiatric disorders, such as schizophrenia.

Communications between two autonomous vehicles has been always an important challenge, especially when one of the vehicles is intended for conducting submerged navigation most of its operational time. Underwater communications become in that case the one most common and feasible way for allowing data trasfering underneath the sea surface. Considering low cost of the final vehicle as one of the main features in the concept of this project, a low power low-cost acoustic modem initially intended for environmental sensor applications has been adopted. With the purpose of increasing at maximum the energy reservoir, this modem includes among others, self-wake capabilities and directional transmission. The present work continues in the validation of this acoustic device for application in Underwater Unmanned Vehicles (UUV) in multivehicle configurations. Different solutions for solving the problems associated to the difficulty of conducting robust and reliable data transfering by using this device are presented.

Zinc is an essential trace element that is critical for a large number of structural proteins, enzymatic processes and transcription factors. In the brain, zinc ions are involved in synaptic transmission. The homeostasis of zinc is crucial for cell survival and function, and cells have developed a wide variety of systems to control zinc concentration. Alterations in free zinc concentration have been related with brain dysfunction. Down Syndrome individuals present alterations in free zinc concentration and in some of the proteins related with zinc homeostasis. We have analyzed the amount of free zinc and the zinc chelating protein metallothionein 3 in the astrocytes using primary cultures of the murine model Ts65Dn. We have observed a higher number of zinc positive spots in the cytoplasm of trisomic astrocytes but a decrease in the total concentration of total intracellular free zinc concentration (including the spots) respect to control astrocytes. Using FM1-43 staining, we found that the endocytic function remains unaltered. Therefore, a possible explanation for this lower concentration of free zinc could be the higher concentration of metallothionein 3 present in the cytoplasm of trisomic astrocytes. The blockade of metallothionein 3 expression using an specific siRNA induced an increase in the concentration of free zinc in basal conditions but failed to increase the uptake of zinc after incubation with zinc ions.

The seasonal distribution of plankton in a Mediterranean hypersaline coastal lagoon has been studied through a dataset, comprising the taxonomic composition and the size–abundance distribution of both phyto- and zooplankton, measured by image analysis techniques during a one-year time series of weekly samplings. The studied organisms ranged from small nanoplanktonic heterotrophic flagellates (2 μm diameter) to fish larvae (>2 μm). The phytoplankton annual succession was characterized by a winter period dominated by Rhodomonas spp. and Cryptomonas spp. with Cyclotella spp. as the main diatom represented, a spring phase where diatoms (mainly Cyclotella) were the dominant group with some monospecific blooms of other diatoms (mainly of Chaetoceros sp.), a summer phase characterized by diatoms with blooms of Niztschia closterium, and a post-summer phase where dinoflagellates increased with peaks of Ceratium furca. High densities of the microbial food web elements, flagellates and ciliates, indicate the importance of the microbial loop in the ecosystem. Meroplankton contributed widely to the seasonal character of the zooplankton distribution. Copepods, represented by Oithona nana, Centropages ponticus and Acartia spp. (mainly latisetosa), remained relatively constant throughout the year, exhibiting a lower density in the warmer water period (July–September). At the end of the sampling period, a massive proliferation of copepods (>1000 ind l –1), mainly due to O. nana, took place. The autotrophs to heterotrophs biovolume ratio (A:H) remained lower than 1 throughout the year except when, occasionally, large phytoplankton cells bloomed. Persistent very low values of A:H suggest that additional sources of energy, such as the microbial loop or detrital pathways, would be needed to sustain the high heterotrophic biovolume found in the lagoon.